0 3 The the DT 4 8 zinc zinc NN 9 15 finger finger NN 16 29 transcription transcription NN 30 36 factor factor NN 37 42 Egr-1 egr-1 NN 43 45 is be VBZ 46 55 essential essential JJ 56 59 for for IN 60 63 and and CC 64 73 restricts restrict VBZ 74 89 differentiation differentiation NN 90 95 along along IN 96 99 the the DT 100 110 macrophage macrophage NN 111 118 lineage lineage NN 118 119 . . . 121 123 We we PRP 124 128 have have VBP 129 137 isolated isolate VBN 138 142 cDNA cdna NN 143 149 clones clone NNS 150 152 of of IN 153 160 myeloid myeloid JJ 161 176 differentiation differentiation NN 177 184 primary primary JJ 185 193 response response NN 194 195 ( ( ( 195 198 MyD MyD NNP 198 199 ) ) ) 200 205 genes gene NNS 205 206 , , , 207 216 activated activate VBN 217 219 in in IN 220 223 the the DT 224 231 absence absence NN 232 234 of of IN 235 237 de de FW 238 242 novo novo FW 243 250 protein protein NN 251 260 synthesis synthesis NN 261 270 following follow VBG 271 280 induction induction NN 281 284 for for IN 285 300 differentiation differentiation NN 301 306 along along IN 307 313 either either CC 314 317 the the DT 318 328 macrophage macrophage NN 329 331 or or CC 332 343 granulocyte granulocyte NN 344 351 lineage lineage NN 352 354 in in IN 355 360 human human JJ 361 373 myeloblastic myeloblastic JJ 374 382 leukemia leukemia NN 383 388 HL-60 hl-60 NN 389 394 cells cell NNS 394 395 . . . 396 399 One one CD 400 404 cDNA cdna NN 405 410 clone clone NN 411 413 of of IN 414 415 a a DT 416 423 primary primary JJ 424 432 response response NN 433 437 gene gene NN 437 438 , , , 439 448 expressed express VBN 449 453 upon upon IN 454 464 macrophage macrophage NN 465 480 differentiation differentiation NN 480 481 , , , 482 489 encoded encode VBN 490 493 for for IN 494 499 Egr-1 Egr-1 NNP 499 500 , , , 501 502 a a DT 503 507 zinc zinc NN 508 514 finger finger NN 515 528 transcription transcription NN 529 535 factor factor NN 535 536 . . . 537 540 The the DT 541 546 Egr-1 egr-1 NN 547 551 gene gene NN 552 555 was be VBD 556 564 observed observe VBN 565 567 to to TO 568 570 be be VB 571 588 transcriptionally transcriptionally RB 589 595 silent silent JJ 596 598 in in IN 599 604 HL-60 hl-60 NN 605 610 cells cell NNS 610 611 , , , 612 615 but but CC 616 622 active active JJ 623 625 in in IN 626 631 U-937 u-937 NN 632 635 and and CC 636 638 M1 m1 NN 639 644 cells cell NNS 644 645 , , , 646 649 the the DT 650 656 latter latter JJ 657 660 two two CD 661 666 being be VBG 667 680 predetermined predetermine VBN 681 684 for for IN 685 695 macrophage macrophage NN 696 711 differentiation differentiation NN 711 712 . . . 713 718 Egr-1 egr-1 NN 719 728 antisense antisense JJ 729 738 oligomers oligomer NNS 739 741 in in IN 742 745 the the DT 746 753 culture culture NN 754 759 media medium NNS 760 767 blocked block VBD 768 778 macrophage macrophage NN 779 794 differentiation differentiation NN 795 797 in in IN 798 802 both both CC 803 810 myeloid myeloid JJ 811 819 leukemia leukemia NN 820 824 cell cell NN 825 830 lines line NNS 831 834 and and CC 835 841 normal normal JJ 842 853 myeloblasts myeloblast NNS 853 854 . . . 855 860 HL-60 HL-60 NNP 861 866 cells cell NNS 867 881 constitutively constitutively RB 882 892 expressing express VBG 893 895 an an DT 896 901 Egr-1 egr-1 NN 902 911 transgene transgene NN 912 913 ( ( ( 913 923 HL-60Egr-1 hl-60egr-1 NN 923 924 ) ) ) 925 930 could could MD 931 933 be be VB 934 941 induced induce VBN 942 945 for for IN 946 956 macrophage macrophage NN 956 957 , , , 958 961 but but CC 962 965 not not RB 966 977 granulocyte granulocyte NN 977 978 , , , 979 994 differentiation differentiation NN 994 995 . . . 996 1001 These these DT 1002 1014 observations observation NNS 1015 1023 indicate indicate VBP 1024 1028 that that IN 1029 1039 expression expression NN 1040 1042 of of IN 1043 1048 Egr-1 egr-1 NN 1049 1051 is be VBZ 1052 1061 essential essential JJ 1062 1065 for for IN 1066 1069 and and CC 1070 1079 restricts restrict VBZ 1080 1095 differentiation differentiation NN 1096 1098 of of IN 1099 1110 myeloblasts myeloblast NNS 1111 1116 along along IN 1117 1120 the the DT 1121 1131 macrophage macrophage NN 1132 1139 lineage lineage NN 1139 1140 . . .