0 11 USF-related usf-related JJ 12 25 transcription transcription NN 26 32 factor factor NN 32 33 , , , 34 41 HIV-TF1 HIV-TF1 NNP 41 42 , , , 43 53 stimulates stimulate VBZ 54 67 transcription transcription NN 68 70 of of IN 71 76 human human JJ 77 93 immunodeficiency immunodeficiency NN 94 101 virus-1 virus-1 NN 101 102 . . . 104 107 The the DT 108 121 transcription transcription NN 122 128 factor factor NN 129 136 HIV-TF1 hiv-tf1 NN 136 137 , , , 138 143 which which WDT 144 149 binds bind VBZ 150 152 to to TO 153 154 a a DT 155 161 region region NN 162 167 about about IN 168 170 60 60 CD 171 173 bp bp NN 174 182 upstream upstream RB 183 187 from from IN 188 191 the the DT 192 200 enhancer enhancer NN 201 203 of of IN 204 207 the the DT 208 213 human human JJ 214 230 immunodeficiency immunodeficiency NN 231 238 virus-1 virus-1 NN 239 240 ( ( ( 240 245 HIV-1 HIV-1 NNP 245 246 ) ) ) 246 247 , , , 248 251 was be VBD 252 260 purified purify VBN 261 265 from from IN 266 271 human human JJ 272 273 B B NNP 274 279 cells cell NNS 279 280 . . . 281 288 HIV-TF1 HIV-TF1 NNP 289 292 had have VBD 293 294 a a DT 295 304 molecular molecular JJ 305 311 weight weight NN 312 314 of of IN 315 321 39,000 39,000 CD 321 322 . . . 323 330 Binding binding NN 331 333 of of IN 334 341 HIV-TF1 hiv-tf1 NN 342 344 to to TO 345 348 the the DT 349 352 HIV HIV NNP 353 357 long long JJ 358 366 terminal terminal JJ 367 373 repeat repeat NN 374 375 ( ( ( 375 378 LTR LTR NNP 378 379 ) ) ) 380 389 activated activate VBD 390 403 transcription transcription NN 404 408 from from IN 409 412 the the DT 413 416 HIV HIV NNP 417 425 promoter promoter NN 426 428 in in FW 429 434 vitro vitro FW 434 435 . . . 436 439 The the DT 440 455 HIV-TF1-binding hiv-tf1-binding JJ 456 460 site site NN 461 463 in in IN 464 467 HIV HIV NNP 468 471 LTR LTR NNP 472 475 was be VBD 476 483 similar similar JJ 484 486 to to TO 487 490 the the DT 491 495 site site NN 496 506 recognized recognize VBN 507 509 by by IN 510 518 upstream upstream JJ 519 530 stimulatory stimulatory JJ 531 537 factor factor NN 538 539 ( ( ( 539 542 USF USF NNP 542 543 ) ) ) 544 546 in in IN 547 550 the the DT 551 561 adenovirus adenovirus NN 562 567 major major JJ 568 572 late late JJ 573 581 promoter promoter NN 581 582 . . . 583 594 DNA-binding dna-binding JJ 595 605 properties property NNS 606 608 of of IN 609 616 HIV-TF1 HIV-TF1 NNP 617 626 suggested suggest VBD 627 631 that that IN 632 639 HIV-TF1 hiv-tf1 NN 640 645 might might MD 646 648 be be VB 649 658 identical identical JJ 659 661 or or CC 662 669 related related JJ 670 672 to to TO 673 676 USF USF NNP 676 677 . . . 678 691 Interestingly interestingly RB 691 692 , , , 693 702 treatment treatment NN 703 705 of of IN 706 714 purified purify VBN 715 722 HIV-TF1 hiv-tf1 NN 723 725 by by IN 726 737 phosphatase phosphatase NN 738 745 greatly greatly RB 746 753 reduced reduce VBD 754 757 its its PRP$ 758 769 DNA-binding dna-binding JJ 770 778 activity activity NN 778 779 , , , 780 790 suggesting suggest VBG 791 795 that that IN 796 811 phosphorylation phosphorylation NN 812 814 of of IN 815 822 HIV-TF1 HIV-TF1 NNP 823 826 was be VBD 827 836 essential essential JJ 837 840 for for IN 841 844 DNA DNA NNP 845 852 binding binding NN 852 853 . . . 854 857 The the DT 858 868 disruption disruption NN 869 871 of of IN 872 887 HIV-TF1-binding hiv-tf1-binding JJ 888 892 site site NN 893 900 induced induce VBD 901 902 a a DT 903 905 60 60 CD 905 906 % % NN 907 915 decrease decrease NN 916 918 in in IN 919 922 the the DT 923 928 level level NN 929 931 of of IN 932 945 transcription transcription NN 946 950 from from IN 951 954 the the DT 955 958 HIV HIV NNP 959 967 promoter promoter NN 968 970 in in FW 971 975 vivo vivo FW 975 976 . . . 977 982 These these DT 983 990 results result NNS 991 998 suggest suggest VBP 999 1003 that that IN 1004 1011 HIV-TF1 hiv-tf1 NN 1012 1014 is be VBZ 1015 1023 involved involve VBN 1024 1026 in in IN 1027 1042 transcriptional transcriptional JJ 1043 1053 regulation regulation NN 1054 1056 of of IN 1057 1062 HIV-1 HIV-1 NNP 1062 1063 . . .