765048601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NFATp</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NFATc</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">natural</tok> <tok cat="NN">killer</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD16</tok></cons> <tok cat="NN">ligand</tok> <tok cat="NN">binding</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@28@@oe@16-12-2010
765048602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">putative</tok> <tok cat="NNS">factors</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">couple</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">surface</tok> <tok cat="NNS">receptors</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok></cons> <tok cat="NN">synthesis</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">natural</tok> <tok cat="NN">killer</tok> <tok cat="LRB">(</tok><tok cat="NN">NK</tok><tok cat="RRB">)</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">elucidated</tok><cons cat="NP" ref="i1" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@29@@oe@16-12-2010
765048603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NFATp</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">cyclosporin</tok> <tok cat="NN">A</tok> <tok cat="LRB">(</tok><tok cat="NN">CsA</tok><tok cat="RRB">)</tok><tok cat="JJ">-sensitive</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">regulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">several</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">mediates</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">CD16-induced</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
765048604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">CD16</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Fc</tok> <tok cat="NN">gamma</tok> <tok cat="NN">RIIIA</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="JJ">-induced</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">mRNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">CsA-sensitive</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">Ca(2+)-dependent</tok></cons></cons> <tok cat="NN">mechanism</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
765048605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD16</tok></cons> <tok cat="NNS">ligands</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><tok cat="JJ">NFAT-like</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
765048606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">fast</tok> <tok cat="NNS">kinetics</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">stimulation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">CsA-sensitive</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">Ca(2+)-dependent</tok></cons></cons> <tok cat="NN">mechanism</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">require</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="FW">de</tok> <tok cat="FW">novo</tok></cons> <tok cat="NN">protein</tok> <tok cat="NN">synthesis</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
765048607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NK</tok> <tok cat="NN">cell</tok> <tok cat="NN">NFAT</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytosol</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nonstimulated</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">migrates</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons> <cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><tok cat="NN">stimulation</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">associate</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
765048608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="CD">Two</tok> <tok cat="JJ">distinct</tok> <tok cat="NNS">molecules</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NFATp</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NFATc</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">reported</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">mediate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NFAT</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
765048609@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">supershift</tok> <tok cat="NNS">assays</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NFATp-</tok> <cons cat="ADJP" null="QSTN"></cons><cons cat="NP" ref="i6" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">NFATc- specific</tok> <cons cat="NP" ref="i6" null="RNR"></cons></cons><cons cat="NP" id="i6"><tok cat="NNS">antibodies</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">NK</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons> <cons cat="ADVP"><tok cat="RB">early</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD16</tok></cons> <tok cat="NN">ligand</tok> <tok cat="NN">binding</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="ADVP" syn="COOD"><cons cat="ADVP"><tok cat="RB">primarily</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="IN">if</tok> <tok cat="RB">not</tok></cons> <cons cat="ADVP"><tok cat="RB">exclusively</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">NFATp</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Western</tok> <tok cat="NN">blot</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBZ">shows</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="QP"><tok cat="RB">approximately</tok> <tok cat="CD">120</tok></cons> <tok cat="NN">kD</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@52@@oe@16-12-2010
765048610@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">do</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">express</tok> <cons cat="NP"><tok cat="NN">NFATc</tok></cons> <cons cat="ADVP"><tok cat="RB">constitutively</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">NFATc</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="NN">accumulation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">2</tok> <tok cat="NN">h</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">CD16</tok> <tok cat="NNS">ligands</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
765048611@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">supershift</tok> <tok cat="NNS">assays</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">available</tok> <cons cat="NP"><tok cat="NN">mAb</tok></cons></cons> <cons cat="VP"><tok cat="VBG">recognizing</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">NFATc</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="JJ">detectable</tok> <cons cat="NP"><tok cat="NN">NFATc</tok></cons> <tok cat="NN">protein</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">nuclear</tok> <cons cat="NP" ref="i10" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">cytoplasmic</tok> <cons cat="NP" ref="i10" null="RNR"></cons></cons><cons cat="NP" id="i10"><tok cat="NNS">extracts</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CD16-</tok> <cons cat="ADJP" null="QSTN"></cons><cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="JJ">ester-stimulated</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">any</tok> <tok cat="NN">time</tok></cons> <cons cat="VP"><tok cat="VBN">tested</tok><cons cat="NP" null="NONE"></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" func="ADV"><cons cat="NP"><cons cat="QP"><tok cat="RB">up</tok> <tok cat="TO">to</tok> <tok cat="CD">4</tok></cons> <tok cat="NN">h</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
765048612@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="JJ">direct</tok> <tok cat="NN">evidence</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="DT">both</tok> <cons cat="NP"><tok cat="JJ">CsA-sensitive</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NFATp</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NFATc</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP"><tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i13" role="SBJ"><tok cat="PRPP">their</tok> <cons cat="NP"><tok cat="NN">activation</tok></cons> <tok cat="CC">and/or</tok> <cons cat="NP"><tok cat="NN">expression</tok></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">single</tok> <tok cat="NN">stimulus</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i14"><tok cat="WDT">that</tok></cons><cons cat="S"><cons cat="NP" ref="i14" role="SBJ" null="T"></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">case</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD16</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFATp</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">subsequently</tok> <tok cat="VBN">induced</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFATc</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@66@@oe@16-12-2010
765418401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Relationship</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">Rap1</tok> <tok cat="NN">protein</tok></cons> <tok cat="NN">phosphorylation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Ca2+</tok> <tok cat="NN">transport</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">platelets</tok></cons></cons></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">new</tok> <tok cat="NN">approach</tok></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@16-12-2010
765418402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">interrelationship</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="NNS">messengers</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Ca2+</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">cyclic</tok> <tok cat="NN">AMP</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">platelet</tok> <tok cat="NN">function</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">well</tok></cons> <cons cat="VP"><tok cat="VBN">documented</tok><cons cat="NP" ref="i1" null="NONE"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">action</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">still</tok></cons> <cons cat="VP"><tok cat="VBZ">remains</tok> <cons cat="S"><cons cat="NP" ref="i2" id="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">established</tok><cons cat="NP" ref="i3" null="NONE"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@28@@oe@16-12-2010
765418403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">question</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">platelet</tok> <tok cat="NNS">Ca(2+)-ATPases</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">cyclic</tok> <tok cat="NN">AMP</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Rap1</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">pathological</tok> <tok cat="NN">model</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
765418404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">first</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">experimental</tok> <tok cat="NNS">conditions</tok></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i5"><tok cat="WRB">where</tok></cons> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">Ca(2+)-transport</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">platelet</tok> <tok cat="NN">membrane</tok> <tok cat="NNS">vesicles</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">appeared</tok> <cons cat="S"><cons cat="NP" ref="i6" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Rap1</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i5" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
765418405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Then</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i7" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">studied</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">platelets</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">congestive</tok> <tok cat="NN">heart</tok> <tok cat="NN">failure</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">potential</tok> <cons cat="NP"><tok cat="CD">97</tok> <tok cat="NN">kDa</tok> <tok cat="NN">Ca(2+)-ATPase</tok> <tok cat="NN">target</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">regulation</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Rap1</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Rap1</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i7" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">catalytic</tok> <tok cat="NN">subunit</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">cyclic</tok> <tok cat="JJ">AMP-dependent</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">C.</tok> <tok cat="NN">Sub.</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@16-12-2010
765418406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NNS">patients</tok></cons> <cons cat="VP"><tok cat="VBN">studied</tok><cons cat="NP" null="NONE"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="JJ">significant</tok> <tok cat="NN">modification</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="CD">97</tok> <tok cat="NN">kDa</tok> <tok cat="NN">Ca(2+)-ATPase</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Western</tok> <tok cat="NN">blotting</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">PL/IM</tok> <tok cat="CD">430</tok> <tok cat="JJ">monoclonal</tok> <tok cat="NN">antibody</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="VP"><tok cat="VBD">recognized</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">isoform</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
765418407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Rap1</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">differentially</tok></cons> <cons cat="VP"><tok cat="VBN">phosphorylated</tok> <cons cat="NP" ref="i9" null="NONE"></cons></cons><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i21"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">15</tok> <tok cat="NNS">micrograms/ml</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">C.</tok> <tok cat="NN">Sub</tok><tok cat="PERIOD">.</tok></cons></cons></cons></cons><cons cat="ADVP" ref="i21" null="T"></cons></cons></cons></cons></cons></cons>@@@@1@18@@oe@16-12-2010
765418408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBD">allowed</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="PRP">us</tok></cons> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">use</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="JJ">pathological</tok> <cons cat="NP"><tok cat="NNS">platelets</tok></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">study</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">relationship</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Rap1</tok> <tok cat="NN">protein</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Ca2+</tok> <tok cat="NN">transport</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i10" null="NONE"></cons><cons cat="VP"><tok cat="VBG">selecting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">patient</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">severe</tok> <tok cat="NN">heart</tok> <tok cat="NN">failure</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
765418409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">show</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok> <cons cat="PP" ref="i11" null="RNR"></cons></cons></cons><cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phosphorylation</tok> <cons cat="PP" ref="i11" null="RNR"></cons></cons></cons><cons cat="PP" id="i11"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Rap1</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">demonstrate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJR">lower</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">C.</tok> <tok cat="NN">Sub.</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">Ca2+</tok> <tok cat="NN">transport</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
765418410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">studying</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">further</tok> <tok cat="NN">series</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i12" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">confirm</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Rap1</tok> <tok cat="NN">protein</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">heart</tok> <tok cat="NN">failure</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="SBAR" func="ADV"><cons cat="WHNP"><tok cat="WDT">whatever</tok></cons> <cons cat="FRAG"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">extent</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">variable</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="ADVP"><tok cat="RB">strictly</tok></cons> <cons cat="VP"><tok cat="VB">correlate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Rap1</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">stimulatory</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">C.</tok> <tok cat="NN">Sub.</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">Ca2+</tok> <tok cat="NN">transport</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@16-12-2010
765418411@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Besides</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Rap1</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">platelets</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">heart</tok> <tok cat="NN">failure</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">constitute</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">new</tok> <tok cat="NN">approach</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">favour</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">platelet</tok> <tok cat="NN">Ca2+</tok> <tok cat="NN">transport</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Rap1</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
765716201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">enhancer</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">LEF-1</tok> <tok cat="NN">HMG</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nucleosome-assembled</tok> <tok cat="NN">DNA</tok></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@16-12-2010
765716202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Lymphoid</tok> <tok cat="JJ">enhancer-binding</tok> <tok cat="NN">factor</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LEF-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">regulatory</tok> <tok cat="JJ">high</tok> <tok cat="NN">mobility</tok> <tok cat="NN">group</tok> <tok cat="LRB">(</tok><tok cat="NN">HMG</tok><tok cat="RRB">)</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha</tok> <tok cat="LRB">(</tok><tok cat="NN">TCR</tok> <tok cat="NN">alpha</tok><tok cat="RRB">)</tok> <tok cat="NN">enhancer</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">context-restricted</tok> <tok cat="NN">manner</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@37@@oe@16-12-2010
765716203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">paper</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">distal</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus-1</tok> <tok cat="LRB">(</tok><tok cat="NN">HIV-1</tok><tok cat="RRB">)</tok> <tok cat="NN">enhancer</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">LEF-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Ets-1</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">provides</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">functional</tok> <tok cat="NN">context</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">LEF-1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
765716204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">First</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">mutations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">LEF-1-binding</tok> <tok cat="NN">site</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">multimerized</tok> <tok cat="NNS">copies</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">enhancer</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">LEF-1/GAL4</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">GAL4-substituted</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">enhancer</tok></cons></cons> <cons cat="NP" func="ADV"><cons cat="NP"><tok cat="CD">80-</tok> <cons cat="NP" null="QSTN"></cons></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="RB">100-fold</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
765716205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Second</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i3" role="SBJ"><tok cat="JJ">recombinant</tok> <tok cat="NN">LEF-1</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">chromatin-assembled</tok> <tok cat="NN">DNA</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
765716206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">nucleosome-assembly</tok> <tok cat="NN">system</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="FW">Drosophila</tok> <tok cat="NNS">embryos</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">find</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">packaging</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="NN">chromatin</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBZ">represses</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">repression</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">counteracted</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">efficiently</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">preincubation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">LEF-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><tok cat="CC">or</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">LEF-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Ets-1</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBN">supplemented</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">fractions</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">promoter-binding</tok> <tok cat="NN">protein</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Sp1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@55@@oe@16-12-2010
765716207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">TFE-3</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">E-box</tok> <tok cat="NN">motif</tok></cons> <cons cat="ADVP"><tok cat="JJ">upstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">LEF-1</tok> <cons cat="NP" ref="i7" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Ets-1</tok> <cons cat="NP" ref="i7" null="RNR"></cons></cons><cons cat="NP" id="i7"><tok cat="NNS">sites</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="ADVP"><tok cat="RB">further</tok></cons> <cons cat="VP"><tok cat="VBZ">augments</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">system</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
765716208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" syn="COOD"><cons cat="ADVP"><tok cat="RB">Individually</tok></cons> <tok cat="CC">or</tok> <cons cat="ADVP"><tok cat="RB">collectively</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">none</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">three</tok> <cons cat="NP"><tok cat="JJ">enhancer-binding</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">LEF-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Ets-1</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TFE-3</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">absence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Sp1</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
765716209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">A</tok> <cons cat="NP"><tok cat="NN">truncation</tok> <tok cat="NN">mutant</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">LEF-1</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">HMG-88</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HMG</tok> <tok cat="NN">box</tok></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBZ">lacks</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">trans-activation</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">nucleosomal</tok> <tok cat="NN">DNA</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">bending</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HMG</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">sufficient</tok></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@16-12-2010
765716210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">LEF-1</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">chromatin-dependent</tok> <tok cat="NN">process</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">functional</tok> <cons cat="NP"><tok cat="NN">trans-activation</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HMG</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
765764501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="NP"><tok cat="NN">NFATp</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">cyclosporin</tok> <tok cat="JJ">A-treated</tok> <tok cat="JJ">human</tok> <tok cat="NN">B</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@16-12-2010
765764502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="NN">Cyclosporin</tok> <tok cat="NN">A</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CsA</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">exerts</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">immunosuppressive</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inhibiting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NFAT</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="VP"><tok cat="VBG">preventing</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NN">cytokine</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@33@@oe@16-12-2010
765764503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">effect</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">thought</tok> <cons cat="S"><cons cat="NP" ref="i3" id="i4" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADVP"><tok cat="RB">largely</tok></cons> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">inactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phosphatase</tok> <cons cat="NP"><tok cat="NN">calcineurin</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">turn</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">NFAT</tok></cons> <tok cat="NN">component</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
765764504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">CsA</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Raji</tok> <tok cat="NN">B</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">yields</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">phosphorylated</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFATp</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" id="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">DNA-binding</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">form</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">NFAT</tok> <tok cat="NN">complex</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Fos</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Jun</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
765764505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Immunoblot</tok> <tok cat="NNS">analyses</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">metabolic</tok> <tok cat="NN">labeling</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">[32P]orthophosphate</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="NN">CsA</tok></cons> <cons cat="VP"><tok cat="VBZ">alters</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">NFATp</tok></cons> <tok cat="NN">migration</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">SDS-polyacrylamide</tok> <tok cat="NN">gel</tok> <tok cat="NN">electrophoresis</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">increasing</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">phosphorylation</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">affecting</tok> <cons cat="NP"><tok cat="JJ">subcellular</tok> <tok cat="NN">distribution</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
765764506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Dephosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">calcineurin</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">alkaline</tok> <tok cat="NN">phosphatase</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">restores</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">NFATp</tok></cons> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">reconstitute</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">NFAT</tok> <tok cat="NN">complex</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">Fos</tok></cons> <cons cat="NP" ref="i10" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Jun</tok></cons> <cons cat="NP" ref="i10" null="RNR"></cons></cons><cons cat="NP" id="i10"><tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
765764507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">point</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">new</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">CsA-sensitive</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NFATp</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i12"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">dephosphorylation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">critical</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">binding</tok></cons></cons><cons cat="PP" ref="i12" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
765782501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Constitutive</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">different</tok> <cons cat="NP"><tok cat="NN">Jak</tok> <tok cat="NN">tyrosine</tok> <tok cat="NNS">kinases</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok> <tok cat="LRB">(</tok><tok cat="NN">HTLV-1</tok><tok cat="RRB">)</tok> <tok cat="NN">tax</tok> <tok cat="NN">protein</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">virus-transformed</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@16-12-2010
765782502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HTLV-1</tok> <tok cat="NN">infection</tok></cons> <cons cat="VP"><tok cat="VBZ">causes</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">adult</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">leukemia</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">humans</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@11@@oe@16-12-2010
765782503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="VBN">encoded</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="NP"><tok cat="NN">tax</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">thought</tok> <cons cat="S"><cons cat="NP" ref="i1" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">oncogenesis</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
765782504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRPP">Our</tok> <tok cat="JJ">previous</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">tax</tok></cons> <tok cat="JJ">transgenic</tok> <tok cat="NN">mouse</tok> <tok cat="NN">model</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">tax</tok></cons> <cons cat="VP"><tok cat="VBZ">transforms</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">mouse</tok> <tok cat="NNS">fibroblasts</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NNS">thymocytes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">despite</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">comparable</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">tax</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">tissues</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
765782505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="JJ">Constitutive</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">130-kD</tok> <tok cat="NN">protein</tok></cons><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">tax</tok></cons> <cons cat="NP"><tok cat="JJ">transformed</tok> <tok cat="NN">fibroblast</tok> <tok cat="NN">B</tok> <tok cat="NN">line</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">HTLV-1</tok> <tok cat="JJ">transformed</tok> <tok cat="JJ">human</tok> <tok cat="JJ">lymphoid</tok> <tok cat="NNS">lines</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">thymocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">Thy-tax</tok> <tok cat="JJ">transgenic</tok> <tok cat="NNS">mice</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
765782506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Phosphotyrosine</tok> <tok cat="NN">immunoprecipitation</tok></cons> <cons cat="VP"><tok cat="VBN">followed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">Western</tok> <tok cat="NN">blot</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">set</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Jak</tok> <tok cat="NN">kinase</tok> <tok cat="JJ">specific</tok> <tok cat="NNS">antibodies</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><tok cat="NN">p130</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP" id="i30"><tok cat="NN">Jak2</tok></cons></cons> <cons cat="PP" id="i31"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">tax</tok></cons> <tok cat="JJ">transformed</tok> <cons cat="NP"><tok cat="NN">mouse</tok> <tok cat="JJ">fibroblastic</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><cons map="i30" cat="NP"><tok cat="NN">Jak3</tok></cons></cons> <cons map="i31" cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">HTLV-1</tok> <tok cat="JJ">transformed</tok> <tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
765782507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Jak2</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">tax</tok> <tok cat="JJ">transformed</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-6</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
765782508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="NN">Tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Balb/c3T3</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">supernatant</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">line</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" id="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">proliferation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
765782509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="CC">Both</tok> <cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">proliferation</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">IL-6</tok> <tok cat="NN">neutralizing</tok> <tok cat="NNS">antibodies</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
765782510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Constitutive</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Jak</tok> <tok cat="NNS">kinases</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">facilitate</tok> <cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">growth</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">HTLV-1</tok> <tok cat="JJ">infected</tok> <tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transgenic</tok> <tok cat="NN">mouse</tok> <tok cat="NN">model</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
765952901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">erythropoietin</tok> <tok cat="NN">receptor</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok> <tok cat="CC">and</tok> <tok cat="NN">Sp1</tok> <tok cat="NNS">motifs</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@16-12-2010
765952902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i15" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Erythropoietin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Epo</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">primary</tok> <tok cat="NN">regulator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">acts</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i15" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EpoR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NNS">progenitors</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@30@@oe@16-12-2010
765952903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">used</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">deletion</tok> <tok cat="NN">analysis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">transfection</tok> <tok cat="NNS">assays</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">reporter</tok> <tok cat="NN">gene</tok> <tok cat="NNS">constructs</tok></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">examine</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">control</tok> <tok cat="NNS">elements</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">5'</tok> <tok cat="JJ">flanking</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">EpoR</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
765952904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="JJS">most</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">contained</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="CD">150</tok> <tok cat="NN">bp</tok> <tok cat="NN">promoter</tok></cons> <tok cat="NN">fragment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">binding</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">AP2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Sp1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">erythroid-specific</tok> <tok cat="NN">GATA-1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
765952905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="CD">150</tok> <tok cat="NN">bp</tok> <tok cat="NN">hEpoR</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="VP"><tok cat="VBD">exhibited</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">high</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">low</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons><cons cat="NP" id="i3"><tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">erythroid</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">OCIM1</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">K562</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i5" null="NONE"></cons><cons cat="VP"><tok cat="VBG">reflecting</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">high</tok> <cons cat="NP" ref="i6" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">low</tok> <cons cat="NP" ref="i6" null="NONE"></cons></cons><cons cat="NP" id="i6"><tok cat="NNS">levels</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">constitutive</tok> <cons cat="NP"><tok cat="NN">hEpoR</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
765952906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok> <cons cat="NP" ref="i7" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Sp1</tok> <cons cat="NP" ref="i7" null="RNR"></cons></cons><cons cat="NP" id="i7"><tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">promoter</tok></cons> <cons cat="VP"><tok cat="VBG">lacking</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">TATA</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">necessary</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">high</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
765952907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Protein-DNA</tok> <tok cat="NN">binding</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBD">suggested</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Sp1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">other</tok> <cons cat="NP"><tok cat="NN">CCGCCC</tok> <tok cat="NN">binding</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">erythroid</tok></cons> <cons cat="NP" ref="i8" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">non-erythroid</tok></cons> <cons cat="NP" ref="i8" null="RNR"></cons></cons><cons cat="NP" id="i8"><tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Sp1</tok> <tok cat="NN">binding</tok> <tok cat="NN">motif</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
765952908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">increasing</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GATA-1</tok></cons> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="NN">co-transfection</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">transactivate</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">hEpoR</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">K562</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">non-erythroid</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">highly</tok> <tok cat="JJ">active</tok></cons> <cons cat="NP"><tok cat="NN">OCIM1</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">GATA-1</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">comparable</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">OCIM1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">K562</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
765952909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Interestingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i10"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">mutated</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Sp1</tok> <tok cat="NN">site</tok></cons></cons><cons cat="ADVP" ref="i10" null="T"></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">marked</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">hEpoR</tok> <tok cat="NN">promoter</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">restore</tok> <cons cat="NP"><tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">increasing</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GATA-1</tok></cons> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">OCIM1</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
765952910@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">GATA-1</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">transactivate</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">EpoR</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">hEpoR</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">depend</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GATA-1</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
765952911@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Rather</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="NN">hEpoR</tok></cons> <tok cat="NN">transcription</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBZ">depends</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">coordination</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Sp1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <cons cat="NP"><tok cat="JJ">cell-specific</tok> <tok cat="NNS">factors</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="ADVP"><tok cat="RB">possibly</tok></cons> <cons cat="NP"><tok cat="JJ">other</tok> <cons cat="NP"><tok cat="JJ">Sp1-like</tok> <tok cat="NN">binding</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">provide</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">high</tok> <tok cat="NN">level</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">tissue-specific</tok></cons></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
766298201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">TCL1</tok> <tok cat="NN">oncogene</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">preleukemic</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">case</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ataxia-telangiectasia</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
766298202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">TCL1</tok> <tok cat="NN">oncogene</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">chromosome</tok></cons> <cons cat="NP"><tok cat="NN">14q32.1</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="NN">chromosome</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">translocations</tok></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">t(14;14)(q11;q32.1)</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">t(7;14)(q35;q32.1)</tok></cons></cons><tok cat="RRB">]</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">inversions</tok></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP"><tok cat="NN">inv14(q11;q32.1)</tok></cons><tok cat="RRB">]</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">TCR</tok> <tok cat="NN">alpha/beta</tok> <tok cat="NNS">loci</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NNS">leukemias</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">T-prolymphocytic</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">T-PLL</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@37@@oe@16-12-2010
766298203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">T- acute</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons><tok cat="CC">and</tok><cons cat="NP"><cons cat="NP" null="QSTN"></cons><tok cat="JJ">- chronic</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons><cons cat="NP" id="i3"><tok cat="NNS">leukemias</tok></cons></cons> <cons cat="VP"><tok cat="VBG">arising</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cases</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">ataxia-telangiectasia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AT</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">syndrome</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
766298204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Similar</tok> <tok cat="JJ">chromosomal</tok> <tok cat="NNS">rearrangements</tok></cons> <cons cat="VP"><tok cat="VBP">occur</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">clonally</tok> <tok cat="VBN">expanded</tok></cons> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">AT</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">before</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">appearance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">overt</tok> <tok cat="NN">leukemia</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
766298205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">TCL1</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">PBLs</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="CD">four</tok> <tok cat="NN">AT</tok> <tok cat="NNS">cases</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">controls</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
766298206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TCL1</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">overexpressed</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NNS">PBLs</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">AT</tok> <tok cat="NN">patient</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">large</tok> <tok cat="JJ">clonal</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">population</tok></cons></cons> <cons cat="VP"><tok cat="VBG">exhibiting</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">t(14;14)</tok> <tok cat="NN">translocation</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">other</tok> <tok cat="NNS">cases</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
766298207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Fluorescence</tok> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">situ</tok></cons> <tok cat="NN">hybridization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TCL1</tok> <tok cat="JJ">genomic</tok> <tok cat="NN">locus</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lymphocyte</tok> <tok cat="NNS">metaphases</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">AT</tok> <tok cat="NN">patient</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">T-cell</tok> <tok cat="JJ">clonal</tok> <tok cat="NN">expansion</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">breakpoint</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">t(14;14)</tok> <tok cat="NN">translocation</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">lies</tok> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TCL1</tok> <tok cat="NN">locus</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">accompanied</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="VBN">inverted</tok> <tok cat="NN">duplication</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">distal</tok> <tok cat="NN">part</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="CD">14</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@49@@oe@16-12-2010
766298208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">TCL1</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">preleukemic</tok> <tok cat="JJ">clonal</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">consequence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="NN">translocation</tok></cons> <cons cat="VP"><tok cat="NN">involving</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">sequences</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TCR</tok> <tok cat="NN">locus</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="NN">14q11</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
766298209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Deregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TCL1</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NN">event</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">initiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">malignancy</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">types</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">leukemias</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">represents</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potential</tok> <tok cat="NN">tool</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">clinical</tok> <tok cat="NN">evaluation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
766352001@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Disruption</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">GATA</tok> <tok cat="NN">motif</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Duffy</tok> <tok cat="NN">gene</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">abolishes</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">Duffy-negative</tok> <tok cat="NNS">individuals</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@16-12-2010
766352002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" id="i1" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">mRNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Duffy</tok> <tok cat="NN">blood</tok> <tok cat="NN">group</tok> <tok cat="NN">antigen</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">erythrocyte</tok> <tok cat="NN">receptor</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Plasmodium</tok> <tok cat="NN">vivax</tok> <tok cat="NN">malaria</tok> <tok cat="NN">parasite</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">cloned</tok> <cons cat="NP" ref="i1" null="NONE"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">encode</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">widely</tok> <tok cat="VBN">expressed</tok></cons> <cons cat="NP"><tok cat="NN">chemokine</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@33@@oe@16-12-2010
766352003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Duffy</tok> <tok cat="NN">antigen/chemokine</tok> <tok cat="NN">receptor</tok> <tok cat="NN">gene</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">DARC</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">composed</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">single</tok> <cons cat="NP"><tok cat="NN">exon</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP"><tok cat="JJS">most</tok> <tok cat="JJ">Duffy-negative</tok></cons> <tok cat="NNS">blacks</tok></cons> <cons cat="VP"><tok cat="VBP">carry</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">silent</tok> <tok cat="NN">FY*B</tok> <tok cat="NN">allele</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">single</tok> <tok cat="NN">T</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">C</tok> <tok cat="NN">substitution</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="NN">nucleotide</tok> <tok cat="CD">-46</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
766352004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">mutation</tok></cons> <cons cat="VP"><tok cat="VBZ">impairs</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">disrupting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="VBG">binding</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GATA1</tok> <tok cat="JJ">erythroid</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
766352005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">With</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">recent</tok> <tok cat="NN">characterization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">FY*A</tok> <cons cat="NP" ref="i4" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">FY*B</tok> <cons cat="NP" ref="i4" null="RNR"></cons></cons><cons cat="NP" id="i4"><tok cat="NNS">alleles</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">basis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Duffy</tok> <tok cat="NN">blood</tok> <tok cat="NN">group</tok> <tok cat="NN">system</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">explanation</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">erythroid-specific</tok> <tok cat="NN">repression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">DARC</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">Duffy-negative</tok> <tok cat="NNS">individuals</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
766478101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Interleukin-2</tok> <tok cat="NN">promoter</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="JJ">virus-transformed</tok> <tok cat="NN">B</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">controlled</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-chi</tok> <tok cat="NN">B</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
766478102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="LRB">(</tok><tok cat="NN">IL</tok><tok cat="RRB">)</tok><tok cat="CD">-2</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><cons cat="ADVP"><tok cat="RB">mainly</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">predominant</tok> <tok cat="NNS">producers</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@16-12-2010
766478103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VB">synthesize</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
766478104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">analyzed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok> <tok cat="LRB">(</tok><tok cat="NN">EBV</tok><tok cat="RRB">)</tok><tok cat="JJ">-transformed</tok> <tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NNS">clones</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" id="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">secreting</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">low</tok> <tok cat="NN">level</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">12-myristate</tok> <tok cat="NN">13-acetate</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Ca2+</tok> <tok cat="NN">ionophore</tok> <tok cat="NN">ionomycin</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
766478105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="JJ">Transient</tok> <tok cat="NNS">transfections</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">reporter</tok> <tok cat="NNS">constructs</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">multiples</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">distal</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="LRB">(</tok><tok cat="NN">NF</tok><tok cat="RRB">)</tok><tok cat="NN">-AT</tok></cons><cons cat="NP" ref="i6" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">proximal</tok> <tok cat="NN">NF-AT</tok></cons><cons cat="NP" ref="i6" null="NONE"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">AP-1/Octamer</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">UPS</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" ref="i6" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">NF-chi</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TCEd</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" ref="i6" null="RNR"></cons></cons><cons cat="NP" id="i6"><tok cat="NNS">sites</tok></cons></cons><tok cat="RRB">]</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">performed</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
766478106@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">EBV-transformed</tok> <tok cat="NN">B</tok> <tok cat="NNS">clones</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">chi</tok> <tok cat="NN">B</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="VP"><tok cat="VBD">exerted</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJS">strongest</tok></cons> <cons cat="ADJP"><tok cat="JJ">inducible</tok></cons></cons> <tok cat="NN">activity</tok></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok> <tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="DT">no</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">only</tok> <tok cat="JJ">weak</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="VBN">compared</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
766478107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">An</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="VP"><tok cat="VBG">bearing</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">defective</tok> <cons cat="NP"><tok cat="NN">NF-chi</tok> <tok cat="NN">B</tok> <tok cat="NN">site</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">completely</tok> <tok cat="JJ">inactive</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">EBV-transformed</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="ADVP"><tok cat="RB">still</tok></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
766478108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CD">seven</tok> <cons cat="NP"><tok cat="NN">EBV-B</tok> <tok cat="NN">cell</tok> <tok cat="NNS">clones</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><tok cat="NNS">lines</tok></cons></cons> <cons cat="VP"><tok cat="VBG">differing</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">capacity</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">secrete</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">correlated</tok> <cons cat="ADVP"><tok cat="RB">well</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">status</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="NN">secretion</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
766478109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Similarly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">promoter</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WPP">whose</tok> <tok cat="NN">activity</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" id="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">controlled</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="NN">chi</tok> <tok cat="NN">B</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i8" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" syn="COOD" role="PRD"><cons cat="ADJP"><tok cat="JJ">active</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">producing</tok> <tok cat="NN">EBV-B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="ADJP"><tok cat="JJ">inactive</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">non-IL-2-producing</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
766478110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">EBV-B</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons> <cons cat="NP"><tok cat="NN">NF-chi</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">probe</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">constitutive</tok> <tok cat="NN">generation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">chi</tok> <tok cat="NN">B</tok> <tok cat="NNS">complexes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2-secreting</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">consisting</tok> <cons cat="ADVP"><tok cat="RB">mainly</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">heterodimeric</tok> <tok cat="NN">p50/p65</tok> <tok cat="NNS">complexes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
766478111@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJR">weaker</tok> <cons cat="NP"><tok cat="NN">chi</tok> <tok cat="NN">B</tok></cons> <tok cat="JJ">complex</tok> <tok cat="NN">formation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">faster-migrating</tok> <tok cat="NNS">complexes</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">non-IL-2-secreting</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
766478112@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">NF-chi</tok> <tok cat="NN">B</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">indispensable</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">EBV-transformed</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="JJ">other</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="VP"><tok cat="VBP">appear</tok> <cons cat="S"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="RBR">less</tok> <tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
766556701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Transcriptional</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">gene</tok></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">C-type</tok> <tok cat="NN">lectin</tok> <tok cat="NN">leukocyte</tok> <tok cat="NN">receptor</tok></cons></cons> <cons cat="NP"><tok cat="NN">AIM/CD69</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="NN">characterization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="JJ">factor-alpha-responsive</tok> <tok cat="NNS">elements</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@16-12-2010
766556702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">activation</tok> <tok cat="NN">antigen</tok></cons> <cons cat="NP"><tok cat="NN">CD69</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">member</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">C-type</tok> <tok cat="NN">animal</tok> <tok cat="NN">lectin</tok> <tok cat="NN">superfamily</tok></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">functions</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">signal-transmitting</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@16-12-2010
766556703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD69</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJS">most</tok> <tok cat="JJ">hematopoietic</tok> <tok cat="NNS">lineages</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">wide</tok> <tok cat="NN">variety</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">stimuli</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">mainly</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">T-lymphocytes</tok></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inflammatory</tok> <tok cat="NNS">infiltrates</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">several</tok> <tok cat="JJ">human</tok> <tok cat="NNS">diseases</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
766556704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">elucidate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">regulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">constitutive</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">inducible</tok></cons></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD69</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NNS">leukocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">isolated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CD69</tok></cons> <tok cat="NN">gene</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">carried</tok> <cons cat="PRT"><tok cat="RP">out</tok></cons> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">functional</tok> <tok cat="NN">characterization</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
766556705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Sequence</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">5'-flanking</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CD69</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potential</tok> <cons cat="NP"><tok cat="NN">TATA</tok> <tok cat="NN">element</tok></cons></cons> <cons cat="ADVP"><cons cat="ADVP"><cons cat="NP"><tok cat="CD">30</tok> <tok cat="NN">base</tok> <tok cat="NNS">pairs</tok> <tok cat="JJ">upstream</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">major</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">initiation</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">several</tok> <tok cat="JJ">putative</tok> <tok cat="NN">binding</tok></cons> <tok cat="NNS">sequences</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">inducible</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Egr-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">mediate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inducible</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@56@@oe@16-12-2010
766556706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Transient</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD69</tok> <tok cat="JJ">promoter-based</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok> <tok cat="NNS">constructs</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">K562</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">indicated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">proximal</tok> <tok cat="NN">promoter</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="VP"><tok cat="VBG">spanning</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">positions</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="CD">-78</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="CD">+16</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">cis-acting</tok> <tok cat="NNS">sequences</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">necessary</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">basal</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="NN">phorbol</tok> <tok cat="NN">12-myristate</tok> <tok cat="JJ">13-acetate-inducible</tok></cons></cons> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CD69</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
766556707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Removal</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">upstream</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">positions</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-78</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-38</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">decreased</tok> <tok cat="NN">promoter</tok> <tok cat="NN">strength</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">abolished</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">12-myristate</tok> <tok cat="NN">13-acetate</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
766556708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor-alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inducing</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">surface</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CD69</tok></cons> <tok cat="NN">molecule</tok></cons></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">fusion</tok> <tok cat="NNS">plasmids</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">contain</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">5'-flanking</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CD69</tok></cons> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">cytokine</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD69</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@52@@oe@16-12-2010
766556709@GENIA Treebank@formal@@1@S@<cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">ABSTRACT</tok></cons> <cons cat="VP"><tok cat="VBN">TRUNCATED</tok> <cons cat="PP"><tok cat="IN">AT</tok> <cons cat="NP"><tok cat="CD">250</tok> <tok cat="NNS">WORDS</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons>@@@@1@7@@oe@16-12-2010
766558801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Ubiquitin-mediated</tok> <tok cat="NN">processing</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activator</tok> <tok cat="NN">precursor</tok></cons></cons> <cons cat="NP"><tok cat="NN">p105</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@10@@oe@16-12-2010
766558802@GENIA Treebank@formal@@1@S@<cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">Reconstitution</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">cell-free</tok> <tok cat="NN">system</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">identification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">ubiquitin-carrier</tok> <tok cat="NN">protein</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">E2</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <cons cat="NP"><tok cat="JJ">ubiquitin-protein</tok> <tok cat="NN">ligase</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">E3</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">conjugation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
766558803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJS">most</tok> <tok cat="NNS">cases</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">heterodimer</tok></cons></cons> <cons cat="VP"><tok cat="VBG">consisting</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">subunits</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p50</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" id="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">encoded</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">distinct</tok> <tok cat="NNS">genes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Rel</tok> <tok cat="NN">family</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@33@@oe@16-12-2010
766558804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="NN">p50</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">translated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">precursor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">105</tok> <tok cat="NN">kDa</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
766558805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">C-terminal</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">precursor</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok></cons> <cons cat="VP"><tok cat="VBN">degraded</tok><cons cat="NP" ref="i4" null="NONE"></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">forming</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="NN">p50</tok> <tok cat="NN">subunit</tok></cons></cons> <cons cat="VP"><tok cat="VBN">consisted</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">N-terminal</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">molecule</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
766558806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">generation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p50</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">known</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
766558807@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">suggested</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">ubiquitin-proteasome</tok> <tok cat="NN">system</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">process</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">specific</tok> <cons cat="NP"><tok cat="NNS">enzymes</tok></cons></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">limited</tok> <tok cat="NN">proteolysis</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHPP" id="i8"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">half</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">molecule</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">spared</tok><cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP" ref="i8" null="T"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">obscure</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
766558808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNP">Palombella</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">colleagues</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNP">Palombella</tok><tok cat="COMMA">,</tok> <tok cat="NNP">V.J.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Rando</tok><tok cat="COMMA">,</tok> <tok cat="NNP">O.J.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Goldberg</tok><tok cat="COMMA">,</tok> <tok cat="NNP">A.L.</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNP">Maniatis</tok><tok cat="COMMA">,</tok> <tok cat="NNP">T.</tok></cons></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">1994</tok></cons><tok cat="RRB">)</tok></cons> <cons cat="NP"><tok cat="NNP">Cell</tok> <tok cat="CD">78</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">773-785</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="NN">ubiquitin</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">processing</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">cell-free</tok> <tok cat="NN">system</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="VBN">truncated</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="RB">artificially</tok> <tok cat="VBN">constructed</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">60-kDa</tok> <tok cat="NN">precursor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@16-12-2010
766558809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">They</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">proteasome</tok></cons> <tok cat="NNS">inhibitors</tok></cons> <cons cat="VP"><tok cat="VBP">block</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">processing</tok></cons> <cons cat="ADVP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
766558810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">reconstitution</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">cell-free</tok> <tok cat="NN">processing</tok> <tok cat="NN">system</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="ADVP"><tok cat="RB">directly</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok><cons cat="S" syn="COOD"><tok cat="COLON">:</tok> <cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">a</tok><tok cat="RRB">)</tok></cons> <cons cat="NP" id="i11" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">ubiquitin-proteasome</tok> <tok cat="NN">system</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">processing</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">intact</tok> <cons cat="NP"><tok cat="NN">p105</tok></cons> <tok cat="NN">precursor</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">b</tok><tok cat="RRB">)</tok></cons> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">conjugation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ubiquitin</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">precursor</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">an</tok> <tok cat="JJ">essential</tok> <tok cat="JJ">intermediate</tok> <tok cat="NN">step</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">processing</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">c</tok><tok cat="RRB">)</tok></cons> <cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">recently</tok> <tok cat="VBN">discovered</tok> <tok cat="JJ">novel</tok></cons> <tok cat="NNS">species</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">ubiquitin-carrier</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">E2-F1</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i13"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i13" id="i14" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i14" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">conjugation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">degradation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p53</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">limited</tok> <tok cat="NN">processing</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">p105</tok> <tok cat="NN">precursor</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">d</tok><tok cat="RRB">)</tok></cons> <cons cat="NP" id="i15" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok><tok cat="COMMA">,</tok> <tok cat="RB">approximately</tok> <cons cat="NP"><tok cat="CD">320-kDa</tok> <tok cat="NNS">species</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">ubiquitin-protein</tok> <tok cat="NN">ligase</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">process</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@109@@oe@16-12-2010
766558811@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="JJ">novel</tok> <tok cat="NN">enzyme</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">distinct</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">E6-AP</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">p53-conjugating</tok> <tok cat="NN">ligase</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">E3</tok> <tok cat="NN">alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="LQT">"</tok><tok cat="JJ">N-end</tok> <tok cat="NN">rule</tok><tok cat="RQT">"</tok> <tok cat="NN">ligase</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
766838501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Nonradioactive</tok> <tok cat="NN">quantification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">U937</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@16-12-2010
766838502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">describe</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">method</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">relative</tok> <tok cat="NN">quantification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <cons cat="NP"><tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">nonradioactive</tok> <tok cat="NN">assay</tok></cons> <cons cat="PP"><tok cat="VBN">based</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">strand</tok> <tok cat="NN">competition</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">identical</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">biotin-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">fluorescein-labeled</tok></cons></cons> <tok cat="NN">amplicon</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">probe</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">unlabeled</tok> <tok cat="NN">amplicon</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">target</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">hybridization</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@39@@oe@16-12-2010
766838503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">As</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">target</tok> <tok cat="NN">quantity</tok></cons> <cons cat="VP"><tok cat="VBD">increased</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">double-labeled</tok> <tok cat="NN">probe</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">decreased</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">accordance</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mass</tok> <tok cat="NN">action</tok> <tok cat="NN">law</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
766838504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">technique</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">successfully</tok></cons> <cons cat="VP"><tok cat="VBN">applied</tok> <cons cat="NP" ref="i1" null="NONE"></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">evaluate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">differences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PP" syn="COOD" adv="TMP"><cons cat="PP"><tok cat="IN">before</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">after</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><cons cat="NP" id="i2"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">potent</tok> <tok cat="NN">differentiation</tok> <tok cat="NNS">inducers</tok><cons cat="NP" ref="i11" null="ICH"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD" id="i11"><cons cat="NP"><cons cat="NP"><tok cat="NN">12-O-tetradecanoylphorbol</tok> <tok cat="NN">13-acetate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TPA</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">all-trans</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">1,25-dihydroxyvitamin</tok> <tok cat="NN">D2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">VD</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@16-12-2010
766838505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="NN">TPA</tok> <tok cat="NN">treatment</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">[3H]dexamethasone</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">up-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GR</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="DT">no</tok> <tok cat="VBN">enhanced</tok> <cons cat="NP"><tok cat="NN">GR</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">perceived</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">RA</tok><tok cat="NN">/VD</tok> <tok cat="NN">treatment</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
767011401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="NP"><tok cat="NN">PU.1</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">downregulated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">multiple</tok> <tok cat="NN">myeloma</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@16-12-2010
767011402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PU.1</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="VP"><tok cat="VBG">belonging</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Ets-family</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@11@@oe@16-12-2010
767011403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">identical</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Spi-1</tok> <tok cat="NN">oncogene</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" id="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">spleen</tok> <tok cat="JJ">focus-forming</tok></cons> <cons cat="ADJP"><tok cat="JJ">virus-induced</tok></cons> <cons cat="ADJP"><tok cat="JJ">murine</tok></cons></cons> <tok cat="NNS">erythroleukemias</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
767011404@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="NN">PU.1</tok></cons> <cons cat="VP"><tok cat="VBZ">seems</tok> <cons cat="S"><cons cat="NP" ref="i4" id="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">development</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">multiple</tok> <tok cat="JJ">hematopoietic</tok> <tok cat="NNS">lineages</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">preferentially</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">B-cell-and</tok> <tok cat="NN">monocyte/macrophage-differentiation</tok> <tok cat="NN">lineage</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
767011405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">so-called</tok> <cons cat="NP"><tok cat="NN">Pu</tok> <tok cat="NN">box</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <cons cat="NP"><tok cat="JJ">tissue-specific</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">DNA</tok> <tok cat="NN">element</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NNS">lineages</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
767011406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">expression</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PU.1</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B-cell</tok> <tok cat="NN">development</tok></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">panel</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBG">representing</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">stages</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">maturation</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NNS">precursors</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="VBN">differentiated</tok> <tok cat="NN">plasma</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
767011407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">PU.1</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="NN">expression</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PU.1</tok></cons> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">measured</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">Northern</tok> <tok cat="NN">blot</tok> <tok cat="NN">analysis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">evident</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="VP"><tok cat="VBG">representing</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">pro-B</tok><cons cat="NP" ref="i8" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">pre-B</tok><cons cat="NP" ref="i8" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="NN">B</tok> <cons cat="NP" ref="i8" null="RNR"></cons></cons><cons cat="NP" id="i8"><tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
767011408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VB">show</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Pu</tok> <tok cat="JJ">box-dependent</tok> <tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NNS">transfections</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
767011409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">multiple</tok> <tok cat="NN">myeloma</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">representing</tok> <cons cat="NP"><tok cat="VBN">differentiated</tok><tok cat="COMMA">,</tok> <tok cat="NN">plasma</tok> <tok cat="JJ">cell-like</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">PU.1</tok></cons> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Pu</tok> <tok cat="JJ">box-dependent</tok> <tok cat="NN">transactivation</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="JJ">absent</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">detectable</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">very</tok> <tok cat="JJ">low</tok></cons> <tok cat="NN">level</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
767011410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">lymphoblastoid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">exemplify</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">intermediate</tok> <tok cat="NN">stage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><tok cat="DT">a</tok> <tok cat="VBN">reduced</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">expression</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">activity</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">observed</tok><cons cat="NP" ref="i10" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
767011411@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">findings</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">multiple</tok> <tok cat="NN">myeloma</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">represent</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NNS">examples</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="VBN">downregulated</tok> <cons cat="NP"><tok cat="NN">PU.1</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">apparently</tok></cons> <cons cat="VP"><tok cat="VBP">contradict</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">observations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">murine</tok> <tok cat="NN">system</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHPP" id="i11"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="NN">PU.1</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="UCP" syn="COOD"><cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i12" null="NONE"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">active</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">plasmacytoma</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons><cons cat="PP" ref="i11" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
767011412@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">At</tok> <cons cat="NP"><tok cat="JJ">present</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRP">it</tok></cons> <cons cat="SBAR" ref="i13" null="EXP"></cons></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unclear</tok></cons> <cons cat="SBAR" id="i13"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">lack</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PU.1</tok></cons> <cons cat="NP"><tok cat="NN">expression</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">activity</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">multiple</tok> <tok cat="NN">myeloma</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">represents</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">malignancy-associated</tok> <tok cat="NN">defect</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="VP"><tok cat="VBZ">exemplifies</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">normal</tok> <tok cat="JJ">developmental</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="RB">terminally</tok> <tok cat="VBN">differentiated</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
767319401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">Microtubules</tok></cons> <cons cat="VP"><tok cat="VBP">mediate</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NN">25-hydroxyvitamin</tok> <tok cat="NN">D3</tok> <tok cat="NN">trafficking</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">genomic</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">1,25-dihydroxyvitamin</tok> <tok cat="NN">D3</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@16-12-2010
767319402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">genomic</tok> <tok cat="NNS">actions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">1,25-dihydroxyvitamin</tok> <tok cat="NN">D3</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">1,25(OH)2D3</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="JJ">intracellular</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">vitamin</tok> <tok cat="NN">D</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">VDR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@16-12-2010
767319403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">immunocytochemistry</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">disruption</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">microtubular</tok> <tok cat="NN">assembly</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">prevents</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">access</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">sterol-VDR</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">microtubules</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">1,25(OH)2D3</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">studied</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">viable</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
767319404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
767319405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">Monocytes</tok></cons> <cons cat="VP"><tok cat="VBP">convert</tok> <cons cat="NP"><tok cat="NN">25(OH)D3</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">1,25(OH)2D3</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">24-hydroxylated</tok> <tok cat="NNS">metabolites</tok></cons> <cons cat="ADJP"><cons cat="ADJP"><tok cat="RBR">more</tok> <tok cat="JJ">polar</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><tok cat="NN">1,25(OH)2D3</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
767319406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Microtubule</tok> <tok cat="NN">disruption</tok></cons> <cons cat="ADVP"><tok cat="RB">totally</tok></cons> <cons cat="VP"><tok cat="VBD">abolished</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">exogenous</tok> <tok cat="NN">1,25(OH)2D3</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">suppress</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">own</tok> <tok cat="NN">synthesis</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">24-hydroxylase</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">affecting</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="JJ">total</tok> <tok cat="NN">1,25(OH)2D3</tok> <tok cat="NN">uptake</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">maximal</tok> <tok cat="JJ">1,25(OH)2D3-VDR</tok> <tok cat="NN">binding</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
767319407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="JJ">intact</tok> <cons cat="NP"><tok cat="NNS">microtubules</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">1,25(OH)2D3-dependent</tok> <tok cat="NN">modulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
767319408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Interestingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="NN">microtubule</tok></cons> <tok cat="NN">disruption</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">decreased</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">monocyte</tok></cons> <tok cat="NN">1,25(OH)2D3</tok> <tok cat="NN">synthesis</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP" syn="COOD"><tok cat="RB">not</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">decreasing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Vmax</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">monocyte</tok> <tok cat="JJ">mitochondrial</tok> <tok cat="CD">1</tok> <tok cat="NN">alpha-hydroxylase</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Km</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">25(OH)2D3</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
767319409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><tok cat="NN">25(OH)D3</tok> <tok cat="NN">transport</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@5@@oe@16-12-2010
767319410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Microtubule</tok> <tok cat="NN">disruption</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><tok cat="JJ">total</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">25(OH)D3</tok> <tok cat="NN">uptake</tok></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">reduced</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">intracellular</tok> <tok cat="NN">trafficking</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mitochondria</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
767319411@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">microtubules</tok></cons> <cons cat="VP"><tok cat="VBP">participate</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">25(OH)D3</tok> <tok cat="NN">transport</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">their</tok> <tok cat="NN">integrity</tok></cons> <cons cat="VP"><tok cat="VBZ">determines</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">1,25(OH)2D3</tok> <tok cat="NN">synthesis</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
767324001@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <cons cat="NP"><tok cat="JJ">regulatory</tok> <tok cat="NN">element</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">interleukin</tok> <tok cat="CD">2</tok> <tok cat="NN">gene</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="VBG">binding</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">zinc</tok> <tok cat="NN">finger</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Sp1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">EGR-1</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@16-12-2010
767324002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="CD">2</tok> <tok cat="LRB">(</tok><tok cat="NN">IL-2</tok><tok cat="RRB">)</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">antigen</tok> <tok cat="NN">recognition</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <tok cat="JJ">critical</tok> <tok cat="NN">event</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <cons cat="NP"><tok cat="NN">proliferation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">effector</tok> <tok cat="NN">function</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@16-12-2010
767324003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Prior</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">stimulated</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
767324004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">describe</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <cons cat="NP"><tok cat="JJ">regulatory</tok> <tok cat="NN">element</tok></cons></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="ADVP"><tok cat="RB">immediately</tok> <tok cat="RB">upstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="IN">of</tok> <tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="LRB">(</tok><tok cat="NN">NFAT</tok><tok cat="RRB">)</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
767324005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">This</tok> <tok cat="NN">region</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="VP"><tok cat="VBN">termed</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">zinc</tok> <tok cat="NN">finger</tok> <tok cat="NN">protein</tok> <tok cat="NN">binding</tok> <tok cat="NN">region</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ZIP</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">serves</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">binding</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="CD">two</tok> <cons cat="ADJP"><tok cat="RB">differently</tok> <tok cat="VBN">regulated</tok></cons> <cons cat="NP"><tok cat="NN">zinc</tok> <tok cat="NN">finger</tok> <tok cat="NNS">proteins</tok></cons><cons cat="NP" ref="i2" null="ICH"></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD" id="i2"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="RB">constitutively</tok> <tok cat="VBN">expressed</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="NP"><tok cat="NN">Sp1</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">inducible</tok> <tok cat="JJ">early</tok> <tok cat="NN">growth</tok> <tok cat="NN">response</tok> <tok cat="NN">protein</tok></cons> <cons cat="NP"><tok cat="NN">EGR-1</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
767324006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">unstimulated</tok> <tok cat="NNS">cells</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">do</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">secrete</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <cons cat="NP"><tok cat="NN">Sp1</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">region</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">stimulated</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">secreting</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">inducible</tok> <tok cat="NN">EGR-1</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">recognizes</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
767324007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">ZIP</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">serves</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">activator</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">ZIP</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NFAT</tok> <tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">maximal</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
767324008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">critical</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">ZIP</tok> <tok cat="NN">site</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
767877901@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">zinc</tok> <tok cat="NN">finger</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">Egr-1</tok></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" ref="i55" null="RNR"></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">restricts</tok> <cons cat="NP" ref="i55" null="RNR"></cons></cons></cons><cons cat="NP" id="i55"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">along</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">macrophage</tok> <tok cat="NN">lineage</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
767877902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cDNA</tok> <tok cat="NNS">clones</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NN">differentiation</tok> <tok cat="JJ">primary</tok> <tok cat="NN">response</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MyD</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">genes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">absence</tok> <tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">de</tok> <tok cat="FW">novo</tok></cons> <tok cat="NN">protein</tok> <tok cat="NN">synthesis</tok></cons> <cons cat="PP" adv="TMP"><tok cat="VBG">following</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">along</tok> <cons cat="NP"><cons cat="NP"><tok cat="CC">either</tok> <tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">macrophage</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">granulocyte</tok></cons></cons> <tok cat="NN">lineage</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">myeloblastic</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">HL-60</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
767877903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">One</tok> <tok cat="NN">cDNA</tok> <tok cat="NN">clone</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">primary</tok> <tok cat="NN">response</tok> <tok cat="NN">gene</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">upon</tok> <cons cat="NP"><tok cat="NN">macrophage</tok> <tok cat="NN">differentiation</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">encoded</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Egr-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">zinc</tok> <tok cat="NN">finger</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
767877904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i56" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">Egr-1</tok> <tok cat="NN">gene</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="S"><cons cat="NP" ref="i56" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADVP"><tok cat="RB">transcriptionally</tok></cons> <cons cat="ADJP" id="i102" role="PRD"><tok cat="JJ">silent</tok></cons> <cons cat="PP" id="i103"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">HL-60</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><cons map="i102" cat="ADJP" role="PRD"><tok cat="JJ">active</tok></cons> <cons map="i103" cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">U-937</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">M1</tok></cons></cons> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">latter</tok> <tok cat="CD">two</tok></cons> <cons cat="VP"><tok cat="VBG">being</tok> <cons cat="ADJP" role="PRD"><tok cat="VBN">predetermined</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">macrophage</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
767877905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Egr-1</tok> <tok cat="JJ">antisense</tok> <tok cat="NNS">oligomers</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">culture</tok> <tok cat="NNS">media</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">blocked</tok> <cons cat="NP"><tok cat="NN">macrophage</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="DT">both</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NNS">myeloblasts</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
767877906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i58" role="SBJ"><cons cat="NP"><tok cat="NN">HL-60</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <tok cat="VBG">expressing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">Egr-1</tok> <tok cat="NN">transgene</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HL-60Egr-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i58" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">macrophage</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">granulocyte</tok></cons><tok cat="COMMA">,</tok></cons> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
767877907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">observations</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Egr-1</tok></cons></cons></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" ref="i59" null="RNR"></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">restricts</tok> <cons cat="NP" ref="i59" null="RNR"></cons></cons></cons><cons cat="NP" id="i59"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">myeloblasts</tok></cons></cons> <cons cat="PP"><tok cat="IN">along</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">macrophage</tok> <tok cat="NN">lineage</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
767899401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><cons cat="NP"><tok cat="NN">tal-1</tok></cons> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">GATA-binding</tok></cons></cons> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">hematopoiesis</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
767899402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i61" role="SBJ"><tok cat="NN">Tal-1</tok> <tok cat="NNS">rearrangements</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i61" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="RB">nearly</tok> <tok cat="CD">30</tok></cons><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="JJ">acute</tok> <tok cat="JJ">lymphoblastic</tok> <tok cat="NN">leukemia</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
767899403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i62" role="SBJ"><tok cat="NN">Tal-1</tok> <tok cat="NN">gene</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">encodes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">putative</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">basic</tok> <tok cat="JJ">helix-loop-helix</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" ref="i62" id="i63" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADVP"><tok cat="RB">predominantly</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i63" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
767899404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pattern</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tal-1</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">purified</tok> <tok cat="JJ">human</tok> <tok cat="JJ">hematopoietic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">situ</tok></cons> <tok cat="NN">hybridization</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">reverse</tok> <tok cat="NN">transcriptase</tok> <tok cat="NN">polymerase</tok> <tok cat="NN">chain</tok> <tok cat="NN">reaction</tok> <tok cat="NN">analysis</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
767899405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">Both</tok> <tok cat="NNS">methods</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i64" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">tal-1</tok> <tok cat="NN">gene</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i64" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">megakaryocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">erythroblasts</tok></cons></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">basophilic</tok> <tok cat="NNS">granulocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
767899406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">tal-1</tok> <tok cat="NN">1A</tok> <tok cat="NN">promoter</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i65"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i65" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><tok cat="CD">two</tok> <tok cat="NN">consensus</tok> <tok cat="JJ">GATA-binding</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">active</tok></cons> <cons cat="PP"><tok cat="RB">mainly</tok> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">lineages</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
767899407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">Because</tok> <cons cat="S"><cons cat="NP" id="i66" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">GATA-1</tok> <tok cat="NN">gene</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" ref="i66" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">transactivate</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NNS">genes</tok></cons> <cons cat="ADJP"><tok cat="JJ">specific</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">erythroid</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">megakaryocytic</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">mastocytic/basophilic</tok></cons></cons> <tok cat="NNS">lineages</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">studied</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GATA-1</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="VBN">purified</tok> <tok cat="JJ">hematopoietic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
767899408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i67" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">GATA-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">tal-1</tok></cons></cons> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">coexpressed</tok> <cons cat="NP" ref="i67" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="CD">three</tok> <tok cat="NNS">lineages</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
767899409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Remarkably</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i68" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">downmodulated</tok> <cons cat="NP" ref="i68" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">erythroid</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">megakaryocytic</tok></cons></cons> <tok cat="JJ">terminal</tok> <tok cat="NN">maturation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
767899410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">immature</tok> <tok cat="JJ">hematopoietic</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i69" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">tal-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok></cons></cons> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">coexpressed</tok> <cons cat="NP" ref="i69" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">committed</tok> <tok cat="NNS">progenitors</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">CD34+/CD38(2+)</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">detectable</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RBS">most</tok> <tok cat="JJ">primitive</tok></cons> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">CD34(2+)/CD38-</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
767899411@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i70" role="SBJ"><tok cat="NN">GATA-2</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i70" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RBS">most</tok> <tok cat="JJ">primitive</tok></cons> <cons cat="NP" ref="i101" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="VBN">committed</tok> <tok cat="NNS">progenitors</tok> <cons cat="NP" ref="i101" null="RNR"></cons></cons></cons><cons cat="NP" id="i101"><tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" id="i72" role="SBJ"><tok cat="NN">GATA-3</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">mostly</tok></cons> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i72" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RBS">most</tok> <tok cat="JJ">primitive</tok></cons> <tok cat="NNS">ones</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
767899412@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Altogether</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">results</tok></cons> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">GATA-1</tok></cons> <cons cat="VP"><tok cat="VBZ">modulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">tal-1</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">erythroid</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">megakaryocytic</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">basosophilic</tok></cons></cons> <tok cat="NNS">lineages</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
768065301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><cons cat="NP"><tok cat="NN">Cell</tok> <tok cat="NN">type-</tok></cons> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">stage-specific</tok></cons></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CD20/B1</tok> <tok cat="NN">antigen</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">correlates</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">diverged</tok> <tok cat="NN">octamer</tok> <tok cat="NN">DNA</tok> <tok cat="NN">motif</tok></cons> <cons cat="ADJP"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
768065302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">CD20</tok><tok cat="LRB">(</tok><tok cat="NN">B1</tok><tok cat="RRB">)</tok> <tok cat="NN">gene</tok></cons> <cons cat="VP"><tok cat="VBZ">encodes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="NN">B</tok> <tok cat="JJ">cell-specific</tok></cons> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NN">cell</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">proliferation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
768065303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Studies</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">5'</tok> <tok cat="NN">deletion</tok> <tok cat="NN">CD20</tok> <tok cat="NN">promoter-CAT</tok> <tok cat="NNS">constructs</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <tok cat="VBN">revealed</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">regions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="NNS">bases</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-186</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-280</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="NNS">bases</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-280</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-454</tok></cons></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i43"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i43" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP"><tok cat="JJ">positive</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">elements</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
768065304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">sequence</tok> <tok cat="NN">element</tok></cons> <cons cat="ADJP"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RBS">most</tok> <tok cat="JJ">proximal</tok></cons> <tok cat="NN">region</tok></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="NNS">bases</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-214</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-201</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">TTCTTCTAATTAA</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i44"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i44" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">high</tok> <tok cat="JJ">constitutive</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD20</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD20</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">pre-B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@16-12-2010
768065305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i45" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">sequence</tok> <tok cat="NN">element</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">referred</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" ref="i45" null="NONE"></cons></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">BAT</tok> <tok cat="NN">box</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">its</tok> <tok cat="NN">deletion</tok></cons> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP"><tok cat="VBD">reduced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">CD20</tok> <tok cat="JJ">promoter-CAT</tok> <tok cat="NN">construct</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
768065306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">various</tok> <tok cat="JJ">mutant</tok> <tok cat="NNS">probes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">core</tok> <tok cat="NN">sequence</tok> <tok cat="NN">TAAT</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
768065307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Cross</tok> <tok cat="NN">competition</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">octamer</tok> <tok cat="NN">sequence</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Ig</tok> <tok cat="JJ">heavy</tok> <tok cat="NN">chain</tok> <tok cat="NN">promoter</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">BAT</tok> <tok cat="NN">box</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">TA-rich</tok> <tok cat="NN">sequence</tok></cons> <cons cat="ADJP"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CD21</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">all</tok> <tok cat="CD">three</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="VP"><tok cat="VBD">bound</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">BAT</tok> <tok cat="NN">box</tok> <tok cat="NN">binding</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="NP" syn="COOD" role="PRD"><cons cat="NP"><tok cat="NN">Oct-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Oct-2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@49@@oe@16-12-2010
768065308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Southwestern</tok> <tok cat="NN">blotting</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">UV</tok> <tok cat="NN">cross-linking</tok> <tok cat="NNS">studies</tok></cons></cons> <cons cat="VP"><tok cat="VBD">confirmed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">BAT</tok> <tok cat="NN">box</tok> <tok cat="NN">binding</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="NP" syn="COOD" role="PRD"><cons cat="NP"><tok cat="NN">Oct-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Oct-2</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
768065309@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">affinity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">BAT</tok> <tok cat="NN">box</tok> <tok cat="NN">binding</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">BAT</tok> <tok cat="NN">box</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><cons cat="ADVP"><tok cat="RB">approximately</tok> <tok cat="RB">25-fold</tok></cons> <tok cat="JJR">less</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="FRAG"><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">octamer</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">BAT</tok> <tok cat="NN">box</tok> <tok cat="NN">binding</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBD">dissociated</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">BAT</tok> <tok cat="NN">box</tok></cons></cons> <cons cat="ADVP"><cons cat="ADVP" adv="TMP"><cons cat="ADVP"><tok cat="RB">10-fold</tok> <tok cat="RBR">more</tok></cons> <tok cat="RB">rapidly</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="FRAG"><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">octamer</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
768065310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Despite</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJR">lower</tok> <tok cat="NN">affinity</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i47" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">trimer</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">BAT</tok> <tok cat="NN">box</tok> <tok cat="NN">sequence</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><cons cat="ADVP"><tok cat="IN">as</tok> <tok cat="RB">efficiently</tok></cons> <cons cat="SBAR" ref="i100" null="ICH"></cons></cons><cons cat="VP"><tok cat="VBN">transactivated</tok> <cons cat="NP" ref="i47" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">an</tok> <tok cat="NN">Oct-2</tok> <tok cat="NN">expression</tok> <tok cat="NN">vector</tok></cons></cons> <cons cat="SBAR" id="i100"><tok cat="IN">as</tok> <cons cat="SINV"><tok cat="VBD">was</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">trimer</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">octamer</tok> <tok cat="NN">sequence</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">HeLa</tok> <tok cat="NNS">cells</tok></cons></cons></cons><cons cat="VP" null="QSTN"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
768065311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i48" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">BAT</tok> <tok cat="NN">box</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Oct-2</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP" ref="i48" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD20</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">pre-B</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">PB-697</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NNS">esters</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
768065312@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i49" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD20</tok> <tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">temporally</tok></cons> <tok cat="VBN">associated</tok> <cons cat="NP" ref="i49" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Oct-2</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i50" role="SBJ"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="NN">BAT</tok> <tok cat="JJ">box-deleted</tok></cons> <tok cat="NN">CD20-CAT</tok> <tok cat="NN">construct</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">contrast</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">wild</tok> <tok cat="NN">type</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">poorly</tok></cons> <tok cat="VBN">induced</tok> <cons cat="NP" ref="i50" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">phorbol</tok> <tok cat="NNS">esters</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
768065313@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Together</tok></cons> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">BAT</tok> <tok cat="NN">box</tok> <tok cat="NN">binding</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok></cons> <tok cat="JJ">specific</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" id="i101"><tok cat="NN">CD20</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">perhaps</tok></cons> <cons map="i101" cat="NP"><tok cat="NN">CD21</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
768224301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Functional</tok> <tok cat="NN">antagonism</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">vitamin</tok> <tok cat="NN">D3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CD14</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CD23</tok></cons></cons> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">U-937</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
768224302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="CD">1,25</tok> <tok cat="NN">alpha-Dihydroxicholecalciferol</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">VitD3</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">important</tok> <tok cat="NNS">regulators</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">proliferation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
768224303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">paper</tok></cons> <cons cat="VP"><tok cat="VBZ">describes</tok> <cons cat="SBAR"><cons cat="WHADVP" id="i24"><tok cat="WRB">how</tok></cons> <cons cat="S"><cons cat="NP" syn="COOD" id="i25" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">monocyte-macrophage</tok> <tok cat="NN">Ag</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CD14</tok></cons><tok cat="COMMA">,</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">low</tok> <tok cat="NN">affinity</tok> <tok cat="NN">Fc</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">IgE</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CD23</tok></cons><tok cat="COMMA">,</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">inversely</tok></cons> <tok cat="VBN">regulated</tok> <cons cat="NP" ref="i25" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><cons cat="NP"><tok cat="NN">VitD3-</tok></cons> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">RA-induced</tok></cons></cons> <tok cat="JJ">monocytic</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">U-937</tok> <tok cat="NNS">monoblasts</tok></cons></cons></cons></cons><cons cat="ADVP" ref="i24" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
768224304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PMA</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">CD14</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CD23</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
768224305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Exposure</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">VitD3</tok></cons></cons></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="FW">de</tok> <tok cat="FW">novo</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD14</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
768224306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cycloheximide</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">completely</tok></cons> <cons cat="VP"><tok cat="VBD">blocked</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">VitD3</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD14</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">ongoing</tok> <tok cat="NN">protein</tok> <tok cat="NN">synthesis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
768224307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">While</tok> <cons cat="S"><cons cat="NP" ref="i27" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inducing</tok> <cons cat="NP"><tok cat="NN">CD14</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i27" role="SBJ"><tok cat="NN">VitD3</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">concomitantly</tok></cons> <cons cat="VP"><tok cat="VBD">suppressed</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">basal</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><cons cat="NP"><tok cat="NN">PMA-</tok></cons><cons cat="ADJP" null="QSTN"></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">RA-inducible</tok></cons></cons> <tok cat="NN">CD23</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">dose-dependent</tok> <tok cat="NN">manner</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
768224308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">U-937</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">RA</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD23</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="ADVP"><tok cat="RB">completely</tok></cons> <cons cat="VP"><tok cat="VBD">lacked</tok> <cons cat="NP"><tok cat="JJ">detectable</tok> <tok cat="NN">CD14</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">surface</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">mRNA</tok></cons></cons> <tok cat="NN">expression</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
768224309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i28" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><cons cat="NP"><tok cat="NN">VitD3-</tok></cons> <cons cat="ADJP" null="QSTN"></cons></cons><cons cat="NP" ref="i29" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">PMA-induced</tok> <cons cat="NP" ref="i29" null="RNR"></cons></cons></cons><cons cat="NP" id="i29"><tok cat="NN">CD14</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i28" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">temporal</tok> <tok cat="NN">consequence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">RA-induced</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
768224310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBZ">exists</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">functional</tok> <tok cat="NN">antagonism</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">VitD3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">RA</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i30"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i30" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">important</tok> <tok cat="NNS">implications</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">certain</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">immune</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">inflammatory</tok></cons></cons> <tok cat="NNS">responses</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">inverse</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CD14</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CD23</tok></cons></cons> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
768331601@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Adenovirus</tok> <tok cat="NN">E1A</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">IFN-induced</tok> <tok cat="NN">resistance</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cytolysis</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">natural</tok> <tok cat="NN">killer</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
768331602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">target</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">cytopathic</tok> <tok cat="NNS">viruses</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">IFN</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cytolytic</tok> <tok cat="NN">resistance</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">NK</tok> <tok cat="JJ">cell-mediated</tok></cons> <tok cat="NN">cytolysis</tok></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP"><cons cat="NP"><tok cat="JJ">IFN-mediated</tok> <tok cat="NN">cytoprotection</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IFN-MCP</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="RRB">]</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
768331603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">thought</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">virally</tok> <tok cat="VBN">induced</tok></cons> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IFN-MCP</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">secondary</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">shutdown</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="JJ">macromolecular</tok> <tok cat="NN">synthesis</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i33"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i33" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">accompanies</tok> <cons cat="NP"><tok cat="JJ">cytopathic</tok> <tok cat="NN">virus</tok> <tok cat="NNS">infections</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
768331604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Group</tok> <tok cat="NN">C</tok><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">adenovirus</tok> <tok cat="NN">serotype</tok> <tok cat="CD">5</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Ad5</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">infection</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">IFN-MCP</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NN">protein</tok> <tok cat="NN">synthesis</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
768331605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBD">determined</tok> <cons cat="SBAR"><tok cat="IN">if</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">Ad5-induced</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IFN-MCP</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" syn="COOD" role="PRD"><cons cat="ADJP"><tok cat="JJ">independent</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">adenovirus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Ad</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">infection</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">secondary</tok> <cons cat="PP"><tok cat="RB">only</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Ad</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">region</tok> <tok cat="NN">1A</tok> <tok cat="NN">gene</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">E1A</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
768331606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">test</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">hypothesis</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i34" role="SBJ"><tok cat="JJ">4-h</tok> <tok cat="NN">NK</tok> <tok cat="NN">cytolysis</tok> <tok cat="NNS">assays</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">performed</tok> <cons cat="NP" ref="i34" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">IFN-gamma-treated</tok> <tok cat="JJ">human</tok> <tok cat="NNS">cells</tok></cons> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="VBN">infected</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">Ad5</tok> <tok cat="NN">E1A</tok> <tok cat="NN">deletion</tok> <tok cat="NN">mutant</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">dl343</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="VBN">transfected</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Ad5</tok> <tok cat="NN">E1A</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
768331607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i35" role="SBJ"><tok cat="NN">IFN-MCP</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i35" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">dl343</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">despite</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">large</tok> <tok cat="NNS">amounts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">early</tok> <cons cat="NP" ref="i36" null="RNR"></cons></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">E1B</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p55</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">late</tok> <cons cat="NP" ref="i36" null="RNR"></cons></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">hexon</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="NP" id="i36"><tok cat="NN">Ad</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
768331608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <tok cat="NN">contrast</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">E1A-negative</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">parental</tok></cons></cons> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i37" role="SBJ"><tok cat="NN">IFN-MCP</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i37" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">Ad5</tok> <tok cat="JJ">E1A-transfected</tok></cons> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">epithelial</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">fibroblastic</tok></cons></cons> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
768331609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Genetic</tok> <tok cat="NN">mapping</tok> <tok cat="NNS">studies</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">E1A</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="RB">only</tok> <tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NN">exon</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">E1A</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">sufficient</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><tok cat="NN">IFN-MCP</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
768331610@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i38" role="SBJ"><cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">sequence</tok> <tok cat="NN">homology</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">E1A</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NN">Ad</tok> <tok cat="NNS">groups</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="FRAG"><cons cat="FRAG"><cons cat="NP"><tok cat="NN">group</tok> <tok cat="NN">A</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Ad12</tok></cons></cons><tok cat="COLON">;</tok> <cons cat="FRAG"><cons cat="NP"><tok cat="NN">group</tok> <tok cat="NN">C</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Ad5</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">limited</tok> <cons cat="NP" ref="i38" null="NONE"></cons><cons cat="PP"><cons cat="ADVP"><tok cat="RB">almost</tok> <tok cat="RB">entirely</tok></cons> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok> <tok cat="VBN">conserved</tok> <tok cat="NNS">regions</tok></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NN">exon</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">E1A</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
768331611@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">Because</tok> <cons cat="S"><cons cat="NP" id="i39" role="SBJ"><tok cat="NN">IFN-MCP</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i39" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Ad12</tok> <tok cat="JJ">E1A-transfected</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i100" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">one</tok> <tok cat="CC">or</tok> <tok cat="JJR">more</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">E1A-conserved</tok> <tok cat="NNS">regions</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">necessary</tok> <cons cat="S"><cons cat="NP" ref="i100" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><tok cat="NN">IFN-MCP</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
768331612@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">summary</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">E1A</tok> <tok cat="NN">gene</tok> <tok cat="NNS">products</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="NN">IFN-MCP</tok></cons> <cons cat="ADVP"><tok cat="RB">independently</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">virus</tok> <tok cat="NN">infection</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
768331613@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">E1A</tok><tok cat="POS">'s</tok></cons> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IFN-MCP</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">net</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">promoting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">selective</tok> <cons cat="ADJP"><tok cat="NN">NK</tok> <tok cat="JJ">cell-mediated</tok></cons> <tok cat="NN">clearance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">Ad-infected</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">Ad-transformed</tok></cons></cons> <tok cat="JJ">human</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
768369201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">mutation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">cortisol</tok> <tok cat="NN">resistance</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
768369202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i24" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">precise</tok> <tok cat="JJ">molecular</tok> <tok cat="NNS">abnormalities</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i104"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i104" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">cause</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">cortisol</tok> <tok cat="NN">resistance</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">completely</tok></cons> <tok cat="VBN">described</tok><cons cat="NP" ref="i24" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
768369203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">subject</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">cortisol</tok> <tok cat="NN">resistance</tok></cons></cons></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">hGR</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="VBN">decreased</tok> <tok cat="NN">affinity</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">dexamethasone</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
768369204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">hypothesize</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">mutation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">hGR</tok> <tok cat="JJ">glucocorticoid-binding</tok> <tok cat="NN">domain</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cause</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cortisol</tok> <tok cat="NN">resistance</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
768369205@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i25" role="SBJ"><cons cat="NP"><tok cat="JJ">Total</tok> <tok cat="NN">RNA</tok></cons> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">index</tok> <tok cat="NN">subject</tok><tok cat="POS">'s</tok></cons> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">leukocytes</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i25" id="i26" null="NONE"></cons><cons cat="S"><cons cat="NP" ref="i26" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">produce</tok> <cons cat="NP"><tok cat="JJ">first</tok> <tok cat="NN">strand</tok> <tok cat="NN">hGR</tok> <tok cat="NNS">cDNAs</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i27" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">entire</tok> <tok cat="NN">hGR</tok> <tok cat="NN">cDNA</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">amplified</tok> <cons cat="NP" ref="i27" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">segments</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">sequenced</tok><cons cat="NP" ref="i27" null="NONE"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
768369206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">At</tok> <cons cat="NP"><tok cat="NN">nucleotide</tok> <tok cat="CD">2,317</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">homozygous</tok> <tok cat="NN">A</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">G</tok> <tok cat="NN">point</tok> <tok cat="NN">mutation</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i29"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i29" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">predicts</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">isoleucine</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ATT</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">valine</tok> <tok cat="LRB">(</tok><tok cat="NN">GTT</tok><tok cat="RRB">)</tok> <tok cat="NN">substitution</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="NN">amino</tok> <tok cat="NN">acid</tok> <tok cat="CD">729</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
768369207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i30"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" syn="COOD" id="i31" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">wild-type</tok> <tok cat="NN">hGR</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">hGR-Ile</tok> <tok cat="CD">729</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i31" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">COS-1</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">assayed</tok> <cons cat="NP" ref="i31" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">[3H]-Dexamethasone</tok> <tok cat="NN">binding</tok></cons></cons></cons></cons><cons cat="ADVP" ref="i30" null="T"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">dissociation</tok> <tok cat="NNS">constants</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="NP" role="PRD"><cons cat="NP"><cons cat="QP" syn="COOD"><cons cat="QP"><tok cat="CD">0.799</tok> <tok cat="CC">+/-</tok> <tok cat="CD">0.068</tok></cons> <tok cat="CC">and</tok> <cons cat="QP"><tok cat="CD">1.54</tok> <tok cat="CC">+/-</tok> <tok cat="CD">0.06</tok></cons></cons> <tok cat="NN">nM</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">mean</tok></cons> <tok cat="CC">+/-</tok> <cons cat="NP"><tok cat="NN">SEM</tok></cons></cons><tok cat="RRB">)</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons> <cons cat="NP" role="PRD"><cons cat="QP"><tok cat="JJR">&lt;</tok> <tok cat="CD">0.01</tok></cons></cons></cons><tok cat="RRB">)</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
768369208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i34"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" syn="COOD" id="i35" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">wild-type</tok> <tok cat="NN">hGR</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">hGR-Ile</tok> <tok cat="CD">729</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i35" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CV-1</tok> <tok cat="NNS">cells</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i36"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i36" id="i37" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">cotransfected</tok> <cons cat="NP" ref="i37" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mouse</tok> <tok cat="JJ">mammary</tok> <tok cat="NN">tumor</tok> <tok cat="NN">virus</tok> <tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok></cons> <cons cat="VP"><tok cat="VBN">fused</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">chloramphenicol</tok> <tok cat="NN">acetyl</tok> <tok cat="NN">transferase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CAT</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i34" null="T"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">hGR-Ile</tok> <tok cat="CD">729</tok></cons> <cons cat="VP"><tok cat="VBD">conferred</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">fourfold</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">apparent</tok> <tok cat="NN">potency</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">dexamethasone</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CAT</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@52@@oe@16-12-2010
768369209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">isoleucine</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">valine</tok> <tok cat="NN">substitution</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="NN">amino</tok> <tok cat="NN">acid</tok> <tok cat="CD">729</tok></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">impairs</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">hGR</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">likely</tok> <tok cat="NN">cause</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">cortisol</tok> <tok cat="NN">resistance</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">subject</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
768450301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">Regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-1</tok> <tok cat="NN">beta</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="NN">beta</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">gene</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">mycobacterial</tok> <tok cat="NNS">components</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">lipopolysaccharide</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="CD">two</tok> <tok cat="JJ">nuclear</tok> <tok cat="JJ">factor-IL6</tok> <tok cat="NNS">motifs</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
768450302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">cytokines</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-1</tok> <tok cat="NN">beta</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="NN">beta</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">released</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">mononuclear</tok> <tok cat="NNS">phagocytes</tok></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NNS">mycobacteria</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">considered</tok> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">mediate</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">pathophysiologic</tok> <tok cat="NNS">events</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">granuloma</tok> <tok cat="NN">formation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">systemic</tok> <tok cat="NNS">symptoms</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
768450303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">Mycobacterium</tok> <tok cat="NN">tuberculosis</tok> <tok cat="NN">cell</tok> <tok cat="NN">wall</tok> <tok cat="NN">component</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lipoarabinomannan</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LAM</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">very</tok> <tok cat="JJ">potent</tok></cons> <tok cat="NN">inducer</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="NN">beta</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">effect</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
768450304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">localized</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><cons cat="NP"><tok cat="NN">LAM-</tok></cons><cons cat="ADJP" null="QSTN"></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><cons cat="NP"><cons cat="NP"><tok cat="NN">lipopolysaccharide</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LPS</tok></cons><tok cat="RRB">)</tok></cons><tok cat="COLON">-</tok></cons><cons cat="ADJP" null="QSTN"></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">TNF-alpha-inducible</tok></cons></cons> <tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><cons cat="ADJP"><tok cat="CD">-131/+15</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">positions</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-131</tok></cons> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="CD">+15</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">DNA</tok> <tok cat="NN">fragment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-1</tok> <tok cat="NN">beta</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">deletion</tok> <tok cat="NN">analysis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">chloramphenicol</tok> <tok cat="NN">acetyltransferase</tok> <tok cat="NN">assay</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
768450305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Within</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">DNA</tok> <tok cat="NN">fragment</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="NP" role="PRD"><cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">novel</tok> <tok cat="JJ">9-bp</tok> <tok cat="NNS">motifs</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-90/-82</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-40/-32</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NN">homology</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-IL6</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-IL6</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">binding</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
768450306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Site-directed</tok> <tok cat="NN">mutagenesis</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="NN">NF-IL-6</tok> <tok cat="NNS">motifs</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">independently</tok></cons> <tok cat="VBN">activated</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">LAM</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">LPS</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBD">acted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">orientation-independent</tok> <tok cat="NN">manner</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
768450307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">DNA</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">protein</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">LAM-</tok><cons cat="ADJP" null="NONE"></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="NN">LPS-</tok><cons cat="ADJP" null="NONE"></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">TNF-alpha- stimulated</tok></cons></cons> <tok cat="NN">THP-1</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-IL6</tok> <tok cat="NNS">motifs</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
768450308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="NN">NF-IL6</tok> <tok cat="NNS">sites</tok></cons> <cons cat="VP"><tok cat="VBP">mediate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="NN">beta</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">stimuli</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">LAM</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">LPS</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
768859601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Cytokine</tok> <tok cat="NN">modulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">expression</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@6@@oe@16-12-2010
768859602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i23" role="SBJ"><cons cat="NP"><tok cat="NNS">Cytokines</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">peptide</tok> <tok cat="NNS">hormones</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i24"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i24" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">control</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">homeostasis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immune</tok> <tok cat="NN">system</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <tok cat="RB">also</tok> <cons cat="VP"><tok cat="VBP">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">fundamental</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">inflammatory</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">immune</tok> <tok cat="JJ">mediated</tok></cons></cons> <tok cat="NNS">reactions</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i23" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">multiple</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathogenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">acquired</tok> <tok cat="JJ">immune</tok> <tok cat="NN">deficiency</tok> <tok cat="NN">syndrome</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AIDS</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
768859603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i25" role="SBJ"><cons cat="NP"><tok cat="NN">Infection</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HIV</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i25" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NNS">cytokines</tok></cons></cons></cons> <cons cat="ADVP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
768859604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Conversely</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="JJ">several</tok> <tok cat="NNS">cytokines</tok></cons> <cons cat="VP"><tok cat="VBP">modulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">infected</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADJP"><tok cat="NN">T</tok> <tok cat="JJ">lymphocytic</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">mononuclear</tok> <tok cat="JJ">phagocytic</tok></cons></cons> <tok cat="NN">lineage</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
768859605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="VBN">Activated</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="RB">particularly</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i26"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i26" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="PP" role="PRD"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">state</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">chronic</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">HIV</tok> <tok cat="JJ">infected</tok></cons> <tok cat="NNS">individuals</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">release</tok> <cons cat="NP"><tok cat="JJ">HIV-inductive</tok> <tok cat="NNS">cytokines</tok></cons></cons> <tok cat="CC">and</tok> <tok cat="RB">thus</tok> <cons cat="VP"><tok cat="VBP">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">potentially</tok> <tok cat="JJ">important</tok></cons> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathogenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">infection</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
769265201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">FK506</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">ciclosporin</tok></cons></cons><tok cat="COLON">:</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">molecular</tok> <tok cat="NNS">probes</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">studying</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
769265202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i33" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">immunosuppressants</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ciclosporin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">FK506</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">block</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">Ca(2+)-dependent</tok> <tok cat="NN">signal-transduction</tok> <tok cat="NN">pathway</tok></cons> <cons cat="VP"><tok cat="VBG">emanating</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" ref="i33" role="SBJ" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">thereby</tok></cons> <cons cat="VP"><tok cat="VBG">inhibiting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">helper</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
769265203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" ref="i34" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">drugs</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="NNS">probes</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i34" role="SBJ"><cons cat="NP"><tok cat="NNS">chemists</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">biologists</tok></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">uncovered</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="JJ">intracellular</tok> <tok cat="NN">signalling</tok> <tok cat="NNS">molecules</tok></cons> <cons cat="VP"><tok cat="VBG">bridging</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">generation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">second-messenger</tok> <tok cat="NN">Ca2+</tok> <tok cat="NNS">ions</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHPP" id="i35"><tok cat="IN">among</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="SINV"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="PP" ref="i35" role="PRD" null="T"></cons></cons><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">calmodulin</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">calcineurin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-AT</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@16-12-2010
769265204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="ADVP"><tok cat="RB">Hence</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">Ca2+</tok></cons> <cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">calmodulin</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">calmodulin</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">calcineurin</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="VBN">activated</tok> <tok cat="NN">calcineurin</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">turn</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">dephosphorylate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cytoplasmic</tok> <tok cat="NN">subunit</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytoplasm</tok></cons></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i100" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">form</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">competent</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activator</tok></cons><cons cat="ADVP" ref="i100" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@48@@oe@16-12-2010
769265205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">As</tok> <cons cat="S"><cons cat="NP" id="i37" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">described</tok> <cons cat="NP" ref="i37" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NNP">Jun</tok> <tok cat="NNP">Liu</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">drugs</tok></cons> <cons cat="VP"><tok cat="VBP">manifest</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">unprecedented</tok> <tok cat="NN">fashion</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
769265206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">They</tok></cons> <cons cat="VP"><tok cat="VBP">do</tok> <tok cat="RB">not</tok> <cons cat="ADVP"><tok cat="RB">directly</tok></cons> <cons cat="VP"><tok cat="VB">intercept</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">signalling</tok> <tok cat="NNS">molecules</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
769265207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Instead</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i38" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">form</tok> <cons cat="NP"><tok cat="JJ">tight</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">different</tok> <tok cat="NNS">classes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">abundant</tok> <tok cat="JJ">cytosolic</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="VP"><tok cat="VBN">called</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NNS">immunophilins</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">upon</tok> <cons cat="S"><cons cat="NP" ref="i38" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">entering</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">consequently</tok></cons> <tok cat="VBP">inhibit</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">peptidyl</tok> <tok cat="JJ">prolyl</tok> <tok cat="JJ">cis-trans</tok> <tok cat="NN">isomerase</tok> <tok cat="NNS">activities</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
769265208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="CD">two</tok> <cons cat="ADJP"><tok cat="RB">structurally</tok> <tok cat="JJ">distinct</tok></cons> <tok cat="JJ">immunophilin-drug</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">bind</tok> <cons cat="PP"><tok cat="TO">to</tok><cons cat="NP" ref="i39" null="RNR"></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">inhibit</tok><cons cat="NP" ref="i39" null="RNR"></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="NP" id="i39"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">phosphatase</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">calcineurin</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
769290601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">latency</tok> <tok cat="NN">reactivation</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">dehydroepiandrosterone</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">DHEA</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">analog</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DHEA</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
769290602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">initial</tok> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HIV-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJS">most</tok> <tok cat="NNS">individuals</tok></cons></cons></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">usually</tok></cons> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">establishment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">latent</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">chronic</tok></cons></cons> <tok cat="NN">infection</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">before</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">eventual</tok> <tok cat="NN">progression</tok></cons> <cons cat="PP"><tok cat="IN">toward</tok> <cons cat="NP"><tok cat="VBN">acquired</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">syndrome</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
769290603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HIV-1</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VB">establish</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">latent</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">persistent</tok></cons></cons> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">some</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">show</tok> <cons cat="NP"><tok cat="JJ">minimal</tok> <tok cat="JJ">constitutive</tok> <tok cat="NN">virus</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
769290604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="VBN">enhanced</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">replication</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">antigens</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNS">mitogens</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons><tok cat="RRB">]</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="CD">1</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">interleukin-2</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
769290605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Various</tok> <tok cat="NN">gene</tok> <tok cat="NNS">products</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">viruses</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HTLV-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">HSV</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">EBV</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CMV</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">HBV</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HHV-6</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VB">enhance</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <cons cat="ADJP"><tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok> <tok cat="LRB">(</tok><tok cat="NN">LTR</tok><tok cat="RRB">)</tok><tok cat="JJ">-driven</tok></cons> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok> <tok cat="NN">activity</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
769290606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">On</tok> <tok cat="DT">the</tok> <tok cat="NN">basis</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">observations</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">proposed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">reactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">latent</tok> <tok cat="NN">HIV-1</tok></cons> <cons cat="VP"><tok cat="VBN">harbored</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">chronically</tok> <tok cat="JJ">infected</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathogenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AIDS</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
769290607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">So</tok> <tok cat="RB">far</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">no</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">drugs</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">therapy</tok></cons></cons></cons> <cons cat="ADJP"><tok cat="JJ">available</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">protection</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">latency</tok> <tok cat="NN">reactivation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
769290608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">ACH-2</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CEM</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">chronically</tok> <tok cat="VBN">infected</tok> <cons cat="PP"><tok cat="IN">with</tok> <tok cat="NN">HIV-1</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">low</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">constitutive</tok> <tok cat="NN">virus</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
769290609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="NN">ACH-2</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">converted</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">productive</tok> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">12-O-tetradecanoylphorbol-13-acetate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TPA</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">mitogen</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cytokines</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">HSV</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
769290610@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">ACH-2</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">good</tok> <tok cat="NN">candidate</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">studying</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">drugs</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
769290611@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Previously</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">reported</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">DHEA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">synthetic</tok> <tok cat="NNS">analogs</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DHEA</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">modest</tok> <tok cat="NNS">inhibitors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">IIIB</tok> <tok cat="NN">replication</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">phytohemagglutinin-stimulated</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NNS">cultures</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
769290612@GENIA Treebank@formal@@1@S@<cons cat="S"><tok cat="LRB">(</tok><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">ABSTRACT</tok></cons> <cons cat="VP"><tok cat="VBN">TRUNCATED</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">AT</tok> <cons cat="NP"><tok cat="CD">250</tok> <tok cat="NNS">WORDS</tok></cons></cons></cons><tok cat="RRB">)</tok></cons>@@@@1@7@@oe@16-12-2010
770623501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">Nitric</tok> <tok cat="JJ">oxide-stimulated</tok></cons> <tok cat="NN">guanine</tok> <tok cat="NN">nucleotide</tok> <tok cat="NN">exchange</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">p21ras</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@8@@oe@16-12-2010
770623502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">protooncogene</tok> <tok cat="NN">p21ras</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">monomeric</tok> <tok cat="NN">G</tok> <tok cat="NN">protein</tok> <tok cat="NN">family</tok> <tok cat="NN">member</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">critical</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">converting</tok> <cons cat="NP"><tok cat="JJ">extracellular</tok> <tok cat="NNS">signals</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="JJ">biochemical</tok> <tok cat="NNS">events</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@16-12-2010
770623503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">nitric</tok> <tok cat="NN">oxide</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NO</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="NN">p21ras</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">evidenced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">GTP-bound</tok> <tok cat="NN">p21ras</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
770623504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">In</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="JJ">pure</tok> <tok cat="JJ">recombinant</tok> <tok cat="NN">p21ras</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" syn="COOD" role="PRD"><cons cat="ADJP"><tok cat="JJ">direct</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">reversible</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
770623505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Circular</tok> <tok cat="NN">dichroism</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBZ">reveals</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NO</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">profound</tok> <tok cat="JJ">conformational</tok> <tok cat="NN">change</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">p21ras</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">association</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">GDP/GTP</tok> <tok cat="NN">exchange</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
770623506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">activation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">due</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">S-nitrosylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">critical</tok> <tok cat="NN">cysteine</tok> <tok cat="NN">residue</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><tok cat="NN">guanine</tok> <tok cat="NN">nucleotide</tok> <tok cat="NN">exchange</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
770623507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">p21ras</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">NO-induced</tok> <tok cat="JJ">downstream</tok> <tok cat="NN">signaling</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">endogenous</tok> <tok cat="NN">NO</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="NN">p21ras</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NN">cell</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
770623508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">identify</tok> <cons cat="NP"><tok cat="NN">p21ras</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">target</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NN">cell</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
770623509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">identify</tok> <cons cat="NP"><tok cat="NN">p21ras</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">target</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NO</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NO</tok></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="NN">p21ras</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">action</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">mimics</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">guanine</tok> <tok cat="NN">nucleotide</tok> <tok cat="NN">exchange</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
770627301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Transcriptional</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">vacuolar</tok> <tok cat="NN">H(+)-ATPase</tok> <tok cat="NN">B2</tok> <tok cat="NN">subunit</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBG">differentiating</tok> <tok cat="NN">THP-1</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@16-12-2010
770627302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="JJ">Monocyte-macrophage</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">model</tok> <tok cat="NN">system</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">studying</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">vacuolar</tok> <tok cat="NN">H(+)-ATPase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">V-ATPase</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@16-12-2010
770627303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">mRNA</tok></cons> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">various</tok> <cons cat="NP"><tok cat="NN">V-ATPase</tok></cons> <tok cat="NNS">subunits</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="JJ">native</tok> <tok cat="NNS">monocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="NP"><tok cat="NN">THP-1</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">transcriptional</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">post-transcriptional</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">mechanisms</tok></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">account</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">increases</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">cell</tok> <cons cat="NP"><tok cat="NN">V-ATPase</tok></cons> <tok cat="NN">content</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
770627304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">From</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="JJ">runoff</tok> <tok cat="NNS">experiments</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="CD">one</tok> <tok cat="NN">subunit</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">particular</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">B2</tok> <tok cat="NN">isoform</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">Mr</tok></cons> <cons cat="ADJP"><tok cat="JJ">=</tok> <cons cat="NP"><tok cat="CD">56,000</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">amplified</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">primarily</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NNS">means</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
770627305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">begun</tok> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">examine</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">structure</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">B2</tok> <tok cat="NN">subunit</tok> <tok cat="NN">promoter</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
770627306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Isolation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">sequencing</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">first</tok> <tok cat="NN">exon</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">5'-flanking</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">reveal</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">TATA-less</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">high</tok> <tok cat="NN">G</tok> <tok cat="CC">+</tok> <tok cat="NN">C</tok> <tok cat="NN">content</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
770627307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Primer</tok> <tok cat="NN">extension</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">ribonuclease</tok></cons> <tok cat="NN">protection</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">analyses</tok></cons></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">single</tok> <cons cat="NP"><tok cat="JJ">major</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">start</tok> <tok cat="NN">site</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
770627308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">transfected</tok> <cons cat="NP"><tok cat="NN">promoter-luciferase</tok> <tok cat="NN">reporter</tok> <tok cat="NNS">plasmids</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="NN">THP-1</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">define</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">sequences</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">mediate</tok> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">control</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">monocyte</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
770627309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">sequences</tok></cons> <cons cat="ADVP"><tok cat="RB">downstream</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">start</tok> <tok cat="NN">site</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">sufficient</tok></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">confer</tok> <cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">THP-1</tok></cons> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
770627310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">DNase</tok> <tok cat="CD">I</tok></cons> <cons cat="NP"><tok cat="NN">footprinting</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">sequence</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NN">analysis</tok></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">existence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">multiple</tok></cons> <cons cat="NP"><tok cat="NN">AP2</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Sp1</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">5'-untranslated</tok></cons> <cons cat="NP" ref="i24" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">proximal</tok> <tok cat="VBG">coding</tok></cons> <cons cat="NP" ref="i24" null="RNR"></cons></cons><cons cat="NP" id="i24"><tok cat="NNS">regions</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
770671001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Functional</tok> <tok cat="NN">characterization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">novel</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="JJ">transcriptional</tok> <tok cat="NNS">inhibitors</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@8@@oe@16-12-2010
770671002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">IL-2-mediated</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok></cons> <tok cat="NN">proliferation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">critical</tok></cons> <cons cat="ADJP"><tok cat="JJ">early</tok></cons></cons> <tok cat="NN">event</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inflammatory</tok> <tok cat="NN">process</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@16-12-2010
770671003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Formation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NFAT-1</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">complex</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="NN">transcription</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
770671004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" id="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">stably</tok></cons> <cons cat="VP"><tok cat="VBN">transfected</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">trimer</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NFAT-1</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">element</tok></cons></cons> <cons cat="VP"><tok cat="VBN">linked</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">lac-Z</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">screened</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">inhibitors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">NFAT-1-mediated</tok> <cons cat="NP"><tok cat="NN">beta-galactosidase</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
770671005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">WIN</tok> <tok cat="CD">61058</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">WIN</tok> <tok cat="CD">53071</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">microM</tok> <tok cat="NNS">inhibitors</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
770671006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">compounds</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">beta-galactosidase</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="NNS">levels</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@16-12-2010
770671007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="JJ">Similar</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">NFAT-1-mediated</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">second</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i11"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i11" id="i12" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">stably</tok></cons> <cons cat="VP"><tok cat="VBN">transfected</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NFAT-1</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">elements</tok></cons> <cons cat="VP"><tok cat="VBN">linked</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">reporter</tok> <tok cat="NN">gene</tok> <tok cat="IN">for</tok> <tok cat="NN">sCD8</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
770671008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">At</tok> <cons cat="NP"><tok cat="CD">10</tok> <tok cat="NN">microM</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">both</tok> <tok cat="NNS">compounds</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">mRNA</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">levels</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">NFAT-1-linked</tok> <tok cat="NN">lac-Z</tok> <tok cat="NNS">transfectants</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
770671009@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">Both</tok> <tok cat="NNS">compounds</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">mixed</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NN">reaction</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i13" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">reversed</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">exogenous</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
770671010@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">WIN</tok> <tok cat="CD">53071</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="NN">production</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="JJ">calcium-dependent</tok></cons> <cons cat="NP"><tok cat="NN">PMA</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">ionomycin</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">pathway</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
770671011@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Conversely</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">calcium-independent</tok> <tok cat="JJ">anti-CD28</tok> <tok cat="NN">Ab</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">PMA-induced</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons> <tok cat="NN">production</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">resistant</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
770671012@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i14" role="SBJ"><tok cat="DT">Both</tok> <tok cat="NNS">compounds</tok></cons> <cons cat="VP"><tok cat="VBD">altered</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NFAT-1</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">complex</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i14" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">causing</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="VBN">retarded</tok> <tok cat="NN">mobility</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NNS">gels</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
770671013@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="JJ">functional</tok> <tok cat="NNS">criteria</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">believe</tok> <cons cat="COMP" null="ZERO"></cons><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADVP"><tok cat="RB">structurally</tok></cons> <cons cat="ADJP"><tok cat="JJ">distinct</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">novel</tok></cons></cons> <tok cat="NNS">inhibitors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">NFAT-1-mediated</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
770672701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">cDNA</tok></cons> <tok cat="NN">cloning</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">NGFI-B/nur77-related</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">apoptotic</tok> <tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
770672702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="JJ">lymphoid</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">PEER</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">dies</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PMA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">calcium</tok> <tok cat="NN">ionophore</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@16-12-2010
770672703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">new</tok> <cons cat="NP"><tok cat="NN">gene</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">TINUR</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">cloned</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">apoptotic</tok> <tok cat="NN">PEER</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
770672704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TINUR</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">1</tok> <tok cat="NN">h</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cross-linking</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">Ag</tok> <tok cat="NN">receptor</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
770672705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">TINUR</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">belongs</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NGFI-B/nur77</tok> <tok cat="NN">family</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">steroid</tok> <tok cat="NN">receptor</tok> <tok cat="NN">superfamily</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">orphan</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
770672706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">TINUR</tok></cons> <cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">sequence</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">NGFI-B/nur77</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
770672707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">propose</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NGFI-B/nur77</tok> <tok cat="NN">family</tok></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">classified</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">subtypes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
770698301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">Effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">intranasal</tok> <tok cat="NNS">glucocorticoids</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">endogenous</tok></cons> <tok cat="NN">glucocorticoid</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">peripheral</tok></cons> <cons cat="NP" ref="i30" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">central</tok></cons> <cons cat="NP" ref="i30" null="RNR"></cons></cons><cons cat="NP" id="i30"><tok cat="NN">function</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@12@@oe@16-12-2010
770698302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">Glucocorticoids</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="PP" role="PRD"><tok cat="IN">among</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RBS">most</tok> <tok cat="JJ">potent</tok> <tok cat="JJ">antiinflammatory</tok></cons> <tok cat="NNS">agents</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" id="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">rhinitis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@16-12-2010
770698303@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRPP">Their</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">action</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" syn="COOD" role="PRD"><cons cat="ADJP"><tok cat="JJ">multiple</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">complex</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">reports</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">describe</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">significant</tok></cons> <cons cat="ADJP"><tok cat="JJ">systemic</tok></cons></cons> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">locally</tok> <tok cat="VBN">administered</tok></cons> <tok cat="NNS">glucocorticoids</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
770698304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">In</tok> <tok cat="NN">order</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">evaluate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="JJ">short-term</tok> <tok cat="JJ">systemic</tok></cons> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">intranasally</tok> <tok cat="VBN">administered</tok></cons> <tok cat="NNS">glucocorticoids</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="CD">14</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">normal</tok></cons> <cons cat="ADJP"><tok cat="JJ">healthy</tok></cons></cons> <tok cat="NNS">subjects</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">doses</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">budesonide</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">BUD</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">fluticasone</tok> <tok cat="NN">propionate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">FP</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="CD">2</tok> <tok cat="NNS">weeks</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
770698305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="UCP" syn="COOD"><cons cat="PP" adv="TMP"><tok cat="IN">Before</tok> <cons cat="NP"><tok cat="NN">treatment</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">regular</tok> <tok cat="NNS">intervals</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">treatment</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" func="ADV"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">1</tok> <tok cat="NN">week</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">finally</tok></cons> <cons cat="NP"><tok cat="CD">6</tok> <tok cat="NNS">weeks</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">termination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">treatment</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GR</tok></cons><tok cat="RRB">)</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">methallothionein</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MTIIa</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">levels</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">solution</tok> <tok cat="NN">hybridization</tok> <tok cat="NN">assay</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@48@@oe@16-12-2010
770698306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i10" role="SBJ"><cons cat="NP"><tok cat="NN">Serum</tok> <tok cat="NN">cortisol</tok><cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">osteocalcin</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">urinary</tok> <tok cat="NN">cortisol</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">levels</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">determined</tok><cons cat="NP" ref="i10" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
770698307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="DT">An</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">insulin</tok> <tok cat="NN">tolerance</tok> <tok cat="NN">test</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ITT</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">performed</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP" syn="COOD" adv="TMP"><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">end</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">second</tok> <tok cat="NN">week</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">treatment</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">end</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">6-week</tok> <tok cat="NN">washout</tok> <tok cat="NN">period</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">no</tok> <cons cat="ADJP"><tok cat="RB">statistically</tok> <tok cat="JJ">significant</tok></cons> <tok cat="NN">change</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">cortisol</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
770698308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NNS">lymphocytes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="NN">GR</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP"><tok cat="VBN">down-regulated</tok><cons cat="NP" ref="i12" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
770698309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">MTIIa</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@6@@oe@16-12-2010
770698310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Serum</tok> <cons cat="NP"><tok cat="NN">osteocalcin</tok></cons></cons> <cons cat="VP"><tok cat="VBD">decreased</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">BUD</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">FP</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
770698311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Serum</tok> <tok cat="NN">cortisol</tok></cons> <cons cat="VP"><tok cat="VBD">decreased</tok> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">1</tok> <tok cat="NN">week</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">treatment</tok></cons></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" id="i15" role="SBJ"><tok cat="JJ">urinary</tok> <tok cat="NN">cortisol</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">affected</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">until</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">second</tok> <tok cat="NN">week</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">treatment</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
770698312@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">conclusion</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">intranasal</tok> <tok cat="NNS">glucocorticoids</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="ADJP"><tok cat="RB">clinically</tok> <tok cat="VBN">recommended</tok></cons> <tok cat="NNS">doses</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">have</tok> <cons cat="CONJP"><tok cat="RB">not</tok> <tok cat="RB">only</tok></cons> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">significant</tok> <tok cat="JJ">systemic</tok></cons> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">adrenal</tok> <tok cat="NN">function</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
770698313@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">effects</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" syn="COOD" role="PRD"><cons cat="ADJP"><tok cat="JJ">receptor-dependent</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">reversible</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="VBG">according</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">serum</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">urinary</tok> <tok cat="NN">cortisol</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NNS">levels</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">ITT</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBP">leave</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">hypothalamic-pituitary-adrenal</tok> <tok cat="NN">function</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">intact</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
770698314@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i16" role="SBJ"><tok cat="DT">these</tok> <cons cat="ADJP"><tok cat="JJ">short-term</tok> <tok cat="JJ">systemic</tok></cons> <tok cat="NNS">effects</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i16" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">any</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">noticeable</tok> <tok cat="NNS">side-effects</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">usually</tok></cons> <tok cat="VBN">observed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">long-term</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
770751401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="NN">core</tok> <tok cat="NN">binding</tok> <cons cat="NP"><tok cat="NN">factor-alpha</tok> <tok cat="LRB">(</tok><tok cat="NN">AML1</tok><tok cat="RRB">)</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><tok cat="NN">beta</tok></cons> <tok cat="NNS">subunits</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">enhancer</tok> <tok cat="NNS">cores</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
770751402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" id="i5"><cons cat="NP"><tok cat="NN">Core</tok> <tok cat="NN">binding</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CBF</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">polyomavirus</tok> <tok cat="JJ">enhancer-binding</tok> <tok cat="NN">protein</tok> <tok cat="CD">2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">SL3</tok> <tok cat="NN">enhancer</tok> <tok cat="NN">factor</tok> <tok cat="CD">1</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">mammalian</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">element</tok></cons> <cons cat="VP"><tok cat="VBN">termed</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">core</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NNS">enhancers</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">murine</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">family</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">retroviruses</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@45@@oe@16-12-2010
770751403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">core</tok> <tok cat="NNS">elements</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">SL3</tok> <tok cat="NN">virus</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">important</tok> <tok cat="JJ">genetic</tok> <tok cat="NNS">determinants</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">virus</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NNS">lymphomas</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
770751404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">CBF</tok></cons> <cons cat="VP"><tok cat="VBZ">consists</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">subunits</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">subunit</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CBF</tok> <tok cat="NN">alpha</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">second</tok> <tok cat="NN">subunit</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CBF</tok> <tok cat="NN">beta</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CBF</tok> <tok cat="NN">alpha</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
770751405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="CD">One</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i29"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i29" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">encodes</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">CBF</tok> <tok cat="NN">alpha</tok> <tok cat="NN">subunit</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="NN">AML1</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">called</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NN">Cbf</tok> <tok cat="NN">alpha</tok> <tok cat="CD">2</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
770751406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">locus</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">rearranged</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">chromosomal</tok> <tok cat="NNS">translocations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">myeloproliferative</tok> <tok cat="NNS">disorders</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">leukemias</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
770751407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">An</tok> <cons cat="ADJP"><tok cat="RB">exogenously</tok> <tok cat="VBN">expressed</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">Cbf</tok> <tok cat="NN">alpha</tok> <tok cat="JJ">2-encoded</tok> <tok cat="NN">subunit</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CBF</tok> <tok cat="NN">alpha</tok> <tok cat="CD">2-451</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">stimulated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">SL3</tok> <tok cat="NN">enhancer</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">P19</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HeLa</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
770751408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="NN">Activity</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">core</tok> <tok cat="NNS">elements</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@16-12-2010
770751409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="CD">Three</tok> <tok cat="JJ">different</tok> <tok cat="NNS">isoforms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CBF</tok> <tok cat="NN">beta</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">tested</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">SL3</tok> <tok cat="NN">enhancer</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
770751410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJS">longest</tok> <tok cat="NN">form</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CBF</tok> <tok cat="NN">beta-187</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">CBF</tok> <tok cat="NN">alpha</tok> <tok cat="CD">2-451</tok></cons></cons></cons> <cons cat="NP" func="ADV"><tok cat="JJ">twofold</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">HeLa</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">P19</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
770751411@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Transcriptional</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">CBF</tok> <tok cat="NN">beta</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">required</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CBF</tok> <tok cat="NN">alpha</tok> <tok cat="NN">subunit</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i16" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CBF</tok> <tok cat="NN">beta</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i15"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i15" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">lacked</tok> <cons cat="NP"><tok cat="NN">binding</tok> <tok cat="NN">ability</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CBF</tok> <tok cat="NN">beta-148</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">failed</tok> <cons cat="S"><cons cat="NP" ref="i16" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">increase</tok> <cons cat="NP"><tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
770751412@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBD">indicated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><cons cat="ADVP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">certain</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">maximum</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CBF</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">required</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">subunits</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
770751413@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">They</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">provided</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">support</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">hypothesis</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">CBF</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i17"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i17" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">responsible</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">recognition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">SL3</tok> <tok cat="NNS">cores</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
770751414@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">CBF</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">distinguish</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">1-bp</tok> <tok cat="NN">difference</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">enhancer</tok> <tok cat="NN">core</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SL3</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">core</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nonleukemogenic</tok> <tok cat="NN">virus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Akv</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
770751415@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">difference</tok></cons> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBZ">affects</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">leukemogenicity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SL3</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
770751416@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i18" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">CBF</tok> <tok cat="NN">alpha</tok></cons> <cons cat="NP" ref="i25" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CBF</tok> <tok cat="NN">beta</tok></cons> <cons cat="NP" ref="i25" null="RNR"></cons></cons><cons cat="NP" id="i25"><tok cat="NNS">subunits</tok></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i26"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">tested</tok> <cons cat="NP" ref="i26" null="T"></cons></cons></cons></cons></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i18" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">distinguish</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">1-bp</tok> <tok cat="NN">difference</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">assays</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
770751417@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i27" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">complete</tok> <tok cat="NN">understanding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="SBAR"><cons cat="WHADVP" id="i30"><tok cat="WRB">how</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">recognize</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">SL3</tok> <tok cat="NN">core</tok></cons></cons> <cons cat="ADVP" ref="i30" null="T"></cons></cons></cons></cons></cons></cons><cons cat="VP"><tok cat="VBZ">remains</tok> <cons cat="S"><cons cat="NP" ref="i27" id="i28" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">elucidated</tok><cons cat="NP" ref="i28" null="NONE"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
771386801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Platelet-activating</tok> <tok cat="NN">factor</tok></cons> <cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">heparin-binding</tok> <tok cat="JJ">epidermal</tok> <tok cat="NN">growth</tok> <tok cat="JJ">factor-like</tok> <tok cat="NN">growth</tok> <tok cat="NN">factor</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
771386802@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Correlation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="VBN">increased</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
771386803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons> <cons cat="VP"><tok cat="VBD">responded</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">platelet-activating</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PAF</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">up-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcript</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">heparin-binding</tok> <tok cat="JJ">epidermal</tok> <tok cat="NN">growth</tok> <tok cat="JJ">factor-like</tok> <tok cat="NN">growth</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HB-EGF</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">potent</tok> <tok cat="NN">mitogen</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">vascular</tok> <tok cat="JJ">smooth</tok> <tok cat="NN">muscle</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@38@@oe@16-12-2010
771386804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">This</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PAF</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nanomolar</tok> <tok cat="NNS">concentrations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ligand</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">starting</tok> <cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">30</tok> <tok cat="NN">min</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
771386805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">PAF-induced</tok> <tok cat="NN">up-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HB-EGF</tok> <tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">accompanied</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
771386806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">These</tok> <tok cat="NNS">functions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PAF</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">appeared</tok> <cons cat="S"><cons cat="NP" ref="i8" id="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <tok cat="NN">PAF</tok> <tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">two</tok> <cons cat="NP"><tok cat="NN">PAF</tok></cons> <tok cat="NN">receptor</tok> <tok cat="NNS">antagonists</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">WEB</tok> <tok cat="CD">2086</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">L-659</tok><tok cat="COMMA">,</tok><tok cat="CD">989</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">blocked</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">up-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HB-EGF</tok> <tok cat="NN">mRNA</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">PAF</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
771386807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">antagonists</tok><tok cat="COMMA">,</tok></cons> <cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="JJ">ester-induced</tok> <tok cat="NN">up-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HB-EGF</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
771386808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Pretreatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">pertussis</tok> <tok cat="NN">toxin</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">functions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PAF</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">cholera</tok> <tok cat="NN">toxin</tok></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NN">effect</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
771386809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Pyrrolidine</tok> <tok cat="NN">dithiocarbamate</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="ADVP"><tok cat="RB">markedly</tok></cons> <cons cat="VP"><tok cat="VBD">reduced</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">PAF-stimulated</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">up-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HB-EGF</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
771386810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potential</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PAF</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HB-EGF</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><tok cat="NN">evidence</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">occur</tok> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
771851901@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" id="i5" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="NN">receptor</tok></cons> <cons cat="NP" ref="i40" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">TCR</tok></cons> <cons cat="NP" ref="i40" null="RNR"></cons></cons><cons cat="NP" id="i40"><tok cat="NNS">signals</tok></cons></cons> <cons cat="VP"><tok cat="VBP">synergize</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="JJ">B-mediated</tok> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
771851902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Previous</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i6" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-1R</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COLON">-</tok> <cons cat="NP" ref="i31" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP" id="i31"><tok cat="JJ">TCR-initiated</tok> <tok cat="NNS">signals</tok></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">interact</tok> <cons cat="ADVP"><tok cat="RB">synergistically</tok></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">increase</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">rate</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">several</tok> <cons cat="NP"><tok cat="NN">lymphokine</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">lymphokine</tok> <tok cat="NN">receptor</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">competence</tok> <tok cat="NN">phase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok> <tok cat="NN">program</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">helper</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@43@@oe@16-12-2010
771851903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">report</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">describe</tok> <cons cat="SBAR"><cons cat="WHADVP" id="i30"><tok cat="WRB">how</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">signals</tok></cons> <cons cat="VP"><tok cat="VBN">initiated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">I</tok> <tok cat="NN">IL-1R</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">interact</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" id="i10"><cons cat="NP"><tok cat="NNS">signals</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">antigen</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="ADVP"><tok cat="RB">synergistically</tok></cons> <cons cat="VP"><tok cat="VB">augment</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transactivating</tok> <tok cat="NNS">properties</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i30" null="T"></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
771851904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">synergistic</tok> <cons cat="NP"><tok cat="NN">antigen</tok> <tok cat="NN">receptor</tok></cons> <tok cat="JJ">initiated</tok> <tok cat="NNS">signals</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">because</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">mimicked</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phorbol</tok> <tok cat="NN">ester</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">12-O-tetradecanoylphorbol-13-acetate</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">calcium</tok> <tok cat="NNS">ionophores</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" syn="COOD" role="PRD"><cons cat="ADJP"><tok cat="NN">staurosporine</tok> <tok cat="JJ">sensitive</tok></cons> <tok cat="CC">but</tok> <cons cat="ADJP"><tok cat="NN">cyclosporine</tok> <tok cat="JJ">resistant</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
771851905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Gel</tok> <tok cat="NN">shift</tok> <tok cat="NNS">analyses</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i13" role="SBJ"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="JJ">nuclear</tok> <tok cat="NN">translocation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">primarily</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">IL-1</tok></cons></cons> <cons cat="CONJP"><tok cat="RB">rather</tok> <tok cat="IN">than</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">antigen</tok> <tok cat="NN">receptor</tok></cons> <tok cat="NNS">signals</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
771851906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Western</tok> <tok cat="NN">blot</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">phosphorylation</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NNS">analyses</tok></cons></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">synergistic</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="JJ">functional</tok> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">independent</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MAD3</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="NN">-NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">dissociation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytosol</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i14" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="JJ">nuclear</tok> <tok cat="NN">translocation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
771851907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">IL-1-induced</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">DNA</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">localization</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">transient</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">prolonged</tok> <cons cat="PP" syn="COOD"><tok cat="CC">either</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">an</tok> <tok cat="NN">antigen</tok> <tok cat="JJ">receptor-initiated</tok> <tok cat="NN">signal</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inhibiting</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">synthesis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
771851908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-1</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="JJ">nuclear</tok> <tok cat="NN">translocation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">synthesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">protein</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons> <cons cat="ADJP"><tok cat="JJ">responsible</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">terminating</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B-DNA</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
771851909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i15" role="SBJ"><tok cat="NN">Antigen</tok> <tok cat="NN">receptor</tok> <tok cat="NNS">signals</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">prolong</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B-DNA</tok> <tok cat="NN">interaction</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">probably</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i15" role="SBJ" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">functionally</tok></cons> <cons cat="VP"><tok cat="VBG">antagonizing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">IL-1-induced</tok> <tok cat="NN">synthesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">protein</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons> <cons cat="ADJP"><tok cat="JJ">responsible</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transient</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B-DNA</tok> <tok cat="NN">interaction</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">consequently</tok></cons> <cons cat="ADVP"><tok cat="RB">synergistically</tok></cons> <cons cat="VP"><tok cat="VBP">enhance</tok> <cons cat="NP"><tok cat="JJ">IL-1-induced</tok> <tok cat="NN">NF-kappa</tok> <tok cat="JJ">B-dependent</tok> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
771924801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">A</tok> <cons cat="ADJP"><tok cat="RB">newly</tok> <tok cat="JJ">established</tok></cons> <cons cat="NP"><tok cat="JJ">megakaryoblastic/erythroid</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">differentiates</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">red</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">erythropoietin</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">produces</tok> <cons cat="NP"><tok cat="JJ">platelet-like</tok> <tok cat="NNS">particles</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@16-12-2010
771924802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNP">August</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">1992</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">established</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">leukemic</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NS-Meg</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">patient</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">megakaryoblastic</tok> <tok cat="NN">transformation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Philadelphia</tok> <tok cat="JJ">chromosome-positive</tok> <tok cat="JJ">chronic</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">leukemia</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@16-12-2010
771924803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">NS-Meg</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">positive</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">alpha-naphthyl</tok> <tok cat="NN">acetate</tok> <tok cat="NN">esterase</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">periodic</tok> <tok cat="NN">acid-Schiff</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PAS</tok></cons><tok cat="RRB">)</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">staining</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><tok cat="NN">surface</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD4</tok></cons><cons cat="NP" null="QSTN"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD7</tok></cons><cons cat="NP" null="QSTN"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD13</tok></cons><cons cat="NP" null="QSTN"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD34</tok></cons><cons cat="NP" null="QSTN"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD41a</tok></cons><cons cat="NP" null="QSTN"></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">glycophorin</tok> <tok cat="NN">A</tok> <tok cat="NNS">antigens</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
771924804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Ultrastructurally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">alpha-granules</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">demarcation</tok> <tok cat="NNS">membranes</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">platelet</tok> <tok cat="NN">peroxidase</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
771924805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">NS-Meg</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">spontaneously</tok></cons> <cons cat="VP"><tok cat="VBD">produced</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">platelet-like</tok> <tok cat="NNS">particles</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">alpha-granules</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">mitochondria</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">dense</tok> <tok cat="NNS">bodies</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><tok cat="NN">platelet</tok> <tok cat="NN">production</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
771924806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Erythropoietin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Epo</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">granulocyte/macrophage</tok> <tok cat="NN">colony</tok> <tok cat="NN">stimulating</tok> <tok cat="NN">factor</tok></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GM-CSF</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="CD">3</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-3</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">promoted</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">growth</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NS-Meg</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
771924807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Phorbol-12-myristate-13-acetate</tok></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD41a</tok></cons> <cons cat="NP" ref="i25" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CD61</tok> <cons cat="NP" ref="i25" null="RNR"></cons></cons><cons cat="NP" id="i25"><tok cat="NNS">antigens</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
771924808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Ten-day</tok> <tok cat="NN">exposure</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">Epo</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="NNS">erythroblasts</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">red</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
771924809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <cons cat="NP"><tok cat="JJ">benzidine-positive</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">positive</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">hemoglobin</tok></cons> <tok cat="NN">F</tok> <tok cat="NN">staining</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
771924810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Untreated</tok> <cons cat="NP"><tok cat="NN">NS-Meg</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP"><tok cat="NN">mRNA</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Epo</tok> <tok cat="NN">receptor</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EpoR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">alpha</tok> <tok cat="CD">1</tok><cons cat="NP" ref="i27" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">alpha</tok> <tok cat="CD">2</tok> <cons cat="NP" ref="i27" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">gamma</tok> <cons cat="NP" ref="i27" null="RNR"></cons></cons><cons cat="NP" id="i27"><tok cat="NN">globin</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
771924811@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NS-Meg</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">undergo</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">terminal</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">megakaryocytic</tok></cons> <cons cat="NP" ref="i28" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">erythroid</tok></cons> <cons cat="NP" ref="i28" null="RNR"></cons></cons><cons cat="NP" id="i28"><tok cat="NNS">lineages</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
771924812@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons> <cons cat="VP"><tok cat="MD">should</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">very</tok> <tok cat="JJ">useful</tok></cons> <tok cat="NN">tool</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">investigation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">megakaryocytic</tok></cons> <cons cat="NP" ref="i29" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">erythroid</tok></cons> <cons cat="NP" ref="i29" null="RNR"></cons></cons><cons cat="NP" id="i29"><tok cat="NN">maturation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
771993701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Identification</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">purification</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">Stat</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">interleukin-2</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
771993702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">key</tok> <cons cat="NP"><tok cat="NN">cytokine</tok></cons></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="NN">IL-2</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@11@@oe@16-12-2010
771993703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="VBG">Following</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">various</tok> <tok cat="NNS">chains</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">transcriptionally</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
771993704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">turn</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><tok cat="VBN">secreted</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">serves</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">stimulate</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">proliferation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
771993705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Several</tok> <tok cat="JJ">recent</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Jak</tok> <tok cat="NNS">kinases</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
771993706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Following</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">lead</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">set</tok> <cons cat="PRT"><tok cat="RP">out</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">identify</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
771993707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="JJ">Human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">contain</tok> <cons cat="NP"><tok cat="JJ">several</tok> <tok cat="JJ">IL-2-inducible</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NNS">activities</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
771993708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="JJ">Similar</tok> <tok cat="NNS">activities</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="VBN">transformed</tok> <tok cat="JJ">human</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NN">line</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">termed</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NN">YT</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
771993709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">purified</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">activities</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">principal</tok> <cons cat="NP"><tok cat="JJ">IL-2-inducible</tok> <tok cat="NN">component</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">bears</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">significant</tok> <tok cat="NN">relatedness</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">prolactin-induced</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">first</tok></cons> <tok cat="VBN">identified</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">sheep</tok> <tok cat="JJ">mammary</tok> <tok cat="NN">gland</tok> <tok cat="NN">tissue</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
771993710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">hypothesize</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">protein</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">designated</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NN">hStat5</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">helps</tok> <cons cat="S"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VB">govern</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">biological</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
771993801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">shared</tok> <tok cat="NN">receptor</tok> <tok cat="NNS">motifs</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">common</tok> <tok cat="NN">Stat</tok> <tok cat="NNS">proteins</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">generation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">cytokine</tok></cons> <cons cat="NP"><tok cat="NN">pleiotropy</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">redundancy</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">IL-2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-7</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-13</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-15</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@30@@oe@16-12-2010
771993802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">understand</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NNS">bases</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><tok cat="NN">cytokine</tok> <cons cat="NP"><tok cat="NN">redundancy</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">pleiotropy</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Stat</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">PBLs</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">cytokines</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">shared</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">distinct</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">actions</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@33@@oe@16-12-2010
771993803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Interleukin-2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-2</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok></cons> <cons cat="VP"><tok cat="VBD">activated</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">Stat5</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">fresh</tok> <cons cat="NP"><tok cat="NN">PBL</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Stat3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Stat5</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">preactivated</tok> <tok cat="NN">PBL</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
771993804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">IL-7</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-15</tok></cons></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">feature</tok></cons> <cons cat="VP"><tok cat="VBN">explained</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">existence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">similar</tok> <cons cat="NP"><tok cat="JJ">tyrosine-phosphorylated</tok> <tok cat="NNS">motifs</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">cytoplasmic</tok> <tok cat="NNS">domains</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">beta</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-7R</tok></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">serve</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">docking</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">Stat</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
771993805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-13</tok></cons> <cons cat="VP"><tok cat="VBD">Induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">finding</tok></cons> <cons cat="VP"><tok cat="VBN">explained</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="PRPP">our</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBG">implicating</tok> <cons cat="NP"><tok cat="NN">IL-4R</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">shared</tok> <tok cat="NN">component</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
771993806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">a</tok> <tok cat="JJ">single</tok> <cons cat="NP"><tok cat="NN">cytokine</tok></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">combinations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Stat</tok> <tok cat="NNS">proteins</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">different</tok></cons> <cons cat="ADJP"><tok cat="JJ">physiological</tok></cons></cons> <tok cat="NNS">conditions</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">distinct</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <cons cat="NP"><tok cat="NN">Stat</tok> <tok cat="NN">protein</tok></cons></cons><cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
772008501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">aspirin-like</tok> <tok cat="NNS">drugs</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@12@@oe@16-12-2010
772008502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Aspirin-like</tok> <tok cat="NNS">drugs</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ALD</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">calcium</tok> <tok cat="NN">mobilization</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">essential</tok> <tok cat="NN">component</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBP">do</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">biosynthesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@26@@oe@16-12-2010
772008503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">understand</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">extent</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="TO">to</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">ALD</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">mimic</tok> <cons cat="NP"><tok cat="JJ">mitogenic</tok> <tok cat="NN">stimulation</tok></cons><cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">studied</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">cytoplasmic</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">nuclear</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">signaling</tok> <tok cat="NNS">steps</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">ALD-treated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
772008504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">ALD</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">transient</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PKC</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">effect</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">comparison</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">anti-CD3</tok> <tok cat="NNS">antibodies</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons></cons> <tok cat="CC">nor</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">PCL</tok> <tok cat="NN">gamma</tok> <tok cat="CD">1</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
772008505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="JJ">ALD-induced</tok> <cons cat="NP"><tok cat="NN">calcium</tok> <tok cat="NN">mobilization</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PKC</tok></cons> <tok cat="NN">activation</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">independent</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">lack</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">herbimycin</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">tyrosine-protein</tok> <tok cat="JJ">kinase-specific</tok> <tok cat="NN">inhibitor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
772008506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">detected</tok> <cons cat="NP"><tok cat="DT">no</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">mRNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">ALD-treated</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">nuclei</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">contain</tok> <cons cat="NP"><cons cat="NP" id="i33"><tok cat="NNS">proteins</tok></cons> <cons cat="ADJP"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i33" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok> <cons cat="NP"><tok cat="JJ">regulatory</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="NP" ref="i30" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD" id="i30"><cons cat="NP"><tok cat="NN">NFAT</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
772008507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">These</tok> <tok cat="NN">binding</tok> <tok cat="NNS">activities</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">only</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
772008508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">depended</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">calcium</tok> <tok cat="NN">mobilization</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PKC</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
772008509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">ALD</tok></cons> <cons cat="VP"><tok cat="VBP">cause</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">transient</tok></cons> <tok cat="CC">but</tok> <cons cat="ADJP"><tok cat="JJ">significant</tok></cons></cons> <tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">transmembrane</tok> <tok cat="NN">signaling</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">some</tok> <tok cat="NNS">events</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">anti-CD3</tok> <tok cat="NNS">antibodies</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">ALD</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
772008510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">signal</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">transmitted</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">several</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
772008511@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i11" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">ALD</tok> <tok cat="NN">stimulus</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">causing</tok> <cons cat="NP"><tok cat="JJ">complete</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
772174701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Functional</tok> <tok cat="NNS">roles</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok> <tok cat="VBN">footprinted</tok> <tok cat="NN">DNA</tok> <tok cat="NNS">motifs</tok></cons></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">alpha-globin</tok> <tok cat="NN">enhancer</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
772174702@GENIA Treebank@formal@@1@S@<cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">Erythroid</tok> <tok cat="NN">lineage</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">developmental</tok> <tok cat="NN">stage</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">specificities</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@7@@oe@16-12-2010
772174703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="JJ">Transcriptional</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">alpha-like</tok> <tok cat="NN">globin</tok> <tok cat="NNS">genes</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">embryonic</tok> <tok cat="NN">zeta</tok> <tok cat="CD">2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="NN">alpha</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NN">development</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">distal</tok> <tok cat="NN">enhancer</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">HS-40</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@28@@oe@16-12-2010
772174704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Previous</tok> <tok cat="JJ">protein-DNA</tok> <tok cat="NN">binding</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HS-40</tok></cons> <cons cat="VP"><tok cat="VBZ">consists</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">multiple</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">binding</tok> <tok cat="NNS">motifs</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" id="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">occupied</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">erythroid</tok> <tok cat="NN">lineage-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">developmental</tok> <tok cat="JJ">stage-specific</tok></cons></cons> <tok cat="NN">manner</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
772174705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP"><tok cat="RB">systematically</tok></cons> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">functional</tok> <tok cat="NNS">roles</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NN">factor</tok> <tok cat="NN">binding</tok> <tok cat="NNS">motifs</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HS-40</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">site-directed</tok> <tok cat="NN">mutagenesis</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">expression</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">assay</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">cultures</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
772174706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="CD">Three</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NN">HS-40</tok> <tok cat="NN">enhancer</tok> <tok cat="NNS">motifs</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">5'NF-E2/AP1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">GT</tok> <tok cat="CD">II</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">GATA-1(c)</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="ADVP"><tok cat="RB">positively</tok></cons> <cons cat="VP"><tok cat="VBP">regulate</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">zeta</tok> <tok cat="NN">2-globin</tok> <tok cat="NN">promoter</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">embryonic/fetal</tok> <tok cat="JJ">erythroid</tok> <tok cat="NN">K562</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="NN">alpha-globin</tok> <tok cat="NN">promoter</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="JJ">erythroid</tok> <tok cat="NN">MEL</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
772174707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">On</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">other</tok> <tok cat="NN">hand</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">3'NF-E2/AP1</tok> <tok cat="NN">motif</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">exert</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">positive</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">negative</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="JJ">regulatory</tok> <tok cat="NNS">effects</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">zeta</tok> <tok cat="NN">2-globin</tok> <tok cat="NN">promoter</tok></cons> <tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">K562</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i28" role="SBJ"><tok cat="DT">this</tok> <tok cat="JJ">dual</tok> <tok cat="NN">function</tok></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="S"><cons cat="NP" ref="i28" id="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">modulated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">differential</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">ubiquitous</tok> <tok cat="NN">AP1</tok> <tok cat="NNS">factors</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">erythroid-enriched</tok> <tok cat="NN">NF-E2</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@50@@oe@16-12-2010
772174708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Mutation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GATA-1(d)</tok> <tok cat="NN">motif</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">exhibits</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="JJ">erythroid-specific</tok> <tok cat="JJ">genomic</tok> <tok cat="NN">footprint</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">decreases</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HS-40</tok> <tok cat="NN">enhancer</tok></cons> <tok cat="NN">function</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">dimethyl</tok> <tok cat="JJ">sulfoxide-induced</tok> <tok cat="NN">MEL</tok> <tok cat="NNS">cells</tok></cons></cons> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">K562</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
772174709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">defined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">roles</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">different</tok> <cons cat="NP"><tok cat="NN">HS-40</tok> <tok cat="NNS">motifs</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
772174710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">remarkable</tok> <tok cat="NN">correlation</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">genomic</tok> <tok cat="NN">footprinting</tok> <tok cat="NNS">data</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mutagenesis</tok> <tok cat="NNS">results</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i15" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">erythroid</tok> <tok cat="NN">lineage-</tok> <cons cat="ADJP" null="QSTN"></cons><cons cat="NP" ref="i25" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">developmental</tok> <tok cat="JJ">stage-specific</tok></cons> <cons cat="NP" ref="i25" null="RNR"></cons></cons><cons cat="NP" id="i25"><tok cat="NN">regulation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">alpha-like</tok> <tok cat="NN">globin</tok> <tok cat="NNS">promoters</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">indeed</tok></cons> <cons cat="VP"><tok cat="VBN">modulated</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">stable</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
772188501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Interleukin</tok> <tok cat="LRB">(</tok><tok cat="NN">IL</tok><tok cat="RRB">)</tok><tok cat="CD">-10</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@16-12-2010
772188502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">IL-10</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons> <cons cat="VP"><tok cat="VBP">suppress</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok></cons> <tok cat="NN">synthesis</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">different</tok> <tok cat="NNS">mechanisms</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
772188503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRPP">Our</tok> <tok cat="JJ">previous</tok> <tok cat="NNS">studies</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">interleukin</tok> <tok cat="LRB">(</tok><tok cat="NN">IL</tok><tok cat="RRB">)</tok><tok cat="CD">-10</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lipopolysaccharide</tok> <tok cat="LRB">(</tok><tok cat="NN">LPS</tok><tok cat="RRB">)</tok><tok cat="JJ">-stimulated</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NNS">cytokines</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="NN">beta</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-6</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-8</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="LRB">(</tok><tok cat="NN">TNF</tok><tok cat="RRB">)</tok><tok cat="NN">-alpha</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">blocking</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@45@@oe@16-12-2010
772188504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EMSA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok><cons cat="S"><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">LPS</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">TNF</tok> <tok cat="NN">alpha</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">IL-10</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NN">cytokine</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@50@@oe@16-12-2010
772188505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">Several</tok> <tok cat="JJ">other</tok></cons> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-IL-6</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">AP-2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">GR</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CREB</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Oct-1</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Sp-1</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">affected</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">IL-10</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
772188506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">This</tok> <tok cat="JJ">selective</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">IL-10</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="UCP" syn="COOD"><cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">dose-dependent</tok> <tok cat="NN">manner</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">correlates</tok> <cons cat="ADVP"><tok cat="RB">well</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-10</tok></cons><tok cat="POS">'s</tok> <cons cat="NP"><tok cat="NN">cytokine</tok></cons> <tok cat="NN">synthesis</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">terms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NNS">kinetics</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">dose</tok> <tok cat="NN">responsiveness</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
772188507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">compounds</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">tosylphenylalanyl</tok> <tok cat="NN">chloromethyl</tok> <tok cat="NN">ketone</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">pyrrolidinedithiocarbamate</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="ADVP"><tok cat="RB">selectively</tok></cons> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">block</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">LPS-stimulated</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
772188508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">Taken</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">together</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">IL-10</tok></cons> <tok cat="NN">suppression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
772188509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">IL-4</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">another</tok> <cons cat="NP"><tok cat="NN">cytokine</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="NN">accumulation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">shows</tok> <cons cat="NP"><tok cat="JJ">little</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">LPS-induced</tok> <cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
772188510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="RB">Further</tok> <tok cat="NN">examination</tok></cons> <cons cat="VP"><tok cat="VBZ">reveals</tok> <cons cat="SBAR"><tok cat="IN">that</tok><cons cat="S"><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">unlike</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">IL-4</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">enhances</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">mRNA</tok></cons> <tok cat="NN">degradation</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">suppress</tok> <cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
772188511@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">IL-10</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons> <cons cat="VP"><tok cat="VBP">inhibit</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok></cons> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">different</tok> <tok cat="NNS">mechanisms</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
772778701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Identification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">major</tok> <cons cat="NP"><tok cat="JJ">positive</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">element</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <tok cat="JJ">5'</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">zeta-globin</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
772778702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">zeta-globin</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">studied</tok> <cons cat="NP" ref="i5" null="NONE"></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">series</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">zeta-globin</tok> <tok cat="NN">promoter</tok> <tok cat="NN">deletion</tok> <tok cat="NNS">constructs</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i9" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">drive</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">luciferase</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="RB">transiently</tok> <tok cat="VBN">transfected</tok> <tok cat="JJ">human</tok> <tok cat="NN">erythroleukemia</tok> <tok cat="NNS">cells</tok></cons></cons><cons cat="ADVP" ref="i9" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@16-12-2010
772778703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NNS">promoters</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP"><tok cat="NNS">enhancers</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NNS">enhancers</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">beta-globin</tok> <tok cat="NN">locus</tok> <tok cat="NN">control</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">alpha-globin</tok> <tok cat="NN">HS-40</tok> <tok cat="NN">enhancer</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
772778704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i7"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">transfected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">K562</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i17"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i17" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP"><tok cat="NN">zeta-globin</tok></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i7" null="T"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="JJ">comparable</tok> <tok cat="NNS">amounts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">activity</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="CD">-557</tok> <tok cat="CC">and</tok> <tok cat="CD">-417</tok> <tok cat="NN">zeta-luciferase</tok> <tok cat="NNS">constructs</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">alpha-luciferase</tok> <tok cat="NNS">constructs</tok></cons></cons></cons></cons></cons> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i11"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" syn="COOD" id="i12" role="SBJ"><cons cat="NP"><tok cat="DT">no</tok> <cons cat="NP"><tok cat="NNS">enhancers</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">alpha-globin</tok> <tok cat="NN">locus</tok> <tok cat="NNS">enhancers</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">used</tok><cons cat="NP" ref="i12" null="NONE"></cons><cons cat="ADVP" ref="i11" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
772778705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i13"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" id="i14" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">constructs</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">transfected</tok> <cons cat="NP" ref="i14" null="NONE"></cons><cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">OCIM1</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i15"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i15" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">do</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">express</tok> <cons cat="NP"><tok cat="NN">zeta-globin</tok></cons></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i13" null="T"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">zeta-globin</tok> <tok cat="NNS">promoters</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><cons cat="ADVP"><tok cat="IN">at</tok> <tok cat="RB">best</tok></cons> <cons cat="NP"><tok cat="CD">20</tok><tok cat="NN">%</tok></cons> <tok cat="IN">as</tok> <tok cat="JJ">active</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">alpha-globin</tok> <tok cat="NNS">promoters</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
772778706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i16"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" id="i27" role="SBJ"><cons cat="NP"><tok cat="NNS">sequences</tok> <tok cat="IN">from</tok> <tok cat="CD">-417</tok> <tok cat="TO">to</tok> <tok cat="CD">-207</tok> <tok cat="JJ">5'</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">zeta-globin</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">cap</tok> <tok cat="NN">site</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">deleted</tok><cons cat="NP" ref="i27" null="NONE"></cons><cons cat="ADVP" ref="i16" null="T"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i18" role="SBJ"><cons cat="NP"><cons cat="QP"><tok cat="IN">up</tok> <tok cat="TO">to</tok> <tok cat="CD">95</tok></cons><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">zeta-globin</tok> <tok cat="NN">promoter</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">lost</tok> <cons cat="NP" ref="i18" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">K562</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
772778707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Reinsertion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">sequences</tok></cons></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">zeta-luciferase</tok> <tok cat="NNS">constructs</tok></cons> <cons cat="VP"><tok cat="VBG">missing</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">-417</tok> <tok cat="TO">to</tok> <tok cat="CD">-207</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="VP"><tok cat="VBP">lack</tok> <cons cat="NP"><tok cat="JJ">classical</tok> <tok cat="NN">enhancer</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
772778708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Point</tok> <tok cat="NN">mutation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok> <tok cat="NN">site</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="CD">-230</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">reduced</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="CD">37</tok><tok cat="NN">%</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
772778709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Point</tok> <tok cat="NN">mutation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">CCACC</tok> <tok cat="NN">site</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="CD">-240</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">effect</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
772778710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons> <cons cat="VP"><tok cat="VBD">indicated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="CD">-230</tok> <tok cat="NN">GATA-1</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">relatively</tok> <tok cat="JJ">low</tok></cons> <tok cat="NN">affinity</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
772778711@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="COMP" null="ZERO"></cons><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">strong</tok> <cons cat="NP"><tok cat="JJ">positive-acting</tok> <tok cat="NN">element</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">-417</tok> <tok cat="CC">and</tok> <tok cat="CD">-207</tok> <tok cat="NN">bp</tok> <tok cat="JJ">5'</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">zeta-globin</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">cap</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">necessary</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">high-level</tok> <tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">K562</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
772778712@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">element</tok></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">GATA-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">additional</tok> <tok cat="JJ">unknown</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">maximal</tok> <tok cat="NN">activity</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
773036401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <cons cat="NP"><tok cat="NN">C-alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><tok cat="NN">-epsilon</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><tok cat="NN">-zeta</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@16-12-2010
773036402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">multiple</tok> <tok cat="NNS">isotypes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PKC</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="S" func="ADV"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">well</tok></cons> <cons cat="VP"><tok cat="VBN">accepted</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">PKCs</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="JJ">little</tok> <cons cat="PP" ref="i6" null="ICH"></cons></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="NP" ref="i5" null="NONE"></cons></cons></cons><cons cat="PP" id="i6"><tok cat="IN">about</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">individual</tok> <cons cat="NP"><tok cat="NN">PKC</tok> <tok cat="NNS">isotypes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@40@@oe@16-12-2010
773036403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">address</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">issue</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" syn="COOD" id="i8" role="SBJ"><cons cat="ADJP"><tok cat="RB">mutationally</tok> <tok cat="JJ">active</tok></cons> <cons cat="NP"><tok cat="NN">PKC-alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><tok cat="NN">-epsilon</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><tok cat="NN">-zeta</tok></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">transfected</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">consequences</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">determined</tok><cons cat="NP" ref="i9" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
773036404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">p21ras</tok></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">essential</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
773036405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Accordingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="RB">constitutively</tok> <tok cat="JJ">active</tok> <tok cat="NNS">PKCs</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">mutationally</tok> <tok cat="VBN">activated</tok></cons> <cons cat="NP"><tok cat="NN">p21ras</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
773036406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">PKC-epsilon</tok></cons> <tok cat="CC">and</tok><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJR">lesser</tok> <tok cat="NN">extent</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PKC-alpha</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">-zeta</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <cons cat="NP"><tok cat="NN">factor</tok> <tok cat="IN">of</tok> <tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-AT-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
773036407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">ability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PKC-epsilon</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-AT-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">similar</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">stimulatory</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="RB">constitutively</tok> <tok cat="VBN">activated</tok> <tok cat="NN">p21ras</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
773036408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i11" role="SBJ"><cons cat="NP"><tok cat="NN">PKC-epsilon</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PKC-alpha</tok></cons> <tok cat="CC">nor</tok> <cons cat="NP"><tok cat="JJ">activated</tok> <cons cat="NP"><tok cat="NN">p21ras</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-KB</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
773036409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Phorbol</tok> <tok cat="NNS">esters</tok></cons> <cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD69</tok></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="NN">none</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">PKC</tok> <tok cat="NNS">isotypes</tok></cons></cons> <cons cat="VP"><tok cat="VBN">tested</tok> <cons cat="NP" null="NONE"></cons></cons></cons></cons></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">effect</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
773036410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i13" role="SBJ"><tok cat="VBN">Activated</tok> <cons cat="NP"><tok cat="NN">Src</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p21ras</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i13" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD69</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
773036411@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">selective</tok> <tok cat="NNS">functions</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">different</tok> <cons cat="NP"><tok cat="NN">PKC</tok> <tok cat="NNS">isotypes</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
773036412@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBG">comparing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">Ras</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">PKC</tok> <tok cat="NNS">mutants</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">PKC-alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p21ras</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">PKC-epsilon</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <tok cat="RB">not</tok> <cons cat="ADJP"><tok cat="JJ">positioned</tok> <cons cat="ADVP"><tok cat="RB">linearly</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">single</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
773062401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-E1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">YY1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">newly</tok> <tok cat="VBN">identified</tok></cons> <tok cat="NN">element</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NN">exon</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">DR</tok> <tok cat="NN">alpha</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@26@@oe@16-12-2010
773062402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <cons cat="ADJP"><tok cat="RB">previously</tok> <tok cat="JJ">unrecognized</tok></cons> <tok cat="NN">element</tok><cons cat="SBAR" ref="i4" null="ICH"></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="ADVP"><tok cat="RB">downstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">start</tok> <tok cat="NN">site</tok> <tok cat="IN">of</tok> <tok cat="NN">transcription</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <cons cat="NP"><tok cat="NN">exon</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">DR</tok> <tok cat="NN">alpha</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">defined</tok> <cons cat="NP" ref="i5" null="NONE"></cons></cons></cons></cons><cons cat="SBAR" id="i4"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">enhances</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons> <cons cat="NP" func="ADV"><tok cat="IN">up</tok> <tok cat="TO">to</tok> <tok cat="JJ">eightfold</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">position-dependent</tok> <tok cat="NN">manner</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@38@@oe@16-12-2010
773062403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Mutations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NN">site</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">abolished</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">extract</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">decreased</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">DR</tok> <tok cat="NN">alpha</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">basal</tok> <tok cat="NN">level</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
773062404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="JJ">Significant</tok> <tok cat="NN">sequence</tok> <tok cat="NN">homology</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <cons cat="NP"><tok cat="NN">element</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DNA</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">DR</tok> <tok cat="NN">beta</tok><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><tok cat="NN">DP</tok> <cons cat="NP"><tok cat="NN">alpha</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">-beta</tok><cons cat="NP" ref="i20" null="RNR"></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">DQ</tok> <tok cat="NN">alpha</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">genes</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">always</tok></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="ADVP"><tok cat="RB">downstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">start</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
773062405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons> <cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">DR</tok> <tok cat="NN">alpha</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">DP</tok> <tok cat="NN">alpha</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">element</tok></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">element</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">DQ</tok> <tok cat="NN">alpha</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
773062406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-E1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">YY1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
773062407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" id="i10"><tok cat="DT">This</tok> <tok cat="NN">protein</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Ig</tok> <tok cat="NN">kappa</tok> <tok cat="JJ">3'</tok> <tok cat="NN">enhancer</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Ig</tok> <tok cat="JJ">heavy</tok> <tok cat="NN">chain</tok> <tok cat="NN">mu</tok> <tok cat="NN">E1</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">quite</tok> <tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
773801301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Mouse</tok> <tok cat="NN">interleukin-2</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok><tok cat="PERIOD">.</tok></cons></cons>@@@@1@7@@oe@16-12-2010
773801302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Interleukin-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">interleukin-2</tok></cons></cons> <cons cat="VP"><tok cat="VBP">control</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="JJ">distinct</tok> <cons cat="NP"><tok cat="JJ">cis-acting</tok> <tok cat="NNS">elements</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
773801303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons> <cons cat="VP"><tok cat="VBP">control</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha</tok> <tok cat="LRB">(</tok><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok><tok cat="RRB">)</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">CD4-CD8-</tok> <tok cat="JJ">murine</tok> <tok cat="NN">T</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NNS">precursors</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@16-12-2010
773801304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">map</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">cis-acting</tok> <tok cat="NNS">elements</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">mediate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="NN">responsiveness</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">mouse</tok> <tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">thymic</tok> <tok cat="JJ">lymphoma-derived</tok> <tok cat="NN">hybridoma</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PC60</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
773801305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">IL-1</tok></cons> <tok cat="CC">+</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">biphasic</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
773801306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-1</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">rapid</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="NN">protein</tok> <tok cat="JJ">synthesis-independent</tok></cons></cons> <tok cat="NN">appearance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok> <tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" id="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">inhibitors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
773801307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">primes</tok> <cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">become</tok> <cons cat="ADJP" role="PRD"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="JJ">responsive</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">thereby</tok></cons> <cons cat="VP"><tok cat="VBZ">prepares</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">second</tok> <tok cat="NN">phase</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-2</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">100-fold</tok> <tok cat="JJ">further</tok></cons> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok> <tok cat="NNS">transcripts</tok></cons></cons><cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
773801308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Transient</tok> <tok cat="NN">transfection</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NNS">elements</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">promoter-proximal</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">IL-1</tok></cons> <tok cat="NN">responsiveness</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RBS">most</tok> <tok cat="RB">importantly</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="VP"><tok cat="VBN">conserved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">human</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">mouse</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
773801309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="NN">responsiveness</tok><tok cat="COMMA">,</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">other</tok> <tok cat="NN">hand</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">depends</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">78-nucleotide</tok> <tok cat="NN">segment</tok></cons></cons> <cons cat="ADVP"><cons cat="NP"><tok cat="CD">1.3</tok> <tok cat="NNS">kilobases</tok></cons> <tok cat="RB">upstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">major</tok> <tok cat="NN">transcription</tok> <tok cat="NN">start</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
773801310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">segment</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">functions</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">IL-2-inducible</tok> <tok cat="NN">enhancer</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">lies</tok> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">region</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">becomes</tok> <cons cat="ADJP" role="PRD"><cons cat="NP"><tok cat="NN">DNase</tok> <tok cat="CD">I</tok></cons> <tok cat="JJ">hypersensitive</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">normal</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="SBAR"><cons cat="WHPP" id="i31"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">induced</tok><cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP" ref="i31" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
773801311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="NN">responsiveness</tok></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok> <tok cat="JJ">distinct</tok> <cons cat="NP"><tok cat="NNS">elements</tok></cons></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">enhancer</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
773801312@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">Two</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">potential</tok> <tok cat="VBG">binding</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
773956201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Coupling</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">response</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">multiple</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
773956202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">eukaryotic</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">central</tok> <tok cat="NN">role</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">induced</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">many</tok> <tok cat="NNS">aspects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">genetic</tok> <tok cat="NN">program</tok></cons> <cons cat="VP"><tok cat="VBG">mediating</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">normal</tok> <tok cat="NN">T-cell</tok> <cons cat="NP"><tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">growth</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@35@@oe@16-12-2010
773956203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">tightly</tok></cons> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cytoplasmic</tok> <tok cat="NN">compartment</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">inhibitory</tok> <tok cat="NN">subunit</tok></cons></cons> <cons cat="VP"><tok cat="VBN">called</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
773956204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">This</tok> <tok cat="JJ">cytoplasmic</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">phosphorylated</tok></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">degraded</tok></cons> <cons cat="NP" ref="i8" null="NONE"></cons></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">diverse</tok> <tok cat="NN">set</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="JJ">B-inducing</tok> <tok cat="NNS">agents</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NNS">mitogens</tok><tok cat="COMMA">,</tok></cons> <cons cat="NP"><tok cat="JJ">proinflammatory</tok> <tok cat="NNS">cytokines</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NNS">transactivators</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Tax</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
773956205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">explore</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">these</tok> <tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="JJ">alpha-dependent</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">induction</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">novel</tok> <tok cat="NNS">mutants</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">uncouple</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">inhibitory</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">signal-transducing</tok></cons></cons> <tok cat="NNS">functions</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
773956206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Specifically</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="NN">removal</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">N-terminal</tok> <tok cat="CD">36</tok> <tok cat="NN">amino</tok> <tok cat="NNS">acids</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">failed</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">disrupt</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">form</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">latent</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytoplasm</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
773956207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">deletion</tok> <tok cat="NN">mutation</tok></cons> <cons cat="VP"><tok cat="VBD">prevented</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">induced</tok> <tok cat="NN">phosphorylation</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">degradative</tok> <tok cat="NN">loss</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="NN">release</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">Tax-expressing</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
773956208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Alanine</tok> <tok cat="NNS">substitutions</tok></cons> <cons cat="VP"><tok cat="VBN">introduced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NN">serine</tok> <tok cat="NNS">residues</tok></cons> <cons cat="ADJP"><tok cat="JJ">positioned</tok> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <cons cat="NP"><tok cat="JJ">N-terminal</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">yielded</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">constitutive</tok> <tok cat="NNS">repressors</tok></cons> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><cons cat="WHNP" id="i12"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i12" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">escaped</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">Tax-induced</tok> <tok cat="NN">turnover</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><cons cat="WHNP" id="i13"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i13" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">potently</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">immune</tok> <tok cat="NN">activation</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">induction</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="VP"><tok cat="VBN">initiated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">antigen</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">cytokine</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@48@@oe@16-12-2010
773956209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">introduction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">phosphoserine</tok></cons> <cons cat="ADJP"><tok cat="JJ">mimetic</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">rectified</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">functional</tok> <tok cat="NN">defect</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">finding</tok></cons> <cons cat="ADJP"><tok cat="JJ">consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">causal</tok> <tok cat="NN">linkage</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phosphorylation</tok> <tok cat="NN">status</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">proteolytic</tok> <tok cat="NN">stability</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <cons cat="NP"><tok cat="JJ">cytoplasmic</tok> <tok cat="NN">inhibitor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
773956210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Together</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">define</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">critical</tok> <tok cat="NN">signal</tok> <tok cat="NN">response</tok> <tok cat="NN">domain</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i14"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i14" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">coordinately</tok></cons> <cons cat="VP"><tok cat="VBZ">controls</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">biologic</tok> <tok cat="NNS">activities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">viral</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">immune</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NNS">stimuli</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
774203701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok> <cons cat="NP"><tok cat="NN">protease</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="JJ">lymphoid</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
774203702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">rel</tok> <tok cat="NN">oncogene</tok> <tok cat="NN">family</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">enhancing</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="JJ">cellular</tok> <tok cat="NNS">genes</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">receptor</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBN">transcribed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">LTR</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@36@@oe@16-12-2010
774203703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">reported</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HIV-1</tok> <tok cat="NN">protease</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">cleave</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">precursor</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">active</tok> <tok cat="NN">form</tok></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
774203704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons> <cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">protease</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">precursor</tok> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">introducing</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">protease</tok> <tok cat="NN">expression</tok> <tok cat="NN">vector</tok></cons></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
774203705@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="VBN">Increased</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i6" null="NONE"></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">this</tok> <tok cat="VBN">increased</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">specific</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">protease</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
774203706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="JJ">Viral</tok> <tok cat="NN">transcription</tok><tok cat="COMMA">,</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">measured</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="JJ">LTR-CAT</tok> <tok cat="NNS">assays</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">only</tok> <tok cat="RB">slightly</tok></cons> <cons cat="VP"><tok cat="VBN">enhanced</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HIV-protease</tok> <tok cat="JJ">expressing</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i10" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">secretion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="NN">receptor</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">affected</tok><cons cat="NP" ref="i10" null="NONE"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
774203707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">limited</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">HIV</tok> <tok cat="NN">protease</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unlikely</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">significant</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">virus</tok> <tok cat="NN">expression</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">function</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
774255201@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NNS">Neutrophils</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons> <cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">PU.1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Spi-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">Spi-B</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
774255202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">PU.1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Spi-1</tok> <tok cat="NN">oncogene</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Spi-B</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">closely</tok> <tok cat="JJ">related</tok></cons> <tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">ets</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">family</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">sharing</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">similar</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NNS">specificities</tok></cons> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">similar</tok> <cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NNS">domains</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@31@@oe@16-12-2010
774255203@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" syn="COOD" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">PU.1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Spi-B</tok></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">described</tok> <cons cat="NP" ref="i5" null="NONE"></cons></cons></cons><cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">being</tok> <cons cat="ADVP"><tok cat="RB">predominantly</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="ADVP"><tok cat="RB">coordinately</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">macrophages</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">early</tok> <tok cat="JJ">hematopoietic</tok> <tok cat="NNS">stages</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">course</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NNS">neutrophils</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="ADVP"><tok cat="RB">extensively</tok></cons> <cons cat="VP"><tok cat="VBN">investigated</tok><cons cat="NP" ref="i8" null="NONE"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
774255204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="NN">PU.1</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">upregulated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="VBN">purified</tok> <tok cat="JJ">CD34+</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="JJ">multipotential</tok> <tok cat="NN">FDCP-mix</tok> <tok cat="NN">A4</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="NN">PU.1</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">upregulated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">early</tok> <tok cat="NN">event</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">multipotential</tok> <tok cat="NN">progenitor</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
774255205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">PU.1</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">maintained</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="JJ">stable</tok> <tok cat="NNS">levels</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">U937</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HL-60</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">monocytic</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">neutrophilic</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
774255206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="NN">PU.1</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="JJS">highest</tok> <tok cat="NNS">levels</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">neutrophils</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
774255207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">contrast</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">PU.1</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="JJ">significant</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Spi-B</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">only</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="DT">some</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NNS">lines</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">spleen</tok></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBP">are</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNS">neutrophils</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
774255208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADVP"><tok cat="FW">In</tok> <tok cat="FW">vitro</tok></cons> <tok cat="VBN">translated</tok> <cons cat="NP"><tok cat="NN">Spi-B</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">PU.1</tok> <tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NNS">promoters</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">transactivate</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NNS">promoters</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">nonmyeloid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
774255209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">PU.1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Spi-B</tok></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">similar</tok> <tok cat="NN">DNA</tok> <tok cat="NN">control</tok> <tok cat="NNS">elements</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">redundancy</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transactivation</tok> <tok cat="NN">function</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">lack</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">significant</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Spi-B</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">makes</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRP">it</tok></cons> <cons cat="SBAR" ref="i30" null="EXP"></cons></cons><cons cat="ADJP" role="PRD"><tok cat="JJ">unlikely</tok></cons> <cons cat="SBAR" id="i30"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Spi-B</tok></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">significant</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NN">lineage</tok> <tok cat="NN">development</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@49@@oe@16-12-2010
774255210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i13" role="SBJ"><tok cat="NN">PU.1</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">levels</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="CONJP"><tok cat="RB">not</tok> <tok cat="RB">only</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons></cons></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">also</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">neutrophils</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PU.1</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">both</tok> <cons cat="NP"><tok cat="JJ">major</tok> <tok cat="JJ">myeloid</tok> <tok cat="NNS">lineages</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
774323001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">use</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">lymphoblastic</tok> <tok cat="NN">leukemia</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">childhood</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@11@@oe@16-12-2010
774323002@GENIA Treebank@formal@@1@S@<cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">Molecular</tok></cons><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">cellular</tok></cons><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">clinical</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">considerations</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@16-12-2010
774323003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NNS">Glucocorticoids</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">included</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">almost</tok> <tok cat="DT">all</tok></cons> <tok cat="NN">treatment</tok> <tok cat="NNS">regimens</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">childhood</tok> <tok cat="JJ">acute</tok> <tok cat="JJ">lymphoblastic</tok> <tok cat="NN">leukemia</tok></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NNS">decades</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@16-12-2010
774323004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i6" role="SBJ"><cons cat="ADJP"><tok cat="JJ">optimal</tok></cons> <cons cat="NP"><tok cat="NNS">agents</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNS">doses</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and/or</tok> <cons cat="NP"><tok cat="NNS">schedules</tok></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP"><tok cat="RB">yet</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">defined</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">despite</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">extensive</tok></cons> <cons cat="ADJP"><tok cat="JJ">clinical</tok></cons></cons> <tok cat="NN">application</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
774323005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">New</tok> <tok cat="NNS">data</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NNS">pharmacokinetics</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNS">pharmacodynamics</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">molecular</tok> <tok cat="NNS">mechanisms</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">action</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">suggested</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">alternative</tok> <tok cat="NNS">approaches</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">ALL</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
774323006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP"><cons cat="ADJP"><tok cat="JJ">prolonged</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="FW">i.e.</tok></cons> <cons cat="NP"><tok cat="CD">28</tok> <tok cat="NN">day</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="NN">glucocorticoid</tok> <tok cat="NN">therapy</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unnecessary</tok></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">exposure</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">down-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
774323007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Dexamethasone</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">superior</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">prednisone</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">conventional</tok> <tok cat="JJ">equi-effective</tok></cons> <tok cat="NNS">doses</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
774323008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Blast</tok> <tok cat="NN">sensitivity</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">correlates</tok> <cons cat="ADVP"><tok cat="RB">closely</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">sensitivity</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">other</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="RB">putatively</tok> <tok cat="JJ">non-cross-resisting</tok></cons></cons> <tok cat="NNS">agents</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">outcome</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="JJ">multi-agent</tok> <tok cat="NN">therapy</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="UCP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">overlapping</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">action</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">focusing</tok> <cons cat="NP"><tok cat="NN">attention</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">determinants</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">threshold</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
774323009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="VBG">Increasing</tok> <tok cat="NN">success</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">childhood</tok> <tok cat="JJ">acute</tok> <tok cat="JJ">lymphoblastic</tok> <tok cat="NN">leukemia</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">led</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">increasing</tok> <tok cat="NN">awareness</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">avascular</tok> <tok cat="NN">necrosis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">bone</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">potentially</tok> <tok cat="JJ">disabling</tok></cons> <tok cat="NN">sequela</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">therapy</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">especially</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">adolescent</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">young</tok> <tok cat="JJ">adult</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
774462301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Differential</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok> <tok cat="NN">complex</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">restimulation</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">anergy</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
774462302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">CD4+</tok> <tok cat="NN">T-cell</tok> <tok cat="NNS">clones</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TcR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">doses</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">peptide</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">long-lived</tok> <tok cat="NN">state</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">nonresponsiveness</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" id="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">called</tok> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NN">anergy</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@35@@oe@16-12-2010
774462303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">During</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">anergy</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">phenotypically</tok> <tok cat="JJ">similar</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">responding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">immunogenic</tok> <tok cat="NN">stimulus</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
774462304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">amount</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TcR</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">surface</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">downmodulated</tok><cons cat="NP" ref="i8" null="NONE"></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i10" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CD2</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CD25</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">receptors</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">increased</tok><cons cat="NP" ref="i10" null="NONE"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
774462305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i11"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">restimulated</tok><cons cat="NP" ref="i12" null="NONE"></cons><cons cat="ADVP" ref="i11" null="T"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="JJ">anergic</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">fail</tok> <cons cat="S"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">up-regulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">consequence</tok></cons></cons> <cons cat="VP"><tok cat="VBP">do</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">produce</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
774462306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">various</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">appropriate</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">DNA</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
774462307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i33" role="SBJ"><tok cat="NNS">Factors</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" ref="i33" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">nuclei</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="PP" role="PRD"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok> <tok cat="NN">phase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">anergy</tok></cons></cons></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBG">undergoing</tok> <cons cat="NP"><tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
774462308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">pattern</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">restimulated</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pattern</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" id="i13" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i13" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">T-cell-specific</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">beta</tok> <tok cat="NN">chain</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TcR</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
774462309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="VBN">measured</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">TCF-1</tok> <tok cat="NN">site</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">the</tok> <tok cat="JJ">same</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">nuclei</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBG">resting</tok><cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="VBN">activated</tok><cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="VBN">anergized</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
774462310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i16" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">inducible</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NK-kappa</tok> <tok cat="NN">B</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">beta</tok> <tok cat="NN">E2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CD28RC</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i16" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBG">resting</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">twofold</tok> <tok cat="RBR">lower</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">anergized</tok> <cons cat="NP" null="QSTN"></cons></cons></cons></cons><cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
774462311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="JJ">anergic</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><cons cat="NP"><tok cat="RB">approximately</tok> <tok cat="RB">eightfold</tok></cons> <tok cat="JJR">lower</tok></cons> <tok cat="NNS">amounts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-AT</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">member</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">class</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">inducible</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i17"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i17" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">regulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
774462312@GENIA Treebank@formal@@1@S@<cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">ABSTRACT</tok></cons> <cons cat="VP"><tok cat="VBN">TRUNCATED</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">AT</tok> <cons cat="NP"><tok cat="CD">250</tok> <tok cat="NNS">WORDS</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons>@@@@1@7@@oe@16-12-2010
774479901@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Erythropoietin</tok></cons> <cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TAL1/SCL</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NNS">products</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@16-12-2010
774479902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" id="i5"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TAL1</tok> <tok cat="LRB">(</tok><tok cat="CC">or</tok> <tok cat="NN">SCL</tok><tok cat="RRB">)</tok> <tok cat="NN">gene</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">originally</tok></cons> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">a</tok> <tok cat="JJ">recurrent</tok> <tok cat="JJ">chromosomal</tok> <tok cat="NN">translocation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RBS">most</tok> <tok cat="JJ">frequent</tok> <tok cat="JJ">molecular</tok></cons> <tok cat="NN">lesion</tok></cons> <cons cat="VP"><tok cat="VBN">recognized</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="JJ">acute</tok> <tok cat="JJ">lymphoblastic</tok> <tok cat="NN">leukemia</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@34@@oe@16-12-2010
774479903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NNS">products</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">contain</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">basic-helix-loop-helix</tok> <tok cat="NN">motif</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">characteristic</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">large</tok> <tok cat="NN">family</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">canonical</tok> <tok cat="NN">DNA</tok> <tok cat="NN">sequence</tok> <tok cat="NN">CANNTG</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NNS">heterodimers</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
774479904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">TAL1</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NNS">cells</tok></cons> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">chemical-induced</tok> <tok cat="NN">erythroleukemia</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">suggested</tok> <cons cat="COMP" null="ZERO"></cons><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">gene</tok></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">aspects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
774479905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Since</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">terminal</tok> <tok cat="NNS">events</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">erythropoiesis</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">controlled</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glycoprotein</tok> <tok cat="NN">hormone</tok> <tok cat="NN">erythropoietin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Epo</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">expression</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TAL1</tok> <tok cat="NN">gene</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NNS">products</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">affected</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">Epo</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">splenic</tok> <tok cat="NNS">erythroblasts</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">mice</tok></cons> <cons cat="VP"><tok cat="VBN">infected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">anemia-inducing</tok> <tok cat="NN">strain</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Friend</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">FVA</tok> <tok cat="NNS">cells</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@54@@oe@16-12-2010
774479906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="NN">Epo</tok></cons> <cons cat="VP"><tok cat="VBD">elicited</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">rapid</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">dose-related</tok></cons></cons> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">TAL1</tok> <tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBG">increasing</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBG">stabilizing</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <cons cat="NP"><tok cat="NNS">mRNAs</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
774479907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="DT">An</tok> <tok cat="JJ">Epo-inducible</tok> <cons cat="NP"><tok cat="NN">TAL1</tok></cons> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">FVA</tok> <tok cat="NN">cell</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i13"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i13" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">subsequently</tok></cons> <cons cat="VP"><tok cat="VBD">decayed</tok> <cons cat="PP"><tok cat="IN">despite</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">accumulating</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
774479908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="NN">Induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i14" null="NONE"></cons><cons cat="ADVP" adv="TMP"><tok cat="RB">temporally</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Epo-induced</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">TAL1</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
774479909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Epo</tok></cons> <cons cat="VP"><tok cat="VBZ">acts</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">transcriptional</tok></cons> <cons cat="NP" ref="i24" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">posttranscriptional</tok></cons> <cons cat="NP" ref="i24" null="RNR"></cons></cons><cons cat="NP" id="i24"><tok cat="NNS">levels</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TAL1</tok></cons> <tok cat="NN">locus</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Friend</tok> <tok cat="JJ">virus-induced</tok> <tok cat="NNS">erythroblasts</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">establish</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">link</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">Epo</tok></cons> <tok cat="NN">signaling</tok> <tok cat="NNS">mechanisms</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">member</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">family</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">diverse</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lineages</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@16-12-2010
774741701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">retinoblastoma</tok> <tok cat="NN">gene</tok> <tok cat="NN">product</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">negatively</tok></cons> <cons cat="VP"><tok cat="VBZ">regulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">cytomegalovirus</tok> <tok cat="NN">IE2</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@16-12-2010
774741702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">IE2</tok> <tok cat="NN">gene</tok> <tok cat="NN">product</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">cytomegalovirus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HCMV</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">few</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">immediately</tok></cons> <cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">host</tok> <tok cat="NN">cell</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@16-12-2010
774741703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potent</tok> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activator</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">many</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">viral</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">cellular</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">promoters</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
774741704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">retinoblastoma</tok> <tok cat="NN">susceptibility</tok> <tok cat="NN">gene</tok> <tok cat="NN">product</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Rb</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">dramatically</tok></cons> <cons cat="VP"><tok cat="VBD">suppressed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <cons cat="NP"><tok cat="NN">IE2</tok></cons> <tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NNS">promoters</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
774741705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">unlike</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">another</tok> <cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">suppressor</tok> <tok cat="NN">protein</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p53</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">Rb</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><tok cat="DT">any</tok> <tok cat="JJ">significant</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">basal</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Rb</tok></cons> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="VP"><tok cat="VBZ">interacts</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IE2</tok></cons> <cons cat="CONJP"><tok cat="RB">rather</tok> <tok cat="IN">than</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">general</tok> <tok cat="NN">transcription</tok> <tok cat="NN">machinery</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
774741706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">protein-affinity</tok> <tok cat="NN">chromatography</tok></cons></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Rb</tok></cons> <cons cat="UCP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">translation</tok></cons></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">directly</tok></cons> <cons cat="VP"><tok cat="VBD">bound</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IE2</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
774741707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Rb</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">life</tok> <tok cat="NN">cycle</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HCMV</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">endemic</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">population</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
774741708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">new</tok> <tok cat="NNS">insights</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">slow</tok> <tok cat="NN">rate</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HCMV</tok> <tok cat="NN">DNA</tok> <tok cat="NN">replication</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">possible</tok> <tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HCMV</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">tumorigenesis</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
774744001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok> <tok cat="JJ">replicative</tok> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">de</tok> <tok cat="FW">novo</tok></cons> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">thymocytes</tok></cons></cons></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">simultaneous</tok> <tok cat="JJ">early</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">BZLF-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">repressor</tok></cons> <cons cat="NP"><tok cat="NN">RAZ</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@16-12-2010
774744002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EBV</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">infect</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">epithelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
774744003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="PRP">We</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">others</tok></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">EBV</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VB">infect</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">subset</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">thymocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
774744004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">Infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">thymocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">accompanied</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">appearance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">linear</tok> <tok cat="NN">EBV</tok> <tok cat="NN">genome</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">8</tok> <tok cat="NN">hr</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">infection</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
774744005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">Circularization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">EBV</tok> <tok cat="NN">genome</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">detected</tok><cons cat="NP" ref="i8" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
774744006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="PP" role="PRD"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">contrast</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i9"><tok cat="WRB">where</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">genome</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">circularize</tok> <cons cat="PP" adv="TMP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">24</tok> <tok cat="NN">hr</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">infection</tok></cons></cons></cons></cons><cons cat="ADVP" ref="i9" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
774744007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">appearance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">BamHI</tok> <tok cat="NN">ZLF-1</tok> <tok cat="NN">gene</tok> <tok cat="NN">product</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">ZEBRA</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">RT-PCR</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">8</tok> <tok cat="NN">hr</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">infection</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
774744008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">appearance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <cons cat="NP"><tok cat="NN">fusion</tok> <tok cat="NN">transcript</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RAZ</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i12"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i12" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">comprised</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">regions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">BZLF-1</tok> <tok cat="NN">locus</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">adjacent</tok> <cons cat="NP"><tok cat="NN">BRLF-1</tok> <tok cat="NN">locus</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">RT-PCR</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
774744009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i13" role="SBJ"><tok cat="NN">ZEBRA</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">infected</tok> <cons cat="NP"><tok cat="NNS">thymocytes</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">immunoprecipitation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
774744010@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">EBNA-1</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">infected</tok> <cons cat="NP"><tok cat="NNS">thymocytes</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">transcribed</tok> <cons cat="NP" ref="i14" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Fp</tok> <tok cat="NN">promoter</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">rather</tok> <tok cat="IN">than</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Cp/Wp</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i15"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i15" id="i26" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i26" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="RB">latently</tok> <tok cat="JJ">infected</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
774744011@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i16" role="SBJ"><cons cat="NP"><tok cat="NNS">Transcripts</tok></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gp350/220</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">major</tok> <tok cat="NN">coat</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">EBV</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">identified</tok><cons cat="NP" ref="i16" null="NONE"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">find</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">any</tok> <tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">LMP-2A</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">EBER-1</tok> <tok cat="NNS">loci</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">infected</tok> <cons cat="NP"><tok cat="NNS">thymocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
774744012@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">observations</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">de</tok> <tok cat="FW">novo</tok></cons> <tok cat="NN">EBV</tok> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">thymocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">differs</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
774744013@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">main</tok> <tok cat="NN">difference</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="SBAR" role="PRD"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NNS">thymocytes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i17" role="SBJ"><tok cat="DT">no</tok> <tok cat="NN">evidence</tok> <cons cat="SBAR" ref="i30" null="ICH"></cons></cons><cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" ref="i17" null="NONE"></cons></cons></cons></cons></cons></cons></cons><cons cat="SBAR" id="i30"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">virus</tok></cons> <cons cat="ADVP"><tok cat="RB">ever</tok></cons> <cons cat="VP"><tok cat="VBZ">circularizes</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
774744014@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Rather</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">EBV</tok></cons> <cons cat="VP"><tok cat="VBZ">remains</tok> <cons cat="PP" role="PRD"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">linear</tok> <tok cat="NN">configuration</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="IN">from</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i18" role="SBJ"><tok cat="JJ">replicative</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">transcribed</tok><cons cat="NP" ref="i18" null="NONE"></cons><cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
774744701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">Induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Sp1</tok></cons> <tok cat="NN">phosphorylation</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="JJ">B-independent</tok> <tok cat="NN">HIV</tok> <tok cat="NN">promoter</tok></cons> <tok cat="NN">domain</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">okadaic</tok> <tok cat="NN">acid</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
774744702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">contrast</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">purely</tok> <tok cat="JJ">enhancer-dependent</tok></cons> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cytokines</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">TNF</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">region</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LTR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">okadaic</tok> <tok cat="NN">acid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">OKA</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">enhancer</tok><cons cat="PP" ref="i31" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">responding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">factor</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="PP" id="i31"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">LTR</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@55@@oe@16-12-2010
774744703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">inducibility</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="JJ">LTR-driven</tok> <tok cat="NN">luciferase</tok> <tok cat="NN">expression</tok> <tok cat="NNS">constructs</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">lymphoblastoid</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">OKA</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">depended</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="NN">Sp1</tok> <tok cat="NN">binding</tok> <tok cat="NNS">elements</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TATA</tok> <tok cat="NN">box</tok></cons></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">protein</tok> <cons cat="NP"><tok cat="NN">TBP</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
774744704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="VBN">transformed</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">normal</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">lymphocytes</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">OKA</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">intense</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">constitutively</tok> <tok cat="VBN">expressed</tok></cons> <cons cat="NP"><tok cat="NN">Sp1</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">property</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">OKA</tok></cons></cons> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">shared</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">TNF</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">ester</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">PHA</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="CD">2</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
774744705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">Responsiveness</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">LTR</tok> <tok cat="NNS">constructs</tok></cons> <cons cat="VP"><tok cat="VBN">deleted</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NNS">elements</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">Tat</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">increased</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">OKA</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">TNF</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
774744706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">SP1</tok></cons> <tok cat="NN">phosphorylation</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">OKA</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">selective</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">serine-threonine</tok> <tok cat="NN">phosphatase</tok></cons> <cons cat="NP"><tok cat="NN">PP2A</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">facilitates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">formation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">complex</tok></cons></cons> <cons cat="VP"><tok cat="VBG">involving</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">general</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">Tat</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Sp1</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
774744707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">formation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">complex</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">would</tok> <cons cat="VP"><tok cat="VB">increase</tok><tok cat="COMMA">,</tok> <cons cat="UCP" syn="COOD"><cons cat="ADVP"><cons cat="ADVP"><tok cat="RB">independently</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" ref="i28" null="RNR"></cons></cons></cons><tok cat="DT">an</tok> <cons cat="PP"><tok cat="NN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">synergy</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" ref="i28" null="RNR"></cons></cons></cons></cons><cons cat="NP" id="i28"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="JJ">low</tok> <tok cat="JJ">basal</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">LTR</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
774998501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Human</tok> <cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">transcription</tok></cons> <cons cat="VP"><tok cat="VBN">directed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">carboxyl</tok> <tok cat="NN">terminus</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CIITA</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">defective</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">II</tok> <cons cat="NP"><tok cat="NN">MHC</tok></cons> <tok cat="JJ">combined</tok> <tok cat="JJ">immune</tok> <tok cat="NN">deficiency</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@16-12-2010
774998502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Type</tok> <tok cat="CD">II</tok> <tok cat="JJ">major</tok> <tok cat="NN">histocompatibility</tok> <tok cat="NN">complex</tok></cons> <tok cat="JJ">combined</tok> <tok cat="JJ">immune</tok> <tok cat="NN">deficiency</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">II</tok> <cons cat="NP"><tok cat="NN">MHC</tok></cons> <tok cat="NN">CID</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">bare</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NN">syndrome</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">congenital</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">disease</tok></cons> <cons cat="VP"><tok cat="VBN">characterized</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">absent</tok> <cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@31@@oe@16-12-2010
774998503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="CD">Four</tok> <tok cat="JJ">distinct</tok> <tok cat="NN">complementation</tok> <tok cat="NNS">groups</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">identified</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@16-12-2010
774998504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Recently</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">defective</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">group</tok> <tok cat="CD">II</tok> <tok cat="NN">type</tok> <tok cat="CD">II</tok> <cons cat="NP"><tok cat="NN">MHC</tok></cons> <tok cat="NN">CID</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" ref="i6" null="NONE"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">termed</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NN">CIITA</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
774998505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="PRD"><tok cat="NN">CIITA</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="JJ">gene-specific</tok> <tok cat="NN">transcription</tok> <tok cat="NN">activator</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
774998506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">transcription</tok> <tok cat="NN">activation</tok> <tok cat="NN">function</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">provided</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">N-terminal</tok> <tok cat="JJ">acidic</tok> <tok cat="NN">domain</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">amino</tok> <tok cat="NNS">acids</tok> <tok cat="CD">26-137</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">experimentally</tok> <tok cat="JJ">exchangeable</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">heterologous</tok> <tok cat="JJ">viral</tok> <tok cat="JJ">transcription-activating</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
774998507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">specificity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CIITA</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok> <tok cat="JJ">major</tok> <cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NNS">genes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">DR</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">DQ</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">DP</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="PRPP">its</tok> <tok cat="VBG">remaining</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">C-terminal</tok> <tok cat="NNS">residues</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">amino</tok> <tok cat="NNS">acids</tok> <tok cat="CD">317-1130</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
774998508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">multiple</tok> <tok cat="JJ">cis</tok> <tok cat="NNS">elements</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">especially</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">S</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">X2</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">DR</tok> <tok cat="NN">alpha</tok> <tok cat="JJ">proximal</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">group</tok> <tok cat="CD">II</tok> <tok cat="NN">CID</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><cons cat="NP"><tok cat="NN">CIITA</tok></cons> <tok cat="JJ">dependent</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
774998509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Since</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">CIITA</tok></cons> <tok cat="NN">overexpression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">increase</tok> <cons cat="NP"><tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">propose</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">initiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CIITA</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">serves</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">on-off</tok> <tok cat="NN">switch</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">availability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">downstream</tok> <tok cat="NN">interactor</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">limits</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
775708401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">CAG</tok> <tok cat="NN">repeat</tok> <tok cat="NN">length</tok> <tok cat="NN">variation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">sperm</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">patient</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">Kennedy</tok><tok cat="POS">'s</tok> <tok cat="NN">disease</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@16-12-2010
775708402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="VBN">modified</tok> <cons cat="NP"><tok cat="NN">sperm</tok></cons> <tok cat="NN">typing</tok> <tok cat="NN">protocol</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mutation</tok> <tok cat="NN">frequency</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CAG</tok> <tok cat="NN">repeat</tok> <tok cat="NN">region</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">androgen</tok> <tok cat="NN">receptor</tok> <tok cat="NN">locus</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">measured</tok> <cons cat="NP" ref="i5" null="NONE"></cons></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">rare</tok> <tok cat="NN">semen</tok> <tok cat="NN">sample</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">individual</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">spinal</tok> <tok cat="CC">and</tok> <tok cat="JJ">bulbar</tok> <tok cat="JJ">muscular</tok> <tok cat="NN">atrophy</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SBMA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@41@@oe@16-12-2010
775708403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Among</tok> <cons cat="NP"><tok cat="CD">258</tok> <cons cat="NP"><tok cat="NN">X</tok> <tok cat="JJ">chromosome-containing</tok> <tok cat="NN">sperm</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP"><tok cat="CD">19</tok><tok cat="NN">%</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">repeat</tok> <tok cat="NN">number</tok></cons> <cons cat="ADJP"><tok cat="JJ">equal</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">donor</tok><tok cat="POS">'s</tok> <tok cat="JJ">somatic</tok> <tok cat="NN">DNA</tok></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">47</tok> <tok cat="NNS">repeats</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP"><tok cat="CD">66</tok><tok cat="NN">%</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="NP" role="PRD"><tok cat="NNS">expansions</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP"><tok cat="CD">15</tok><tok cat="NN">%</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="NP" role="PRD"><tok cat="NNS">contractions</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
775708404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">average</tok> <tok cat="NN">expansion</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><tok cat="CD">2.7</tok> <tok cat="NNS">repeats</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@7@@oe@16-12-2010
775708405@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="QP"><tok cat="JJR">More</tok> <tok cat="IN">than</tok> <tok cat="NN">half</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">expansions</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">involved</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">one</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="CD">two</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NNS">repeats</tok></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJS">largest</tok> <cons cat="NP" null="QSTN"></cons></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><tok cat="CD">11</tok> <tok cat="NNS">repeats</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
775708406@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">68</tok><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">contractions</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="NP" syn="COOD" role="PRD"><cons cat="NP"><tok cat="CD">one</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="CD">two</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NNS">repeats</tok></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">six</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">16</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="RB">very</tok> <tok cat="JJ">large</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="QP"><tok cat="CD">12-25</tok></cons> <tok cat="NNS">repeats</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
775708407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="CD">One</tok> <tok cat="NN">contraction</tok></cons> <cons cat="VP"><tok cat="VBD">generated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">allele</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">intermediate</tok> <tok cat="NN">size</tok> <tok cat="NN">range</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="QP"><tok cat="CD">33-39</tok></cons> <tok cat="NNS">repeats</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
775708408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="JJ">Such</tok> <tok cat="NNS">alleles</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">among</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="QP"><tok cat="JJR">more</tok> <tok cat="IN">than</tok> <tok cat="CD">900</tok></cons> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">normal</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">SBMA</tok> <tok cat="NNS">X-chromosomes</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" id="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">examined</tok><cons cat="NP" ref="i10" null="NONE"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
775708409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Comparison</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">SBMA</tok> <cons cat="NP"><tok cat="NN">sperm</tok></cons> <tok cat="NN">typing</tok> <tok cat="NNS">results</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">mutation</tok> <tok cat="NN">frequency</tok> <tok cat="NNS">data</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NNS">alleles</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">supports</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">hypothesis</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">trinucleotide</tok> <tok cat="NN">repeat</tok></cons> <tok cat="NNS">expansions</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">different</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">origin</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><tok cat="NNS">contractions</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
775882001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Isolation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">differentially</tok> <tok cat="VBN">expressed</tok></cons> <tok cat="NN">sequence</tok> <tok cat="NNS">tags</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">steroid-responsive</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">mRNA</tok></cons> <tok cat="JJ">differential</tok> <tok cat="NN">display</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@16-12-2010
775882002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Transcriptional</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">steroid-regulated</tok> <tok cat="NN">gene</tok> <tok cat="NNS">networks</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">nuclear</tok> <tok cat="NN">receptor</tok> <tok cat="NNS">proteins</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">coordinate</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">limited</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">target</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@16-12-2010
775882003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="JJ">much</tok> <cons cat="PP" ref="i30" null="ICH"></cons></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="NP" ref="i5" null="NONE"></cons></cons></cons><cons cat="PP" id="i30"><tok cat="IN">about</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">structure</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">function</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">steroid</tok> <tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="ADJP"><tok cat="RB">relatively</tok> <tok cat="JJ">few</tok></cons> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">cell-specific</tok></cons> <cons cat="ADJP"><tok cat="JJ">steroid-regulated</tok></cons></cons> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">isolated</tok></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">characterized</tok></cons></cons><cons cat="NP" ref="i6" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
775882004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">paper</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">describe</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">mRNA</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">differential</tok> <tok cat="NN">display</tok> <cons cat="NP"><tok cat="JJ">reverse</tok> <tok cat="NN">transcriptase</tok></cons> <tok cat="NN">PCR</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">DDPCR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">identify</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">isolate</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><cons cat="NP"><tok cat="JJ">short</tok> <tok cat="NN">cDNA</tok> <tok cat="NN">sequence</tok> <tok cat="NNS">tags</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">thymocyte</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">prostate</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NN">hormone</tok> <tok cat="NNS">conditions</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
775882005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">technique</tok></cons></cons></cons> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP"><tok cat="JJ">several</tok> <cons cat="NP"><cons cat="NP"><tok cat="RB">differentially</tok> <tok cat="VBN">expressed</tok> <tok cat="NN">sequence</tok> <tok cat="NNS">tags</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">DESTs</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">mouse</tok> <tok cat="NN">thymocyte</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="NP"><tok cat="NN">WEHI</tok> <tok cat="CD">7.2</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
775882006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="CD">Two</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NNS">DESTs</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">GIG10</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">GIG18</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">dexamethasone</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">2</tok> <tok cat="NN">h</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">treatment</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
775882007@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">GIG10</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="NN">sequence</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">GIG18</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mouse</tok> <tok cat="NN">homologue</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="VBN">expressed</tok> <tok cat="NN">sequence</tok> <tok cat="NN">tag</tok></cons></cons> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">B</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
775882008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">used</tok> <cons cat="NP"><tok cat="NN">DDPCR</tok></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">isolate</tok> <cons cat="NP"><tok cat="NNS">DESTs</tok> <cons cat="SBAR" ref="i33" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">androgen-modulated</tok> <tok cat="NN">rat</tok> <tok cat="JJ">ventral</tok> <tok cat="NN">prostate</tok> <tok cat="NN">tissue</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" id="i33"><cons cat="WHNP" id="i11"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="WHPP"><tok cat="IN">of</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">characterized</tok> <cons cat="NP" ref="i11" null="T"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">correspond</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">3'</tok> <tok cat="NN">end</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">prostatic</tok> <tok cat="NN">spermine</tok> <tok cat="NN">binding</tok> <tok cat="NN">protein</tok> <tok cat="NN">mRNA</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">known</tok> <cons cat="NP"><tok cat="JJ">androgen-regulated</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
775882009@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i13" role="SBJ"><cons cat="NP"><tok cat="NNS">Modifications</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">original</tok> <tok cat="NN">DDPCR</tok> <tok cat="NN">protocol</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i12"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="S"><cons cat="COMP" null="ZERO"></cons><cons cat="S"><cons cat="NP" ref="i12" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">potentially</tok></cons> <cons cat="VP"><tok cat="VB">decrease</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">frequency</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBG">isolating</tok> <tok cat="JJ">false-positive</tok> <cons cat="NP"><tok cat="NNS">DESTs</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">described</tok> <cons cat="NP" ref="i13" null="NONE"></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">merits</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DDPCR</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="ADJP"><tok cat="JJ">relative</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="JJ">differential</tok> <tok cat="NN">cDNA</tok> <tok cat="NN">cloning</tok> <tok cat="NNS">strategies</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">discussed</tok><cons cat="NP" ref="i14" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
775954801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Identification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">novel</tok> <cons cat="NP"><tok cat="JJ">regulatory</tok> <tok cat="NNS">elements</tok></cons></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">5'-untranslated</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">A</tok> <tok cat="NN">gamma-globin</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@16-12-2010
775954802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Interaction</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stage</tok> <tok cat="NN">selector</tok> <tok cat="NN">element</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SSE</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">proximal</tok> <tok cat="NN">gamma-globin</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">hypersensitivity</tok> <tok cat="NN">site</tok> <tok cat="CD">2</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">locus</tok> <tok cat="NN">control</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">partly</tok></cons> <cons cat="VP"><tok cat="VBZ">mediates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">competitive</tok> <tok cat="NN">silencing</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">beta-globin</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">fetal</tok> <tok cat="JJ">developmental</tok> <tok cat="NN">stage</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@38@@oe@16-12-2010
775954803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">second</tok> <cons cat="NP"><tok cat="JJ">SSE-like</tok> <tok cat="NN">element</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">5'-untranslated</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">gamma-gene</tok></cons></cons></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">contributes</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">competitive</tok> <tok cat="NN">silencing</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">beta-gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
775954804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Utilizing</tok> <cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">transfection</tok> <tok cat="NNS">assays</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">fetal</tok> <tok cat="JJ">erythroid</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">K562</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">core</tok> <tok cat="NN">enhancer</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">hypersensitivity</tok> <tok cat="NN">site</tok> <tok cat="CD">2</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">preferentially</tok></cons> <cons cat="VP"><tok cat="VB">interact</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">proximal</tok> <tok cat="NN">gamma-promoter</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">absence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">SSE</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">completely</tok></cons> <cons cat="VP"><tok cat="VBG">silencing</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="VBN">linked</tok> <tok cat="NN">beta-promoter</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
775954805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Mutation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">20-base</tok> <tok cat="NN">pair</tok> <tok cat="NN">sequence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gamma-gene</tok> <tok cat="JJ">5'-untranslated</tok> <tok cat="NN">region</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">UTR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">led</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">derepression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">beta-promoter</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
775954806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">marked</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gamma-promoter</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">mutation</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">repressor</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
775954807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Fine</tok> <tok cat="NN">mutagenesis</tok></cons> <cons cat="VP"><tok cat="VBD">dissected</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">activities</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">regions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">5'-UTR</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
775954808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">stage</tok> <tok cat="NN">selector</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">localized</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">region</tok></cons> <cons cat="ADJP"><tok cat="JJ">centered</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NNS">nucleotides</tok> <tok cat="CD">+13</tok> <tok cat="TO">to</tok> <tok cat="CD">+15</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
775954809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Electromobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons> <cons cat="VP"><tok cat="VBG">utilizing</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">sequence</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="DT">a</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">fetal</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">erythroid-specific</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">protein</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
775954810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">repressor</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">5'-UTR</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">localized</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">tandem</tok> <cons cat="NP"><tok cat="JJ">GATA-like</tok> <tok cat="NNS">sites</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">appear</tok> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">complex</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">proteins</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i11"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="WHPP"><tok cat="IN">of</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons></cons> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="NP"><tok cat="NN">GATA-1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
775954811@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">5'-UTR</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">gamma-gene</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">sequences</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i13"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i13" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><tok cat="PRPP">its</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">transcriptional</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">developmental</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NN">regulation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
775987501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Abnormal</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">lpr</tok> <cons cat="NP"><tok cat="JJ">CD4-CD8-</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">constitutive</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">novel</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="IN">of</tok> <tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="JJ">cells-binding</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@26@@oe@16-12-2010
775987502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">inert</tok> <tok cat="NN">quality</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MRL-Ipr/Ipr</tok> <tok cat="LRB">(</tok><tok cat="NN">Ipr</tok><tok cat="RRB">)</tok> <tok cat="JJ">peripheral</tok> <tok cat="JJ">CD4-CD8-</tok> <tok cat="LRB">(</tok><tok cat="JJ">CD4-8-</tok><tok cat="RRB">)</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">manifests</tok> <cons cat="ADVP"><tok cat="RB">primarily</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">inability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">proliferate</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="VP"><tok cat="VB">produce</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">IL-2</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">TCR</tok> <cons cat="NP" ref="i26" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">mitogenic</tok> <cons cat="NP" ref="i26" null="RNR"></cons></cons><cons cat="NP" id="i26"><tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@33@@oe@16-12-2010
775987503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Yet</tok></cons> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="JJ">same</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">do</tok> <cons cat="VP"><tok cat="VB">initiate</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">early</tok> <tok cat="JJ">TCR-mediated</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">events</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">generation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">inositol</tok> <tok cat="NNS">phosphates</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="JJ">intracellular</tok> <tok cat="NN">calcium</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
775987504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">They</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">display</tok> <cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">p59fyn</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD3</tok> <tok cat="NN">zeta</tok></cons> <tok cat="NN">tyrosine</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NN">phosphorylation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
775987505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">generation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">second</tok> <tok cat="NNS">messengers</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">normally</tok></cons> <cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">downstream</tok> <tok cat="NN">signaling</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
775987506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok><tok cat="COMMA">,</tok></cons> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">compared</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok> <tok cat="NN">state</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">gene</tok> <tok cat="NN">promoter</tok> <tok cat="NN">region</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">freshly</tok> <tok cat="VBN">isolated</tok></cons> <cons cat="NP" ref="i24" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="VBN">stimulated</tok> <cons cat="NP" ref="i24" null="RNR"></cons></cons><cons cat="NP" id="i24"><tok cat="NN">Ipr</tok> <tok cat="JJ">CD4-8-</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
775987507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NNS">Levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">octamer</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">p50-p65</tok> <tok cat="NN">heterodimer</tok></cons><tok cat="RRB">)</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <cons cat="VP"><tok cat="VBN">elevated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">freshly</tok> <tok cat="VBN">isolated</tok></cons> <cons cat="NP"><tok cat="NN">Ipr</tok> <tok cat="JJ">CD4-8-</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">activated</tok> <tok cat="NN">phenotype</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
775987508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NNS">mitogens</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">formation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBG">transactivating</tok> <tok cat="NN">complex</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="IN">of</tok> <tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-AT</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NNS">kinetics</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Ipr</tok> <tok cat="JJ">CD4-8-</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
775987509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Yet</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBG">activating</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">never</tok></cons> <cons cat="VP"><tok cat="VBP">reach</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">similarly</tok> <tok cat="VBN">stimulated</tok></cons> <tok cat="JJ">normal</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
775987510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">Ipr</tok> <tok cat="JJ">CD4-8-</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">display</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">novel</tok></cons> <cons cat="ADJP"><tok cat="JJ">specific</tok></cons></cons> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok> <tok cat="NN">site</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">much</tok> <tok cat="JJR">lower</tok></cons> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">freshly</tok> <tok cat="VBN">isolated</tok></cons> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
775987511@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Upon</tok> <cons cat="NP"><tok cat="JJ">mitogenic</tok> <tok cat="NN">stimulation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">novel</tok> <cons cat="NP"><tok cat="JJ">NF-AT-binding</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok></cons> <cons cat="VP"><tok cat="VBN">down-regulated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBZ">persists</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">levels</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Ipr</tok> <tok cat="JJ">CD4-8-</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
775987512@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">These</tok> <tok cat="CD">two</tok> <tok cat="NNS">abnormalities</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok> <tok cat="NN">site</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potential</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i11" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">account</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">defect</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">Ipr</tok> <tok cat="JJ">CD4-8-</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i11" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
775995601@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">GM-CSF</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons> <cons cat="VP"><tok cat="VBP">share</tok> <cons cat="NP"><tok cat="JJ">common</tok> <tok cat="NN">control</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">costimulatory</tok> <tok cat="NNS">signals</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@16-12-2010
775995602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Antigen</tok></cons> <cons cat="VP"><tok cat="VBN">complexed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">major</tok> <tok cat="NN">histocompatibility</tok> <tok cat="NN">complex</tok> <tok cat="NN">class</tok> <cons cat="NP"><tok cat="CD">I</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="CD">II</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">molecules</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">surface</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">antigen</tok> <tok cat="NN">presenting</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">interacts</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TCR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">surface</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">initiates</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">activation</tok> <tok cat="NN">cascade</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@39@@oe@16-12-2010
775995603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">So</tok> <tok cat="VBN">called</tok></cons> <tok cat="JJ">costimulatory</tok> <tok cat="NNS">signals</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <tok cat="NNS">interactions</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">soluble</tok> <tok cat="NNS">cytokines</tok></cons> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">antigen</tok> <tok cat="NN">presenting</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">complete</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
775995604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">High</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok></cons> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">required</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">TCR</tok> <cons cat="NP" ref="i26" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">costimulatory</tok> <cons cat="NP" ref="i26" null="RNR"></cons></cons><cons cat="NP" id="i26"><tok cat="NNS">signals</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
775995605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">granulocyte-macrophage</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NNS">sequences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">powerful</tok> <cons cat="NP"><tok cat="NN">enhancer</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="ADVP"><cons cat="NP"><tok cat="RB">3kb</tok></cons> <tok cat="RB">upstream</tok></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">respond</tok> <cons cat="PP"><tok cat="TO">to</tok> <tok cat="JJ">TCR-like</tok> <tok cat="NNS">signals</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
775995606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">These</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="CC">and</tok> <tok cat="NN">enhancer</tok> <tok cat="NNS">regions</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">mainly</tok></cons> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="IN">of</tok> <tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NFAT</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
775995607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFAT</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">TCR</tok></cons> <tok cat="NNS">signals</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="ADVP"><tok cat="RB">well</tok></cons> <cons cat="VP"><tok cat="VBN">described</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-2</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-4</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
775995608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Costimulatory</tok> <tok cat="NNS">signals</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CD28</tok> <tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <tok cat="NN">molecule</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">lead</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">activation</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">distinct</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">granulocyte-macrophage</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok> <tok cat="LRB">(</tok><tok cat="NN">GM-CSF</tok><tok cat="RRB">)</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
775995609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">region</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">termed</tok> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="NP" syn="COOD" role="PRD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CK-1</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">CD28RE</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">family</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
775995610@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="JJ">Human</tok> <tok cat="NN">T</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HTLV-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">infects</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">lead</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">increase</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GM-CSF</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
775995611@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HTLV-1</tok> <tok cat="NN">transactivator</tok> <tok cat="NN">protein</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">tax</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">acts</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">costimulatory</tok> <tok cat="NN">signal</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">GM-CSF</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">cooperate</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">TCR</tok></cons> <tok cat="NNS">signals</tok></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">mediate</tok> <cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NN">level</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
775995612@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Tax</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GM-CSF</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CK-1/CD28RE</tok> <tok cat="NN">region</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
775995613@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i13" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="JJ">other</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NNS">coactivators</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tax</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" id="i14" role="SBJ"><tok cat="DT">these</tok></cons> <cons cat="VP"><tok cat="VBP">remain</tok> <cons cat="S"><cons cat="NP" ref="i14" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">identified</tok><cons cat="NP" ref="i14" null="NONE"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
775995614@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">CK-1/CD28RE</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GM-CSF</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">shows</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">high</tok> <tok cat="NN">degree</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">similarity</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons> <cons cat="NP"><tok cat="NN">CD28RE</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-3</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">related</tok> <tok cat="NN">region</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
775995615@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">observation</tok><tok cat="COMMA">,</tok></cons> <cons cat="ADVP"><tok cat="RB">together</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">fact</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">GM-CSF</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons> <cons cat="VP"><tok cat="VBP">respond</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">TCR</tok></cons> <tok cat="NNS">signals</tok></cons></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="NN">NFAT</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">implies</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">high</tok> <tok cat="NN">degree</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">conservation</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok></cons> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
776080701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">MIP1</tok> <tok cat="NN">alpha</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">protein</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MNP</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">crucial</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">MIP-1</tok> <tok cat="NN">alpha</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@28@@oe@16-12-2010
776080702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Murine</tok> <tok cat="NN">macrophage</tok> <tok cat="JJ">inflammatory</tok> <tok cat="NN">protein</tok> <tok cat="CD">1</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MIP-1</tok> <tok cat="NN">alpha</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">equivalent</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">GOS19</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">LD78</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">AT464</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">-C-C</tok> <tok cat="NN">family</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">low-molecular-weight</tok> <tok cat="NNS">chemokines</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@32@@oe@16-12-2010
776080703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">Secreted</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">bone</tok> <cons cat="NP"><tok cat="JJ">marrow-derived</tok> <tok cat="NN">MIP-1</tok> <tok cat="NN">alpha/GOS19</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><tok cat="JJ">primitive</tok> <tok cat="JJ">hematopoietic</tok> <tok cat="NN">stem</tok> <tok cat="NNS">cells</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">homeostatic</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stem</tok> <tok cat="NN">cell</tok></cons> <tok cat="NN">proliferation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
776080704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">chemotaxis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NNS">responses</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
776080705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRP">it</tok></cons> <cons cat="S" ref="i6" null="EXP"></cons></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok></cons> <cons cat="S" id="i6"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">understand</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">control</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MIP-1</tok> <tok cat="NN">alpha/GOS19</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
776080706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Previous</tok> <tok cat="NN">work</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">set</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">widely</tok> <tok cat="VBN">expressed</tok></cons> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">ICK-1</tok> <tok cat="NN">family</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBP">affect</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GOS19</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
776080707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">One</tok> <tok cat="NN">member</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">ICK-1A</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">behaves</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">strong</tok> <tok cat="JJ">negative</tok> <tok cat="NN">regulator</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
776080708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">communication</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><tok cat="NN">evidence</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathway</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">induction</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">macrophage</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="NP"><tok cat="NN">U937</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">different</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
776080709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">ICK-1</tok> <tok cat="NN">binding</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">confer</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">negative</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
776080710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">additional</tok> <cons cat="NP"><tok cat="VBG">binding</tok> <tok cat="NN">site</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">MIP-1</tok> <tok cat="NN">alpha</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">protein</tok> <tok cat="LRB">(</tok><tok cat="NN">MNP</tok><tok cat="RRB">)</tok> <tok cat="NN">site</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">overlaps</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">ICK-1</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
776080711@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Interaction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">tissue</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">MNP</tok> <tok cat="NN">site</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">formation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">fast-migrating</tok> <tok cat="JJ">protein-DNA</tok> <tok cat="NNS">complexes</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">similar</tok></cons> <tok cat="CC">but</tok> <cons cat="ADJP"><tok cat="JJ">distinct</tok></cons></cons> <tok cat="JJ">electrophoretic</tok> <tok cat="NNS">mobilities</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
776080712@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">mutation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">MNP</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">abrogate</tok> <cons cat="NP"><tok cat="NN">ICK-1</tok> <tok cat="NN">binding</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">inactivates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GOS19.1</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">reduces</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="RB">fourfold</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
776080713@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">propose</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">MNP</tok> <tok cat="NN">protein</tok></cons><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons> <cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">MNP</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">constitutes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
776081001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Ligand-dependent</tok> <tok cat="NN">repression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="NP"><tok cat="NN">GATA-1</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">estrogen</tok> <tok cat="NN">receptor</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
776081002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">High-dose</tok> <tok cat="NN">estrogen</tok> <tok cat="NN">administration</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><tok cat="NN">anemia</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">mammals</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@8@@oe@16-12-2010
776081003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NNS">chickens</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NNS">estrogens</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">stimulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">outgrowth</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">bone</tok> <tok cat="JJ">marrow-derived</tok> <tok cat="JJ">erythroid</tok> <tok cat="NN">progenitor</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">delay</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">maturation</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
776081004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">delay</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">down-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">many</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="JJ">cell-specific</tok> <tok cat="NNS">genes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">alpha-</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">beta-globin</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">band</tok> <tok cat="CD">3</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">band</tok> <tok cat="CD">4.1</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="JJ">cell-specific</tok> <tok cat="NN">histone</tok></cons> <cons cat="NP"><tok cat="NN">H5</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
776081005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">estrogens</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">reduce</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NN">progenitor</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">human</tok> <tok cat="NN">bone</tok> <tok cat="NN">marrow</tok> <tok cat="NNS">cultures</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
776081006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">address</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">potential</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">estrogens</tok></cons> <cons cat="VP"><tok cat="VBP">suppress</tok> <cons cat="NP"><tok cat="NN">erythropoiesis</tok></cons><cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GATA-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">participates</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">majority</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="JJ">cell-specific</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">necessary</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">full</tok> <tok cat="NN">maturation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">erythrocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
776081007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBN">repressed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">estrogen</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ER</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">ligand-dependent</tok> <tok cat="NN">manner</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">repression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">reversible</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">4-hydroxytamoxifen</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
776081008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">ER-mediated</tok> <tok cat="NN">repression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GATA-1</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">artificial</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">single</tok> <cons cat="NP"><tok cat="JJ">GATA-binding</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">context</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">intact</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" id="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">normally</tok></cons> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">GATA-1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
776081009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">GATA-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">ER</tok></cons></cons> <cons cat="VP"><tok cat="VBP">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">each</tok> <tok cat="JJ">other</tok></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">absence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DNA</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
776081010@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">coimmunoprecipitation</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="VBN">transfected</tok> <cons cat="NP"><tok cat="NN">COS</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">GATA-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">ER</tok></cons></cons> <cons cat="VP"><tok cat="VBP">associate</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">ligand-dependent</tok> <tok cat="NN">manner</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
776081011@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Mapping</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">GATA-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">ER</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">form</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok> <tok cat="CD">two</tok></cons> <tok cat="NNS">contacts</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i11"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">involve</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">finger</tok> <tok cat="NN">region</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">N-terminal</tok> <tok cat="NN">activation</tok> <tok cat="NN">domain</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
776081012@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">speculate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="NNS">estrogens</tok></cons> <cons cat="VP"><tok cat="VBP">exert</tok> <cons cat="NP"><tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">erythropoiesis</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">modulating</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GATA-1</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">protein-protein</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">ER</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
776081013@GENIA Treebank@formal@@1@S@<cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">ABSTRACT</tok></cons> <cons cat="VP"><tok cat="VBN">TRUNCATED</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">AT</tok> <cons cat="NP"><tok cat="CD">250</tok> <tok cat="NNS">WORDS</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons>@@@@1@7@@oe@16-12-2010
776081401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">A</tok> <cons cat="NP"><tok cat="JJ">Myc-associated</tok> <tok cat="NN">zinc</tok> <tok cat="NN">finger</tok> <tok cat="NN">protein</tok> <tok cat="NN">binding</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">four</tok> <tok cat="JJ">important</tok> <cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="NNS">regions</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CD4</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
776081402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">CD4</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">developmental</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD4</tok></cons> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
776081403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">report</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">minimal</tok> <cons cat="NP"><tok cat="NN">CD4</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">four</tok> <cons cat="NP"><tok cat="NN">factor</tok> <tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i5"><cons cat="NP"><tok cat="DT">each</tok></cons> <cons cat="WHPP"><tok cat="IN">of</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons></cons> <cons cat="S"><cons cat="NP" ref="i5" id="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">full</tok> <tok cat="NN">function</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
776081404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">biochemical</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">mutagenesis</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">analyses</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">determined</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">multiple</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="VP"><tok cat="VBP">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="JJ">independent</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
776081405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">determined</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i9" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">initiator-like</tok> <tok cat="NN">sequence</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">cap</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">Ets</tok> <tok cat="NN">consensus</tok> <tok cat="NN">sequence</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">full</tok> <cons cat="NP"><tok cat="NN">promoter</tok></cons> <tok cat="NN">function</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
776081406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Myc-associated</tok> <tok cat="NN">zinc</tok> <tok cat="NN">finger</tok> <tok cat="NN">protein</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MAZ</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">predominant</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
776081407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="JJ">last</tok> <tok cat="NN">site</tok></cons> <cons cat="ADVP"><tok cat="RB">closely</tok></cons> <cons cat="VP"><tok cat="VBZ">resembles</tok> <cons cat="NP"><cons cat="NP" id="i11"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">ME1a1</tok> <tok cat="NN">G3AG4AG3</tok> <tok cat="NN">motif</tok></cons></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">critical</tok> <tok cat="NN">element</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">P2</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">c-myc</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
776081408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons> <cons cat="VP"><tok cat="VBP">believe</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">MAZ</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">likely</tok> <cons cat="S"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">developmental</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CD4</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
776894101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Nm23H2</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" ref="i30" null="RNR"></cons></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP" ref="i30" null="RNR"></cons></cons><cons cat="NP" id="i30"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">translocated</tok> <cons cat="NP"><tok cat="NN">c-myc</tok> <tok cat="NN">allele</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Burkitt</tok><tok cat="POS">'s</tok> <tok cat="NN">lymphoma</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
776894102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <tok cat="NN">footprint</tok></cons> <cons cat="PP"><tok cat="IN">over</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">PuF</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">translocated</tok> <cons cat="NP"><tok cat="NN">c-myc</tok> <tok cat="NN">allele</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Burkitt</tok><tok cat="POS">'s</tok> <tok cat="NN">lymphoma</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@22@@oe@16-12-2010
776894103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">PuF</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">silent</tok> <tok cat="JJ">normal</tok> <tok cat="NN">c-myc</tok> <tok cat="NN">allele</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unoccupied</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
776894104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">antibody</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">UV</tok> <tok cat="NN">cross-linking</tok></cons> <cons cat="VP"><tok cat="VBN">followed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">SDS-gel</tok> <tok cat="NN">electrophoresis</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Western</tok> <tok cat="NN">analysis</tok></cons></cons></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Nm23H2</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">bound</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">c-myc</tok> <cons cat="NP"><tok cat="NN">PuF</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
776894105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Transfection</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-myc</tok> <tok cat="NN">promoter</tok> <tok cat="NNS">constructs</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">DHL-9</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Raji</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">PuF</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="VP"><tok cat="VBD">functioned</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">positive</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">element</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">drop</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">mutation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
776894106@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">Access</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">site</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="JJ">silent</tok> <tok cat="NN">c-myc</tok> <tok cat="NN">allele</tok></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Nm23H2</tok></cons> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">deregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">translocated</tok> <cons cat="NP"><tok cat="NN">c-myc</tok> <tok cat="NN">allele</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Burkitt</tok><tok cat="POS">'s</tok> <tok cat="NN">lymphoma</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
776983401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">pp90rsk</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">growth</tok> <tok cat="NN">response-1</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">pokeweed</tok> <tok cat="NN">mitogen</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@16-12-2010
776983402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">present</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">pokeweed</tok> <tok cat="NN">mitogen</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PWM</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">growth</tok> <tok cat="NN">response-1</tok> <tok cat="NN">gene</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EGR-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@30@@oe@16-12-2010
776983403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PWM</tok></cons> <cons cat="VP"><tok cat="VBZ">regulates</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">EGR-1</tok></cons> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">transcriptional</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">post-transcriptional</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">mechanisms</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
776983404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Transient</tok> <tok cat="NN">transfection</tok> <tok cat="NNS">assays</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">EGR-1</tok> <tok cat="NN">promoter</tok> <tok cat="NNS">fragments</tok></cons> <cons cat="VP"><tok cat="VBN">linked</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">chloramphenicol</tok> <tok cat="NN">acetyltransferase</tok> <tok cat="LRB">(</tok><tok cat="NN">CAT</tok><tok cat="RRB">)</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">PWM</tok></cons> <tok cat="JJ">induced</tok> <cons cat="NP"><tok cat="NN">EGR-1</tok></cons> <tok cat="NN">transcription</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">conferred</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CArG</tok> <tok cat="NN">motif</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">C</tok> <tok cat="NN">C[AT]6GG</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">EGR-1</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
776983405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="ADVP"><tok cat="RB">further</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">S6</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">pp90rsk</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">evidenced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">S6</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">serum</tok> <tok cat="NN">response</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SRF</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">peptides</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">PWM</tok> <tok cat="JJ">treated</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
776983406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">Taken</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">together</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="NN">PWM</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">initiate</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">intracytoplasmic</tok> <tok cat="NN">signalling</tok> <tok cat="NN">cascade</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">EGR-1</tok></cons> <tok cat="NN">induction</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
777008501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Infection</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="FW">Theileria</tok> <tok cat="FW">annulata</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">matrix</tok> <tok cat="NN">metalloproteinase</tok> <tok cat="CD">9</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">bovine</tok> <tok cat="NNS">leucocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@16-12-2010
777008502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="FW">Theileria</tok> <tok cat="FW">annulata</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">infects</tok> <cons cat="NP"><tok cat="JJ">bovine</tok> <tok cat="NNS">leucocytes</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">reversible</tok> <tok cat="NN">transformation</tok></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">such</tok> <tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBP">become</tok> <cons cat="ADJP" syn="COOD" role="PRD"><cons cat="ADJP"><tok cat="VBN">immortalised</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">metastatic</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
777008503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBZ">describes</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">parasite-induced</tok> <tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">host</tok> <tok cat="NN">cell</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">direct</tok> <tok cat="NN">bearing</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">transformation</tok> <tok cat="NN">process</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
777008504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="FW">T.</tok> <tok cat="JJ">annulata-infected</tok> <tok cat="NNS">leucocytes</tok></cons> <cons cat="VP"><tok cat="VBP">produce</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">novel</tok> <cons cat="NP"><tok cat="NN">metalloproteinase</tok></cons> <tok cat="NNS">activities</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
777008505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" id="i9"><cons cat="NP"><tok cat="CD">One</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">called</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NN">B1</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">97-kDa</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" id="i12" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">secreted</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">large</tok> <tok cat="NNS">amounts</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">purified</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">protein-free</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">conditioned</tok></cons></cons> <tok cat="NN">medium</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
777008506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i13" role="SBJ"><cons cat="NP"><tok cat="DT">An</tok> <tok cat="NN">antiserum</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">enzyme</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i13" null="NONE"></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">isolate</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">cDNA</tok> <tok cat="NN">clone</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
777008507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="VBN">predicted</tok> <tok cat="NN">protein</tok> <tok cat="NN">sequence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">B1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><cons cat="NP"><tok cat="CD">81</tok><tok cat="NN">%</tok></cons> <tok cat="JJ">identical</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">matrix</tok> <tok cat="NN">metalloproteinase</tok> <tok cat="CD">9</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MMP9</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">demonstrating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">bovine</tok> <tok cat="NN">homologue</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">enzyme</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
777008508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">RNAase</tok></cons> <tok cat="NN">protection</tok> <tok cat="NNS">assays</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">MMP9</tok></cons> <tok cat="NN">activity</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">unique</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">infected</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">due</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="VBN">increased</tok> <cons cat="NP"><tok cat="NN">MMP9</tok></cons> <tok cat="NN">mRNA</tok> <tok cat="NNS">levels</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
777008509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">assayed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">constitutively</tok> <tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NNS">amounts</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">Theileria-infected</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
777008510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">assayed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">mRNA</tok></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-Fos</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">common</tok> <tok cat="NN">component</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i15" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">indeed</tok></cons> <cons cat="VP"><tok cat="VBN">up-regulated</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">infected</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
777008511@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Since</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i16" role="SBJ"><tok cat="NN">AP-1</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP" ref="i16" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">MMP9</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">confer</tok> <cons cat="NP"><tok cat="JJ">metastatic</tok> <tok cat="NNS">properties</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="PP" role="PRD"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">considerable</tok> <tok cat="NN">significance</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">respect</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">transformed</tok> <tok cat="NN">phenotype</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="FW">Theileria</tok> <tok cat="NN">infection</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
777972601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="VBN">Reduced</tok> <tok cat="JJ">mitogenic</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">prognostic</tok> <tok cat="NN">parameter</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">course</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">breast</tok> <tok cat="NN">cancer</tok></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">prospective</tok></cons> <cons cat="ADJP"><tok cat="JJ">longitudinal</tok></cons></cons> <tok cat="NN">study</tok></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@16-12-2010
777972602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">Immunosuppression</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="ADVP"><tok cat="RB">often</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">course</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">malignant</tok> <tok cat="NNS">diseases</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@12@@oe@16-12-2010
777972603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i16" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">proliferation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">PBMCs</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">mitogenic</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phytohaemagglutinin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PHA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">assessed</tok> <cons cat="NP" ref="i16" null="NONE"></cons><cons cat="ADVP" adv="TMP"><tok cat="RB">prospectively</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">90</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">stage</tok> <tok cat="CD">I-III</tok> <tok cat="NN">breast</tok> <tok cat="NN">cancer</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
777972604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Whereas</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="JJ">PHA-induced</tok> <tok cat="NN">proliferation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">PBMCs</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">breast</tok> <tok cat="NN">cancer</tok></cons></cons></cons></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">preoperatively</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP"><tok cat="VBN">decreased</tok> <cons cat="NP" ref="i6" null="NONE"></cons></cons><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i7"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="JJ">control</tok> <tok cat="NNS">individuals</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">P</tok></cons> <cons cat="ADJP"><tok cat="JJR">&lt;</tok> <cons cat="NP"><tok cat="CD">0.001</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i7" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">degree</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">defect</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">PHA-induced</tok> <tok cat="NN">proliferation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">PBMCs</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">depended</tok> <cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">tumour</tok> <tok cat="NN">burden</tok></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">manifested</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">tumour</tok> <tok cat="NN">size</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">axillary</tok> <tok cat="NN">lymph</tok> <tok cat="NN">node</tok> <tok cat="NN">involvement</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">P</tok></cons> <cons cat="ADJP"><tok cat="JJR">&lt;</tok> <cons cat="NP"><tok cat="CD">0.003</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">each</tok> <tok cat="NN">case</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@64@@oe@16-12-2010
777972605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">PHA-induced</tok> <tok cat="NN">proliferation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">PBMCs</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">dropped</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WP">who</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">received</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">adjuvant</tok> <tok cat="NN">chemotherapy</tok></cons> <cons cat="VP"><tok cat="VBG">consisting</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">cyclophosphamide</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">methotrexate</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">fluorouracil</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CMF</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">observation</tok> <tok cat="NN">period</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">6</tok> <tok cat="NNS">months</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">P</tok></cons> <cons cat="ADJP"><tok cat="JJR">&lt;</tok> <cons cat="NP"><tok cat="CD">0.01</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">adjuvant</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tamoxifen</tok></cons> <cons cat="ADVP"><tok cat="RB">only</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@16-12-2010
777972606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><cons cat="PP"><tok cat="IN">After</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">additional</tok> <tok cat="CD">6</tok> <tok cat="NNS">months</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="PP"><tok cat="FW">i.e.</tok></cons> <cons cat="PP" adv="TMP"><cons cat="NP"><tok cat="CD">12</tok> <tok cat="NNS">months</tok></cons> <tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">surgery</tok></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">PHA-induced</tok> <tok cat="NN">proliferation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">PBMCs</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">similar</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">adjuvant</tok> <tok cat="NN">chemotherapy</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">CMF</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="VP"><tok cat="VBG">receiving</tok> <cons cat="NP"><tok cat="JJ">continued</tok> <tok cat="JJ">adjuvant</tok> <tok cat="NN">tamoxifen</tok> <tok cat="NN">treatment</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">P</tok></cons> <cons cat="ADJP"><tok cat="JJR">&gt;</tok> <cons cat="NP"><tok cat="CD">0.1</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">all</tok> <tok cat="NNS">patients</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">still</tok> <tok cat="RB">significantly</tok></cons> <cons cat="VP"><tok cat="VBD">decreased</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">controls</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">P</tok></cons> <cons cat="ADJP"><tok cat="JJR">&lt;</tok> <cons cat="NP"><tok cat="CD">0.001</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@58@@oe@16-12-2010
777972607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i10"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" null="NONE"></cons><cons cat="UCP" syn="COOD" adv="TMP"><cons cat="ADVP" adv="TMP"><tok cat="RB">preoperatively</tok></cons> <tok cat="CC">and</tok> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="CD">12</tok> <tok cat="NNS">months</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">compared</tok><cons cat="NP" ref="i11" null="NONE"></cons><cons cat="ADVP" ref="i10" null="T"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="RB">out</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">23</tok> <tok cat="NNS">patients</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i12"><tok cat="WPP">whose</tok> <cons cat="NP"><tok cat="NNS">PBMCs</tok></cons></cons> <cons cat="S"><cons cat="NP" ref="i12" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">had</tok> <cons cat="VP"><tok cat="VBN">experienced</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">drop</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">PHA-induced</tok> <tok cat="NN">proliferation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">14</tok> <cons cat="NP" null="QSTN"></cons></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">61</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="VP"><tok cat="VBN">developed</tok> <cons cat="NP"><tok cat="JJ">metastatic</tok> <tok cat="NN">disease</tok></cons> <cons cat="PP"><cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">subsequent</tok> <tok cat="CD">24</tok> <tok cat="NNS">months</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="PP"><tok cat="FW">i.e.</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="CD">36</tok> <tok cat="NNS">months</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">surgery</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@52@@oe@16-12-2010
777972608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="RB">out</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">59</tok> <tok cat="NNS">patients</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i13"><tok cat="WPP">whose</tok> <cons cat="NP"><tok cat="NNS">PBMCs</tok></cons></cons> <cons cat="S"><cons cat="NP" ref="i13" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">PHA-induced</tok> <tok cat="NN">proliferation</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="CD">12</tok> <tok cat="NNS">months</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">surgery</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="QP"><tok cat="RB">only</tok> <tok cat="CD">one</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">2</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">presented</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">disease</tok> <tok cat="NN">progression</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
777972609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">PHA-induced</tok> <tok cat="NN">proliferation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">PBMCs</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">breast</tok> <tok cat="NN">cancer</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">depends</tok> <cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">tumour</tok> <tok cat="NN">load</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">good</tok></cons> <cons cat="ADJP"><tok cat="JJ">clinical</tok></cons></cons> <tok cat="NN">predictor</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">further</tok> <tok cat="NN">course</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">disease</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
778192701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Modulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activity</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">intracellular</tok> <tok cat="NN">glutathione</tok> <tok cat="NNS">levels</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">variations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">extracellular</tok> <tok cat="NN">cysteine</tok> <tok cat="NN">supply</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@16-12-2010
778192702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="JJ">HIV-infected</tok> <tok cat="NNS">individuals</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">SIV-infected</tok> <tok cat="NN">rhesus</tok> <tok cat="NNS">macaques</tok></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">average</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><tok cat="VBN">decreased</tok> <cons cat="NP"><tok cat="NN">plasma</tok> <tok cat="NN">cysteine</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">cystine</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">concentrations</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="VBN">decreased</tok> <tok cat="JJ">intracellular</tok> <tok cat="NN">glutathione</tok> <tok cat="NNS">levels</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@16-12-2010
778192703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">depletion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">glutathione</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Molt-4</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">translocation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">incubation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">increasing</tok> <tok cat="JJ">extracellular</tok> <tok cat="NNS">concentrations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cysteine</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">DNA-binding</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">transactivating</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@16-12-2010
778192704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Because</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="VBG">increasing</tok> <tok cat="JJ">intracellular</tok> <tok cat="NN">glutathione</tok> <tok cat="NN">disulfide</tok> <tok cat="NNS">levels</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="NN">GSSG</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="ADVP"><tok cat="RB">directly</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">cell-free</tok> <tok cat="NNS">systems</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">GSSG</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">physiologically</tok> <tok cat="JJ">relevant</tok></cons> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">intact</tok> <tok cat="NNS">cells</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
778192705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="VP"><tok cat="VBZ">controls</tok> <cons cat="NP"><tok cat="JJ">many</tok> <cons cat="NP"><tok cat="RB">immunologically</tok> <tok cat="JJ">important</tok> <tok cat="NNS">genes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="RB">so</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">immune</tok> <tok cat="NN">system</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">sensitive</tok> <cons cat="PP" syn="COOD"><cons cat="CONJP"><tok cat="RB">not</tok> <tok cat="RB">only</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">cysteine</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">glutathione</tok></cons></cons> <tok cat="NN">deficiency</tok></cons></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">also</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">excess</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cysteine</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
778886101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Ah</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TCDD</tok> <tok cat="NN">receptor</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">monocytic</tok> <tok cat="NN">differentiation</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
778886102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">high</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">Ah</tok> <tok cat="NN">receptor</tok> <tok cat="LRB">(</tok><tok cat="NN">TCDD</tok> <tok cat="NN">receptor</tok><tok cat="RRB">)</tok> <tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">individuals</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@16-12-2010
778886103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">paper</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">blood</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">first</tok></cons> <cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">fractions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nucleated</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">revealing</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">predominant</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Ah</tok> <tok cat="NN">receptor</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">monocyte</tok> <tok cat="NN">fraction</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
778886104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Then</tok></cons> <cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AhR</tok> <tok cat="NN">mRNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">various</tok> <cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">together</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Arnt</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">P450IA1</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
778886105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="NN">AhR</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">levels</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">monocytoid</tok> <tok cat="NN">U937</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">THP1</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HEL/S</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="JJ">moderate</tok> <tok cat="NNS">levels</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">promyelocytic</tok> <tok cat="NN">HL60</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">erythroblastic</tok> <tok cat="NN">HEL</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
778886106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">lymphoid</tok> <tok cat="NNS">cells</tok></cons> <cons cat="NP"><tok cat="NN">MOLT4</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">BALL1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">nor</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">K562</tok> <tok cat="NNS">erythroblasts</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
778886107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">specific</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AhR</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NN">differentiation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HL60</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HEL</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
778886108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="NN">HL60</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">differentiate</tok> <cons cat="PP"><tok cat="IN">toward</tok> <cons cat="NP"><tok cat="NNS">monocytes-macrophages</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">incubation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">ester</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">showing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="CD">5-</tok> <tok cat="TO">to</tok> <tok cat="JJ">2-fold</tok></cons> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AhR</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
778886109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">incubation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBG">transforming</tok> <tok cat="NN">growth</tok> <tok cat="NN">factor</tok> <tok cat="NN">beta</tok> <tok cat="CD">1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">1</tok> <tok cat="NN">alpha,25-dihydroxyvitamin</tok> <tok cat="NN">D3</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="CD">5-</tok> <tok cat="TO">to</tok> <tok cat="JJ">7-fold</tok></cons> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AhR</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
778886110@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">HEL</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">exhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">similar</tok> <tok cat="NN">elevation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AhR</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="NN">level</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i19"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="VP"><tok cat="VBN">differentiated</tok> <cons cat="PP"><tok cat="IN">toward</tok> <cons cat="NP"><tok cat="JJ">monocyte-macrophage</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">these</tok> <tok cat="JJ">combined</tok> <tok cat="NNS">inducers</tok></cons></cons><cons cat="ADVP" ref="i19" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" id="i21" role="SBJ"><cons cat="NP"><tok cat="JJ">little</tok> <tok cat="NN">change</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">level</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i21" null="NONE"></cons></cons><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i12"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i13" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="S"><cons cat="NP" ref="i13" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">differentiate</tok> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons><cons cat="ADVP" ref="i12" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@16-12-2010
778886111@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="VBN">differentiated</tok> <tok cat="NN">HL60</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">3-methylcholanthrene</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">ligand</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AhR</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">P450IA1</tok></cons> <tok cat="NN">gene</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
778886112@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBD">indicated</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AhR</tok> <tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i14" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="VBN">differentiated</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">responsive</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NNS">xenobiotics</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
778886113@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">AhR</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">eventual</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">environmental</tok> <tok cat="NNS">carcinogens</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
779151201@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Physiological</tok> <tok cat="NN">concentration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">estradiol</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">polymorphonuclear</tok> <tok cat="NN">leukocyte</tok></cons> <tok cat="NN">chemotaxis</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">receptor</tok> <tok cat="JJ">mediated</tok> <tok cat="NN">system</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@16-12-2010
779151202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Estrogen</tok></cons> <cons cat="VP"><tok cat="VBZ">exhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">variety</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">actions</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">immuno-modulatory</tok> <tok cat="NNS">effects</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP" syn="COOD"><cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@16-12-2010
779151203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">estrogen</tok></cons> <cons cat="VP"><tok cat="VBZ">exerts</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">anti-inflammatory</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons></cons><cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="ADVP"><tok cat="RB">yet</tok></cons> <cons cat="VP"><tok cat="VBN">understood</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
779151204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">possible</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">estradiol</tok></cons> <cons cat="VP"><tok cat="VBG">acting</tok> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">polymorphonuclear</tok> <tok cat="NNS">leukocytes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">PMNs</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
779151205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">agent</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">17</tok> <tok cat="NN">beta-estradiol</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="CD">17</tok> <tok cat="NN">alpha-estradiol</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP"><tok cat="VBD">reduced</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">PMNs</tok></cons> <tok cat="NN">chemotaxis</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">FMLP</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">dose-dependent</tok> <tok cat="NN">manner</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">control</tok></cons> <tok cat="CC">vs</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">estrogen</tok></cons> <cons cat="NP"><cons cat="QP"><tok cat="CD">10(-10)-(-6)</tok></cons> <tok cat="NN">M</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">P</tok></cons> <cons cat="ADJP"><tok cat="JJR">&lt;</tok> <cons cat="NP"><tok cat="CD">0.05</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
779151206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Physiological</tok> <tok cat="NNS">concentrations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">estradiol</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP"><tok cat="VBD">reduced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">chemotaxis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">PMNs</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="QP"><tok cat="CD">10(-10)</tok></cons> <tok cat="NN">mol</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
779151207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Pre-incubation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">clomiphene</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">tamoxifen</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><tok cat="NN">estrogen</tok> <tok cat="NN">receptor</tok> <tok cat="NNS">antagonists</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">eliminated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">17</tok> <tok cat="NN">beta-estradiol</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">chemotaxis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">PMNs</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">restoring</tok> <cons cat="NP"><tok cat="PRP">it</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">control</tok> <tok cat="NN">level</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
779151208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">observations</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="CD">17</tok> <tok cat="NN">beta-estradiol</tok></cons> <cons cat="VP"><tok cat="VBD">suppressed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">chemotaxis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">PMNs</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">receptor-dependent</tok> <tok cat="NN">mechanism</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
779151209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">estradiol</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">plasma</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHPP" id="i8"><cons cat="PP" null="QSTN"></cons><cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="NNS">PMNs</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">drawn</tok><cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP" ref="i8" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">close</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">inverse</tok></cons></cons> <tok cat="NN">correlation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NNS">PMNs</tok></cons> <tok cat="NN">chemotaxis</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">FMLP</tok></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">r</tok></cons> <cons cat="ADJP"><tok cat="JJ">=</tok> <cons cat="NP"><tok cat="CD">-0.821</tok></cons></cons></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">p</tok></cons> <cons cat="ADJP"><tok cat="JJR">&lt;</tok> <cons cat="NP"><tok cat="CD">0.001</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
779151210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Estrogen</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">modify</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">neutrophils</tok></cons></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">normal</tok> <tok cat="JJ">menstrual</tok> <tok cat="NN">cycle</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">not</tok> <tok cat="RB">only</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">pregnancy</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="NN">influence</tok> <cons cat="NP"><tok cat="NN">inflammation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
780264201@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Platelet-activating</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PAF</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">positively</tok></cons> <cons cat="VP"><tok cat="VBZ">auto-regulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">PAF</tok> <tok cat="NN">receptor</tok> <tok cat="NN">transcript</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">leukocyte-type</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@22@@oe@16-12-2010
780264202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">platelet-activating</tok> <tok cat="NN">factor</tok> <tok cat="NN">receptor</tok> <tok cat="LRB">(</tok><tok cat="NN">PAFR</tok><tok cat="RRB">)</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">transcribed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">distinct</tok> <cons cat="NP"><tok cat="NNS">promoters</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="CD">1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="CD">2</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">generate</tok> <cons cat="NP"><cons cat="NP" id="i6"><tok cat="CD">two</tok> <tok cat="NNS">transcripts</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="VP"><tok cat="VBN">designated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PAFR</tok> <tok cat="NN">transcript</tok> <tok cat="CD">1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">PAFR</tok> <tok cat="NN">transcript</tok> <tok cat="CD">2</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">though</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">their</tok> <tok cat="JJ">open</tok> <tok cat="NN">reading</tok> <tok cat="NNS">frames</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">identical</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@46@@oe@16-12-2010
780264203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">primer</tok> <tok cat="NN">extension</tok> <tok cat="NN">analysis</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">discriminate</tok> <cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">transcripts</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">PAFR</tok> <tok cat="NN">transcript</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">leukocyte-type</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">PAFR</tok> <tok cat="NN">transcript</tok> <tok cat="CD">2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">tissue-type</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">upregulated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">PAF</tok></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="NN">12-O-tetradecanoylphorbol-13-acetate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TPA</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">stomach</tok> <tok cat="NN">cancer</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">JR-St</tok> <tok cat="NNS">cells</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">expresses</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADJP"><tok cat="JJ">functional</tok></cons> <cons cat="NP"><tok cat="NN">PAFR</tok> <tok cat="NN">transcript</tok> <tok cat="CD">1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">PAFR</tok> <tok cat="NN">transcript</tok> <tok cat="CD">2</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">endogenously</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@67@@oe@16-12-2010
780264204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Functional</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="CD">1</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">transient</tok> <tok cat="NN">expression</tok> <tok cat="NN">assay</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="NN">chloramphenicol</tok> <tok cat="NN">acetyltransferase</tok> <tok cat="LRB">(</tok><tok cat="NN">CAT</tok><tok cat="RRB">)</tok> <tok cat="NN">gene</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">reporter</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">PAF</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TPA</tok></cons></cons> <cons cat="VP"><tok cat="VBD">activated</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="CD">1</tok></cons></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">deleted</tok> <tok cat="NN">promoter</tok></cons> <cons cat="VP"><tok cat="VBG">lacking</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">three</tok> <cons cat="NP"><tok cat="NN">consensus</tok> <tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="CD">-571</tok> <tok cat="NN">bp</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="CD">-459</tok> <tok cat="NN">bp</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@53@@oe@16-12-2010
780264205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">positive</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PAFR</tok></cons> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">PAF</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">possibly</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">phosphorylation</tok> <tok cat="NN">reaction</tok></cons> <cons cat="VP"><tok cat="VBG">involving</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">PAF</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
780551801@GENIA Treebank@formal@@1@S@<cons cat="NP"><tok cat="LRB">[</tok><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NNS">leukocytes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">asthmatic</tok> <tok cat="NNS">subjects</tok></cons></cons></cons></cons></cons></cons><tok cat="RRB">]</tok></cons>@@@@1@13@@oe@16-12-2010
780551802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GCR</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NNS">leukocytes</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">determined</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">radioligand-binding</tok> <tok cat="NN">assay</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">extrinsic</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">intrinsic</tok></cons></cons> <tok cat="NNS">asthmatics</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
780551803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="PRPP">Their</tok> <tok cat="JJ">corresponding</tok> <tok cat="NN">plasma</tok> <tok cat="NN">cortisol</tok> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">assessed</tok><cons cat="NP" ref="i2" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@16-12-2010
780551804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">average</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GCR</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">asthmatics</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="RB">significantly</tok> <tok cat="JJR">lower</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">subjects</tok></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons> <cons cat="NP" role="PRD"><cons cat="QP"><tok cat="JJR">&lt;</tok> <tok cat="CD">0.01</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">linear</tok> <tok cat="NN">correlation</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GCR</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">course</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">asthma</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
780551805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Besides</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">linear</tok> <tok cat="NN">correlation</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GCR</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">age</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">initial</tok> <tok cat="NN">attack</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">asthma</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
780551806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">No</tok> <tok cat="NN">difference</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">plasma</tok> <tok cat="NN">cortisol</tok> <tok cat="NN">level</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">asthmatics</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">subjects</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
780551807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">no</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">primary</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">general</tok></cons></cons> <tok cat="NN">impairment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">metabolism</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">asthmatics</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GCR</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">asthmatics</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="JJR">lower</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">controls</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
780551808@GENIA Treebank@formal@@1@S@<cons cat="S" id="i4"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GCR</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">asthmatics</tok></cons></cons></cons></cons></cons></cons></cons><cons cat="PRN"><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">think</tok><cons cat="S" ref="i4" null="T"></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">related</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">heredity</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">repeated</tok> <tok cat="NNS">attacks</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">asthma</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
780907201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Identification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TCL1</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T-cell</tok></cons> <tok cat="NNS">malignancies</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@10@@oe@16-12-2010
780907202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">TCL1</tok> <tok cat="NN">locus</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="NN">14q32.1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">frequently</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">chromosomal</tok></cons> <cons cat="NP"><tok cat="NNS">translocations</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">inversions</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">receptor</tok> <tok cat="NNS">loci</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <cons cat="NP"><tok cat="NNS">leukemias</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">lymphomas</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@28@@oe@16-12-2010
780907203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="CD">14</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">translocated</tok></cons> <tok cat="CC">or</tok> <cons cat="VP"><tok cat="VBN">rearranged</tok></cons></cons> <cons cat="NP" null="NONE"></cons></cons><cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="RB">approximately</tok> <tok cat="CD">350</tok></cons> <tok cat="NN">kb</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DNA</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="NN">band</tok> <tok cat="NN">14q32.1</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
780907204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Within</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">gene</tok></cons> <cons cat="VP"><tok cat="VBG">coding</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">1.3-kb</tok> <tok cat="NN">transcript</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">only</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">restricted</tok> <tok cat="NNS">subsets</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">lymphoid</tok> <tok cat="NN">lineage</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">leukemic</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">carrying</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">t(14;14)</tok><tok cat="NN">(q11;q32)</tok> <tok cat="NN">chromosome</tok> <tok cat="NN">translocation</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">inv(14)(q11;q32)</tok> <tok cat="NN">chromosome</tok> <tok cat="NN">inversion</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
780907205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">cognate</tok> <tok cat="NN">cDNA</tok> <tok cat="NN">sequence</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">reveals</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">open</tok> <tok cat="NN">reading</tok> <tok cat="NN">frame</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">342</tok> <tok cat="NN">nt</tok></cons></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="IN">of</tok> <tok cat="CD">14</tok> <tok cat="NN">kDa</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
780907206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">TCL1</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">sequence</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons><cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="PRPP">our</tok> <tok cat="NN">knowledge</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">shows</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">sequence</tok> <tok cat="NN">homology</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">other</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">preferentially</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="ADVP" adv="TMP"><tok cat="RB">early</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">T-</tok> <cons cat="NP" null="QSTN"></cons><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">B-lymphocyte</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
781123101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">Sp1</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">GM-CSF</tok> <tok cat="NN">promoter</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@16-12-2010
781123102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cis-acting</tok> <tok cat="NN">region</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GM-kappa</tok> <tok cat="NN">B/GC-box</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">positions</tok> <tok cat="CD">-95</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-73</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">murine</tok> <tok cat="NN">GM-CSF</tok> <tok cat="NN">gene</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">maximal</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">phorbol-12-myristate</tok> <tok cat="NN">acetate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PMA</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">calcium</tok> <tok cat="NN">ionophore</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">A23187</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@42@@oe@16-12-2010
781123103@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">GM-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="VP"><tok cat="VBZ">defines</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="VBG">binding</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">GC-box</tok></cons> <cons cat="VP"><tok cat="VBZ">defines</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="VBG">binding</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">A1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">A2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">B</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP"><tok cat="JJ">constitutive</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
781123104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">three</tok> <tok cat="NNS">copies</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GC-box</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">functionally</tok></cons> <cons cat="VP"><tok cat="VB">compensate</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GM-kappa</tok> <tok cat="NN">B/GC-box</tok> <tok cat="NN">region</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">GC-motif</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">function</tok> <cons cat="ADVP"><cons cat="ADVP"><tok cat="RB">independently</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GM-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">motif</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
781123105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">major</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">GC-box</tok></cons> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="NP"><tok cat="NN">A1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">purified</tok></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="PP" ref="i31" null="ICH"></cons></cons></cons><cons cat="NP" ref="i8" null="NONE"></cons></cons><cons cat="PP" id="i31"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">Sp1</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
781123106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">depletion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Sp1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">A1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="VP"><tok cat="VBZ">decreases</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">transcription</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GM-CSF</tok></cons> <tok cat="NNS">templates</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
781123107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Since</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">GM-CSF</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">base</tok> <tok cat="NN">difference</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GC-box</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">speculate</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">explain</tok> <cons cat="SBAR"><cons cat="WHADVP" id="i9"><tok cat="WRB">why</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">promoter</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">weak</tok> <cons cat="ADVP" ref="i9" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">upstream</tok> <tok cat="NN">enhancer</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">GM-CSF</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
781129301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">C-terminus</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">activator</tok></cons> <cons cat="NP"><tok cat="NN">Oct-2</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">functions</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">activation</tok> <tok cat="NN">domain</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">yeast</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@16-12-2010
781129302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Oct-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Oct-2</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NNS">activators</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NN">DNA</tok> <tok cat="NN">element</tok></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBP">activate</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">distinct</tok> <tok cat="NNS">sets</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@16-12-2010
781129303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">factors</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="FW">S.</tok> <tok cat="FW">cerevisiae</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJR">greater</tok> <tok cat="IN">than</tok> <tok cat="JJ">5-fold</tok></cons> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">lacZ</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">only</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">Oct-2</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
781129304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Transfer</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Oct-2</tok> <tok cat="JJ">C-terminal</tok> <tok cat="NN">domain</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">onto</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Oct-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Oct1.2</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">nonactivating</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">GAL4</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">created</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">activators</tok></cons> <cons cat="ADJP"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJR">greater</tok> <tok cat="IN">than</tok> <tok cat="CD">15</tok> <tok cat="CC">and</tok> <tok cat="JJ">10-fold</tok></cons> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
781129305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">C-terminus</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Oct-2</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">sufficient</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">confer</tok> <cons cat="NP"><tok cat="NN">activation</tok> <tok cat="NN">potential</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nonactive</tok> <cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NNS">fragments</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">yeast</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
781555501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Characterization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CD48</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">demonstrates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">positive</tok> <tok cat="NN">element</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">specific</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="JJ">virus-immortalized</tok> <tok cat="NN">B-cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">essential</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@25@@oe@16-12-2010
781555502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EBV</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">mature</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="VBG">resting</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">drives</tok> <cons cat="NP" id="i6"><tok cat="PRP">them</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">become</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="NNS">lymphoblasts</tok></cons> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <tok cat="NNS">molecules</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD48</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">characteristically</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="VBN">activated</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@37@@oe@16-12-2010
781555503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">identification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">enhancer</tok> <tok cat="NN">element</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CD48</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">reproducibly</tok></cons> <cons cat="VP"><tok cat="VBZ">confers</tok> <cons cat="NP"><tok cat="JJ">strong</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">EBV-positive</tok> <tok cat="JJ">B-lymphoblastoid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
781555504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">element</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">established</tok> <cons cat="NP"><tok cat="JJ">EBV-negative</tok> <tok cat="NN">B-cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i20" role="SBJ"><tok cat="NN">EBV</tok></cons> <cons cat="VP"><tok cat="VBZ">fails</tok> <cons cat="S"><cons cat="NP" ref="i20" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">drive</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADVP"><tok cat="RB">fully</tok></cons> <cons cat="NP"><tok cat="JJ">lymphoblastoid</tok> <tok cat="NN">phenotype</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
781555505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="DT">An</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">binding</tok> <tok cat="NN">site</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">essential</tok> <tok cat="NN">component</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">element</tok></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="ADVP"><tok cat="RB">alone</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">sufficient</tok></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">account</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">specificity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
781555506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">specific</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">protein</tok> <tok cat="NN">complex</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i11"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">p50</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">part</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
781555507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">EBV-encoded</tok> <tok cat="JJ">latent</tok> <tok cat="NN">membrane</tok> <tok cat="NN">protein</tok> <tok cat="CD">1</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">transactivating</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">isolated</tok> <cons cat="NP"><tok cat="NN">CD48</tok></cons> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">intact</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">latent</tok> <tok cat="NN">membrane</tok> <tok cat="NN">protein</tok> <tok cat="JJ">1-driven</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B/Rel</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">must</tok> <cons cat="VP"><tok cat="VB">interact</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">pathways</tok></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">control</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">EBV</tok></cons> <cons cat="VP"><tok cat="VBZ">drives</tok> <cons cat="NP"><tok cat="VBG">resting</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@56@@oe@16-12-2010
781663401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">An</tok> <cons cat="NP"><tok cat="NN">AP1</tok> <tok cat="NN">binding</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="ADVP"><tok cat="JJ">upstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">kappa</tok> <tok cat="NN">immunoglobulin</tok> <tok cat="NN">intron</tok> <tok cat="NN">enhancer</tok></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="NP"><tok cat="JJ">inducible</tok> <tok cat="NNS">factors</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">contributes</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">expression</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
781663402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">kappa</tok> <tok cat="NN">immunoglobulin</tok> <tok cat="JJ">light</tok> <tok cat="NN">chain</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">developmental-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">tissue-specific</tok></cons></cons> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">trans-acting</tok> <tok cat="NNS">factors</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">interact</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">distinct</tok> <cons cat="NP"><tok cat="NN">enhancer</tok> <tok cat="NNS">elements</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@16-12-2010
781663403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">A</tok> <tok cat="JJ">new</tok> <tok cat="JJ">protein-DNA</tok> <tok cat="NN">interaction</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">upstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">intron</tok> <tok cat="NN">enhancer</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">matrix-associated</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">J-C</tok> <tok cat="NN">intron</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
781663404@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">greatly</tok> <tok cat="JJ">inducible</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">pre-B</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="JJ">bacterial</tok> <tok cat="NN">lipopolysaccharide</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">interleukin-1</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="JJ">specific</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="NNS">stages</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">development</tok></cons> <cons cat="VP"><tok cat="VBN">tested</tok><cons cat="NP" null="NONE"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
781663405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="VBN">footprinted</tok> <tok cat="VBG">binding</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">homologous</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">consensus</tok> <tok cat="NN">AP1</tok> <tok cat="NN">motif</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
781663406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">protein</tok> <tok cat="NNS">components</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">complex</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="VP"><tok cat="VBN">competed</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">AP1</tok> <tok cat="NN">consensus</tok> <tok cat="NN">motif</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">supershift</tok></cons></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">include</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Jun</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Fos</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <cons cat="NP"><tok cat="VBG">binding</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">AP1</tok> <tok cat="NN">motif</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Jun</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Fos</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">families</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">kappa</tok> <tok cat="JJ">light</tok> <tok cat="NN">chain</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@57@@oe@16-12-2010
781663407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="NN">Mutation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">AP1</tok> <tok cat="NN">motif</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">context</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">intron</tok> <tok cat="NN">enhancer</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">decrease</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">enhancer-mediated</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">promoter</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">pre-B</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">LPS</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">constitutive</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
782179801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Identification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">chicken</tok> <tok cat="NN">anemia</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CAV</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <cons cat="NP"><tok cat="JJ">enhancer-like</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@16-12-2010
782179802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">single</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">cloned</tok> <tok cat="NN">genome</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP"><cons cat="NP"><tok cat="NNP">Noteborn</tok> <tok cat="FW">et</tok> <tok cat="FW">al.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">J.</tok> <tok cat="NNP">Virol.</tok> <tok cat="CD">65</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">1991</tok></cons><tok cat="RRB">)</tok></cons> <cons cat="NP"><tok cat="CD">3131-3139</tok></cons></cons><tok cat="RRB">]</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">chicken</tok> <tok cat="NN">anemia</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CAV</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">chicken</tok> <tok cat="NNS">T-cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="JJ">analysed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CAT</tok></cons> <tok cat="NNS">assays</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@37@@oe@16-12-2010
782179803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">unique</tok> <tok cat="NN">region</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><cons cat="ADJP"><cons cat="QP"><tok cat="CD">four</tok> <tok cat="CC">or</tok> <tok cat="CD">five</tok></cons> <tok cat="JJ">near-perfect</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">direct</tok> <tok cat="NNS">repeats</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">DR</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">21</tok> <tok cat="NN">bp</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="CD">one</tok> <cons cat="NP"><tok cat="JJ">12-bp</tok> <tok cat="NN">insert</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">proven</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">main</tok> <cons cat="NP"><tok cat="JJ">transcription-activation</tok> <tok cat="NN">element</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">enhancer-like</tok> <tok cat="NNS">characteristics</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
782179804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PCR</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">CAV</tok> <tok cat="NNS">isolates</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="PP"><tok cat="IN">across</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">world</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="DT">all</tok></cons> <cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP"><tok cat="DT">this</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
782179805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Electrophoretic</tok> <tok cat="JJ">mobility-shift</tok> <tok cat="NNS">assays</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EMSA</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="JJ">individual</tok> <tok cat="NN">DR</tok> <tok cat="NNS">units</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">12-bp</tok> <tok cat="NN">insert</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">factors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">chicken</tok> <tok cat="NNS">T-cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
782179806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Competition</tok> <tok cat="NNS">assays</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">DR</tok> <tok cat="NNS">units</tok></cons></cons> <cons cat="VP"><tok cat="VBD">bound</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">factors</tok></cons> <cons cat="ADJP"><tok cat="JJ">other</tok> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">12-bp</tok> <tok cat="NN">insert</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
782179807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">synthetic</tok> <tok cat="NN">oligodeoxyribonucleotide</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">SP1-box</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">5'-GGGCGG</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">compete</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">factors</tok></cons> <cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">12-bp</tok> <tok cat="NN">insert</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
782179808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="VBN">Purified</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">SP1</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">very</tok> <tok cat="JJ">strong</tok></cons> <tok cat="NN">affinity</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">12-bp</tok> <tok cat="NN">insert</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
782280901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">thymocytes</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">EBV</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@9@@oe@16-12-2010
782280902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">discovery</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">EBV</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">certain</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">malignancies</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">EBV</tok> <tok cat="NN">receptor</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CR2/CD21</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">population</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">immature</tok> <tok cat="NNS">thymocytes</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="JJ">lymphoblastoid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">childhood</tok> <tok cat="JJ">acute</tok> <tok cat="NN">T</tok> <tok cat="JJ">lymphoblastic</tok> <tok cat="NN">leukemia</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">suggested</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">EBV-receptor</tok> <tok cat="NNS">interactions</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="PP" role="PRD"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">importance</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@50@@oe@16-12-2010
782280903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">thymus</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">population</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">large</tok><tok cat="COMMA">,</tok> <tok cat="JJ">immature</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">expresses</tok> <cons cat="NP"><tok cat="NN">CD21</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
782280904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">EBV</tok></cons> <cons cat="VP"><tok cat="VBD">altered</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok> <tok cat="NNS">responses</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">immature</tok> <tok cat="NNS">thymocytes</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
782280905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="NOM" role="SBJ"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Triggering</tok> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="NN">CD2</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">mitogenic</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">mature</tok></cons><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">immature</tok></cons><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i20"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
782280906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">EBV</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">ligation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD2</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">thymocytes</tok></cons> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">proliferate</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">absence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">exogenous</tok> <tok cat="NNS">cytokines</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
782280907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">function</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">result</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">EBV</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">receptor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CD21</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">caused</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">infection</tok></cons> <cons cat="CONJP"><tok cat="RB">rather</tok> <tok cat="IN">than</tok></cons> <cons cat="NP"><tok cat="NN">surface</tok> <tok cat="NN">signaling</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">because</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">neither</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">mAb</tok></cons> <tok cat="CC">nor</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">P3HR-1</tok> <tok cat="NN">strain</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">virus</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">mimicked</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">B95-8</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
782280908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="JJ">Immature</tok> <cons cat="NP"><tok cat="NNS">thymocytes</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">infected</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">EBV</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">determined</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">internalization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">genome</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
782280909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADJP"><tok cat="JJ">Consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">B95-8</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><tok cat="NN">EBNA-2</tok> <tok cat="NNS">transcripts</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="JJ">infected</tok> <tok cat="NN">thymocyte</tok> <tok cat="NNS">populations</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
782280910@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="NNS">components</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">viral</tok> <tok cat="NN">replicative</tok> <tok cat="NN">pathway</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">thymocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
782280911@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">components</tok></cons> <cons cat="VP"><tok cat="VBD">included</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">BZLF-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">characterizes</tok> <cons cat="NP"><tok cat="JJ">EBV-infected</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">disruption</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">latency</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
782280912@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="DT">A</tok> <tok cat="JJ">second</tok> <tok cat="NN">transcript</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">recently</tok> <tok cat="VBD">characterized</tok></cons> <cons cat="NP"><tok cat="NN">RAZ</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i13"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i13" id="i14" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i14" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">replicative</tok> <tok cat="NN">infection</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
782280913@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">consequences</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">EBV</tok> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">early</tok> <tok cat="NN">stage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">lead</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">failure</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">normal</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <cons cat="NP"><tok cat="NN">repertoire</tok> <tok cat="NN">development</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">autoimmunity</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">malignancy</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
782394301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Distinct</tok> <tok cat="NNS">roles</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <cons cat="NP"><tok cat="NN">chaperone</tok> <tok cat="NN">hsp90</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">modulating</tok> <cons cat="NP"><tok cat="NN">dioxin</tok> <tok cat="NN">receptor</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">basic</tok> <cons cat="NP"><tok cat="JJ">helix-loop-helix</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">PAS</tok> <tok cat="NNS">domains</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@16-12-2010
782394302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">dioxin</tok> <tok cat="NN">receptor</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">mediates</tok> <cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">dioxin</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">functions</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">ligand-activated</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@16-12-2010
782394303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">basic</tok> <tok cat="JJ">helix-loop-helix</tok> <tok cat="LRB">(</tok><tok cat="JJ">bHLH</tok><tok cat="RRB">)</tok> <tok cat="NN">motif</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">contiguous</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">Per-Arnt-Sim</tok> <tok cat="LRB">(</tok><tok cat="NN">PAS</tok><tok cat="RRB">)</tok> <tok cat="NN">homology</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
782394304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">nonstimulated</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="NP" id="i5" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">receptor</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">recovered</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">inducible</tok></cons> <cons cat="ADJP"><tok cat="JJ">cytoplasmic</tok></cons></cons> <tok cat="NN">form</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">90-kDa</tok> <tok cat="NN">heat</tok> <tok cat="NN">shock</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">hsp90</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">molecular</tok> <tok cat="NN">chaperone</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
782394305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">reconstituted</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">ligand-dependent</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">receptor</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NN">form</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">dioxin</tok> <tok cat="NN">receptor</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <cons cat="NP"><tok cat="JJ">bHLH-PAS</tok> <tok cat="NN">partner</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="NP"><tok cat="NN">Arnt</tok></cons></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">translation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">reticulocyte</tok> <tok cat="NN">lysate</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
782394306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Deletion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">PAS</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">constitutive</tok> <tok cat="NN">dimerization</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">Arnt</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
782394307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">receptor</tok> <tok cat="NN">mutant</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">low</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">xenobiotic</tok> <tok cat="NN">response</tok> <tok cat="JJ">element-binding</tok> <tok cat="NN">activity</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">PAS</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="JJ">DNA-binding</tok> <cons cat="NP"><tok cat="NN">affinity</tok></cons> <tok cat="CC">and/or</tok> <cons cat="NP"><tok cat="NN">specificity</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
782394308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRP">It</tok></cons> <cons cat="S" ref="i7" null="EXP"></cons></cons><cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">possible</tok> <cons cat="S" id="i7"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">reconstitute</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">dioxin</tok> <tok cat="NN">receptor</tok></cons> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">wheat</tok> <tok cat="NN">germ</tok> <tok cat="NN">lysate</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
782394309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">line</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">observations</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">reticulocyte</tok> <tok cat="NN">lysate</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">wheat</tok> <tok cat="NN">germ</tok> <tok cat="NN">lysate</tok></cons></cons> <cons cat="VP"><tok cat="VBD">promoted</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">association</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="FW">de</tok> <tok cat="FW">novo</tok></cons> <tok cat="VBN">synthesized</tok> <cons cat="NP"><tok cat="NN">dioxin</tok> <tok cat="NN">receptor</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">hsp90</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
782394310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="QP"><tok cat="IN">At</tok> <tok cat="JJS">least</tok> <tok cat="CD">two</tok></cons> <tok cat="JJ">distinct</tok> <tok cat="NNS">domains</tok> <cons cat="NP" ref="i8" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">receptor</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">mediated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">hsp90</tok></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD" id="i8"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">ligand-binding</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">PAS</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">surprisingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">bHLH</tok> <tok cat="NN">domain</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
782394311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">ligand-binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBD">correlated</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">association</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">hsp90</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="JJ">bHLH-hsp90</tok> <tok cat="NN">interaction</tok></cons> <cons cat="VP"><tok cat="VBD">appeared</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NN">activity</tok></cons></cons> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">dimerization</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
782394312@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">Several</tok> <tok cat="JJ">distinct</tok></cons> <tok cat="NNS">roles</tok> <cons cat="NP" ref="i10" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">hsp90</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">modulating</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">dioxin</tok> <tok cat="NN">receptor</tok></cons> <tok cat="NN">function</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">likely</tok></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD" id="i10"><cons cat="NP"><cons cat="NP"><tok cat="JJ">correct</tok> <tok cat="NN">folding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">ligand-binding</tok> <tok cat="NN">domain</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interference</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Arnt</tok></cons> <tok cat="NN">heterodimerization</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">folding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">conformation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">bHLH</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
782394313@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">dioxin</tok> <tok cat="NN">receptor</tok></cons> <tok cat="NN">system</tok></cons> <cons cat="VP"><tok cat="VBZ">provides</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">complex</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">interesting</tok></cons></cons> <tok cat="NN">model</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">hsp90</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
782662301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="JJ">functional</tok> <tok cat="NNS">regions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">IL-3</tok> <tok cat="JJ">proximal</tok> <tok cat="NN">promoter</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@10@@oe@16-12-2010
782662302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">interleukin-3</tok> <tok cat="LRB">(</tok><tok cat="NN">IL-3</tok><tok cat="RRB">)</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">almost</tok> <tok cat="RB">exclusively</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@16-12-2010
782662303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="PRPP">Its</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <tok cat="DT">the</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">transcriptional</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">post-transcriptional</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">level</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
782662304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">phytohemaglutinin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PHA</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phorbol</tok> <tok cat="NN">ester</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">PMA</tok></cons><tok cat="COMMA">,</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">activated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">initiation</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-3</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
782662305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">define</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">regions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">gene</tok></cons> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">generated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">series</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">reporter</tok> <tok cat="NNS">constructs</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">regions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-3</tok> <tok cat="NN">gene</tok> <tok cat="JJ">5'</tok> <tok cat="CC">and</tok> <tok cat="JJ">3'</tok> <tok cat="JJ">flanking</tok> <tok cat="NNS">sequences</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
782662306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i8" role="SBJ"><tok cat="CC">Both</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">positive</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">negative</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="JJ">regulatory</tok> <tok cat="NNS">elements</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">proximal</tok> <tok cat="JJ">5'</tok> <tok cat="JJ">flanking</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-3</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
782662307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="CD">-173</tok> <tok cat="CC">and</tok> <tok cat="CD">-60</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJS">strongest</tok> <cons cat="NP"><tok cat="VBG">activating</tok> <tok cat="NNS">elements</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
782662308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">positive</tok> <tok cat="NN">activator</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
782662309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-3</tok> <tok cat="NN">CK-1/CK-2</tok> <tok cat="NNS">elements</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">many</tok> <cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">acted</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">repressor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">basal</tok> <tok cat="NN">level</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i10"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">cloned</tok> <cons cat="ADVP"><tok cat="RB">upstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">heterologous</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="ADVP" ref="i10" null="T"></cons></cons></cons></cons></cons><tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">inducible</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">PMA/PHA</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
782667001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Alternate</tok> <tok cat="JJ">immune</tok> <tok cat="NN">system</tok> <tok cat="VBZ">targets</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">TCDD</tok></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">lymphocyte</tok> <tok cat="NN">stem</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">extrathymic</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">development</tok></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
782667002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="VP"><tok cat="VBP">summarize</tok> <cons cat="NP"><tok cat="NN">evidence</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i26" role="SBJ"><cons cat="NP"><tok cat="JJ">thymic</tok> <tok cat="NN">atrophy</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">2,3,7,8-tetrachlorodibenzo-p-dioxin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TCDD</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">mediated</tok><cons cat="NP" ref="i26" null="NONE"></cons><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">part</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">damage</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">extrathymic</tok> <tok cat="NN">T-cell</tok> <tok cat="NNS">precursors</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">bone</tok> <tok cat="NN">marrow</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">fetal</tok> <tok cat="NN">liver</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
782667003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">atrophy</tok> <tok cat="NN">induction</tok></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">involve</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">apoptotic</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">thymocytes</tok></cons> <cons cat="VP"><tok cat="VBN">affected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="NN">bcl-2</tok> <tok cat="NN">proto-oncogene</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
782667004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">TCDD</tok></cons> <cons cat="VP"><tok cat="VBZ">mediates</tok> <cons cat="NP"><tok cat="NN">atrophy</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">specific</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">AhR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">estrogen</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
782667005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">Both</tok> <cons cat="NP"><tok cat="NN">TCDD</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">estradiol</tok></cons></cons> <cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">extrathymic</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">liver</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
782667006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="JJ">extrathymic</tok> <tok cat="NN">T-cell</tok> <tok cat="NNS">populations</tok></cons> <cons cat="VP"><tok cat="VBP">include</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">elevated</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">V</tok> <tok cat="NN">beta</tok> <tok cat="NN">T-cell</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i27"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i27" id="i28" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">normally</tok></cons> <tok cat="VBN">deleted</tok> <cons cat="NP" ref="i28" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">thymic</tok> <tok cat="NN">development</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
782859901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">B-cell</tok></cons> <cons cat="NP"><tok cat="NN">proliferation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">induction</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">early</tok> <cons cat="NP"><tok cat="JJ">G1-regulating</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok> <tok cat="NNS">mutants</tok></cons> <cons cat="ADJP"><tok cat="JJ">conditional</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">EBNA2</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@16-12-2010
782859902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NNS">B-lymphocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EBV</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">growth</tok> <tok cat="NN">transformation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NNS">B-cells</tok></cons></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@16-12-2010
782859903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">EBV</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">antigen</tok> <tok cat="CD">2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EBNA2</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">EBV</tok> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">B-cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="CC">and</tok> <tok cat="JJ">cellular</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBG">transforming</tok> <tok cat="NN">potential</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">virus</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
782859904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">generated</tok> <cons cat="NP"><tok cat="JJ">conditional</tok> <tok cat="NN">EBV</tok> <tok cat="NNS">mutants</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP"><tok cat="NN">EBNA2</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">chimeric</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">hormone</tok> <tok cat="NN">binding</tok> <tok cat="NN">domain</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">estrogen</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">genetic</tok> <tok cat="NN">background</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">virus</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
782859905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Growth</tok> <tok cat="NN">transformation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">normal</tok> <tok cat="NNS">B-cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">mutant</tok> <tok cat="NN">virus</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">estrogen-dependent</tok> <tok cat="JJ">lymphoblastoid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">chimeric</tok> <cons cat="NP"><tok cat="NN">EBNA2</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
782859906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">absence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">estrogen</tok></cons></cons></cons></cons> <cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="QP"><tok cat="RB">about</tok> <tok cat="NN">half</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">enter</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">quiescent</tok></cons> <cons cat="ADJP"><tok cat="JJ">non-proliferative</tok></cons></cons> <tok cat="NN">state</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">others</tok></cons> <cons cat="VP"><tok cat="VBP">die</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
782859907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">EBNA2</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="CONJP"><tok cat="RB">not</tok> <tok cat="RB">only</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">initiation</tok></cons> <cons cat="PP" ref="i21" null="RNR"></cons></cons><cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">also</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">maintenance</tok></cons> <cons cat="PP" ref="i21" null="RNR"></cons></cons><cons cat="PP" id="i21"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transformation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
782859908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Growth</tok> <tok cat="NN">arrest</tok></cons> <cons cat="VP"><tok cat="VBD">occurred</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">G1</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">G2</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NNS">stages</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="JJ">functional</tok> <cons cat="NP"><tok cat="NN">EBNA2</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NN">restriction</tok> <tok cat="NNS">points</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
782859909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Growth</tok> <tok cat="NN">arrest</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">reversible</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">G1/G0</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">indicated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="JJ">sequential</tok></cons> <cons cat="NP"><tok cat="NN">accumulation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">modification</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">cycle</tok> <tok cat="NN">regulating</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
782859910@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">EBV</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok> <tok cat="NN">regulating</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">polyclonal</tok> <tok cat="NNS">stimuli</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <cons cat="NP"><tok cat="NNS">B-cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
782859911@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i16" role="SBJ"><tok cat="NN">EBV</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">common</tok> <tok cat="NN">pathway</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i16" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">bypassing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">requirement</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">antigen</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NNS">signals</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">growth</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
783337001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Evaluation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">respiratory</tok> <tok cat="NN">epithelium</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">normals</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">individuals</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">cystic</tok> <tok cat="NN">fibrosis</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">adenovirus</tok> <tok cat="NN">E1a</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="ADJP"><tok cat="JJ">relevant</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">use</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">E1a-</tok> <tok cat="NN">adenovirus</tok> <tok cat="NNS">vectors</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">therapy</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">respiratory</tok> <tok cat="NNS">manifestations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">cystic</tok> <tok cat="NN">fibrosis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@38@@oe@16-12-2010
783337002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Lung</tok> <tok cat="NN">disease</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">disorders</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cystic</tok> <tok cat="NN">fibrosis</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CF</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">amenable</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">somatic</tok> <tok cat="NN">gene</tok> <tok cat="NN">therapy</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="NN">delivery</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">directly</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">respiratory</tok> <tok cat="NN">epithelium</tok></cons></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">E1a-</tok> <tok cat="NN">adenovirus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Ad</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">2-</tok> <cons cat="ADJP" null="QSTN"></cons><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><cons cat="ADJP"><tok cat="JJ">5-based</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">vectors</tok></cons></cons></cons></cons><cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@45@@oe@16-12-2010
783337003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">For</tok> <cons cat="NP"><tok cat="NN">safety</tok> <tok cat="NNS">reasons</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">Ad</tok> <tok cat="NNS">vectors</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">rendered</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="ADJP" role="PRD"><tok cat="NN">replication</tok> <tok cat="JJ">deficient</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">deletion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">E1a</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
783337004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Because</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">theoretical</tok> <tok cat="NN">possibility</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ"><tok cat="DT">an</tok> <tok cat="JJ">E1a-</tok> <tok cat="JJ">replication-deficient</tok> <tok cat="NN">vector</tok></cons> <cons cat="VP"><tok cat="VBG">replicating</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">result</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">recombination</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">complementation</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Ad</tok> <tok cat="CD">2/5</tok> <tok cat="NN">E1a</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="ADJP"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">target</tok> <tok cat="NN">cell</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">directed</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">toward</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">evaluating</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">respiratory</tok> <tok cat="NN">epithelium</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">normals</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">individuals</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">CF</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">E1a</tok> <tok cat="NNS">sequences</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@16-12-2010
783337005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Ad</tok> <tok cat="CD">2/5</tok> <tok cat="JJ">E1a-specific</tok> <tok cat="NNS">primers</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">polymerase</tok> <tok cat="NN">chain</tok> <tok cat="NN">reaction</tok></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">evaluate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">DNA</tok></cons> <cons cat="VP"><tok cat="VBN">recovered</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="ADJP"><tok cat="RB">freshly</tok> <tok cat="VBN">isolated</tok></cons> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">nasal</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">bronchial</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NN">epithelium</tok></cons></cons> <cons cat="VP"><tok cat="VBN">recovered</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">brushing</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="NN">E1a</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">respiratory</tok> <tok cat="NN">epithelium</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">19</tok> <tok cat="IN">of</tok> <tok cat="CD">91</tok></cons> <tok cat="NNS">normals</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">21</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
783337006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">E1a-positive</tok> <tok cat="NNS">samples</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">average</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">E1a</tok> <tok cat="NN">copy</tok> <tok cat="NN">number</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="QP"><tok cat="CD">55</tok> <tok cat="CC">+/-</tok> <tok cat="CD">18/10(3)</tok></cons> <tok cat="VBN">recovered</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
783337007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">CF</tok> <tok cat="NNS">individuals</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="QP"><tok cat="CD">7</tok> <tok cat="IN">of</tok> <tok cat="CD">52</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">13</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="JJ">detectable</tok> <cons cat="NP"><tok cat="NN">E1a</tok> <tok cat="NNS">sequences</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">respiratory</tok> <tok cat="NN">epithelium</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">E1a</tok> <tok cat="NN">copy</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">positive</tok> <tok cat="NNS">samples</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="QP"><tok cat="CD">80</tok> <tok cat="CC">+/-</tok> <tok cat="CD">21/10(3)</tok></cons> <tok cat="VBN">recovered</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
783337008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><tok cat="JJ">detectable</tok> <cons cat="NP"><tok cat="NN">Ad</tok> <tok cat="CD">2/5</tok> <tok cat="NN">E1a</tok> <tok cat="NNS">sequences</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">respiratory</tok> <tok cat="NN">epithelium</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">small</tok> <tok cat="NN">percentage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">normals</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">individuals</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">CF</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
783337009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Because</tok> <tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">theoretical</tok> <tok cat="NN">potential</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ"><tok cat="JJ">such</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="VP"><tok cat="VBG">supporting</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">replication</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">E1a-</tok> <tok cat="NN">Ad</tok> <tok cat="NNS">vectors</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">gene</tok> <tok cat="NN">therapy</tok> <tok cat="NNS">protocols</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">CF</tok></cons></cons> <cons cat="VP"><tok cat="VBG">utilizing</tok> <cons cat="NP"><tok cat="JJ">such</tok> <tok cat="NNS">vectors</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">should</tok> <cons cat="VP"><tok cat="VB">consider</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">evaluating</tok> <cons cat="NP"><tok cat="NN">study</tok> <tok cat="NNS">individuals</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Ad</tok> <tok cat="CD">2/5</tok> <tok cat="NN">E1a</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">respiratory</tok> <tok cat="NN">epithelium</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
783638901@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="CD">Two</tok> <tok cat="JJ">distinct</tok> <tok cat="NN">signalling</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">biphasic</tok> <cons cat="NP"><tok cat="NN">junB</tok></cons> <tok cat="NN">transcription</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">interleukin-6</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">hybridoma</tok> <tok cat="NN">7TD1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@16-12-2010
783638902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">measured</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">junB</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">hybridoma</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">7TD1</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-6</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-6</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">stimulation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@16-12-2010
783638903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-6</tok></cons> <cons cat="VP"><tok cat="VBZ">increases</tok> <cons cat="NP"><tok cat="NN">junB</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">biphasic</tok> <tok cat="NN">fashion</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
783638904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">first</tok></cons> <cons cat="ADJP"><tok cat="JJ">early-induced</tok></cons></cons> <tok cat="NN">peak</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">transient</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">likely</tok></cons> <cons cat="VP"><tok cat="VBZ">corresponds</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="RB">well</tok> <tok cat="VBN">documented</tok></cons> <cons cat="ADJP"><tok cat="JJ">typical</tok></cons></cons> <cons cat="NP"><tok cat="NN">junB</tok> <tok cat="NN">mRNA</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">numerous</tok> <cons cat="NP"><tok cat="NN">growth</tok> <tok cat="NNS">factors</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><tok cat="NN">IL-6</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
783638905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">At</tok> <cons cat="NP"><tok cat="NN">variance</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">second</tok> <tok cat="NN">peak</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" id="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="ADVP"><tok cat="RB">never</tok></cons> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">reported</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">lasted</tok> <cons cat="NP" role="PRD"><tok cat="JJ">several</tok> <tok cat="NNS">hours</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
783638906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">As</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">consequence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">junB</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">IL-6</tok></cons> <cons cat="VP"><tok cat="VBD">stimulated</tok><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">biphasic</tok> <tok cat="NN">fashion</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">accumulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">JunB</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
783638907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">IL-6</tok></cons> <tok cat="NN">regulation</tok></cons> <cons cat="VP"><tok cat="VBD">occurred</tok> <cons cat="ADVP"><tok cat="RB">exclusively</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">level</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">bimodal</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">junB</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">JunB</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">accounted</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">biphasic</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">junB</tok></cons> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
783638908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">point</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">major</tok> <tok cat="NNS">differences</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="JJ">early</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="JJ">late</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="JJ">IL-6-induced</tok> <cons cat="NP"><tok cat="NN">junB</tok></cons> <tok cat="NN">transcription</tok> <tok cat="NNS">waves</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
783638909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">First</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">cycloheximide</tok></cons> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBD">potentiated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">second</tok> <tok cat="NN">wave</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">failed</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">early-induced</tok> <tok cat="NN">burst</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
783638910@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Second</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">tyrphostin</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok> <tok cat="NN">inhibitor</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">impaired</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="JJ">first</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="JJ">second</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><cons cat="NP"><tok cat="NN">junB</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="NN">peak</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
783638911@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Conversely</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">genistein</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">another</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok> <tok cat="NN">inhibitor</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="ADVP"><tok cat="RB">totally</tok></cons> <cons cat="VP"><tok cat="VBD">abolished</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">second</tok> <tok cat="NN">peak</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">junB</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NN">peak</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
783638912@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Altogether</tok></cons> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok><cons cat="S"><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">7TD1</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">IL-6</tok></cons> <cons cat="VP"><tok cat="VBZ">controls</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">junB</tok></cons> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">biphasic</tok> <tok cat="NN">fashion</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">means</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">separate</tok> <tok cat="NN">transduction</tok> <tok cat="NNS">pathways</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
784323001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Biphasic</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activation</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">receptor</tok> <tok cat="NN">complex</tok></cons></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">alpha</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@16-12-2010
784323002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="LRB">(</tok><tok cat="NN">NF</tok><tok cat="RRB">)</tok><tok cat="NN">-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">receptor</tok> <tok cat="LRB">(</tok><tok cat="NN">TcR</tok><tok cat="RRB">)</tok><tok cat="NN">/CD3</tok> <tok cat="NN">complex</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">human</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">studied</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NNS">times</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@35@@oe@16-12-2010
784323003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADVP"><tok cat="RB">Only</tok></cons> <cons cat="NP"><tok cat="NN">p50</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="VP"><tok cat="VBD">bound</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">element</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">interleukin-2</tok> <tok cat="NN">receptor</tok> <tok cat="LRB">(</tok><tok cat="NN">IL-2R</tok><tok cat="RRB">)</tok> <tok cat="NN">alpha</tok> <tok cat="NN">chain</tok> <tok cat="NN">promoter</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="VBG">resting</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
784323004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">immediately</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">TcR/CD3</tok></cons> <tok cat="JJ">cross-linking</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="PP"><cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="QP"><tok cat="RB">approximately</tok> <tok cat="CD">1</tok></cons> <tok cat="NN">h</tok></cons></cons><tok cat="COLON">;</tok> <cons cat="ADJP"><tok cat="JJ">immediate</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p50.p65</tok> <tok cat="NNS">heterodimers</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok><cons cat="NP" ref="i6" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
784323005@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="NN">p50.c-rel</tok> <tok cat="NNS">heterodimers</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">bound</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">sequence</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">time</tok> <tok cat="NNS">points</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">7-16</tok></cons> <tok cat="NN">h</tok></cons><tok cat="COLON">;</tok> <cons cat="ADJP"><tok cat="RB">early</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">both</tok></cons> <cons cat="VP"><tok cat="VBD">remained</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">active</tok> <cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJR">later</tok> <tok cat="NN">time</tok> <tok cat="NNS">points</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="CD">40</tok> <tok cat="NN">h</tok></cons><tok cat="COLON">;</tok> <cons cat="ADJP"><tok cat="RB">late</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
784323006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">regulation</tok></cons> <cons cat="VP"><tok cat="VBZ">takes</tok> <cons cat="NP"><tok cat="NN">place</tok></cons> <cons cat="PP" syn="COOD"><cons cat="ADVP"><tok cat="RB">mainly</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p65</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-rel</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">immediate</tok></cons> <cons cat="NP" ref="i24" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">early</tok></cons> <cons cat="NP" ref="i24" null="RNR"></cons></cons><cons cat="NP" id="i24"><tok cat="NN">time</tok> <tok cat="NNS">points</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
784323007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Activation</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-rel</tok> <cons cat="NP" ref="i29" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p105/p50</tok> <cons cat="NP" ref="i29" null="RNR"></cons></cons><cons cat="NP" id="i29"><tok cat="NN">mRNA</tok> <tok cat="NN">synthesis</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">p65</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WPP">whose</tok> <tok cat="NN">expression</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">constitutive</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
784323008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Interestingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i9" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="PDT">all</tok> <tok cat="DT">those</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">early</tok></cons> <cons cat="NP" ref="i25" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">late</tok></cons> <cons cat="NP" ref="i25" null="RNR"></cons></cons><cons cat="NP" id="i25"><tok cat="NNS">events</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immediate</tok> <tok cat="NNS">ones</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">a</tok> <tok cat="VBG">neutralizing</tok> <cons cat="NP"><tok cat="JJ">anti-tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">alpha</tok> <tok cat="LRB">(</tok><tok cat="NN">TNF-alpha</tok><tok cat="RRB">)</tok> <tok cat="JJ">monoclonal</tok> <tok cat="NN">antibody</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
784323009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Similarly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">cycloheximide</tok></cons> <cons cat="VP"><tok cat="VBD">prevented</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">p65</tok></cons> <cons cat="NP" ref="i26" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-rel</tok></cons> <cons cat="NP" ref="i26" null="RNR"></cons></cons><cons cat="NP" id="i26"><tok cat="NN">translocation</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">consequent</tok> <tok cat="NN">formation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">active</tok> <tok cat="NN">binding</tok> <tok cat="NNS">heterodimers</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">early</tok></cons> <cons cat="NP" ref="i27" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">late</tok></cons> <cons cat="NP" ref="i27" null="RNR"></cons></cons><cons cat="NP" id="i27"><tok cat="NNS">times</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
784323010@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Cyclosporin</tok> <tok cat="NN">A</tok></cons> <cons cat="VP"><tok cat="VBD">impaired</tok> <cons cat="NP" syn="COOD"><cons cat="CONJP"><tok cat="RB">not</tok> <tok cat="RB">only</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">early</tok></cons> <cons cat="NP" ref="i28" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">late</tok></cons><cons cat="NP" ref="i28" null="RNR"></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">also</tok></cons> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">immediate</tok></cons> <cons cat="NP" ref="i28" null="RNR"></cons></cons><cons cat="NP" id="i28"><tok cat="NNS">events</tok></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">prevented</tok> <cons cat="NP"><tok cat="DT">all</tok> <tok cat="NN">inhibition</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
784323011@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activation</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">TcR/CD3</tok></cons> <tok cat="NNS">signals</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">biphasic</tok></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">TcR/CD3</tok></cons> <cons cat="VP"><tok cat="VBZ">triggers</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">immediate</tok> <tok cat="NN">translocation</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">transient</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">if</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">no</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
784323012@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i16" role="SBJ"><tok cat="NN">TNF-alpha</tok><tok cat="COMMA">,</tok></cons> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">emerges</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">main</tok> <tok cat="NN">factor</tok></cons> <cons cat="ADJP"><tok cat="JJ">responsible</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">second</tok> <tok cat="NN">phase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">regulation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i16" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">controlling</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p65</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-rel</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">new</tok> <cons cat="NP"><tok cat="NN">mRNA</tok></cons> <tok cat="NN">synthesis</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-rel</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p105/p50</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
784325101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">downstream</tok> <tok cat="NN">effector</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p21ras</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
784325102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">aim</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="S" role="PRD"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">investigate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PKC</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="JJ">downstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p21ras</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">activating</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-2</tok> <tok cat="LRB">(</tok><tok cat="NN">IL-2</tok><tok cat="RRB">)</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@32@@oe@16-12-2010
784325103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">reported</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PKC</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">effector</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p21ras</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
784325104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NNS">Data</tok> <cons cat="SBAR" ref="i30" null="ICH"></cons></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">presented</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">potent</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">selective</tok></cons></cons> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">PKC</tok></cons> <tok cat="NN">inhibitor</tok></cons> <cons cat="NP"><tok cat="NN">Ro</tok> <tok cat="CD">31-8425</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">constitutively</tok> <tok cat="JJ">active</tok></cons> <tok cat="NN">ras</tok> <tok cat="JJ">mutant</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" id="i30"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">clearly</tok></cons> <cons cat="VP"><tok cat="VBZ">shows</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PKC</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="ADVP"><tok cat="JJ">downstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p21ras</tok></cons></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@54@@oe@16-12-2010
784325105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Reporter</tok> <tok cat="NN">gene</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">affected</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <cons cat="NP"><tok cat="NN">p21ras</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
784325106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="VP"><tok cat="VBG">involving</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">PKC</tok></cons> <tok cat="NN">activation</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">calcium</tok> <tok cat="NN">mobilization</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">ras</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">combine</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">necessary</tok> <tok cat="NNS">components</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
784867901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Association</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">alterations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NNS">moieties</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok> <tok cat="JJ">proviral</tok> <tok cat="NN">latency</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">certain</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@16-12-2010
784867902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HIV-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">replication</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">controlled</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">complex</tok> <tok cat="NN">array</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">virally</tok> <tok cat="VBN">encoded</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">cellular</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@22@@oe@16-12-2010
784867903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">wide</tok> <tok cat="NN">spectrum</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="UCP" syn="COOD"><tok cat="CC">both</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">culture</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
784867904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Molecular</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">restricted</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">replication</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">differ</tok> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="JJ">certain</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
784867905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="JJ">proviral</tok> <tok cat="NN">latency</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">U1</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">only</tok> <tok cat="RB">extremely</tok> <tok cat="JJ">low</tok></cons> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">baseline</tok> <tok cat="JJ">unstimulated</tok> <tok cat="NN">state</tok></cons></cons><cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">alterations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <cons cat="NP"><tok cat="NN">factor-kappa</tok> <tok cat="NN">B</tok> <tok cat="LRB">(</tok><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok><tok cat="RRB">)</tok> <tok cat="NNS">moieties</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">EMSAs</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">situ</tok></cons> <tok cat="NN">UV</tok> <tok cat="JJ">cross-linking</tok> <tok cat="NNS">studies</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@64@@oe@16-12-2010
784867906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">predominance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p50</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NNS">moieties</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">possibly</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p50</tok> <tok cat="NNS">homodimers</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">closely</tok> <tok cat="JJ">related</tok></cons> <tok cat="NNS">species</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">rather</tok> <tok cat="IN">than</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">p50-p56</tok> <tok cat="NN">heterodimer</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">the</tok> <tok cat="JJ">predominant</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NNS">species</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="ADJP"><tok cat="JJS">most</tok></cons> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">T</tok> <tok cat="JJ">lymphocytic</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">monocytic</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">nuclei</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">U1</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@48@@oe@16-12-2010
784867907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">This</tok> <tok cat="NN">pattern</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="JJ">B-related</tok> <tok cat="NNS">moieties</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">differs</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="RB">latently</tok> <tok cat="JJ">infected</tok> <tok cat="NN">T</tok> <tok cat="JJ">lymphocytic</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">ACH-2</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">U937</tok> <tok cat="JJ">monocytic</tok> <tok cat="NN">line</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">parental</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">U1</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">clone</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
784867908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">As</tok> <cons cat="NP"><tok cat="JJ">such</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">different</tok></cons> <cons cat="ADJP"><tok cat="JJ">proximal</tok></cons></cons> <tok cat="NNS">mechanisms</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">lead</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">restricted</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">replication</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
784892101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Overexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C-zeta</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">leukemic</tok> <tok cat="NN">cell</tok></cons> <tok cat="NN">differentiation</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@10@@oe@16-12-2010
784892102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C-zeta</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PKC-zeta</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">member</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phorbol</tok> <tok cat="NN">ester</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">nonresponsive</tok></cons> <cons cat="ADJP"><tok cat="JJ">atypical</tok></cons></cons> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok> <tok cat="NN">subfamily</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">modulating</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">leukemic</tok> <tok cat="NN">U937</tok> <tok cat="NN">cell</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@34@@oe@16-12-2010
784892103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="VBN">Transfected</tok> <cons cat="NP"><tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">stably</tok></cons> <tok cat="VBG">overexpressing</tok> <cons cat="NP"><tok cat="NN">PKC-zeta</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">displayed</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADVP"><tok cat="RBR">longer</tok></cons> <tok cat="JJ">doubling</tok> <tok cat="NN">time</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJR">lower</tok> <tok cat="NN">saturation</tok> <tok cat="NN">density</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="NN">confluency</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">adherence</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">plastic</tok></cons></cons></cons></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" ref="i14" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">control</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
784892104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PKC-zeta</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RBR">more</tok> <tok cat="VBN">differentiated</tok></cons> <tok cat="NN">phenotype</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">assessed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">morphology</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">surface</tok> <tok cat="NN">antigen</tok> <tok cat="NN">expression</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">lysosomal</tok> <tok cat="NN">enzyme</tok></cons> <tok cat="NNS">activities</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">distinct</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">parental</tok> <tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">differentiate</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">exposure</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NNS">esters</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
784892105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">contrast</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">parental</tok> <cons cat="NP"><tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">PKC-zeta</tok> <tok cat="NNS">cells</tok></cons> <cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP"><tok cat="NN">mRNA</tok> <tok cat="NNS">transcripts</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-jun</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">low</tok> <tok cat="NN">mobility</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
784892106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">PKC-zeta</tok></cons> <tok cat="NN">overexpression</tok></cons> <cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">type</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">phenotypic</tok> <tok cat="NN">differentiation</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">differs</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">maturation</tok></cons> <cons cat="VP"><tok cat="VBG">occurring</tok> <cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">other</tok> <cons cat="NP"><tok cat="NN">PKC</tok> <tok cat="NNS">subfamilies</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">phorbol</tok> <tok cat="NN">ester</tok> <tok cat="NN">treatment</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
784892107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="VBN">Increased</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">c-jun</tok></cons> <tok cat="NN">protooncogene</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">PKC-zeta</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">provides</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potential</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">explaining</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">altered</tok> <tok cat="NN">differentiation</tok> <tok cat="NN">status</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">cell</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
784929101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Posttranscriptional</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">macrophage</tok> <cons cat="NP"><tok cat="NN">tissue</tok> <tok cat="NN">factor</tok></cons> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NNS">antioxidants</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@10@@oe@16-12-2010
784929102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Tissue</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TF</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">monocyte/macrophage</tok> <tok cat="NN">lineage</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">represents</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="VP"><tok cat="VBG">underlying</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">initiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">fibrin</tok></cons> <tok cat="NN">deposition</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">extravascular</tok> <tok cat="NN">inflammation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@16-12-2010
784929103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Recent</tok> <tok cat="NN">evidence</tok></cons> <cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">oxidant</tok> <tok cat="NN">stress</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">signalling</tok> <tok cat="NN">pathway</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">virtue</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">various</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
784929104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">antioxidant</tok> <tok cat="NN">treatment</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">lipopolysaccharide</tok> <tok cat="LRB">(</tok><tok cat="NN">LPS</tok><tok cat="RRB">)</tok><tok cat="JJ">-induced</tok> <cons cat="NP"><tok cat="NN">TF</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">murine</tok> <tok cat="JJ">peritoneal</tok> <tok cat="NNS">macrophages</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
784929105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">Both</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">pyrrolidine</tok> <tok cat="NN">dithiocarbamate</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">oxidant</tok> <tok cat="NN">scavenger</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">N-acetyl-cysteine</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">precursor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">endogenous</tok> <tok cat="JJ">antioxidant</tok></cons> <cons cat="NP"><tok cat="NN">glutathione</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">macrophage</tok> <tok cat="NN">procoagulant</tok> <tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">LPS</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
784929106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Northern</tok> <tok cat="NN">blot</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">neither</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">agents</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">reduced</tok> <cons cat="NP"><tok cat="JJ">LPS-stimulated</tok> <cons cat="NP"><tok cat="NN">TF</tok></cons> <tok cat="NN">mRNA</tok> <tok cat="NN">accumulation</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">thereby</tok></cons> <cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">posttranscriptional</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
784929107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Immunofluorescence</tok> <tok cat="NNS">studies</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="JJ">polyclonal</tok> <cons cat="NP"><tok cat="JJ">anti-TF</tok> <tok cat="NN">antibody</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">N-acetyl-cysteine</tok> <tok cat="NN">treatment</tok></cons> <cons cat="VP"><tok cat="VBD">prevented</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">characteristic</tok> <tok cat="JJ">plasmalemmal</tok> <tok cat="NN">localization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TF</tok> <tok cat="NN">antigen</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">LPS</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
784929108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Western</tok> <tok cat="NN">blot</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">N-acetyl-cysteine</tok></cons> <cons cat="VP"><tok cat="VBD">reduced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">accumulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">47-kD</tok> <tok cat="JJ">mature</tok> <tok cat="NN">glycoprotein</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">LPS-treated</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">finding</tok></cons> <cons cat="ADJP"><tok cat="JJ">consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">results</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">immunofluorescence</tok> <tok cat="NNS">studies</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
784929109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">conditions</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">result</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">accumulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RBR">less</tok> <tok cat="JJ">mature</tok></cons> <tok cat="NNS">forms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TF</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
784929110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i8"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">considered</tok> <cons cat="NP" ref="i16" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">together</tok></cons><cons cat="ADVP" ref="i8" null="T"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i16" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="NNS">antioxidants</tok></cons> <cons cat="VP"><tok cat="VBP">exert</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">impairing</tok> <cons cat="NP"><tok cat="NN">translation</tok></cons></cons></cons></cons> <tok cat="CC">and/or</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">causing</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">degradation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="RB">newly</tok> <cons cat="NP"><tok cat="VBN">translated</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
784929111@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">antioxidants</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">appeared</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="NNS">species</tok> <tok cat="JJ">specific</tok><tok cat="COMMA">,</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">LPS-induced</tok> <tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">murine</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
784929112@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">posttranscriptional</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">antioxidants</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">TF</tok></cons> <tok cat="NN">expression</tok> <tok cat="NNS">data</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i12"><tok cat="WRB">whereby</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">agents</tok></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">modulate</tok> <cons cat="NP"><tok cat="NN">monocyte/macrophage</tok> <tok cat="NN">activation</tok></cons><cons cat="ADVP" ref="i12" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
784954301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">1,25-Dihydroxyvitamin</tok> <tok cat="NN">D3</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">primary</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">secondary</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">hyperparathyroidism</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@16-12-2010
784954302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="VBN">decreased</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">calcitriol</tok> <tok cat="LRB">(</tok><tok cat="NN">1,25(OH)2D3</tok><tok cat="RRB">)</tok> <tok cat="NNS">receptors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">parathyroid</tok> <tok cat="NNS">glands</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">uremic</tok> <tok cat="NNS">animals</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
784954303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NNS">humans</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBN">carried</tok> <cons cat="PRT"><tok cat="RP">out</tok></cons> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">surgically</tok> <tok cat="VBN">removed</tok></cons> <tok cat="JJ">parathyroid</tok> <tok cat="NNS">glands</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">calcitriol</tok> <tok cat="NN">binding</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="JJR">higher</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">primary</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons></cons><cons cat="PP"><tok cat="IN">than</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">secondary</tok></cons> <cons cat="NP" id="i21"><tok cat="NN">hyperparathyroidism</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
784954304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Since</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">calcitriol</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">described</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PBMC</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">specific</tok> <tok cat="NN">uptake</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">3H-labelled</tok> <tok cat="NN">1,25(OH)2D3</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PBMC</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="CD">12</tok> <tok cat="NNS">women</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">hyperparathyroidism</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PHP</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">8</tok> <tok cat="NNS">women</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">hyperparathyroidism</tok> <cons cat="ADJP"><tok cat="JJ">secondary</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">chronic</tok> <tok cat="JJ">renal</tok></cons> <tok cat="NN">failure</tok></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SH</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">9</tok> <tok cat="NNS">women</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">renal</tok> <tok cat="NN">transplant</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RT</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">23</tok> <tok cat="JJ">healthy</tok> <tok cat="NNS">women</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@65@@oe@16-12-2010
784954305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">median</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">dissociation</tok> <tok cat="NN">constant</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Kd</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">similar</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="CD">three</tok> <tok cat="NNS">groups</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">patients</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">women</tok></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">mean</tok> <tok cat="CC">+/-</tok> <tok cat="NN">S.D.</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">range</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PHP</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">1.2</tok> <tok cat="CC">+/-</tok> <tok cat="CD">1.0</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">0.2-4</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">x</tok> <cons cat="NP"><tok cat="CD">10(-10)</tok> <tok cat="NN">M</tok></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">SH</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">0.6</tok> <tok cat="CC">+/-</tok> <tok cat="CD">0.4</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">0.2-1.2</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">x</tok> <cons cat="NP"><tok cat="CD">10(-10)</tok> <tok cat="NN">M</tok></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">RT</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">1.1</tok> <tok cat="CC">+/-</tok> <tok cat="CD">0.5</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">0.4-1.9</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">x</tok> <cons cat="NP"><tok cat="CD">10(-10)</tok> <tok cat="NN">M</tok></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">controls</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">1.0</tok> <tok cat="CC">+/-</tok> <tok cat="CD">0.6</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">0.3-2.6</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">x</tok> <cons cat="NP"><tok cat="CD">10(-10)</tok> <tok cat="NN">M</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@76@@oe@16-12-2010
784954306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">maximal</tok> <tok cat="NN">binding</tok> <tok cat="NN">capacity</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Nmax</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><cons cat="VP"><tok cat="VBN">enhanced</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">PHP</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">3.9</tok> <tok cat="CC">+/-</tok> <tok cat="CD">1.9</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">1.3-7.6</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">fmol/10(7)</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">vs.</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">2.3</tok> <tok cat="CC">+/-</tok> <tok cat="CD">0.9</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">1.1-4.4</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">fmol/10(7)</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">controls</tok></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">P</tok></cons> <cons cat="ADJP"><tok cat="JJ">=</tok> <cons cat="NP"><tok cat="CD">0.0006</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons cat="VP"><tok cat="VBN">decreased</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">SH</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">0.8</tok> <tok cat="CC">+/-</tok> <tok cat="CD">0.5</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">0.2-1.6</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">fmol/10(7)</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">vs.</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">2.3</tok> <tok cat="CC">+/-</tok> <tok cat="CD">0.9</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">1.1-4.4</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">fmol/10(7)</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">controls</tok></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">P</tok></cons> <cons cat="ADJP"><tok cat="JJ">=</tok> <cons cat="NP"><tok cat="CD">0.0001</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="DT">no</tok> <tok cat="NNS">changes</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">seen</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">RT</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">2.3</tok> <tok cat="CC">+/-</tok> <tok cat="CD">0.7</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">1.2-3.3</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">fmol/10(7)</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">vs.</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">2.3</tok> <tok cat="CC">+/-</tok> <tok cat="CD">0.9</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">1.1-4.4</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">fmol/10(7)</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">controls</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@98@@oe@16-12-2010
784954307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">PHP</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i12"><tok cat="WP">who</tok></cons> <cons cat="S"><cons cat="NP" ref="i12" id="i13" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">subjected</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">parathyroidectomy</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">calcitriol</tok> <tok cat="NN">number</tok></cons> <cons cat="VP"><tok cat="VBD">came</tok> <cons cat="PRT"><tok cat="RB">down</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="ADJP"><tok cat="JJ">normal</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
784954308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Changes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">calcitriol</tok> <tok cat="NNS">receptors</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">primary</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">secondary</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NN">hyperparathyroidism</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">magnify</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">consequences</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">disturbances</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">serum</tok> <tok cat="NN">concentration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">calcitriol</tok> <tok cat="PRP">itself</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">development</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">secondary</tok> <tok cat="NN">hyperparathyroidism</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">uremia</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
784962801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">Effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">antisense</tok> <tok cat="NN">myb</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">hemin-</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">erythropoietin- induced</tok> <tok cat="JJ">erythroid</tok> <tok cat="NN">differentiation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">K562</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@16-12-2010
784962802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">In</tok> <tok cat="NN">order</tok> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">elucidate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-myb</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">K562</tok> <tok cat="NN">cell</tok></cons></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="NN">hemin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Hm</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">erythropoietin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Epo</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">constructed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">recombinant</tok> <tok cat="NN">plasmid</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">produce</tok> <cons cat="NP"><tok cat="JJ">antisense</tok> <tok cat="NN">myb</tok> <tok cat="NN">RNA</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">dexamethasone</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@42@@oe@16-12-2010
784962803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">During</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">Hm</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">K562</tok> <tok cat="NNS">cells</tok></cons> <cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP"><tok cat="NN">c-myb</tok> <tok cat="NN">mRNA</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="CD">50</tok><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRP">them</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">began</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">synthesize</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">hemoglobin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Hb</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
784962804@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">antisense</tok> <tok cat="NN">myb</tok> <tok cat="NN">RNA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">reduced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">amount</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-myb</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">percentage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">Hb-synthesizing</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">decreased</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="CD">20</tok><tok cat="NN">%</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
784962805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Epo</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">c-myb</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="VP"><tok cat="VBD">declined</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">20</tok><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">K562</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">synthesized</tok> <cons cat="NP"><tok cat="NN">Hb</tok></cons> <cons cat="ADVP"><tok cat="JJ">regardless</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">antisense</tok> <tok cat="NN">myb</tok> <tok cat="NN">RNA</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
784962806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">suggested</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">constitutive</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-myb</tok> <tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">necessary</tok> <cons cat="PP"><tok cat="IN">for</tok> <tok cat="JJ">Hm-induced</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">amount</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-myb</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">antisense</tok> <tok cat="NN">myb</tok> <tok cat="NN">RNA</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">suppresses</tok> <cons cat="NP"><tok cat="JJ">Hm-induced</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
784962807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">amount</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-myb</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">K562</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">reduced</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">Epo</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
784962808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok> <tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">almost</tok> <tok cat="JJ">constant</tok> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">Hm-induced</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Epo</tok></cons> <tok cat="NN">treatment</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
784962809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">supposed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">Hm-induced</tok> <tok cat="NN">differentiation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">distinguished</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Epo-induced</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">K562</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
785203201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">Alteration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">structural</tok> <tok cat="NN">order</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">erythrocyte</tok> <tok cat="NN">ghost</tok> <tok cat="NN">membrane</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">influence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok> <tok cat="NN">antagonist</tok> <tok cat="NN">RU</tok> <tok cat="CD">486</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
785203202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i18" role="SBJ"><tok cat="JJ">High-dose</tok> <tok cat="NN">pulse</tok> <tok cat="NN">glucocorticoid</tok> <tok cat="NN">therapy</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i18" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">successfully</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">clinic</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">severe</tok> <tok cat="JJ">pathological</tok> <tok cat="NNS">conditions</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="QP"><tok cat="RB">about</tok> <tok cat="CD">20</tok></cons> <tok cat="NNS">years</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
785203203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">mode</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">action</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">administration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">such</tok> <tok cat="NNS">megadoses</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">inexplicable</tok></cons> <cons cat="PP" adv="TMP"><tok cat="RP">up</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="RB">now</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
785203204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">supposed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">some</tok> <tok cat="NNS">effects</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="PP" role="PRD"><tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">membrane</tok> <tok cat="NNS">alterations</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
785203205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <cons cat="ADJP"><tok cat="FW">in-vitro</tok></cons> <tok cat="NNS">experiments</tok></cons></cons> <cons cat="NP" id="i19" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">dexamethasone</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">further</tok> <tok cat="NNS">glucocorticoids</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok> <tok cat="NN">antagonist</tok> <tok cat="NN">RU</tok> <tok cat="CD">486</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">structural</tok> <tok cat="NN">order</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">erythrocyte</tok> <tok cat="NN">ghost</tok> <tok cat="NNS">membranes</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="NP" ref="i19" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">determining</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">steady-state</tok> <tok cat="NN">fluorescence</tok> <tok cat="NN">anisotropy</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">diphenylhexatriene</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">DPH</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
785203206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i20" role="SBJ"><tok cat="NN">Dexamethasone</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i20" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">significant</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">membrane</tok> <tok cat="JJ">structural</tok> <tok cat="NN">order</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">concentrations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="QP"><tok cat="RB">about</tok> <tok cat="CD">10(-6)</tok></cons> <tok cat="NN">M</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">concentration-dependent</tok> <tok cat="NN">manner</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
785203207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">correlation</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">uptake</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">dexamethasone</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ghost</tok> <tok cat="NNS">membranes</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">structural</tok> <tok cat="NN">order</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
785203208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">other</tok> <tok cat="NNS">glucocorticoids</tok></cons> <cons cat="VP"><tok cat="VBN">tested</tok><cons cat="NP" null="NONE"></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">methylprednisolone</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">corticosterone</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">effective</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">concentrations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">10(-5)</tok> <tok cat="NN">M</tok> <cons cat="QP"><tok cat="CC">or</tok> <tok cat="JJR">greater</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
785203209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">change</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">membrane</tok> <tok cat="JJ">structural</tok> <tok cat="NN">order</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">RU</tok> <tok cat="CD">486</tok></cons> <cons cat="PP"><tok cat="IN">up</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">concentration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">10(-4)</tok> <tok cat="NN">M</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
785203210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">simultaneous</tok> <tok cat="NN">incubation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">RU</tok> <tok cat="CD">486</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">dexamethasone</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">distinct</tok> <tok cat="NN">interference</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">RU</tok> <tok cat="CD">486</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">dexamethasone</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
785203211@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">glucocorticoid-induced</tok> <tok cat="NN">membrane</tok> <tok cat="NN">perturbation</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">possibility</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><tok cat="PRP">it</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">RU</tok> <tok cat="CD">486</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">inactivity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">structurally</tok> <tok cat="JJ">related</tok></cons> <tok cat="NN">progesterone</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">refer</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">relatively</tok> <tok cat="JJ">specific</tok></cons> <tok cat="VBG">binding</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">glucocorticoids</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">membrane</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">erythrocyte</tok> <tok cat="NNS">ghosts</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
785348301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p105</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">persistently</tok></cons> <tok cat="VBN">infected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
785348302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HIV</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">persistence</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes/macrophages</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">partially</tok></cons> <cons cat="VP"><tok cat="VBN">understood</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@16-12-2010
785348303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Persistent</tok> <tok cat="NN">HIV</tok> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">U937</tok> <tok cat="JJ">monocytic</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
785348304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" role="SBJ"><tok cat="IN">Whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">virus-induced</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">favors</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">continuous</tok> <tok cat="JJ">viral</tok> <tok cat="NN">replication</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">remains</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unknown</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
785348305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="ADVP"><tok cat="RB">further</tok></cons> <cons cat="VP"><tok cat="VB">delineate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">HIV-infected</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">focused</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
785348306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">First</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">persistent</tok> <tok cat="NN">HIV</tok> <tok cat="NN">infection</tok></cons> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="CONJP"><tok cat="RB">not</tok> <tok cat="RB">only</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">also</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
785348307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">HIV-infected</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons> <tok cat="NN">protein</tok> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">decreased</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="ADJP"><tok cat="JJ">secondary</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="VBN">enhanced</tok> <tok cat="NN">protein</tok> <tok cat="NN">degradation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
785348308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBZ">parallels</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">increased</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons> <tok cat="NN">synthesis</tok></cons> <cons cat="ADJP"><tok cat="JJ">secondary</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">increased</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="FW">i.e.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="VBN">increased</tok> <cons cat="NP"><tok cat="NN">RNA</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">transcriptional</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">promoter-enhancer</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
785348309@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">Another</tok> <tok cat="NN">protein</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">function</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p105</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">modified</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">HIV-infected</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i11" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">p105</tok></cons> <cons cat="NP" ref="i25" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">p50</tok></cons> <cons cat="NP" ref="i25" null="RNR"></cons></cons><cons cat="NP" id="i25"><tok cat="JJ">steady-state</tok> <tok cat="NN">protein</tok> <tok cat="NNS">levels</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">increased</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">result</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">synthesis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">proteolytic</tok> <tok cat="NN">processing</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p105</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
785348310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="JJ">Transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p105</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">increased</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">infected</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">specific</tok> <cons cat="NP"><tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">motif</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
785348311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">existence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">triple</tok> <tok cat="JJ">autoregulatory</tok> <tok cat="NN">loop</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBG">involving</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HIV</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p105</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p50</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">MAD3</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">end</tok> <tok cat="NN">result</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">persistent</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">persistence</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
785348312@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">persistent</tok> <tok cat="NN">HIV</tok> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">provides</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">useful</tok> <tok cat="NN">model</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="IN">with</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">study</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">concomitant</tok> <tok cat="NNS">modifications</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">different</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons><cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
785826101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Detection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="CD">16</tok> <tok cat="NN">CBF</tok> <tok cat="NN">beta-MYH11</tok> <tok cat="NN">fusion</tok> <tok cat="NN">transcript</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">myelomonocytic</tok> <tok cat="NNS">leukemias</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
785826102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Karyotypic</tok> <tok cat="NN">detection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">chromosomal</tok> <tok cat="CD">16</tok> <tok cat="NNS">abnormalities</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">classically</tok></cons> <tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">AML</tok> <tok cat="NN">M4Eo</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">difficult</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
785826103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Characterization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">inv(16)(p13q22)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CBF</tok> <tok cat="NN">beta</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">MYH11</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">allowed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">detection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">fusion</tok> <tok cat="NNS">transcripts</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">reverse-transcriptase</tok> <tok cat="NN">polymerase</tok> <tok cat="NN">chain</tok> <tok cat="NN">reaction</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RT-PCR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
785826104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP"><tok cat="NN">CBF</tok> <tok cat="NN">beta-MYH11</tok> <tok cat="NN">fusion</tok> <tok cat="NNS">transcripts</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">RT-PCR</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">myelomonocytic</tok> <tok cat="NNS">leukemias</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">eosinophilia</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">their</tok> <tok cat="NN">presence</tok></cons> <cons cat="VP"><tok cat="VBZ">correlates</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">morphology</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
785826105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="CD">Fifty-three</tok> <cons cat="NP" id="i21"><tok cat="NNS">cases</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">11</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP"><tok cat="NN">AML</tok> <tok cat="NN">M4Eo</tok></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">1</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons><cons cat="NP"><cons cat="NP"><tok cat="NN">AML</tok> <tok cat="NN">M4</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">atypical</tok> <tok cat="JJ">abnormal</tok> <tok cat="NNS">eosinophils</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AML</tok> <tok cat="NN">M4</tok> <tok cat="LQT">"</tok><tok cat="NN">Eo</tok><tok cat="RQT">"</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">29</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP"><tok cat="NN">AML</tok> <tok cat="NN">M4</tok></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">8</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP"><tok cat="NN">AML</tok> <tok cat="NN">M5</tok></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">3</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP"><tok cat="NN">CMML</tok></cons></cons><tok cat="COLON">;</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">1</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP"><cons cat="NP"><tok cat="NN">AML</tok> <tok cat="NN">M2</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">eosinophilia</tok></cons></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok><cons cat="NP" ref="i8" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
785826106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">All</tok> <tok cat="CD">11</tok> <tok cat="JJ">typical</tok> <tok cat="NN">AML</tok> <tok cat="NN">M4Eo</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="NN">CBF</tok> <tok cat="NN">beta-MYH11</tok> <tok cat="JJ">positive</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
785826107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">single</tok> <tok cat="NN">case</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AML</tok> <tok cat="NN">M4</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">distinctive</tok> <tok cat="NN">eosinophil</tok> <tok cat="NNS">abnormalities</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">negative</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">karyotype</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">RT-PCR</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">fluorescent</tok> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">situ</tok></cons> <tok cat="NN">hybridization</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">FISH</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
785826108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">Three</tok> <cons cat="SBAR" ref="i9" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">29</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">10</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">AML</tok> <tok cat="NN">M4</tok></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP"><tok cat="JJ">abnormal</tok> <tok cat="NNS">eosinophils</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="NN">CBF</tok> <tok cat="NN">beta-MYH11</tok> <tok cat="JJ">positive</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" id="i9"><cons cat="WHNP" id="i10"><cons cat="NP"><tok cat="CD">1</tok></cons> <cons cat="WHPP"><tok cat="IN">of</tok></cons> <tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">show</tok> <cons cat="NP"><tok cat="DT">any</tok> <tok cat="JJ">apparent</tok> <cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="CD">16</tok></cons> <tok cat="NNS">abnormalities</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">classical</tok> <tok cat="NN">metaphase</tok> <tok cat="NN">analysis</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="CD">2</tok></cons> <cons cat="VP"><tok cat="RB">not</tok> <tok cat="VBN">tested</tok><cons cat="NP" null="NONE"></cons></cons></cons><tok cat="RRB">)</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
785826109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">Both</tok> <tok cat="NNS">cases</tok></cons> <cons cat="VP"><tok cat="VBN">tested</tok> <cons cat="NP" null="NONE"></cons></cons></cons><cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">MYH11</tok></cons> <tok cat="JJ">genomic</tok> <tok cat="NN">rearrangement</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
785826110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">None</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">other</tok> <tok cat="NNS">leukemias</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="NN">RT-PCR</tok> <tok cat="JJ">positive</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
785826111@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Follow-up</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">three</tok> <tok cat="NN">patient</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">residual</tok> <tok cat="NN">positivity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">apparent</tok></cons> <cons cat="ADJP"><tok cat="JJ">complete</tok></cons></cons> <tok cat="NN">remission</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
785826112@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">CBF</tok> <tok cat="NN">beta-MYH11</tok> <tok cat="NN">fusion</tok> <tok cat="NNS">transcripts</tok></cons> <cons cat="VP"><tok cat="VBP">occur</tok> <cons cat="PP" syn="COOD"><cons cat="CONJP"><tok cat="RB">not</tok> <tok cat="RB">only</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">vast</tok> <tok cat="NN">majority</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">typical</tok> <tok cat="NN">AML</tok> <tok cat="NN">M4Eo</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">also</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="RB">approximately</tok> <tok cat="CD">10</tok></cons><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AML</tok> <tok cat="NN">M4</tok></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP"><tok cat="JJ">eosinophilic</tok> <tok cat="NNS">abnormalities</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">much</tok> <tok cat="JJR">higher</tok></cons> <tok cat="NN">incidence</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">than</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">sporadic</tok> <tok cat="NNS">reports</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="CD">16</tok></cons> <tok cat="NNS">abnormalities</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">AML</tok> <tok cat="NN">M4</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">would</tok> <cons cat="VP"><tok cat="VB">suggest</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@16-12-2010
785826113@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">Taken</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">together</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">detection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CBF</tok> <tok cat="NN">beta-MYH11</tok> <tok cat="NNS">transcripts</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">absence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">apparent</tok> <cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="CD">16</tok></cons> <tok cat="NNS">abnormalities</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">classical</tok> <tok cat="NN">banding</tok> <tok cat="NNS">techniques</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="JJ">additional</tok> <tok cat="NN">screening</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="NN">RT-PCR</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">FISH</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">should</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">performed</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">all</tok> <tok cat="NN">AML</tok> <tok cat="NN">M4</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">regardless</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">morphologic</tok> <tok cat="NNS">features</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">allow</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">accurate</tok> <tok cat="NN">evaluation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">prognostic</tok> <tok cat="NN">importance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <cons cat="NP"><tok cat="NN">fusion</tok> <tok cat="NN">transcript</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@59@@oe@16-12-2010
785849101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">genomic</tok> <tok cat="NN">configuration</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">GM-CSF</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-3</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">M-CSF</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">C-FMS</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">growth</tok> <tok cat="NN">response</tok> <tok cat="NN">gene-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EGR-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">M-CSF</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">myelodysplastic</tok> <tok cat="NNS">syndromes</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@30@@oe@16-12-2010
785849102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="JJ">Peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">seventeen</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">myelodysplastic</tok> <tok cat="NNS">syndromes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MDS</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">advanced</tok> <tok cat="NN">stage</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="UCP" syn="COOD"><cons cat="ADJP" role="PRD"><tok cat="JJ">enriched</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NNS">blasts</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">tested</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">1</tok><tok cat="RRB">)</tok></cons> <tok cat="NN">karyotype</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">2</tok><tok cat="RRB">)</tok></cons> <tok cat="JJ">genomic</tok> <tok cat="NN">configuration</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">3</tok><tok cat="RRB">)</tok></cons> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-3</tok><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">GM-CSF</tok><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">FMS</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">EGR-1</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">genes</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="DT">all</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">long</tok> <tok cat="NN">arm</tok> <tok cat="IN">of</tok> <tok cat="NN">chromosome</tok> <tok cat="CD">5</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@60@@oe@16-12-2010
785849103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">M-CSF</tok> <tok cat="NN">gene</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" id="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP"><tok cat="VBN">reassigned</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">short</tok> <tok cat="NN">arm</tok> <tok cat="IN">of</tok> <tok cat="NN">chromosome</tok> <tok cat="CD">1</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">lp</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">investigated</tok><cons cat="NP" ref="i6" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
785849104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Aims</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">study</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="S" role="PRD"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">1</tok><tok cat="RRB">)</tok></cons> <tok cat="VB">assess</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">potential</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">maintenance</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">expansion</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">neoplastic</tok> <tok cat="NNS">clones</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">2</tok><tok cat="RRB">)</tok></cons> <tok cat="VB">search</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">constitutional</tok></cons> <cons cat="NP"><tok cat="NNS">losses</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NNS">rearrangements</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">one</tok> <cons cat="NP"><tok cat="NN">allele</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">followed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">deletion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">second</tok> <cons cat="NP"><tok cat="NN">allele</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NNS">genes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">leukemic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@58@@oe@16-12-2010
785849105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i19" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">latter</tok> <tok cat="NN">issue</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="NP" ref="i19" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">comparing</tok><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="CD">8</tok> <tok cat="NNS">cases</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">constitutive</tok> <tok cat="NN">DNA</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">skin</tok> <tok cat="NNS">fibroblasts</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">leukemic</tok> <tok cat="NN">DNA</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
785849106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">Eleven</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">17</tok> <tok cat="NNS">patients</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="JJ">abnormal</tok> <tok cat="NNS">karyotypes</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
785849107@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">M-CSF</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="CD">6</tok> <tok cat="NNS">cases</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i12" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">FMS</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">EGR-1</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">2</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">latter</tok> <tok cat="NNS">cases</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
785849108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i13" role="SBJ"><cons cat="NP"><tok cat="DT">An</tok> <tok cat="JJ">autocrine</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">growth</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">hypothesized</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">only</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">2</tok> <tok cat="NNS">patients</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i14"><tok cat="WPP">whose</tok> <tok cat="NNS">cells</tok></cons> <cons cat="S"><cons cat="NP" ref="i14" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">M-CSF</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">FMS</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
785849109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i15" role="SBJ"><tok cat="DT">No</tok> <tok cat="NN">germline</tok> <cons cat="NP"><tok cat="NNS">changes</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NNS">rearrangements</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">any</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBN">studied</tok><cons cat="NP" null="NONE"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
785849110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i16" role="SBJ"><cons cat="NP"><tok cat="NN">deregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">certain</tok> <cons cat="NP"><tok cat="JJ">hemopoietic</tok> <tok cat="NN">growth</tok> <tok cat="NNS">factors</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">seem</tok> <cons cat="S"><cons cat="NP" ref="i16" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">represent</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">major</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MDS</tok> <tok cat="NN">progression</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
785929001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">OBF-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">novel</tok> <tok cat="NN">B</tok> <tok cat="JJ">cell-specific</tok> <tok cat="NN">coactivator</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">immunoglobulin</tok> <tok cat="NN">promoter</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">association</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">octamer-binding</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@16-12-2010
785929002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="ADJP"><tok cat="JJ">Recent</tok></cons> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">biochemical</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">genetic</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">studies</tok></cons></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">octamer-binding</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Oct-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Oct-2</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="JJ">other</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NNS">components</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">lymphoid-restricted</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="NN">octamer</tok> <tok cat="JJ">site-mediated</tok></cons></cons> <cons cat="NP"><tok cat="NN">immunoglobulin</tok> <tok cat="NN">gene</tok> <tok cat="NN">promoter</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@33@@oe@16-12-2010
785929003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i14" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">genetic</tok> <tok cat="NN">screen</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">yeast</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i14" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">B</tok> <tok cat="JJ">cell-derived</tok> <tok cat="NNS">cDNAs</tok></cons> <cons cat="VP"><tok cat="JJ">encoding</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Oct-binding</tok> <tok cat="NN">factor</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">OBF-1</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="NN">protein</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i17"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i17" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="VP"><tok cat="VBZ">associates</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Oct-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Oct-2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
785929004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Biochemical</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">OBF-1</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="JJ">intrinsic</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">activity</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">recognizes</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">POU</tok> <tok cat="NNS">domains</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Oct-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Oct-2</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Oct-4</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Oct-6</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
785929005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">OBF-1</tok> <tok cat="NN">mRNA</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">highly</tok> <tok cat="JJ">cell-specific</tok></cons> <tok cat="NN">manner</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">being</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><cons cat="ADJP"><tok cat="RBS">most</tok> <tok cat="JJ">abundant</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">essentially</tok></cons> <cons cat="ADJP"><tok cat="JJ">absent</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJS">most</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <tok cat="JJ">other</tok> <cons cat="NP"><tok cat="NNS">cells</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NNS">tissues</tok></cons></cons> <cons cat="VP"><tok cat="VBN">tested</tok><cons cat="NP" null="NONE"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
785929006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">OBF-1</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">HeLa</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">selectively</tok></cons> <cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">natural</tok> <cons cat="NP"><tok cat="NN">immunoglobulin</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">octamer</tok> <tok cat="JJ">site-dependent</tok> <tok cat="NN">manner</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
785929007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">OBF-1</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><cons cat="NP"><tok cat="PDT">all</tok> <tok cat="DT">the</tok> <tok cat="NNS">properties</tok></cons> <cons cat="VP"><tok cat="VBN">expected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="JJ">cell-specific</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">coactivator</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
785973501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i15" role="SBJ"><cons cat="NP"><tok cat="JJ">Glucocorticoid-induced</tok> <tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">leukemic</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">caused</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">repressive</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@16-12-2010
785973502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">account</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">therapeutic</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NNS">diseases</tok></cons> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><tok cat="NN">leukemia</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">depends</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@16-12-2010
785973503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">events</tok></cons> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">activated</tok> <tok cat="NN">receptor</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">lysis</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">understood</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
785973504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">A</tok> <tok cat="VBG">prevailing</tok> <tok cat="NN">hypothesis</tok></cons> <cons cat="VP"><tok cat="VBZ">postulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">so-called</tok> <tok cat="LQT">'</tok><cons cat="NP"><tok cat="NN">lysis</tok> <tok cat="NNS">genes</tok></cons><tok cat="RQT">'</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
785973505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">activation-deficient</tok> <tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok> <tok cat="NN">mutant</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">as</tok> <tok cat="JJ">effective</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">wild-type</tok> <tok cat="NN">receptor</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">repression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-2</tok></cons> <tok cat="NN">production</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-myc</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
785973506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok> <tok cat="NN">receptor</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WPP">whose</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">repressive</tok> <tok cat="NNS">functions</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">target</tok> <tok cat="NN">site</tok> <tok cat="NN">specificity</tok></cons></cons></cons><cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">similar</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
785973507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">primary</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">receptor</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">glucocorticoid-mediated</tok> <tok cat="NN">apoptosis</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">correlates</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">transcriptional</tok></cons> <cons cat="NP"><tok cat="NN">repression</tok></cons> <cons cat="CONJP"><tok cat="RB">rather</tok> <tok cat="IN">than</tok></cons> <cons cat="NP"><tok cat="NN">activation</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">interference</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">survival</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
785974301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">HIV-1</tok> <tok cat="NN">Tat</tok></cons> <cons cat="VP"><tok cat="VBZ">potentiates</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">TNF-induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">cytotoxicity</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">altering</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">redox</tok> <tok cat="NN">state</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@16-12-2010
785974302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBZ">demonstrates</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok> <tok cat="LRB">(</tok><tok cat="NN">HIV-1</tok><tok cat="RRB">)</tok> <tok cat="NN">Tat</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBZ">amplifies</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNF</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cytokine</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">replication</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@37@@oe@16-12-2010
785974303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">HeLa</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">stably</tok></cons> <tok cat="VBN">transfected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">tat</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">HeLa-tat</tok> <tok cat="NNS">cells</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Tat</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">enhanced</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">TNF-induced</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">TNF-mediated</tok> <tok cat="NN">cytotoxicity</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
785974304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">similar</tok> <tok cat="NN">potentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">TNF</tok></cons> <tok cat="NNS">effects</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HeLa</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">soluble</tok> <cons cat="NP"><tok cat="NN">Tat</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
785974305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="JJ">TNF-mediated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">cytotoxicity</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">intracellular</tok> <tok cat="NN">formation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">reactive</tok> <tok cat="NN">oxygen</tok> <tok cat="NNS">intermediates</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
785974306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="JJ">Tat-mediated</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">redox</tok> <tok cat="NN">state</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok><cons cat="NP" ref="i9" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
785974307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HeLa</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="NP" role="SBJ"><tok cat="NN">HIV-1</tok> <tok cat="NN">Tat</tok></cons> <cons cat="VP"><tok cat="VBD">suppressed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Mn-dependent</tok> <tok cat="NN">superoxide</tok> <tok cat="NN">dismutase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Mn-SOD</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">mitochondrial</tok> <tok cat="NN">enzyme</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="NN">part</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">defense</tok> <tok cat="NN">system</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="JJ">oxidative</tok> <tok cat="NN">stress</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
785974308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i11" role="SBJ"><cons cat="NP"><tok cat="NN">Mn-SOD</tok></cons> <tok cat="NN">RNA</tok> <tok cat="NN">protein</tok> <cons cat="NP"><tok cat="NNS">levels</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">activity</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">markedly</tok></cons> <cons cat="VP"><tok cat="VBN">reduced</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Tat</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
785974309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="VBN">Decreased</tok> <cons cat="NP"><tok cat="NN">Mn-SOD</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="VBN">decreased</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">glutathione</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJR">lower</tok> <tok cat="NN">ratio</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="VBN">reduced</tok></cons><tok cat="COLON">:</tok><cons cat="ADJP"><tok cat="VBN">oxidized</tok></cons></cons> <tok cat="NN">glutathione</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
785974310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" id="i13"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="VBN">truncated</tok> <cons cat="NP"><tok cat="NN">Tat</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">Tat1-72</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" ref="i13" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">transactivate</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LTR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="ADVP"><tok cat="RB">no</tok> <tok cat="RBR">longer</tok></cons> <cons cat="VP"><tok cat="VBD">affected</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">Mn-SOD</tok></cons> <tok cat="NN">expression</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">redox</tok> <tok cat="NN">state</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">TNF-mediated</tok> <tok cat="NN">cytotoxicity</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
785974311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i16" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">C-terminal</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">Tat</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i16" null="NONE"></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">suppress</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Mn-SOD</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">pro-oxidative</tok> <tok cat="NNS">conditions</tok></cons> <cons cat="VP"><tok cat="VBN">reflected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">drop</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">reduced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glutathione</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GSH</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GSH</tok></cons><tok cat="COLON">:</tok><cons cat="NP"><cons cat="NP"><tok cat="VBN">oxidized</tok> <tok cat="NN">GSH</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GSSG</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="NN">ratio</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
785974312@GENIA Treebank@formal@@1@S@<cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">ABSTRACT</tok></cons> <cons cat="VP"><tok cat="VBN">TRUNCATED</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">AT</tok> <cons cat="NP"><tok cat="CD">250</tok> <tok cat="NNS">WORDS</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons>@@@@1@7@@oe@16-12-2010
786215701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Runt</tok> <tok cat="JJ">domain-encoding</tok> <tok cat="NN">PEBP2</tok> <tok cat="NN">alpha</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">thymic</tok> <tok cat="NN">development</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
786215702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">PEBP2</tok> <tok cat="NN">alpha</tok> <tok cat="NN">A</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">PEBP2</tok> <tok cat="NN">alpha</tok> <tok cat="NN">B</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">genes</tok></cons></cons> <cons cat="VP"><tok cat="VBP">encode</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NN">subunit</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">murine</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PEBP2</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" id="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">T-cell-specific</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">regulator</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@30@@oe@16-12-2010
786215703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="CD">two</tok> <tok cat="JJ">related</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBP">share</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">evolutionarily</tok> <tok cat="VBN">conserved</tok></cons> <tok cat="NN">region</tok></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Runt</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
786215704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PEBP2</tok> <tok cat="NN">alpha</tok> <tok cat="NN">B</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">murine</tok> <tok cat="NN">counterpart</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">AML1</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">breakpoints</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">8;21</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">3;21</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">chromosome</tok> <tok cat="NNS">translocations</tok></cons></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">leukemia</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
786215705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Northern</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RNA</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">blots</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">various</tok> <tok cat="JJ">adult</tok> <tok cat="NN">mouse</tok> <tok cat="NNS">tissues</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="RBS">most</tok> <tok cat="JJ">prominent</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">thymus</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
786215706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NNS">transcripts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PEBP2</tok> <tok cat="NN">alpha</tok> <tok cat="NN">A</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">mouse</tok> <tok cat="NN">AML1/PEBP2</tok> <tok cat="NN">alpha</tok> <tok cat="NN">B</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">thymuses</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">day</tok> <tok cat="CD">16</tok> <tok cat="NNS">embryos</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">newborns</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">4-week-old</tok></cons> <cons cat="ADJP"><tok cat="JJ">adult</tok></cons></cons> <tok cat="NNS">mice</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">situ</tok></cons> <tok cat="NN">hybridization</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
786215707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">persisted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">lymph</tok> <tok cat="NNS">nodes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="NNS">mice</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
786215708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">transcripts</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="PDT">all</tok> <tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">CD4-</tok> <tok cat="JJ">CD8-</tok><cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">CD4+</tok> <tok cat="JJ">CD8+</tok><cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">CD4+</tok> <tok cat="JJ">CD8-</tok><cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">CD4-</tok> <tok cat="JJ">CD8+</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NN">cell</tok> <tok cat="NNS">populations</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
786215709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBD">indicated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="DT">both</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">throughout</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">development</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">supporting</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">notion</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PEBP2</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">T-cell-specific</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
786215710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="NNS">Transcripts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">mouse</tok> <tok cat="NN">AML1/PEBP2</tok> <tok cat="NN">alpha</tok> <tok cat="NN">B</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">day</tok> <tok cat="CD">12</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">fetal</tok></cons> <cons cat="ADJP"><tok cat="JJ">hematopoietic</tok></cons></cons> <tok cat="NN">liver</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">bone</tok> <tok cat="NN">marrow</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">newborn</tok> <tok cat="NNS">mice</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
786215711@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">implication</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">mouse</tok> <cons cat="NP"><tok cat="NN">AML1/PEBP2</tok> <tok cat="NN">alpha</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NNS">cells</tok></cons> <cons cat="ADJP"><tok cat="JJ">other</tok> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">lineage</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">discussed</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">relation</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NN">leukemogenesis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
786216801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">cell-type-specific</tok> <cons cat="NP"><tok cat="NN">interleukin-2</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha-chain</tok></cons> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">potential</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">physical</tok> <tok cat="NNS">interactions</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Elf-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">HMG-I(Y)</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">family</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@25@@oe@16-12-2010
786216802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="CD">2</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha-chain</tok> <tok cat="LRB">(</tok><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok><tok cat="RRB">)</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP" syn="COOD"><cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">potently</tok></cons></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">mitogenic</tok> <tok cat="NNS">stimuli</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@16-12-2010
786216803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Previously</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">inducible</tok> <tok cat="NN">enhancer</tok></cons></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="NNS">nucleotides</tok> <tok cat="CD">-299</tok> <tok cat="CC">and</tok> <tok cat="CD">-228</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CArG</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">motifs</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">identified</tok><cons cat="NP" ref="i6" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
786216804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">characterization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">second</tok> <cons cat="NP"><tok cat="JJ">essential</tok> <tok cat="JJ">positive</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">element</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">nucleotides</tok> <tok cat="CD">-137</tok> <tok cat="CC">and</tok> <tok cat="CD">-64</tok></cons></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Elf-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HMG-I(Y)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
786216805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">element</tok></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="JJ">maximal</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">lymphoid</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">paralleling</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">type</tok> <tok cat="NN">specificity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Elf-1</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
786216806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">Transcription</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i9" null="NONE"></cons></cons><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i10"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" syn="COOD" id="i11" role="SBJ"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Elf-1</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HMG-I(Y)</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">binding</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">mutated</tok><cons cat="NP" ref="i11" null="NONE"></cons><cons cat="ADVP" ref="i10" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
786216807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Coexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">proteins</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">activated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="CD">-137</tok> <tok cat="TO">to</tok> <tok cat="CD">-64</tok> <tok cat="NN">element</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">COS-7</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
786216808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Elf-1</tok></cons> <cons cat="ADVP"><tok cat="RB">physically</tok></cons> <cons cat="VP"><tok cat="VBD">associated</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">HMG-I</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="NP"><tok cat="NN">p50</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Rel</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">protein-protein</tok> <tok cat="NNS">interactions</tok></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="ADVP"><tok cat="RB">functionally</tok></cons> <cons cat="VP"><tok cat="VB">coordinate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">actions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">upstream</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">downstream</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="JJ">positive</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">elements</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
786216809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NN">report</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">physical</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">Ets</tok> <tok cat="NN">family</tok> <tok cat="NN">member</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">family</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
786216810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">significant</tok></cons> <cons cat="ADJP"><tok cat="JJ">new</tok></cons></cons> <tok cat="NNS">insights</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">protein-protein</tok></cons> <cons cat="NP" ref="i26" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">protein-DNA</tok> <cons cat="NP" ref="i26" null="RNR"></cons></cons><cons cat="NP" id="i26"><tok cat="NNS">interactions</tok></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i15"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i15" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">regulate</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">cell-type-specific</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">inducible</tok></cons></cons> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok></cons> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">implications</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">Elf-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">family</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
786407201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">Effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@16-12-2010
786407202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">Glucocorticoids</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">inflammatory</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">immune</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">responses</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="PP" adv="TMP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">modulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">DNA</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@25@@oe@16-12-2010
786407203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">activator</tok> <tok cat="NN">protein-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AP-1</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cAMP-responsive</tok> <tok cat="NN">element</tok> <tok cat="NN">binding</tok> <tok cat="NN">protein</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CREB</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">DNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">gel</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@53@@oe@16-12-2010
786407204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="NN">beta</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">myristate</tok> <tok cat="NN">acetate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PMA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="NN">treatment</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="QP"><tok cat="RB">up</tok> <tok cat="TO">to</tok> <tok cat="CD">200</tok></cons><tok cat="NN">%</tok></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">decreased</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CREB</tok></cons> <tok cat="NN">binding</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">38</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">over</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">60-min</tok> <tok cat="NN">time</tok> <tok cat="NN">course</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
786407205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Dexamethasone</tok></cons> <cons cat="VP"><tok cat="VBD">produced</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">rapid</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">sustained</tok></cons></cons> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">response</tok> <tok cat="NN">element</tok></cons> <tok cat="NN">binding</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">concomitant</tok> <cons cat="ADJP"><cons cat="QP"><tok cat="CD">40-50</tok></cons><tok cat="NN">%</tok></cons> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">AP-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CREB</tok></cons></cons> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" id="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="JJ">combined</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">dexamethasone</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">cytokine</tok></cons></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">PMA</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NN">treatment</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
786407206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="JJ">latter</tok> <tok cat="NNS">effects</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">due</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">increases</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">localization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GR</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="RB">not</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">reduced</tok> <tok cat="NNS">amounts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">other</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
786407207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">GR</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">interacts</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">cytokine-stimulated</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">process</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">cross</tok> <tok cat="NN">coupling</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
786407208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">important</tok></cons> <cons cat="ADJP"><tok cat="JJ">molecular</tok></cons></cons> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">steroid</tok> <tok cat="NN">action</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
786513001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Isolation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">cDNA</tok> <tok cat="NNS">clones</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="CD">42</tok> <tok cat="JJ">different</tok> <cons cat="NP"><tok cat="JJ">Kruppel-related</tok> <tok cat="NN">zinc</tok> <tok cat="NN">finger</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">monoblast</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">U-937</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@16-12-2010
786513002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S" syn="COOD" func="ADV"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">study</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">complexity</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">structural</tok> <tok cat="NNS">characteristics</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">zinc</tok> <tok cat="NN">finger</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">hematopoiesis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">isolate</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">novel</tok> <tok cat="NNS">regulators</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">blood</tok> <tok cat="NN">cell</tok> <tok cat="NN">development</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">degenerate</tok> <tok cat="NN">oligonucleotide</tok> <tok cat="NN">probe</tok></cons> <cons cat="ADJP"><tok cat="JJ">specific</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">consensus</tok> <tok cat="NN">zinc</tok> <tok cat="NN">finger</tok> <tok cat="NN">peptide</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i5" null="NONE"></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">isolate</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">63</tok> <cons cat="NP"><tok cat="NN">cDNA</tok> <tok cat="NNS">clones</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">Kruppel-related</tok> <tok cat="NN">zinc</tok> <tok cat="NN">finger</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">monoblast</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">U-937</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@57@@oe@16-12-2010
786513003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="JJ">extensive</tok> <tok cat="NN">nucleotide</tok> <tok cat="NN">sequence</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Northern</tok> <tok cat="NN">blot</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NN">analysis</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NN">cDNA</tok> <tok cat="NNS">clones</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">originate</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="RB">approximately</tok> <tok cat="CD">42</tok></cons> <tok cat="JJ">different</tok> <tok cat="NNS">genes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HZF</tok> <tok cat="CD">1-42</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="IN">of</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="RB">only</tok> <tok cat="CD">8</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">described</tok><cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
786513004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Northern</tok> <tok cat="NN">blot</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">majority</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="JJ">comparable</tok> <tok cat="NNS">levels</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">U-937</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HeLa</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
786513005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">large</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">individual</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBN">represented</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">among</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">63</tok> <tok cat="NNS">clones</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <cons cat="ADJP"><tok cat="JJ">apparent</tok> <tok cat="JJ">non-cell-type-specific</tok></cons> <tok cat="NN">expression</tok></cons></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">majority</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">Kruppel-related</tok> <tok cat="NN">zinc</tok> <tok cat="NN">finger</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">likely</tok> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJS">most</tok> <tok cat="JJ">human</tok></cons> <tok cat="NNS">tissues</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
786513006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">some</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">displayed</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">restricted</tok> <tok cat="NN">expression</tok> <tok cat="NN">pattern</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBP">represent</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">potential</tok> <tok cat="NNS">regulators</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="NN">monocyte</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">proliferation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
786513007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">Detailed</tok></cons> <cons cat="ADJP"><tok cat="JJ">structural</tok></cons></cons> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="CD">12</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cDNAs</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HZF</tok> <tok cat="CD">1-10</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">partial</tok> <tok cat="NN">characterization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HZF</tok> <tok cat="CD">11-42</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">common</tok> <tok cat="NN">feature</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">Kruppel-related</tok> <tok cat="NN">zinc</tok> <tok cat="NN">finger</tok> <tok cat="NNS">proteins</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">tandem</tok> <tok cat="NNS">arrays</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">zinc</tok> <tok cat="NNS">fingers</tok></cons></cons> <cons cat="VP"><tok cat="VBG">ranging</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">number</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="CD">3</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="QP"><tok cat="IN">over</tok> <tok cat="CD">20</tok></cons></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i11"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">preferentially</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">carboxy-terminal</tok> <tok cat="NNS">regions</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@59@@oe@16-12-2010
786513008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i14" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">several</tok> <tok cat="JJ">novel</tok></cons> <cons cat="NP"><tok cat="JJ">KRAB-containing</tok> <tok cat="NN">zinc</tok> <tok cat="NN">finger</tok> <tok cat="NNS">genes</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <cons cat="NP"><tok cat="VBN">conserved</tok> <tok cat="NN">sequence</tok> <tok cat="NN">element</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">identified</tok><cons cat="NP" ref="i14" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
786903801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Calcium/calmodulin-dependent</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="CD">II</tok></cons> <cons cat="VP"><tok cat="VBZ">downregulates</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">calcineurin</tok> <cons cat="ADJP" null="QSTN"></cons><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="JJ">C-mediated</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">pathways</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@16-12-2010
786903802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Engagement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">receptor</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">antigen</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="NN">phospholipase</tok> <tok cat="NN">C</tok></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="JJ">free</tok> <tok cat="NN">calcium</tok> <tok cat="NN">concentration</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">[Ca2+]i</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PKC</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@33@@oe@16-12-2010
786903803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="VBN">Increased</tok> <tok cat="NN">[Ca2+]i</tok></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Ca2+/calmodulin-dependent</tok> <tok cat="NNS">kinases</tok></cons> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">multifunctional</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Ca2+/calmodulin-dependent</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="CD">II</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CaM-K</tok> <tok cat="CD">II</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">calcineurin</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">type</tok> <tok cat="NN">2B</tok> <tok cat="NN">protein</tok> <tok cat="NN">phosphatase</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
786903804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Recent</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP"><tok cat="NN">calcineurin</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">key</tok> <tok cat="NN">enzyme</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="CD">-2</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-4</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">promoter</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
786903805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CaM-K</tok> <tok cat="CD">II</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">remains</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unknown</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
786903806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">mutants</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NNS">kinases</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">phosphatases</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">gamma</tok> <tok cat="NN">B*CaM-K</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">delta</tok> <tok cat="NN">CaM-AI</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">explore</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">relative</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">transcription</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NNS">interactions</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">PKC-dependent</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">systems</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
786903807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP" syn="COOD" id="i6"><cons cat="NP"><tok cat="NN">gamma</tok> <tok cat="NN">B*CaM-K</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">delta</tok> <tok cat="NN">CaM-AI</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">exhibit</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">constitutive</tok> <tok cat="JJ">Ca(2+)-independent</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">cotransfected</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="UCP" syn="COOD"><cons cat="ADVP"><tok cat="RB">alone</tok></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">combination</tok></cons></cons></cons><tok cat="RRB">)</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">plasmid</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">intact</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons> <cons cat="VP"><tok cat="VBG">driving</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">chloramphenicol</tok> <tok cat="NN">acetyltransferase</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
786903808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Cotransfection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">gamma</tok> <tok cat="NN">B*CaM-K</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok> <tok cat="NN">construct</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">downregulated</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ionomycin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">myristate</tok> <tok cat="NN">acetate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PMA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
786903809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CaM-K</tok> <tok cat="CD">II</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">decreased</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="PRPP">its</tok> <cons cat="NP"><tok cat="NN">AP-1</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NN">transactivating</tok> <tok cat="NNS">pathways</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
786903810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Under</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NNS">conditions</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">delta</tok> <tok cat="NN">CaM-AI</tok></cons> <cons cat="VP"><tok cat="VBD">superinduced</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="ADJP"><tok cat="RB">approximately</tok> <tok cat="JJ">twofold</tok></cons> <tok cat="NN">increase</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
786903811@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i10"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="DT">both</tok> <tok cat="NNS">mutants</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">combination</tok></cons></cons><cons cat="ADVP" ref="i10" null="T"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">gamma</tok> <tok cat="NN">B*CaM-K</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">delta</tok> <tok cat="NN">CaM-AI</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
786903812@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="JJ">Similar</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" ref="i12" null="NONE"></cons></cons><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i13"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">construct</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-4</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">used</tok><cons cat="NP" ref="i14" null="NONE"></cons><cons cat="ADVP" ref="i13" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
786903813@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">gamma</tok> <tok cat="NN">B*CaM-K</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">downregulated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">transfection</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">constitutively</tok> <tok cat="JJ">active</tok></cons> <tok cat="NN">mutant</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PKC</tok></cons></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">PMA</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
786903814@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">CaM-K</tok> <tok cat="CD">II</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">exert</tok> <cons cat="NP"><tok cat="JJ">negative</tok> <tok cat="NNS">influences</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">preliminary</tok> <tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">negative</tok> <tok cat="NN">cross-talk</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">calcineurin-</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">PKC-</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="JJ">dependent</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">systems</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
787387601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Deleted</tok> <cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="CD">20</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">patient</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">Alagille</tok> <tok cat="NN">syndrome</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cell</tok> <tok cat="NN">hybrid</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="NN">leucine</tok> <tok cat="NN">transport</tok> <tok cat="NN">selection</tok></cons></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">study</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">three</tok> <tok cat="NN">candidate</tok> <tok cat="NNS">genes</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@25@@oe@16-12-2010
787387602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Alagille</tok> <tok cat="NN">syndrome</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AGS</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">well-defined</tok> <tok cat="JJ">genetic</tok> <tok cat="NN">entity</tok></cons> <cons cat="VP"><tok cat="VBN">assigned</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">short</tok> <tok cat="NN">arm</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Chromosome</tok> <tok cat="LRB">(</tok><tok cat="NN">Chr</tok><tok cat="RRB">)</tok> <tok cat="CD">20</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">series</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">observations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AGS</tok> <tok cat="NNS">patients</tok></cons></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">microdeletions</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@36@@oe@16-12-2010
787387603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">fusing</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">lymphoblastoid</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">AGS</tok> <tok cat="NN">patient</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">exhibited</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">microdeletion</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">short</tok> <tok cat="NN">arm</tok> <tok cat="IN">of</tok> <tok cat="NN">Chr</tok> <tok cat="CD">20</tok></cons></cons> <cons cat="VP"><tok cat="NN">encompassing</tok> <cons cat="NP"><tok cat="NNS">bands</tok> <cons cat="NP"><tok cat="NN">p11.23</tok> <tok cat="TO">to</tok> <tok cat="VB">p12.3</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">rodent</tok> <tok cat="JJ">thermosensitive</tok> <tok cat="JJ">mutant</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CHOtsH1-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">deficient</tok> <cons cat="NP"><tok cat="JJ">in-leucyl-tRNA</tok> <tok cat="NN">synthetase</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">isolated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">somatic</tok> <tok cat="NN">cell</tok> <tok cat="NN">hybrid</tok></cons></cons> <cons cat="VP"><tok cat="VBG">segregating</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">deleted</tok> <tok cat="JJ">human</tok> <cons cat="NP"><tok cat="NN">Chr</tok> <tok cat="CD">20</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@49@@oe@16-12-2010
787387604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">This</tok> <tok cat="NN">hybrid</tok> <tok cat="NN">clone</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">designated</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NN">NR2</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">characterized</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NNS">methods</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PCR</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">eight</tok> <tok cat="NNS">pairs</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">oligonucleotides</tok> <cons cat="NP" ref="i32" null="ICH"></cons></cons></cons><cons cat="VP"><tok cat="VBN">mapped</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">Chr</tok> <tok cat="CD">20</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD" id="i32"><cons cat="NP"><tok cat="NN">D20S5</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">D20S41</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">D20S42</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">D20S56</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">D20S57</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">D20S58</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">adenosine</tok> <tok cat="NN">deaminase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ADA</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Prion</tok> <tok cat="NN">protein</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PRIP</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Restriction</tok> <tok cat="NN">Fragment</tok> <tok cat="NN">Length</tok> <tok cat="NN">Polymorphism</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RFLP</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">analyses</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">four</tok> <cons cat="NP"><tok cat="JJ">genomic</tok> <tok cat="JJ">anonymous</tok> <tok cat="NNS">probes</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">D20S5</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">cD3H12</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">D20S17</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">D20S18</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">fluorescent</tok> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">situ</tok></cons> <tok cat="NN">hybridization</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">FISH</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">total</tok> <tok cat="JJ">human</tok> <tok cat="NN">DNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">D20Z1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">sequence</tok></cons> <cons cat="ADJP"><tok cat="JJ">specific</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">Chr</tok> <tok cat="CD">20</tok> <tok cat="NN">centromere</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="NNS">probes</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@102@@oe@16-12-2010
787387605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">NR2</tok> <tok cat="NN">hybrid</tok></cons></cons> <cons cat="VP"><tok cat="VBD">allowed</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">us</tok></cons> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">exclude</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok> <cons cat="NP"><tok cat="NN">candidate</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">AGS</tok></cons></cons><cons cat="NP" ref="i30" null="ICH"></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP"><cons cat="NP" syn="COOD" id="i30"><cons cat="NP"><cons cat="NP"><tok cat="JJ">hepatic</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="CD">3</tok> <tok cat="NN">beta</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HNF3</tok> <tok cat="NN">beta</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">paired</tok> <tok cat="NN">box</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PAX1</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cystatin</tok> <tok cat="NN">C</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CST3</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">localization</tok></cons> <cons cat="ADJP"><tok cat="JJ">outside</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">deletion</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@16-12-2010
787387606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">NR2</tok> <tok cat="NN">hybrid</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">powerful</tok> <tok cat="NN">tool</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mapping</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">new</tok> <tok cat="NNS">probes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">obtaining</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">new</tok></cons> <cons cat="ADJP"><tok cat="JJ">informative</tok></cons></cons> <tok cat="NNS">probes</tok></cons> <cons cat="ADJP"><tok cat="JJ">specific</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">deletion</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">subtractive</tok> <tok cat="NN">cloning</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
787387607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Such</tok> <tok cat="NNS">markers</tok></cons> <cons cat="VP"><tok cat="MD">will</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">useful</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">linkage</tok> <tok cat="NN">analysis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">screening</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cDNA</tok> <tok cat="NNS">libraries</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010