862779101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">Efficient</tok></cons> <cons cat="NP"><tok cat="NN">transcription</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">replication</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">simian</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">absence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappaB</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Sp1</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">binding</tok> <tok cat="NNS">elements</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@16-12-2010
862779102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="CD">Ten</tok> <tok cat="NNS">mutants</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">simian</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SIV</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="NP"><tok cat="NN">SIVmac239</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBG">bearing</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NNS">deletions</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">delta</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">substitutions</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">subst</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and/or</tok> <cons cat="NP"><tok cat="NN">Sp1</tok> <tok cat="NN">binding</tok> <tok cat="NNS">elements</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">created</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">replicative</tok> <tok cat="NNS">capacities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">mutants</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok><cons cat="NP" ref="i6" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@41@@oe@16-12-2010
862779103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">All</tok> <tok cat="NNS">mutants</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok> <cons cat="ADJP"><tok cat="RB">extensively</tok> <tok cat="VBN">mutagenized</tok></cons> <tok cat="NN">strain</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">entirely</tok></cons> <tok cat="VBG">missing</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappaB</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">four</tok> <tok cat="NN">Spl</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">binding</tok> <tok cat="NNS">elements</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBN">replicated</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">wild-type</tok> <tok cat="NNS">kinetics</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">wild-type</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NN">cell</tok> <tok cat="NNS">cultures</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">50</tok> <tok cat="TO">to</tok> <tok cat="CD">100</tok></cons><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">experiments</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
862779104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">One</tok> <tok cat="NN">group</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">mutants</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">replicated</tok> <cons cat="ADVP"><cons cat="ADVP"><tok cat="RB">very</tok> <tok cat="RB">similarly</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">SIVmac239</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">kinetics</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">yield</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">CEMxl74</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">2</tok></cons><tok cat="CC">x</tok><cons cat="NP"><tok cat="NN">NFKappaB</tok></cons></cons> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">&gt;</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">=</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">SlVmac239</tok></cons> <cons cat="ADVP"><tok cat="RB">approximately</tok></cons> <cons cat="NP"><tok cat="NN">deltaNFkappaB</tok></cons> <cons cat="ADVP"><tok cat="RB">approximately</tok></cons> <cons cat="NP"><tok cat="NN">deltaSpl234</tok></cons> <cons cat="ADVP"><tok cat="RB">approximately</tok></cons> <cons cat="NP"><tok cat="NN">substNFkappaB</tok></cons> <cons cat="ADVP"><tok cat="RB">approximately</tok></cons> <cons cat="NP"><tok cat="NN">substSpl2</tok></cons> <cons cat="ADVP"><tok cat="RB">approximately</tok></cons> <cons cat="NP"><tok cat="NN">substSp23</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">a</tok> <tok cat="JJ">second</tok> <tok cat="NN">group</tok></cons> <cons cat="VP"><tok cat="VBD">replicated</tok> <cons cat="PP"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">delayed</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">slightly</tok> <tok cat="VBN">delayed</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NN">kinetics</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">CEMxl74</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">SIVmac239</tok></cons> <cons cat="ADJP"><tok cat="JJ">&gt;</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">substSp34</tok></cons> <cons cat="ADJP"><tok cat="JJ">&gt;</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">deltaNFkappaBdeltaSpl234</tok></cons> <cons cat="ADVP"><tok cat="RB">approximately</tok></cons> <cons cat="NP"><tok cat="NN">deltaNFkappaBdeltaSp1</tok></cons> <cons cat="ADJP"><tok cat="JJ">&gt;</tok> <cons cat="NP"><tok cat="NN">substSpl234</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@62@@oe@16-12-2010
862779105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i8" role="SBJ"><cons cat="NP"><tok cat="NNS">Reversions</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">additional</tok> <tok cat="NNS">mutations</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">U3</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">R</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NNS">regions</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">proviral</tok> <tok cat="NN">DNA</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CEMxl74</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">infected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">SIVmac239</tok> <tok cat="NNS">mutants</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
862779106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="JJ">Similar</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" ref="i9" null="NONE"></cons></cons><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i7"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NNS">mutants</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SIVmacMER</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">macrophage-competent</tok> <tok cat="NN">derivative</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SIVmac239</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">tested</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NN">cell</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CEMx174</tok> <tok cat="NNS">cultures</tok></cons></cons></cons><cons cat="ADVP" ref="i7" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
862779107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">growth</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">most</tok> <tok cat="VBN">mutated</tok> <tok cat="NNS">viruses</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">suppressed</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">rhesus</tok> <tok cat="NN">monkey</tok> <tok cat="JJ">alveolar</tok> <tok cat="NNS">macrophages</tok></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">SIVmacMER</tok></cons> <cons cat="ADVP"><tok cat="RB">approximately</tok></cons> <cons cat="NP"><tok cat="NN">2xNFkappaB</tok></cons> <cons cat="ADVP"><tok cat="RB">approximately</tok></cons> <cons cat="NP"><tok cat="NN">substNFkappaB</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">&gt;</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">deltaNFkappaB</tok></cons> <cons cat="ADJP"><tok cat="JJ">&gt;</tok> <cons cat="NP"><tok cat="NN">deltaNFkappaBdeltaSpl234</tok> <cons cat="ADVP"><tok cat="RB">approximately</tok></cons> <cons cat="NP"><tok cat="NN">deltaNFkappaBdeltaSpl</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">&gt;</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">deltaSpl234</tok></cons> <cons cat="ADVP"><tok cat="RB">approximately</tok></cons> <cons cat="NP"><tok cat="NN">substSpl2</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">&gt;</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">substSp23</tok></cons> <cons cat="ADVP"><tok cat="RB">approximately</tok></cons> <cons cat="NP"><tok cat="NN">substSp34</tok></cons> <cons cat="ADVP"><tok cat="RB">approximately</tok></cons> <cons cat="NP"><tok cat="NN">substSpl234</tok></cons></cons> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">&gt;</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">=</tok></cons> <cons cat="NP"><tok cat="NN">SIVmac239</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="RRB">)</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
862779108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Sp1</tok> <tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RBS">most</tok> <tok cat="JJ">dramatic</tok></cons> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">SIVmac</tok> <tok cat="NN">replication</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">macrophage</tok> <tok cat="NNS">cultures</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
862779109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="JJ">repeat-driven</tok> <tok cat="VBN">secreted</tok></cons> <cons cat="NP"><tok cat="NN">alkaline</tok> <tok cat="NN">phosphatase</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NNS">assays</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok><cons cat="S"><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">unlike</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">SIV</tok> <tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">possesses</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">enhancer</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">just</tok> <tok cat="RB">upstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">element</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i13"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i13" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">maintains</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">significant</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">basal</tok> <tok cat="NN">transcription</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">absence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappaB</tok> <cons cat="NP" ref="i24" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Sp1</tok> <cons cat="NP" ref="i24" null="RNR"></cons></cons><cons cat="NP" id="i24"><tok cat="NNS">sites</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@53@@oe@16-12-2010
862779110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">region</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">responsive</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">Tat</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
862779111@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i14" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">SIV</tok> <tok cat="NN">TATA</tok> <tok cat="NN">box</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i14" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="JJR">stronger</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
862779112@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">surprisingly</tok> <tok cat="JJ">high</tok> <tok cat="JJ">replicative</tok></cons> <tok cat="NN">capacity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappaB</tok> <cons cat="NP" ref="i25" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Sp1</tok> <cons cat="NP" ref="i25" null="RNR"></cons></cons><cons cat="NP" id="i25"><tok cat="NN">binding</tok> <tok cat="NN">site</tok> <tok cat="NNS">mutants</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SIVmac</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">due</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">unique</tok> <tok cat="NNS">features</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">enhancer/promoter</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
862827401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Inactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IkappaBbeta</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">tax</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok></cons></cons></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potential</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">constitutive</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@16-12-2010
862827402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="VBG">resting</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">sequestered</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytoplasm</tok></cons></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">interactions</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">family</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">inhibitors</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IkappaBalpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IkappaBbeta</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@32@@oe@16-12-2010
862827403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">During</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">activation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><tok cat="NN">IkappaBalpha</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">phosphorylated</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">ubiquitinated</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">degraded</tok></cons> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">26S</tok> <tok cat="NN">proteasome</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="VP"><tok cat="VBG">permitting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">release</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">functional</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
862827404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">contrast</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">transient</tok> <tok cat="NN">pattern</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">induction</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><tok cat="NN">NF-kappaB</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Tax</tok> <tok cat="NN">transforming</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">I</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HTLV-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
862827405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Recent</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HTLV-1</tok> <cons cat="NP"><tok cat="NN">Tax</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">targets</tok> <cons cat="NP"><tok cat="NN">IkappaBalpha</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">ubiquitin-proteasome</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
862827406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRP">it</tok></cons> <cons cat="SBAR" ref="i7" null="EXP"></cons></cons><cons cat="VP"><tok cat="VBZ">remains</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unclear</tok></cons> <cons cat="SBAR" id="i7"><cons cat="WHADVP" id="i9"><tok cat="WRB">how</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">persistent</tok></cons> <cons cat="CONJP"><tok cat="RB">rather</tok> <tok cat="IN">than</tok></cons> <cons cat="ADJP"><tok cat="JJ">transient</tok></cons></cons> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <tok cat="NN">response</tok></cons><cons cat="ADVP" ref="i9" null="T"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
862827407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">report</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><tok cat="NN">evidence</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">acting</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">IkappaBalpha</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i11" role="SBJ"><tok cat="NN">Tax</tok></cons> <cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">turnover</tok></cons> <cons cat="PP"><tok cat="IN">Of</tok> <cons cat="NP"><tok cat="NN">IkappaBbeta</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">related</tok> <tok cat="NN">targeting</tok> <tok cat="NN">mechanism</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
862827408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Like</tok> <cons cat="NP"><tok cat="NN">IkappaBalpha</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i15" role="SBJ"><cons cat="NP"><tok cat="JJ">Tax-mediated</tok> <tok cat="NN">breakdown</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IkappaBbeta</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">transfected</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP" syn="COOD"><tok cat="CC">either</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">cell-permeable</tok> <tok cat="NN">proteasome</tok> <tok cat="NNS">inhibitors</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">mutation</tok></cons> <cons cat="PP"><tok cat="IN">Of</tok> <cons cat="NP"><tok cat="NN">IkappaBbeta</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NN">serine</tok> <tok cat="NNS">residues</tok></cons> <cons cat="ADJP"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <cons cat="NP"><tok cat="JJ">N-terminal</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
862827409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Despite</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">dual</tok> <tok cat="NN">specificity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HTLV-1</tok> <cons cat="NP"><tok cat="NN">Tax</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IkappaBalpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IkappaBbeta</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">protein</tok> <tok cat="NN">level</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">Tax</tok></cons> <cons cat="ADVP"><tok cat="RB">selectively</tok></cons> <cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">NF-kappaB-directed</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IkappaBalpha</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
862827410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Consequently</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i16" role="SBJ"><cons cat="NP"><tok cat="NN">IkappaBbeta</tok></cons> <tok cat="NN">protein</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">chronically</tok></cons> <cons cat="VP"><tok cat="VBN">downregulated</tok> <cons cat="NP" ref="i16" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">HTLV-1-infected</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
862827411@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IkappaBbeta</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potential</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">constitutive</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">Tax-expressing</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
862828401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Genetic</tok> <tok cat="NN">characterization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">Binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Tax</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">Tax-responsive</tok> <tok cat="NN">element</tok> <tok cat="CD">1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">cyclic</tok> <tok cat="JJ">AMP-responsive</tok> <tok cat="NNS">members</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CREB/ATF</tok> <tok cat="NN">family</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@36@@oe@16-12-2010
862828402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">achieve</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJR">better</tok> <tok cat="NN">understanding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">Tax</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Tax-responsive</tok> <tok cat="NN">element</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TRE-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">developed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">genetic</tok> <tok cat="NN">approach</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">Saccharomyces</tok> <tok cat="NN">cerevisiae</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@35@@oe@16-12-2010
862828403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">constructed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">yeast</tok> <tok cat="NN">reporter</tok> <tok cat="NN">strain</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">lacZ</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CYC1</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok> <tok cat="NNS">copies</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TRE-1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
862828404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cyclic</tok> <tok cat="NN">AMP</tok> <tok cat="NN">response</tok> <tok cat="NN">element-binding</tok> <tok cat="NN">protein</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CREB</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CREB</tok></cons> <cons cat="VP"><tok cat="VBN">fused</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GAL4</tok> <tok cat="NN">activation</tok> <tok cat="NN">domain</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GAD</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">strain</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">modify</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
862828405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Tax</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">inactive</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@6@@oe@16-12-2010
862828406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">coexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Tax</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CREB</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
862828407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">effect</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJR">stronger</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GAD-CREB</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
862828408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <cons cat="NP"><tok cat="NN">CREB</tok> <tok cat="NNS">mutants</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">genetic</tok> <tok cat="NN">system</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">indicated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">C-terminal</tok> <tok cat="CD">92</tok> <tok cat="NN">amino</tok> <tok cat="NN">acid</tok> <tok cat="NNS">residues</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">include</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">basic</tok> <tok cat="NN">domain</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">leucine</tok> <tok cat="NN">zipper</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">necessary</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">sufficient</tok></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">mediate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">Tax</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
862828409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">identify</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">TRE-1</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">Tax-dependent</tok> <tok cat="NN">manner</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">strain</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i8" null="NONE"></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">screen</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">library</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">cDNAs</tok></cons> <cons cat="VP"><tok cat="VBN">fused</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">GAD</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
862828410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="PP"><tok cat="IN">Of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">five</tok> <tok cat="JJ">positive</tok> <tok cat="NNS">clones</tok></cons> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="QP"><tok cat="CD">0.75</tok> <tok cat="CC">x</tok> <tok cat="CD">10(6)</tok></cons> <tok cat="NN">yeast</tok> <tok cat="NNS">colonies</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="CD">four</tok> <cons cat="NP" ref="i30" null="ICH"></cons></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">CREB/activating</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="LRB">(</tok><tok cat="NN">ATF</tok><tok cat="RRB">)</tok> <tok cat="NN">family</tok></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD" id="i30"><cons cat="NP"><tok cat="NN">CREB</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">isoforms</tok> <tok cat="IN">of</tok> <tok cat="DT">the</tok> <tok cat="JJ">cyclic</tok> <tok cat="JJ">AMP-responsive</tok> <tok cat="NN">element</tok> <tok cat="NN">modulator</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CREM</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">ATF-1</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
862828411@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Interestingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><tok cat="DT">these</tok> <tok cat="CD">three</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">phosphorylated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">A</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="VP"><tok cat="VB">form</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">particular</tok> <tok cat="NN">subgroup</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CREB/ATF</tok> <tok cat="NN">family</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
862828412@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ATF-2</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="FW">S.</tok> <tok cat="FW">cerevisiae</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="NN">TRE-1</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Tax</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
862828413@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBZ">shows</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">eukaryotic</tok> <tok cat="NN">nucleus</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">Tax</tok></cons> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="VP"><tok cat="VBZ">interacts</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">basic</tok> <tok cat="JJ">domain-leucine</tok> <tok cat="NN">zipper</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ATF-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CREB</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CREM</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
862828414@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">fifth</tok> <tok cat="NN">clone</tok></cons> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">screening</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">corresponded</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Ku</tok> <tok cat="NN">autoantigen</tok> <tok cat="NN">p70</tok> <tok cat="NN">subunit</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
862828415@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i10"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">fused</tok> <cons cat="NP" ref="i16" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">GAD</tok></cons></cons><cons cat="ADVP" ref="i10" null="T"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i16" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">C-terminal</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Ku</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i16" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="NN">TRE-1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">Tax</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
862829501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">EGR1</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">stimulus-dependent</tok> <cons cat="NP"><tok cat="NN">CD44</tok></cons> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
862829502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">immediate-early</tok> <tok cat="NN">gene</tok></cons> <cons cat="NP"><tok cat="NN">egr-1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">encodes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EGR1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">links</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">antigen</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">BCR</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">signals</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">downstream</tok> <tok cat="NN">activation</tok> <tok cat="NNS">events</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">previously</tok> <tok cat="JJ">unidentified</tok></cons> <cons cat="NP"><tok cat="NN">target</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@33@@oe@16-12-2010
862829503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">identify</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">gene</tok></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">lymphocyte</tok> <tok cat="NN">homing</tok> <tok cat="CC">and</tok> <tok cat="NN">migration</tok> <tok cat="NN">protein</tok></cons> <cons cat="NP"><tok cat="NN">CD44</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">target</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">EGR1</tok></cons> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
862829504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="JJ">BCR-induced</tok> <tok cat="NNS">increases</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CD44</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="NP"><tok cat="NN">expression</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">levels</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">occur</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">EGR1-expressing</tok></cons> <cons cat="NP" null="QSTN"></cons><cons cat="PP" ref="i30" null="RNR"></cons></cons></cons><tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP" null="QSTN"></cons><tok cat="JJ">nonexpressing</tok> <tok cat="NNS">subclones</tok></cons> <cons cat="PP" ref="i30" null="RNR"></cons></cons><cons cat="PP" id="i30"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">line</tok></cons> <cons cat="NP"><tok cat="NN">WEHI-231</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
862829505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NNS">Kinetics</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">egr-1</tok></cons> <tok cat="NN">transcription</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">appearance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <cons cat="NP"><tok cat="NN">EGR1</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">precede</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD44</tok></cons> <tok cat="NN">induction</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">occur</tok> <cons cat="PP" adv="TMP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">30</tok> <tok cat="NN">min</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">EGR1-expressing</tok> <tok cat="NN">subclone</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
862829506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">A</tok> <tok cat="JJ">single</tok> <cons cat="NP"><tok cat="NN">EGR1</tok></cons> <tok cat="NN">binding</tok> <tok cat="NN">motif</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">bp</tok> <tok cat="CD">-301</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">CD44</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
862829507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Cotransfection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">CD44</tok> <tok cat="JJ">promoter-chloramphenicol</tok> <tok cat="NN">acetyltransferase</tok> <tok cat="NN">reporter</tok> <tok cat="NN">construct</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">egr-1</tok> <tok cat="NN">expression</tok> <tok cat="NN">vector</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="CD">6.5-</tok> <tok cat="TO">to</tok> <tok cat="JJ">8.5-fold</tok></cons> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="ADJP"><tok cat="JJ">relative</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">empty</tok> <tok cat="NN">expression</tok> <tok cat="NN">vector</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
862829508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">EGR1</tok> <tok cat="NN">binding</tok> <tok cat="NN">motif</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">necessary</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">stimulus-induced</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">CD44</tok></cons> <cons cat="NP"><tok cat="JJ">promoter-chloramphenicol</tok> <tok cat="NN">acetyltransferase</tok> <tok cat="NN">reporter</tok> <tok cat="NN">construct</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">nontransformed</tok> <tok cat="NN">B</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">EGR1</tok> <tok cat="NN">expression</tok> <tok cat="NN">vector</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">line</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
862829509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">identify</tok> <cons cat="NP"><tok cat="NN">EGR1</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">intermediary</tok></cons> <cons cat="VP"><tok cat="VBG">linking</tok> <cons cat="NP"><tok cat="JJ">BCR-derived</tok> <tok cat="NNS">signals</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD44</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
862829510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">relevance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="JJ">molecular</tok> <tok cat="NNS">events</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">BCR</tok></cons> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">antigen-stimulated</tok></cons> <cons cat="ADJP"><tok cat="JJ">B-cell-mediated</tok></cons></cons> <tok cat="JJ">immune</tok> <tok cat="NNS">responses</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">discussed</tok><cons cat="NP" ref="i9" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
862830601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Identification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">inducible</tok> <tok cat="NN">regulator</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-myb</tok></cons> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T-cell</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@12@@oe@16-12-2010
862830602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="VBG">Resting</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">very</tok> <tok cat="JJ">low</tok></cons> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-Myb</tok> <tok cat="NN">protein</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@11@@oe@16-12-2010
862830603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">During</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">activation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">c-myb</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i5" null="NONE"></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">much</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">expression</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">level</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
862830604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">c-myb</tok> <tok cat="JJ">5'</tok> <tok cat="JJ">flanking</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-myb</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
862830605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">In</tok> <tok cat="FW">vivo</tok></cons> <tok cat="NN">footprinting</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">ligation-mediated</tok> <tok cat="NN">PCR</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">performed</tok> <cons cat="NP" ref="i6" null="NONE"></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">correlate</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <tok cat="NN">protein</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
862830606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">A</tok> <tok cat="NN">protein</tok> <tok cat="NN">footprint</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">visible</tok> <cons cat="PP"><tok cat="IN">over</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">c-myb</tok> <tok cat="JJ">5'</tok> <tok cat="JJ">flanking</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">unactivated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
862830607@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">An</tok> <tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EMSA</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">extract</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">oligonucleotide</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="VBG">binding</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="NP"><cons cat="NP" id="i7"><tok cat="DT">a</tok> <tok cat="JJ">new</tok> <cons cat="NP"><tok cat="JJ">protein-DNA</tok> <tok cat="NN">complex</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">referred</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" ref="i7" null="NONE"></cons></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">CMAT</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">c-myb</tok> <tok cat="IN">in</tok> <tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">complex</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">unactivated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@49@@oe@16-12-2010
862830608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Because</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="VBG">binding</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">some</tok> <tok cat="NN">sequence</tok> <tok cat="NN">similarity</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="IN">of</tok> <tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok> <tok cat="LRB">(</tok><tok cat="NN">NFAT</tok><tok cat="RRB">)</tok> <tok cat="NN">binding</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">compared</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">kinetics</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="VBG">binding</tok> <tok cat="NNS">complexes</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NNS">masses</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
862830609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Studies</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">kinetics</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">induction</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NFAT</tok> <tok cat="NN">EMSA</tok> <tok cat="NN">binding</tok> <tok cat="NN">complex</tok></cons></cons> <cons cat="VP"><tok cat="VBD">appeared</tok> <cons cat="ADVP" adv="TMP"><cons cat="ADVP"><tok cat="RBR">earlier</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">than</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CMAT</tok> <tok cat="NN">complex</tok></cons></cons><cons cat="VP" null="QSTN"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
862830610@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">NFAT</tok></cons> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBD">migrated</tok> <cons cat="ADVP"><cons cat="ADVP"><tok cat="RBR">more</tok> <tok cat="RB">slowly</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">sodium</tok> <tok cat="NN">dodecyl</tok> <tok cat="JJ">sulfate-polyacrylamide</tok> <tok cat="NN">gel</tok></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">than</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CMAT</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok><cons cat="VP" null="QSTN"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
862830611@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">antibody</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="NN">NFAT</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">cross-react</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CMAT</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
862830612@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">appearance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CMAT</tok> <tok cat="NN">binding</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">cyclosporin</tok> <tok cat="NN">A</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">rapamycin</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
862830613@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">CMAT</tok></cons> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">novel</tok></cons> <cons cat="ADJP"><tok cat="JJ">inducible</tok></cons></cons> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-myb</tok></cons> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
863180901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="NN">cyclosporin</tok> <tok cat="JJ">A-resistant</tok></cons> <tok cat="NN">pathway</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
863180902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">action</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immunosuppressive</tok> <tok cat="NN">drug</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cyclosporin</tok> <tok cat="NN">A</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CsA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">inactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">Ca2+/calmodulin-dependent</tok> <tok cat="NN">serine-threonine</tok> <tok cat="NN">phosphatase</tok> <tok cat="NN">calcineurin</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">drug-immunophilin</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
863180903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i49" role="SBJ"><tok cat="JJ">Inactive</tok> <tok cat="NN">calcineurin</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unable</tok> <cons cat="S"><cons cat="NP" ref="i49" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NFAT</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="CD">2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-2</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
863180904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i50" role="SBJ"><cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">CsA-treated</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">dramatically</tok></cons> <tok cat="VBN">reduced</tok><cons cat="NP" ref="i50" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
863180905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i51" role="SBJ"><tok cat="NN">NFAT</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">activated</tok><cons cat="NP" ref="i51" null="NONE"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i52" role="SBJ"><cons cat="NP"><tok cat="JJ">significant</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" ref="i52" null="NONE"></cons></cons></cons></cons></cons></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="JJ">CsA-resistant</tok> <tok cat="JJ">CD28-signaling</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
863180906@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">transfection</tok> <tok cat="NNS">assays</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i53" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="JJ">multicopy</tok> <tok cat="NN">NFAT-</tok> <cons cat="NP" ref="i54" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter-beta-galactosidase</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok> <cons cat="NP" ref="i54" null="RNR"></cons></cons></cons><cons cat="NP" id="i54"><tok cat="NNS">constructs</tok></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i53" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">phorbol</tok> <tok cat="NN">12-myristate</tok> <tok cat="NN">13-acetate</tok> <tok cat="LRB">(</tok><tok cat="NN">PMA</tok><tok cat="RRB">)</tok><tok cat="NN">/alpha-CD28</tok> <tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">resistant</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">CsA</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
863180907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">CsA-resistant</tok> <tok cat="NN">NFAT</tok> <tok cat="NN">complex</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PMA</tok></cons> <tok cat="CC">plus</tok> <cons cat="NP"><tok cat="NN">alphaCD28</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
863180908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">PMA/alphaCD28</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">produced</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">presence</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CsA</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
863180909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Collectively</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i55" role="SBJ"><tok cat="NN">NFAT</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i55" null="NONE"></cons></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i56" role="SBJ"><tok cat="NN">IL-2</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" ref="i56" null="NONE"></cons></cons></cons></cons></cons></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="NN">calcineurin</tok> <tok cat="JJ">independent</tok></cons> <tok cat="NN">manner</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
863182101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">Structural</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">functional</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">characterization</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">CD36</tok> <tok cat="NN">gene</tok> <tok cat="NN">promoter</tok></cons></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">identification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">proximal</tok> <cons cat="NP"><tok cat="NN">PEBP2/CBF</tok> <tok cat="NN">site</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@16-12-2010
863182102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">CD36</tok></cons> <cons cat="SBAR" ref="i30" null="ICH"></cons></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <cons cat="NP"><tok cat="NN">glycoprotein</tok></cons></cons> <cons cat="VP"><tok cat="VBN">composed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">single</tok> <tok cat="NN">polypeptide</tok> <tok cat="NN">chain</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" id="i30"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">interacts</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">thrombospondin</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><tok cat="NNS">collagens</tok> <tok cat="NN">type</tok> <cons cat="NP"><tok cat="CD">I</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">IV</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="VBN">oxidized</tok> <cons cat="NP"><tok cat="JJ">low</tok> <tok cat="NN">density</tok> <tok cat="NN">lipoprotein</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">fatty</tok> <tok cat="NNS">acids</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">anionic</tok> <tok cat="NNS">phospholipids</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">erythrocytes</tok></cons> <cons cat="VP"><tok cat="VBN">parasitized</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">Plasmodium</tok> <tok cat="NN">falciparum</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@42@@oe@16-12-2010
863182103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Its</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">restricted</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">few</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><tok cat="NNS">monocyte/macrophages</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
863182104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="NN">CD36</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">phagocytosis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">apoptotic</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">foam</tok> <tok cat="NN">cell</tok> <tok cat="NN">formation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">uptake</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">oxidized</tok> <tok cat="JJ">low</tok> <tok cat="NN">density</tok> <tok cat="NN">lipoprotein</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
863182105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">study</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">control</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CD36</tok></cons> <tok cat="NN">gene</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CD36</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
863182106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Transient</tok> <tok cat="NN">expression</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">5'-deletion</tok> <tok cat="NNS">fragments</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CD36</tok> <tok cat="NN">promoter</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">coupled</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">luciferase</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="QP"><tok cat="RB">as</tok> <tok cat="JJ">few</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><tok cat="CD">158</tok> <tok cat="NN">base</tok> <tok cat="NNS">pairs</tok> <tok cat="JJ">upstream</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">initiation</tok> <tok cat="NN">site</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">sufficient</tok></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">direct</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">monocyte-specific</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
863182107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Within</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">above</tok> <tok cat="NN">region</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">fragment</tok> <tok cat="NN">spanning</tok> <cons cat="NP"><tok cat="NNS">nucleotides</tok> <tok cat="CD">-158</tok> <tok cat="TO">to</tok> <tok cat="CD">-90</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">optimal</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
863182108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Biochemical</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">region</tok> <cons cat="NP"><tok cat="CD">-158/-90</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBN">revealed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="VBG">binding</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">polyomavirus</tok> <tok cat="JJ">enhancer-binding</tok> <tok cat="NN">protein</tok> <tok cat="JJ">2/core-binding</tok> <tok cat="NN">factor</tok> <tok cat="LRB">(</tok><tok cat="NN">PEBP2/CBF</tok><tok cat="RRB">)</tok> <tok cat="NN">family</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="NN">position</tok> <tok cat="CD">-103</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
863182109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Disruption</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">PEBP2/CBF</tok> <tok cat="NN">site</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">markedly</tok></cons> <cons cat="VP"><tok cat="VBD">diminished</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">PEBP2/CBF</tok> <tok cat="NNS">factors</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">constitutive</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CD36</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
863182110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">PEBP2/CBF</tok> <tok cat="NN">family</tok></cons></cons></cons></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="NNS">translocations</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">leukemia</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">myeloid-specific</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">myeloperoxidase</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">elastase</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">highlights</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">relevance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CD36</tok> <tok cat="NN">gene</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">PEBP2/CBF</tok> <tok cat="NN">family</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@59@@oe@16-12-2010
863196201@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Granulocyte-macrophage</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">JAK2</tok></cons> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p93fes</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">STAT1</tok> <tok cat="NN">p91</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT3</tok> <tok cat="NN">p92</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">polymorphonuclear</tok> <tok cat="NNS">leukocytes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@22@@oe@16-12-2010
863196202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Granulocyte-macrophage</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GM-CSF</tok></cons><tok cat="RRB">)</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">supports</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">proliferation</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="NN">activation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">hemopoietic</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">heterodimeric</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@26@@oe@16-12-2010
863196203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">GM-CSF</tok></cons> <tok cat="NN">receptor</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">devoid</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok></cons> <tok cat="JJ">enzymatic</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">GM-CSF-induced</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">polymorphonuclear</tok> <tok cat="NNS">leukocytes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PMN</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="JJ">functional</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">large</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
863196204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i33" role="SBJ"><tok cat="NN">JAK2</tok></cons> <cons cat="VP"><tok cat="VBZ">becomes</tok> <cons cat="VP"><tok cat="JJ">tyrosine-phosphorylated</tok> <cons cat="NP" ref="i33" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GM-CSF</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">PMN</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
863196205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NNS">transducers</tok> <tok cat="CC">and</tok> <tok cat="NNS">activators</tok> <tok cat="IN">of</tok> <tok cat="NN">transcription</tok> <tok cat="LRB">(</tok><tok cat="NN">STAT</tok><tok cat="RRB">)</tok> <tok cat="NN">family</tok> <tok cat="NNS">members</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT1</tok> <tok cat="NN">p91</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT3</tok> <tok cat="NN">p92</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">product</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">c-fps/fes</tok> <tok cat="NN">protooncogene</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBN">become</tok> <cons cat="ADJP" syn="COOD" role="PRD"><cons cat="ADJP"><tok cat="JJ">tyrosine-phosphorylated</tok> <cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GM-CSF</tok></cons> <tok cat="NN">stimulation</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">physically</tok></cons> <cons cat="ADJP"><tok cat="VBN">associated</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GM-CSF</tok></cons> <tok cat="NN">receptor</tok> <tok cat="NN">beta</tok> <tok cat="JJ">common</tok> <tok cat="NN">subunit</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">JAK2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@50@@oe@16-12-2010
863196206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons> <cons cat="NP" id="i8" role="SBJ"><tok cat="NN">GM-CSF</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">JAK2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p93fes</tok></cons></cons> <tok cat="JJ">catalytic</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
863196207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">association</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GM-CSF</tok> <tok cat="NN">receptor</tok> <tok cat="NN">beta</tok> <tok cat="JJ">common</tok> <tok cat="NN">subunit</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">JAK2</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">ligand-dependent</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
863196208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Finally</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">GM-CSF</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NN">complex</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">p91</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p92</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
863196209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">identify</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">new</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok> <tok cat="NN">pathway</tok></cons> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">GM-CSF</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">rapid</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">GM-CSF-stimulated</tok> <tok cat="NN">PMN</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
863267001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Id2</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Id3</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><cons cat="NP" id="i1"><tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">lymphocytes</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@10@@oe@16-12-2010
863267002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="JJ">Helix-loop-helix</tok> <tok cat="LRB">(</tok><tok cat="JJ">HLH</tok><tok cat="RRB">)</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">cellular</tok> <cons cat="NP"><tok cat="NN">growth</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@16-12-2010
863267003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Id</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="NN">DNA</tok> <cons cat="NP"><tok cat="NN">binding</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP"><tok cat="JJ">HLH</tok> <tok cat="NNS">proteins</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">dominantly</tok> <tok cat="JJ">negative</tok></cons> <tok cat="NN">fashion</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBP">regulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NNS">activities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">basic</tok> <tok cat="JJ">HLH</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
863267004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">northern</tok> <tok cat="NN">hybridization</tok></cons></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Id2</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Id3</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons><cons cat="NP" id="i3"><tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">leukemia/lymphoma</tok> <tok cat="NNS">lines</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">patient</tok> <tok cat="NNS">samples</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBG">resting</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="JJ">normal</tok> <tok cat="JJ">human</tok> <tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PBL</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
863267005@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">Id2</tok> <tok cat="NN">mRNA</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">abundantly</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="CD">5/12</tok> <tok cat="NN">T-cell</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">3/4</tok> <tok cat="NN">B-cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">Id3</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="CD">4/12</tok> <tok cat="NN">T-cell</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">3/4</tok> <tok cat="NN">B-cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
863267006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Interestingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i6" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Id2</tok></cons><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">Id3</tok></cons><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i20"><tok cat="NN">mRNA</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="CD">4/5</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="VP"><tok cat="VBN">infected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <cons cat="NP" id="i42"><tok cat="NN">type</tok> <tok cat="CD">I</tok> <tok cat="LRB">(</tok><tok cat="NN">HTLV-I</tok><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="PRN" id="i43"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ATL-1k</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">MT-2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">S-LB1</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons map="i42" cat="NP"><tok cat="NN">type</tok> <tok cat="CD">II</tok></cons> <cons map="i43" cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Mo</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
863267007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">Another</tok> <tok cat="JJ">unexpected</tok> <tok cat="NN">finding</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="SBAR" role="PRD"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NNS">leukemias</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NNS">lines</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">often</tok></cons> <cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Id2</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">Id3</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
863267008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="VBG">resting</tok> <tok cat="NN">PBL</tok></cons> <cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">prominent</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Id2</tok> <tok cat="NN">mRNA</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">Id3</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
863267009@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="PP"><tok cat="IN">Upon</tok> <cons cat="NP"><tok cat="NN">PHA-stimulation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Id2</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">decreased</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Id3</tok></cons> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">biphasic</tok> <tok cat="NNS">kinetics</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
863267010@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">Taken</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">together</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok> <tok cat="JJ">unexpected</tok> <tok cat="NNS">findings</tok> <cons cat="S" ref="i9" null="ICH"></cons></cons><cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">require</tok> <cons cat="NP"><tok cat="JJ">further</tok> <tok cat="NN">analysis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="S" syn="COOD" id="i9"><cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">1</tok><tok cat="RRB">)</tok></cons> <cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Id2</tok> <tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">often</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">lymphocytic</tok> <tok cat="NN">transformation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">HTLV-I</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><tok cat="CD">-II</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S" syn="COOD"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">2</tok><tok cat="RRB">)</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">T-cells</tok></cons> <cons cat="ADVP"><tok cat="RB">usually</tok></cons> <cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Id2</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">Id3</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">B-cells</tok></cons> <cons cat="ADVP"><tok cat="RB">often</tok></cons> <cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP"><tok cat="DT">both</tok></cons> <cons cat="ADVP"><tok cat="RB">simultaneously</tok></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S" syn="COOD"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">3</tok><tok cat="RRB">)</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">non-dividing</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">normal</tok></cons></cons> <cons cat="NP"><tok cat="NN">PBL</tok></cons></cons> <cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Id2</tok></cons> <cons cat="NP" null="QSTN"></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">no</tok> <cons cat="NP"><tok cat="NN">Id3</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">onset</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NN">proliferation</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Id2</tok> <tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">decrease</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Id3</tok> <tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">increase</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="ADJP"><tok cat="RB">closely</tok> <tok cat="JJ">related</tok></cons> <cons cat="NP"><tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">disparate</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@101@@oe@16-12-2010
863299901@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">CNI-1493</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><tok cat="JJ">monocyte/macrophage</tok> <tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">suppression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">translation</tok> <tok cat="NN">efficiency</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@12@@oe@16-12-2010
863299902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNF</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">mediates</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">wide</tok> <tok cat="NN">variety</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">disease</tok> <tok cat="NNS">states</tok></cons></cons></cons> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">septic</tok> <tok cat="NN">shock</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">acute</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">chronic</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">inflammation</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">cachexia</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@25@@oe@16-12-2010
863299903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Recently</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">multivalent</tok> <tok cat="NN">guanylhydrazone</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CNI-1493</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBN">developed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">macrophage</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">suppress</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">TNF</tok></cons> <tok cat="NN">production</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="NN">protect</tok> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">tissue</tok> <tok cat="NN">inflammation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">endotoxin</tok> <tok cat="NN">lethality</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNP">Bianchi</tok><tok cat="COMMA">,</tok> <tok cat="NNP">M.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Ulrich</tok><tok cat="COMMA">,</tok> <tok cat="NNP">P.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Bloom</tok><tok cat="COMMA">,</tok> <tok cat="NNP">O.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Meistrell</tok><tok cat="COMMA">,</tok> <tok cat="NNP">M.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Zimmerman</tok><tok cat="COMMA">,</tok> <tok cat="NNP">G.A.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Schmidtmayerova</tok><tok cat="COMMA">,</tok> <tok cat="NNP">H.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Bukrinsky</tok><tok cat="COMMA">,</tok> <tok cat="NNP">M.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Donnelley</tok><tok cat="COMMA">,</tok> <tok cat="NNP">T.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Bucala</tok><tok cat="COMMA">,</tok> <tok cat="NNP">R.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Sherry</tok><tok cat="COMMA">,</tok> <tok cat="NNP">B.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Manogue</tok><tok cat="COMMA">,</tok> <tok cat="NNP">K.R.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Tortolani</tok><tok cat="COMMA">,</tok> <tok cat="NNP">A.J.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Cerami</tok><tok cat="COMMA">,</tok> <tok cat="NNP">A.</tok></cons><tok cat="CC">&amp;</tok> <cons cat="NP"><tok cat="NNP">Tracey</tok><tok cat="COMMA">,</tok> <tok cat="NNP">K.J.</tok></cons></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">1995</tok></cons><tok cat="RRB">)</tok></cons> <cons cat="NP"><tok cat="NNP">Mol.Med.1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">254-266</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNP">Bianchi</tok><tok cat="COMMA">,</tok> <tok cat="NNP">M.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Bloom</tok><tok cat="COMMA">,</tok> <tok cat="NNP">O.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Raabe</tok><tok cat="COMMA">,</tok> <tok cat="NNP">T.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Cohen</tok><tok cat="COMMA">,</tok> <tok cat="NNP">P.</tok> <tok cat="NNP">S.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Chesney</tok><tok cat="COMMA">,</tok> <tok cat="NNP">J.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Sherry</tok><tok cat="COMMA">,</tok> <tok cat="NNP">B.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Schmidtmayerova</tok><tok cat="COMMA">,</tok> <tok cat="NNP">H.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Zhang</tok><tok cat="COMMA">,</tok> <tok cat="NNP">X.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Bukrinsky</tok><tok cat="COMMA">,</tok> <tok cat="NNP">M.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Ulrich</tok><tok cat="COMMA">,</tok> <tok cat="NNP">P.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Cerami</tok><tok cat="COMMA">,</tok> <tok cat="NNP">A.</tok></cons><tok cat="CC">&amp;</tok> <cons cat="NP"><tok cat="NNP">Tracey</tok><tok cat="COMMA">,</tok> <tok cat="NNP">J.</tok></cons></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">1996</tok></cons><tok cat="RRB">)</tok></cons> <cons cat="NP"><tok cat="NNP">J.Exp.Med.</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">press</tok></cons></cons></cons></cons><tok cat="RRB">]</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@150@@oe@16-12-2010
863299904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="VP"><tok cat="VBN">elucidated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">CNI-1493</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><tok cat="NN">macrophage</tok> <cons cat="NP"><tok cat="NN">TNF</tok></cons> <tok cat="NN">synthesis</tok></cons> <cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">acts</tok> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">suppression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">TNF</tok></cons> <tok cat="NN">translation</tok> <tok cat="NN">efficiency</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
863299905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">CNI-1493</tok></cons> <cons cat="VP"><tok cat="VBD">blocked</tok> <cons cat="NP" syn="COOD"><tok cat="CC">neither</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lipopolysaccharide</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LPS</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="JJ">-induced</tok> <tok cat="NNS">increases</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TNF</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons> <tok cat="CC">nor</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">macrophages</tok></cons> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">15</tok> <tok cat="NN">min</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">LPS</tok> <tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">CNI-1493</tok></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">interfere</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">NF-kappa</tok> <tok cat="JJ">B-mediated</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TNF</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@52@@oe@16-12-2010
863299906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">synthesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">26-kDa</tok> <tok cat="NN">membrane</tok> <tok cat="NN">form</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TNF</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">effectively</tok></cons> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">CNI-1493</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
863299907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="RB">Further</tok> <tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">translational</tok> <tok cat="NN">suppression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TNF</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">given</tok> <cons cat="NP" null="NONE"></cons><cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">experiments</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">chloram-phenicol</tok> <tok cat="NN">acetyltransferase</tok> <tok cat="LRB">(</tok><tok cat="NN">CAT</tok><tok cat="RRB">)</tok> <tok cat="VBZ">constructs</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">elements</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TNF</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i11"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i11" id="i12" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">TNF</tok></cons> <tok cat="JJ">translational</tok> <tok cat="NN">regulation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
863299908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i13" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="CC">Both</tok> <tok cat="DT">the</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">5'</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">3'</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="JJ">untranslated</tok> <tok cat="NNS">regions</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TNF</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i13" null="NONE"></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">elicit</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">maximal</tok> <tok cat="JJ">translational</tok> <tok cat="NN">suppression</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">CNI-1493</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
863299909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Identification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">target</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i31"><tok cat="IN">through</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">CNI- 1493</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">TNF</tok></cons> <tok cat="NN">translation</tok></cons> <cons cat="PP" ref="i31" null="T"></cons></cons></cons></cons></cons></cons></cons><cons cat="VP"><tok cat="MD">should</tok> <cons cat="VP"><tok cat="VB">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">insight</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">macrophage</tok> <tok cat="NN">activation</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">mechanisms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">inflammation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
863441301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="JJ">STAT-related</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="NN">leukemia</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
863441302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i16" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok> <tok cat="NN">pathway</tok></cons> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">many</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">elaborated</tok><cons cat="NP" ref="i16" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
863441303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">JAK</tok> <tok cat="NNS">kinases</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NNS">transducers</tok> <tok cat="CC">and</tok> <tok cat="NNS">activators</tok> <tok cat="IN">of</tok> <tok cat="NN">transcription</tok> <tok cat="LRB">(</tok><tok cat="NN">STAT</tok><tok cat="RRB">)</tok> <tok cat="NNS">factors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><tok cat="JJ">essential</tok> <tok cat="NNS">components</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
863441304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">report</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">describe</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">constitutively</tok> <tok cat="VBN">activated</tok></cons> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NNS">factors</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="NN">leukemia</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
863441305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">used</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">oligonucleotide</tok> <tok cat="NNS">probes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">beta-casein</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IRF-1</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">gene</tok> <tok cat="NNS">promoters</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ISRE</tok> <tok cat="NN">probe</tok></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">detect</tok> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="NN">leukemia</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">bandshift</tok> <tok cat="NNS">assays</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
863441306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="JJ">Specific</tok> <tok cat="NN">DNA</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">complex</tok></cons> <tok cat="NN">formation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">probes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">beta-casein</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IRF-1</tok> <tok cat="NN">gene</tok> <tok cat="NNS">promoters</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ISRE</tok> <tok cat="NN">oligonucleotide</tok> <tok cat="NN">probe</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i7"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">lymphoblastic</tok> <tok cat="NN">leukemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ALL</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">leukemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AML</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">investigated</tok><cons cat="NP" ref="i9" null="NONE"></cons><cons cat="ADVP" ref="i7" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@16-12-2010
863441307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">used</tok> <cons cat="NP"><tok cat="JJ">nonradioactive</tok> <tok cat="NNS">oligonucleotides</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="NNS">competitors</tok></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">show</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">specificity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">complex</tok> <tok cat="NN">formation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
863441308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="JJ">Specific</tok> <cons cat="NP"><tok cat="NNS">antibodies</tok></cons></cons> <cons cat="VP"><tok cat="VBN">directed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">individual</tok> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">supershift</tok> <tok cat="NNS">experiments</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
863441309@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" syn="COOD" id="i12" role="SBJ"><cons cat="NP"><tok cat="NN">STAT5-</tok> <cons cat="ADJP" null="QSTN"></cons><cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">STAT1-related</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">factors</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">ALL</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">STAT1-</tok><cons cat="ADJP" null="QSTN"></cons><cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">STAT3-</tok><cons cat="ADJP" null="QSTN"></cons><cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">STAT5-related</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NNS">proteins</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">cell</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">AML</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
863441310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Since</tok> <cons cat="S"><cons cat="NP" id="i13" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons></cons> <cons cat="SBAR" func="ADV" adv="TMP"><tok cat="IN">before</tok> <cons cat="S"><cons cat="NP" id="i14" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">extracted</tok><cons cat="NP" ref="i14" null="NONE"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i15" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">factors</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons><cons cat="NP" ref="i15" null="NONE"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
863441311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">likely</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">constitutive</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NNS">proteins</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">part</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">events</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">leukemogenesis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
863441801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">RAR-RXR</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">RXR-RXR</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">pathway</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">signaling</tok> <tok cat="NN">growth</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">CD34+</tok> <tok cat="JJ">erythroid</tok> <tok cat="NN">progenitor</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@16-12-2010
863441802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Previous</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RA</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">similar</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor-alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">act</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">bifunctional</tok> <tok cat="NN">regulator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">growth</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">bone</tok> <tok cat="NN">marrow</tok> <tok cat="NNS">progenitors</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">stimulate</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">granulocyte-macrophage</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GM-CSF</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COLON">-</tok> <cons cat="ADJP" null="QSTN"></cons><cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-3</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-3</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="JJ">-induced</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">GM</tok> <tok cat="NN">colony</tok> <tok cat="NN">formation</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="ADVP"><tok cat="RB">potently</tok></cons> <cons cat="VP"><tok cat="VBP">inhibit</tok> <cons cat="NP"><tok cat="JJ">G-CSF-induced</tok> <tok cat="NN">growth</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@62@@oe@16-12-2010
863441803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok><tok cat="COMMA">,</tok></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">highly</tok> <tok cat="VBN">enriched</tok></cons> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">CD34+</tok></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><tok cat="JJ">Lin-</tok> <tok cat="JJ">murine</tok> <tok cat="NN">bone</tok> <tok cat="NN">marrow</tok> <tok cat="NN">progenitor</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">demonstrates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="JJ">potent</tok> <tok cat="JJ">inhibitory</tok></cons> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">9-cis-RA</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">burst-forming</tok> <tok cat="JJ">unit-erythroid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">BFU-E</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">colony</tok> <tok cat="NN">formation</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">regardless</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">cytokine</tok></cons> <tok cat="NN">stimulating</tok> <tok cat="NN">growth</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
863441804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Specifically</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">9-cis-RA</tok></cons> <cons cat="ADVP"><tok cat="RB">potently</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">growth</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">BFU-E</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP" id="i40"><tok cat="NN">erythropoietin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Epo</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">100</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><cons cat="PP"><cons cat="NP"><cons cat="PP"><cons cat="NP"><cons map="i40" cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">stem</tok> <tok cat="NN">cell</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SCF</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">+</tok> <cons cat="NP"><tok cat="NN">Epo</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">92</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><cons cat="PP"><cons cat="NP"><cons cat="PP"><cons map="i40" cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-3</tok></cons> <tok cat="CC">+</tok> <cons cat="NP"><tok cat="NN">Epo</tok></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">97</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><cons cat="PP"><cons cat="NP"><cons cat="PP"><cons map="i40" cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-4</tok></cons> <tok cat="CC">+</tok> <cons cat="NP"><tok cat="NN">Epo</tok></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">88</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="PP"><cons cat="NP"><cons cat="PP"><cons map="i40" cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-9</tok></cons> <tok cat="CC">+</tok> <cons cat="NP"><tok cat="NN">Epo</tok></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">100</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@58@@oe@16-12-2010
863441805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="JJ">Erythroid</tok> <tok cat="NN">colony</tok> <tok cat="NN">growth</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i6" null="NONE"></cons></cons><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i7"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="JJ">CD34+</tok> <tok cat="NNS">progenitors</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">seeded</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="CD">one</tok> <tok cat="NN">cell</tok></cons></cons> <cons cat="PP"><tok cat="IN">per</tok> <cons cat="NP"><tok cat="NN">well</tok></cons></cons><cons cat="ADVP" ref="i7" null="T"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">direct</tok> <tok cat="NN">action</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">RA</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
863441806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">synthetic</tok> <tok cat="NNS">ligands</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">RARs</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">retinoid</tok> <tok cat="NN">X</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">RXRs</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">selectively</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">bind</tok> <cons cat="NP" ref="i24" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">activate</tok> <cons cat="NP" ref="i24" null="RNR"></cons></cons><cons cat="NP" syn="COOD" id="i24"><cons cat="NP"><cons cat="NP"><tok cat="NN">RAR-RXR</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">RXR-RXR</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NNS">dimers</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">dissected</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="NN">retinoid</tok> <tok cat="NN">response</tok> <tok cat="NNS">pathways</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">normal</tok></cons> <cons cat="NP"><tok cat="JJ">myeloid</tok> <cons cat="NP" ref="i25" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NN">progenitor</tok> <cons cat="NP" ref="i25" null="RNR"></cons></cons><cons cat="NP" id="i25"><tok cat="NN">cell</tok> <tok cat="NN">growth</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@16-12-2010
863441807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Transactivation</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">RAR</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Ro</tok> <tok cat="CD">13-7410</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" ref="i26" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">RXR</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Ro</tok> <tok cat="CD">25-6603</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Ro</tok> <tok cat="CD">25-7386</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" ref="i26" null="RNR"></cons></cons><cons cat="NP" id="i26"><tok cat="NNS">ligands</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">highly</tok> <tok cat="JJ">selective</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="CD">100</tok> <tok cat="NN">nmol/L</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
863441808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">At</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">concentration</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">Ro</tok> <tok cat="CD">13-7410</tok></cons> <cons cat="ADVP"><tok cat="RB">potently</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">G-CSF-stimulated</tok> <tok cat="JJ">myeloid</tok> <cons cat="NP" ref="i27" null="RNR"></cons></cons><cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">SCF</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">+</tok> <cons cat="ADJP"><tok cat="JJ">Epo-induced</tok></cons></cons> <tok cat="JJ">erythroid</tok> <cons cat="NP" ref="i27" null="RNR"></cons></cons><cons cat="NP" id="i27"><tok cat="NN">colony</tok> <tok cat="NN">growth</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
863441809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">At</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NN">concentration</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Ro</tok> <tok cat="CD">25-6603</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Ro</tok> <tok cat="CD">25-7386</tok></cons></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">little</tok></cons> <cons cat="NP" ref="i28" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">no</tok> <cons cat="NP" ref="i28" null="RNR"></cons></cons><cons cat="NP" id="i28"><tok cat="NN">effect</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">G-CSF-induced</tok> <tok cat="NN">colony</tok> <tok cat="NN">formation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="CD">75</tok><tok cat="NN">%</tok></cons> <cons cat="PP" ref="i30" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">53</tok><tok cat="NN">%</tok></cons><cons cat="PP" ref="i30" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP" id="i30"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">SCF</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">+</tok> <cons cat="ADJP"><tok cat="JJ">Epo-stimulated</tok></cons></cons> <tok cat="NN">BFU-E</tok> <tok cat="NN">colony</tok> <tok cat="NN">growth</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
863441810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">RAR-RXR</tok></cons> <tok cat="NN">response</tok> <tok cat="NN">pathway</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">signal</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">growth</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">normal</tok></cons> <cons cat="NP"><tok cat="NN">bone</tok> <tok cat="NN">marrow</tok> <tok cat="JJ">myeloid</tok> <cons cat="NP" ref="i29" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NN">progenitor</tok> <cons cat="NP" ref="i29" null="RNR"></cons></cons><cons cat="NP" id="i29"><tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
863441811@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">unique</tok> <tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">RXR-RXR</tok></cons> <tok cat="NN">pathway</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">mediating</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">growth</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">erythroid</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NN">progenitor</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
863443801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">A-myb</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-myb</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">B-myb</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">restricted</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Burkitt</tok><tok cat="POS">'s</tok> <tok cat="NN">lymphoma</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">sIg+</tok> <tok cat="JJ">B-acute</tok> <tok cat="JJ">lymphoblastic</tok> <tok cat="NN">leukemia</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">subset</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">chronic</tok> <tok cat="JJ">lymphocytic</tok></cons> <tok cat="NNS">leukemias</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@30@@oe@16-12-2010
863443802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">A-myb</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">encodes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">related</tok> <cons cat="ADVP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADVP"><tok cat="RB">functionally</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">structurally</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">v-myb</tok> <tok cat="NN">oncogene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
863443803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="VBG">Following</tok> <cons cat="NP"><tok cat="PRPP">our</tok> <tok cat="NNS">observations</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">A-myb</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">restricted</tok> <tok cat="NN">subset</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <tok cat="NN">phenotype</tok> <cons cat="NP"><tok cat="NN">CD38+</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CD39-</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">slgM-</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pattern</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">A-myb</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">neoplastic</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBG">representating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">whole</tok> <tok cat="NN">spectrum</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">compared</tok> <cons cat="NP"><tok cat="PRP">it</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-myb</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">B-myb</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@57@@oe@16-12-2010
863443804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">panel</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">32</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><tok cat="NN">A-myb</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="UCP" syn="COOD"><cons cat="ADVP"><tok cat="RB">very</tok> <tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJS">most</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Burkitt</tok><tok cat="POS">'s</tok> <tok cat="NN">lymphoma</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">BL</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="JJ">weak</tok> <cons cat="PP" ref="i21" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">negative</tok> <cons cat="PP" ref="i21" null="RNR"></cons></cons><cons cat="PP" id="i21"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">2</tok> <tok cat="NN">pre-B</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">lymphoblastic</tok> <tok cat="NN">leukemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ALL</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">4</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">non-Hodgkin</tok><tok cat="POS">'s</tok> <tok cat="NN">lymphoma</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NHL</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">6</tok> <cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="JJ">virus-immortalized</tok> <tok cat="JJ">lymphoblastoid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">6</tok> <cons cat="NP"><tok cat="NN">myeloma</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@58@@oe@16-12-2010
863443805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Protein</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">paralleled</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">RNA</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@16-12-2010
863443806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">A-myb</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">49</tok> <tok cat="JJ">fresh</tok> <tok cat="NNS">cases</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">leukemias</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
863443807@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="PP"><tok cat="IN">Among</tok> <cons cat="NP"><tok cat="CD">24</tok> <tok cat="NN">ALL</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i40" role="SBJ"><tok cat="CD">6</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="PP" id="i41" role="PRD"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">null</tok> <cons cat="NP" null="QSTN"></cons></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="S"><cons map="i40" cat="NP" role="SBJ"><tok cat="CD">11</tok></cons> <cons map="i41" cat="PP" role="PRD"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">common</tok> <tok cat="NN">type</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">all</tok> <tok cat="DT">these</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">negative</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">A-myb</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">other</tok> <tok cat="NN">hand</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="CD">7</tok> <tok cat="NN">B-ALL</tok> <tok cat="NNS">cases</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="ADJP"><tok cat="JJ">slg+</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok> <tok cat="JJ">fresh</tok> <tok cat="NN">BL</tok> <tok cat="NN">case</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">bone</tok> <tok cat="NN">marrow</tok> <tok cat="NN">infiltration</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP"><tok cat="NN">A-myb</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@52@@oe@16-12-2010
863443808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="NN">A-myb</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">undetectable</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="CD">4</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">prolymphocytic</tok> <tok cat="NNS">leukemias</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PLL</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">5/20</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">25</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">chronic</tok> <tok cat="JJ">lymphocytic</tok> <tok cat="NN">leukemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CLL</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">samples</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
863443809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">latter</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="NN">A-myb</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">correlate</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">phenotype</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">clinical</tok> <tok cat="NN">stage</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
863443810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">studied</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">progression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok> <tok cat="NN">case</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CLL</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="NN">Richter</tok><tok cat="POS">'s</tok> <tok cat="NN">syndrome</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Richter</tok><tok cat="POS">'s</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><cons cat="NP"><tok cat="RB">about</tok> <tok cat="RB">25-fold</tok></cons> <tok cat="JJR">less</tok></cons> <cons cat="NP"><tok cat="NN">A-myb</tok> <tok cat="NN">RNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CLL</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NN">patient</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
863443811@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">pattern</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-myb</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">B-myb</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">clearly</tok> <tok cat="JJ">distinct</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">A-myb</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
863443812@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">C-myb</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">B-myb</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">all</tok> <tok cat="JJ">neoplastic</tok> <tok cat="NNS">groups</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">except</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">CLL</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
863443813@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">A-myb</tok></cons> <tok cat="NN">expression</tok><tok cat="COMMA">,</tok></cons> <cons cat="PP"><tok cat="IN">unlike</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-myb</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">B-myb</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">restricted</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">subset</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">neoplasias</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">particular</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">BL</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">slg+B-ALL</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">representative</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">specific</tok> <tok cat="NN">stage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
863443814@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="NN">may</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">part</tok></cons></cons> <cons cat="VP"><tok cat="VBP">reflect</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">A-myb</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">normal</tok> <tok cat="JJ">germinal</tok> <tok cat="NN">center</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i11"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">normal</tok> <tok cat="NN">counterpart</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="VBN">transformed</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
863443815@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBN">presented</tok> <cons cat="NP" null="NONE"></cons></cons></cons><cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBP">support</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">this</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">differentiation</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">perhaps</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">transformation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">some</tok> <tok cat="NNS">neoplasias</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
863444001@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p13MTCP1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">restricted</tok> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="VB">mature</tok> <tok cat="NN">T-cell</tok> <tok cat="NNS">proliferations</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">t(X;14)</tok> <tok cat="NNS">translocations</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
863444002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" id="i5" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="JJ">prolymphocytic</tok> <tok cat="NN">leukemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">T-PLL</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">rare</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="NN">T-cell</tok> <tok cat="NNS">leukemias</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">ataxia</tok> <tok cat="NN">telangiectasia</tok> <tok cat="JJ">clonal</tok> <tok cat="NN">proliferation</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">related</tok> <tok cat="NN">condition</tok></cons> <cons cat="VP"><tok cat="NN">occurring</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="VP"><tok cat="VBG">suffering</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">ataxia</tok> <tok cat="NN">telangiectasia</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">translocations</tok></cons> <cons cat="VP"><tok cat="VBG">involving</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">14q32.1</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">Xq28</tok> <tok cat="NNS">regions</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHADVP" id="i4"><tok cat="WRB">where</tok></cons> <cons cat="SINV"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">located</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TCL1</tok> <tok cat="CC">and</tok> <tok cat="NN">MTCP1</tok> <tok cat="JJ">putative</tok> <tok cat="NNS">oncogenes</tok></cons></cons><cons cat="ADVP" ref="i4" null="T"></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@56@@oe@16-12-2010
863444003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">MTCP1</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">t(X;14)(q28;q11)</tok> <tok cat="NN">translocation</tok></cons></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NN">T-cell</tok> <tok cat="NNS">proliferations</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
863444004@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Alternative</tok> <tok cat="NN">splicing</tok></cons> <cons cat="VP"><tok cat="VBZ">generates</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="NN">type</tok> <cons cat="NP"><tok cat="NN">A</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">B</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">transcripts</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">potentially</tok></cons> <cons cat="VP"><tok cat="VBP">encode</tok> <cons cat="NP"><tok cat="CD">two</tok> <cons cat="ADJP"><tok cat="RB">entirely</tok> <tok cat="JJ">distinct</tok></cons> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">type</tok> <cons cat="NP"><tok cat="NN">A</tok> <tok cat="NNS">transcripts</tok></cons></cons> <cons cat="VP"><tok cat="VBP">code</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">small</tok> <cons cat="NP"><tok cat="JJ">mitochondrial</tok> <tok cat="NN">protein</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p8MTCP1</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">type</tok> <tok cat="NN">B</tok> <tok cat="NNS">transcripts</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">additional</tok> <cons cat="NP"><tok cat="NN">open</tok> <tok cat="NN">reading</tok> <tok cat="NN">frame</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VBP">code</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">107</tok> <tok cat="JJ">amino-acid</tok> <tok cat="NN">protein</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p13MTCP1</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@49@@oe@16-12-2010
863444005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="ADJP"><tok cat="RB">recently</tok> <tok cat="VBN">cloned</tok></cons> <cons cat="NP"><tok cat="NN">TCL1</tok> <tok cat="NN">gene</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">translocations</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">inversions</tok></cons></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NNS">proliferations</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">codes</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">14-kD</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">displays</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">significant</tok> <tok cat="NN">homology</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">p13MTCP1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
863444006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">generated</tok> <cons cat="NP"><tok cat="NN">rabbit</tok> <tok cat="NNS">antisera</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">putative</tok> <cons cat="NP"><tok cat="NN">p13MTCP1</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">screened</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">p13MTCP1</tok></cons> <tok cat="JJ">normal</tok> <tok cat="JJ">lymphoid</tok> <tok cat="NNS">tissues</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">33</tok> <tok cat="NNS">cases</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">immature</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">mature</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="JJ">lymphoid</tok> <tok cat="NN">T-cell</tok> <tok cat="NNS">proliferations</tok></cons></cons></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">sensitive</tok> <tok cat="NN">Western</tok> <tok cat="NN">blot</tok> <tok cat="NN">assay</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
863444007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">MTCP1</tok> <tok cat="NN">locus</tok></cons> <tok cat="NN">configuration</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">Southern</tok> <tok cat="NN">blot</tok> <tok cat="NN">analysis</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
863444008@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i13" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">p13MTCP1</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="PP" id="i30"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">three</tok> <tok cat="NN">T-cell</tok> <tok cat="NNS">proliferations</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">MTCP1</tok> <tok cat="NNS">rearrangements</tok></cons> <cons cat="PP"><tok cat="IN">because</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">t(X;14)</tok> <tok cat="NNS">translocations</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons map="i30" cat="PP" syn="COOD"><tok cat="CC">neither</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">normal</tok> <cons cat="NP"><tok cat="VBG">resting</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons> <tok cat="CC">nor</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">other</tok> <cons cat="NP"><tok cat="NN">T-cell</tok></cons> <tok cat="NNS">leukemias</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
863444009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">support</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">hypothesis</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">p13MTCP1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p14TCL1</tok></cons></cons> <cons cat="VP"><tok cat="VBP">form</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">new</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">family</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i14"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i14" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">key</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathogenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">T-PLL</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">related</tok> <tok cat="NNS">conditions</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
863552301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Reversible</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">monoblastic</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">ML-9</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">myosin</tok> <tok cat="JJ">light</tok> <tok cat="NN">chain</tok> <tok cat="NN">kinase</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
863552302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="JJ">Human</tok> <tok cat="JJ">monoblastic</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i5" null="NONE"></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">differentiate</tok> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">various</tok> <tok cat="NNS">agents</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@16-12-2010
863552303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">1-(5-chloronaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine</tok> <tok cat="NN">hydrochloride</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ML-9</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">myosin</tok> <tok cat="JJ">light</tok> <tok cat="NN">chain</tok> <tok cat="NN">kinase</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">monocytoid</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="NP"><tok cat="NN">U937</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">THP-1</tok></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">myeloblastic</tok> <tok cat="JJ">leukemic</tok> <tok cat="NN">ML-1</tok> <tok cat="NN">cell</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">erythroleukemia</tok> <tok cat="NN">K562</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
863552304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="ADVP"><tok cat="RB">further</tok></cons> <cons cat="VP"><tok cat="VBD">analyzed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ML-9</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">comparison</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">1</tok> <tok cat="NN">alpha, 25-dihydroxyvitamin</tok> <tok cat="NN">D3</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">VD3</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">typical</tok> <tok cat="NN">inducer</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
863552305@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">ML-9</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nitroblue</tok> <tok cat="NN">tetrazolium</tok> <tok cat="LRB">(</tok><tok cat="NN">NBT</tok><tok cat="RRB">)</tok><tok cat="JJ">-reducing</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">U937</tok> <tok cat="NN">cell</tok></cons></cons></cons> <cons cat="ADVP"><cons cat="ADVP"><tok cat="RBR">more</tok> <tok cat="RB">rapidly</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">than</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">VD3</tok></cons><cons cat="VP" null="QSTN"></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">differentiation</tok> <tok cat="NN">marker</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">incubation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ML-9</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">VD3</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">4</tok> <tok cat="NNS">hours</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">1</tok> <tok cat="NN">day</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
863552306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">ML-9</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">alpha-naphthyl</tok> <tok cat="NN">acetate</tok> <tok cat="NN">esterase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ANAE</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">activity</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">another</tok> <tok cat="JJ">monocytic</tok> <tok cat="NN">differentiation</tok> <tok cat="NN">marker</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><cons cat="ADJP"><tok cat="JJR">more</tok> <tok cat="RB">rapidly</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">than</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">VD3</tok></cons><cons cat="VP" null="QSTN"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
863552307@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">maximum</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">markers</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">ML-9</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">comparable</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">VD3</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">removal</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ML-9</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">medium</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">washing</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">expressions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">theses</tok> <tok cat="NNS">markers</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">decreased</tok> <cons cat="PP" adv="TMP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="CD">4</tok> <tok cat="NNS">hours</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">reached</tok> <cons cat="NP"><tok cat="JJ">basal</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="CD">1</tok> <tok cat="NN">day</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">ML-9</tok><tok cat="POS">'s</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">differentiation-associated</tok> <tok cat="NNS">phenotypes</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">reversible</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@60@@oe@16-12-2010
863552308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">growth</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">ML-9</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">reversible</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
863552309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="JJ">Similar</tok> <tok cat="NNS">effects</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">another</tok> <tok cat="NN">line</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">monoblastic</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">THP-1</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
863552310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">ML-9</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">little</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">no</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">effect</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">morphology</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">monocyte-macrophage</tok> <tok cat="JJ">lineage-associated</tok> <tok cat="NN">surface</tok> <tok cat="NN">antigen</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CD14</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">some</tok> <tok cat="NN">extent</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
863552311@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="JJ">Irreversible</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">VD3</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">down</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-myc</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">upregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-jun</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">ML-9</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">oncogenes</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">appreciably</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
863552312@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">ML-9-induced</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">reversible</tok></cons> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i15"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i16" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">cultured</tok> <cons cat="NP" ref="i16" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <tok cat="VBN">cultured</tok> <tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ML-9</tok></cons> <tok cat="CC">plus</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">anti-cancer</tok> <tok cat="NN">drug</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">1-beta-D-arabino-furanosylcytosine</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">daunomycin</tok></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i15" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
863552313@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">became</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">irreversible</tok><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">simultaneous</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">dexamethasone</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">transforming</tok> <tok cat="NN">growth</tok> <tok cat="NN">factor-beta</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TGF-beta</tok> <tok cat="CD">1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">growth</tok> <tok cat="NN">arrest</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">G0/G1</tok> <tok cat="NN">phase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
863552314@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">ML-9</tok></cons> <cons cat="VP"><tok cat="MD">should</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">useful</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">studying</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
863946101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Lymphoid</tok> <tok cat="NN">cell</tok> <tok cat="NN">resistance</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">infection</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@9@@oe@16-12-2010
863946102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">humans</tok></cons> <cons cat="VP"><tok cat="VBN">infected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">virus</tok></cons></cons></cons></cons></cons> <cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">disproportionate</tok> <tok cat="NN">severity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">signs</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">symptoms</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">illness</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="VBN">compared</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">fraction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">CD4+</tok> <tok cat="JJ">infected</tok> <tok cat="NN">T-lymphoid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@29@@oe@16-12-2010
863946103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">part</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">this</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">due</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">altered</tok> <cons cat="NP"><tok cat="JJ">intercellular</tok> <tok cat="NN">signalling</tok> <tok cat="NNS">systems</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
863946104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">Glucocorticoids</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">well</tok> <tok cat="JJ">known</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">vitality</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">function</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">lymphoid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
863946105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NNS">infections</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">often</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">elevated</tok> <tok cat="JJ">circulating</tok></cons> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cortisol</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">some</tok> <tok cat="NN">misfunction</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">systems</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">maintain</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">critical</tok> <tok cat="NN">hormone</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
863946106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">At</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">level</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="NN">HIV</tok> <tok cat="NN">infection</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">cause</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">apoptotic</tok> <tok cat="NN">cell</tok> <tok cat="NN">death</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">thymic</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
863946107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i2" role="SBJ"><tok cat="RB">chronically</tok> <tok cat="JJ">HIV-infected</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">appear</tok> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">resistant</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">glucocorticoid-evoked</tok> <tok cat="NN">cell</tok> <tok cat="NN">death</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
863946108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Glucocorticoid</tok> <tok cat="NN">receptor-ligand</tok> <tok cat="NN">binding</tok> <tok cat="NNS">studies</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NNS">patients</tok><tok cat="POS">'</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">reduced</tok> <tok cat="NN">affinity</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">receptor</tok> <tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">test</tok> <tok cat="NNS">steroids</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
863946109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="FW">In</tok> <tok cat="FW">vitro</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="RB">chronically</tok> <tok cat="JJ">HIV-infected</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">lymphoid</tok> <tok cat="NN">CEM</tok> <tok cat="NN">line</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">displayed</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">resistance</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">glucocorticoid-induced</tok> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
863946110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">reduced</tok> <tok cat="NNS">numbers</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBG">binding</tok> <tok cat="NNS">sites</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">little</tok> <tok cat="NN">alteration</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">apparent</tok> <tok cat="NN">affinity</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ligand</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">receptor</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
863946111@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="ADVP"><tok cat="RB">often</tok></cons> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">malfunction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">normal</tok> <tok cat="NN">glucocorticoid</tok> <tok cat="NN">response</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">HIV-infected</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">probably</tok></cons> <cons cat="ADJP"><tok cat="JJ">due</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">altered</tok> <tok cat="NNS">interactions</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">hormone</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
863946112@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Such</tok> <tok cat="NNS">alterations</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">clinical</tok> <tok cat="NNS">consequences</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">possibility</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">relatively</tok> <tok cat="JJR">longer</tok></cons> <tok cat="NN">life</tok> <tok cat="NN">span</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">infected</tok> <tok cat="JJ">CD4+</tok> <tok cat="NNS">T-lymphocytes</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">systemic</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">chronically</tok> <tok cat="JJ">elevated</tok></cons> <tok cat="NN">cortisol</tok> <tok cat="NN">level</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
864134601@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Interferon-gamma</tok></cons> <cons cat="VP"><tok cat="VBZ">modulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">lipopolysaccharide-induced</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">mRNA</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">level</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@16-12-2010
864134602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Interferon-gamma</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">modulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">several</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">level</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@16-12-2010
864134603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">view</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">findings</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">analyzed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">different</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
864134604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Priming</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">down</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-fos</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-jun</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lipopolysaccharide</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LPS</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="VBN">compared</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">LPS</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
864134605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="SINV"><cons cat="CONJP"><tok cat="RB">Not</tok> <tok cat="RB">only</tok></cons> <tok cat="VBD">was</tok> <cons cat="NP" id="i9" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">effect</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">level</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">activator</tok> <tok cat="NN">protein-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AP-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">capacity</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">affected</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">as</tok> <tok cat="RB">well</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">,</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">A</tok> <tok cat="JJ">strong</tok> <tok cat="NN">reduction</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">LPS-induced</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
864134606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">LPS-stimulated</tok> <tok cat="NNS">monocytes</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="VBN">increased</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">p105</tok> <tok cat="NN">mRNA</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">precursor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">p50</tok> <tok cat="NN">subunit</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="DT">no</tok> <tok cat="NN">effect</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">noticed</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p65</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
864134607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons> <tok cat="NN">priming</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p105</tok> <tok cat="NNS">transcripts</tok></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">enhanced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p65</tok> <tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" func="ADV"><tok cat="RB">two-fold</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
864134608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="NOM" role="SBJ"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Priming</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">followed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">LPS</tok> <tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">further</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p65</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
864134609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">due</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">half-life</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p65</tok> <tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">75</tok> <cons cat="NP" ref="i27" null="RNR"></cons></cons><tok cat="CC">vs</tok> <cons cat="NP"><tok cat="CD">150</tok> <cons cat="NP" ref="i27" null="RNR"></cons></cons><cons cat="NP" id="i27"><tok cat="NNS">minutes</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
864134610@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="VB">shift</tok> <tok cat="NNS">assays</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">EMSAs</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">unstimulated</tok> <tok cat="NNS">monocytes</tok></cons> <cons cat="ADVP"><tok cat="RB">predominantly</tok></cons> <cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">p50</tok></cons> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
864134611@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">LPS</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">p50</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p65</tok> <tok cat="NNS">subunits</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons> <tok cat="CC">plus</tok> <cons cat="NP"><tok cat="NN">LPS</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">further</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
864134612@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">With</tok> <cons cat="NP"><tok cat="NN">Western</tok> <tok cat="NN">blotting</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">p50</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p65</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">LPS</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons> <tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
864134613@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">combined</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">result</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="VBN">enhanced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">p50</tok></cons> <cons cat="NP" null="QSTN"></cons><cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">p65</tok> <tok cat="NN">protein</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NN">expression</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
864134614@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">specific</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">since</tok> <cons cat="S"><cons cat="NP" id="i13" role="SBJ"><tok cat="DT">no</tok> <tok cat="NN">change</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-IL-6</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
864134615@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">modulatory</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">cytokine</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
864135301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-fos</tok> <tok cat="CC">and</tok> <tok cat="NN">c-jun</tok> <tok cat="NNS">proteins</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">cycle</tok> <tok cat="NN">progression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HL60</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">phytohemagglutinin-stimulated</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@16-12-2010
864135302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">protein</tok> <tok cat="NNS">products</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">c-fos</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">p62c-fos</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">c-jun</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">p39c-jun</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">genes</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">family</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">thought</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="JJ">important</tok> <tok cat="NNS">roles</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@41@@oe@16-12-2010
864135303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="JJS">Most</tok> <tok cat="NNS">studies</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">relation</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">performed</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">level</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons> <cons cat="VP"><tok cat="VB">do</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">give</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">direct</tok> <tok cat="NN">information</tok></cons> <cons cat="PP"><tok cat="IN">about</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
864135304@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i34" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP"><tok cat="NN">Western</tok> <tok cat="NN">blotting</tok></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i34" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">investigate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NNS">cycles</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">different</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NNS">systems</tok></cons><cons cat="NP" ref="i30" null="ICH"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD" id="i30"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="RB">continuously</tok> <tok cat="VBG">growing</tok> <tok cat="JJ">myeloid</tok> <tok cat="JJ">leukemic</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">HL60</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="NN">cycle</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phyto- hemagglutinin</tok> <tok cat="LRB">(</tok><tok cat="NN">PHA</tok><tok cat="RRB">)</tok><tok cat="JJ">-stimulated</tok> <tok cat="JJ">normal</tok> <tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PBL</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@53@@oe@16-12-2010
864135305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i26" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">consists</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">dimers</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Fos</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Jun</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">family</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">studied</tok> <cons cat="NP" ref="i26" null="NONE"></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">gel</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
864135306@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p62c-fos</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p39c-jun</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">throughout</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok></cons> <cons cat="PP" syn="COOD"><tok cat="CC">both</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">HL60</tok> <tok cat="NNS">cells</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">PHA-stimulated</tok> <tok cat="NN">PBL</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">postulate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP" adv="TMP"><tok cat="IN">throughout</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok></cons></cons> <tok cat="CC">and</tok> <tok cat="RB">not</tok> <cons cat="ADVP"><tok cat="RB">transiently</tok></cons> <cons cat="PP"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">G0</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">G1</tok></cons></cons></cons> <tok cat="NN">transition</tok></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">previous</tok> <tok cat="NN">mRNA</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">indicated</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@52@@oe@16-12-2010
864135307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="UCP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">uncoupling</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">p62c-fos</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="COMP" null="ZERO"></cons><cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="NN">p39c-jun</tok></cons> <cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="ADVP"><cons cat="ADVP"><tok cat="RBR">more</tok> <tok cat="RB">strongly</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">G1-</tok> <cons cat="NP" null="QSTN"></cons><cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">G2/M-phase</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="JJ">enriched</tok> <tok cat="NNS">samples</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">S-phase</tok> <tok cat="JJ">enriched</tok> <tok cat="NNS">samples</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HL60</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">nuclear</tok> <cons cat="NP"><tok cat="NN">p62c-fos</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p39c-jun</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">constant</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@16-12-2010
864135308@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Nuclei</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">unstimulated</tok> <tok cat="NN">PBL</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">donors</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p62c-fos</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p39c-jun</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" id="i14" role="SBJ"><tok cat="NN">AP-1</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i14" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">majority</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">samples</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
864135309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="VBG">Following</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">PHA</tok></cons> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PBL</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i15" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">delayed</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">respect</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">augmentation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">p39c-jun</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
864135310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="JJ">cytoplasmic</tok> <cons cat="NP"><tok cat="NN">p62c-fos</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p39c-jun</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HL60</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">PHA-stimulated</tok> <tok cat="NN">PBL</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
864135311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i16" role="SBJ"><tok cat="DT">no</tok> <cons cat="NP"><tok cat="JJ">cytoplasmic</tok> <tok cat="NN">p62c-fos</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i16" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">unstimulated</tok> <tok cat="NN">PBL</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">although</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">some</tok> <tok cat="NNS">cases</tok></cons></cons> <cons cat="NP" id="i18" role="SBJ"><tok cat="JJ">cytoplasmic</tok> <tok cat="NN">p39c-jun</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok><cons cat="NP" ref="i18" null="NONE"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">subcellular</tok> <tok cat="NN">compartmentalization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NNS">proteins</tok></cons></cons></cons></cons><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">occur</tok> <cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><tok cat="JJ">certain</tok> <tok cat="NNS">circumstances</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
864146701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">Ca2+</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">oxidant-induced</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activation</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@10@@oe@16-12-2010
864146702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">subclone</tok></cons> <cons cat="NP"><tok cat="NN">Wurzburg</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="NP" id="i5" role="SBJ"><tok cat="NN">oxidant</tok> <tok cat="NN">challenge</tok></cons> <cons cat="VP"><tok cat="VBD">elevated</tok> <cons cat="NP"><tok cat="NN">[Ca2+]i</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">mobilizing</tok> <cons cat="NP"><tok cat="NN">Ca2+</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NNS">stores</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@16-12-2010
864146703@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">effect</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="JJ">rapid</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">transient</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Wurzburg</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">response</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="JJ">slow</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">sustained</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
864146704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="JJ">H2O2-induced</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Wurzburg</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">influenced</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">extracellular</tok> <tok cat="NN">EGTA</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">totally</tok></cons> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" id="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">loaded</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="VBN">esterified</tok> <tok cat="NN">EGTA</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
864146705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">cells</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">sensitive</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">H2O2-induced</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">H2O2</tok></cons> <cons cat="VP"><tok cat="VBD">potentiated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">sustained</tok> <tok cat="JJ">high</tok></cons> <tok cat="NN">[Ca2+]i</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="VBG">following</tok> <cons cat="NP"><tok cat="NN">thapsigargin</tok> <tok cat="NN">treatment</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
864146706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <tok cat="JJ">regulatory</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">alpha-lipoate</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">N-acetylcysteine</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">appeared</tok> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">part</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">due</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">stabilize</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">elevation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">[Ca2+]i</tok></cons></cons></cons> <cons cat="PP"><tok cat="VBG">following</tok> <cons cat="NP"><tok cat="NN">oxidant</tok> <tok cat="NN">challenge</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
864146707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Results</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="JJ">sustained</tok> <tok cat="JJ">elevated</tok></cons> <tok cat="NN">[Ca2+]i</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">significant</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">oxidant-induced</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
864180501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">Effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">interleukin-10</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NN">cell</tok> <tok cat="NNS">responses</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Cryptococcus</tok> <tok cat="NNS">neoformans</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Candida</tok> <tok cat="NNS">albicans</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">lipopolysaccharide</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
864180502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Deactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NNS">phagocytes</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">critical</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">limit</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inflammatory</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">detrimental</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">face</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">progressive</tok> <tok cat="NN">infection</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
864180503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">compared</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBG">deactivating</tok> <tok cat="NN">cytokine</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="CD">10</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-10</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NN">cell</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PBMC</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">responses</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">lipopolysaccharide</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LPS</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Cryptococcus</tok> <tok cat="NNS">neoformans</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Candida</tok> <tok cat="NNS">albicans</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
864180504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-10</tok></cons> <cons cat="VP"><tok cat="VBD">effected</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">dose-dependent</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">release</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PBMC</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">LPS</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="FW">C.</tok> <tok cat="FW">neoformans</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">with</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i57" role="SBJ"><tok cat="JJ">significant</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="VP"><tok cat="VBN">seen</tok> <cons cat="NP" ref="i57" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="CD">0.1</tok> <tok cat="NN">U/ml</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i58" role="SBJ"><cons cat="NP"><cons cat="QP"><tok cat="JJR">greater</tok> <tok cat="IN">than</tok> <tok cat="CD">90</tok></cons><tok cat="NN">%</tok></cons> <cons cat="NP"><tok cat="NN">inhibition</tok></cons></cons> <cons cat="VP"><tok cat="VBN">noted</tok> <cons cat="NP" ref="i58" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="CD">10</tok> <tok cat="NN">U/ml</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
864180505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="RB">even</tok> <tok cat="IN">at</tok> <cons cat="NP"><tok cat="NNS">doses</tok> <cons cat="NP"><cons cat="QP"><tok cat="RB">as</tok> <tok cat="JJ">high</tok> <tok cat="IN">as</tok> <tok cat="CD">100</tok></cons> <tok cat="NN">U/ml</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">IL-10</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">release</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="FW">C.</tok> <tok cat="FW">albicans</tok></cons></cons> <cons cat="NP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="QP"><tok cat="RB">only</tok> <tok cat="CD">50</tok></cons><tok cat="NN">%</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
864180506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-10</tok></cons> <cons cat="ADVP"><tok cat="RB">profoundly</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">release</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-1beta</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PBMC</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="QP"><tok cat="DT">all</tok> <tok cat="CD">three</tok></cons> <tok cat="NNS">stimuli</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
864180507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i59" role="SBJ"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">mRNA</tok> <tok cat="CC">and</tok> <tok cat="NN">release</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i59" null="NONE"></cons><cons cat="SBAR"><tok cat="RB">even</tok> <tok cat="IN">if</tok> <cons cat="S"><cons cat="NP" id="i60" role="SBJ"><tok cat="NN">IL-10</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">added</tok> <cons cat="NP" ref="i60" null="NONE"></cons><cons cat="PP" adv="TMP"><cons cat="NP"><cons cat="QP"><tok cat="RP">up</tok> <tok cat="TO">to</tok> <tok cat="CD">8</tok></cons> <tok cat="NN">h</tok></cons> <tok cat="IN">after</tok> <cons cat="NP"><tok cat="JJ">cryptococcal</tok> <tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
864180508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="NN">beta</tok> <tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="PP" role="PRD"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJR">lesser</tok> <tok cat="NN">magnitude</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">occurred</tok> <cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i61"><tok cat="RB">only</tok> <tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i62" role="SBJ"><tok cat="NN">IL-10</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">added</tok> <cons cat="NP" ref="i62" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">2</tok> <tok cat="NN">h</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">cryptococcal</tok> <tok cat="NN">stimulation</tok></cons></cons></cons></cons><cons cat="ADVP" ref="i61" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
864180509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-10</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">LPS</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">fungal</tok> <tok cat="NNS">stimuli</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
864180510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">All</tok> <tok cat="CD">three</tok> <tok cat="NNS">stimuli</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-10</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">PBMC</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" id="i63" role="SBJ"><cons cat="QP"><tok cat="IN">over</tok> <tok cat="RB">10-fold</tok> <tok cat="JJR">less</tok></cons> <tok cat="NN">IL-10</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">released</tok> <cons cat="NP" ref="i63" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="FW">C.</tok> <tok cat="FW">neoformans</tok></cons></cons> <cons cat="PP"><tok cat="VBN">compared</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">LPS</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">C.</tok> <tok cat="NNS">albicans</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
864180511@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-10</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><tok cat="VBG">deactivating</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PBMC</tok> <tok cat="NNS">responses</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="QP"><tok cat="DT">all</tok> <tok cat="CD">three</tok></cons> <tok cat="NNS">stimuli</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i64" role="SBJ"><cons cat="NP"><tok cat="JJ">disparate</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><cons cat="NP"><tok cat="NN">stimulus-</tok></cons> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">response-specific</tok></cons></cons> <tok cat="NNS">patterns</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">deactivation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">seen</tok><cons cat="NP" ref="i64" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
864180512@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i65" role="SBJ"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">proinflammatory</tok> <tok cat="NN">cytokine</tok> <tok cat="NN">release</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="S"><cons cat="NP" ref="i65" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">occur</tok> <cons cat="PP" id="i66" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons> <cons cat="PP" id="i67"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons map="i66" cat="ADVP" syn="COOD" adv="TMP"><tok cat="CC">both</tok> <cons cat="ADVP"><tok cat="RB">transcriptionally</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">posttranscriptionally</tok></cons></cons> <cons map="i67" cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">IL-1beta</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
864226801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HLA-DQB1</tok> <tok cat="NN">codon</tok> <tok cat="CD">57</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">critical</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><tok cat="NN">peptide</tok> <cons cat="NP"><tok cat="NN">binding</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">recognition</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@11@@oe@16-12-2010
864226802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">association</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <cons cat="NP"><tok cat="NN">HLA-DQ</tok> <tok cat="NNS">alleles</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">autoimmunity</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">correlated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">discrete</tok> <tok cat="NNS">polymorphisms</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HLA-DQ</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" id="i15" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">localized</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">sites</tok></cons> <cons cat="ADJP"><tok cat="JJ">suitable</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">peptide</tok> <tok cat="NN">recognition</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@16-12-2010
864226803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">polymorphism</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">residue</tok> <tok cat="CD">57</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DQB1</tok> <tok cat="NN">polypeptide</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="PP" role="PRD"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">particular</tok> <tok cat="NN">interest</tok></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">since</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">major</tok> <tok cat="JJ">structural</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">formation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">salt</tok> <tok cat="NN">bridge</tok> <tok cat="NN">structure</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok> <tok cat="NN">end</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">peptide-binding</tok> <tok cat="NN">cleft</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DQ</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
864226804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">This</tok> <tok cat="NN">polymorphism</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="NN">residue</tok> <tok cat="CD">57</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">recurrent</tok> <tok cat="NN">feature</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HLA-DQ</tok> <tok cat="NN">evolution</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">occurring</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">multiple</tok> <tok cat="JJ">distinct</tok> <tok cat="JJ">allelic</tok></cons> <tok cat="NNS">families</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i14"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i14" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">implies</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">functional</tok> <tok cat="NN">selection</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">maintaining</tok> <cons cat="NP"><tok cat="NN">variation</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">position</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">molecule</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
864226805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">directly</tok></cons> <cons cat="VP"><tok cat="VBD">tested</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">amino</tok> <tok cat="NN">acid</tok> <tok cat="NN">polymorphism</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">site</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">determinant</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">peptide</tok> <tok cat="NN">binding</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">antigen-specific</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
864226806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">single</tok> <tok cat="NN">Ala</tok><tok cat="TO">--&gt;</tok><tok cat="NN">Asp</tok> <tok cat="NN">amino</tok> <tok cat="NN">acid</tok> <tok cat="CD">57</tok> <tok cat="NN">substitution</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">HLA-DQ3.2</tok> <tok cat="NN">molecule</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">regulated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">HSV-2</tok> <tok cat="JJ">VP-16-derived</tok> <tok cat="NN">peptide</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
864226807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">complementary</tok> <tok cat="NN">single-residue</tok> <tok cat="NN">substitution</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">peptide</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">abolished</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">DQ3.2</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">converted</tok> <cons cat="NP"><tok cat="PRP">it</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">peptide</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">DQ3.1</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">DQ3.3</tok> <tok cat="JJ">Asp-57-positive</tok> <tok cat="NN">MHC</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
864226808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">These</tok> <tok cat="NN">binding</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">paralleled</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">recognition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">class</tok> <tok cat="NN">II-peptide</tok> <tok cat="NN">complex</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHPP" id="i6"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="VBN">substituted</tok> <tok cat="NN">peptide</tok></cons> <cons cat="VP"><tok cat="VBD">abolished</tok> <cons cat="PP" ref="i6" null="T"></cons><cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">reactivity</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" id="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">directed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">DQ3.2-peptide</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">clone</tok></cons></cons> <cons cat="VP"><tok cat="VBD">recognized</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">substituted</tok> <tok cat="NN">peptide</tok></cons> <cons cat="VP"><tok cat="VBN">presented</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">DQ3.3</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">restriction</tok> <tok cat="NN">element</tok></cons> <cons cat="VP"><tok cat="VBG">differing</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">DQ3.2</tok></cons></cons> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <tok cat="IN">at</tok> <cons cat="NP"><tok cat="NN">residue</tok> <tok cat="CD">57</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@61@@oe@16-12-2010
864226809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">This</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">structural</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">functional</tok></cons></cons> <tok cat="NN">complementarity</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">residue</tok> <tok cat="CD">57</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">specific</tok> <tok cat="NN">peptide</tok> <tok cat="NN">residue</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">identifies</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">key</tok> <tok cat="VBG">controlling</tok> <tok cat="NN">determinant</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">restricted</tok> <tok cat="NN">recognition</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">HLA-DQ-specific</tok> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
864228201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">A</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="JJ">type-specific</tok> <tok cat="NN">enhancer</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B7.1</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
864228202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">costimulatory</tok> <tok cat="NN">molecule</tok> <tok cat="NN">B7.1</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">provides</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">second</tok> <tok cat="NN">signal</tok></cons> <cons cat="ADJP"><tok cat="JJ">critical</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@16-12-2010
864228203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">distribution</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <cons cat="NP"><tok cat="JJ">integral</tok> <tok cat="NN">membrane</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">restricted</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">certain</tok> <tok cat="NNS">tissues</tok></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i1"><tok cat="WRB">where</tok></cons> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">modulated</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">multiple</tok></cons> <cons cat="ADJP"><tok cat="JJ">exogenous</tok></cons></cons> <tok cat="NNS">stimuli</tok></cons></cons><cons cat="ADVP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
864228204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">identify</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">basis</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">specificity</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">inducibility</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">chromatin</tok></cons> <tok cat="NN">configuration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B7.1</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">intact</tok> <tok cat="NNS">nuclei</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
864228205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">identification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">tissue-specific</tok> <cons cat="NP"><tok cat="NN">deoxyribonuclease</tok> <tok cat="CD">I</tok> <tok cat="JJ">hypersensitive</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">approximately</tok> <cons cat="NP"><tok cat="JJ">3kb</tok> <tok cat="JJ">upstream</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">start</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">led</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">characterization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="JJ">type-specific</tok> <tok cat="NN">enhancer</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
864228206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <cons cat="NP"><tok cat="JJ">183-bp</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="CC">both</tok> <cons cat="ADJP"><tok cat="NN">cell</tok> <tok cat="NN">type</tok> <tok cat="JJ">specific</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">responsive</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">distinct</tok> <tok cat="NNS">stimuli</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">lipopolysaccharide</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">dibutyryl</tok> <tok cat="NN">cAMP</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">B7.1</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
864228207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">Deletional</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">site-directed</tok></cons></cons> <tok cat="NN">mutagenesis</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">multiple</tok> <tok cat="RB">functionally</tok> <tok cat="JJ">critical</tok></cons> <cons cat="NP"><tok cat="NN">cis</tok> <tok cat="NNS">elements</tok></cons></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">region</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i6"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="WHPP"><tok cat="IN">of</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="LRB">(</tok><tok cat="NN">NF</tok><tok cat="RRB">)</tok><tok cat="NN">-kappaB</tok> <tok cat="NN">consensus</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
864228208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">B7.1-positive</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">element</tok></cons> <cons cat="VP"><tok cat="VBD">bound</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">family</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">already</tok></cons> <tok cat="VBN">implicated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">transduction</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="ADJP"><tok cat="JJ">relevant</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">B7.1</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
864228209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NN">description</tok><cons cat="PP" ref="i16" null="ICH"></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="PRPP">our</tok> <tok cat="NN">knowledge</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP" id="i16"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">regulatory</tok> <tok cat="NNS">elements</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">control</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">gene</tok></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">B7</tok> <tok cat="JJ">costimulatory</tok> <tok cat="NN">molecule</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
864268601@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">Epstein-Barr</tok> <tok cat="JJ">virus-encoded</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">antigen</tok> <tok cat="NN">EBNA-5</tok></cons> <cons cat="VP"><tok cat="VBZ">accumulates</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">PML-containing</tok> <tok cat="NNS">bodies</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
864268602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">EBNA-5</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok> <tok cat="LRB">(</tok><tok cat="NN">EBV</tok><tok cat="RRB">)</tok><tok cat="JJ">-encoded</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">immortalization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
864268603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">nuclei</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">EBV-transformed</tok> <tok cat="JJ">lymphoblastoid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons> <cons cat="NP" id="i1" role="SBJ"><tok cat="NN">EBNA-5</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">preferentially</tok></cons> <cons cat="VP"><tok cat="VBN">targetted</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">distinct</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">foci</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
864268604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Previously</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNP">W.Q.</tok> <tok cat="NNP">Jiang</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">L.Szekely</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">V.Wendel-Hansen</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">N.Ringertz</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">G.Klein</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNP">A.</tok> <tok cat="NNP">Rosen</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Exp.Cell</tok> <tok cat="NNP">Res</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
864508601@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Heat</tok> <tok cat="NN">shock</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">replication</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">chronically</tok> <tok cat="JJ">infected</tok></cons> <cons cat="NP"><tok cat="JJ">promyelocyte</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">OM10.1</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
864508602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">long</tok> <tok cat="NN">period</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">clinical</tok> <tok cat="NN">latency</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">before</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">development</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">symptoms</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">characteristic</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HIV-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">infection</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@16-12-2010
864508603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">OM10.1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">promyelocyte</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">latently</tok></cons> <tok cat="VBN">infected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">developed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">model</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">studying</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">latency</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">virus</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
864508604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">this</tok> <cons cat="ADJP"><tok cat="RB">latently</tok> <tok cat="JJ">infected</tok></cons> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">heat</tok> <tok cat="NN">shock</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="CD">42</tok> <tok cat="NNS">degrees</tok> <tok cat="NN">C</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="CD">2</tok> <tok cat="NN">h</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">high</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">production</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">any</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
864508605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">activation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">anti-TNF-alpha</tok> <tok cat="NN">antibody</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NNS">inhibitors</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
864508606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">culture</tok> <tok cat="NNS">supernatants</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="PP" role="PRD"><tok cat="IN">below</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">sensitivity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">ELISA</tok> <tok cat="NN">assay</tok> <tok cat="NN">system</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">anti-TNF-alpha</tok> <tok cat="NN">antibody</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">culture</tok> <tok cat="NN">medium</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="ADVP"><tok cat="RB">partially</tok></cons> <cons cat="VP"><tok cat="VB">suppress</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">heat</tok> <tok cat="NN">shock</tok> <tok cat="JJ">induced</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">production</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
864508607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Staurosporine</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PKC</tok> <tok cat="NN">inhibitor</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">pentoxifylline</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">inhibitor</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Ro5-3335</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HIV-1</tok> <cons cat="NP"><tok cat="NN">Tat</tok></cons> <tok cat="NN">inhibitor</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">heat</tok> <tok cat="NN">shock</tok> <tok cat="JJ">induced</tok> <tok cat="NN">virus</tok> <tok cat="NN">activation</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
864508608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">particular</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">staurosporine</tok></cons> <cons cat="VP"><tok cat="VBD">achieved</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><cons cat="QP"><tok cat="RB">approximately</tok> <tok cat="CD">90</tok></cons><tok cat="NN">%</tok></cons> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">antigen</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">heat</tok> <tok cat="JJ">shock-treated</tok> <tok cat="NN">OM10.1</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">non-toxic</tok> <tok cat="NN">concentration</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
864508609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">heat</tok> <tok cat="NN">shock</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="ADVP"><tok cat="RB">fully</tok></cons> <cons cat="VP"><tok cat="VBN">elucidated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="ADVP" adv="TMP"><tok cat="RB">yet</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">presumed</tok> <cons cat="COMP" null="ZERO"></cons><cons cat="NP" role="SBJ"><tok cat="NN">PKC</tok></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
864508610@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NNS">observations</tok></cons> <cons cat="VP"><tok cat="MD">will</tok> <cons cat="VP"><tok cat="VB">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">further</tok> <tok cat="NN">insight</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathogenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NNS">infections</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
864525401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Abundant</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">P45</tok> <tok cat="NN">NF-E2</tok> <tok cat="NN">mRNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NNS">granurocytes</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@16-12-2010
864525402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">NF-E2</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">crucial</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">erythroid-specific</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@12@@oe@16-12-2010
864525403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i15" role="SBJ"><cons cat="NP"><tok cat="NN">p45</tok> <tok cat="NN">subunit</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-E2</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">basic-leucine</tok> <tok cat="NN">zipper</tok> <tok cat="NN">domain</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">dimerizes</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">small</tok> <tok cat="NN">Maf</tok> <tok cat="NN">family</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i15" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">form</tok> <cons cat="NP"><tok cat="JJ">functional</tok> <cons cat="NP"><tok cat="NN">NF-E2</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
864525404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">While</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">p45</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">restricted</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNS">megakaryocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">mast</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NN">lineage</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="NN">p45</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">abundantly</tok></cons> <cons cat="VP"><tok cat="VBN">transcribed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">granulocyte</tok> <tok cat="NN">fraction</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
864525405@GENIA Treebank@formal@@1@S@<cons cat="FRAG"><cons cat="SBAR" func="ADV"><tok cat="IN">As</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">neutrophils</tok></cons> <cons cat="VP"><tok cat="VBP">occupy</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="RB">approximately</tok> <tok cat="CD">92</tok></cons><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">granulocyte</tok> <tok cat="NN">fraction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
864525406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">As</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">neutrophils</tok></cons> <cons cat="VP"><tok cat="VBP">occupy</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="RB">approximately</tok> <tok cat="CD">92</tok></cons><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">fraction</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP"><tok cat="NN">p45</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="RBS">most</tok> <tok cat="JJ">likely</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><tok cat="NNS">neutrophils</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
864525407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="NN">p45</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">HL-60</tok> <tok cat="NNS">promyelocytes</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">albeit</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">expression</tok> <tok cat="NN">level</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="RB">much</tok> <tok cat="JJR">lower</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">granulocyte</tok> <tok cat="NN">fraction</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
864525408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="NN">HL-60</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">express</tok> <cons cat="NP"><tok cat="NN">mafK</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">genuine</tok> <cons cat="NP"><tok cat="NN">NF-E2</tok> <tok cat="NN">complex</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
864525409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="NN">p45</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">transcribed</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">different</tok> <tok cat="NNS">promoters</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">aNF-E2</tok> <tok cat="NN">promoter</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">fNF-E2</tok> <tok cat="NN">promoter</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">erythroid</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">megakaryocytic</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">lineage</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i13" role="SBJ"><tok cat="NN">p45</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">transcribed</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">aNF-E2</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
864525410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">p45</tok></cons> <cons cat="NP"><tok cat="JJ">megakaryocytic</tok> <tok cat="NN">lineage</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p45</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">transcribed</tok> <cons cat="NP" ref="i14" null="NONE"></cons><cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">aNF-E2</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
864525411@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p45</tok> <tok cat="NN">mRNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NNS">neutrophils</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">declined</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transfer</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">culture</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">G-CSF</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">sustain</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">down-regulation</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="COMP" null="ZERO"></cons><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">E2</tok></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VB">participate</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">neutrophil-specific</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
864530301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Retinoid</tok> <tok cat="NN">X</tok> <tok cat="NN">Receptor</tok> <tok cat="NN">alpha</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">increased</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NN">cell</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
864530302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">1</tok> <tok cat="NN">alpha, 25-Dihydroxyvitamin</tok> <tok cat="NN">D3</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">VD</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potent</tok> <tok cat="NN">inducer</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">normal</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">leukemic</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@16-12-2010
864530303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="PRPP">Its</tok> <tok cat="NNS">effects</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="PRPP">its</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">receptor</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">VDR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
864530304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Efficient</tok> <tok cat="NN">gene</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">heterodimerization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">VDR</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Retinoid</tok> <tok cat="NN">X</tok> <tok cat="NNS">Receptors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RXR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
864530305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NNS">antibodies</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">analyzed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">RXR</tok> <tok cat="NN">alpha</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">myeloid</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AML</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">10</tok> <tok cat="NNS">cases</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">myelomonocytic</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="VP"><tok cat="VBN">arrested</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">stages</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
864530306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">RXR</tok> <tok cat="NN">alpha</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">myelomonocytic</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">since</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJS">highest</tok> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">AML</tok> <tok cat="NNS">samples</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">myelomonocytic</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="VP"><tok cat="VBG">having</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJS">highest</tok> <tok cat="NNS">amounts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NNS">precursors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
864530307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">fresh</tok> <cons cat="NP"><tok cat="JJ">leukemic</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><cons cat="WHNP"><tok cat="WDT">whatever</tok> <cons cat="FRAG"><cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">stage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><tok cat="JJ">myelomonocytic</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VB">respond</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">VD</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">RXR</tok> <tok cat="NN">alpha</tok></cons> <tok cat="NNS">levels</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
864530308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Combinations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">all-trans</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">VD</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">some</tok> <tok cat="NNS">cases</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">effect</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
864530309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">response</tok></cons> <cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">RXR</tok> <tok cat="NN">alpha</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NN">differentiation</tok></cons></cons> <cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><tok cat="NN">VD</tok> <tok cat="NN">treatment</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
864561601@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Putative</tok> <tok cat="NN">steroid</tok> <tok cat="NN">binding</tok> <tok cat="NN">domain</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">mineralocorticoid</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="FW">E.</tok> <tok cat="FW">coli</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">heat</tok> <tok cat="NN">shock</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">shows</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">typical</tok></cons> <cons cat="ADJP"><tok cat="JJ">native</tok></cons></cons> <tok cat="NN">receptor</tok> <tok cat="NNS">characteristics</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@16-12-2010
864561602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">Domain</tok> <tok cat="NN">E</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">considered</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">putative</tok> <tok cat="NN">hormone</tok> <tok cat="NN">binding</tok> <tok cat="NN">domain</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HBD</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">mineralocorticoid</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">hMR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="FW">Escherichia</tok> <tok cat="FW">coli</tok></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">maltose</tok> <tok cat="NN">binding</tok> <tok cat="NN">protein</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MBP</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glutathione</tok> <tok cat="NN">S-transferase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GST</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@45@@oe@16-12-2010
864561603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <cons cat="NP"><tok cat="JJ">bacterially-produced</tok> <tok cat="NN">MR</tok> <tok cat="NNS">constructs</tok></cons></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">steroid</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="ADVP"><tok cat="FW">per</tok> <tok cat="FW">se</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
864561604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">fact</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">heat</tok> <tok cat="NN">shock</tok> <tok cat="NN">protein</tok></cons> <tok cat="NN">association</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">hsp</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NN">affinity</tok> <tok cat="NN">ligand-binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">MR</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
864561605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">After</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">incubation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="VBN">purified</tok> <cons cat="NP"><tok cat="NN">MBP-</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">GST-HBD</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP" id="i8"><tok cat="NN">rabbit</tok> <tok cat="NN">reticulocyte</tok> <tok cat="NN">lysate</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">rich</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">heat</tok> <tok cat="NN">shock</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">obtained</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">saturable</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">[3H]aldosterone</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
864561606@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">Kd</tok> <tok cat="NN">value</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">aldosterone</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><tok cat="CD">0.3</tok> <tok cat="NN">nM</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">Bmax</tok></cons> <cons cat="VP"><tok cat="VBD">=</tok> <cons cat="NP"><tok cat="CD">32</tok> <tok cat="NN">pmol/mg</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
864561607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="NN">Hormone</tok> <tok cat="NN">binding</tok> <tok cat="NN">specificity</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">assessed</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">competition</tok> <tok cat="NNS">studies</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NN">steroid</tok> <tok cat="NNS">ligands</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
864561608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Sucrose</tok> <tok cat="NN">gradient</tok> <tok cat="NNS">assays</tok></cons> <cons cat="VP"><tok cat="VBN">performed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">[3H]aldosterone-MBP-HBD</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">complex</tok></cons> <cons cat="VP"><tok cat="VBG">sedimenting</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">8.3S</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">4.9S</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">[3H]progesterone-MBP-HBD</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
864561609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Western-blot</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">sedimentation</tok> <tok cat="NN">peak</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">concomitant</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" id="i14"><tok cat="NN">MBP-HBD</tok></cons></cons> <cons cat="PP" id="i41"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">monoclonal</tok> <tok cat="JJ">anti-MBP</tok> <tok cat="NN">antibody</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="PP"><cons map="i14" cat="NP"><tok cat="NN">hsp90</tok></cons></cons> <cons map="i41" cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">monoclonal</tok> <tok cat="JJ">anti-hsp</tok> <tok cat="NN">antibody</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
864561610@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">following</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">incubation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">anti-rabbit</tok> <tok cat="NN">hsp90</tok> <tok cat="JJ">monoclonal</tok> <tok cat="NN">antibody</tok></cons></cons></cons></cons></cons> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">sedimenting</tok> <tok cat="NN">gradient</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">10.4S</tok> <tok cat="NN">sedimenting</tok> <tok cat="NN">complex</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
864561611@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">analyses</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">[3H]aldosterone-MBP-HBD</tok> <tok cat="NN">complex</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">hsp90</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">reticulocyte</tok> <tok cat="NN">lysate</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HBD</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">hMR</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">sufficient</tok></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="NP"><tok cat="NN">hsp90</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
864561612@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Deletions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">relatively</tok> <tok cat="JJ">short</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">amino-</tok> <cons cat="NP" null="QSTN"></cons></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">729-766</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" ref="i25" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">carboxy- terminal</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">940-984</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" ref="i25" null="RNR"></cons></cons><cons cat="NP" id="i25"><tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HBD</tok> <tok cat="NN">fragment</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">eliminated</tok> <cons cat="NP"><tok cat="DT">all</tok> <tok cat="JJ">steroid-binding</tok> <tok cat="NNS">properties</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
864561613@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Overall</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">integrity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">domain</tok> <tok cat="NN">E</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" syn="COOD" role="PRD"><cons cat="ADJP"><tok cat="JJ">necessary</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">sufficient</tok></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">steroid</tok> <tok cat="NNS">ligands</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">agonists</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NNS">antagonists</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">characteristics</tok></cons> <cons cat="ADJP"><tok cat="JJ">similar</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="JJ">entire</tok> <tok cat="JJ">native</tok></cons> <cons cat="NP"><tok cat="NN">MR</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
864977901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">p105</tok></cons> <tok cat="NN">processing</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">NF-kappaB</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="RB">transiently</tok> <tok cat="VBN">transfected</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@12@@oe@16-12-2010
864977902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">proteasome-mediated</tok> <tok cat="NN">processing</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-kappaB1</tok> <tok cat="NN">p105</tok> <tok cat="NN">precursor</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">generates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">p50</tok> <tok cat="NN">subunit</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@16-12-2010
864977903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">processing</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p105</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADVP"><tok cat="RB">markedly</tok></cons> <cons cat="VP"><tok cat="VBN">enhanced</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">various</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">activation</tok> <tok cat="NNS">agents</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="JJ">underlying</tok> <tok cat="JJ">regulatory</tok></cons> <tok cat="NN">mechanism</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">not</tok> <tok cat="RB">yet</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">clear</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
864977904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="JJ">signal-mediated</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">p105</tok></cons> <tok cat="NN">processing</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">de</tok> <tok cat="FW">novo</tok></cons> <tok cat="NN">synthesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <cons cat="NP"><tok cat="JJ">precursor</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
864977905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Transient</tok> <tok cat="NN">transfection</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBN">performed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">COS7</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">newly</tok> <tok cat="VBN">synthesized</tok></cons> <cons cat="NP"><tok cat="NN">p105</tok></cons> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADVP" adv="TMP"><tok cat="RBR">more</tok> <tok cat="RB">rapidly</tok></cons> <cons cat="VP"><tok cat="VBN">processed</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="VBN">compared</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="VBN">accumulated</tok> <tok cat="NN">form</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" id="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">already</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">processed</tok> <tok cat="NN">product</tok></cons> <cons cat="NP"><tok cat="NN">p50</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
864977906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Interestingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">processing</tok> <tok cat="NN">rate</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p105</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">markedly</tok></cons> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">co-transfected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p50</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NNS">subunits</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">RelA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Rel</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">physically</tok></cons> <cons cat="VP"><tok cat="VBP">interact</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">p105</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
864977907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">processing</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p105</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">subject</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">negative</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="JJ">various</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NNS">subunits</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
864977908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RBR">further</tok></cons> <cons cat="VP"><tok cat="VB">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">p105</tok></cons> <cons cat="VP"><tok cat="VBZ">undergoes</tok> <cons cat="NP"><tok cat="NN">degradation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">lipopolysaccharide-stimulated</tok> <tok cat="JJ">human</tok> <tok cat="JJ">monocytic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
864977909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inducible</tok> <tok cat="NN">degradation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p105</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">coupled</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">generation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p50</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
864977910@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Together</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">processing</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">inducible</tok> <tok cat="NN">degradation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p105</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">differentially</tok></cons> <cons cat="VP"><tok cat="VBN">regulated</tok><cons cat="NP" ref="i10" null="NONE"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
864982201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Identification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">LIM-Hox</tok> <tok cat="NN">gene</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">hLH-2</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="UCP" syn="COOD"><cons cat="VP"><cons cat="ADVP"><tok cat="RB">aberrantly</tok></cons> <tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">chronic</tok> <tok cat="JJ">myelogenous</tok> <tok cat="NN">leukaemia</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">9q33-34.1</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@16-12-2010
864982202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">describe</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">isolation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">LH-2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">putative</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">two</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cysteine-rich</tok> <tok cat="NNS">regions</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LIM</tok> <tok cat="NNS">domains</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">homeobox</tok> <tok cat="LRB">(</tok><tok cat="NN">Hox</tok><tok cat="RRB">)</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@29@@oe@16-12-2010
864982203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="JJ">High</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">hLH-2</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="NNS">cases</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">chronic</tok> <tok cat="JJ">myelogenous</tok> <tok cat="NN">leukaemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CML</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBN">tested</tok><cons cat="NP" null="NONE"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">regardless</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">disease</tok> <tok cat="NN">status</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
864982204@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="NN">hLH-2</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">mapped</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="NN">9Q33-34.1</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">reciprocal</tok> <tok cat="NN">translocation</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">creates</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">BCR-ABL</tok> <tok cat="NN">chimera</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Philadelphia</tok> <tok cat="NN">chromosome</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Ph</tok><tok cat="RQT">'</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">hallmark</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CML</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="NN">hLH-2</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">retained</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">derivative</tok> <tok cat="CD">9</tok> <tok cat="NN">chromosome</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="JJ">centromeric</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-ABL</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@49@@oe@16-12-2010
864982205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">proximity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">hLH-2</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">breakpoint</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="CD">9</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">raises</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">possibility</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cis-activation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">t(9;22)(q34;q11)</tok> <tok cat="NN">translocation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
864982206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">finding</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">hLH-2</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="NNS">cases</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CML</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">lymphoid</tok> <tok cat="NNS">malignancies</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NNS">cases</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">myelogenous</tok> <tok cat="NN">leukaemia</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
864982207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">hLH-2</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">development</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">progression</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">leukaemia</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">known</tok><cons cat="NP" ref="i6" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
864982208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">hLH-2</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">prove</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">useful</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">marker</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CML</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">monitoring</tok> <cons cat="NP"><tok cat="JJ">residual</tok> <tok cat="NN">disease</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
865284101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">BCL-6</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">activation</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@6@@oe@16-12-2010
865284102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Translocations</tok></cons> <cons cat="VP"><tok cat="VBG">involving</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">BCL-6</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">common</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">diffuse</tok></cons> <cons cat="ADJP"><tok cat="JJ">large</tok></cons></cons> <tok cat="NN">cell</tok> <tok cat="NN">subtype</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">non-Hodgkin</tok><tok cat="POS">'s</tok></cons></cons> <tok cat="NN">lymphoma</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@16-12-2010
865284103@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="ADVP"><tok cat="RB">Invariably</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">BCL-6</tok></cons> <tok cat="NN">coding</tok> <tok cat="NN">region</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">intact</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="PRPP">its</tok> <cons cat="NP"><tok cat="JJ">5'</tok> <tok cat="JJ">untranslated</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">replaced</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">sequences</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">translocation</tok> <tok cat="NN">partner</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
865284104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBZ">shows</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="NN">BCL-6</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">mitogenic</tok> <tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
865284105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="VBG">Resting</tok> <cons cat="NP"><tok cat="NN">B</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons><cons cat="NP" id="i3"><tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="VP"><tok cat="VBP">contain</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">BCL-6</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
865284106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">mouse</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">anti-IgM</tok> <cons cat="NP" ref="i4" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">IgD</tok> <cons cat="NP" ref="i4" null="RNR"></cons></cons><cons cat="NP" id="i4"><tok cat="NNS">antibodies</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">bacterial</tok> <tok cat="NN">lipopolysaccharide</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">12-myristate</tok> <tok cat="NN">13-acetate</tok></cons> <tok cat="CC">plus</tok> <cons cat="NP"><tok cat="NN">ionomycin</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">CD40</tok> <tok cat="NN">ligand</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">led</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="JJ">five-fold</tok> <tok cat="TO">to</tok> <tok cat="JJ">35-fold</tok></cons> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">BCL-6</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="NNS">levels</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
865284107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="JJ">Similar</tok> <tok cat="NN">downregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">BCL-6</tok> <tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">seen</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="FW">Staphylococcus</tok> <tok cat="FW">aureus</tok></cons> <tok cat="CC">plus</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">interleukin-2</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">anti-IgM</tok> <tok cat="NNS">antibodies</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">phytohemagglutinin</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
865284108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">BCL-6</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBD">began</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">decrease</tok> <cons cat="PP" adv="TMP"><cons cat="NP"><cons cat="QP"><tok cat="CD">8</tok> <tok cat="TO">to</tok> <tok cat="CD">16</tok></cons> <tok cat="NNS">hours</tok></cons> <tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV" adv="TMP"><tok cat="IN">before</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">entered</tok> <cons cat="NP"><tok cat="NN">S</tok> <tok cat="NN">phase</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
865284109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">polyclonal</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">invariably</tok></cons> <cons cat="VP"><tok cat="VBD">decreased</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">BCL-6</tok></cons> <tok cat="NN">MRNA</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">germinal</tok> <tok cat="NNS">centers</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP"><tok cat="NN">BCL-6</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">levels</tok></cons> <cons cat="ADJP"><tok cat="JJ">comparable</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBG">resting</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
865284110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Despite</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="JJ">similar</tok> <tok cat="NN">mRNA</tok> <tok cat="NNS">levels</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">BCL-6</tok></cons> <tok cat="NN">protein</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><cons cat="NP"><tok cat="RB">threefold</tok> <tok cat="TO">to</tok> <tok cat="RB">34-fold</tok></cons> <tok cat="JJR">higher</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">germinal</tok> <tok cat="NN">center</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBG">resting</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="NN">BCL-6</tok></cons> <tok cat="NN">protein</tok> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">controlled</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">translational</tok></cons> <cons cat="NP" ref="i8" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">posttranslational</tok></cons> <cons cat="NP" ref="i8" null="RNR"></cons></cons><cons cat="NP" id="i8"><tok cat="NNS">mechanisms</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
865284111@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">observations</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">germinal</tok> <tok cat="NN">center</tok> <tok cat="NN">reaction</tok></cons> <cons cat="VP"><tok cat="VBZ">provides</tok> <cons cat="NP"><tok cat="JJ">unique</tok> <tok cat="NN">activation</tok> <tok cat="NNS">signals</tok> <cons cat="SBAR" id="i9" null="ICH"></cons></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">allow</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">continued</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">high-level</tok></cons></cons> <cons cat="NP"><tok cat="NN">BCL-6</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
865495101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">An</tok> <cons cat="NP"><tok cat="NN">element</tok></cons></cons> <cons cat="ADVP"><tok cat="JJ">upstream</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">delta-globin-encoding</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="VP"><tok cat="VBZ">enhances</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">beta-globin</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">erythroleukemia</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
865495102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">specific</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="JJ">hematopoietic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="JJ">polypryrimidine-binding</tok> <tok cat="NN">factor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">PYBF</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">pyrimidine-rich</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="NP" func="ADV"><cons cat="NP"><tok cat="CD">1</tok> <tok cat="NN">kb</tok> <tok cat="JJ">upstream</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">delta-globin-encoding</tok> <tok cat="NN">gene</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HBD</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@36@@oe@16-12-2010
865495103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">developmental</tok> <tok cat="NN">stage-specificity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PYBF</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">location</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="VBG">binding</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">fetal</tok> <tok cat="CC">and</tok> <tok cat="JJ">adult</tok> <tok cat="NN">beta-globin</tok> <tok cat="LRB">(</tok><tok cat="NN">HBB</tok><tok cat="RRB">)</tok><tok cat="JJ">-like</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">PBYF</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="VBG">binding</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">function</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">fetal-to-adult</tok> <cons cat="NP"><tok cat="NN">globin</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">switching</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
865495104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">describe</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">383-bp</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">delta383</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">99-bp</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">delta99</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">sequences</tok></cons></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">PYBF-binding</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">various</tok> <cons cat="NP"><tok cat="NN">globin</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">non-globin</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">promoters</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">transient</tok> <tok cat="NN">assay</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">cat</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">erythroleukemia</tok> <tok cat="LRB">(</tok><tok cat="NN">MEL</tok><tok cat="RRB">)</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">abundant</tok> <cons cat="NP"><tok cat="NN">PYBF</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@55@@oe@16-12-2010
865495105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">delta383</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">delta99</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="VP"><tok cat="VBP">enhance</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cat</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">plasmids</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">adult</tok> <tok cat="NN">globin</tok> <tok cat="LRB">(</tok><tok cat="NN">HBB</tok><tok cat="RRB">)</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">similar</tok> <tok cat="NNS">constructs</tok></cons></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">fetal</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">A</tok> <tok cat="NN">gamma-</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">globin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HBG1</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">simian</tok> <tok cat="NN">virus</tok> <tok cat="CD">40</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SV40</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NNS">promoters</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">enhanced</tok><cons cat="NP" ref="i8" null="NONE"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@52@@oe@16-12-2010
865495106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">PYBF</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">pyrimidine-rich</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="ADVP"><tok cat="JJ">upstream</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">HBD</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="VP"><tok cat="VB">enhance</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">HBB</tok></cons> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="JJ">erythroid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
865710101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">novel</tok> <cons cat="NP"><tok cat="NN">interferon</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">factor</tok> <tok cat="NN">family</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">ICSAT/Pip/LSIRF</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">negatively</tok></cons> <cons cat="VP"><tok cat="VBZ">regulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">interferon-regulated</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@16-12-2010
865710102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">novel</tok> <tok cat="NN">cDNA</tok> <tok cat="NN">clone</tok></cons></cons> <cons cat="VP"><tok cat="NN">encoding</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interferon</tok> <tok cat="LRB">(</tok><tok cat="NN">IFN</tok><tok cat="RRB">)</tok> <tok cat="NN">consensus</tok> <tok cat="JJ">sequence-binding</tok> <tok cat="NN">protein</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ICSAT</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">homolog</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">recently</tok> <tok cat="VBN">cloned</tok></cons> <cons cat="NP"><tok cat="NN">Pip/LSIRF</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@41@@oe@16-12-2010
865710103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">ICSAT</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">structurally</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="RBS">most</tok> <tok cat="RB">closely</tok> <tok cat="JJ">related</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">previously</tok> <tok cat="VBN">cloned</tok></cons> <cons cat="NP"><tok cat="NN">ICSBP</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">member</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IFN</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">factor</tok> <tok cat="LRB">(</tok><tok cat="NN">IRF</tok><tok cat="RRB">)</tok> <tok cat="NN">family</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interferon</tok> <tok cat="NN">consensus</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">ICSs</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">many</tok> <tok cat="NNS">promoters</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">IFN-regulated</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
865710104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Among</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="VP"><tok cat="VBN">investigated</tok><cons cat="NP" null="NONE"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i3" role="SBJ"><tok cat="NN">ICSAT</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">abundantly</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok> <tok cat="LRB">(</tok><tok cat="NN">HTLV-1</tok><tok cat="RRB">)</tok><tok cat="JJ">-infected</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
865710105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i4"><tok cat="WRB">When</tok></cons> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HTLV-1</tok> <tok cat="NN">tax</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="ADVP" ref="i4" null="T"></cons></cons></cons></cons><tok cat="CC">or</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">phorbol</tok> <tok cat="NN">myristake</tok> <tok cat="NN">acetate-A23187</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">used</tok><cons cat="NP" ref="i6" null="NONE"></cons><cons cat="ADVP" ref="i4" null="T"></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="NN">ICSAT</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">cells</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">otherwise</tok></cons> <cons cat="VP"><tok cat="VBP">do</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">express</tok> <cons cat="NP"><tok cat="NN">ICSAT</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
865710106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i9"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ICSAT</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="CD">four</tok> <tok cat="JJ">different</tok> <cons cat="NP"><tok cat="NNS">ICSs</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">tested</tok><cons cat="NP" ref="i10" null="NONE"></cons><cons cat="ADVP" ref="i9" null="T"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">relative</tok> <tok cat="NNS">differences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">binding</tok> <tok cat="NNS">affinities</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">those</tok> <cons cat="NP"><tok cat="NNS">ICSs</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">determined</tok><cons cat="NP" ref="i11" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
865710107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">study</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">functional</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ICSAT</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">performed</tok> <cons cat="NP"><tok cat="NN">cotransfection</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">embryonal</tok> <tok cat="NN">carcinoma</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">N-Tera2</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
865710108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i13" role="SBJ"><tok cat="NN">ICSAT</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="S"><cons cat="NP" ref="i13" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">possess</tok> <cons cat="NP"><tok cat="JJ">repressive</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">over</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">gene</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">IFN</tok></cons> <tok cat="NN">stimulation</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">IRF-1</tok></cons> <tok cat="NN">cotransfection</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
865710109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Such</tok> <tok cat="JJ">repressive</tok> <tok cat="NN">function</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">similar</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="VP"><tok cat="VBN">seen</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IRF-2</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">ICSBP</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
865710110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">ICSAT</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">different</tok> <tok cat="JJ">repressive</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IRF-2</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">ICSBP</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">some</tok> <cons cat="NP"><tok cat="JJ">IFN-responsive</tok> <tok cat="NN">reporter</tok> <tok cat="NNS">constructs</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
865710111@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="LQT">"</tok><tok cat="JJ">differential</tok> <tok cat="NN">repression</tok><tok cat="RQT">"</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i14" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">multiple</tok> <tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">repressor</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="JJ">repressive</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">IFN-responsive</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
865711501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">alpha</tok> <tok cat="NN">interferon</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="VB">gamma</tok> <tok cat="NN">interferon</tok></cons></cons> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">phorbol</tok> <tok cat="NNS">esters</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphatase</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@16-12-2010
865711502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Previous</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">indicated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NNS">oncoproteins</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">transformation</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">ester</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><tok cat="NN">alpha/beta</tok> <tok cat="NN">interferon</tok> <tok cat="LRB">(</tok><tok cat="NN">IFN-alpha/beta</tok><tok cat="RRB">)</tok><tok cat="JJ">-induced</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@31@@oe@16-12-2010
865711503@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i2"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">promoters</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">growth</tok></cons></cons></cons> <cons cat="VP"><tok cat="VB">exert</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP" ref="i2" null="T"></cons></cons></cons></cons></cons><cons cat="VP"><tok cat="VBP">vary</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJS">most</tok> <tok cat="NNS">instances</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBP">involve</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">disruption</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">IFN-alpha/beta-induced</tok> <tok cat="NN">transcription</tok> <tok cat="NN">complex</tok> <tok cat="NN">ISGF3</tok></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="JJ">such</tok> <tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">component</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">complex</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">48-kDa</tok> <tok cat="NN">ISGF3gamma</tok> <tok cat="NN">protein</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">interferon-stimulated</tok> <tok cat="NN">response</tok> <tok cat="NN">element</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ISRE</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@55@@oe@16-12-2010
865711504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">report</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="UCP" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">ester</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">dramatically</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">IFN-alpha/B-stimulated</tok> <tok cat="JJ">early</tok> <tok cat="NN">response</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="FRAG"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">involve</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">abrogation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ISRE</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ISGF3gamma</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
865711505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Phorbol</tok> <tok cat="NN">ester</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IFN</tok> <tok cat="JJ">alpha-stimulated</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Stat1alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Stat2</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Stat3</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok></cons> <cons cat="NP"><tok cat="NN">Tyk2</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Stat1alpha</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
865711506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">IFNalpha-stimulated</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Jak1</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">alpha</tok> <tok cat="NN">subunit</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IFN-alpha</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unaffected</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">12-myristate</tok> <tok cat="NN">13-acetate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PMA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
865711507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">PMA</tok></cons> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">dephosphorylation</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Tyk2</tok></cons></cons> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Jak1</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" id="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">IFN</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
865711508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Pretreatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">vanadate</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">prevented</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PMA</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">regard</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">PMA-induced</tok> <cons cat="NP"><tok cat="NN">Tyk2</tok></cons> <tok cat="NN">dephosphorylation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
865711509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">observations</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PMA</tok></cons> <cons cat="VP"><tok cat="VBZ">exerts</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphatase</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">selectively</tok></cons> <cons cat="VP"><tok cat="VBZ">regulates</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">Tyk2</tok></cons> <cons cat="NP" ref="i8" null="RNR"></cons></cons><cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">Jak1</tok></cons> <cons cat="NP" null="RNR"></cons></cons><cons cat="NP" id="i8"><tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
865714301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">myeloid-cell-specific</tok> <tok cat="NN">c-fes</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">Sp1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">PU.1</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@16-12-2010
865714302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">protein</tok> <tok cat="NN">product</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">c-fps/fes</tok> <tok cat="LRB">(</tok><tok cat="NN">c-fes</tok><tok cat="RRB">)</tok> <tok cat="NN">proto-oncogene</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">normal</tok> <tok cat="NN">development</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">macrophages</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">neutrophils</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@26@@oe@16-12-2010
865714303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="NN">mRNA</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">c-fes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><cons cat="ADVP"><tok cat="RB">exclusively</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">vascular</tok> <tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="NNS">mammals</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
865714304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">13-kilobase-pair</tok> <tok cat="LRB">(</tok><tok cat="NN">kb</tok><tok cat="RRB">)</tok> <tok cat="JJ">human</tok> <tok cat="NN">c-fes</tok> <tok cat="NN">transgene</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">exhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">expression</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">mice</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">confer</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">myeloid-cell-specific</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">c-fes</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">defined</tok><cons cat="NP" ref="i4" null="NONE"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
865714305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Transient-transfection</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">plasmids</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">446</tok> <tok cat="NN">bp</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-fes</tok> <tok cat="JJ">5'-flanking</tok> <tok cat="NNS">sequences</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">linked</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">luciferase</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">active</tok></cons> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">exclusively</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
865714306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">No</tok> <tok cat="JJ">other</tok> <tok cat="NN">DNA</tok> <tok cat="NN">element</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">13-kb</tok> <tok cat="JJ">human</tok> <tok cat="NN">c-fes</tok> <tok cat="NN">locus</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP"><tok cat="JJ">positive</tok> <tok cat="JJ">cis-acting</tok> <tok cat="NNS">elements</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">exception</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">weakly</tok> <tok cat="JJ">active</tok></cons> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">3'-flanking</tok> <tok cat="NNS">sequences</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
865714307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">DNase</tok> <tok cat="CD">I</tok> <tok cat="NN">footprinting</tok> <tok cat="NNS">assays</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">four</tok> <tok cat="JJ">distinct</tok> <tok cat="NNS">sites</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">bind</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-408</tok> <tok cat="TO">to</tok> <tok cat="CD">-386</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">-293</tok> <tok cat="TO">to</tok> <tok cat="CD">-254</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">-76</tok> <tok cat="TO">to</tok> <tok cat="CD">-65</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-34</tok> <tok cat="TO">to</tok> <tok cat="CD">+3</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
865714308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="CD">two</tok> <tok cat="NNS">footprints</tok></cons> <cons cat="VP"><tok cat="VBD">resided</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">sequences</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">largely</tok> <tok cat="JJ">dispensable</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
865714309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Plasmids</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">151</tok> <tok cat="NN">bp</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">5'-flanking</tok> <tok cat="NNS">sequences</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">confer</tok> <cons cat="NP"><tok cat="JJ">myeloid-cell-specific</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
865714310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">analyses</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">151-bp</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">protein</tok> <tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Sp1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">PU.1</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and/or</tok> <cons cat="NP"><tok cat="NN">Elf-1</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">novel</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
865714311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">This</tok> <tok cat="JJ">unidentified</tok> <tok cat="NN">factor</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="NP"><cons cat="ADVP"><tok cat="RB">immediately</tok> <tok cat="JJ">3'</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">PU.1/Elf-1</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">myeloid</tok> <tok cat="NN">cell</tok> <tok cat="JJ">specific</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
865714312@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Mutation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">PU.1/Elf-1</tok></cons> <tok cat="NN">site</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">3'</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">FP4-3'</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">context</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">c-fes</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">substantially</tok> <tok cat="VBN">reduced</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NNS">transfections</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
865714313@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="JJ">transient-cotransfection</tok> <tok cat="NN">assay</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">PU.1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><tok cat="CC">and</tok> <tok cat="RB">not</tok> <cons cat="NP"><tok cat="NN">Elf-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">transactivate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">c-fes</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">nonmyeloid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
865714314@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">c-fes</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">strong</tok> <cons cat="NP"><tok cat="JJ">myeloid-cell-specific</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" id="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">Sp1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">PU.1</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
865715301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Transcriptional</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">RNA</tok> <tok cat="NN">polymerase</tok> <tok cat="JJ">III-dependent</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok> <tok cat="NN">tax</tok> <tok cat="NN">protein</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@16-12-2010
865715302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="JJ">virus-encoded</tok> <tok cat="NN">tax</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potent</tok> <tok cat="NN">activator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="JJ">many</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><cons cat="NP" id="i1"><tok cat="NNS">genes</tok></cons></cons> <cons cat="VP"><tok cat="VBN">transcribed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">RNA</tok> <tok cat="NN">polymerase</tok> <tok cat="CD">II</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@16-12-2010
865715303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">find</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">chromatin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NNS">extracts</tok></cons></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="JJ">1-infected</tok> <tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">support</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJR">higher</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">5S</tok> <tok cat="NN">rRNA</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">tRNA</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><cons cat="NP" id="i2"><tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">than</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">chromatin</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NNS">extracts</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">uninfected</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
865715304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">protein</tok> <tok cat="NN">Tax</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">likely</tok> <tok cat="JJ">responsible</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJR">higher</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="VBN">purified</tok> <tok cat="NN">Tax</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">stimulate</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">genes</tok></cons> <cons cat="UCP" syn="COOD"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i4"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">added</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">uninfected</tok> <tok cat="NN">cell</tok> <tok cat="NN">extract</tok></cons></cons> <cons cat="ADVP" ref="i4" null="T"></cons></cons></cons></cons><tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">reconstituted</tok> <tok cat="NNS">systems</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
865715305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">Both</tok> <cons cat="NP"><tok cat="JJ">limiting-component</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">assays</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NNS">assays</tok></cons></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">class</tok> <tok cat="CD">III</tok> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="NP"><tok cat="NN">TFIIIB</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">principle</tok> <tok cat="NN">target</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Tax</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
865715306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Surprisingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">find</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Tax</tok></cons> <cons cat="VP"><tok cat="VBZ">increases</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">effective</tok> <tok cat="NN">concentration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">active</tok> <tok cat="NN">TFIIIB</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
865715307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">Tax</tok></cons> <cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">RNA</tok> <tok cat="NN">polymerase</tok> <tok cat="JJ">III-dependent</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">accelerating</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">rate</tok></cons> <tok cat="CC">and/or</tok> <cons cat="NP"><tok cat="NN">extent</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">initiation</tok> <tok cat="NN">complex</tok></cons> <tok cat="NN">assembly</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
865919001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">Transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">T</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">B</tok></cons></cons> <tok cat="NNS">lymphocytes</tok></cons></cons></cons><tok cat="COLON">--</tok><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">basic</tok> <tok cat="NN">research</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">clinical</tok> <tok cat="NNS">perspectives</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">gastroenterology</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
865919002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">Tissue</tok> <tok cat="JJ">specific</tok></cons> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">fascinating</tok> <tok cat="JJ">new</tok> <tok cat="NN">field</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">molecular</tok> <tok cat="NN">immunology</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
865919003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">review</tok></cons> <cons cat="VP"><tok cat="VBZ">summarizes</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">data</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">promoters</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">enhancers</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="JJ">trans-acting</tok> <tok cat="NNS">factors</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
865919004@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i74" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">structural</tok> <tok cat="NNS">classes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">described</tok> <cons cat="NP" ref="i74" null="NONE"></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i75" role="SBJ"><cons cat="NP"><tok cat="JJ">basic</tok> <tok cat="NNS">methods</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">detection</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">analysis</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">detailed</tok><cons cat="NP" ref="i75" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
865919005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i77" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RBS">most</tok> <tok cat="JJ">important</tok></cons> <tok cat="JJ">trans-acting</tok> <tok cat="NNS">factors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">T</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">B</tok></cons></cons> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="ADVP"><tok cat="FW">e.g.</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kB</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT</tok></cons></cons> <tok cat="NNS">families</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">functional</tok> <tok cat="NN">importance</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">described</tok><cons cat="NP" ref="i77" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
865919006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i78" role="SBJ"><cons cat="NP"><tok cat="JJ">Several</tok> <tok cat="NNS">methods</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">down-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="NP"><cons cat="NP" ref="i78" null="NONE"></cons><cons cat="SBAR"><cons cat="WHNP" id="i79"><tok cat="IN">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i79" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">relevant</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">disease</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
865919007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i80" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">discussed</tok> <cons cat="NP" ref="i80" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <tok cat="NN">regard</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">potential</tok> <tok cat="JJ">clinical</tok> <tok cat="NN">relevance</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">gastroenterology</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
866266601@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">DNA</tok> <tok cat="NN">triplex</tok> <tok cat="NN">formation</tok></cons> <cons cat="ADVP"><tok cat="RB">selectively</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">granulocyte-macrophage</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
866266602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Granulocyte-macrophage</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GM-CSF</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">hemopoietic</tok> <tok cat="NN">growth</tok> <tok cat="NN">factor</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" id="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="ADJP"><tok cat="VBN">activated</tok></cons> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNS">fibroblasts</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@16-12-2010
866266603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="NN">GM-CSF</tok></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">appear</tok> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">hemopoiesis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="NN">overexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GM-CSF</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathogenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">some</tok> <tok cat="NNS">diseases</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NN">leukemia</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">chronic</tok> <tok cat="NN">inflammation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
866266604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">An</tok> <tok cat="NN">NF-kappaB/Rel</tok> <tok cat="NN">binding</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GM-CSF</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">termed</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="DT">the</tok> <tok cat="NN">kappaB</tok> <tok cat="NN">element</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">controlling</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons> <tok cat="NNS">assays</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">stimuli</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
866266605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">oligonucleotide-directed</tok></cons> <cons cat="ADJP"><tok cat="JJ">triple</tok></cons></cons> <tok cat="NN">helix</tok> <tok cat="NN">formation</tok></cons> <cons cat="PP"><tok cat="IN">across</tok> <cons cat="NP"><tok cat="DT">this</tok> <cons cat="NP"><tok cat="JJ">regulatory</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potential</tok> <tok cat="NN">tool</tok></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i19" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GM-CSF</tok></cons> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons><cons cat="ADVP" ref="i19" null="T"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
866266606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">15-base</tok> <tok cat="NN">oligonucleotide</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">GM3</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">targeted</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">purine-rich</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GM-CSF</tok> <tok cat="JJ">proximal</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">overlaps</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">kappaB</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
866266607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Gel</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">DNase</tok> <tok cat="CD">I</tok></cons> <tok cat="NN">footprinting</tok></cons></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">GM3</tok></cons> <cons cat="VP"><tok cat="VBD">formed</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">sequence-specific</tok> <tok cat="NN">collinear</tok> <tok cat="NN">triplex</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <cons cat="NP"><tok cat="JJ">double-stranded</tok> <tok cat="NN">DNA</tok> <tok cat="NN">target</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
866266608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Triplex</tok> <tok cat="NN">formation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">GM3</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">blocked</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">recombinant</tok> <cons cat="NP" ref="i8" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <cons cat="NP" ref="i8" null="RNR"></cons></cons><cons cat="NP" id="i8"><tok cat="NN">NF-kappaB</tok> <tok cat="NNS">proteins</tok> <tok cat="VBG">binding</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GM-CSF</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
866266609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">GM3</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">selective</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="JJ">lymphotrophic</tok> <tok cat="NN">virus-1</tok> <tok cat="NN">Tax</tok></cons> <tok cat="JJ">transactivator-induced</tok> <cons cat="NP"><tok cat="NN">luciferase</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">reporter</tok> <tok cat="NN">construct</tok></cons> <cons cat="VP"><tok cat="VBN">driven</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GM-CSF</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
866266610@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">GM3</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><cons cat="ADVP"><tok cat="RB">greatly</tok></cons> <tok cat="VBD">reduced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">concentration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">endogenous</tok> <cons cat="NP"><tok cat="NN">GM-CSF</tok> <tok cat="NN">mRNA</tok></cons></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">stimuli</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="CD">3</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
866266611@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">kappaB</tok> <tok cat="NN">element</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GM-CSF</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">central</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">endogenous</tok> <cons cat="NP"><tok cat="NN">GM-CSF</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
866266612@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Colinear</tok> <tok cat="NN">triplex</tok> <tok cat="NN">formation</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">acts</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">selective</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">repressor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GM-CSF</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">potential</tok> <tok cat="JJ">therapeutic</tok></cons> <tok cat="NN">application</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cases</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">undesirable</tok> <tok cat="NN">overexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
866284501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Cooperation</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">core</tok> <tok cat="NN">binding</tok> <tok cat="NN">factor</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">adjacent</tok> <tok cat="NN">promoter</tok> <tok cat="NNS">elements</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">contributes</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">tissue-specific</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">interleukin-3</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
866284502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="JJ">Tissue-specific</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-3</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-3</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cis-acting</tok> <tok cat="NNS">elements</tok></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">315</tok> <tok cat="NN">base</tok> <tok cat="NNS">pairs</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">start</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
866284503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">achieved</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">part</tok></cons></cons> <tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">positive</tok> <tok cat="NNS">activities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Elf-1</tok></cons></cons> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-3</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
866284504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">contribution</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">T</tok> <tok cat="JJ">cell-specific</tok></cons> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NN">promoter</tok> <tok cat="NNS">sites</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">assessed</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">transient</tok> <tok cat="NN">expression</tok> <tok cat="NN">assay</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-3</tok> <tok cat="NN">promoter</tok> <tok cat="NNS">constructs</tok></cons> <cons cat="VP"><tok cat="VBN">linked</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">luciferase</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">focusing</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">initially</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">core</tok> <tok cat="NN">binding</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CBF</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">site</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" id="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="JJ">footprinted</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">upon</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@49@@oe@16-12-2010
866284505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">Activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CBF</tok> <tok cat="NN">site</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">critically</tok> <tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">adjacent</tok> <tok cat="NN">activator</tok> <tok cat="NN">site</tok> <tok cat="CD">Act-1</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
866284506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Together</tok></cons> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Act-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CBF</tok></cons></cons> <tok cat="NNS">sites</tok></cons> <cons cat="VP"><tok cat="VBP">form</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">functional</tok> <tok cat="NN">unit</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AC</tok> <tok cat="NN">unit</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">dual</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
866284507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">AC</tok> <tok cat="NN">unit</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="VB">enhance</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">basal</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">promoters</tok></cons></cons> <cons cat="PP"><cons cat="ADVP"><tok cat="CC">both</tok></cons> <tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">fibroblasts</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
866284508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">further</tok> <tok cat="JJ">inducible</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">fibroblasts</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
866284509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <tok cat="NN">addition</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">already</tok> <tok cat="VBN">identified</tok></cons> <tok cat="NN">NIP</tok> <tok cat="NN">repressor</tok> <tok cat="NN">site</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><tok cat="NN">evidence</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">presented</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">second</tok> <tok cat="NN">repressor</tok> <tok cat="NN">region</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">restricts</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">fibroblasts</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
866284510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="JJ">positive</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">element</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">mapped</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-3</tok> <tok cat="NN">promoter</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="NN">nucleotide</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-180</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-210</tok></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i11"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
866284511@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Together</tok></cons> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-3</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">specified</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">single</tok> <tok cat="JJ">tissue-specific</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <tok cat="RB">instead</tok> <cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">multiple</tok> <tok cat="VBG">interacting</tok> <tok cat="NNS">elements</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i13"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i13" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="JJ">positive</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="JJ">negative</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">fibroblasts</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
866292801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NNS">Receptors</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="LRB">(</tok><tok cat="NN">IL</tok><tok cat="RRB">)</tok><tok cat="CD">-10</tok> <cons cat="NP" null="QSTN"></cons><cons cat="NP" ref="i2" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">IL-6-type</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><cons cat="NP" id="i2"><tok cat="NNS">cytokines</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">use</tok> <cons cat="NP"><tok cat="JJ">similar</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="NN">inducing</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="NN">IL-6</tok> <tok cat="NN">response</tok> <tok cat="NNS">elements</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@22@@oe@16-12-2010
866292802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">cytoplasmic</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="CD">10</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-10R</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <cons cat="NP"><tok cat="NN">box</tok> <tok cat="CD">3</tok> <tok cat="NN">sequence</tok> <tok cat="NNS">motifs</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" id="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">signal-transducing</tok> <tok cat="NN">receptor</tok> <tok cat="NNS">subunits</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">IL-6-type</tok> <tok cat="NNS">cytokines</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">noted</tok> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBG">activating</tok> <cons cat="NP"><tok cat="NN">STAT3</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBG">inducing</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="JJ">IL-6-responsive</tok> <tok cat="NNS">elements</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@45@@oe@16-12-2010
866292803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-10R</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">signaling</tok> <tok cat="NNS">functions</tok></cons> <cons cat="ADJP"><tok cat="JJ">similar</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IL-6R</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">normally</tok></cons> <tok cat="VBG">expressing</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NNS">leukocytes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">B-</tok><cons cat="NP" null="QSTN"></cons><cons cat="NP" ref="i6" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">T-</tok><cons cat="NP" null="QSTN"></cons><cons cat="NP" ref="i6" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NK-cell</tok> <cons cat="NP" ref="i6" null="RNR"></cons></cons><cons cat="NP" id="i6"><tok cat="NNS">lineages</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">either</tok> <tok cat="NN">cytokine</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
866292804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">Both</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons> <cons cat="VP"><tok cat="VBD">activated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">factors</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">bound</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">sis-inducible</tok> <tok cat="NN">element</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">included</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT3</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
866292805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">cell</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">characteristically</tok></cons> <cons cat="VP"><tok cat="VBN">differed</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">that</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2/IL-15</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">interferon</tok> <tok cat="NN">gamma</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
866292806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">capabilities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-10R</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBG">activating</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NNS">proteins</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBG">inducing</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">defined</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">reconstitution</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">receptor</tok> <tok cat="NNS">functions</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">transfected</tok> <tok cat="NN">tissue</tok> <tok cat="NN">culture</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
866292807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">COS-1</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">co-expressing</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <cons cat="NP"><tok cat="NN">IL-10R</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">individual</tok> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">confirmed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">preference</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-10R</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT1</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
866292808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Unlike</tok> <cons cat="NP"><tok cat="JJ">many</tok> <cons cat="NP"><tok cat="NN">hematopoietin</tok> <tok cat="NNS">receptors</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-10R</tok></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="ADVP"><tok cat="RB">detectably</tok></cons> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="NN">STAT5</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
866292809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">IL-10R</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">together</tok></cons> <tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">reporter</tok> <tok cat="NN">gene</tok> <tok cat="NNS">constructs</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="JJ">different</tok> <cons cat="NP"><tok cat="JJ">IL-6-responsive</tok> <tok cat="NN">gene</tok> <tok cat="NNS">elements</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">reconstituted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">hepatoma</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">IL-10</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">comparable</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">IL-6</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
866292810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">regulation</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADVP"><tok cat="RB">appreciably</tok></cons> <cons cat="VP"><tok cat="VBN">modified</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="VBN">enhanced</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
866292811@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">similar</tok> <tok cat="NNS">actions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-10R</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-6R</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">endogenous</tok> <cons cat="NP"><tok cat="JJ">IL-6-responsive</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">hepatoma</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">stably</tok></cons> <tok cat="VBG">expressing</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-10R</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
866292812@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NN">receptor</tok> <tok cat="NNS">functions</tok></cons> <cons cat="VP"><tok cat="VBD">required</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">box</tok> <tok cat="CD">3</tok> <tok cat="NNS">motifs</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mouse</tok> <cons cat="NP"><tok cat="NN">IL-10R</tok></cons> <tok cat="NNS">constructs</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="RB">progressively</tok> <tok cat="VBN">truncated</tok> <tok cat="JJ">cytoplasmic</tok> <tok cat="NNS">domains</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
866292813@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-10R</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="PP"><tok cat="IN">unlike</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">interferon</tok> <tok cat="NN">receptor</tok> <tok cat="NN">family</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">highly</tok> <tok cat="JJ">effective</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">recruiting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">IL-6-type</tok> <tok cat="NN">cytokine</tok> <tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
866296001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Globin</tok> <tok cat="NN">gene</tok> <tok cat="NN">switching</tok><tok cat="PERIOD">.</tok></cons></cons>@@@@1@4@@oe@16-12-2010
866296002@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">In</tok> <tok cat="FW">vivo</tok></cons> <tok cat="JJ">protein-DNA</tok> <tok cat="NNS">interactions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">beta-globin</tok> <tok cat="NN">locus</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">fetal</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">adult</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><cons cat="NP" id="i1"><cons cat="NP"><tok cat="NN">globin</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">program</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
866296003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">characterize</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">protein-DNA</tok> <tok cat="NNS">interactions</tok></cons> <cons cat="ADJP"><tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">developmental</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">beta-globin</tok> <tok cat="NN">locus</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">analyzed</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <tok cat="NN">dimethyl</tok> <tok cat="JJ">sulfate</tok> <tok cat="NN">footprinting</tok></cons></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">fetal</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">adult</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons><cons cat="NP" id="i3"><tok cat="NN">globin</tok> <tok cat="JJ">developmental</tok> <tok cat="NN">program</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@37@@oe@16-12-2010
866296004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">locus</tok> <tok cat="NN">control</tok> <tok cat="NN">region</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LCR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">beta-globin</tok> <tok cat="NN">locus</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <tok cat="NNS">footprints</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">NF-E2</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><cons cat="PRN"><tok cat="LRB">(</tok><tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AP-1</tok></cons><cons cat="NP" null="QSTN"></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok> <tok cat="NNS">motifs</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">remained</tok> <cons cat="NP" role="PRD"><tok cat="DT">the</tok> <tok cat="JJ">same</tok></cons> <cons cat="ADVP"><tok cat="RB">regardless</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i4" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">fetal</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="NN">globin</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">expressed</tok><cons cat="NP" ref="i4" null="NONE"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
866296005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <tok cat="NNS">footprints</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">GT</tok> <tok cat="LRB">(</tok><tok cat="NN">CACCC</tok><tok cat="RRB">)</tok> <tok cat="NNS">motifs</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">differed</tok> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">fetal</tok> <cons cat="NP" ref="i5" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">adult</tok> <cons cat="NP" ref="i5" null="RNR"></cons></cons><cons cat="NP" id="i5"><tok cat="NN">globin</tok> <tok cat="NN">program</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
866296006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NNS">regions</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="RB">actively</tok> <tok cat="VBN">transcribed</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">extensive</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">consistent</tok></cons></cons> <tok cat="NNS">footprints</tok></cons> <cons cat="PP"><tok cat="IN">over</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">canonical</tok> <tok cat="NNS">elements</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CACCC</tok> <cons cat="NP" ref="i6" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CCAAT</tok> <cons cat="NP" ref="i6" null="RNR"></cons></cons><cons cat="NP" id="i6"><tok cat="NNS">motifs</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
866296007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="NN">globin</tok> <tok cat="NN">expressing</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBD">displayed</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RBR">more</tok> <tok cat="JJ">extensive</tok></cons> <tok cat="NNS">footprints</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">than</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">fetal</tok> <tok cat="NN">globin</tok> <tok cat="NN">expressing</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP" null="QSTN"></cons></cons></cons></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">3'</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">sequences</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Agamma-</tok> <cons cat="NP" null="QSTN"></cons><cons cat="NP" ref="i7" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">beta-globin</tok> <cons cat="NP" ref="i7" null="RNR"></cons></cons><cons cat="NP" id="i7"><tok cat="NNS">genes</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="JJ">3'</tok> <tok cat="NNS">elements</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">beta-globin</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
866296008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">bulk</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">protein-DNA</tok> <tok cat="NNS">interactions</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">underlies</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">developmental</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">globin</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">takes</tok> <cons cat="NP"><tok cat="NN">place</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">gamma-</tok> <cons cat="NP" null="QSTN"></cons><cons cat="NP" ref="i9" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">beta-globin</tok> <cons cat="NP" ref="i9" null="RNR"></cons></cons><cons cat="NP" id="i9"><tok cat="NN">gene</tok> <tok cat="NNS">promoters</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="NN">GT</tok> <tok cat="NNS">motifs</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">beta-globin</tok> <tok cat="NN">locus</tok></cons> <cons cat="NP"><tok cat="NN">LCR</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">developmental</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP"><tok cat="NN">beta-globin</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">perhaps</tok></cons> <tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">increasing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">probability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">LCR</tok> <tok cat="NN">holocomplex</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">fetal</tok> <cons cat="NP" ref="i11" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">adult</tok> <cons cat="NP" ref="i11" null="RNR"></cons></cons><cons cat="NP" id="i11"><tok cat="NN">globin</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@70@@oe@16-12-2010
866302201@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Octamer</tok> <tok cat="NN">binding</tok> <tok cat="NNS">factors</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <cons cat="NP"><tok cat="NN">coactivator</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">PU.1</tok> <tok cat="LRB">(</tok><tok cat="NN">spi-1</tok><tok cat="RRB">)</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@16-12-2010
866302202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">PU.1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">spi-1</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">member</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Ets</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">family</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">predominantly</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">myeloid</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">B</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><cons cat="NP" id="i2"><tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">many</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons><cons cat="NP" id="i3"><tok cat="NNS">genes</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">critical</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">development</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">both</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">lineages</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@42@@oe@16-12-2010
866302203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRPP">Our</tok> <tok cat="JJ">previous</tok> <tok cat="NNS">studies</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNP">Chen</tok><tok cat="COMMA">,</tok> <tok cat="NNP">H.M.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Ray-Gallet</tok><tok cat="COMMA">,</tok> <tok cat="NNP">D.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Zhang</tok><tok cat="COMMA">,</tok> <tok cat="NNP">P.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Hetherington</tok><tok cat="COMMA">,</tok> <tok cat="NNP">C.J.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Gonzalez</tok><tok cat="COMMA">,</tok> <tok cat="NNP">D.A.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Zhang</tok><tok cat="COMMA">,</tok> <tok cat="NNP">D.-E.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Moreau-Gachelin</tok><tok cat="COMMA">,</tok> <tok cat="NNP">F.</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNP">Tenen</tok><tok cat="COMMA">,</tok> <tok cat="NNP">D.G.</tok></cons></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">1995</tok></cons><tok cat="RRB">)</tok></cons> <cons cat="NP"><tok cat="NN">Oncogene</tok> <tok cat="CD">11</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">1549-1560</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">PU.1</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">directs</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="JJ">type-specific</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PU.1</tok></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">own</tok> <tok cat="NN">promoter</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">autoregulatory</tok> <tok cat="NN">loop</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@72@@oe@16-12-2010
866302204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">PU.1</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><cons cat="ADVP" syn="COOD"><cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">highly</tok></cons></cons> <tok cat="JJ">functional</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="ADVP"><tok cat="RB">as</tok> <tok cat="RB">well</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
866302205@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Oct-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Oct-2</tok></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="NN">base</tok> <tok cat="NN">pair</tok> <tok cat="CD">-55</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">site</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="VP"><tok cat="VBN">protected</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
866302206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="CD">two</tok> <tok cat="JJ">other</tok> <tok cat="NNS">sites</tok> <cons cat="NP" id="i5" null="ICH"></cons></cons><cons cat="VP"><tok cat="VBP">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">Sp1</tok> <tok cat="NN">binding</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">adjacent</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">octamer</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">PU.1</tok> <tok cat="NN">autoregulatory</tok> <tok cat="NN">site</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
866302207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <cons cat="NP"><tok cat="NN">coactivator</tok></cons></cons> <cons cat="NP"><tok cat="NN">OBF-1/Bob1/OCA-B</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">only</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">OBF-1/Bob1/OCA-B</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">transactivate</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">PU.1</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">HeLa</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
866302208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="JJ">restricted</tok> <tok cat="NN">coactivator</tok></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">responsible</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="JJ">specific</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PU.1</tok></cons> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">octamer</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
866306001@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="JJ">Tissue-specific</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">gammac</tok> <tok cat="NN">chain</tok> <tok cat="NN">gene</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">depends</tok> <cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">Ets</tok> <tok cat="NN">binding</tok> <tok cat="NN">site</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">GA-binding</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@16-12-2010
866306002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">gammac</tok> <tok cat="NN">chain</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">subunit</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">multiple</tok> <cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NNS">receptors</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="LRB">(</tok><tok cat="NN">IL</tok><tok cat="RRB">)</tok><tok cat="CD">-2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-7</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-9</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-15</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i2"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="WHPP"><tok cat="IN">of</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">restricted</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NNS">lineages</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@37@@oe@16-12-2010
866306003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">defect</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">gammac</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">X-linked</tok></cons> <cons cat="ADJP"><tok cat="JJ">severe</tok></cons> <cons cat="ADJP"><tok cat="JJ">combined</tok></cons></cons> <tok cat="NN">immunodeficiency</tok></cons> <cons cat="VP"><tok cat="VBN">characterized</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">block</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
866306004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">In</tok> <tok cat="NN">order</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="ADVP"><tok cat="RB">better</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">characterize</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">gammac</tok> <tok cat="NN">promoter</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">define</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">minimal</tok> <tok cat="JJ">tissue-specific</tok> <tok cat="NN">promoter</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="JJ">progressive</tok> <cons cat="NP"><tok cat="JJ">5'-deletion</tok> <tok cat="NNS">constructs</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">segment</tok></cons> <cons cat="VP"><tok cat="VBG">extending</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">1053</tok> <tok cat="NN">base</tok> <tok cat="NNS">pairs</tok> <tok cat="RB">upstream</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">major</tok> <tok cat="NN">transcription</tok> <tok cat="NN">start</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">generated</tok> <cons cat="NP" ref="i3" null="NONE"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">tested</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">various</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">hematopoietic</tok></cons> <cons cat="NP" ref="i4" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">nonhematopoietic</tok></cons> <cons cat="NP" ref="i4" null="RNR"></cons></cons><cons cat="NP" id="i4"><tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@49@@oe@16-12-2010
866306005@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="CD">-1053/+34</tok> <tok cat="NN">construct</tok></cons></cons> <cons cat="VP"><tok cat="VBD">allowed</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NN">origin</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">tissue</tok> <tok cat="NN">specificity</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">conserved</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="JJ">other</tok> <tok cat="NNS">constructs</tok></cons> <cons cat="VP"><tok cat="VBN">tested</tok><cons cat="NP" null="NONE"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
866306006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">region</tok></cons> <cons cat="ADVP"><tok cat="JJ">downstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">-90</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">appeared</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">critical</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">basal</tok> <tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
866306007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">potential</tok> <cons cat="NP"><tok cat="NN">Ets</tok> <tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons></cons> <cons cat="VP"><tok cat="VBN">conserved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">gammac</tok> <tok cat="NN">promoter</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i6"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="WHPP"><tok cat="IN">of</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons></cons> <cons cat="S"><cons cat="NP" ref="i6" id="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">determined</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">mutational</tok> <tok cat="NN">analysis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
866306008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">functional</tok> <cons cat="NP"><tok cat="NN">Ets</tok> <tok cat="NN">binding</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Ets</tok> <tok cat="NN">family</tok> <tok cat="NNS">proteins</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">principally</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">GA-binding</tok> <tok cat="NN">protein</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Elf-1</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">transactivated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">GABPalpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><cons cat="NP"><tok cat="NN">-beta</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">synergistically</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
866306009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="ADVP" adv="TMP"><tok cat="RB">already</tok></cons> <cons cat="VP"><tok cat="VBN">reported</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL2Rbeta</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">GA-binding</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">essential</tok> <tok cat="NN">component</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">gammac</tok> <tok cat="JJ">basal</tok> <tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
866306010@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">GABP</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">restricted</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NN">lineage</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">other</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">tissue-specific</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">gammac</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
866314801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">3'</tok></cons> <tok cat="TO">--&gt;</tok> <cons cat="NP"><tok cat="JJ">5'</tok></cons></cons> <tok cat="NN">XPB</tok> <tok cat="NN">helicase</tok> <tok cat="NN">defect</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">repair/transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">TFIIH</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">xeroderma</tok> <tok cat="NN">pigmentosum</tok> <tok cat="NN">group</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">affects</tok> <cons cat="NP"><tok cat="CC">both</tok> <tok cat="NN">DNA</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">repair</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
866314802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">XPB</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">subunit</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">basal</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">TFIIH</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i53"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i53" id="i54" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i54" null="NONE"></cons><cons cat="PP" id="i55"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">nucleotide</tok> <tok cat="NN">excision</tok> <tok cat="NN">repair</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NER</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">potentially</tok></cons> <cons map="i55" cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">cycle</tok> <tok cat="NN">regulation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
866314803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">frameshift</tok> <tok cat="NN">mutation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">3'-end</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">XPB</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">responsible</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">concurrence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">disorders</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">xeroderma</tok> <tok cat="NN">pigmentosum</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">XP</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Cockayne</tok><tok cat="POS">'s</tok></cons> <tok cat="NN">syndrome</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CS</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
866314804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i56" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP"><tok cat="NN">TFIIH</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP" id="i58"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">patient</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">XP11BE</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i60"><tok cat="WP">who</tok></cons> <cons cat="S"><cons cat="NP" ref="i60" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">carries</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">frameshift</tok> <tok cat="NN">mutation</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="PRN" id="i59"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TFIIHmut</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons map="i58" cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mother</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">patient</tok></cons></cons></cons></cons> <cons map="i59" cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TFIIHwt</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="S"><cons cat="NP" ref="i56" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">biochemical</tok> <tok cat="NNS">consequences</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mutation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
866314805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" ref="i61" role="SBJ" null="NONE"></cons><cons cat="ADJP" role="PRD"><tok cat="JJ">identical</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">composition</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">stoichiometry</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">TFIIHwt</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i61" role="SBJ"><tok cat="NN">TFIIHmut</tok></cons> <cons cat="VP"><tok cat="VBZ">shows</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="VBN">reduced</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">3'</tok></cons> <tok cat="TO">--&gt;</tok> <cons cat="NP"><tok cat="JJ">5'</tok></cons></cons> <tok cat="NN">XPB</tok> <tok cat="NN">helicase</tok> <tok cat="NN">activity</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
866314806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i64" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">helicase</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">DNA-dependent</tok> <tok cat="NN">ATPase</tok></cons></cons> <tok cat="NNS">activities</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i64" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">mutated</tok> <tok cat="JJ">recombinant</tok> <tok cat="NN">XPB</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
866314807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">XPB</tok> <tok cat="NN">mutation</tok></cons> <cons cat="VP"><tok cat="VBZ">causes</tok> <cons cat="VP"><tok cat="DT">a</tok> <tok cat="JJ">severe</tok> <tok cat="NN">NER</tok> <tok cat="NN">defect</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
866314808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">basal</tok> <tok cat="NN">transcription</tok> <tok cat="NN">activity</tok></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
866314809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">latter</tok> <tok cat="NN">defect</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">explanation</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">many</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">XP</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CS</tok></cons></cons> <tok cat="NNS">symptoms</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i65"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i65" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">difficult</tok> <cons cat="SBAR"><cons cat="WHNP" id="i66" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">rationalize</tok> <cons cat="NP" ref="i66" null="T"></cons><cons cat="PP"><tok cat="VBN">based</tok> <cons cat="PP"><tok cat="RB">solely</tok> <tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">NER</tok> <tok cat="NN">defect</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
866314810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">work</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">presents</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="JJ">detailed</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">naturally</tok> <tok cat="VBG">occurring</tok></cons> <tok cat="NN">mutation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">basal</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">supports</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">concept</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">combined</tok> <tok cat="NN">XP/CS</tok> <tok cat="JJ">clinical</tok> <tok cat="NN">entity</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">actually</tok></cons> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">result</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">combined</tok> <tok cat="NN">transcription/repair</tok> <tok cat="NN">deficiency</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
866317401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="JJ">Multiple</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">interleukin</tok> <tok cat="CD">9</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@16-12-2010
866317402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">genetic</tok> <tok cat="NNS">elements</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">regulatory</tok> <tok cat="NNS">mechanisms</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">responsible</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">interleukin</tok> <tok cat="CD">9</tok> <tok cat="LRB">(</tok><tok cat="NN">IL-9</tok><tok cat="RRB">)</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="JJ">I-transformed</tok> <tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">C5MJ2</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">investigated</tok><cons cat="NP" ref="i2" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@35@@oe@16-12-2010
866317403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="NN">IL-9</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">controlled</tok><cons cat="NP" ref="i3" null="NONE"></cons><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">part</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
866317404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Transient</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">luciferase</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="VP"><tok cat="VBN">linked</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">serially</tok> <tok cat="VBN">deleted</tok></cons> <tok cat="NNS">sequences</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">5'-flanking</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-9</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">revealed</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="JJ">several</tok> <cons cat="NP"><tok cat="JJ">positive</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">negative</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">elements</tok></cons></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">basal</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">inducible</tok></cons></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-9</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">C5MJ2</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
866317405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="DT">An</tok> <tok cat="NN">AP-1</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="CD">-146</tok> <tok cat="TO">to</tok> <tok cat="CD">-140</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-9</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
866317406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <cons cat="NP"><tok cat="JJ">proximal</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="CD">-46</tok> <tok cat="CC">and</tok> <tok cat="CD">-80</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">minimum</tok> <tok cat="NN">sequence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">basal</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">inducible</tok></cons></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-9</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">C5MJ2</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
866317407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Within</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">region</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i6" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="CD">-59</tok> <tok cat="TO">to</tok> <tok cat="CD">-50</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">adjacent</tok> <cons cat="NP"><tok cat="JJ">20-base</tok> <tok cat="NN">pair</tok> <tok cat="JJ">upstream</tok> <tok cat="NN">sequence</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">critical</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-9</tok> <tok cat="NN">promoter</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
866317408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">DNA-protein</tok> <tok cat="NN">binding</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBD">indicated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">potentially</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">novel</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="PP"><tok cat="IN">around</tok> <cons cat="NP"><tok cat="CD">35</tok> <tok cat="NN">kDa</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">important</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
866317409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Mutations</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">this</tok> <cons cat="NP"><tok cat="JJ">proximal</tok> <tok cat="NN">promoter</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">abolished</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
866317410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">Taken</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">together</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cooperation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">different</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">IL-9</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
866323001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Mapping</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">repression</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">lymphoid-specific</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="NP"><tok cat="NN">oct-2A</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
866323002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">lymphoid-specific</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="NP"><tok cat="NN">Oct-2a</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">B</tok> <tok cat="JJ">cell-specific</tok></cons> <cons cat="ADJP"><tok cat="JJ">transcriptional</tok></cons></cons> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">octamer</tok> <tok cat="NN">motif</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@16-12-2010
866323003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Structure/function</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">various</tok> <cons cat="NP"><tok cat="NN">Oct-2a</tok> <tok cat="NN">effector</tok> <tok cat="NNS">regions</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">context</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GAL4</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Oct-2a</tok></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><tok cat="CD">two</tok> <cons cat="ADJP"><tok cat="RB">functionally</tok> <tok cat="JJ">different</tok></cons> <cons cat="NP"><tok cat="NN">activation</tok> <tok cat="NNS">domains</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">N</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">C</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><cons cat="NP" id="i2"><tok cat="NNS">termini</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
866323004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">domains</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBN">potentiated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">interactions</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">distinct</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="JJ">cell-specific</tok> <tok cat="NNS">coactivators</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
866323005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Recently</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">repression</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">N</tok> <tok cat="NN">terminus</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Oct-2a</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">amino</tok> <tok cat="NNS">acids</tok> <tok cat="CD">2-99</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
866323006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i4"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">domain</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">transferred</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potent</tok> <tok cat="NN">activator</tok></cons></cons><cons cat="ADVP" ref="i4" null="T"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">transcription</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBN">inhibited</tok><cons cat="NP" ref="i6" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
866323007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons> <cons cat="NP" id="i15" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">present</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">deletion</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">N-terminal</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Oct-2a</tok></cons></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i15" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">minimal</tok> <tok cat="NN">repression</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
866323008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">stretch</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">23</tok> <tok cat="NN">amino</tok> <tok cat="NNS">acids</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">rich</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">serine</tok> <cons cat="NP" ref="i8" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">threonine</tok> <cons cat="NP" ref="i8" null="RNR"></cons></cons><cons cat="NP" id="i8"><tok cat="NNS">residues</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">responsible</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJS">most</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">repression</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
866323009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">repression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">strongly</tok> <tok cat="JJ">dependent</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">type</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">enhancer</tok></cons> <cons cat="ADJP"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">reporter</tok> <tok cat="NN">plasmid</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons> <cons cat="VP"><tok cat="VBN">tested</tok><cons cat="NP" null="NONE"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
866323010@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">possibility</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Oct-2a</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">act</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">activator</tok></cons> <tok cat="CC">and/or</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">repressor</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">important</tok> <tok cat="NNS">consequences</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Oct-2a</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">differentiation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="JJ">developmental</tok> <tok cat="NNS">processes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
866338001@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">c-Jun</tok> <tok cat="NN">delta-domain</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><tok cat="NN">neuroendocrine</tok> <tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">sequence-</tok></cons> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">pituitary-specific</tok></cons></cons> <tok cat="NN">manner</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
866338002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i58" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">transformation</tok></cons></cons> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">governed</tok> <cons cat="NP" ref="i58" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">27-amino</tok> <tok cat="NN">acid</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">motif</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">labeled</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="DT">the</tok> <tok cat="NN">delta-domain</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i59"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i59" id="i60" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">deleted</tok> <cons cat="NP" ref="i60" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">v-Jun</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
866338003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">c-Jun</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potent</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">rat</tok> <tok cat="NN">prolactin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">rPRL</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><tok cat="DT">either</tok> <cons cat="NP"><tok cat="JJ">oncogenic</tok> <tok cat="NN">Ras</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NNS">esters</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
866338004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">characterized</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">structural</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">cell-specific</tok></cons></cons> <tok cat="NNS">requirements</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">c-Jun</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">c-Jun</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NN">response</tok></cons> <cons cat="VP"><tok cat="VBD">mapped</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">rPRL</tok> <tok cat="NN">footprint</tok> <tok cat="CD">II</tok> <tok cat="NN">repressor</tok> <tok cat="NN">site</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">pituitary-specific</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">required</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">c-Jun</tok> <tok cat="NN">delta-domain</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
866338005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">alteration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">any</tok> <tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">features</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="ADVP"><tok cat="FW">e.g.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">cis-element</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">trans-factor</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">cell-specific</tok> <tok cat="NN">background</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">switched</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">rPRL</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
866338006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">HeLa</tok> <tok cat="JJ">nonpituitary</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">c-Jun</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">activated</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">rPRL</tok> <tok cat="NN">promoter</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RBS">most</tok> <tok cat="JJ">proximal</tok></cons> <tok cat="NN">GHF-1/Pit-1</tok> <tok cat="NN">binding</tok> <tok cat="NN">site</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">footprint</tok> <tok cat="NN">I</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">synergized</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">GHF-1</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
866338007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="JJ">recombinant</tok> <tok cat="NN">GHF-1</tok></cons> <cons cat="VP"><tok cat="VBD">interacted</tok> <cons cat="ADVP"><tok cat="RB">directly</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Jun</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">c-Fos</tok></cons></cons> <tok cat="NNS">proteins</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
866338008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">important</tok> <tok cat="JJ">fundamental</tok> <tok cat="NNS">insights</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">molecular</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i61"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">c-Jun</tok> <tok cat="NN">delta-domain</tok></cons> <cons cat="VP"><tok cat="VBZ">functions</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">modulatory</tok> <tok cat="NN">switch</tok></cons></cons> <cons cat="PP" ref="i61" null="T"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">further</tok></cons> <tok cat="VBP">imply</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i62" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">functional</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">dictated</tok> <cons cat="NP" ref="i62" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="JJ">cell-specific</tok> <tok cat="NNS">influences</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">delta-domain</tok> <tok cat="NN">motif</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
866454701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">BSAP</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Pax-5</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">development</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@11@@oe@16-12-2010
866454702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">hierarchy</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">development</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="VBN">targeted</tok> <tok cat="NN">gene</tok> <tok cat="NN">inactivation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mouse</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@16-12-2010
866454703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">manner</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">paired</tok> <tok cat="NN">box</tok> <tok cat="VBG">containing</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="NP"><tok cat="NN">Pax-5</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="JJ">specific</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="NP"><tok cat="NN">BSAP</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">key</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">B</tok> <tok cat="NN">lymphopoiesis</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
866454704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Other</tok> <tok cat="JJ">experimental</tok> <tok cat="NNS">strategies</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP"><tok cat="NN">BSAP</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">proliferation</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">isotype</tok> <tok cat="NN">switching</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">immunoglobulin</tok> <tok cat="NN">heavy-chain</tok></cons> <tok cat="NN">gene</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">late</tok> <tok cat="NNS">stages</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
866678301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Modulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IFN-gamma</tok> <tok cat="NN">receptor</tok> <tok cat="NN">beta-chain</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">controls</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">responsiveness</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
866678302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IFN-gamma</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">potent</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">antiproliferative</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">apoptotic</tok></cons></cons> <tok cat="NNS">effects</tok></cons> <cons cat="SBAR" ref="i14" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="SBAR" id="i14"><cons cat="WHNP" id="i15"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i15" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">determining</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">development</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="VBN">polarized</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
866678303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">any</tok> <tok cat="NN">event</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i16"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i16" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">enables</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">become</tok> <cons cat="ADJP" role="PRD"><tok cat="RBR">less</tok> <tok cat="JJ">responsive</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IFN- gamma</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="ADVP"><tok cat="RB">potentially</tok></cons> <cons cat="VP"><tok cat="VB">alter</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">immune</tok> <tok cat="NN">responsiveness</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">Ag</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
866678304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">work</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i17"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i17" id="i18" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" ref="i18" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">TCR</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">expanded</tok> <cons cat="NP" ref="i18" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unresponsive</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i19" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">determined</tok> <cons cat="NP" ref="i19" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">lack</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">jak</tok> <tok cat="NNS">kinases</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">STAT1(alpha)</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">signal</tok> <tok cat="NN">transducer</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@52@@oe@16-12-2010
866678305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i20" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">nonresponsiveness</tok></cons> <cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="PP"><tok cat="IN">because</tok> <tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">lack</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">beta- chain</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">accessory</tok> <tok cat="NN">factor</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IFN-gamma</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">while</tok> <cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="VP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NN">time</tok></cons></cons> <cons cat="NP" ref="i20" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">maintaining</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha-chain</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
866678306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i21" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">beta-chain</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">restored</tok> <cons cat="NP" ref="i21" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="JJ">secondary</tok> <tok cat="NN">TCR</tok> <tok cat="NN">ligation</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">PMA</tok> <tok cat="NN">treatment</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
866678307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">blasts</tok></cons> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NNS">PMA</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">responsive</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
866678308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i24"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" ref="i25" id="i23" role="SBJ" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">freshly</tok></cons> <cons cat="VP"><tok cat="VBN">isolated</tok><cons cat="NP" ref="i23" null="NONE"></cons><cons cat="ADVP" ref="i24" null="T"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i22" role="SBJ"><cons cat="ADJP"><cons cat="ADJP"><tok cat="RB">highly</tok> <tok cat="VBN">enriched</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="QP"><tok cat="JJR">&gt;</tok><tok cat="CD">98</tok></cons><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" ref="i22" id="i25" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok> <tok cat="NN">responsiveness</tok></cons></cons></cons></cons><tok cat="COLON">;</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">respond</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">express</tok> <cons cat="NP"><tok cat="NN">beta-chain</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@29@@oe@16-12-2010
866678309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">progress</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">TCR</tok> <tok cat="NN">activation</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="JJ">IL-2-dependent</tok> <tok cat="NN">proliferation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i26" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">followed</tok> <cons cat="NP" ref="i26" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">secondary</tok> <tok cat="NN">TCR</tok> <tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">responsiveness</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">varies</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">participate</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">ongoing</tok> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
866679501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">Induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CIITA</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">modification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <cons cat="NP"><tok cat="NN">HLA-DR</tok> <tok cat="NN">promoter</tok></cons> <tok cat="NN">occupancy</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="JJ">thymic</tok> <tok cat="JJ">epithelial</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons></cons></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">similarities</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">distinctions</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">respect</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">HLA-DR-constitutive</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@30@@oe@16-12-2010
866679502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NNS">cultures</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">thymic</tok> <tok cat="JJ">epithelial</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TEC</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok><cons cat="NP" ref="i1" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@26@@oe@16-12-2010
866679503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">system</tok></cons> <cons cat="VP"><tok cat="VBZ">offers</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">advantage</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok></cons> <tok cat="NN">induction</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">studied</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="LQT">"</tok><tok cat="JJ">physiologic</tok><tok cat="RQT">"</tok> <tok cat="NN">cell</tok> <tok cat="NN">lineage</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">that</tok></cons><cons cat="S"><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">result</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">thymus</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" ref="i3" id="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">thought</tok> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">participate</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">selection</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">maturation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@16-12-2010
866679504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="FW">de</tok> <tok cat="FW">novo</tok></cons> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">gene</tok></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CIITA</tok> <tok cat="NN">trans-activator</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">crucial</tok> <cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">gene</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
866679505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">anatomy</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">DRA</tok> <tok cat="NN">promoter</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">corresponding</tok> <cons cat="NP"><tok cat="VBG">binding</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <cons cat="NP"><tok cat="NN">DNAse</tok> <tok cat="NN">I</tok></cons> <tok cat="NN">footprint</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
866679506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">occupancy</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">DRA</tok> <tok cat="NN">gene</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">sequences</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">nuclear</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
866679507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="VBG">resulting</tok> <tok cat="NN">occupancy</tok></cons> <cons cat="VP"><tok cat="VBZ">displays</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">strong</tok> <tok cat="NNS">similarities</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">one</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="JJ">II-constitutive</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">represented</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><tok cat="CC">both</tok> <tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Burkitt</tok> <tok cat="NN">lymphoma</tok> <tok cat="NN">line</tok> <tok cat="NN">Raji</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="JJ">tonsil- derived</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
866679508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i7" role="SBJ"><tok cat="DT">some</tok> <tok cat="JJ">peculiar</tok> <tok cat="NNS">differences</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TEC</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP" syn="COOD"><tok cat="CC">either</tok> <cons cat="ADJP"><tok cat="JJ">IFN-gamma-induced</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="RB">not</tok><cons cat="ADJP" null="QSTN"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">constitutive</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
866679509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">common</tok> <tok cat="NNS">mechanisms</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">one</tok></cons> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CIITA</tok> <tok cat="NN">trans-activator</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">distinct</tok> <tok cat="JJ">tissue-specific</tok></cons> <tok cat="NNS">constraints</tok></cons></cons> <cons cat="VP"><tok cat="VBP">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">constitutive</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">IFN-gamma-induced</tok></cons></cons> <cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
866681201@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Antisense</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">vitamin</tok> <tok cat="NN">D</tok> <tok cat="NN">receptor</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">monoblastoid</tok> <tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@16-12-2010
866681202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">active</tok> <tok cat="NN">vitamin</tok> <tok cat="NN">D3</tok> <tok cat="NN">metabolite</tok> <tok cat="NN">1,25-dihydroxycholecalciferol</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">1,25(OH)2D3</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">acts</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">antiproliferative</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="VBG">differentiating</tok></cons></cons> <tok cat="NN">agent</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">monoblastoid</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">U937</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">important</tok></cons> <cons cat="ADJP"><tok cat="JJ">immunologic</tok></cons></cons> <tok cat="NN">mediator</tok></cons> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><cons cat="ADVP"><tok cat="RB">particularly</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">belonging</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">monocyte/macrophage</tok> <tok cat="NN">lineage</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@41@@oe@16-12-2010
866681203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">effects</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">controlled</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">vitamin</tok> <tok cat="NN">D</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">VDR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">member</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">steroid</tok> <tok cat="NN">hormone</tok> <tok cat="NN">receptor</tok> <tok cat="NN">family</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
866681204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">objective</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="S" role="PRD"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">develop</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">U937</tok></cons> <tok cat="NNS">transfectants</tok></cons> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP"><tok cat="JJ">antisense</tok> <cons cat="NP"><tok cat="NN">VDR</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">use</tok> <cons cat="NP"><tok cat="DT">these</tok></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">examine</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">1,25(OH)2D3-VDR</tok> <tok cat="NN">interaction</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">lineage</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
866681205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <cons cat="NP"><tok cat="JJ">2-kb</tok> <tok cat="NN">VDR</tok> <tok cat="NN">cDNA</tok> <tok cat="NN">insert</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">complete</tok> <cons cat="NP"><tok cat="NN">VDR</tok> <tok cat="NN">coding</tok> <tok cat="NN">region</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">cloned</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">antisense</tok> <tok cat="NN">orientation</tok></cons></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">EBV</tok> <tok cat="NN">episomal</tok> <tok cat="NN">vector</tok> <tok cat="NN">pMEP4</tok></cons></cons> <cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">inducible</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">transfected</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="NN">U937</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
866681206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">resultant</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">DH42</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="NN">hygromycin</tok> <tok cat="JJ">resistant</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP"><tok cat="NN">VDR</tok> <tok cat="NN">cDNA</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJR">fewer</tok> <cons cat="NP"><tok cat="NNS">VDRs</tok></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">than</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">controls</tok></cons><cons cat="VP" null="QSTN"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">substantial</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">antiproliferative</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">1,25(OH)2D3</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
866681207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">1,25(OH)2D3</tok></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">macrophage</tok> <tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <tok cat="NN">Ags</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CD14</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CD11b</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
866681208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">subpopulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJR">smaller</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">express</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">differentiation</tok> <tok cat="NNS">markers</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">cadmium</tok> <tok cat="NN">stimulation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
866681209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Cell</tok> <tok cat="NN">cycle</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">shifts</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">distribution</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">G1</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">S</tok> <tok cat="NN">phase</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="RBR">more</tok> <tok cat="JJ">pronounced</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">cadmium</tok> <tok cat="NN">treatment</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
866681210@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">considerable</tok> <tok cat="NN">proportion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="PP" role="PRD"><tok cat="IN">outside</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cycle</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">DNA</tok> <tok cat="NN">fragmentation</tok></cons> <cons cat="VP"><tok cat="VBD">confirmed</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
866681211@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">functional</tok> <tok cat="NN">outcome</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">VDR</tok></cons> <tok cat="JJ">antisense</tok> <tok cat="NN">transfection</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">myelomonocytic</tok> <tok cat="NN">lineage</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">VDR</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">act</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">protective</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="VBN">programmed</tok> <tok cat="NN">cell</tok> <tok cat="NN">death</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
866681601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Opposing</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">rate</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">neutrophilic</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">eosinophilic</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><cons cat="NP" id="i1"><tok cat="NNS">granulocytes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
866681602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NNS">Eosinophils</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">neutrophils</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">closely</tok> <tok cat="JJ">related</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="RB">terminally</tok> <tok cat="VBN">differentiated</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <cons cat="VP"><tok cat="VBP">undergo</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">constitutive</tok> <tok cat="NN">cell</tok> <tok cat="NN">death</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@16-12-2010
866681603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">onset</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">delayed</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NNS">hemopoietins</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NNS">mediators</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
866681604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="NP" role="PRD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">reports</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBG">demonstrating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">particular</tok></cons></cons> <cons cat="NP"><tok cat="NN">dexamethasone</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBP">antagonize</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">eosinophil</tok> <tok cat="JJ">life-prolonging</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">hemopoietins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="JJ">direct</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">dexamethasone</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">eosinophil</tok> <tok cat="NN">apoptosis</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">documented</tok><cons cat="NP" ref="i4" null="NONE"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
866681605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">direct</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">eosinophil</tok></cons> <cons cat="NP" ref="i5" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">neutrophil</tok></cons> <cons cat="NP" ref="i5" null="RNR"></cons></cons><cons cat="NP" id="i5"><tok cat="NN">apoptosis</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">light</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <cons cat="ADJP"><tok cat="JJ">common</tok> <tok cat="JJ">therapeutic</tok></cons> <tok cat="NN">use</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">anti-inflammatory</tok> <cons cat="NP" ref="i6" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">anti-allergic/hypereosinophilic</tok> <cons cat="NP" ref="i6" null="RNR"></cons></cons><cons cat="NP" id="i6"><tok cat="NNS">agents</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
866681606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">dexamethasone</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">eosinophil</tok></cons> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
866681607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">dexamethasone</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potent</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">neutrophil</tok></cons> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
866681608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">dexamethasone</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="FW">i.e.</tok></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">abolished</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons> <tok cat="NN">antagonist</tok></cons> <cons cat="NP"><tok cat="NN">RU38486</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
866681609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NN">description</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">agent</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" id="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">promotes</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">eosinophil</tok></cons> <tok cat="NN">apoptosis</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inhibiting</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">neutrophil</tok></cons> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">presents</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="NN">approach</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">study</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="ADJP"><tok cat="RB">closely</tok> <tok cat="JJ">related</tok></cons> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="VP"><tok cat="VBZ">increases</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">our</tok> <tok cat="NN">understanding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">clinical</tok> <tok cat="NN">action</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">inflammation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@16-12-2010
866821301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i73" role="SBJ"><cons cat="NP"><tok cat="JJ">Recombinant</tok> <tok cat="NN">NFAT1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NFATp</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i73" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">calcineurin</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">mediates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NN">cytokine</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
866821302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NFAT</tok> <tok cat="NN">family</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">key</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NNS">genes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
866821303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">new</tok> <tok cat="NNS">isoforms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">NFAT1</tok></cons> <tok cat="LRB">(</tok><cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <tok cat="VBN">termed</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NN">NFATp</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i74"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i74" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">predominant</tok> <tok cat="NNS">isoforms</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">murine</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">human</tok></cons></cons> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
866821304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i75"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" ref="i107" id="i77" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i77" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons><cons cat="ADVP" ref="i75" null="T"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i76" role="SBJ"><tok cat="JJ">recombinant</tok> <tok cat="NN">NFAT1</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok><cons cat="NP" ref="i76" id="i79" null="NONE"></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" ref="i79" id="i80" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">expected</tok><cons cat="NP" ref="i80" id="i107" null="NONE"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="JJ">calmodulin-dependent</tok> <tok cat="NN">phosphatase</tok> <tok cat="NN">calcineurin</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i81" role="SBJ"><tok cat="PRPP">its</tok> <tok cat="NN">function</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i81" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="JJ">immunosuppressive</tok> <tok cat="NN">agent</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cyclosporin</tok> <tok cat="NN">A</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CsA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
866821305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Transactivation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">recombinant</tok> <tok cat="NN">NFAT1</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">dual</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ionomycin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">12-myristate</tok> <tok cat="NN">13-acetate</tok></cons></cons></cons></cons></cons><tok cat="COLON">;</tok></cons> <cons cat="S"><cons cat="NP" id="i82" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">potentiated</tok> <cons cat="NP" ref="i82" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">coexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">constitutively</tok> <tok cat="JJ">active</tok></cons> <tok cat="NN">calcineurin</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i82" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">CsA</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@34@@oe@16-12-2010
866821306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Immunocytochemical</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBZ">indicates</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">recombinant</tok> <tok cat="NN">NFAT1</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">localizes</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytoplasm</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">transiently</tok> <tok cat="VBN">transfected</tok></cons> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">translocates</tok> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">CsA-sensitive</tok> <tok cat="NN">manner</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="VBG">following</tok> <cons cat="NP"><tok cat="NN">ionomycin</tok> <tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
866821307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i84"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" ref="i85" id="i86" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i86" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">COS</tok> <tok cat="NNS">cells</tok></cons></cons><cons cat="ADVP" ref="i84" null="T"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i85" role="SBJ"><tok cat="NN">NFAT1</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transactivation</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" id="i87" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i87" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">correctly</tok></cons></cons></cons></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" id="i88" role="SBJ"><tok cat="PRPP">its</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">subcellular</tok> <tok cat="NN">localization</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">function</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">affected</tok> <cons cat="NP" ref="i88" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">COS</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ionomycin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">12-myristate</tok> <tok cat="NN">13-acetate</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@43@@oe@16-12-2010
866821308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Recombinant</tok> <tok cat="NN">NFAT1</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">mediate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">interleukin-2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">interleukin-4</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">alpha</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">granulocyte-macrophage</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok></cons></cons> <tok cat="NNS">promoters</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NFAT1</tok></cons> <cons cat="VP"><tok cat="VBZ">contributes</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">CsA-sensitive</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
867026901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">An</tok> <cons cat="ADJP"><tok cat="RB">alternatively</tok> <tok cat="VBN">spliced</tok></cons> <tok cat="NN">isoform</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Spi-B</tok></cons> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@10@@oe@16-12-2010
867026902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="NN">Spi-B</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="UCP" syn="COOD"><cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">Ets</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">related</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">oncoprotein</tok></cons> <cons cat="NP"><tok cat="NN">Spi-1/PU.1</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">highly</tok></cons> <tok cat="VBN">expressed</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="JJ">lymphoid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
867026903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">Ets</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="VP"><tok cat="VBP">share</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="VBN">conserved</tok> <cons cat="NP"><tok cat="NN">Ets</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">mediates</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
867026904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Spi-B</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">core</tok> <tok cat="NN">5'-GGAA-3'</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">motif</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
867026905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">Up</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">date</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">biological</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Spi-B</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">remains</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unknown</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
867026906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">describe</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">characterization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP"><tok cat="RB">alternatively</tok> <tok cat="VBN">spliced</tok></cons> <tok cat="NN">variant</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Spi-B</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">named</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NN">deltaSpi-B</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">lost</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Ets</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
867026907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="JJ">lymphoid</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">deltaspi-B</tok> <cons cat="NP" ref="i4" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">spi-B</tok> <cons cat="NP" ref="i4" null="RNR"></cons></cons><cons cat="NP" id="i4"><tok cat="NNS">mRNAs</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok></cons> <cons cat="ADVP"><tok cat="RB">simultaneously</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">ratio</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="QP"><tok cat="IN">around</tok> <tok cat="CD">10</tok></cons><tok cat="NN">%</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
867026908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">DeltaSpi-B</tok></cons> <tok cat="NN">product</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="NP"><tok cat="NN">DNA</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">recovered</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">cytoplasmic</tok> <tok cat="JJ">cellular</tok> <tok cat="NNS">extracts</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
867026909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">raise</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">hypothesis</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">delta</tok> <tok cat="NN">Spi-B</tok></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Spi-B</tok></cons> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">recruiting</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">factors</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Spi-B</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
867089701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Multiple</tok> <tok cat="NN">p21ras</tok> <tok cat="NN">effector</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="VP"><tok cat="VBP">regulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
867089702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i66" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Nuclear</tok> <tok cat="NN">Factor</tok> <tok cat="IN">of</tok> <tok cat="VBN">Activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NFAT</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">major</tok> <tok cat="NN">target</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p21ras</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">calcium</tok></cons></cons> <tok cat="NN">signalling</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i66" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">p21ras</tok> <tok cat="NNS">signals</tok></cons> <cons cat="VP"><tok cat="VBG">acting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">synergy</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">calcium/calcineurin</tok> <tok cat="NNS">signals</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
867089703@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="CD">One</tok> <tok cat="NN">p21ras</tok> <tok cat="NN">effector</tok> <tok cat="NN">pathway</tok></cons> <cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">MAP</tok> <tok cat="NN">kinase</tok> <tok cat="NN">ERK-2</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">NFAT</tok> <tok cat="NN">regulation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
867089704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">dominant</tok> <tok cat="JJ">negative</tok> <tok cat="NN">MAPKK-1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">prevents</tok> <cons cat="NP"><tok cat="NN">NFAT</tok> <tok cat="NN">induction</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
867089705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i67" role="SBJ"><cons cat="ADJP"><tok cat="RB">Constitutively</tok> <tok cat="JJ">active</tok></cons> <tok cat="NN">MAPKK-1</tok></cons> <cons cat="ADVP"><tok cat="RB">fully</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ERK-2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">Elk-1</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">substitute</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">p21ras</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">synergize</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">calcium/calcineurin</tok> <tok cat="NNS">signals</tok></cons></cons> <cons cat="S"><cons cat="NP" ref="i67" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><tok cat="NN">NFAT</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
867089706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i68" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">dominant</tok> <tok cat="JJ">negative</tok> <tok cat="NN">N17Rac</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">prevents</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">TCR</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p21ras</tok></cons></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFAT</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="S"><cons cat="NP" ref="i68" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">interfering</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ERK-2</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
867089707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i69" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NFAT</tok> <tok cat="NN">binding</tok> <tok cat="NN">site</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i69" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">complex</tok></cons> <cons cat="VP"><tok cat="VBG">comprising</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">member</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NFAT</tok> <tok cat="NN">group</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="NN">family</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
867089708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">p21ras</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP"><tok cat="NN">Rac-1</tok> <tok cat="NN">function</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
867089709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i70" role="SBJ"><tok cat="VBN">Activated</tok> <tok cat="NN">Rac-1</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">mimic</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">p21ras</tok></cons> <cons cat="S"><cons cat="NP" ref="i70" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="RB">not</tok> <tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><tok cat="NN">NFAT</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
867089710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i71" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">MAPKK-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Rac-1</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <tok cat="RB">not</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">substitute</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">p21ras</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">synergize</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">calcium</tok> <tok cat="NNS">signals</tok></cons></cons> <cons cat="S"><cons cat="NP" ref="i71" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><tok cat="NN">NFAT</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
867089711@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">p21ras</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFAT</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">multiple</tok> <tok cat="NN">effector</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">MAPKK-1/ERK-2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Rac-1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
867522801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Interleukin</tok> <tok cat="CD">10</tok></cons> <tok cat="JJ">induced</tok> <cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">divergent</tok> <tok cat="NN">protein</tok> <tok cat="NNS">kinases</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@16-12-2010
867522802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-10</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potent</tok> <tok cat="NN">mediator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NN">cell</tok></cons> <tok cat="NN">growth</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">plasma</tok> <tok cat="NN">cell</tok> <tok cat="NN">formation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
867522803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">poorly</tok></cons> <cons cat="VP"><tok cat="VBN">understood</tok><cons cat="NP" ref="i1" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
867522804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons> <cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">protooncogene</tok> <tok cat="NN">c-fos</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">investigated</tok><cons cat="NP" ref="i2" null="NONE"></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">because</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Fos</tok></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potential</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <cons cat="NP"><tok cat="NN">proliferation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
867522805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">purified</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">buffy</tok> <tok cat="NN">coat</tok> <tok cat="NNS">preparations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NN">blood</tok> <tok cat="NNS">donors</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">positive</tok> <tok cat="NN">selection</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">anti</tok> <tok cat="NN">CD20</tok> <tok cat="JJ">monoclonal</tok> <tok cat="NN">antibody</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">MiniMACS</tok> <tok cat="NN">separation</tok> <tok cat="NN">unit</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
867522806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">prestimulated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">SAC</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="CD">48</tok> <tok cat="NNS">hrs</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
867522807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Then</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">incubated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">medium</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-10</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">100</tok> <tok cat="NN">ng/ml</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="QP"><tok cat="CD">10</tok> <tok cat="TO">to</tok> <tok cat="CD">120</tok></cons> <tok cat="NN">min</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
867522808@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">RNA</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">extracted</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">phenol/chloroform</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">PCR</tok> <tok cat="JJ">assisted</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">assay</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
867522809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">significant</tok></cons> <cons cat="ADJP"><cons cat="QP"><tok cat="CD">2-4</tok></cons> <tok cat="JJ">fold</tok></cons></cons> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">30</tok> <tok cat="NN">min</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">p</tok></cons> <cons cat="ADJP"><tok cat="JJR">&lt;</tok> <cons cat="NP"><tok cat="CD">0.01</tok></cons></cons></cons><tok cat="RRB">)</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
867522810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">After</tok> <cons cat="NP"><tok cat="CD">2</tok> <tok cat="NNS">hrs</tok></cons></cons> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">declined</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">basal</tok> <tok cat="NNS">levels</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
867522811@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">dose-dependent</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">maximum</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">100</tok> <tok cat="NN">ng/ml</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
867522812@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">polymyxin</tok> <tok cat="NN">B</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
867522813@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok> <tok cat="NN">inhibitor</tok> <tok cat="NN">genistein</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">10</tok> <tok cat="NN">microM</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">complete</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-10</tok></cons> <tok cat="JJ">induced</tok> <cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok><cons cat="NP" ref="i10" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
867522814@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">H-7</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">10</tok> <tok cat="NN">microM</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">specific</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">serine/threonine</tok> <tok cat="NNS">kinases</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP"><tok cat="VBD">blocked</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-10</tok></cons> <tok cat="JJ">mediated</tok> <cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">p</tok></cons> <cons cat="ADJP"><tok cat="JJR">&lt;</tok> <cons cat="NP"><tok cat="CD">0.05</tok></cons></cons></cons><tok cat="RRB">)</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
867522815@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">conclusion</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-10</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">B-cells</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">tyrosine</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">serine/threonine</tok> <tok cat="NNS">kinases</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
867522816@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Since</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NN">report</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-10</tok></cons> <tok cat="JJ">induced</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">help</tok> <cons cat="S"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">identify</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">intracellular</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i11"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-10</tok></cons> <cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">B-cells</tok></cons><cons cat="PP" ref="i11" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
867558401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">Glucocorticoids</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">interferon-alpha</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">acquired</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">syndrome</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
867558402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">Some</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">acquired</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">syndrome</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AIDS</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">develop</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">resistance</tok></cons> <cons cat="VP"><tok cat="VBN">characterized</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="JJ">low</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">receptor</tok> <tok cat="NN">affinity</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Kd</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">mononuclear</tok><tok cat="COMMA">,</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">values</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ACTH</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">cortisol</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
867558403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">As</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">glucocorticoids</tok></cons> <cons cat="VP"><tok cat="VBP">regulate</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interferon-alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IFN</tok> <tok cat="NN">alpha</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">production</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i54" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">hypothesized</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i55" role="SBJ"><cons cat="NP"><tok cat="NN">IFN</tok> <tok cat="NN">alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cytokine</tok></cons> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="RB">predominantly</tok> <tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NNS">monocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">AIDS</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="MD">should</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">increased</tok> <cons cat="NP" ref="i55" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">cortisol-resistant</tok> <tok cat="NN">AIDS</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" ref="i54" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">attributing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">lack</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cortisol</tok> <tok cat="NN">inhibition</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IFN</tok> <tok cat="NN">alpha</tok> <tok cat="NN">production</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
867558404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok> <tok cat="NNS">characteristics</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">[3H]dexamethasone</tok> <tok cat="NN">binding</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">measured</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IFN</tok> <tok cat="NN">alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">cortisol</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">ACTH</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">AIDS</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP" id="i57"><tok cat="IN">with</tok> <cons cat="NP" ref="i56" null="RNR"></cons></cons></cons><cons cat="PRN" id="i58"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AIDS-GR</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons map="i57" cat="PP"><tok cat="IN">without</tok> <cons cat="NP" ref="i56" null="RNR"></cons></cons><cons map="i58" cat="PRN" ref="i59" null="ICH"></cons></cons></cons><cons cat="NP" id="i56"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">resistance</tok></cons> <cons cat="PRN" id="i59"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AIDS-C</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">controls</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">C</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
867558405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Monocytes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AIDS-GR</tok> <tok cat="NNS">patients</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">receptor</tok> <tok cat="NN">Kd</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="QP"><tok cat="CD">10.5</tok> <tok cat="CC">+/-</tok> <tok cat="CD">4.2</tok></cons> <tok cat="NN">nmol/L</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i60"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i60" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="JJR">higher</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" id="i62"><tok cat="DT">the</tok> <tok cat="NN">AIDS-C</tok> <tok cat="NN">group</tok></cons></cons></cons> <tok cat="LRB">(</tok><cons cat="NP" id="i65"><cons cat="QP"><tok cat="CD">2.9</tok> <tok cat="CC">+/-</tok> <tok cat="CD">0.8</tok></cons> <tok cat="NN">nmol/L</tok></cons><tok cat="RRB">)</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons map="i62" cat="NP"><tok cat="JJ">normal</tok> <tok cat="NNS">subjects</tok></cons> <tok cat="LRB">(</tok><cons map="i65" cat="NP"><cons cat="QP"><tok cat="CD">2.0</tok> <tok cat="CC">+/-</tok> <tok cat="CD">0.8</tok></cons> <tok cat="NN">nmol/L</tok></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="PRN"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJR">&lt;</tok> <tok cat="CD">0.01</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
867558406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i64" role="SBJ"><tok cat="NN">IFN</tok> <tok cat="NN">alpha</tok> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">increased</tok> <cons cat="NP" ref="i64" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">AIDS-GR</tok> <tok cat="NN">group</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="QP"><tok cat="CD">17</tok> <tok cat="CC">+/-</tok> <tok cat="CD">6</tok></cons></cons> <tok cat="CC">vs.</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">4</tok> <tok cat="CC">+/-</tok> <tok cat="CD">1</tok></cons> <tok cat="NN">U/mL</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">AIDS-C</tok> <tok cat="NN">group</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">2</tok> <tok cat="CC">+/-</tok> <tok cat="CD">0.5</tok></cons> <tok cat="NN">U/mL</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">C</tok> <tok cat="NN">group</tok></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJR">&lt;</tok> <tok cat="CD">0.01</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
867558407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i63" role="SBJ"><tok cat="NNS">Correlations</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" ref="i63" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">plasma</tok> <tok cat="NN">IFN</tok> <tok cat="NN">alpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">receptor</tok> <tok cat="NN">Kd</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AIDS-GR</tok></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">r</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">0.77</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IFN</tok> <tok cat="NN">alpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">plasma</tok> <tok cat="NN">cortisol</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NN">group</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">r</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">0.74</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
867558408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i66" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">poly(I)-poly(C)-induced</tok> <tok cat="NN">IFN</tok> <tok cat="NN">alpha</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i66" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NNS">glucocorticoids</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">C</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">AIDS-C</tok></cons></cons> <tok cat="NNS">groups</tok></cons></cons></cons> <tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="RB">approximately</tok> <tok cat="CD">80</tok></cons><tok cat="NN">%</tok></cons> <cons cat="NP"><tok cat="NN">inhibition</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">groups</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COLON">;</tok></cons> <cons cat="S"><cons cat="NP" id="i67" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">reversed</tok> <cons cat="NP" ref="i67" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="NN">receptor</tok> <tok cat="NN">antagonist</tok> <tok cat="NN">RU-38486</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@37@@oe@16-12-2010
867558409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i68" role="SBJ"><tok cat="NNS">glucocorticoids</tok></cons> <cons cat="VP"><tok cat="VBD">failed</tok> <cons cat="S"><cons cat="NP" ref="i68" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IFNalpha</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">AIDS-GR</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons> <tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="RB">approximately</tok> <tok cat="CD">20</tok></cons><tok cat="NN">%</tok></cons> <cons cat="NP"><tok cat="NN">inhibition</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
867558410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">conclusion</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">elevated</tok> <tok cat="NN">IFN</tok> <tok cat="NN">alpha</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">AIDS-GR</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="PP" role="PRD"><tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">lack</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">inhibitory</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cortisol</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">IFN</tok> <tok cat="NN">alpha</tok> <tok cat="NN">production</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cortisol</tok> <tok cat="NN">resistance</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
867607201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Binding</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">cooperative</tok> <tok cat="NNS">interactions</tok></cons></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="CD">two</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="JJ">cell-specific</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NNS">coactivators</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@11@@oe@16-12-2010
867607202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">transactivator</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CIITA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="JJ">octamer-binding</tok> <tok cat="NN">protein</tok> <tok cat="JJ">1/octamer-binding</tok> <tok cat="NN">factor</tok> <tok cat="NN">1/Oct</tok> <tok cat="NN">coactivator</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Bob1/OBF-1/OCA-B</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">represent</tok> <cons cat="NP"><tok cat="CD">two</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="JJ">cell-specific</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NNS">coactivators</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@29@@oe@16-12-2010
867607203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">CIITA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Bob1</tok></cons></cons> <cons cat="VP"><tok cat="VBP">interact</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">proteins</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="VBN">conserved</tok> <tok cat="JJ">upstream</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">promoters</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="JJ">major</tok> <tok cat="NN">histocompatibility</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">octamer-binding</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Oct-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Oct-2</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
867607204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">Both</tok> <cons cat="NP"><tok cat="NN">CIITA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Bob1</tok></cons></cons> <cons cat="VP"><tok cat="VBP">increase</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">DRA</tok> <tok cat="NN">promoter</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <tok cat="JJ">prototypic</tok> <cons cat="NP"><tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
867607205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CIITA</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">interactions</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NNS">promoters</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Bob1</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">independent</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">octamer-binding</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
867607206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <cons cat="NP" ref="i4" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <cons cat="NP" ref="i4" null="RNR"></cons></cons><cons cat="NP" id="i4"><tok cat="NN">binding</tok> <tok cat="NNS">assays</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">confirm</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Bob1</tok></cons> <cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">CIITA</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
867607207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">CIITA</tok></cons> <cons cat="VP" syn="COOD"><cons cat="CONJP"><tok cat="RB">not</tok> <tok cat="RB">only</tok></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBZ">recruits</tok> <cons cat="NP"><tok cat="DT">another</tok> <tok cat="NN">B</tok> <tok cat="JJ">cell-specific</tok> <tok cat="NN">coactivator</tok></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">increase</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NNS">promoters</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
867652101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">antigen</tok> <tok cat="CD">2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">latent</tok> <tok cat="NN">membrane</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">independently</tok></cons> <cons cat="VP"><tok cat="VBP">transactivate</tok> <cons cat="NP"><tok cat="NN">p53</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
867652102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">immortalization</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EBV</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">permanent</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cellular</tok> <tok cat="NNS">processes</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i61"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i61" role="SBJ" null="T"></cons><cons cat="VP"><cons cat="ADVP"><tok cat="RB">normally</tok></cons> <tok cat="VB">regulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cell</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">division</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">functions</tok></cons> <cons cat="VP"><tok cat="VBN">possessed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">p53</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
867652103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBN">initiated</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">evaluate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">relationships</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">EBV</tok> <tok cat="JJ">latent</tok> <tok cat="NNS">genes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p53</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i62" role="SBJ"><tok cat="NN">p53</tok> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i62" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">increase</tok> <cons cat="ADVP"><tok cat="RB">approximately</tok> <tok cat="RB">10-fold</tok></cons> <cons cat="PP" adv="TMP"><cons cat="NP"><cons cat="QP"><tok cat="CD">4</tok> <tok cat="TO">to</tok> <tok cat="CD">5</tok></cons> <tok cat="NNS">days</tok></cons> <tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">EBV</tok> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">purified</tok> <tok cat="VBG">resting</tok> <tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="VBN">induced</tok> <tok cat="NN">p53</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">transcriptionally</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">active</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@42@@oe@16-12-2010
867652104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Latent</tok> <tok cat="NN">membrane</tok> <tok cat="NN">protein</tok> <tok cat="CD">1</tok></cons> <cons cat="VP" ref="i102" null="RNR"></cons></cons><tok cat="CC">and</tok><cons cat="S"><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJR">lesser</tok> <tok cat="NN">extent</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">EBV</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">antigen</tok> <tok cat="CD">2</tok></cons> <cons cat="VP" ref="i102" null="RNR"></cons></cons></cons><cons cat="VP" id="i102"><tok cat="VBD">mediated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">p53</tok> <tok cat="NNS">levels</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
868310601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Differential</tok> <tok cat="NN">utilization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">Janus</tok> <tok cat="JJ">kinase-signal</tok> <tok cat="NN">transducer</tok></cons> <tok cat="NN">activator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">natural</tok> <tok cat="NN">killer</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-12</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IFN-alpha</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
868310602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i73" role="SBJ"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><cons cat="NP"><tok cat="NN">IL-2-</tok></cons><cons cat="ADJP" null="QSTN"></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><cons cat="NP"><tok cat="NN">IL-12-</tok></cons><cons cat="ADJP" null="QSTN"></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">IFN-alpha-mediated</tok></cons></cons> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP" ref="i73" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NK3.3</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
868310603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Gel</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">immunoprecipitation</tok></cons></cons> <tok cat="NNS">analyses</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">addition</tok> <tok cat="TO">to</tok> <cons cat="S"><cons cat="NP" ref="i74" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">activating</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">STAT3</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">transducer</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">activator</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription-3</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT5</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i74" role="SBJ"><tok cat="NN">IL-2</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">tyrosine</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">serine</tok></cons></cons> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT1</tok> <tok cat="NN">alpha</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i75"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i75" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">formed</tok> <cons cat="NP"><tok cat="JJ">IFN-gamma-activated</tok> <tok cat="NN">sequence-binding</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="PRP">itself</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">STAT3</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
868310604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IFN-alpha</tok></cons></cons> <cons cat="VP"><tok cat="VBD">activated</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT1</tok> <tok cat="NN">alpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT5</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">IL-2</tok></cons> <cons cat="ADVP"><tok cat="RB">predominantly</tok></cons> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP"><tok cat="NN">STAT5</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IFN-alpha</tok></cons> <cons cat="ADVP"><tok cat="RB">predominantly</tok></cons> <cons cat="VP"><tok cat="VBD">activated</tok> <cons cat="NP"><tok cat="NN">STAT1</tok> <tok cat="NN">alpha</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
868310605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-2</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><tok cat="RBR">less</tok> <tok cat="NN">STAT1</tok> <tok cat="NN">alpha</tok> <tok cat="NN">activation</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IFN-alpha</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJR">greater</tok> <tok cat="NN">STAT5</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">NK3.3</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="VBN">compared</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">preactivated</tok> <tok cat="JJ">primary</tok> <tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
868310606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">NK3.3</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">IL-2</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">comparable</tok> <tok cat="NN">formation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">c-fos</tok> <tok cat="NN">promoter</tok> <tok cat="JJ">sis-inducible</tok> <tok cat="NN">element</tok> <tok cat="JJ">IFN-gamma-activated</tok> <tok cat="NN">sequence-binding</tok></cons> <tok cat="NNS">complexes</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">STAT3</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">complexes</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT1</tok> <tok cat="NN">alpha</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">while</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">preactivated</tok> <tok cat="JJ">primary</tok> <tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="ADVP"><tok cat="RB">preferentially</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">complexes</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT1</tok> <tok cat="NN">alpha</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
868310607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">signaling</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">NK3.3</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">always</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">identical</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
868310608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <tok cat="NN">contrast</tok> <tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IFN-alpha</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">IL-12</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">strong</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT4</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">variable</tok> <tok cat="JJ">weak</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT3</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
868310609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">supershift</tok> <tok cat="NNS">analyses</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">c-fos</tok> <tok cat="NN">promoter</tok> <tok cat="JJ">sis-inducible</tok> <tok cat="NN">element</tok> <tok cat="NN">probe</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-12</tok></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">activated</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT4</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">STAT1</tok> <tok cat="NN">alpha</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT3</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">complexes</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">STAT4</tok></cons> <cons cat="ADVP"><tok cat="RB">only</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">STAT4</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">STAT1</tok> <tok cat="NN">alpha</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">STAT3</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">STAT1</tok> <tok cat="NN">alpha</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">STAT1</tok> <tok cat="NN">alpha</tok></cons> <cons cat="ADVP"><tok cat="RB">only</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">preactivated</tok> <tok cat="JJ">primary</tok> <tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@50@@oe@16-12-2010
868310610@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">STAT1</tok> <tok cat="NN">alpha</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">IL-12</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">correlated</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">serine</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">tyrosine</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
868310611@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">IL-2</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">JAK1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">JAK3</tok></cons></cons></cons></cons><cons cat="PP" ref="i101" null="RNR"></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-12</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">JAK2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TYK2</tok></cons></cons></cons></cons> <cons cat="PP" ref="i101" null="RNR"></cons></cons><cons cat="PP" id="i101"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="JJ">preactivated</tok> <tok cat="JJ">primary</tok> <tok cat="NN">NK</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NK3.3</tok></cons></cons> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
868310612@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">Differential</tok> <tok cat="NN">phosphorylation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">consequent</tok> <tok cat="JJ">differential</tok> <tok cat="NN">activation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADJP"><tok cat="JJ">separate</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">overlapping</tok></cons></cons> <tok cat="NN">STAT</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-12</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IFN-alpha</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">basis</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">similarities</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">differences</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">actions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cytokines</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
868311001@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Sublethal</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">oxidative</tok> <tok cat="NN">stress</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">stimulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-jun</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">suppress</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
868311002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i79" role="SBJ"><cons cat="NP"><tok cat="JJ">Sublethal</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">oxidative</tok> <tok cat="NN">stress</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">well</tok></cons> <tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" ref="i79" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">alter</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="JJ">functional</tok> <tok cat="NNS">responses</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">underlying</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unknown</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
868311003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">current</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">oxidative</tok> <tok cat="NN">stress</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NNS">activities</tok></cons> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">c-Fos/c-Jun</tok> <tok cat="NN">AP-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-AT</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
868311004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">present</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">acutely</tok></cons> <tok cat="VBN">exposed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">micromolar</tok> <tok cat="NNS">concentrations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">H2O2</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">exhibit</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">substantial</tok> <tok cat="NNS">increases</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-jun</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">c-fos</tok></cons></cons> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
868311005@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">preferential</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-jun</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">H2O2</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">represent</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">redox</tok> <tok cat="NN">stabilization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">mRNA</tok> <tok cat="NNS">transcripts</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i103" role="SBJ"><tok cat="JJ">oxidative</tok> <tok cat="NNS">signals</tok></cons> <cons cat="ADVP"><tok cat="RB">closely</tok></cons> <cons cat="VP"><tok cat="VBD">resembled</tok> <cons cat="NP"><tok cat="NN">PHA/PMA</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i103" role="SBJ" null="NONE"></cons><cons cat="VP"><cons cat="ADVP"><tok cat="RB">effectively</tok></cons> <tok cat="VBG">transactivating</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">full</tok> <tok cat="NN">length</tok> <tok cat="NN">c-jun</tok> <tok cat="NN">promoter</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">proximal</tok> <tok cat="NN">jun1</tok> <cons cat="ADJP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">tumor</tok> <tok cat="JJ">promoter-responsive</tok></cons> <tok cat="NN">element</tok></cons> <tok cat="LRB">(</tok><tok cat="NN">TRE</tok><tok cat="RRB">)</tok><tok cat="JJ">-like</tok></cons> <tok cat="NN">promoter</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
868311006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Similarly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i80" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">consensus</tok> <tok cat="NN">AP-1</tok> <tok cat="NN">TRE</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">jun</tok> <tok cat="JJ">TRE-like</tok> <tok cat="NNS">motifs</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">exposed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">oxidative</tok> <tok cat="NNS">signals</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">PHA/PMA</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">indistinguishable</tok></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" ref="i80" id="i81" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">being</tok> <cons cat="VP"><tok cat="VBN">composed</tok> <cons cat="NP" ref="i81" null="NONE"></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Fos</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">JunD</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
868311007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="UCP" syn="COOD"><cons cat="NP"><tok cat="NN">PHA/PMA</tok></cons> <tok cat="CC">but</tok> <cons cat="ADJP"><tok cat="RB">not</tok> <tok cat="JJ">oxidative</tok></cons></cons> <tok cat="NNS">signals</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">coordinate</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">reporter</tok> <tok cat="NNS">constructs</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">AP-1-TRE</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons> <tok cat="NN">promoter</tok> <tok cat="NNS">regions</tok></cons></cons></cons></cons></cons> <cons cat="CONJP"><tok cat="IN">along</tok> <tok cat="IN">with</tok></cons> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
868311008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">sublethal</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">H2O2</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">actively</tok></cons> <cons cat="VP"><tok cat="VBD">suppressed</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-AT</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons> <tok cat="NNS">reporters</tok></cons></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">mRNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">PHA/PMA</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
868311009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Gel</tok> <tok cat="NN">shift</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">oxidative</tok> <tok cat="NN">suppression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">represented</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">early</tok> <tok cat="NN">generation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFAT</tok> <tok cat="NNS">complexes</tok></cons></cons></cons> <cons cat="CONJP"><tok cat="RB">rather</tok> <tok cat="IN">than</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">preformed</tok> <tok cat="NN">NF-AT</tok> <tok cat="NNS">complexes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
868311010@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">oxidative</tok> <tok cat="NNS">signals</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP" syn="COOD"><cons cat="ADVP"><tok cat="RB">positively</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">negatively</tok></cons></cons> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NNS">events</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NN">redox</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">uncouple</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-jun</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">up-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
868742901@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HIV-1</tok> <tok cat="NN">tat</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">new</tok> <cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="JJ">cell-specific</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">downregulates</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">MIP-1</tok> <tok cat="NN">alpha</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NNS">T-cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@22@@oe@16-12-2010
868742902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">MIP-1</tok> <tok cat="NN">alpha</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="VBN">secreted</tok> <cons cat="NP"><tok cat="NN">chemokine</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NN">stem</tok> <tok cat="NNS">cells</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">modulate</tok> <cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NNS">responses</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@16-12-2010
868742903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">replication</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">CD8+</tok> <tok cat="NNS">T-cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
868742904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MIP-1</tok> <tok cat="NN">alpha</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">CD4+</tok> <tok cat="NNS">T-cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
868742905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">transformed</tok> <tok cat="NNS">B-cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@16-12-2010
868742906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">new</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">family</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">MNP</tok> <tok cat="NN">family</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WPP">whose</tok> <tok cat="NN">expression</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">crucial</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">MIP-1</tok> <tok cat="NN">alpha</tok></cons> <tok cat="NN">transcription</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NN">activation</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">transformed</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
868742907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NNS">Monocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="VBN">transformed</tok> <tok cat="NNS">B-cells</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">normally</tok></cons> <cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">MNP-1</tok></cons> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">MNP-2</tok></cons> <cons cat="ADVP"><tok cat="RB">weakly</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">T-cells</tok></cons> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <cons cat="NP"><tok cat="NN">MNP-2</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
868742908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Recently</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">reported</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HIV-1</tok> <tok cat="NN">tat</tok></cons> <cons cat="VP"><tok cat="VBZ">downregulates</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">MIP-1</tok> <tok cat="NN">alpha</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">T-cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
868742909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">report</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MNP-1</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">T-cells</tok></cons> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">tat</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
868742910@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">neither</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HTLV-1</tok> <tok cat="NN">tax</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">T-cells</tok></cons></cons></cons> <tok cat="CC">nor</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">EBV</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">B-cells</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">any</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">MNP-1</tok></cons> <cons cat="NP" ref="i5" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">MNP-2</tok></cons> <cons cat="NP" ref="i5" null="RNR"></cons></cons><cons cat="NP" id="i5"><tok cat="NN">expression</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">showing</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">specific</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">tat</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
868742911@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">propose</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">HIV-1</tok> <tok cat="NN">tat</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">MIP-1</tok> <tok cat="NN">alpha</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inducing</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">MNP-1</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">T-cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP" syn="COOD"><cons cat="ADVP"><tok cat="RB">probably</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <tok cat="CC">either</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">competing</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">MNP-2</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">MIP-1</tok> <tok cat="NN">alpha</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">sequestering</tok> <cons cat="NP"><tok cat="PRP">it</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="JJ">inactive</tok> <tok cat="NNS">forms</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
869090001@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-10</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappa</tok> <tok cat="NN">B/Rel</tok></cons> <tok cat="JJ">nuclear</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">CD3-stimulated</tok> <tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@16-12-2010
869090002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-10</tok></cons> <cons cat="ADVP"><tok cat="RB">markedly</tok></cons> <cons cat="VP"><tok cat="VBZ">reduces</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <cons cat="NP"><tok cat="LRB">(</tok><tok cat="NN">NF</tok><tok cat="RRB">)</tok><tok cat="NN">-kappa</tok> <tok cat="NN">B/Rel</tok></cons> <tok cat="JJ">nuclear</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">PBMC</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">anti-CD3</tok> <tok cat="NN">mAb</tok></cons></cons> <cons cat="NP"><tok cat="NN">OKT3</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@16-12-2010
869090003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">exerted</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B/Rel</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">mAb</tok> <tok cat="NN">OKT3</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <tok cat="RB">not</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">PMA</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
869090004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">As</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">judged</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">supershifting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">DNA-protein</tok> <tok cat="NNS">complexes</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Abs</tok></cons> <cons cat="VP"><tok cat="VBG">recognizing</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NNS">components</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B/Rel</tok> <tok cat="NN">protein</tok> <tok cat="NN">family</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">p50/p65</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Rel</tok> <tok cat="NN">A</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="JJ">heterodimeric</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">primarily</tok></cons> <cons cat="VP"><tok cat="VBN">affected</tok><cons cat="NP" ref="i1" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
869090005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">maximal</tok> <tok cat="NN">effect</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons> <tok cat="NN">concentration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">20</tok> <tok cat="NN">U/ml</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
869090006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="NN">IL-10</tok></cons> <tok cat="JJ">inhibitory</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">exerted</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
869090007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Indeed</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <cons cat="VP"><tok cat="VBN">pretreated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="RB">so</tok> <cons cat="VP"><tok cat="VBP">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="JJ">nuclear</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">purified</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">OKT3</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
869090008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="JJ">Soluble</tok> <tok cat="NNS">factors</tok></cons> <cons cat="VP"><tok cat="VBP">do</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">appear</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">inhibition</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
869090009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">On</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">other</tok> <tok cat="NN">hand</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">up-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD80</tok> <tok cat="NN">Ag</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PBMC</tok></cons> <cons cat="VP"><tok cat="VBN">incubated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">OKT3</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">anti-CD28</tok> <tok cat="NN">mAb</tok></cons> <cons cat="NP"><tok cat="NN">CLB-CD28/1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">restores</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B/Rel</tok></cons> <tok cat="JJ">nuclear</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">IL-10-inhibited</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
869090010@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B/Rel</tok></cons> <tok cat="NN">inhibition</tok></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">ascribed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">lack</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cooperation</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">accessory</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">down-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">costimulatory</tok> <tok cat="NN">molecule</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD80</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">IL-10</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
869090011@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-10</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B/Rel</tok></cons> <tok cat="JJ">nuclear</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">CD3-stimulated</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
869090012@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Since</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">inappropriate</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">kappa</tok> <tok cat="JJ">B-driven</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">physiopathologic</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">diseases</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">infection</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">support</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">possibility</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">cytokine</tok></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">suppress</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">undesirable</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
869112301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">Activation</tok> <tok cat="NN">protein</tok> <tok cat="JJ">1-dependent</tok></cons> <cons cat="ADJP"><tok cat="JJ">transcriptional</tok></cons></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="CD">2</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">Ca2+/calmodulin</tok> <tok cat="NN">kinase</tok> <tok cat="NN">type</tok> <tok cat="NN">IV/Gr</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
869112302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">Ca2+/calmodulin-dependent</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="LRB">(</tok><tok cat="NN">CaMK</tok><tok cat="RRB">)</tok> <tok cat="NN">type</tok> <tok cat="NN">IV/Gr</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">selectively</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">signaling</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">antigen</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TCR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">mediates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">some</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">Ca(2+)-dependent</tok></cons> <cons cat="ADJP"><tok cat="JJ">transcriptional</tok></cons></cons> <tok cat="NNS">events</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">follow</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">TCR</tok></cons> <tok cat="NN">engagement</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@45@@oe@16-12-2010
869112303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">CaMKIV/Gr</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">activation</tok> <tok cat="NN">protein</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AP-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="UCP" syn="COOD"><cons cat="ADVP"><tok cat="RB">alone</tok></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">synergy</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">mitogens</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">p21ras</tok> <tok cat="NN">oncoprotein</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
869112304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="NN">CaMKIV/</tok> <tok cat="NN">Gr</tok></cons> <tok cat="NN">signaling</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-fos</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">independent</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p21ras</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">calcineurin</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
869112305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">AP-1</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">integral</tok> <tok cat="NN">component</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="IN">of</tok> <tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok> <tok cat="LRB">(</tok><tok cat="NN">NFAT</tok><tok cat="RRB">)</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">complex</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" id="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="CD">2</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
869112306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">CaMKIV/Gr</tok></cons> <cons cat="VP"><tok cat="VBZ">reconstitutes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">capacity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cytosolic</tok> <tok cat="NN">component</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFAT</tok></cons></cons></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">direct</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NFAT</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">non-T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
869112307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">reveal</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">central</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">CaMKIV/Gr</tok></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">Ca(2+)-regulated</tok> <tok cat="NN">activator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
869112701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">Mechanisms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells-1</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
869112702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Nuclear</tok> <tok cat="NN">factor</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NN">cells-family</tok></cons></cons></cons> <tok cat="NNS">proteins</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NFAT1/NFATp</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">NFATc</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">NFAT3</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NFAT4/NFATx/NFATc3</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBP">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">key</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NNS">genes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
869112703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">defined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">NFAT1</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
869112704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP"><tok cat="NNP">NFAT1</tok></cons> <cons cat="VP"><tok cat="VBZ">possesses</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NN">transactivation</tok> <tok cat="NNS">domains</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i57"><tok cat="WPP">whose</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="S"><cons cat="NP" ref="i57" id="i58" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">conserved</tok> <cons cat="NP" ref="i58" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">other</tok> <tok cat="NN">NFAT-family</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="VBN">conserved</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">domain</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i59"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i59" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">mediates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">recruitment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBG">cooperating</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i60"><tok cat="RB">even</tok> <tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i61" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i61" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">absence</tok> <tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">regions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons><cons cat="ADVP" ref="i60" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
869112705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i62" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">NH2-terminal</tok> <tok cat="NN">transactivation</tok> <tok cat="NN">domain</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">modulated</tok> <cons cat="NP" ref="i62" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">adjacent</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">region</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i63"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i63" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="VBN">conserved</tok> <tok cat="NN">sequence</tok> <tok cat="NNS">motifs</tok></cons> <cons cat="VP"><tok cat="VBN">represented</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="RB">only</tok> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NFAT</tok> <tok cat="NN">family</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
869112706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">emphasize</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">multiple</tok> <tok cat="NNS">levels</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i64"><tok cat="IN">at</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i65" role="SBJ"><tok cat="JJ">NFAT-dependent</tok> <tok cat="NN">transactivation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok><cons cat="NP" ref="i65" null="NONE"></cons><cons cat="PP" ref="i64" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">predict</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">significant</tok> <tok cat="NNS">differences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">architecture</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cooperative</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NN">NFAT-family</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
869113501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i67" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">Ets</tok> <tok cat="NN">protein</tok> <tok cat="NN">Spi-B</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i67" null="NONE"></cons><cons cat="PP"><tok cat="RB">exclusively</tok> <tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">development</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
869113502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Spi-B</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">PU.1</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">hematopoietic-specific</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i68"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i68" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">constitute</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">subfamily</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Ets</tok> <tok cat="NN">family</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
869113503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="JJ">contrary</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">previous</tok> <tok cat="NNS">reports</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">PU.1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Spi-B</tok></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">very</tok> <tok cat="JJ">different</tok></cons> <tok cat="NN">expression</tok> <tok cat="NNS">patterns</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
869113504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i69" role="SBJ"><tok cat="NN">PU.1</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i69" null="NONE"></cons><cons cat="PP" id="i70"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">levels</tok></cons></cons> <cons cat="PP" id="i71"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">mast</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNS">megakaryocytes</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNS">neutrophils</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">immature</tok> <tok cat="JJ">erythroid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons map="i70" cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="JJR">lower</tok> <tok cat="NNS">levels</tok></cons></cons> <cons map="i71" cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="NNS">erythrocytes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
869113505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PU.1</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">completely</tok> <tok cat="JJ">absent</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJS">most</tok> <tok cat="JJ">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="VBN">based</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">sensitive</tok> <tok cat="NN">RT-PCR</tok> <tok cat="NNS">assays</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
869113506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i72" role="SBJ"><tok cat="NN">Spi-B</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i72" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">exclusively</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">lymphoid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i72" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">early</tok> <tok cat="JJ">fetal</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">thymus</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">spleen</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
869113507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">In</tok> <tok cat="FW">situ</tok></cons> <tok cat="NNS">hybridizations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="JJ">murine</tok> <tok cat="NNS">tissues</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="NP"><tok cat="NN">Spi-B</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">medulla</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">thymus</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">white</tok> <tok cat="NN">pulp</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">spleen</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">germinal</tok> <tok cat="NNS">centers</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">lymph</tok> <tok cat="NNS">nodes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
869113508@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Spi-B</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">very</tok> <tok cat="JJ">abundant</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i75" role="SBJ"><tok cat="CC">both</tok> <tok cat="NN">Spi-B</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i75" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">some</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
869113509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">In</tok> <tok cat="FW">situ</tok></cons> <tok cat="NN">hybridization</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Northern</tok> <tok cat="NN">blot</tok> <tok cat="NN">analysis</tok></cons></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Spi-B</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">increases</tok> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">maturation</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">decreases</tok> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">maturation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
869113510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Gel-retardation</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Spi-B</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">all</tok> <tok cat="JJ">putative</tok> <tok cat="NN">PU.1</tok> <tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBP">do</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">reveal</tok> <cons cat="NP"><tok cat="DT">any</tok> <tok cat="JJ">preferred</tok> <tok cat="NN">Spi-B</tok> <tok cat="NN">binding</tok> <tok cat="NN">site</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
869113511@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i76" role="SBJ"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">PU.1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Spi-B</tok></cons></cons> <cons cat="VP"><tok cat="VBP">function</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NNS">activators</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immunoglobulin</tok> <tok cat="NN">light-chain</tok> <tok cat="NN">enhancer</tok> <tok cat="NN">E</tok> <tok cat="NN">lambda</tok> <tok cat="CD">2.4</tok></cons></cons></cons></cons> <cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i77"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" ref="i76" id="i78" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">coexpressed</tok> <cons cat="NP" ref="i78" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Pip</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">PU.1-interaction</tok> <tok cat="NN">partner</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">NIH-3T3</tok> <tok cat="NNS">cells</tok></cons></cons><cons cat="ADVP" ref="i77" null="T"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
869113512@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" ref="i79" id="i80" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">Taken</tok> <cons cat="NP" ref="i80" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">together</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i79" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">differences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patterns</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">expression</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Spi-B</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">PU.1</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">distinguish</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">each</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">development</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immune</tok> <tok cat="NN">system</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
869292401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">BCL-6</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">POZ/zinc-finger</tok> <tok cat="NN">protein</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <tok cat="JJ">sequence-specific</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">repressor</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
869292402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i43" role="SBJ"><cons cat="NP"><cons cat="QP"><tok cat="RB">Approximately</tok> <tok cat="CD">40</tok></cons><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">diffuse</tok> <tok cat="JJ">large</tok> <tok cat="NN">cell</tok> <tok cat="NN">lymphoma</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i43" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">chromosomal</tok> <tok cat="NNS">translocations</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i44"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i44" id="i45" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">deregulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">BCL6</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i45" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">juxtaposing</tok> <cons cat="NP"><tok cat="JJ">heterologous</tok> <tok cat="NNS">promoters</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">BCL-6</tok> <tok cat="NN">coding</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
869292403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">BCL6</tok> <tok cat="NN">gene</tok></cons> <cons cat="VP"><tok cat="VBZ">encodes</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">95-kDa</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="CD">six</tok> <tok cat="JJ">C-terminal</tok> <tok cat="NN">zinc-finger</tok> <tok cat="NNS">motifs</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">N-terminal</tok> <tok cat="NN">POZ</tok> <tok cat="NN">domain</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">function</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
869292404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="S"><cons cat="NP" ref="i47" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">DNA</tok> <tok cat="NN">sequence</tok></cons> <cons cat="VP"><tok cat="VBN">selected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="NP"><tok cat="JJ">recombinant</tok> <tok cat="NN">BCL-6</tok></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i47" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">BCL-6</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NN">B-cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
869292405@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">transfectin</tok> <tok cat="NNS">experiments</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">BCL6</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">repress</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">promoters</tok></cons> <cons cat="VP"><tok cat="VBN">linked</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">DNA</tok> <tok cat="NN">target</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">DNA-binding</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">intact</tok> <tok cat="JJ">N-terminal</tok> <tok cat="NN">half</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
869292406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">part</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">BCL6</tok> <tok cat="NN">molecule</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">autonomous</tok> <tok cat="NN">transrepressor</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">noncontiguous</tok> <tok cat="NNS">regions</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">POZ</tok> <tok cat="NN">motif</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">mediate</tok> <cons cat="NP"><tok cat="JJ">maximum</tok> <tok cat="JJ">transrepressive</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
869292407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">BCL-6</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">function</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">sequence-specific</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">repressor</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">implications</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">BCL6</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="JJ">lymphoid</tok> <tok cat="NN">development</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">lymphomagenesis</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
869329601@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Signals</tok></cons> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="JJR">stronger</tok></cons> <cons cat="PP" ref="i101" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">neonatal</tok> <cons cat="NP" null="QSTN"></cons></cons></cons><cons cat="PP" id="i101"><tok cat="IN">than</tok> <cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
869329602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i48" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">background</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">defects</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immune</tok> <tok cat="NN">reactivity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">neonates</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="ADVP"><tok cat="RB">fully</tok></cons> <cons cat="VP"><tok cat="VBN">elucidated</tok><cons cat="NP" ref="i48" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
869329603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">As</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">central</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">immune</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">inflammatory</tok></cons></cons> <tok cat="NNS">responses</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">authors</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">analysed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">umbilical</tok> <tok cat="NN">cord</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
869329604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i49" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">tested</tok> <cons cat="NP" ref="i49" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><cons cat="VP"><tok cat="VBG">quantitating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">oligonucleotide</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">consensus</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">binding</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
869329605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" null="NONE"></cons></cons></cons><cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">dibutyrate/calcium</tok> <tok cat="NN">ionophore</tok> <tok cat="NN">A23187</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PDBu/iono</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">combination</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">clearly</tok> <tok cat="JJR">higher</tok></cons> <tok cat="JJ">nuclear</tok> <tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">neonatal</tok> <cons cat="NP" null="QSTN"></cons></cons></cons></cons><cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
869329606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i50" role="SBJ"><tok cat="DT">This</tok> <tok cat="JJR">higher</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="JJ">restricted</tok> <cons cat="NP" ref="i50" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">CD4+</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">subset</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
869329607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">antibodies</tok></cons> <cons cat="VP"><tok cat="VBN">directed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">major</tok> <tok cat="NNS">components</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p50</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">RelA</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">p65</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="NNS">proteins</tok><tok cat="COMMA">,</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">indicated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">composition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">similar</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">neonatal</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">adult</tok></cons></cons> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
869329608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i51" role="SBJ"><tok cat="JJ">neonatal</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">exposed</tok> <cons cat="NP" ref="i51" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">oxidative</tok> <tok cat="JJ">stress-inducing</tok></cons> <tok cat="NNS">signals</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">delivery</tok></cons></cons></cons></cons> <tok cat="CC">and/or</tok> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">inherently</tok></cons> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="RBR">more</tok> <tok cat="JJ">sensitive</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">activating</tok> <tok cat="NNS">signals</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
869580001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Characterization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Grb2-binding</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">platelets</tok></cons> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">Fc</tok> <tok cat="NN">gamma</tok> <tok cat="NN">RIIA</tok> <tok cat="NN">cross-linking</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
869580002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i68" role="SBJ"><cons cat="ADJP"><tok cat="NN">Glutathione-S-transferase</tok> <tok cat="LRB">(</tok><tok cat="NN">GST</tok><tok cat="RRB">)</tok><tok cat="NN">-Grb2</tok></cons> <tok cat="NN">fusion</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i68" null="NONE"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">identify</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">potential</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">Grb2-binding</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">platelet</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">platelet</tok> <tok cat="NN">low-affinity</tok> <tok cat="NN">IgG</tok> <tok cat="NN">receptor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Fc</tok> <tok cat="NN">gamma</tok> <tok cat="NN">RIIA</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
869580003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">Two</tok> <tok cat="NN">tyrosine</tok> <tok cat="NNS">phosphoproteins</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="QP"><tok cat="CD">38</tok> <tok cat="CC">and</tok> <tok cat="CD">63</tok></cons> <tok cat="NN">kD</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">SH2</tok> <tok cat="NN">domain</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Grb2</tok></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="VBG">following</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Fc</tok> <tok cat="NN">gamma</tok> <tok cat="NN">RIIA</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">platelets</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
869580004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i69" role="SBJ"><tok cat="DT">Both</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">particulate</tok> <tok cat="NN">fraction</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="VBG">following</tok> <cons cat="NP"><tok cat="NN">platelet</tok> <tok cat="NN">activation</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i69" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">GST-construct</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">SH2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">SH3</tok></cons></cons> <tok cat="NNS">domains</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">phospholipase</tok> <tok cat="NN">C</tok> <tok cat="NN">gamma</tok> <tok cat="CD">1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
869580005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">p38</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><cons cat="ADVP"><tok cat="RB">also</tok></cons> <tok cat="VBZ">forms</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">complex</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok> <tok cat="NN">csk</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">stimulated</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">substrate</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">kinase</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
869580006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">SH3</tok> <tok cat="NNS">domains</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Grb2</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">form</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">stable</tok> <tok cat="NN">complex</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">SOS1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">75</tok> <tok cat="NN">kD</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">120</tok> <tok cat="NN">kD</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i71"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i71" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">undergo</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><cons cat="NP"><tok cat="NN">Fc</tok> <tok cat="NN">gamma</tok> <tok cat="NN">RIIA</tok></cons> <tok cat="JJ">stimulated</tok></cons> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
869580007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i74" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">75-kD</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">recognized</tok> <cons cat="NP" ref="i74" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">antibodies</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">SLP-76</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i75"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i75" id="i76" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" ref="i76" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">sequenced</tok><cons cat="NP" ref="i74" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
869580008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i77" role="SBJ"><cons cat="NP"><tok cat="NN">Tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p38</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p63</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i77" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">platelets</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="NN">tyrosine</tok> <tok cat="JJ">kinase-linked</tok></cons> <tok cat="NN">receptor</tok> <tok cat="NN">agonist</tok> <tok cat="NN">collagen</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="NN">G</tok> <tok cat="JJ">protein-coupled</tok></cons> <tok cat="NN">receptor</tok> <tok cat="NN">agonist</tok> <tok cat="NN">thrombin</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" id="i78" role="SBJ"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SLP-76</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">only</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i78" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">collagen-stimulated</tok> <tok cat="NNS">platelets</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
869580009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i79" role="SBJ"><cons cat="NP"><tok cat="NN">p38</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p63</tok></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">docking</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">Grb2</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" ref="i79" role="SBJ" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">thereby</tok></cons> <cons cat="VP"><tok cat="VBG">linking</tok> <cons cat="NP"><tok cat="NN">Grb2</tok> <tok cat="JJ">SH3-binding</tok> <tok cat="NNS">proteins</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">SOS1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">SLP-76</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p120</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">downstream</tok> <tok cat="NN">signalling</tok> <tok cat="NNS">events</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
869583801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Granulocyte-macrophage</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok></cons> <cons cat="ADVP"><tok cat="RB">preferentially</tok></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">94-kD</tok> <tok cat="NN">STAT5A</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">80-kD</tok> <tok cat="NN">STAT5A</tok> <tok cat="NN">isoform</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
869583802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Granulocyte-macrophage</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GM-CSF</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><tok cat="JJ">immediate</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">Janus</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">JAK2</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">STAT5</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="NN">pathway</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
869583803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Recent</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NNS">homologues</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT5</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">STAT5A</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT5B</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJR">lower</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">weight</tok> <tok cat="NNS">variants</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT5</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
869583804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" ref="i35" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">define</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">STAT5</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">homologues</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJR">lower</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">weight</tok> <tok cat="NN">variant</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <cons cat="VP"><tok cat="VBN">differentiated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">culture</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">macrophage-CSF</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">M-CSF</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i35" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">measured</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="NN">GM-CSF</tok> <tok cat="JJ">induced</tok></cons> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT5A</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">STAT5B</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">any</tok> <tok cat="JJR">lower</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">weight</tok> <tok cat="NN">STAT5</tok> <tok cat="NNS">isoforms</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@49@@oe@16-12-2010
869583805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP"><tok cat="RB">Freshly</tok> <tok cat="VBN">isolated</tok></cons> <tok cat="NNS">monocytes</tok></cons> <cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">94-kD</tok> <tok cat="NN">STAT5A</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">92-kD</tok> <tok cat="NN">STAT5B</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">80-kD</tok> <tok cat="NN">STAT5A</tok> <tok cat="NN">molecule</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
869583806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">Whereas</tok> <cons cat="S"><cons cat="NP" id="i100" role="SBJ"><tok cat="JJ">94-kD</tok> <tok cat="NN">STAT5A</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">clearly</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="NN">tyrosine</tok> <tok cat="JJ">phosphorylated</tok> <cons cat="NP" ref="i100" null="NONE"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">bound</tok> <cons cat="NP" ref="i100" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">enhancer</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">gamma</tok> <tok cat="NN">response</tok> <tok cat="NN">region</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GRR</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Fc</tok> <tok cat="NN">gamma</tok> <tok cat="NN">RI</tok> <tok cat="NN">gene</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">substantially</tok> <tok cat="JJR">less</tok></cons> <tok cat="NN">tyrosine</tok> <tok cat="JJ">phosphorylated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">STAT5B</tok></cons> <cons cat="VP"><tok cat="VBD">bound</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immobilized</tok> <tok cat="NN">GRR</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
869583807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">Macrophages</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">lost</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">express</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">80-kD</tok> <tok cat="NN">STAT5A</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">retained</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="NN">STAT5A</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
869583808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="JJ">STAT5A-STAT5A</tok> <tok cat="NNS">homodimers</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT5A-STAT5B</tok> <tok cat="NNS">heterodimers</tok></cons></cons> <cons cat="VP"><tok cat="VBD">formed</tok> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">GM-CSF</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
869583809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i36" role="SBJ"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT5A</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">predominates</tok> <cons cat="PP"><tok cat="VBN">compared</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">STAT5B</tok></cons></cons></cons> <cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i40"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" ref="i36" id="i39" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">assayed</tok> <cons cat="NP" ref="i39" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">direct</tok> <tok cat="NN">immunoprecipitation</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">evaluation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">bound</tok> <tok cat="NNS">STATs</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">immobilized</tok> <tok cat="NN">GRR</tok></cons></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i40" null="T"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
869583810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Selective</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT5</tok> <tok cat="NNS">homologues</tok></cons></cons></cons> <cons cat="CONJP"><tok cat="IN">in</tok> <tok cat="NN">addition</tok> <tok cat="TO">to</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">generation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJR">lower</tok> <tok cat="JJ">molecular</tok> <tok cat="NNS">isoforms</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">provide</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">specificity</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">control</tok></cons></cons> <cons cat="PP" role="DTV"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cytokines</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">GM-CSF</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
869586301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">LYSP100-associated</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">domains</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">LANDs</tok></cons><tok cat="RRB">)</tok></cons><tok cat="COLON">:</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">description</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">new</tok> <tok cat="NN">class</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">subnuclear</tok> <tok cat="NNS">structures</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">relationship</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">PML</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">bodies</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@16-12-2010
869586302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i37" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">PML</tok> <tok cat="NN">gene</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">fused</tok> <cons cat="NP" ref="i37" id="i38" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RAR</tok> <tok cat="NN">alpha</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">gene</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">t(15;17)</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">promyelocytic</tok> <tok cat="NN">leukemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">APL</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" ref="i38" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">creating</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">PML-RAR</tok> <tok cat="NN">alpha</tok> <tok cat="NN">fusion</tok> <tok cat="NN">oncoprotein</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
869586303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i39" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">PML</tok> <tok cat="NN">gene</tok> <tok cat="NN">product</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="JJ">localized</tok> <cons cat="NP" ref="i39" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">subnuclear</tok> <tok cat="JJ">dot-like</tok> <tok cat="NNS">structures</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">variously</tok></cons> <tok cat="VBN">termed</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" syn="COOD" role="PRD"><cons cat="NP"><tok cat="NNS">PODs</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNS">ND10s</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Kr</tok> <tok cat="NNS">bodies</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PML</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">bodies</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PML</tok> <tok cat="NNS">NBs</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
869586304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBZ">describes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cloning</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">lymphoid-restricted</tok> <tok cat="NN">gene</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">LYSP100</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i40"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i40" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">homologous</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">another</tok> <tok cat="NN">protein</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i41"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i41" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">localizes</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PML</tok> <tok cat="NNS">NBs</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">SP100</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
869586305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <tok cat="NN">addition</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">SP100</tok> <tok cat="NN">homology</tok> <tok cat="NNS">regions</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="CD">one</tok> <tok cat="NN">LYSP100</tok> <tok cat="NN">cDNA</tok> <tok cat="NN">isoform</tok></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">bromodomain</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">PHD/TTC</tok> <tok cat="NN">domain</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i42"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i42" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">variety</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
869586306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="NP"><tok cat="NN">immunofluorescence</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i43" role="SBJ"><tok cat="NN">LYSP100</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="JJ">localized</tok> <cons cat="NP" ref="i43" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">dots</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i44"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i44" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">surprisingly</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="RB">largely</tok> <tok cat="JJ">nonoverlapping</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">PML</tok> <tok cat="NNS">NBs</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
869586307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">minority</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">LYSP100</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">dots</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">exactly</tok></cons> <cons cat="VP"><tok cat="VBD">colocalized</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PML</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">SP100</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
869586308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">term</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">LYSP100</tok> <tok cat="NNS">structures</tok></cons> <tok cat="LQT">"</tok><cons cat="NP" role="PRD"><tok cat="NNS">LANDs</tok></cons></cons><tok cat="COMMA">,</tok><tok cat="RQT">"</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">LYSP100-associated</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">domains</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
869586309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" id="i45" role="SBJ"><tok cat="NN">LYSP100</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i45" null="NONE"></cons><cons cat="PP"><tok cat="RB">only</tok> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">lymphoid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i46" role="SBJ"><tok cat="NNS">LANDs</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">visualized</tok> <cons cat="NP" ref="i46" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">HeLa</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">transfection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">LYSP100</tok> <tok cat="NN">cDNA</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
869586310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Immunoelectron</tok> <tok cat="NN">microscopy</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">LANDs</tok></cons> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">globular</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">electron-dense</tok></cons></cons> <tok cat="NNS">structures</tok></cons> <cons cat="ADJP"><tok cat="RB">morphologically</tok> <tok cat="JJ">distinct</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">annular</tok> <tok cat="NNS">structures</tok></cons> <cons cat="ADJP"><tok cat="JJ">characteristic</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PML</tok> <tok cat="NNS">NBs</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
869586311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i48" role="SBJ"><tok cat="NNS">LANDs</tok></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP" adv="TMP"><tok cat="RBS">most</tok> <tok cat="RB">often</tok></cons> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" ref="i48" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleoplasm</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" ref="i48" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">membrane</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytoplasm</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">structures</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VBP">traffic</tok> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytoplasm</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
869586312@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">double-immunogold</tok> <tok cat="NN">labeling</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PML</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">LYSP100</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i51" role="SBJ"><tok cat="DT">some</tok> <tok cat="NNS">LANDs</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i51" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">contain</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">PML</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">LYSP100</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
869586313@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i52" role="SBJ"><tok cat="NN">PML</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="JJ">localized</tok> <cons cat="NP" ref="i52" null="NONE"></cons><cons cat="NP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">second</tok> <tok cat="JJ">subnuclear</tok> <tok cat="NN">domain</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i53"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i53" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><cons cat="ADVP" syn="COOD"><cons cat="ADVP"><tok cat="RB">morphologically</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">biochemically</tok></cons></cons> <tok cat="JJ">distinct</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">PML</tok> <tok cat="NNS">NBs</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
869911801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Constitutive</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">interferon</tok> <tok cat="NNS">isotypes</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">leukocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NNS">individuals</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">promonocytic</tok> <tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
869911802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i80" role="SBJ"><cons cat="NP"><tok cat="JJ">Constitutive</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IFN-alpha5</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IFN-beta</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i80" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="JJ">lymphoid</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NNS">individuals</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="VBG">following</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">amplification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IFN</tok> <tok cat="NN">mRNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">reverse</tok> <tok cat="NN">transcriptase-polymerase</tok> <tok cat="NN">chain</tok> <tok cat="NN">reaction</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">direct</tok> <tok cat="NN">sequencing</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">amplified</tok> <tok cat="NN">product</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
869911803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i81" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">activated</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">interferon-induced</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">complex</tok> <tok cat="NN">ISGF3</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i81" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">uninduced</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
869911804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i82" role="SBJ"><cons cat="NP"><tok cat="NN">Culture</tok> <tok cat="NNS">supernatants</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">uninduced</tok> <tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i82" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">ISRE</tok></cons> <cons cat="VP"><tok cat="VBN">cloned</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">upstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">luciferase</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">endogenous</tok> <tok cat="NN">IFN</tok> <tok cat="NN">activity</tok></cons> <cons cat="ADJP"><tok cat="JJ">equivalent</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="QP"><tok cat="RB">approximately</tok> <tok cat="CD">0.3</tok> <tok cat="TO">to</tok> <tok cat="CD">0.5</tok></cons> <tok cat="NN">IU/mL</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
869911805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i84" role="SBJ"><tok cat="DT">This</tok> <tok cat="JJ">endogenous</tok> <tok cat="NN">IFN</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i84" id="i83" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" ref="i83" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">maintaining</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">basal</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">major</tok> <tok cat="NN">histocompatibility</tok> <tok cat="NN">class</tok> <tok cat="CD">I</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">lymphoid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
869911806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i88" role="SBJ"><cons cat="NP"><tok cat="NN">IFN-alpha5</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IFN-beta</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" ref="i88" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="JJ">low</tok> <tok cat="NNS">levels</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NNS">tissues</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">function</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">maintenance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">homeostasis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
870022801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Reversal</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">leukaemia-associated</tok> <tok cat="NN">E2A-HLF</tok> <tok cat="JJ">chimaeric</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
870022802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">E2A-HLF</tok> <tok cat="LRB">(</tok><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">hepatic</tok> <tok cat="NN">leukaemia</tok> <tok cat="NN">factor</tok></cons></cons><tok cat="RRB">)</tok></cons> <tok cat="NN">fusion</tok> <tok cat="NN">gene</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">formed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">action</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">t(17;19)</tok> <tok cat="NN">(q22;p13)</tok> <tok cat="JJ">chromosomal</tok> <tok cat="NN">translocation</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">drives</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">leukaemic</tok> <tok cat="NN">transformation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">B-cell</tok> <tok cat="NNS">precursors</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">remains</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unknown</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
870022803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i37" role="SBJ"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">leukaemia</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">carrying</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">translocation</tok> <tok cat="NN">t(17;19)</tok></cons></cons></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok></cons> <cons cat="VP"><tok cat="VBD">died</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons> <cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i38"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" ref="i37" id="i39" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">programmed</tok> <cons cat="S"><cons cat="NP" ref="i39" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">express</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">dominant-negative</tok> <tok cat="NN">suppressor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok> <tok cat="NN">E2A-HLF</tok></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i38" null="T"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">chimaeric</tok> <tok cat="NN">oncoprotein</tok></cons> <cons cat="ADVP"><tok cat="RB">probably</tok></cons> <cons cat="VP"><tok cat="VBZ">affects</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">survival</tok></cons> <cons cat="CONJP"><tok cat="RB">rather</tok> <tok cat="IN">than</tok></cons> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">growth</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
870022804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i42"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" ref="i40" id="i41" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">introduced</tok> <cons cat="NP" ref="i41" null="NONE"></cons><cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="JJ">pro-B</tok> <tok cat="NNS">lymphocytes</tok></cons></cons><cons cat="ADVP" ref="i42" null="T"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i40" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">oncogenic</tok> <tok cat="NN">E2A-HLF</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBD">reversed</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADJP"><tok cat="JJ">interleukin-3-dependent</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">p53-mediated</tok></cons></cons> <tok cat="NN">apoptosis</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
870022805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">close</tok> <tok cat="NN">homology</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">basic</tok> <tok cat="NN">region/leucine</tok> <tok cat="NN">zipper</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">bZIP</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="UCP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">DNA-binding</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">dimerization</tok></cons></cons> <tok cat="NN">domain</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HLF</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CES-2</tok> <tok cat="NN">cell-death</tok> <tok cat="NN">specification</tok> <tok cat="NN">protein</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Caenorhabditis</tok> <tok cat="NNS">elegans</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">model</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">leukaemogenesis</tok></cons></cons> <cons cat="SBAR"><cons cat="WHPP" id="i43"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">E2A-HLF</tok></cons> <cons cat="VP"><tok cat="VBZ">blocks</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">early</tok> <tok cat="NN">step</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP"><tok cat="RB">evolutionarily</tok> <tok cat="VBN">conserved</tok></cons> <tok cat="NN">cell-death</tok> <tok cat="NN">pathway</tok></cons></cons></cons><cons cat="PP" ref="i43" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@16-12-2010
870246601@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i42" role="SBJ"><tok cat="JJ">Protein-tyrosine</tok> <tok cat="NN">kinase</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i42" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">lipopolysaccharide</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="NN">1beta</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NFkappaB</tok> <tok cat="NN">activation</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">NFkappaB</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">translocation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
870246602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i43" role="SBJ"><cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="NN">1beta</tok> <tok cat="NN">protein</tok> <tok cat="NN">production</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">steady</tok> <tok cat="NN">state</tok> <tok cat="NN">mRNA</tok> <tok cat="NNS">levels</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">increased</tok> <cons cat="NP" ref="i43" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">lipopolysaccharide</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">predominantly</tok></cons> <tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">result</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">interleukin</tok> <tok cat="NN">1beta</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
870246603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i44" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="NN">1beta</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">cytokines</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="CD">6</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">alpha</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i44" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">NFkappaB</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
870246604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">Since</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">recent</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i45" role="SBJ"><tok cat="JJ">lipopolysaccharide-induced</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i45" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">NFkappaB</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">translocation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i46" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">sought</tok> <cons cat="S"><cons cat="NP" ref="i46" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" id="i47" role="SBJ"><cons cat="NP"><tok cat="NN">NFkappaB</tok></cons> <cons cat="VP"><tok cat="VBN">translocated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">absence</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">active</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" ref="i47" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">stimulating</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
870246605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="JJ">pro-monocytic</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">THP-1</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">lipopolysaccharide-induced</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="NN">1beta</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
870246606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i48" role="SBJ"><tok cat="NN">Tyrosine</tok> <tok cat="NNS">kinases</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i48" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">lipopolysaccharide-mediated</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFkappaB</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
870246607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">absence</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok> <tok cat="NN">activity</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok> <cons cat="S" ref="i50" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFkappaB</tok></cons></cons> <cons cat="S" id="i50"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">stimulate</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">impaired</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
870246608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">This</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">specific</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">NFkappaB</tok></cons></cons></cons></cons><tok cat="COLON">;</tok></cons> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">absence</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok> <tok cat="NN">activity</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i51" role="SBJ"><tok cat="JJ">AP-1-dependent</tok> <tok cat="NN">transcription</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">enhanced</tok><cons cat="NP" ref="i51" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@22@@oe@16-12-2010
870246609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="SBAR"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">lipopolysaccharide-induced</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NNS">mediators</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">obligatory</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">lipopolysaccharide</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
870283801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Elevated</tok> <tok cat="JJ">cyclic</tok> <tok cat="NN">AMP</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">NF-kappaB-mediated</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
870283802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">NF-kappaB/Rel</tok> <tok cat="NN">family</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">regulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inducible</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">many</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="JJ">human</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
870283803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i22"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">agents</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i23"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i23" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">elevate</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">cAMP</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNFalpha</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">tissue</tok> <tok cat="NN">factor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NN">leukocyte</tok> <tok cat="NN">adhesion</tok> <tok cat="NN">molecule-1</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">vascular</tok> <tok cat="NN">cell</tok> <tok cat="NN">adhesion</tok> <tok cat="NN">molecule-1</tok></cons></cons> <tok cat="NNS">genes</tok></cons></cons></cons><cons cat="PP" ref="i22" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
870283804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="CC">Both</tok> <cons cat="NP"><tok cat="NN">forskolin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">dibutyryl</tok> <tok cat="NN">cAMP</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i24"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i24" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">elevate</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">cAMP</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">independent</tok> <tok cat="NNS">mechanisms</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">TNFalpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">tissue</tok> <tok cat="NN">factor</tok></cons></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
870283805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i25" role="SBJ"><cons cat="NP"><tok cat="NN">Induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">NF-kappaB-dependent</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">transiently</tok> <tok cat="VBN">transfected</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">monocytic</tok> <tok cat="NN">THP-1</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">umbilical</tok> <tok cat="NN">vein</tok> <tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i25" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">elevated</tok> <tok cat="NN">cAMP</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">overexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">catalytic</tok> <tok cat="NN">subunit</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">A</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PKA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
870283806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Elevated</tok> <tok cat="NN">cAMP</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">prevent</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p50/p65</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Rel/p65</tok></cons></cons> <tok cat="NNS">heterodimers</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VB">decrease</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <tok cat="VB">modify</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">TNFalpha-induced</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
870283807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Functional</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i26" role="SBJ"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">plasmid</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="JJ">multimerized</tok> <tok cat="NN">kappaB</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i26" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">agents</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i27"><tok cat="IN">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i27" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">elevate</tok> <cons cat="NP"><tok cat="NN">cAMP</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">overexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">catalytic</tok> <tok cat="NN">subunit</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PKA</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
870283808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBZ">indicates</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i28" role="SBJ"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PKA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">reduces</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">distinct</tok> <tok cat="NN">set</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">genes</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i28" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inhibiting</tok> <cons cat="NP"><tok cat="JJ">NF-kappaB-mediated</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
870284901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Characterization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">new</tok> <tok cat="NN">isoform</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NFAT</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">gene</tok> <tok cat="NN">family</tok> <tok cat="NN">member</tok></cons></cons></cons> <tok cat="NN">NFATc</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="VBN">published</tok> <tok cat="NN">erratum</tok></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNP">J</tok> <tok cat="NNP">Biol</tok> <tok cat="NNP">Chem</tok></cons> <cons cat="NP" adv="TMP"><tok cat="CD">1996</tok> <tok cat="NNP">Dec</tok> <tok cat="CD">27</tok></cons><tok cat="COLON">;</tok><cons cat="NP"><tok cat="CD">271</tok><tok cat="LRB">(</tok><tok cat="CD">52</tok><tok cat="RRB">)</tok></cons><tok cat="COLON">:</tok><cons cat="NP"><tok cat="CD">33705</tok></cons></cons></cons></cons></cons><tok cat="RRB">]</tok></cons></cons>@@@@1@39@@oe@16-12-2010
870284902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="ADJP"><tok cat="NN">cyclosporin</tok> <tok cat="NN">A</tok> <tok cat="LRB">(</tok><tok cat="NN">CsA</tok><tok cat="RRB">)</tok><tok cat="JJ">/FK506-sensitive</tok></cons> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NFAT</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">key</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inducible</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
870284903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" id="i51" role="SBJ"><tok cat="NN">NFAT</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i51" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">inducible</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">several</tok> <cons cat="ADJP"><tok cat="JJ">non-T</tok> <tok cat="JJ">immune</tok></cons> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i50" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NFAT</tok> <tok cat="NN">gene</tok> <tok cat="NN">family</tok> <tok cat="NNS">members</tok></cons> <cons cat="VP"><tok cat="VBN">characterized</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">date</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" ref="i50" null="NONE"></cons><cons cat="PP"><tok cat="RB">only</tok> <tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
870284904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" ref="i52" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="TO">To</tok> <cons cat="ADVP"><tok cat="RBR">further</tok></cons> <cons cat="VP"><tok cat="VB">characterize</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NFAT</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">demonstrate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">gene</tok> <tok cat="NN">specificity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFAT</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i52" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">isolation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">characterization</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cDNA</tok> <tok cat="NN">clone</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Raji</tok> <tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
870284905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">cDNA</tok> <tok cat="NN">clone</tok></cons> <cons cat="VP"><tok cat="VBZ">encodes</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">new</tok> <tok cat="NN">isoform</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">NFATc.beta</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NFAT</tok> <tok cat="NN">gene</tok> <tok cat="NN">family</tok> <tok cat="NN">member</tok> <tok cat="NN">NFATc</tok></cons> <tok cat="LRB">(</tok><cons cat="VP"><tok cat="VBN">designated</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NN">NFATc.alpha</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
870284906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">amino</tok> <tok cat="NN">acid</tok> <tok cat="NN">sequence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFATc.beta</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">differs</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFATc.alpha</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="JJ">NH2-terminal</tok> <tok cat="CD">29</tok> <tok cat="NNS">residues</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">additional</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">142</tok> <tok cat="NNS">residues</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">COOH</tok> <tok cat="NN">terminus</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
870284907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Northern</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">a</tok> <tok cat="NN">probe</tok></cons> <cons cat="VP"><tok cat="VBG">encompassing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">common</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">isoforms</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NN">mRNA</tok> <tok cat="NNS">species</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><cons cat="QP"><tok cat="CD">2.7</tok> <tok cat="CC">and</tok> <tok cat="CD">4.5</tok></cons> <tok cat="NN">kilobase</tok></cons> <tok cat="NNS">pairs</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">an</tok> <tok cat="JJ">NFATc.beta-specific</tok> <tok cat="NN">probe</tok></cons> <cons cat="VP"><tok cat="VBD">detected</tok> <cons cat="NP"><cons cat="NP"><tok cat="RB">only</tok> <tok cat="DT">the</tok> <tok cat="NN">4.5-kilobase</tok> <tok cat="NN">pair</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i53"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i53" id="i54" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">preferentially</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i54" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">spleen</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
870284908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i55" role="SBJ"><cons cat="NP"><tok cat="JJ">Transient</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFATc.beta</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S"><cons cat="NP" ref="i55" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">activating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">interleukin-2</tok> <tok cat="JJ">NFAT-driven</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">stimulated</tok> <tok cat="NN">Jurkat</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">CsA-sensitive</tok> <tok cat="NN">manner</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
870284909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">NFATc.beta</tok></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><tok cat="CC">neither</tok> <cons cat="VP"><tok cat="VBD">bound</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">kappa3</tok> <tok cat="NN">element</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">NFAT-binding</tok> <tok cat="NN">site</tok></cons> <tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor-alpha</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons> <tok cat="CC">nor</tok> <cons cat="VP"><tok cat="VBD">activated</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor-alpha</tok> <tok cat="NN">promoter</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">cotransfection</tok> <tok cat="NN">assays</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
870284910@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">different</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">members</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NNS">isoforms</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFAT</tok> <tok cat="NN">gene</tok> <tok cat="NN">family</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">inducible</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NN">cytokine</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
870416501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Retinoic</tok> <tok cat="NN">acid</tok></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interferon</tok> <tok cat="JJ">regulatory</tok> <tok cat="JJ">factor-1</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
870416502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">All-trans-retinoic</tok> <tok cat="NN">acid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ATRA</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">drug</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">choice</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">promyelocytic</tok> <tok cat="NN">leukemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">APL</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
870416503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">ATRA</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">APL</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="NNS">granulocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
870416504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i63" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">ATRA-dependent</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">growth</tok> <tok cat="NN">inhibition</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <tok cat="RB">not</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">presently</tok></cons> <cons cat="VP"><tok cat="VBN">understood</tok><cons cat="NP" ref="i63" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
870416505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i64" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">NB4</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i65"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i65" id="i66" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" ref="i66" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">bone</tok> <tok cat="NN">marrow</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">patient</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">APL</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">relapse</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i64" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">model</tok> <tok cat="NN">system</tok></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i106" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">study</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">growth</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">APL</tok> <tok cat="NNS">cells</tok></cons></cons></cons><cons cat="ADVP" ref="i106" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
870416506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">Because</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interferon</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IFN</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="JJ">regulatory</tok> <tok cat="NNS">factors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IRF-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IRF-2</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="JJ">IFN-inducible</tok> <tok cat="NN">gene</tok> <tok cat="NNS">products</tok></cons></cons> <cons cat="VP"><tok cat="VBP">regulate</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">growth</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">analyzed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ATRA</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
870416507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">ATRA</tok></cons> <cons cat="ADVP"><tok cat="RB">directly</tok></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="NN">IRF-1</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i68" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">followed</tok> <cons cat="NP" ref="i68" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IRF-2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">2'-5'</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">oligoadenylate</tok> <tok cat="NN">synthetase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">OAS</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJR">slower</tok> <tok cat="NNS">kinetics</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
870416508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <tok cat="NN">addition</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">NB4</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">ATRA</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">activated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IRF-1</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HL-60</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">U937</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">THP-1</tok></cons></cons> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i69"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" ref="i69" null="T"></cons><cons cat="NP"><tok cat="DT">all</tok></cons></cons> <cons cat="VP"><tok cat="VBP">respond</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">ATRA</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">growth</tok> <tok cat="NN">inhibition</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
870416509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i70" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <cons cat="ADJP"><cons cat="ADJP"><tok cat="JJR">more</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="ADJP"><tok cat="JJ">additive</tok></cons></cons></cons> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">IRF-1</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">seen</tok> <cons cat="NP" ref="i70" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ATRA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">NB4</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
870416510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">ATRA</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">transducer</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">activator</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">STAT</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">activation</tok> <tok cat="NNS">pathways</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i72" role="SBJ"><tok cat="DT">an</tok> <tok cat="JJ">alternate</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i72" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">IRF-1</tok> <tok cat="NN">gene</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
870416511@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">ATRA-induced</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IRF-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">activator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">repressor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transformation</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" syn="COOD" role="PRD"><cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">ATRA-induced</tok> <tok cat="NN">growth</tok> <tok cat="NN">inhibition</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">basis</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">synergistic</tok> <tok cat="NNS">actions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ATRA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IFNs</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NN">leukemia</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
870744501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Cytokine-modulating</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tepoxalin</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">new</tok></cons> <cons cat="ADJP"><tok cat="JJ">potential</tok></cons></cons> <tok cat="NN">antirheumatic</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@10@@oe@16-12-2010
870744502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Tepoxalin</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">new</tok> <tok cat="JJ">dual</tok> <tok cat="NN">cyclooxygenase/5-lipoxygenase</tok> <tok cat="JJ">anti-inflammatory</tok> <tok cat="NN">compound</tok></cons> <cons cat="PP"><cons cat="ADVP" adv="TMP"><tok cat="RB">currently</tok></cons> <tok cat="IN">under</tok> <cons cat="NP"><tok cat="JJ">clinical</tok> <tok cat="NN">investigation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
870744503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">possess</tok> <cons cat="NP"><tok cat="JJ">anti-inflammatory</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">variety</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">animal</tok> <tok cat="NNS">models</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RBR">more</tok> <tok cat="RB">recently</tok></cons> <tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="JJ">induced</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
870744504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">current</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">conducted</tok> <cons cat="NP" ref="i2" null="NONE"></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">evaluate</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">cytokine</tok></cons> <tok cat="NN">modulating</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">tepoxalin</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">iron</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">effects</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
870744505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PBMC</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">OKT3/PMA</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">tepoxalin</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">lymphocyte</tok></cons> <tok cat="NN">proliferation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">IC50</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">6</tok> <tok cat="NN">microM</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
870744506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Additionally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">LTB4</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">IC50</tok></cons> <cons cat="ADJP"><tok cat="JJ">=</tok> <cons cat="NP"><tok cat="CD">0.5</tok> <tok cat="NN">microM</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-6</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">TNF</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">IC50</tok></cons> <cons cat="ADJP"><tok cat="JJ">=</tok> <cons cat="NP"><cons cat="QP"><tok cat="CD">10-12</tok></cons> <tok cat="NN">microM</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
870744507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="NN">Cytotoxicity</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">concentrations</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@16-12-2010
870744508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="JJ">Add-back</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cytokines</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">IL-6</tok></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">LTB4</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">conditioned</tok> <tok cat="NNS">media</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">failed</tok> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">restore</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">proliferative</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">tepoxalin</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
870744509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">concurrent</tok> <tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">iron</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">ferrous</tok> <cons cat="NP" ref="i5" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">ferric</tok> <cons cat="NP" ref="i5" null="RNR"></cons></cons><cons cat="NP" id="i5"><tok cat="NN">chloride</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NN">iron</tok> <tok cat="NNS">salts</tok></cons></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">reversed</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">proliferation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">caused</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">tepoxalin</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
870744510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Tepoxalin</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">acts</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">several</tok> <cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
870744511@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="NN">Tepoxalin</tok><tok cat="POS">'s</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">reversed</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">iron</tok> <tok cat="NNS">salts</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
870744512@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">action</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">tepoxalin</tok></cons></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">proliferation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">PBMC</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">part</tok></cons></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">due</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">ability</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">reduce</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">amount</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">available</tok> <tok cat="NN">iron</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="VBN">decreased</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">subsequent</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok></cons> <tok cat="NN">production</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
870919101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">oriP</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">EBNA</tok> <tok cat="NN">gene</tok> <tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="JJ">virus-immortalized</tok></cons> <tok cat="JJ">lymphoblastoid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
870919102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">During</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok> <tok cat="JJ">latent</tok> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i31" role="SBJ"><cons cat="NP"><tok cat="CD">six</tok> <tok cat="JJ">viral</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">antigens</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EBNAs</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i31" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">promoters</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Cp</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">Wp</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i32"><tok cat="WPP">whose</tok> <tok cat="NNS">activities</tok></cons> <cons cat="S"><cons cat="NP" ref="i32" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">mutually</tok> <tok cat="JJ">exclusive</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
870919103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">Upon</tok> <cons cat="NP"><tok cat="NN">infection</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i33" role="SBJ"><tok cat="NN">Wp</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">initially</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">active</tok></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" ref="i33" id="i34" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">followed</tok> <cons cat="NP" ref="i34" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">switch</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">Cp</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">duration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">latency</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
870919104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i35" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">region</tok></cons> <cons cat="ADVP"><tok cat="JJ">upstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Cp</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP" ref="i35" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cis</tok> <tok cat="NNS">elements</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" id="i104" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" ref="i104" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">activities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">EBNA</tok> <tok cat="NN">gene</tok> <tok cat="NNS">promoters</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">established</tok> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="VBN">immortalized</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">lymphoblastoid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">LCLs</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
870919105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">determined</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">oriP</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">origin</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">episomal</tok> <tok cat="NN">maintenance</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">latency</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">efficient</tok> <tok cat="NN">transcription</tok> <tok cat="NN">initiation</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="NN">Cp</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">Wp</tok></cons></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">LCLs</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">some</tok> <cons cat="NP"><tok cat="NN">Burkitt</tok><tok cat="POS">'s</tok></cons> <tok cat="NN">lymphoma</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
870919106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Deletion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">EBNA2-dependent</tok> <tok cat="NN">enhancer</tok></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="ADVP"><tok cat="RB">upstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Cp</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="QP"><tok cat="FW">ca.</tok> <tok cat="CD">two-</tok> <tok cat="TO">to</tok> <tok cat="JJ">fivefold</tok></cons> <tok cat="NN">reduction</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Cp</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">LCLs</tok></cons> <cons cat="VP"><tok cat="VBN">assayed</tok><cons cat="NP" null="NONE"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
870919107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="RBR">More</tok> <tok cat="JJ">extensive</tok></cons> <tok cat="NN">deletion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">sequences</tok></cons> <cons cat="ADVP"><tok cat="JJ">upstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Cp</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">EBNA2-dependent</tok> <tok cat="NN">enhancer</tok></cons></cons><tok cat="COMMA">,</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">nearly</tok> <tok cat="JJ">complete</tok></cons> <tok cat="NN">loss</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Cp</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
870919108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i36" role="SBJ"><cons cat="NP"><tok cat="DT">This</tok> <tok cat="NN">loss</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">activity</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i36" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">correlate</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">deletion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NN">CCAAT</tok> <tok cat="NNS">boxes</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">proximal</tok> <tok cat="NN">CCAAT</tok> <tok cat="NN">box</tok></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="NN">bp</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-61</tok></cons> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="CD">-65</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">distal</tok> <tok cat="NN">CCAAT</tok> <tok cat="NN">box</tok></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="UCP" syn="COOD"><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="NN">bp</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-253</tok></cons> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="CD">-257</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">upstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Cp</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
870919109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Site-directed</tok> <tok cat="NN">mutagenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NN">cis</tok> <tok cat="NNS">elements</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Cp</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">highly</tok> <tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">properly</tok> <tok cat="VBN">positioned</tok></cons> <tok cat="NN">CCAAT</tok> <tok cat="NN">box</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">dependence</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">distal</tok> <tok cat="NN">CCAAT</tok> <tok cat="NN">box</tok></cons> <cons cat="ADJP"><tok cat="JJ">apparent</tok> <cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i37"><tok cat="RB">only</tok> <tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i38" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">proximal</tok> <tok cat="NN">CCAAT</tok> <tok cat="NN">box</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">deleted</tok> <cons cat="NP" ref="i38" null="NONE"></cons></cons><tok cat="CC">or</tok> <cons cat="VP"><tok cat="VBN">mutated</tok><cons cat="NP" ref="i38" null="NONE"></cons></cons></cons><cons cat="ADVP" ref="i37" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
870919110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Deletion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">glucocorticoid</tok> <tok cat="NN">response</tok> <tok cat="NNS">elements</tok></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="ADVP"><cons cat="NP"><tok cat="FW">ca.</tok> <tok cat="NN">bp</tok> <tok cat="CD">-850</tok></cons> <tok cat="RB">upstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Cp</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">result</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">significant</tok> <tok cat="NN">loss</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
870919111@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">general</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">deletions</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i40"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i40" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">diminished</tok> <cons cat="NP"><tok cat="JJ">Cp</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">Wp</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">suppression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">Wp</tok> <tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">interference</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">Cp</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
870919112@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">identification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">oriP</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">EBNA2-dependent</tok> <tok cat="NN">enhancer</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">major</tok> <tok cat="JJ">positive</tok> <tok cat="NN">cis</tok> <tok cat="NNS">elements</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" id="i105" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" ref="i105" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Cp</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">LCL</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i41" role="SBJ"><tok cat="NN">EBNA</tok> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">largely</tok></cons> <cons cat="VP"><tok cat="VBN">autoregulated</tok> <cons cat="NP" ref="i41" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">EBNA</tok> <tok cat="CD">1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">EBNA</tok> <tok cat="CD">2</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
870920901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Various</tok> <tok cat="NNS">modes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">basic</tok> <tok cat="JJ">helix-loop-helix</tok> <tok cat="JJ">protein-mediated</tok></cons> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">lymphoid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
870920902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="ADJP"><tok cat="RB">transcriptionally</tok> <tok cat="JJ">regulatory</tok></cons> <tok cat="NNS">regions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">lymphomagenic</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Akv</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">SL3-3</tok></cons></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">leukemia</tok> <tok cat="NNS">retroviruses</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">MLVs</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">contain</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">types</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">E-box</tok> <tok cat="NN">consensus</tok> <tok cat="NNS">motifs</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CAGATG</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
870920903@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">One</tok> <tok cat="NN">type</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">EA/S</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">upstream</tok> <tok cat="NN">promoter</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">other</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">E(gre)</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">tandem</tok> <tok cat="NN">repeat</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">enhancer</tok> <tok cat="NNS">properties</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
870920904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">requirements</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">individual</tok> <tok cat="NNS">E-boxes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">MLV</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">regulation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
870920905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">lymphoid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="ADVP"><tok cat="RB">only</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">E(gre)-binding</tok> <tok cat="NN">protein</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="VP"><tok cat="VBD">included</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ALF1</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">HEB</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">E2A</tok></cons></cons> <tok cat="JJ">basic</tok> <tok cat="JJ">helix-loop-helix</tok> <tok cat="NNS">proteins</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
870920906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i35" role="SBJ"><tok cat="JJ">Ectopic</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ALF1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">E2A</tok></cons></cons> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBD">required</tok> <cons cat="NP"><tok cat="JJ">intact</tok> <tok cat="NN">E(gre)</tok> <tok cat="NNS">motifs</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S"><cons cat="NP" ref="i35" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">mediating</tok> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
870920907@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNP">ALF1</tok></cons> <cons cat="VP"><tok cat="VBD">transactivated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Akv</tok> <tok cat="NN">MLV</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="NN">E(gre)</tok> <tok cat="NNS">motifs</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">equally</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">E2A</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBD">required</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">promoter-proximal</tok> <tok cat="NN">E(gre)</tok> <tok cat="NN">motif</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
870920908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">T-</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">B-cell</tok></cons></cons> <tok cat="NNS">lines</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">E(gre)</tok> <tok cat="NNS">motifs</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="PP" role="PRD"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">major</tok> <tok cat="NN">importance</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">Akv</tok> <tok cat="NN">MLV</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">EA/S</tok> <tok cat="NN">motif</tok></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="DT">some</tok> <tok cat="NN">effect</tok></cons><cons cat="PP" null="QSTN"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
870920909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="CC">neither</tok> <cons cat="NP"><tok cat="NN">E(gre)</tok></cons> <tok cat="CC">nor</tok> <cons cat="NP"><tok cat="NN">EA/S</tok></cons></cons> <tok cat="NNS">motifs</tok></cons> <cons cat="VP"><tok cat="VBD">contributed</tok> <cons cat="ADVP"><tok cat="RB">pronouncedly</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Akv</tok> <tok cat="NN">MLV</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NIH</tok> <tok cat="NN">3T3</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">lacking</tok> <cons cat="NP"><tok cat="JJ">DNA-binding</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ALF1</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">HEB</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">E2A</tok></cons></cons> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
870920910@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i36" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">Id1</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i36" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">repress</tok> <cons cat="NP"><tok cat="NN">ALF1</tok> <tok cat="NN">activity</tok></cons> <cons cat="ADVP" syn="COOD"><cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
870920911@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="JJ">ectopic</tok> <tok cat="NN">Id1</tok></cons> <cons cat="VP"><tok cat="VBD">repressed</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">E(gre)-directed</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="ADJP"><tok cat="JJ">EA/S-directed</tok></cons></cons> <tok cat="NN">MLV</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">lymphoid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
870920912@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">conclusion</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">E(gre)</tok> <tok cat="NNS">motifs</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="VBG">interacting</tok> <tok cat="JJ">basic</tok> <tok cat="JJ">helix-loop-helix</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">important</tok> <tok cat="NNS">determinants</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">MLV</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">lymphocytic</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
870927801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok> <tok cat="JJ">posttranscriptional</tok> <tok cat="NN">trans-activator</tok> <tok cat="NN">Rex</tok></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">export</tok> <tok cat="NN">signal</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
870927802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i31" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">Rex</tok> <tok cat="NN">protein</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i31" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">export</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">unspliced</tok> <tok cat="JJ">viral</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons> <tok cat="CC">and</tok><tok cat="COMMA">,</tok> <tok cat="RB">therefore</tok><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">virus</tok> <tok cat="NN">replication</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
870927803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">manuscript</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Rex</tok></cons> <cons cat="VP"><tok cat="VBZ">shuttles</tok> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytoplasm</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">its</tok> <tok cat="NN">activation</tok> <tok cat="NN">domain</tok></cons> <cons cat="VP"><tok cat="VBZ">constitutes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">export</tok> <tok cat="NN">signal</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i32"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i32" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">specifies</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">efficient</tok> <tok cat="NN">transport</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytoplasm</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
870927804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">model</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">Rex-mediated</tok> <tok cat="NN">trans-activation</tok></cons></cons> <cons cat="SBAR"><cons cat="WHPP" id="i33"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i34" role="SBJ"><tok cat="JJ">Rex-viral</tok> <tok cat="NN">mRNA</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">targeted</tok> <cons cat="NP" ref="i34" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">export</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">direct</tok> <tok cat="NN">action</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons></cons><cons cat="PP" ref="i33" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
870963601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">Effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-10</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">LPS-induced</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">AP-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">NF-IL6</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i49"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i49" id="i50" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i50" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">IL-6</tok> <tok cat="NN">regulation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
870963602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i51" role="SBJ"><cons cat="NP"><tok cat="NN">Interleukin-10</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-10</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP" tpc="TPC" id="i102"><tok cat="IN">like</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" ref="i51" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons><cons cat="PP" ref="i102" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
870963603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i52" role="SBJ"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons> <cons cat="VP"><tok cat="VBP">inhibit</tok> <cons cat="NP"><tok cat="JJ">LPS-induced</tok> <tok cat="NN">IL-6</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok></cons></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i52" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inhibiting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">rate</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-6</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
870963604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">strong</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-6</tok> <tok cat="NN">transcription</tok> <tok cat="NN">rate</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">prompted</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">us</tok></cons> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">study</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-10</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
870963605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">questioned</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <tok cat="CC">or</tok> <tok cat="RB">not</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">IL-10</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons> <cons cat="VP"><tok cat="VBD">affected</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i53"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i53" id="i54" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" ref="i54" id="i55" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i55" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-6</tok> <tok cat="NN">transcription</tok> <tok cat="NN">rate</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="RB">namely</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">activator</tok> <tok cat="NN">protein-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AP-1</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">IL-6</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-IL6</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@52@@oe@16-12-2010
870963606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">EMSAs</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">IL-10</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="JJ">LPS-induced</tok> <tok cat="NN">AP-1</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
870963607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">inhibiting</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><cons cat="ADVP"><tok cat="RB">slightly</tok> <tok cat="RBR">more</tok></cons> <tok cat="JJ">pronounced</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
870963608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i56" role="SBJ"><cons cat="NP"><tok cat="NN">Downregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">LPS-induced</tok> <tok cat="NN">AP-1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">accompanied</tok><cons cat="NP" ref="i56" null="NONE"></cons><cons cat="PP" ref="i58" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <tok cat="RB">thus</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">possibly</tok></cons> <tok cat="VBN">explained</tok><cons cat="NP" ref="i56" null="NONE"></cons><cons cat="PP" ref="i58" null="RNR"></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP" id="i58"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="VBN">reduced</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="NN">mRNA</tok> <tok cat="NN">level</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="JJ">major</tok> <tok cat="NNS">components</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">AP-1</tok> <tok cat="NN">complex</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="RB">namely</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-jun</tok></cons></cons> <cons cat="SBAR"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i59" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">determined</tok> <cons cat="NP" ref="i59" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">Northern</tok> <tok cat="NNS">experiments</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
870963609@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i60" role="SBJ"><cons cat="NP"><tok cat="NN">Binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-IL6</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i60" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">IL-4</tok></cons></cons></cons></cons></cons> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-10</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">effect</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
870963610@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i61" role="SBJ"><tok cat="NN">NF-IL6</tok> <tok cat="NN">mRNA</tok> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">affected</tok> <cons cat="NP" ref="i61" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">IL-10</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-4</tok></cons> <cons cat="VP"><tok cat="VBZ">affects</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBG">preexisting</tok> <tok cat="NN">NF-IL6</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
870963611@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">Neither</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons> <tok cat="CC">nor</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="JJ">LPS-induced</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
870963612@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <tok cat="NN">agreement</tok> <tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">finding</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Northern</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i63"><tok cat="WRB">where</tok></cons> <cons cat="S"><cons cat="NP" id="i64" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p65</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p105</tok></cons></cons> <tok cat="NN">mRNA</tok> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">determined</tok><cons cat="NP" ref="i64" null="NONE"></cons><cons cat="ADVP" ref="i63" null="T"></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i65" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">components</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">affected</tok> <cons cat="NP" ref="i65" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">IL-10</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
870963613@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">neither</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons> <tok cat="CC">nor</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">any</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">I-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="SBAR"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i66" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">determined</tok> <cons cat="NP" ref="i66" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">Northern</tok> <tok cat="NNS">experiments</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
870963614@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">IL-10</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">similarly</tok></cons> <cons cat="VP"><tok cat="VBP">affect</tok> <cons cat="NP"><tok cat="NN">IL-6</tok> <tok cat="NN">expression</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
870963615@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons> <cons cat="NP" id="i67" role="SBJ"><tok cat="DT">this</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">accompanied</tok> <cons cat="NP" ref="i67" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">reduction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-IL6</tok></cons></cons> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons> <cons cat="SBAR"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-10</tok></cons> <cons cat="ADVP"><tok cat="RB">only</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
870963701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Interleukin-7</tok></cons> <tok cat="NN">signaling</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">precursor</tok> <tok cat="JJ">acute</tok> <tok cat="JJ">lymphoblastic</tok> <tok cat="NN">leukemia</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">BAF3</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT5</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">interleukin-7</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha</tok> <tok cat="NN">chain</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@29@@oe@16-12-2010
870963702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Interleukin-7</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-7</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">proliferation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">normal</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">leukemic</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><cons cat="NP" syn="COOD" id="i2"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">B</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><cons cat="NP" id="i1"><tok cat="NN">cell</tok> <tok cat="NNS">precursors</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@16-12-2010
870963703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i13" role="SBJ"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">JAK/STAT</tok> <tok cat="NN">pathway</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-7R</tok></cons> <tok cat="NN">signaling</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
870963704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NNS">complexes</tok></cons></cons> <cons cat="S"><cons cat="NP" ref="i4" id="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">formed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">precursor</tok> <tok cat="JJ">acute</tok> <tok cat="JJ">lymphoblastic</tok> <tok cat="NN">leukemia</tok> <tok cat="LRB">(</tok><tok cat="NN">BCP-ALL</tok><tok cat="RRB">)</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IL-7</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
870963705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Gel</tok> <tok cat="NN">retardation</tok> <tok cat="NNS">assays</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">STAT-binding</tok> <tok cat="NNS">oligonucleotides</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-7</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">formation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">major</tok> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NNS">complexes</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">BCP-ALL</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
870963706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Supershifts</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">anti-STAT</tok> <tok cat="NNS">antibodies</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><tok cat="DT">these</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">STAT1</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT5</tok> <tok cat="NNS">complexes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
870963707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">This</tok> <tok cat="NN">pattern</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">STAT</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">seen</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="NN">BCP-ALL</tok> <tok cat="NNS">cases</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">respond</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IL-7</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">proliferation</tok> <tok cat="NNS">assays</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
870963708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-7</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><tok cat="NN">STAT/DNA</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">BCP-ALL</tok> <tok cat="NNS">cases</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" id="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">failed</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">proliferate</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IL-7</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-7R</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">JAK/STAT</tok> <tok cat="NN">pathway</tok></cons> <cons cat="ADVP"><tok cat="FW">per</tok> <tok cat="FW">se</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">sufficient</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">proliferation</tok> <tok cat="NN">induction</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
870963709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">contribution</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">cytoplasmic</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-7</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha</tok> <tok cat="NN">chain</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-7R</tok> <tok cat="NN">alpha</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NNS">transfectants</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">pro-B</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NNS">BAF3</tok></cons></cons></cons></cons> <cons cat="SBAR" id="i12" null="ICH"></cons></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">made</tok> <cons cat="NP" ref="i11" null="NONE"></cons></cons></cons><cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="NN">express</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">chimeric</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="VP"><tok cat="VBG">consisting</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">extracellular</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">granulocyte</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">G-CSF-R</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transmembrane</tok> <cons cat="NP" ref="i14" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <cons cat="NP" ref="i14" null="RNR"></cons></cons><cons cat="NP" id="i14"><tok cat="NNS">domains</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">IL-7R</tok> <tok cat="NN">alpha</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@63@@oe@16-12-2010
870963710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">chimeric</tok> <tok cat="NN">G-CSF-R/IL-7R</tok> <tok cat="NN">alpha</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">G-CSF</tok></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">full</tok></cons> <cons cat="ADJP"><tok cat="JJ">proliferative</tok></cons></cons> <tok cat="NN">response</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">JAK1</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">JAK2</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
870963711@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Major</tok> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NNS">complexes</tok></cons></cons> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">G-CSF-R/IL-7R</tok> <tok cat="NN">alpha</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT1</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">STAT5</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" id="i15" role="SBJ"><cons cat="NP"><tok cat="DT">some</tok> <tok cat="NN">formation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">STAT3-containing</tok> <tok cat="NNS">complexes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">seen</tok><cons cat="NP" ref="i15" null="NONE"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
870963712@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">establish</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i16" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT5</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">possibly</tok></cons> <cons cat="NP"><tok cat="NN">STAT3</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i16" null="NONE"></cons><cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">precursor</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IL-7</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
870963713@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">data</tok></cons> <cons cat="ADVP"><tok cat="RB">further</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i17" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-7R</tok> <tok cat="NN">alpha</tok> <tok cat="NNS">chains</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">directly</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i17" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">JAKs</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">STATs</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">major</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">proliferative</tok> <tok cat="NN">signaling</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">precursor</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
872338701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Gene</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">G-protein-coupled</tok> <tok cat="NN">chemoattractant</tok> <tok cat="NNS">receptors</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@9@@oe@16-12-2010
872338702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NNS">Receptors</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">leukocyte</tok> <tok cat="NNS">chemoattractants</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><tok cat="NNS">chemokines</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">traditionally</tok></cons> <cons cat="VP"><tok cat="VBN">considered</tok> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">responsible</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">special</tok> <tok cat="JJ">leukocyte</tok> <tok cat="NNS">functions</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">chemotaxis</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">degranulation</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">release</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">superoxide</tok> <tok cat="NNS">anions</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@34@@oe@16-12-2010
872338703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Recently</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i2" role="SBJ"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="JJ">G-protein-coupled</tok> <tok cat="NN">serpentine</tok> <tok cat="NNS">receptors</tok></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">transduce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">signals</tok></cons> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="NN">gene</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">translation</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">leukocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
872338704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="NN">Transcription</tok> <tok cat="NNS">factors</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NNS">chemoattractants</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">physiologically</tok> <tok cat="JJ">relevant</tok></cons> <tok cat="NNS">concentrations</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
872338705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">receptors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">G-protein</tok></cons> <tok cat="NN">coupling</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NNS">kinases</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
872338706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">underlying</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="VP"><tok cat="VBP">appear</tok> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">different</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="VP"><tok cat="VBN">utilized</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RBR">better</tok> <tok cat="VBN">characterized</tok></cons> <tok cat="NN">cytokine</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">immediate-early</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
872338707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">Chemoattractants</tok></cons> <cons cat="VP"><tok cat="VBP">stimulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="JJ">several</tok> <cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NNS">cytokines</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">chemokines</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">turn</tok></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">respective</tok> <tok cat="NNS">receptors</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">initiate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">autocrine</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">persistent</tok></cons> <cons cat="NP"><tok cat="NN">cytokine</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">chemokine</tok></cons> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
872593901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NNS">stages</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">B-cell</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><cons cat="NP" id="i1"><tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
872593902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">distinct</tok> <tok cat="NNS">sets</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">genes</tok></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">stages</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">B-lymphocyte</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T-lymphocyte</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons><cons cat="NP" id="i3"><tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">controlled</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@16-12-2010
872593903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">recent</tok> <tok cat="NNS">studies</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">described</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">interactions</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">new</tok> <tok cat="NNS">insights</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">mechanisms</tok></cons> <cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
872593904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="VP"><tok cat="VBP">include</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">assembly</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJR">higher</tok> <tok cat="NN">order</tok> <tok cat="NN">nucleoprotein</tok> <tok cat="NNS">complexes</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="JJ">protein-protein</tok> <tok cat="NNS">interactions</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">enhance</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">functional</tok> <tok cat="NN">specificity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NNS">regulators</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
872869401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">X</tok> <tok cat="NN">inactivation</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">female</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">hypomelanosis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Ito</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">balanced</tok> <tok cat="NN">X</tok><tok cat="COLON">;</tok><tok cat="CD">17</tok> <tok cat="NN">translocation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="NN">disomy</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Xp</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@26@@oe@16-12-2010
872869402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="NN">X</tok> <tok cat="NN">inactivation</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">performed</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">normal</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">hypopigmented</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><cons cat="NP" id="i2"><tok cat="NN">skin</tok> <tok cat="NNS">samples</tok></cons></cons> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">female</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">hypomelanosis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Ito</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">balanced</tok> <tok cat="JJ">whole</tok> <tok cat="NN">arm</tok> <tok cat="NN">X</tok><tok cat="COLON">;</tok><tok cat="CD">17</tok> <tok cat="NN">translocation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@30@@oe@16-12-2010
872869403@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Severe</tok> <tok cat="NN">skewing</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">X</tok></cons> <tok cat="NN">inactivation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">inactivity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">intact</tok> <cons cat="NP"><tok cat="NN">X</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">blood</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">cultures</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">types</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">skin</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">DNA</tok></cons> <cons cat="VP"><tok cat="VBN">prepared</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><cons cat="ADVP"><tok cat="RB">directly</tok></cons> <tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">hypopigmented</tok> <tok cat="NN">skin</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">significant</tok> <tok cat="NN">inactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">translocated</tok> <tok cat="NN">X</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="JJ">inconsistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">usual</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">phenotypic</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">X</tok><tok cat="COLON">;</tok><tok cat="JJ">autosome</tok> <tok cat="NNS">translocations</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@55@@oe@16-12-2010
872869404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">dual</tok> <tok cat="NN">colour</tok> <tok cat="NN">FISH</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">centromere</tok> <tok cat="JJ">specific</tok> <tok cat="NNS">probes</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">chromosomes</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">X</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">17</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">breakpoints</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">both</tok> <cons cat="NP"><tok cat="NNS">chromosomes</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">lie</tok> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">alphoid</tok> <tok cat="NNS">arrays</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">making</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">interruption</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">locus</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">either</tok> <tok cat="NN">chromosome</tok></cons></cons></cons></cons></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">unlikely</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
872869405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">While</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">partial</tok></cons> <cons cat="ADJP"><tok cat="JJ">variable</tok></cons></cons> <tok cat="NN">monosomy</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">loci</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="NN">17p</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok><tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">excluded</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">contributing</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phenotype</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">patient</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">argued</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">major</tok></cons> <cons cat="ADJP"><tok cat="JJ">likely</tok></cons></cons> <tok cat="NN">factor</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">partial</tok></cons> <cons cat="ADJP"><tok cat="JJ">functional</tok></cons></cons> <tok cat="NN">disomy</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">sequences</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Xp</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NNS">lineages</tok></cons></cons></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" id="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">failed</tok> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inactivate</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">intact</tok> <cons cat="NP"><tok cat="NN">X</tok> <tok cat="NN">chromosome</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@16-12-2010
873478701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NN">Stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">antigen</tok> <tok cat="JJ">receptor-CD3</tok> <tok cat="NN">complex</tok></cons> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphatase</tok></cons> <tok cat="NN">inhibitor</tok></cons> <cons cat="NP"><tok cat="NN">pervanadate</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD45</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><tok cat="CD">two</tok> <tok cat="JJ">interconnected</tok> <cons cat="NP"><tok cat="NN">Lck/Fyn-</tok> <cons cat="ADJP" null="QSTN"></cons><cons cat="NP" ref="i2" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">zap-70-dependent</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><cons cat="NP" id="i2"><tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@32@@oe@16-12-2010
873478702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphatase</tok></cons> <tok cat="JJ">specific</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="NP"><tok cat="NN">pervanadate</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potent</tok> <tok cat="NN">activator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">downstream</tok> <tok cat="NNS">events</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok> <tok cat="NN">cascade</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@26@@oe@16-12-2010
873478703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CD45-</tok> <cons cat="ADJP" null="QSTN"></cons><cons cat="NP" ref="i4" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">CD3-negative</tok> <cons cat="NP" ref="i4" null="RNR"></cons></cons><cons cat="NP" id="i4"><tok cat="NNS">variants</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="JJ">leukemic</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">line</tok></cons></cons></cons></cons></cons></cons> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">different</tok></cons> <cons cat="ADJP"><tok cat="JJ">biochemical</tok></cons></cons> <tok cat="NNS">events</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">pervanadate</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">appeared</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">surface</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="NN">CD45</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">CD3</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
873478704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">CD45-dependent</tok> <tok cat="NNS">events</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Shc</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons><tok cat="RRB">)</tok></cons></cons><cons cat="NP" ref="i6" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">activator</tok> <tok cat="NN">protein-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AP-1</tok></cons><tok cat="RRB">)</tok></cons></cons><cons cat="NP" ref="i6" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">interleukin-2</tok> <tok cat="LRB">(</tok><tok cat="NN">IL-2</tok><tok cat="RRB">)</tok> <tok cat="NN">promoter</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">CD69</tok></cons> <cons cat="NP" ref="i7" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD25</tok></cons> <cons cat="NP" ref="i7" null="RNR"></cons></cons><cons cat="NP" id="i7"><tok cat="NN">surface</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">paralleled</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NNS">kinases</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">lck</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">fyn</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@53@@oe@16-12-2010
873478705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">calcium</tok> <tok cat="NN">influx</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">CD3-dependent</tok> <tok cat="NN">event</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">paralleled</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">zap-70</tok></cons> <tok cat="NN">activation</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
873478706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">antigen</tok> <tok cat="JJ">receptor-CD3</tok> <tok cat="LRB">(</tok><tok cat="JJ">TcR-CD3</tok><tok cat="RRB">)</tok> <tok cat="NN">complex</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">functionally</tok></cons> <cons cat="VP"><tok cat="VBN">linked</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">different</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PTK</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">modules</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">separate</tok></cons> <cons cat="ADJP"><tok cat="JJ">specific</tok></cons></cons> <tok cat="NNS">functions</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">CD45</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">regulator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">coupling</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
873956301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NN">adhesion</tok> <tok cat="NN">molecule</tok></cons> <tok cat="NN">induction</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@10@@oe@16-12-2010
873956302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NN">adhesion</tok> <tok cat="NNS">molecules</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor-alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNF</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">occur</tok> <cons cat="ADVP"><tok cat="RB">independently</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PTK</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">upregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">vascular</tok> <tok cat="NN">cell</tok> <tok cat="NN">adhesion</tok> <tok cat="NN">molecule</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">VCAM-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-4</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-4</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@56@@oe@16-12-2010
873956303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">PTK</tok></cons> <tok cat="NNS">inhibitors</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">herbimycin</tok> <tok cat="NN">A</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">genistein</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">suppress</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">endothelial</tok> <cons cat="NP"><tok cat="NN">VCAM-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">E-selectin</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">subsequent</tok></cons> <cons cat="ADJP"><tok cat="JJ">monocytic</tok></cons></cons> <tok cat="NN">cell</tok> <tok cat="NN">adhesion</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">TNF</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
873956304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Inhibition</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="VP"><tok cat="VBG">following</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PTK</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mobilization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">VCAM-1</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
873956305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">implications</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">pathophysiological</tok> <tok cat="NNS">conditions</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">involve</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">upregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="PP"><tok cat="FW">e.g.</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">inflammation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">atherosclerosis</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
874166401@GENIA Treebank@formal@@1@S@<cons cat="NP"><tok cat="LRB">[</tok><cons cat="NP"><tok cat="NN">NGFI-B/nur77</tok> <tok cat="NN">family</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">apoptosis</tok></cons></cons></cons><tok cat="RRB">]</tok></cons>@@@@1@8@@oe@16-12-2010
874166402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NGFI-B/nur77</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">member</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">steroid</tok> <tok cat="NN">receptor</tok> <tok cat="NN">superfamily</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
874166403@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">NGFI-B/nur77</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">related</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="VP"><tok cat="VBP">constitute</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">family</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">NGFI-B/nur77</tok> <tok cat="NN">family</tok></cons> <cons cat="VP"><tok cat="VBZ">consists</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok> <tok cat="NNS">subtypes</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">named</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" syn="COOD" role="PRD"><cons cat="NP"><tok cat="NN">nur77</tok> <tok cat="NN">alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">nur77</tok> <tok cat="NN">beta</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">nur77</tok> <tok cat="NN">gamma</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
874166404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">cloned</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">nur77</tok> <tok cat="NN">beta</tok> <tok cat="NN">cDNA</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">called</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NN">TINUR</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
874166405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NGFI-B/nur77</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">TCR-mediated</tok> <tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NNS">hybridomas</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">reports</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">nur77</tok> <tok cat="JJ">knock-out</tok> <tok cat="NNS">mice</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">nur77</tok> <tok cat="JJ">dominant</tok> <tok cat="JJ">negative</tok> <tok cat="JJ">transgenic</tok> <tok cat="NNS">mice</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <tok cat="JJ">functional</tok> <tok cat="NN">redundancy</tok></cons> <cons cat="PP"><tok cat="IN">among</tok> <cons cat="NP"><tok cat="NN">NGFI-B/nur77</tok> <tok cat="NN">family</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
874166406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NGFI-B/nur77</tok></cons> <cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">response</tok> <tok cat="NN">element</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">monomer</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">heterodimer</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">retinoid</tok> <tok cat="NN">X</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RXR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
874166407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="VBG">Assuming</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">9-cis-retinoic</tok> <tok cat="NN">acid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">9-cis-RA</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><tok cat="JJ">TCR-mediated</tok> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NNS">nur77</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">cause</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">monomer</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">absence</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">9-cis-RA</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">heterodimer</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">RXR</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">presence</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">9-cis-RA</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
874708301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Transcription</tok> <tok cat="JJ">specific</tok> <tok cat="NNS">differences</tok></cons> <cons cat="VP"><tok cat="VBN">visualized</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">fluorescence</tok> <tok cat="FW">in</tok> <tok cat="FW">situ</tok> <tok cat="NN">hybridization</tok> <tok cat="NN">pattern</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">interphase</tok> <tok cat="NNS">nuclei</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@16-12-2010
874708302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Application</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="LQT">"</tok><tok cat="NN">formamide</tok> <tok cat="JJ">free</tok><tok cat="RQT">"</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="ADJP"><tok cat="LQT">"</tok><tok cat="NN">material</tok> <tok cat="NN">preserving</tok><tok cat="RQT">"</tok></cons></cons> <tok cat="FW">in</tok> <tok cat="FW">situ</tok> <tok cat="NN">hybridization</tok> <tok cat="NN">technique</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">cDNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">myf3</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">following</tok> <tok cat="NNS">results</tok><cons cat="S" ref="i30" null="ICH"></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="S" id="i30"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Human</tok> <tok cat="NN">rhabdomyosarcoma</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">characterized</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">high</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">myf3</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="NP"><tok cat="JJ">intensive</tok> <tok cat="NN">hybridization</tok> <tok cat="NNS">signals</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">interphase</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@48@@oe@16-12-2010
874708303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">RNase</tok></cons> <tok cat="NN">treatment</tok></cons> <cons cat="ADJP"><tok cat="JJ">prior</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">hybridization</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">considerably</tok></cons> <cons cat="VP"><tok cat="VBZ">reduces</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">size</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NNS">signals</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
874708304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">comparison</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="VBN">isolated</tok> <tok cat="NNS">nuclei</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">need</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">exists</tok><cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="ADVP"><tok cat="RB">barely</tok></cons> <cons cat="NP"><tok cat="NN">hybridization</tok> <tok cat="NNS">signals</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
874708305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Correspondingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">RNase</tok></cons> <tok cat="NN">treatment</tok></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">hybridization</tok> <tok cat="NN">pattern</tok></cons></cons> <cons cat="ADVP"><tok cat="IN">at</tok> <tok cat="DT">all</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
874708306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">conclusion</tok></cons></cons> <cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="VBN">increased</tok> <tok cat="NN">transcription</tok> <tok cat="NN">efficiency</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cell</tok> <tok cat="NN">type</tok> <tok cat="JJ">specific</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">accompanied</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJR">higher</tok> <tok cat="NN">hybridization</tok> <tok cat="NN">accessibility</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">corresponding</tok> <tok cat="NN">cell</tok> <tok cat="NNS">nuclei</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
875193701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">monocytic</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="FW">Treponema</tok> <tok cat="FW">pallidum</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="FW">Borrelia</tok> <tok cat="FW">burgdorferi</tok></cons></cons> <tok cat="NNS">lipoproteins</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">synthetic</tok> <tok cat="NNS">lipopeptides</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">proceeds</tok> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">pathway</tok></cons> <cons cat="ADJP"><tok cat="JJ">distinct</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">lipopolysaccharide</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activator</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
875193702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">There</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="VBG">increasing</tok> <tok cat="NN">evidence</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">lipoproteins</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="FW">Treponema</tok> <tok cat="FW">pallidum</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="FW">Borrelia</tok> <tok cat="FW">burgdorferi</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><tok cat="JJ">key</tok> <tok cat="JJ">inflammatory</tok> <tok cat="NNS">mediators</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">syphilis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Lyme</tok> <tok cat="NN">disease</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
875193703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">principal</tok> <tok cat="NN">objective</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="S" role="PRD"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">identify</tok> <cons cat="ADVP"><tok cat="RBR">more</tok> <tok cat="RB">precisely</tok></cons> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">similarities</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">divergences</tok></cons></cons> <cons cat="PP"><tok cat="IN">among</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><cons cat="NP"><cons cat="NP"><tok cat="NN">lipopolysaccharide</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LPS</tok></cons><tok cat="RRB">)</tok></cons><tok cat="COLON">-</tok></cons> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">lipoprotein-lipopeptide-induced</tok></cons></cons> <tok cat="JJ">immune</tok> <tok cat="NN">cell</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">events</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
875193704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Like</tok> <cons cat="NP"><tok cat="NN">LPS</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">purified</tok> <tok cat="JJ">native</tok> <tok cat="FW">B.</tok> <tok cat="FW">burgdorferi</tok> <tok cat="NN">OspA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">synthetic</tok> <tok cat="NNS">analogs</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">OspA</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">OspB</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="FW">T.</tok> <tok cat="FW">pallidum</tok> <tok cat="NNS">lipoproteins</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Tpp47</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Tpp17</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="NP"><tok cat="DT">all</tok></cons></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">THP-1</tok> <tok cat="JJ">human</tok> <tok cat="NN">monocytoid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
875193705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Acylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">OspA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">synthetic</tok> <tok cat="NNS">peptides</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">requisite</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
875193706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Polymyxin</tok> <tok cat="NN">B</tok></cons> <cons cat="VP"><tok cat="VBD">abrogated</tok> <cons cat="NP"><cons cat="NP"><tok cat="RB">only</tok> <tok cat="DT">the</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">LPS</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
875193707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">70Z/3-derived</tok> <tok cat="NN">pre-B-cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><tok cat="CC">either</tok> <cons cat="VP"><tok cat="VBG">lacking</tok> <cons cat="NP" ref="i32" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP" ref="i32" null="RNR"></cons></cons></cons><cons cat="NP" id="i32"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">CD14</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">LPS</tok> <tok cat="NN">receptor</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">CD14</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">imparted</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">responsiveness</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">LPS</tok></cons></cons> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">OspA</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">spirochetal</tok> <tok cat="NNS">lipopeptides</tok></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" id="i100" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">assessed</tok> <cons cat="NP" ref="i100" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">surface</tok> <tok cat="NN">immunoglobulin</tok> <tok cat="NN">M</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@16-12-2010
875193708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i33" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">biological</tok> <tok cat="NN">relevance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">observation</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="FW">T.</tok> <tok cat="FW">pallidum</tok> <tok cat="NNS">lipoproteins-lipopeptides</tok></cons> <cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">cytokine</tok></cons></cons> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">supported</tok> <cons cat="NP" ref="i33" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok> <cons cat="S" ref="i34" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">synthetic</tok> <tok cat="NNS">analogs</tok></cons></cons> <cons cat="S" id="i34"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">promote</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">replication</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">chronically</tok> <tok cat="JJ">infected</tok></cons> <tok cat="NN">U1</tok> <tok cat="NN">monocytoid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">observations</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potential</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i35"><tok cat="WRB">whereby</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">a</tok> <tok cat="JJ">syphilitic</tok> <tok cat="NN">chancre</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">serve</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cofactor</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">transmission</tok></cons></cons></cons></cons><cons cat="ADVP" ref="i35" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@65@@oe@16-12-2010
875193709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">combined</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">lend</tok> <cons cat="NP"><tok cat="JJ">additional</tok> <tok cat="NN">support</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">proposal</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="JJ">spirochetal</tok> <tok cat="NNS">lipoproteins</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">LPS</tok></cons></cons> <cons cat="VP"><tok cat="VBP">initiate</tok> <cons cat="NP"><tok cat="NN">monocyte</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <tok cat="NNS">events</tok></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i36" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="ADVP"><tok cat="RB">ultimately</tok></cons> <cons cat="VP"><tok cat="VBP">converge</tok> <cons cat="S"><cons cat="NP" ref="i36" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">produce</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">qualitatively</tok> <tok cat="JJ">similar</tok></cons> <tok cat="JJ">cellular</tok> <tok cat="NNS">responses</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
875485501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Precise</tok> <tok cat="NN">alignment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">sites</tok></cons></cons> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">mu</tok> <tok cat="NN">enhancer</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
875485502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i44" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">lymphocyte-specific</tok> <tok cat="NN">immunoglobulin</tok> <tok cat="NN">mu</tok> <tok cat="JJ">heavy-chain</tok> <tok cat="NN">gene</tok> <tok cat="JJ">intronic</tok> <tok cat="NN">enhancer</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i44" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">multiple</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
875485503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i45" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="ADJP"><tok cat="RB">previously</tok> <tok cat="VBN">defined</tok></cons> <tok cat="JJ">minimal</tok> <tok cat="NN">enhancer</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">muA</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">muE3</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">muB</tok></cons></cons> <tok cat="NNS">sites</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">transactivated</tok> <cons cat="NP" ref="i45" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ETS-domain</tok> <tok cat="NNS">proteins</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PU.1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Ets-1</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">nonlymphoid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
875485504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i46" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">core</tok> <tok cat="NNS">GGAAs</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">muA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">muB</tok></cons></cons> <tok cat="NNS">sites</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">separated</tok> <cons cat="NP" ref="i46" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="CD">30</tok> <tok cat="NNS">nucleotides</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">ETS</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBP">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">these</tok> <tok cat="JJ">same</tok> <tok cat="NN">side</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DNA</tok> <tok cat="NN">helix</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
875485505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i48" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">tested</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">necessity</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">appropriate</tok> <tok cat="JJ">spatial</tok> <tok cat="NN">alignment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">elements</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i48" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">mutated</tok> <tok cat="NNS">enhancers</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">altered</tok> <tok cat="NNS">spacings</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
875485506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><cons cat="NP"><tok cat="CD">4-</tok></cons> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">10-bp</tok></cons></cons> <tok cat="NN">insertion</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">muE3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">muB</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">inactivated</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mu</tok> <tok cat="NN">enhancer</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">S194</tok> <tok cat="NN">plasma</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Ets-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">PU.1</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">muE3-binding</tok> <tok cat="NN">protein</tok> <tok cat="NN">TFE3</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="UCP" syn="COOD"><cons cat="ADVP"><tok cat="RB">alone</tok></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">pairwise</tok> <tok cat="NNS">combinations</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
875485507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">Circular</tok> <tok cat="NN">permutation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">phasing</tok></cons></cons> <tok cat="NNS">analyses</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">PU.1</tok> <tok cat="NN">binding</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">TFE3</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">Ets-1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">bends</tok> <cons cat="NP"><tok cat="NN">mu</tok> <tok cat="NN">enhancer</tok> <tok cat="NN">DNA</tok></cons> <cons cat="PP"><tok cat="IN">toward</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">major</tok> <tok cat="NN">groove</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
875485508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">propose</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">requirement</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">precise</tok> <tok cat="NN">spacing</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">muA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">muB</tok></cons></cons> <tok cat="NNS">elements</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="PP" role="PRD"><tok cat="JJ">due</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">part</tok></cons></cons> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">directed</tok> <tok cat="NN">DNA</tok> <tok cat="NN">bend</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">PU.1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
875556601@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Peptide</tok> <tok cat="NN">vaccination</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">lead</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="VBN">enhanced</tok> <tok cat="NN">tumor</tok> <tok cat="NN">growth</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">tolerance</tok> <tok cat="NN">induction</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
875556602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Vaccination</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">synthetic</tok> <tok cat="NNS">peptides</tok></cons> <cons cat="VP"><tok cat="VBG">representing</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">cytotoxic</tok> <tok cat="NN">T</tok> <tok cat="NN">lymphocyte</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CTL</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">epitopes</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">lead</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">protective</tok> <tok cat="JJ">CTL-mediated</tok> <tok cat="NN">immunity</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">tumors</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NNS">viruses</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
875556603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">vaccination</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">CTL</tok> <tok cat="NN">epitope</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">adenovirus</tok> <tok cat="NN">type</tok> <tok cat="CD">5</tok> <tok cat="NN">E1A-region</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Ad5E1A234-243</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i49"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i49" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">serve</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">target</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">tumor-eradicating</tok> <tok cat="NN">CTL</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">enhances</tok> <cons cat="NP" ref="i50" null="RNR"></cons></cons><cons cat="CONJP"><tok cat="RB">rather</tok> <tok cat="IN">than</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP" ref="i50" null="RNR"></cons></cons></cons><cons cat="NP" id="i50"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">growth</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">Ad5E1A-expressing</tok> <tok cat="NNS">tumors</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
875556604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i51" role="SBJ"><cons cat="NP"><tok cat="DT">This</tok> <tok cat="JJ">adverse</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">peptide</tok> <tok cat="NN">vaccination</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok></cons> <cons cat="VP"><tok cat="VBN">evoked</tok><cons cat="NP" ref="i51" null="NONE"></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">required</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">low</tok> <tok cat="NNS">doses</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">peptide</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">10</tok> <tok cat="NNS">micrograms</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">achieved</tok> <cons cat="NP" ref="i51" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">mode</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">peptide</tok> <tok cat="NN">delivery</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i52"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i52" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><tok cat="JJ">protective</tok> <tok cat="JJ">T-cell-mediated</tok> <tok cat="NN">immunity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">models</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
875556605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i53" role="SBJ"><tok cat="JJ">Ad5E1A-specific</tok> <tok cat="NN">CTL</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">no</tok> <tok cat="RB">longer</tok></cons> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" ref="i53" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NNS">mice</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">injection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Ad5E1A-peptide</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">tolerization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">Ad5E1A-specific</tok> <tok cat="NN">CTL</tok> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">causes</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">enhanced</tok> <tok cat="NN">tumor</tok> <tok cat="NN">outgrowth</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
875556606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <tok cat="NN">contrast</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">peptide</tok> <tok cat="NN">vaccination</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">immunization</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">adenovirus</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP"><tok cat="NN">Ad5E1A</tok></cons></cons><tok cat="COMMA">,</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><tok cat="JJ">Ad5E1A-specific</tok> <tok cat="NN">immunity</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">prevented</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">outgrowth</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">Ad5E1A-expressing</tok> <tok cat="NNS">tumors</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
875556607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">immunization</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">synthetic</tok> <tok cat="NNS">peptides</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">lead</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">elimination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">anti-tumor</tok> <tok cat="NN">CTL</tok> <tok cat="NNS">responses</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
875556608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">design</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">safe</tok> <tok cat="JJ">peptide-based</tok> <tok cat="NNS">vaccines</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">tumors</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">allogeneic</tok> <tok cat="NN">organ</tok> <tok cat="NNS">transplants</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">T-cell-mediated</tok> <tok cat="JJ">autoimmune</tok> <tok cat="NNS">diseases</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
875557401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Transgenic</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PML/RARalpha</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">impairs</tok> <cons cat="NP"><tok cat="NN">myelopoiesis</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@7@@oe@16-12-2010
875557402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">translocation</tok></cons> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">promyelocytic</tok> <tok cat="NN">leukemia</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">rearranges</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">promyelocytic</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">gene</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PML</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="CD">15</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RARalpha</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="CD">17</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
875557403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBZ">yields</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">fusion</tok> <tok cat="NN">transcript</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">PML/RARalpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">reported</tok> <tok cat="JJ">dominant</tok> <tok cat="JJ">negative</tok> <tok cat="NNS">functions</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">absence</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">hormone</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
875557404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i44" role="SBJ"><cons cat="NP"><tok cat="JJ">Clinical</tok> <tok cat="NNS">remissions</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">all-trans</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RA</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">treatment</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">promyelocytic</tok> <tok cat="NN">leukemia</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">linked</tok> <cons cat="NP" ref="i44" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PML/RARalpha</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">leukemic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
875557405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">evaluate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">PML/RARalpha</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">myelopoiesis</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i45" role="SBJ"><cons cat="NP"><tok cat="JJ">transgenic</tok> <tok cat="NNS">mice</tok></cons> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP"><tok cat="NN">PML/RARalpha</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">engineered</tok><cons cat="NP" ref="i45" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
875557406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i46" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">full-length</tok> <tok cat="NN">PML/RARalpha</tok> <tok cat="NN">cDNA</tok></cons> <cons cat="VP"><tok cat="VBN">driven</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="NN">CD11b</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i46" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">transgenic</tok> <tok cat="NNS">mice</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
875557407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i47" role="SBJ"><tok cat="NN">Expression</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">confirmed</tok> <cons cat="NP" ref="i47" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">bone</tok> <tok cat="NN">marrow</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">reverse</tok> <tok cat="NN">transcription</tok> <tok cat="NN">PCR</tok> <tok cat="NN">assay</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
875557408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Basal</tok> <tok cat="JJ">total</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">white</tok> <tok cat="NN">blood</tok> <tok cat="NN">cell</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">granulocyte</tok></cons></cons> <tok cat="NNS">counts</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="ADVP"><tok cat="RB">appreciably</tok></cons> <cons cat="VP"><tok cat="VB">differ</tok> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="UCP" syn="COOD"><cons cat="ADJP"><tok cat="NN">PML/RARalpha</tok> <tok cat="JJ">transgenic</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">control</tok></cons></cons> <tok cat="NNS">mice</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
875557409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Cell</tok> <tok cat="NN">sorter</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">CD11b+</tok> <tok cat="NN">bone</tok> <tok cat="NN">marrow</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><tok cat="JJ">similar</tok> <tok cat="JJ">CD11b+</tok> <tok cat="NNS">populations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="UCP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">transgenic</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">control</tok></cons></cons> <tok cat="NNS">mice</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
875557410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="JJ">clonal</tok> <tok cat="NN">growth</tok> <tok cat="NNS">assays</tok></cons> <cons cat="VP"><tok cat="VBN">performed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="UCP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">transgenic</tok></cons> <tok cat="CC">versus</tok> <cons cat="NP"><tok cat="NN">control</tok></cons></cons> <tok cat="NNS">mice</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">marked</tok> <tok cat="NN">reduction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NNS">progenitors</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="RB">especially</tok> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="VP"><tok cat="VBG">responding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">granulocyte/ macrophage</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
875557411@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Granulocyte/macrophage</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">kit</tok> <tok cat="NN">ligand</tok> <tok cat="NN">cotreatment</tok></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">overcome</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">inhibition</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
875557412@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i48" role="SBJ"><cons cat="NP"><tok cat="JJ">Impaired</tok> <tok cat="NN">myelopoiesis</tok></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="NP" ref="i48" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">stressing</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">mice</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">sublethal</tok> <tok cat="NN">irradiation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
875557413@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><tok cat="VBG">Following</tok> <cons cat="NP"><tok cat="NN">irradiation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i49" role="SBJ"><cons cat="ADJP"><tok cat="NN">PML/RARalpha</tok> <tok cat="JJ">transgenic</tok></cons> <tok cat="NNS">mice</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" ref="i49" id="i50" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" ref="i50" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NNS">controls</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP" adv="TMP"><tok cat="RBR">more</tok> <tok cat="RB">rapidly</tok></cons> <cons cat="VP"><tok cat="VBD">depressed</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="JJ">white</tok> <tok cat="NN">blood</tok> <tok cat="NN">cell</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">granulocyte</tok></cons></cons> <tok cat="NNS">counts</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
875557414@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">As</tok> <cons cat="S"><cons cat="NP" id="i51" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">expected</tok><cons cat="NP" ref="i51" null="NONE"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="QP"><tok cat="RB">nearly</tok> <tok cat="DT">all</tok></cons> <tok cat="NN">control</tok> <tok cat="NNS">mice</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">94.4</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">survived</tok> <cons cat="NP"><tok cat="NN">irradiation</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">yet</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">irradiation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">lethal</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">45.8</tok><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">PML/RARalpha</tok> <tok cat="JJ">transgenic</tok></cons> <tok cat="NNS">mice</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
875557415@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i52" role="SBJ"><tok cat="NN">Lethality</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i52" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RBR">more</tok> <tok cat="JJ">severe</tok></cons> <tok cat="NN">leukopenia</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="UCP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">transgenic</tok></cons> <tok cat="CC">versus</tok> <cons cat="NP"><tok cat="NN">control</tok></cons></cons> <tok cat="NNS">mice</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
875557416@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Retinoic</tok> <tok cat="NN">acid</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">irradiated</tok> <tok cat="NN">PML/RARalpha</tok> <tok cat="NNS">mice</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">enhanced</tok> <cons cat="NP"><tok cat="NN">granulocyte</tok> <tok cat="NN">recovery</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
875557417@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">abnormal</tok> <tok cat="NN">myelopoiesis</tok></cons> <cons cat="PP"><tok cat="IN">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">PML/RARalpha</tok> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">early</tok> <tok cat="NN">event</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">oncogenic</tok> <tok cat="NN">transformation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
875662201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Interaction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="JJ">lymphotropic</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok> <tok cat="NN">tax</tok> <tok cat="NN">transactivator</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">IIA</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
875662202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">Tax</tok> <tok cat="NN">protein</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">lymphotropic</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HTLV-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">40-kDa</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activator</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i31"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i31" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">critical</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HTLV-1</tok> <tok cat="NN">gene</tok> <tok cat="NN">regulation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">virus-induced</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">transformation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
875662203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i32" role="SBJ"><tok cat="NN">Tax</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="JJ">localized</tok> <cons cat="NP" ref="i32" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">site-specific</tok> <tok cat="NNS">activators</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">cyclic</tok> <tok cat="JJ">AMP-responsive</tok> <tok cat="JJ">element-binding</tok></cons> <tok cat="NN">protein</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">serum</tok> <tok cat="NN">response</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
875662204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">suggested</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">recruitment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Tax</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DNA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">positions</tok> <cons cat="NP"><tok cat="NN">Tax</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">basal</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">machinery</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
875662205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">On</tok> <tok cat="DT">the</tok> <tok cat="NN">basis</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">several</tok> <tok cat="JJ">independent</tok> <tok cat="NNS">assays</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">physical</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">functional</tok></cons></cons> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Tax</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">TFIIA</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
875662206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">First</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i33" role="SBJ"><tok cat="NN">Tax</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i33" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">interact</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="JJ">35-kDa</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">alpha</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">subunit</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TFIIA</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">yeast</tok> <tok cat="JJ">two-hybrid</tok> <tok cat="NN">interaction</tok> <tok cat="NN">system</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
875662207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Importantly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i34" role="SBJ"><cons cat="NP"><tok cat="CD">two</tok> <cons cat="ADJP"><tok cat="RB">previously</tok> <tok cat="VBN">characterized</tok></cons> <tok cat="NNS">mutants</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">point</tok> <tok cat="NNS">mutations</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Tax</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">M32</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Y196A</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">K197S</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">M41</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">H287A</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">P288S</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i35"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i35" id="i36" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i36" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">defective</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">Tax-activated</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unable</tok> <cons cat="S"><cons cat="NP" ref="i34" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">interact</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">TFIIA</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">assay</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
875662208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Second</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">a</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glutathione-S-transferase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GST</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="JJ">affinity-binding</tok> <tok cat="NN">assay</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">holo-TFIIA</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">GST-Tax</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="RB">20-fold</tok> <tok cat="JJR">higher</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">GST-Tax</tok> <tok cat="NN">M32</tok> <tok cat="NN">activation</tok> <tok cat="NN">mutant</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">GST</tok> <tok cat="NN">control</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
875662209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Third</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">a</tok> <tok cat="NN">coimmunoprecipitation</tok> <tok cat="NN">assay</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">HTLV-1-infected</tok> <tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i37" role="SBJ"><cons cat="NP"><tok cat="NN">Tax</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TFIIA</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">associated</tok><cons cat="NP" ref="i37" null="NONE"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
875662210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">TFIIA</tok></cons> <cons cat="VP"><tok cat="NNS">facilitates</tok> <cons cat="NP"><tok cat="NN">Tax</tok> <tok cat="NN">transactivation</tok></cons> <cons cat="ADVP" syn="COOD"><cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
875662211@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP"><tok cat="FW">In</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">transcription</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">reduced</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">Tax-activated</tok> <tok cat="NN">transcription</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="VP"><tok cat="VBN">depleted</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TFIIA</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
875662212@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">transfection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">TFIIA</tok> <tok cat="NN">expression</tok> <tok cat="NNS">vectors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">enhanced</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Tax-activated</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP"><tok cat="NN">HTLV-1</tok> <tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat-chloramphenicol</tok> <tok cat="NN">acetyltransferase</tok> <tok cat="NN">reporter</tok></cons> <tok cat="NN">construct</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
875662213@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Tax</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">TFIIA</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">Tax-mediated</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
875731601@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Alpha</tok> <tok cat="CD">4</tok> <tok cat="NN">beta</tok> <tok cat="CD">1</tok> <tok cat="LRB">(</tok><tok cat="NN">CD49d/CD29</tok><tok cat="RRB">)</tok> <tok cat="NN">integrin</tok></cons> <tok cat="NN">costimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">enhances</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="NP" ref="i1" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">cytokine</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><cons cat="NP" id="i1"><tok cat="NN">induction</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">absence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">altered</tok> <tok cat="NN">sensitivity</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">anti-CD3</tok></cons> <tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@29@@oe@16-12-2010
875731602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">integrin</tok> <tok cat="NN">alpha</tok> <tok cat="CD">4</tok> <tok cat="NN">beta</tok> <tok cat="CD">1</tok></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">costimulus</tok></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i9" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="NN">secretion</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2R</tok></cons> <tok cat="NN">expression</tok></cons></cons> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">enhanced</tok> <tok cat="NN">proliferation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">purified</tok><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i9" null="T"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@29@@oe@16-12-2010
875731603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="JJ">Similar</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="JJ">recombinant</tok> <tok cat="NN">vascular-cell</tok> <tok cat="NN">adhesion</tok> <tok cat="NN">molecule-1</tok><tok cat="COMMA">,</tok></cons> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i3"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">co-immobilized</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">anti-CD3</tok> <tok cat="NN">mAb</tok></cons></cons><cons cat="ADVP" ref="i3" null="T"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <tok cat="VBD">enhanced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-AT</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">determined</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">electromobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
875731604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">alpha</tok> <tok cat="CD">4</tok> <tok cat="NN">beta</tok> <tok cat="CD">1</tok></cons> <tok cat="NN">ligation</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <tok cat="NN">binding</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
875731605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">requirements</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">reflected</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">requirements</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">secretion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">multiple</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">granulocyte</tok> <tok cat="NN">macrophage-CSF</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
875731606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="RB">freshly</tok> <tok cat="VBN">isolated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="FW">in</tok> <cons cat="NP"><tok cat="JJ">vitro-cultured</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">require</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">costimulation</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok></cons> <tok cat="NN">secretion</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">anti-CD3</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
875731607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Comparison</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">dose</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">anti-CD3</tok></cons> <tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="JJ">half-maximal</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">achieved</tok> <cons cat="NP" ref="i4" null="NONE"></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NN">dose</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">anti-CD3</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="ADJP"><cons cat="NP"><tok cat="RB">freshly</tok> <tok cat="VBN">isolated</tok></cons></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="VBN">cultured</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
875731608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">dose</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">OKT3</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" null="NONE"></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">achieve</tok> <cons cat="NP"><tok cat="JJ">half-maximal</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><tok cat="DT">the</tok> <tok cat="JJ">same</tok></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PMA</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">concentrations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">alpha</tok> <tok cat="CD">4</tok> <tok cat="NN">beta</tok> <tok cat="CD">1</tok></cons> <tok cat="NNS">ligands</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
875731609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">costimulation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">alpha</tok> <tok cat="CD">4</tok> <tok cat="NN">beta</tok> <tok cat="CD">1</tok></cons> <tok cat="NNS">ligands</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">due</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stabilization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">substrate</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
875731610@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">rather</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">alpha</tok> <tok cat="CD">4</tok> <tok cat="NN">beta</tok> <tok cat="CD">1</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="RB">freshly</tok> <tok cat="VBN">isolated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">delivers</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">distinct</tok> <tok cat="NN">signal</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">synergizes</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">early</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">signals</tok></cons> <cons cat="VP"><tok cat="VBN">initiated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">TCR/CD3</tok></cons> <tok cat="NN">ligation</tok></cons></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">multiple</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">induction</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
875732601@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i27" role="SBJ"><cons cat="NP"><tok cat="JJ">Defective</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i27" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">impaired</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Fos</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">FosB</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">JunB</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">anergic</tok> <tok cat="NN">T</tok> <tok cat="NN">helper</tok> <tok cat="CD">1</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
875732602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i28" role="SBJ"><tok cat="JJ">Anergic</tok> <tok cat="JJ">CD4+</tok> <tok cat="NN">Th</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">do</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">produce</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons> <cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i30"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" ref="i28" id="i29" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">challenged</tok> <cons cat="NP" ref="i29" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">Ag-pulsed</tok> <tok cat="JJ">accessory</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">because</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">defect</tok></cons></cons><cons cat="ADVP" ref="i30" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
875732603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">work</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="JJ">anergic</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">defective</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">up-regulate</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">binding</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">transactivation</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">critical</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">DNA</tok> <tok cat="NN">enhancer</tok> <tok cat="NNS">elements</tok></cons><tok cat="COLON">:</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">NF-AT</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">sequence</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i31"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i31" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">heterotrimeric</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NFATp</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Fos</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Jun</tok></cons></cons> <tok cat="NN">protein</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Activator</tok> <tok cat="NN">Protein-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AP-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="LRB">(</tok><cons cat="SBAR"><cons cat="WHNP" id="i32"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i32" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Fos</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Jun</tok></cons></cons> <tok cat="NNS">heterodimers</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@69@@oe@16-12-2010
875732604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Western</tok> <tok cat="NN">blot</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i33" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">impaired</tok> <tok cat="NN">DNA-protein</tok> <tok cat="NNS">interactions</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">anergic</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i33" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">poor</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inducible</tok> <tok cat="NN">AP-1</tok> <tok cat="NN">family</tok> <tok cat="NNS">members</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Fos</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">FosB</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">JunB</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
875732605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">reduced</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">proteins</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">result</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">global</tok> <tok cat="JJ">TCR/CD3-signaling</tok> <tok cat="NN">defect</tok></cons></cons></cons> <cons cat="SBAR"><tok cat="IN">because</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">CD3</tok> <tok cat="NN">cross-linking</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">equivalent</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">intracellular-free</tok> <tok cat="NN">calcium</tok> <tok cat="NNS">ions</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NFATp</tok> <tok cat="NN">dephosphorylation</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">binding</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADJP"><tok cat="JJ">normal</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">anergic</tok></cons></cons> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@16-12-2010
875732606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i34" role="SBJ"><tok cat="JJ">defective</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="S"><cons cat="NP" ref="i34" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="PP" role="PRD"><tok cat="JJ">due</tok><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">part</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">selective</tok> <tok cat="NN">block</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">AP-1</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Fos</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Jun</tok></cons></cons> <tok cat="NN">family</tok> <tok cat="NNS">members</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">anergic</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
875760301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">CTL</tok> <tok cat="NN">recognition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">altered</tok> <tok cat="NN">peptide</tok></cons></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">asparagine</tok> <tok cat="NN">bond</tok> <tok cat="NN">rearrangement</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
875760302@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">Implications</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">immunity</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">vaccine</tok> <tok cat="NN">design</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@7@@oe@16-12-2010
875760303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i46" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">extent</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i47"><tok cat="TO">to</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i48" role="SBJ"><cons cat="NP"><tok cat="NNS">peptides</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><cons cat="ADJP"><cons cat="ADVP" syn="COOD"><cons cat="ADVP"><tok cat="RB">chemically</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">post-translationally</tok></cons></cons> <tok cat="VBN">modified</tok></cons> <tok cat="NN">amino</tok> <tok cat="NN">acid</tok> <tok cat="JJ">side</tok> <tok cat="NNS">chains</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">recognized</tok> <cons cat="NP" ref="i48" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">primed</tok> <tok cat="NN">CTL</tok></cons></cons> <cons cat="PP" ref="i47" null="T"></cons></cons></cons></cons></cons></cons><cons cat="VP"><tok cat="VBZ">has</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="ADVP"><tok cat="RB">clearly</tok></cons> <cons cat="VP"><tok cat="VBN">defined</tok><cons cat="NP" ref="i46" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
875760304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CTL</tok> <tok cat="NN">recognition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="JJ">I-restricted</tok></cons> <tok cat="NN">peptide</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cyclized</tok> <tok cat="NN">asparagine</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">succinimide</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">residue</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
875760305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">This</tok> <tok cat="NN">modification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">asparagine</tok> <tok cat="JJ">side</tok> <tok cat="NN">chain</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">common</tok> <tok cat="JJ">intermediate</tok> <tok cat="NN">structure</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">deamidation</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">isomerization</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">bond</tok> <tok cat="NNS">rearrangements</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">amide-containing</tok> <tok cat="NN">amino</tok> <tok cat="NNS">acids</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <tok cat="RB">also</tok> <cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">side</tok> <tok cat="NN">reaction</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">peptide</tok> <tok cat="NN">synthesis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
875760306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">CTL</tok></cons> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="VP"><tok cat="VBD">recognized</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">succinimide-containing</tok> <tok cat="NN">peptide</tok></cons> <cons cat="VP"><tok cat="VBG">showing</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">only</tok> <tok cat="JJ">weak</tok></cons> <tok cat="NN">cross-reactivity</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">concentrations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">parent</tok> <tok cat="NN">peptide</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="JJ">unmodified</tok> <tok cat="NN">asparagine</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
875760307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Similarly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">CTL</tok></cons> <cons cat="VP"><tok cat="VBN">raised</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">parent</tok> <tok cat="NN">peptide</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">recognize</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">succinimide</tok> <tok cat="NN">derivative</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">peptide</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
875760308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i49" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">Naturally</tok> <tok cat="VBN">processed</tok></cons> <tok cat="NNS">forms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">structures</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">likely</tok> <cons cat="S"><cons cat="NP" ref="i49" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">occur</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="VBN">given</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">importance</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">frequency</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">deamidation</tok></cons></cons> <cons cat="ADVP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
875760309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">since</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">succinimide</tok> <tok cat="NNS">intermediates</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">deamidated</tok> <tok cat="NNS">peptides</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">occasionally</tok></cons> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">very</tok> <tok cat="JJ">stable</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">peptides</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">potential</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">act</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">altered</tok> <tok cat="NNS">self-Ags</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">significant</tok> <tok cat="NNS">implications</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">autoimmunity</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
875760310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i50" role="SBJ"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">unwanted</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="RB">potentially</tok> <tok cat="JJ">hazardous</tok></cons></cons> <tok cat="NNS">specificities</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">elicited</tok> <cons cat="NP" ref="i50" null="NONE"></cons><cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i51"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="JJ">synthetic</tok> <tok cat="NNS">peptides</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">subunit</tok> <tok cat="NNS">vaccines</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i52"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">succinimide</tok> <tok cat="NNS">residues</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">form</tok> <cons cat="ADVP"><tok cat="RB">spontaneously</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">storage</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">chemical</tok> <tok cat="NN">synthesis</tok></cons></cons></cons><cons cat="PP" ref="i52" null="T"></cons></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i51" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
875889801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i53" role="SBJ"><tok cat="NN">C/EBP</tok> <tok cat="NNS">activators</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i53" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">replication</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">proviral</tok> <tok cat="NN">induction</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
875889802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Previous</tok> <tok cat="NN">work</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">C/EBP</tok> <tok cat="NNS">sites</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">C/EBP</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NNS">activators</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">necessary</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">LTR</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">monocytes/macrophages</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
875889803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i54"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">C/EBP</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBP">play</tok> <cons cat="NP" ref="i54" null="T"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">induction</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">replication</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
875889804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Ectopic</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">dominant</tok> <tok cat="JJ">negative</tok> <tok cat="NN">C/EBP</tok> <tok cat="NN">protein</tok> <tok cat="NN">LIP</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">virus</tok> <tok cat="NN">production</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">U1</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">demonstrating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i56" role="SBJ"><tok cat="NN">C/EBP</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i56" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">provirus</tok> <tok cat="NN">induction</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
875889805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">U1</tok> <tok cat="NNS">lines</tok></cons> <cons cat="VP"><tok cat="VBG">overexpressing</tok> <cons cat="NP"><tok cat="NN">C/EBP</tok> <tok cat="NN">activator</tok> <tok cat="NN">NF-IL-6</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">produced</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJR">more</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">virus</tok> <tok cat="NNS">particles</tok></cons></cons></cons> <cons cat="PP" ref="i101" null="ICH"></cons></cons><cons cat="PP" adv="TMP"><tok cat="VBG">following</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NN">activation</tok></cons></cons> <cons cat="PP" id="i101"><tok cat="IN">than</tok> <cons cat="NP"><tok cat="NN">control</tok> <tok cat="NNS">lines</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">demonstrating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP"><tok cat="NN">C/EBP</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="VBG">limiting</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">virus</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
875889806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">harboring</tok> <tok cat="NNS">mutations</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="CD">two</tok> <tok cat="NN">C/EBP</tok> <tok cat="NNS">sites</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="VBN">crippled</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">replicate</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">U937</tok> <tok cat="JJ">promonocytic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i60" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">sites</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i60" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">replication</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
875889807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">identify</tok> <cons cat="NP"><tok cat="NN">C/EBP</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">regulators</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">monocytes/macrophages</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
875972101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">Stat1</tok> <tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NN">cell</tok> <tok cat="NNS">cultures</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cyclic</tok> <tok cat="NN">AMP</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
875972102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cascade</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">subsequent</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">proliferation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="NN">effector</tok> <tok cat="NNS">phenotypes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
875972103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i38" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="VP"><tok cat="VBG">belonging</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">transducer</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">STAT</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">family</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP" ref="i38" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">PHA-activated</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">purified</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">cross-linking</tok> <tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <tok cat="NN">CD3</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
875972104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Cell</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="VBN">delayed</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">activity</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i39"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i39" id="i40" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="JJ">sustained</tok> <cons cat="NP" ref="i40" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NNS">days</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">composed</tok> <cons cat="NP" ref="i40" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">predominantly</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Stat1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Stat3</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i40" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">cycloheximide</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">actinomycin</tok> <tok cat="NN">D</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
875972105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i43" role="SBJ"><tok cat="VBN">Increased</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Stat1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Stat3</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok></cons></cons> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">detected</tok><cons cat="NP" ref="i43" null="NONE"></cons><tok cat="COMMA">,</tok> <cons cat="PP" adv="TMP"><cons cat="ADVP"><tok cat="RB">respectively</tok></cons> <cons cat="NP"><cons cat="QP"><tok cat="CD">4</tok> <tok cat="CC">and</tok> <tok cat="CD">24</tok></cons> <tok cat="NN">h</tok></cons> <tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">activation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
875972106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cAMP</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok> <tok cat="NN">pathway</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i44"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i44" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">skews</tok> <cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">toward</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">Th2</tok> <tok cat="NN">pattern</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">preferential</tok> <tok cat="NN">suppression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Stat1</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
875972107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">cAMP</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">receptor</tok> <tok cat="NNS">components</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-4</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha-chain</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CD69</tok></cons></cons> <tok cat="NN">expression</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">activator</tok> <tok cat="NN">protein</tok> <tok cat="CD">1</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
875972108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">cAMP</tok> <tok cat="NN">signaling</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="NN">Stat1</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="JJ">different</tok> <tok cat="NNS">levels</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">suppression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">binding</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">down-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Stat1</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">protein</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons></cons> <tok cat="NNS">levels</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
875972109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i45"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i45" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">regulates</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="JJ">functional</tok> <tok cat="NN">phenotype</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">distinct</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cytokine-activated</tok> <tok cat="NN">Janus</tok> <tok cat="NN">kinase-STAT</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
875973301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">lymphocyte</tok></cons> <tok cat="NN">activation</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">cAMP</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">down-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">parallel</tok></cons> <cons cat="ADJP"><tok cat="JJ">mitogen-activated</tok></cons></cons> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons> <cons cat="NP" ref="i21" null="ICH"></cons></cons><tok cat="NNS">pathways</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i21"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">extracellular</tok> <tok cat="JJ">signal-related</tok> <tok cat="NN">kinase</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok> <tok cat="JJ">N-terminal</tok> <tok cat="NN">kinase</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@28@@oe@16-12-2010
875973302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">proliferation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">signals</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TCR</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">co-receptor</tok> <tok cat="NN">molecule</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">CD28</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">activate</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">parallel</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="RB">partially</tok> <tok cat="JJ">cross-reactive</tok></cons></cons> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@29@@oe@16-12-2010
875973303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">disrupted</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">agonists</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">utilize</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">adenylate</tok> <tok cat="NN">cyclase</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cAMP-dependent</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">A</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PKA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
875973304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">adenylate</tok> <tok cat="NN">cyclase</tok></cons> <tok cat="NN">activator</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">forskolin</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">anti-CD3-induced</tok> <tok cat="NN">shift</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Lck</tok></cons> <tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">intervention</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">TCR-coupled</tok> <tok cat="JJ">phosphoinositide</tok> <tok cat="NN">turnover</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">precedes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PKC</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
875973305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">shift</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Lck</tok></cons></cons> <cons cat="VP"><tok cat="VBG">following</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">direct</tok> <cons cat="NP"><tok cat="NN">PKC</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">12-O-tetradecanoyl</tok> <tok cat="NN">phorbol</tok> <tok cat="NN">13-acetate</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">bypasses</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">early</tok></cons> <cons cat="ADJP"><tok cat="JJ">receptor-triggered</tok></cons> <cons cat="ADJP"><tok cat="JJ">biochemical</tok></cons></cons> <tok cat="NNS">events</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">insensitive</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">forskolin</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
875973306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Nevertheless</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">forskolin</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">PKC</tok></cons> <tok cat="JJ">downstream</tok> <tok cat="NNS">events</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">c-jun</tok></cons> <tok cat="NN">expression</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">critical</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok> <tok cat="NN">process</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
875973307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="ADVP"><tok cat="RB">further</tok></cons> <cons cat="VP"><tok cat="VB">analyze</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">potential</tok> <tok cat="NN">cross</tok> <tok cat="NNS">points</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADVP"><tok cat="RB">positively</tok></cons> <cons cat="NP" ref="i9" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADVP"><tok cat="RB">negatively</tok></cons> <cons cat="NP" ref="i9" null="RNR"></cons></cons><cons cat="NP" id="i9"><tok cat="VBG">regulating</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">tested</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">activators</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">adenylate</tok> <tok cat="NN">cyclase</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">PKA</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">parallel</tok></cons> <cons cat="ADJP"><tok cat="JJ">mitogen-activated</tok></cons></cons> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="JJ">extracellular</tok> <tok cat="JJ">signal-regulated</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ERK</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok> <tok cat="JJ">N-terminal</tok> <tok cat="NN">kinase</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@50@@oe@16-12-2010
875973308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">PKC-specific</tok> <tok cat="NN">inhibitor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">GF109203X</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">PKC-depleted</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">large</tok> <tok cat="NN">part</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">anti-CD3-induced</tok> <cons cat="NP"><tok cat="NN">ERK</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><cons cat="NP"><tok cat="NN">PKC</tok></cons> <tok cat="JJ">dependent</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
875973309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="CC">Both</tok> <cons cat="NP"><tok cat="JJ">PKC- dependent</tok> <cons cat="NP" ref="i11" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><tok cat="JJ">-independent</tok> <cons cat="NP" ref="i11" null="RNR"></cons></cons><cons cat="NP" id="i11"><tok cat="NN">activation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ERK</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">sensitive</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">forskolin</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">cell-permeable</tok> <tok cat="NN">cAMP</tok> <tok cat="NN">analogue</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">dbcAMP</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
875973310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">12-O-tetradecanoyl</tok> <tok cat="NN">phorbol</tok> <tok cat="NN">13-acetate</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">ionomycin</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i12"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i12" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">synergized</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="ADVP"><tok cat="RB">fully</tok></cons> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok> <tok cat="JJ">N-terminal</tok> <tok cat="NN">kinase</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">sensitive</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">forskolin</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
875973311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i13" role="SBJ"><tok cat="NN">PKA</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i13" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">interfering</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">multiple</tok> <tok cat="NNS">events</tok></cons> <cons cat="PP"><tok cat="IN">along</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="VP"><tok cat="VBG">operating</tok> <cons cat="ADVP"><tok cat="RB">downstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TCR</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CD28</tok> <tok cat="NN">co-receptor</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
876030901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">Abnormalities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p16</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p15</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CDK4</tok></cons></cons> <tok cat="NNS">genes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">recurrent</tok> <tok cat="JJ">malignant</tok> <tok cat="NNS">astrocytomas</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
876030902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i38" role="SBJ"><cons cat="NP"><tok cat="NNS">Abnormalities</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p16</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p15</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CDK4</tok></cons></cons> <tok cat="NNS">genes</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i39"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i39" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">regulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transition</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">G1</tok> <tok cat="NN">phase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP" ref="i38" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">malignant</tok> <tok cat="NN">progression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">astrocytomas</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
876030903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">dysfunction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">recurrence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">glial</tok> <tok cat="NNS">tumors</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i40"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i40" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">highly</tok> <tok cat="JJ">malignant</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="JJ">first</tok> <tok cat="NN">presentation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
876030904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">10</tok> <tok cat="VBN">matched</tok> <tok cat="NNS">pairs</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NN">grade</tok> <tok cat="JJ">malignant</tok> <tok cat="NNS">astrocytomas</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">subsequent</tok> <tok cat="NNS">recurrences</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><tok cat="CD">three</tok> <tok cat="JJ">distinct</tok> <tok cat="NNS">groups</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
876030905@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">primary</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">recurrent</tok></cons></cons> <tok cat="NNS">tumors</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Group</tok> <tok cat="NN">A</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">show</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">structural</tok> <tok cat="NNS">alterations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p16</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p15</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">CDK4</tok></cons></cons> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" id="i41" role="SBJ"><cons cat="NP"><tok cat="JJ">homozygous</tok> <tok cat="NN">codeletion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p16</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p15</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i41" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADJP"><tok cat="JJ">primary</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">recurrent</tok></cons></cons> <tok cat="NNS">tumors</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Group</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
876030906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">primary</tok> <tok cat="NNS">tumors</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Group</tok> <tok cat="NN">C</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">normal</tok> <tok cat="NN">profile</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p16</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p15</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CDK4</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="NN">presentation</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
876030907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">Upon</tok> <cons cat="NP"><tok cat="NN">recurrence</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">tumors</tok></cons> <cons cat="VP"><tok cat="VBD">sustained</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">deletion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">p16</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">codeletion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">p16</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p15</tok></cons></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">amplification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CDK4</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
876030908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NNS">differences</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">anaplastic</tok> <tok cat="NNS">astrocytomas/glioblastomas</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">subsequent</tok> <tok cat="NNS">recurrences</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i42"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i42" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">clinically</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">indistinguishable</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJR">better</tok> <tok cat="JJ">therapeutic</tok> <tok cat="NNS">options</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">treatment</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
876138101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Transcriptional</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">EBV-positive</tok> <tok cat="JJ">gastric</tok> <tok cat="NN">carcinoma</tok></cons></cons></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">unique</tok> <tok cat="JJ">viral</tok> <tok cat="NN">latency</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">tumour</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@16-12-2010
876138102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">case-oriented</tok> <tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">association</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EBV</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">gastric</tok> <tok cat="NN">carcinoma</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBG">accumulating</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">interaction</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">EBV</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">gastric</tok> <tok cat="NN">epithelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">is/are</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">largely</tok> <tok cat="JJ">unknown</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
876138103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><tok cat="CD">seven</tok> <tok cat="JJ">EBV-positive</tok> <tok cat="JJ">gastric</tok> <tok cat="NN">carcinoma</tok> <tok cat="NNS">tissues</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">level</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHPP" id="i61"><tok cat="IN">from</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">studies</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">EBV</tok> <tok cat="NN">oncogenicity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">epithelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">will</tok> <cons cat="VP"><tok cat="VB">benefit</tok><cons cat="PP" ref="i61" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
876138104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Reverse</tok> <tok cat="NN">transcription-PCR</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="QP"><tok cat="DT">all</tok> <tok cat="CD">seven</tok></cons> <tok cat="JJ">EBV-positive</tok> <tok cat="NN">tumour</tok> <tok cat="NNS">tissues</tok></cons> <cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">EBV</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">antigen</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EBNA</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CD">1</tok> <tok cat="NN">mRNA</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">EBNA2</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
876138105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i62" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">EBNA</tok> <tok cat="NN">transcription</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">initiated</tok> <cons cat="NP" ref="i62" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">three</tok> <tok cat="NN">EBNA</tok> <tok cat="NNS">promoters</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Qp</tok></cons></cons></cons></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i63" role="SBJ"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">Cp</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Wp</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">silent</tok></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" ref="i63" role="SBJ" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">lack</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">EBNA2</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
876138106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i64" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Latent</tok> <tok cat="NN">membrane</tok> <tok cat="NN">protein</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LMP</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">2A</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i64" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">seven</tok> <tok cat="NNS">cases</tok></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok></cons> <cons cat="S"><cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i65" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="CC">neither</tok> <cons cat="NP"><tok cat="NN">LMP1</tok></cons> <tok cat="CC">nor</tok> <cons cat="NP"><tok cat="NN">LMP2B</tok></cons></cons> <tok cat="NN">mRNA</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i65" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">any</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">tumours</tok></cons> <cons cat="VP"><tok cat="VBN">tested</tok><cons cat="NP" null="NONE"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
876138107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Transcripts</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">BamHI-A</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">viral</tok> <tok cat="NN">genome</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">detectable</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">all</tok> <tok cat="NNS">cases</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
876138108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i66" role="SBJ"><cons cat="NP"><tok cat="NN">BZLF1</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">product</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">immediate-early</tok> <tok cat="NN">gene</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">EBV</tok> <tok cat="NN">replication</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i66" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">any</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRP">them</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">thereby</tok></cons> <cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">tumour</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">carried</tok> <cons cat="NP"><tok cat="NN">EBV</tok> <tok cat="NNS">genomes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">tightly</tok> <tok cat="JJ">latent</tok></cons> <tok cat="NN">form</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
876138109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><cons cat="ADVP"><tok cat="RB">further</tok></cons> <tok cat="VBD">extended</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">our</tok> <tok cat="JJ">previous</tok> <tok cat="NNS">data</tok></cons> <cons cat="PP"><tok cat="VBG">regarding</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">EBV</tok> <tok cat="NN">latency</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">gastric</tok> <tok cat="NN">carcinoma</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">protein</tok> <tok cat="NN">level</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">affirmed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">programme</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">tumour</tok></cons></cons></cons></cons></cons> <cons cat="VP"><cons cat="ADVP"><cons cat="ADVP"><tok cat="RBR">more</tok> <tok cat="RB">closely</tok></cons> <cons cat="PP" ref="i67" null="ICH"></cons></cons><tok cat="VBZ">resembles</tok> <cons cat="NP"><tok cat="LQT">'</tok><cons cat="NP"><tok cat="NN">latency</tok> <tok cat="CD">I</tok></cons><tok cat="RQT">'</tok> <cons cat="VP"><tok cat="VBN">represented</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">Burkitt</tok><tok cat="POS">'s</tok></cons> <tok cat="NN">lymphoma</tok></cons></cons></cons></cons> <cons cat="PP" id="i67"><tok cat="IN">than</tok> <cons cat="NP"><tok cat="LQT">'</tok><cons cat="NP"><tok cat="NN">latency</tok> <tok cat="CD">II</tok></cons><tok cat="RQT">'</tok> <cons cat="VP"><tok cat="VBN">represented</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">majority</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nasopharyngeal</tok> <tok cat="NNS">carcinomas</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@57@@oe@16-12-2010
876402701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Chronic</tok> <tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">disrupts</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">autoregulatory</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappaB/Rel</tok> <tok cat="NN">pathway</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="VBN">enhanced</tok> <tok cat="NN">IkappaBalpha</tok> <tok cat="NN">degradation</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
876402702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Productive</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HIV-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">infection</tok></cons> <cons cat="VP"><tok cat="VBZ">causes</tok> <cons cat="NP"><tok cat="JJ">sustained</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">chronically</tok> <tok cat="VBN">infected</tok></cons> <tok cat="JJ">monocytic</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
876402703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">A</tok> <tok cat="JJ">direct</tok> <tok cat="JJ">temporal</tok> <tok cat="NN">correlation</tok></cons> <cons cat="VP"><tok cat="VBZ">exists</tok> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">infection</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">appearance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">myelomonoblastic</tok> <tok cat="NN">PLB-985</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
876402704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" ref="i52" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">examine</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">basis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">constitutive</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">HIV1 -infected</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i52" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">analyzed</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">turnover</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IkappaBalpha</tok> <tok cat="NN">protein</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">double-stranded</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">RNA-dependent</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PKR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">intracellular</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NNS">subunits</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PLB-985</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">U937</tok></cons></cons> <tok cat="JJ">myeloid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">models</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@16-12-2010
876402705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HIV-1</tok> <tok cat="NN">infection</tok></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">constitutive</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">low-level</tok></cons></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">1</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interferon</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IFN</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">level</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
876402706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i53" role="SBJ"><tok cat="JJ">Constitutive</tok> <tok cat="NN">PKR</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i53" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">HIV-1-infected</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">result</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">low-level</tok> <tok cat="NN">IFN</tok> <tok cat="NN">production</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">since</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">anti-IFN-alpha/beta</tok> <tok cat="NN">antibody</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">decreased</tok> <cons cat="NP"><tok cat="NN">PKR</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
876402707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IkappaBalpha</tok> <tok cat="NN">turnover</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="VBN">increased</tok> <tok cat="NN">degradation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IkappaBalpha</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">HIV-1-infected</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i54"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i54" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">account</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">constitutive</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
876402708@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" syn="COOD" id="i55" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">dramatic</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">intracellular</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NNS">subunits</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Rel</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-kappaB2</tok> <tok cat="NN">p100</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">moderate</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappaB2</tok> <tok cat="NN">p52</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">RelA(p65)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i55" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">HIV-1-infected</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" id="i56" role="SBJ"><tok cat="NN">NF-kappaB1</tok> <tok cat="NN">p105/p50</tok> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">altered</tok> <cons cat="NP" ref="i56" null="NONE"></cons><cons cat="PP"><tok cat="JJ">relative</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">uninfected</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
876402709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IFN</tok> <tok cat="NN">production</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">PKR</tok> <tok cat="NN">activity</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i57"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">turn</tok></cons></cons> <cons cat="NP" ref="i57" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">enhanced</tok> <tok cat="NN">IkappaBalpha</tok> <tok cat="NN">phosphorylation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">subsequent</tok> <tok cat="NN">degradation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
876402710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Nuclear</tok> <tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NNS">subunits</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="ADVP"><tok cat="RB">ultimately</tok></cons> <cons cat="VP"><tok cat="VB">increase</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">intracellular</tok> <tok cat="NN">pool</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB/IkappaBalpha</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">autoregulatory</tok> <tok cat="NN">mechanism</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
876402711@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="VBN">Enhanced</tok> <tok cat="NN">turnover</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IkappaBalpha</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">accumulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB/Rel</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">chronically</tok> <tok cat="JJ">activated</tok></cons> <tok cat="NN">state</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">HIV-1-infected</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
876965001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Sequence-specific</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">B-cell-specific</tok> <tok cat="NN">coactivator</tok> <tok cat="NN">OCA-B</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
876965002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i36" role="SBJ"><cons cat="NP"><tok cat="JJ">B-cell-specific</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">immunoglobulin</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i36" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">POU</tok> <tok cat="NN">domain</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Oct-1</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">Oct-2</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">B-cell-specific</tok> <tok cat="NN">coactivator</tok> <tok cat="NN">OCA-B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Bob-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">OBF-1</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">prototype</tok> <tok cat="NN">octamer</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
876965003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">find</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i37" role="SBJ"><tok cat="NN">OCA-B</tok></cons> <cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="NP"><tok cat="NN">DNA</tok></cons> <cons cat="ADVP"><tok cat="RB">directly</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">major</tok> <tok cat="NN">groove</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="NNS">subdomains</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">POU</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" ref="i37" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">requiring</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">A</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">fifth</tok> <tok cat="NN">position</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">octamer</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">contact</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">POU</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
876965004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">An</tok> <tok cat="JJ">amino-terminal</tok> <tok cat="NN">fragment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">OCA-B</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">octamer</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">absence</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">POU</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NN">sequence</tok> <tok cat="NN">specificity</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
876965005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i38" role="SBJ"><tok cat="NN">Coactivator</tok> <tok cat="NN">OCA-B</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">undergo</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">POU-dependent</tok> <tok cat="JJ">conformational</tok> <tok cat="NN">change</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i39"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i39" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">exposes</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">amino</tok> <tok cat="NN">terminus</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" ref="i38" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">allowing</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">recognize</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">DNA</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">major</tok> <tok cat="NN">groove</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBG">binding</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Oct-1</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">Oct-2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
876965006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">recognition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">POU</tok> <tok cat="NN">domain</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">octamer</tok> <tok cat="NN">sequence</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">OCA-B</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">provides</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">differential</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">octamer</tok> <tok cat="NNS">sites</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="NNS">genes</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">ubiquitous</tok> <tok cat="NN">factor</tok> <tok cat="NN">Oct-1</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010