937895301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="JJ">Nuclear</tok> <tok cat="NN">localization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">RelB</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">effective</tok></cons> <cons cat="ADJP"><tok cat="JJ">antigen-presenting</tok></cons></cons> <tok cat="NN">cell</tok> <tok cat="NN">function</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@12@@oe@16-12-2010
937895302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Dendritic</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">DC</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">potent</tok> <cons cat="NP"><tok cat="NNS">APCs</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">enter</tok> <cons cat="NP"><tok cat="VBG">resting</tok> <tok cat="NNS">tissues</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="NNS">precursors</tok></cons></cons></cons> <tok cat="CC">and</tok><tok cat="COMMA">,</tok> <cons cat="VP" syn="COOD"><cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">Ag</tok> <tok cat="NN">exposure</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBP">differentiate</tok></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">migrate</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="VBG">draining</tok> <tok cat="NN">lymph</tok> <tok cat="NNS">nodes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@28@@oe@16-12-2010
937895303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">phenotype</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">RelB</tok></cons> <tok cat="NN">knockout</tok> <tok cat="NNS">mice</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">implicates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">member</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B/Rel</tok> <tok cat="NN">family</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">DC</tok></cons> <tok cat="NN">differentiation</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
937895304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="ADVP"><tok cat="RB">further</tok></cons> <cons cat="VP"><tok cat="VB">elucidate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">RelB</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">DC</tok></cons> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">mRNA</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">protein</tok> <tok cat="NN">expression</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">RelB</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">immature</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="VBN">differentiated</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="JJ">human</tok> <tok cat="NNS">DC</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><tok cat="JJ">other</tok> <cons cat="NP"><tok cat="NN">PB</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NN">cell</tok> <tok cat="NNS">populations</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
937895305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">RelB</tok></cons> <tok cat="NN">protein</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">mRNA</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NNS">monocytes</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="VBN">differentiated</tok> <tok cat="NNS">DC</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">monocyte-derived</tok> <tok cat="NNS">DC</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
937895306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Immunohistochemical</tok> <tok cat="NN">staining</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="NP"><tok cat="NN">RelB</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="VBN">differentiated</tok> <tok cat="NN">lymph</tok> <tok cat="NN">node</tok> <tok cat="NN">interdigitating</tok> <tok cat="NNS">DC</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">follicular</tok> <tok cat="NNS">DC</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">undifferentiated</tok> <tok cat="NNS">DC</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">skin</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
937895307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="ADJP"><tok cat="JJ">Active</tok> <tok cat="JJ">nuclear</tok></cons> <cons cat="NP"><tok cat="NN">RelB</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">supershift</tok> <tok cat="NN">assay</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">differentiated</tok> <cons cat="NP"><tok cat="NNS">DC</tok></cons></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="NN">PB</tok> <tok cat="NNS">precursors</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
937895308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <cons cat="NP"><tok cat="JJ">RelB+</tok> <tok cat="NN">APC</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">potent</tok> <tok cat="NNS">stimulators</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">MLR</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
937895309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="NN">RelB</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="ADVP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADVP"><tok cat="RB">transcriptionally</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">post-translationally</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
937895310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Within</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">RelB</tok></cons> <tok cat="NN">may</tok></cons> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="VP"><tok cat="VB">transactivate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">genes</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">critical</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">APC</tok></cons> <tok cat="NN">function</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
937900201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">inhibitor</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B-alpha/MAD-3</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">Mycobacterium</tok> <tok cat="NN">tuberculosis</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">tuberculosis</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@16-12-2010
937900202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">Blood</tok> <tok cat="NNS">monocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">active</tok> <tok cat="NN">tuberculosis</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">evidenced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">stimulated</tok> <tok cat="NN">release</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">proinflammatory</tok> <tok cat="NNS">cytokines</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">spontaneous</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@36@@oe@16-12-2010
937900203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Further</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NNS">patients</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">augmented</tok> <tok cat="NN">susceptibility</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">productive</tok> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok></cons></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
937900204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Mycobacterium</tok> <tok cat="NN">tuberculosis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NNS">components</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">strong</tok> <tok cat="NNS">signals</tok></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i9" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons></cons></cons><cons cat="ADVP" ref="i9" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
937900205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">basis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">active</tok> <tok cat="NN">tuberculosis</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">M.</tok> <tok cat="NN">tuberculosis</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
937900206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">constitutive</tok> <tok cat="NN">degradation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B-alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">major</tok> <cons cat="NP"><tok cat="JJ">cytoplasmic</tok> <tok cat="NN">inhibitor</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="ADJP"><tok cat="RB">freshly</tok> <tok cat="VBN">isolated</tok></cons> <cons cat="NP"><tok cat="NN">PBMC</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">tuberculosis</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
937900207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B-alpha</tok></cons> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PBMC</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">subjects</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">nontuberculous</tok> <tok cat="JJ">pulmonary</tok></cons> <tok cat="NNS">conditions</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">intact</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
937900208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Further</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">tuberculous</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
937900209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B-alpha</tok></cons> <tok cat="NN">gene</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i14"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i14" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">responsive</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">up-regulated</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PBMC</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NNS">patients</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">subjects</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">nontuberculous</tok> <tok cat="NN">lung</tok> <tok cat="NNS">diseases</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
937900210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <cons cat="NP"><tok cat="JJ">adherence-associated</tok> <tok cat="JJ">early</tok> <tok cat="NNS">genes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-8</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="NN">beta</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">up-regulated</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PBMC</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">tuberculous</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
937900211@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Further</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">M.</tok> <tok cat="NN">tuberculosis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <cons cat="NP"><tok cat="NN">tuberculin</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="VBN">purified</tok> <tok cat="NN">protein</tok> <tok cat="NN">derivative</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">degradation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B-alpha</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B-alpha</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="VBN">purified</tok> <tok cat="NN">protein</tok> <tok cat="NN">derivative</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
938382801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Pathogenesis</tok><tok cat="PERIOD">.</tok></cons></cons>@@@@1@2@@oe@16-12-2010
938382802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">There</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">many</tok> <tok cat="NNS">hypotheses</tok></cons> <cons cat="VP"><tok cat="VBG">concerning</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathogenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">endometriosis</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">though</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">no</tok> <tok cat="JJ">single</tok> <tok cat="NN">theory</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">explain</tok> <cons cat="NP"><tok cat="DT">all</tok> <tok cat="NNS">cases</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
938382803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">likely</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="JJ">several</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">involved</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
938382804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Early</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBD">concentrated</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">histogenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">endometriotic</tok> <tok cat="NN">lesion</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
938382805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Recent</tok> <tok cat="NN">evidence</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">components</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immune</tok> <tok cat="NN">system</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathogenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">endometriosis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
938382806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">review</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">considers</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">theories</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">histogenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">endometriosis</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">discusses</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">possible</tok> <tok cat="JJ">immune</tok> <tok cat="NNS">factors</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" id="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathophysiology</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">disease</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
938466101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-fos</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">correlates</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">IFN-alpha</tok></cons> <tok cat="NN">responsiveness</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Philadelphia</tok> <tok cat="NN">chromosome</tok> <tok cat="JJ">positive</tok> <tok cat="JJ">chronic</tok> <tok cat="JJ">myelogenous</tok> <tok cat="NN">leukemia</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
938466102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBZ">evaluates</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">i</tok><tok cat="RRB">)</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">constitutive</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">oncogene</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p53</tok> <tok cat="NNS">transcripts</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">chronic</tok> <tok cat="NN">phase</tok> <tok cat="NN">CML</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">ii</tok><tok cat="RRB">)</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NNS">modulations</tok></cons> <cons cat="ADJP"><tok cat="JJ">subsequent</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <tok cat="NN">therapy</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">rIFN-alpha</tok> <tok cat="NN">2c</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@33@@oe@16-12-2010
938466103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">pbmc</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">bone</tok> <tok cat="NN">marrow</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">26</tok> <tok cat="NNS">patients</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-fos</tok><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">c-myc</tok><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p53</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">hybrid</tok> <tok cat="NN">bcr/abl</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">mRNA</tok> <tok cat="NNS">levels</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
938466104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">Results</tok></cons> <cons cat="VP"><tok cat="VBD">indicated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">i</tok><tok cat="RRB">)</tok></cons> <cons cat="NP" id="i8" role="SBJ"><tok cat="JJ">constitutive</tok> <cons cat="NP"><tok cat="NN">c-fos</tok> <tok cat="NN">transcript</tok></cons> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="UCP" syn="COOD"><cons cat="ADJP" role="PRD"><tok cat="RB">significantly</tok> <tok cat="JJR">higher</tok> <cons cat="PRN" ref="i30" null="ICH"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">subsequently</tok></cons> <tok cat="VBG">responding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IFN-alpha</tok></cons> <tok cat="NN">therapy</tok></cons></cons></cons></cons></cons></cons> <cons cat="PRN" id="i30"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">p</tok></cons> <cons cat="ADJP"><tok cat="JJR">&lt;</tok> <cons cat="NP"><tok cat="CD">0.01</tok></cons></cons></cons><tok cat="RRB">)</tok></cons> <tok cat="CC">and</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><cons cat="ADVP" id="i35"><tok cat="RB">positively</tok> <cons cat="PRN" ref="i31" null="ICH"></cons></cons><tok cat="VBN">correlated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP" id="i36"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">proportion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons> <cons cat="PRN" id="i31"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">r</tok></cons> <cons cat="ADJP"><tok cat="JJ">=</tok> <cons cat="NP"><tok cat="CD">0.6895</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">p</tok></cons> <cons cat="ADJP"><tok cat="JJR">&lt;</tok> <cons cat="NP"><tok cat="CD">0.01</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons map="i35" cat="ADVP"><tok cat="RB">negatively</tok> <cons cat="PRN" ref="i32" null="ICH"></cons></cons><cons map="i36" cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">proportion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">immature</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP" ref="i37" null="ICH"></cons></cons></cons></cons></cons><cons cat="PRN" id="i32"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">r</tok></cons> <cons cat="ADJP"><tok cat="JJ">=</tok> <cons cat="NP"><tok cat="CD">-0.568</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">p</tok></cons> <cons cat="ADJP"><tok cat="JJR">&lt;</tok> <cons cat="NP"><tok cat="CD">0.01</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons> <cons cat="VP" id="i37"><tok cat="VBN">contained</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">pbmc</tok></cons> <tok cat="NNS">preparations</tok></cons> <cons cat="VP"><tok cat="VBN">tested</tok><cons cat="NP" null="NONE"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">ii</tok><tok cat="RRB">)</tok></cons> <cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">constitutive</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">hybrid</tok> <tok cat="NN">bcr/abl</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">c-myc</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p53</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">positively</tok></cons> <cons cat="VP"><tok cat="VBN">correlated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">each</tok> <tok cat="JJ">other</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">failed</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">relate</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">disease</tok> <tok cat="NNS">parameters</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">iii</tok><tok cat="RRB">)</tok></cons> <cons cat="NP" id="i10" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">acute</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">chronic</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <tok cat="NN">exposure</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IFN-alpha</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">accompanied</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">upregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-fos</tok></cons> <cons cat="NP" null="QSTN"></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">downregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-myc</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="NNS">levels</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">responder</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@123@@oe@16-12-2010
938762301@GENIA Treebank@formal@@1@S@<cons cat="NP"><tok cat="LRB">[</tok><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">value</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">clinical</tok> <tok cat="NN">test</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">leukocytes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">chronic</tok> <tok cat="JJ">pulmonary</tok> <tok cat="NN">heart</tok> <tok cat="NN">disease</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="RRB">]</tok></cons>@@@@1@22@@oe@16-12-2010
938762302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">In</tok> <tok cat="NN">order</tok> <tok cat="TO">to</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VB">inquire</tok> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">functional</tok> <tok cat="NN">state</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">adrenal</tok> <tok cat="NN">cortex</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">pulmonary</tok> <tok cat="NN">heart</tok> <tok cat="NN">disease</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i27" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GCR</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">leukocytes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">chronic</tok> <tok cat="JJ">pulmonary</tok> <tok cat="NN">heart</tok> <tok cat="NN">disease</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">determined</tok> <cons cat="NP" ref="i27" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">radioligand-binding</tok> <tok cat="NN">assay</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i28" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">corresponding</tok> <tok cat="NN">plasma</tok> <tok cat="NN">cortisol</tok> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">assessed</tok> <cons cat="NP" ref="i28" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">radioimmune</tok> <tok cat="NNS">assays</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@54@@oe@16-12-2010
938762303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i29" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GCR</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP"><tok cat="VBN">reduced</tok> <cons cat="NP" ref="i29" null="NONE"></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons> <cons cat="NP" role="PRD"><cons cat="QP"><tok cat="JJR">&lt;</tok> <tok cat="CD">0.01</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i30" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">increased</tok> <cons cat="NP" ref="i30" null="NONE"></cons><cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i31"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i32" role="SBJ"><tok cat="PRPP">their</tok> <tok cat="NN">health</tok> <tok cat="NN">state</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">improved</tok><cons cat="NP" ref="i32" null="NONE"></cons><cons cat="ADVP" ref="i31" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
938762304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">still</tok></cons> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="JJR">lower</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">subjects</tok></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons> <cons cat="NP" role="PRD"><cons cat="QP"><tok cat="JJR">&lt;</tok> <tok cat="CD">0.01</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
938762305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i33" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GCR</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">greatly</tok></cons> <cons cat="VP"><tok cat="VBN">increased</tok> <cons cat="NP" ref="i33" null="NONE"></cons><cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i34"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i35" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">patients</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" ref="i35" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">oxygen</tok></cons></cons> <cons cat="ADVP" ref="i34" null="T"></cons></cons></cons></cons></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons> <cons cat="NP" role="PRD"><cons cat="QP"><tok cat="JJR">&lt;</tok> <tok cat="CD">0.01</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
938762306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i36" role="SBJ"><cons cat="NP"><tok cat="DT">No</tok> <tok cat="NN">difference</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">plasma</tok> <tok cat="NN">cortisol</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" ref="i36" null="NONE"></cons><cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">patients</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">healthy</tok> <tok cat="NNS">subjects</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons> <cons cat="NP" role="PRD"><cons cat="QP"><tok cat="JJR">&gt;</tok> <tok cat="CD">0.05</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
938762307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i37" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">adrenal</tok> <tok cat="NN">cortex</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">improved</tok> <cons cat="NP" ref="i37" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">compensation</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJR">lower</tok> <tok cat="NN">GCR</tok> <tok cat="NN">number</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">result</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lacking</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">oxygen</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
938762308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i38" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GCR</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">improved</tok> <cons cat="NP" ref="i38" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">inhalation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">oxygen</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
938762309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons> <cons cat="NP" role="SBJ"><tok cat="NN">oxygen</tok> <tok cat="NN">therapy</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">helpful</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBG">raising</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBG">controlling</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">development</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">disease</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
938847501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">Molecular</tok> <tok cat="NN">cloning</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="NN">characterization</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">cDNA</tok> <tok cat="NN">encoding</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="NP"><tok cat="NN">NFATc</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
938847502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="NN">Transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NFAT</tok> <tok cat="LRB">(</tok><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="IN">of</tok> <tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok><tok cat="RRB">)</tok> <tok cat="NN">family</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">play</tok> <cons cat="NP"><tok cat="JJ">important</tok> <tok cat="NNS">roles</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">immune</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">inflammatory</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">responses</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">cytokines</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">immunoregulatory</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@34@@oe@16-12-2010
938847503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">describe</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">cloning</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">characterization</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">full-length</tok> <cons cat="NP"><tok cat="NN">cDNA</tok> <tok cat="NN">encoding</tok> <tok cat="JJ">murine</tok> <tok cat="LRB">(</tok><tok cat="JJ">m</tok><tok cat="RRB">)</tok> <tok cat="NN">NFATc</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">predicts</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><cons cat="NP"><tok cat="PDT">all</tok> <tok cat="DT">the</tok> <cons cat="NP"><tok cat="VBN">conserved</tok> <tok cat="JJ">structural</tok> <tok cat="NNS">motifs</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFAT</tok> <tok cat="NN">family</tok> <tok cat="NNS">members</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">rel</tok> <tok cat="NN">homology</tok> <tok cat="NN">domain</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NFAT</tok> <tok cat="NN">homology</tok> <tok cat="NN">domain</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">translocation</tok> <tok cat="NNS">signals</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@16-12-2010
938847504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">mNFATc</tok></cons> <cons cat="VP"><tok cat="VBN">complexed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">bound</tok> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">NFAT</tok> <tok cat="NN">recognition</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">activated</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">co-transfected</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">COS-7</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
938847505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Northern</tok> <tok cat="NN">blot</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">cDNA</tok> <tok cat="NN">probe</tok></cons></cons> <cons cat="VP"><tok cat="VBD">hybridized</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="CD">4.5</tok> <tok cat="NN">kb</tok></cons> <tok cat="NN">transcript</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">highly</tok> <tok cat="JJ">inducible</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
938847506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">Northern</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">situ</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">hybridization</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><tok cat="NN">mNFATc</tok> <tok cat="NN">transcript</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">early</tok> <tok cat="NN">stage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">development</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
938847507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mouse</tok> <tok cat="NN">embryo</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><tok cat="NN">mNFATc</tok> <tok cat="NN">transcript</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP" id="i30"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP" id="i31"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">thymus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">lung</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">submandibular</tok> <tok cat="NN">gland</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons map="i30" cat="ADVP"><tok cat="RB">weakly</tok></cons> <cons map="i31" cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">skeletal</tok> <tok cat="NN">muscle</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">heart</tok></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">mNFATc</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP" syn="COOD"><tok cat="CC">both</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">embryogenesis</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
939069101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Phosphatidylinositol</tok> <tok cat="NN">3-kinase</tok></cons> <cons cat="VP"><tok cat="VBZ">couples</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">interleukin-2</tok> <tok cat="NN">receptor</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">cycle</tok> <tok cat="NN">regulator</tok></cons></cons> <cons cat="NP"><tok cat="NN">E2F</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
939069102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Cell</tok> <tok cat="NN">cycle</tok> <tok cat="NN">progression</tok></cons> <cons cat="VP"><tok cat="VBN">initiated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">interleukin-2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-2</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">critical</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">lymphoproliferation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@16-12-2010
939069103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">Phosphatidyl</tok> <tok cat="NN">inositol</tok> <tok cat="NN">3-kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PI3K</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">IL-2</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
939069104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">targets</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">PI3K</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">known</tok><cons cat="NP" ref="i6" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
939069105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">identify</tok> <cons cat="NP" id="i30"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">cycle</tok> <tok cat="NN">regulator</tok></cons> <cons cat="NP"><tok cat="NN">E2F</tok></cons></cons> <cons cat="PP" id="i31"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="NN">target</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons map="i30" cat="NP"><tok cat="NN">PI3K</tok></cons> <cons map="i31" cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">critical</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
939069106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">eliminate</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Stat5</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Raf/MEK</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">pathways</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">E2F</tok></cons> <tok cat="NN">regulation</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
939069107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">Protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PKB</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">IL-2</tok></cons></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="NN">PI3K</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
939069108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">active</tok> <cons cat="NP"><tok cat="NN">PKB</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">sufficient</tok></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">E2F</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
939069109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PI3K</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Rb</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cyclin</tok> <tok cat="NN">D3</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">degradation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p27kip1</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
939069110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">establish</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">crucial</tok> <tok cat="JJ">PI3K/PKB-mediated</tok> <tok cat="NN">link</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">teceptor</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok> <tok cat="NN">machinery</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
939483201@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Thiol</tok> <tok cat="NN">modulation</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">interleukin</tok> <tok cat="LRB">(</tok><tok cat="NN">IL</tok><tok cat="RRB">)</tok><tok cat="JJ">-1-mediated</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="JJ">receptor-associated</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@16-12-2010
939483202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-1</tok> <tok cat="NN">receptor</tok> <tok cat="NN">type</tok> <tok cat="CD">I</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-1RI</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">proteins</tok></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="VP"><tok cat="VBG">forming</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">complex</tok> <tok cat="NN">system</tok></cons></cons> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-1</tok></cons> <cons cat="VP"><tok cat="VBZ">exerts</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">various</tok> <tok cat="NNS">signals</tok></cons><cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@26@@oe@16-12-2010
939483203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="VBG">initiating</tok> <tok cat="NN">event</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">still</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">uncertain</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">recently</tok> <tok cat="VBN">described</tok></cons> <cons cat="NP"><tok cat="JJ">receptor-associated</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJS">earliest</tok> <tok cat="NNS">events</tok></cons> <cons cat="ADJP"><tok cat="JJ">detectable</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NNP">Martin</tok> <tok cat="FW">et</tok> <tok cat="FW">al.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Eur.J.Immunol.1994.24</tok></cons><tok cat="COLON">:</tok> <cons cat="NP"><tok cat="CD">1566</tok></cons></cons><tok cat="RRB">)</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
939483204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">IL-1</tok></cons> <tok cat="NN">signaling</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">commonly</tok></cons> <cons cat="VP"><tok cat="VBN">accompanied</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">oxidative</tok> <tok cat="NNS">processes</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">thought</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">subject</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">redox</tok> <tok cat="NN">regulation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
939483205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">IL-1RI-associated</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">target</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">redox</tok> <tok cat="NN">regulation</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">altered</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">kinase</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">influence</tok> <cons cat="NP"><tok cat="JJ">IL-1-mediated</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
939483206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">EL4</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IL-1</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><cons cat="NP"><tok cat="NN">pretreatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">compounds</tok></cons> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">influence</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">redox</tok> <tok cat="NN">status</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
939483207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Thiol</tok> <tok cat="NN">modifying</tok> <tok cat="NNS">agents</tok></cons> <cons cat="PP"><tok cat="IN">like</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">diamide</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">menadione</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">pyrrolidine</tok> <tok cat="NN">dithiocarbamate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PDTC</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">diethyl</tok> <tok cat="NN">dithiocarbamate</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">phenylarsine</tok> <tok cat="NN">oxide</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">IL-1-induced</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">IL-1RI-associated</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
939483208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">N-Acetylcysteine</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">alpha,alpha'-dipyridyl</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">aminotriazole</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">nitrofurantoin</tok></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">show</tok> <cons cat="NP"><tok cat="DT">any</tok> <tok cat="NN">effect</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
939483209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">PDTC</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">reversible</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">unless</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="NN">glutathione</tok> <tok cat="NN">synthesis</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">buthionine</tok> <tok cat="NN">sulfoximine</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
939483210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="VBN">described</tok> <tok cat="NNS">conditions</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="VP"><tok cat="VBD">prevented</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">IL-1RI-associated</tok> <tok cat="NN">kinase</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">similarly</tok></cons> <cons cat="VP"><tok cat="VBD">impaired</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">EL4</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
939483211@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">From</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">observations</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">free</tok> <tok cat="NNS">thiols</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-1RI</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">IL-1RI-associated</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">process</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">mandatory</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">IL-1</tok></cons> <tok cat="NN">signaling</tok></cons> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
939808701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Differanisole</tok> <tok cat="NN">A</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">novel</tok></cons> <cons cat="ADJP"><tok cat="JJ">antitumor</tok></cons></cons> <tok cat="NN">antibiotic</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">enhances</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">growth</tok> <tok cat="NN">inhibition</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">leukemia</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">9-cis</tok> <tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@16-12-2010
939808702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Differanisole</tok> <tok cat="NN">A</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">3,5-dichloro-2-hydroxy-4-methoxy-6-n-propylbenzoic</tok> <tok cat="NN">acid</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">growth</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">leukemia</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@16-12-2010
939808703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">compound</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">G1</tok> <tok cat="NN">arrest</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">granulocytic</tok> <tok cat="NN">differentiation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HL-60</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">differentiation-inducing</tok> <tok cat="NN">effect</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">modest</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
939808704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Differanisole</tok> <tok cat="DT">A</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">9-cis</tok> <tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">9cisRA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">synergistically</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">growth</tok></cons> <tok cat="CC">and</tok> <cons cat="NP" syn="COOD"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">functional</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">morphologic</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">differentiation</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HL-60</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NB4</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">combined</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">differanisole</tok> <tok cat="NN">A</tok></cons> <tok cat="CC">and</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">all-trans</tok> <tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">1alpha,25-dihydroxyvitamin</tok> <tok cat="NN">D3</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="RBR">less</tok> <tok cat="JJ">effective</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
939808705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="JJ">Similar</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">culture</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">leukemia</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">patient</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">promyelocytic</tok> <tok cat="NN">leukemia</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
939808706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">synergistic</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">growth</tok> <tok cat="NN">inhibition</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">required</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">simultaneous</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">differanisole</tok> <tok cat="NN">A</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">9cisRA</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
939808707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Differanisole</tok> <tok cat="NN">A</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">RXR-specific</tok> <tok cat="NN">ligand</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Ro47-5944</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">cooperatively</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">growth</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">combined</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">differanisole</tok> <tok cat="NN">A</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">RAR-specific</tok> <tok cat="NN">ligand</tok></cons> <cons cat="NP"><tok cat="NN">Am80</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">just</tok> <tok cat="JJ">additive</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
939808708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Differanisole</tok> <tok cat="NN">A</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">9cisRA</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><tok cat="NNS">implications</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">therapy</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">promyelocytic</tok> <tok cat="NN">leukemia</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
939816301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">ATF1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CREB</tok></cons></cons> <cons cat="VP"><tok cat="VBP">trans-activate</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">cycle</tok> <tok cat="JJ">regulated</tok> <tok cat="NN">histone</tok> <tok cat="NN">H4</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">distal</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">matrix</tok> <tok cat="JJ">associated</tok> <tok cat="NN">promoter</tok> <tok cat="NN">element</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@16-12-2010
939816302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Proteins</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ATF/CREB</tok> <tok cat="NN">class</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">stimulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">several</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="JJ">growth-related</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="JJ">A-related</tok> <tok cat="NN">cAMP</tok> <tok cat="NN">response</tok> <tok cat="NNS">elements</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@16-12-2010
939816303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">cycle</tok> <tok cat="JJ">regulated</tok> <tok cat="NN">histone</tok> <tok cat="NN">H4</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><cons cat="QP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok> <tok cat="CD">four</tok></cons> <cons cat="NP"><tok cat="JJ">principal</tok> <tok cat="VBG">cis-acting</tok> <tok cat="NNS">elements</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">mediate</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">G1/S</tok> <tok cat="NN">phase</tok> <tok cat="NN">control</tok></cons> <tok cat="CC">and/or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">enhancement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
939816304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><tok cat="JJ">protein-DNA</tok> <tok cat="NN">interaction</tok> <tok cat="NNS">assays</tok></cons></cons></cons> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">H4</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NN">ATF/CREB</tok> <tok cat="NN">recognition</tok> <tok cat="NNS">motifs</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">interact</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CREB</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">ATF1</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">ATF2</tok></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">ATF4/CREB2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
939816305@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="CD">One</tok> <cons cat="NP"><tok cat="NN">ATF/CRE</tok> <tok cat="NN">motif</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">distal</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="JJ">matrix-associated</tok> <tok cat="NN">Site</tok> <tok cat="CD">IV</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">second</tok> <tok cat="NN">motif</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">proximal</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="NN">Site</tok> <tok cat="CD">I</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
939816306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">Both</tok> <cons cat="NP"><tok cat="NN">ATF/CRE</tok> <tok cat="NNS">motifs</tok></cons></cons> <cons cat="VP"><tok cat="VBP">overlap</tok> <cons cat="NP"><tok cat="VBG">binding</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">multifunctional</tok> <tok cat="NN">YY1</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" id="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">nuclear</tok> <tok cat="NN">matrix</tok> <tok cat="JJ">associated</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
939816307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Subnuclear</tok> <tok cat="NN">fractionation</tok></cons> <cons cat="VP"><tok cat="VBZ">reveals</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="CD">two</tok> <cons cat="NP"><tok cat="NN">ATF1</tok> <tok cat="NNS">isoforms</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" id="i11" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">appear</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">differ</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">respect</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><tok cat="NN">DNA</tok> <cons cat="NP"><tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">partition</tok></cons></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">selectively</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">nuclear</tok> <cons cat="NP"><tok cat="NN">matrix</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">nonmatrix</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">compartments</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="JJ">consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">matrix</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
939816308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Site-directed</tok> <tok cat="JJ">mutational</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Site</tok> <tok cat="CD">I</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Site</tok> <tok cat="CD">IV</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">together</tok></cons> <cons cat="VP"><tok cat="VBP">support</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ATF1-</tok> <cons cat="ADJP" null="QSTN"></cons><cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">CREB-induced</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">trans-activation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">H4</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
939816309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">establish</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">ATF/CREB</tok> <tok cat="NNS">factors</tok></cons> <cons cat="ADVP"><tok cat="RB">functionally</tok></cons> <cons cat="VP"><tok cat="VBP">modulate</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">histone</tok> <tok cat="NN">H4</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">distal</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">proximal</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NN">promoter</tok> <tok cat="NNS">elements</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
939840401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-7</tok></cons></cons> <cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">heterodimerization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT5</tok> <tok cat="NNS">isoforms</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphoblasts</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
939840402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="PP"><tok cat="IN">Despite</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">differences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">responses</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="LRB">(</tok><tok cat="NN">IL</tok><tok cat="RRB">)</tok><tok cat="CD">-2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-7</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">both</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons> <cons cat="VP"><tok cat="VBP">modulate</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">functions</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NNS">transducers</tok> <tok cat="CC">and</tok> <tok cat="NNS">activators</tok> <tok cat="IN">of</tok> <tok cat="NN">transcription</tok> <tok cat="LRB">(</tok><tok cat="NN">STAT</tok><tok cat="RRB">)</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@36@@oe@16-12-2010
939840403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">contribution</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="NNS">isoforms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT5</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT5A</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT5B</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2-</tok> <cons cat="ADJP" null="QSTN"></cons><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">IL-7-induced</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">activation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphoblasts</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
939840404@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">Both</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">assembly</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT5A</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT5B</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">complexes</tok></cons> <cons cat="ADJP"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interferon-gamma</tok> <tok cat="NN">activation</tok> <tok cat="NN">sequence</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GAS</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok> <cons cat="PRN" ref="i30" null="ICH"></cons></cons><cons cat="VP"><tok cat="VBD">translocated</tok> <cons cat="PRN" id="i30"><tok cat="LRB">(</tok><cons cat="PP" adv="TMP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="CD">1</tok> <tok cat="NN">min</tok></cons></cons><tok cat="RRB">)</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2-</tok> <cons cat="ADJP" null="QSTN"></cons><cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">IL-7-treated</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
939840405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">kinetics</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">translocation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">delayed</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><cons cat="NP"><tok cat="JJ">IL-7-treated</tok></cons></cons> <cons cat="NP" null="QSTN"></cons></cons></cons></cons><cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">IL-2-treated</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
939840406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-7</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">equivalent</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT5A</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT5B</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">facilitate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NNS">STATs</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="VBN">immobilized</tok> <tok cat="NN">GAS</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
939840407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">Both</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-7</tok></cons></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">substantial</tok> <tok cat="NNS">amounts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT5A/STAT5B</tok> <tok cat="NN">heterodimerization</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
939840408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">constitutive</tok> <tok cat="NN">association</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT3</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">each</tok> <cons cat="NP"><tok cat="NN">STAT5</tok> <tok cat="NN">isomer</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
939840409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-7</tok></cons></cons> <cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">assembly</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NNS">heterodimers</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">similar</tok> <tok cat="NN">manner</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="JJ">subsequent</tok> <tok cat="JJ">cellular</tok> <tok cat="NNS">responses</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">driven</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">similar</tok> <tok cat="NNS">sets</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
939996101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">chronic</tok> <tok cat="JJ">lymphocytic</tok> <tok cat="NN">leukemia</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">contain</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">transducer</tok> <tok cat="CC">and</tok> <tok cat="NN">activator</tok> <tok cat="IN">of</tok> <tok cat="NN">transcription</tok> <tok cat="LRB">(</tok><tok cat="NN">STAT</tok><tok cat="RRB">)</tok> <tok cat="CD">1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT3</tok></cons></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <tok cat="VBN">phosphorylated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">serine</tok> <tok cat="NNS">residues</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@16-12-2010
939996102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">explore</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathogenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">chronic</tok> <tok cat="JJ">lymphocytic</tok> <tok cat="NN">leukemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CLL</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">one</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJR">more</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">signal</tok> <tok cat="NN">transducer</tok> <tok cat="CC">and</tok> <tok cat="NN">activator</tok> <tok cat="IN">of</tok> <tok cat="NN">transcription</tok> <tok cat="LRB">(</tok><tok cat="NN">STAT</tok><tok cat="RRB">)</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">abnormal</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">CLL</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@38@@oe@16-12-2010
939996103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">No</tok> <tok cat="JJ">constitutive</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">any</tok> <cons cat="NP"><tok cat="NN">STAT</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">CLL</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
939996104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">assess</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">serine</tok> <tok cat="NNS">residues</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT3</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">CLL</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">raised</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">antibodies</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i19"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i19" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="VP"><tok cat="VBP">recognize</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">STAT1</tok></cons> <cons cat="VP"><tok cat="VBN">phosphorylated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">ser-727</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">STAT3</tok></cons> <cons cat="VP"><tok cat="VBN">phosphorylated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">ser-727</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
939996105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">100</tok><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">CLL</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">n</tok></cons> <cons cat="ADJP"><tok cat="JJ">=</tok> <cons cat="NP"><tok cat="CD">32</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i10" role="SBJ"><cons cat="NP"><tok cat="NN">STAT1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT3</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <cons cat="VP"><tok cat="VBN">phosphorylated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">serine</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
939996106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="PP" role="PRD"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">contrast</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="JJ">normal</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">lymphocytes</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">CD5+)</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NNS">tonsils</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">absent</tok><cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
939996107@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="NN">Serine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT3</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">seen</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">occasionally</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">leukemias</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">universal</tok> <tok cat="NN">finding</tok></cons> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">CLL</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
939996108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">serine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NNS">STATs</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <tok cat="JJ">continuous</tok> <tok cat="NN">process</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">incubation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CLL</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">kinase</tok> <tok cat="NN">inhibitor</tok> <tok cat="NN">H7</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">led</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">dephosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NN">serine</tok> <tok cat="NNS">residues</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
939996109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">STAT</tok> <tok cat="NN">serine</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">CLL</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">has</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">identified</tok><cons cat="NP" ref="i14" null="NONE"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="S"><cons cat="NP" ref="i14" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" syn="COOD" role="PRD"><tok cat="DT">neither</tok> <cons cat="NP"><tok cat="JJ">mitogen-activated</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons> <tok cat="CC">nor</tok> <cons cat="NP"><tok cat="NN">pp70(s6k)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
939996110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">summary</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">constitutive</tok> <tok cat="NN">serine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT3</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="NN">CLL</tok> <tok cat="NNS">samples</tok></cons> <cons cat="VP"><tok cat="VBN">tested</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">date</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" id="i15" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">physiologic</tok> <tok cat="NN">significance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">modification</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">remains</tok> <cons cat="S"><cons cat="NP" ref="i15" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">determined</tok><cons cat="NP" ref="i15" null="NONE"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
940037201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Molecular</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">anoxia/reoxygenation-induced</tok> <tok cat="NN">neutrophil</tok> <tok cat="NN">adherence</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="VBN">cultured</tok> <tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@11@@oe@16-12-2010
940037202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">objectives</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="S" role="PRD"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">1</tok><tok cat="RRB">)</tok></cons> <tok cat="VB">determine</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">time</tok> <tok cat="NN">course</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">neutrophil</tok> <tok cat="NN">adhesion</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">monolayers</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">umbilical</tok> <tok cat="NN">vein</tok> <tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">HUVECs</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" id="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">exposed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">60</tok> <tok cat="NNS">minutes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">anoxia</tok></cons></cons> <cons cat="VP"><tok cat="VBN">followed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><cons cat="QP"><tok cat="CD">30</tok> <tok cat="TO">to</tok> <tok cat="CD">600</tok></cons> <tok cat="NNS">minutes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">reoxygenation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">2</tok><tok cat="RRB">)</tok></cons> <tok cat="VB">define</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="ADJP"><tok cat="JJ">responsible</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <tok cat="DT">the</tok> <cons cat="NP"><cons cat="ADJP"><cons cat="ADJP"><tok cat="JJ">early</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">minutes</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><cons cat="ADJP"><tok cat="JJ">late</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">hours</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><cons cat="NP"><tok cat="NN">hyperadhesivity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">postanoxic</tok> <tok cat="NNS">HUVECs</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">neutrophils</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@72@@oe@16-12-2010
940037203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="ADVP"><tok cat="RB">clearly</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">anoxia/reoxygenation</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">A/R</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">biphasic</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">neutrophil</tok> <tok cat="NN">adhesion</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NNS">HUVECs</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ"><tok cat="NN">peak</tok> <tok cat="NNS">responses</tok></cons> <cons cat="VP"><tok cat="VBG">occurring</tok> <cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="CD">30</tok> <tok cat="NNS">minutes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">phase</tok> <tok cat="CD">1</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">240</tok> <tok cat="NNS">minutes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">phase</tok> <tok cat="CD">2</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">reoxygenation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
940037204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Oxypurinol</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">catalase</tok></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="JJ">phase-1</tok> <tok cat="NN">adhesion</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">xanthine</tok> <tok cat="NN">oxidase</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">H2O2</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
940037205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">comparison</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">platelet</tok> <tok cat="NN">activating</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PAF</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">contributed</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="CC">both</tok> <tok cat="NNS">phases</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">neutrophil</tok> <tok cat="NN">adhesion</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
940037206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Anti-intercellular</tok> <tok cat="NN">adhesion</tok> <tok cat="NN">molecule-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ICAM-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">anti-P-selectin</tok> <tok cat="NNS">antibodies</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="JJ">monoclonal</tok> <tok cat="NNS">antibodies</tok></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP"><tok cat="NNS">mAbs</tok></cons><tok cat="RRB">]</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">attenuated</tok> <cons cat="NP"><tok cat="JJ">phase-1</tok> <tok cat="NN">neutrophil</tok> <tok cat="NN">adhesion</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="JJ">consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">roles</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">constitutively</tok> <tok cat="VBN">expressed</tok></cons> <cons cat="NP"><tok cat="NN">ICAM-1</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">enhanced</tok> <tok cat="NN">surface</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">preformed</tok> <cons cat="NP"><tok cat="NN">P-selectin</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
940037207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="NN">Phase-2</tok> <tok cat="NN">neutrophil</tok> <tok cat="NN">adhesion</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">attenuated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">anti-E-selectin</tok> <tok cat="NN">mAb</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">dominant</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">adhesion</tok> <tok cat="NN">molecule</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">late</tok> <tok cat="NN">phase</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
940037208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Pretreatment</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">actinomycin</tok> <tok cat="NN">D</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">cycloheximide</tok></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">competing</tok> <tok cat="NNS">ds-oligonucleotides</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappa</tok> <tok cat="NN">B</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activator</tok> <tok cat="NN">protein-1</tok></cons> <cons cat="NP"><tok cat="NN">cognate</tok> <tok cat="NN">DNA</tok> <tok cat="NNS">sequences</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP"><tok cat="VBD">attenuated</tok> <cons cat="NP"><tok cat="NN">phase-2</tok> <tok cat="NN">response</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="FW">de</tok> <tok cat="FW">novo</tok></cons> <tok cat="NN">macromolecule</tok> <tok cat="NN">synthesis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
940037209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Surface</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ICAM-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">P-selectin</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">E-selectin</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NNS">HUVECs</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">correlated</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">phase-1</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><tok cat="CD">-2</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NN">neutrophil</tok> <tok cat="NN">adhesion</tok> <tok cat="NNS">responses</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
940037210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Collectively</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">A/R</tok></cons> <cons cat="VP"><tok cat="VBZ">elicits</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">two-phase</tok> <tok cat="JJ">neutrophil-endothelial</tok> <tok cat="NN">cell</tok> <tok cat="NN">adhesion</tok> <tok cat="NN">response</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">transcription-independent</tok></cons> <cons cat="NP" ref="i24" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">transcription-dependent</tok></cons> <cons cat="NP" ref="i24" null="RNR"></cons></cons><cons cat="NP" id="i24"><cons cat="NP"><tok cat="NN">surface</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">different</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NN">cell</tok> <tok cat="NN">adhesion</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
940684801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">C/EBP</tok></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">corticotropin-releasing</tok> <tok cat="NN">hormone</tok> <tok cat="NN">gene</tok> <tok cat="NN">promoter</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@9@@oe@16-12-2010
940684802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">purpose</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">studies</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="S" role="PRD"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">identify</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">signalling</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">affected</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">corticotropin</tok> <tok cat="NN">releasing</tok> <tok cat="NN">hormone</tok> <tok cat="LRB">(</tok><tok cat="NN">CRH</tok><tok cat="RRB">)</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@30@@oe@16-12-2010
940684803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NNS">Fragments</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="CD">3.6</tok> <tok cat="NN">kb</tok></cons> <tok cat="NN">sequence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">CRH</tok> <tok cat="NN">gene</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">amplified</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">PCR</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">ligated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">upstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">CAT</tok> <tok cat="NN">reporter</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
940684804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">constructs</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">transfected</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">variety</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP" syn="COOD"><tok cat="CC">either</tok> <cons cat="ADVP"><tok cat="RB">alone</tok></cons> <tok cat="CC">or</tok> <cons cat="ADVP"><tok cat="RB">together</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <tok cat="NN">expression</tok> <tok cat="NNS">vectors</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
940684805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="JJ">Basal</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="CD">3070</tok> <tok cat="NN">bp</tok> <tok cat="NN">CRH</tok> <tok cat="NN">promoter</tok> <tok cat="NN">fragment</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">only</tok></cons> <cons cat="VP"><tok cat="VBN">seen</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">neuronal</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">lymphoblastoid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
940684806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="NN">Promoter</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">increased</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="NP"><tok cat="NN">C/EBPbeta</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-IL6</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RBR">more</tok> <tok cat="RB">strongly</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">C/EBPdelta</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-IL6beta</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
940684807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="VBN">Increased</tok> <cons cat="NP"><tok cat="NN">CRH</tok></cons> <tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="VBG">following</tok> <cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">ester</tok> <tok cat="NN">treatment</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">a</tok> <tok cat="JJ">dominant</tok> <tok cat="JJ">negative</tok> <cons cat="NP"><tok cat="NN">NF-IL6</tok> <tok cat="NN">mutant</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">showing</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">ester</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">principally</tok></cons> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">C/EBP</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
940684808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inverse</tok> <tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CRH</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">hypothalamus</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">spleens</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">arthritic</tok> <tok cat="NNS">rats</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">paralleled</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">similar</tok> <tok cat="NN">inverse</tok> <tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">NF-IL6beta</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">organs</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
940684809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">some</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok></cons> <tok cat="NN">signalling</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CRH</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
941399901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">novel</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">myeloid-specific</tok> <tok cat="NN">zinc</tok> <tok cat="NN">finger</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MZF-2</tok></cons><tok cat="RRB">)</tok></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
941399902@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">BACKGROUND</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="JJ">Myeloid</tok> <tok cat="NN">cell</tok> <tok cat="NN">development</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">controlled</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">tissue-specific</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@12@@oe@16-12-2010
941399903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Human</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">zinc</tok> <tok cat="NN">finger</tok> <tok cat="NN">protein</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MZF-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">putative</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="CD">13</tok> <tok cat="NN">zinc</tok> <tok cat="NNS">fingers</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">suggested</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">regulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">development</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">neutrophilic</tok> <tok cat="NNS">granulocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
941399904@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">RESULTS</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">murine</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">human</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">cDNAs</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">encode</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MZF</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MZF-2</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
941399905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">Murine</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">human</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">MZF-2</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">consisted</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">814</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">775</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NN">amino</tok> <tok cat="NNS">acids</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">identity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">75.3</tok><tok cat="NN">%</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="PRP">them</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
941399906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">C-terminal</tok> <tok cat="NN">half</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">MZF-2</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">carrying</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">zinc</tok> <tok cat="NN">finger</tok> <tok cat="NNS">domains</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">completely</tok> <tok cat="JJ">identical</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">MZF-1</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">N-terminal</tok> <tok cat="NN">half</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MZF-2</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">different</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">corresponding</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP"><tok cat="NN">MZF-1</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">coded</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">distinct</tok> <cons cat="NP"><tok cat="NNS">exons</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
941399907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="NN">MZF-2</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">particularly</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">committed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">neutrophilic</tok> <tok cat="NN">lineage</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">down-regulated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">G-CSF</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
941399908@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">CONCLUSIONS</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" syn="COOD" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">MZF-1</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">MZF-2</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NNS">mRNAs</tok></cons></cons> <cons cat="VP"><tok cat="VBP">seem</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">alternative</tok> <tok cat="NN">use</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">different</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">initiation</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
941399909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">distinct</tok> <cons cat="NP"><tok cat="JJ">N-terminal</tok> <tok cat="NN">half</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MZF-2</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">carries</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">characteristic</tok> <tok cat="NNS">domains</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">leucine-rich</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="VP"><tok cat="VBN">called</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NN">LeR</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">acidic</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">unique</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MZF-2</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">neutrophil</tok> <tok cat="NN">development</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
941412901@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">c-Rel</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p65</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">subunits</tok></cons></cons> <cons cat="VP"><tok cat="VBP">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">upstream</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">granulocyte</tok> <tok cat="NN">macrophage-colony</tok> <tok cat="NN">stimulating</tok> <tok cat="NN">factor</tok> <tok cat="NN">promoter</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">ester</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="CD">5637</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@26@@oe@16-12-2010
941412902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="ADVP"><tok cat="RB">further</tok></cons> <cons cat="VP"><tok cat="VB">clarify</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">complex</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">GM-CSF</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i24"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i24" id="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">extensively</tok></cons> <cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">studied</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">upstream</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="JJ">like</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="CD">5637</tok> <tok cat="JJ">non-lymphoid</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">derives</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">bladder</tok> <tok cat="NN">carcinoma</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <tok cat="VBZ">produces</tok> <cons cat="NP"><tok cat="NN">GM-CSF</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@51@@oe@16-12-2010
941412903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">This</tok> <tok cat="NN">sequence</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">named</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="DT">the</tok> <tok cat="NN">A</tok> <tok cat="NN">element</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">active</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GM-CSF</tok></cons> <tok cat="NN">transcription</tok></cons></cons> <cons cat="PP" ref="i30" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">responsive</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">promoter</tok> <tok cat="NN">PMA</tok></cons></cons></cons> <cons cat="PP" id="i30"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">transfection</tok> <tok cat="NNS">experiments</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
941412904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">describe</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">heterodimeric</tok> <tok cat="NN">binding</tok> <tok cat="NN">complex</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NNS">subunits</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Rel</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" syn="COOD" role="PRD"><cons cat="ADJP"><tok cat="JJ">identical</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">one</tok></cons> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" null="NONE"></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HIV-LTR-kappaB</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">recognition</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">different</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">one</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Rel</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p50</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">Mo</tok> <tok cat="NN">T-lymphoid</tok> <tok cat="NN">HTLV-II</tok> <tok cat="JJ">infected</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@50@@oe@16-12-2010
941563901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">reactivity</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">Sjogren</tok><tok cat="POS">'s</tok> <tok cat="NN">syndrome</tok> <tok cat="JJ">related</tok></cons> <tok cat="NN">antigen</tok></cons> <cons cat="NP"><tok cat="NN">La</tok><tok cat="LRB">(</tok><tok cat="NN">SSB</tok><tok cat="RRB">)</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@16-12-2010
941563902@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">OBJECTIVE</tok><tok cat="PERIOD">.</tok></cons></cons>@@@@1@2@@oe@16-12-2010
941563903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Many</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Sjogren</tok><tok cat="POS">'s</tok> <tok cat="NN">syndrome</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SS</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">make</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NN">titer</tok> <cons cat="NP"><tok cat="NN">IgG</tok> <tok cat="NNS">autoantibodies</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">La</tok><tok cat="LRB">(</tok><tok cat="NN">SSB</tok><tok cat="RRB">)</tok></cons> <tok cat="NN">antigen</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><tok cat="NN">antigen</tok> <tok cat="JJ">specific</tok> <tok cat="NN">T</tok> <tok cat="NN">cell-B</tok> <tok cat="NN">cell</tok> <tok cat="NNS">interactions</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
941563904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">responses</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">some</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">antigens</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">particularly</tok></cons> <cons cat="NP"><tok cat="NN">U1RNP</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">systemic</tok> <tok cat="NN">lupus</tok> <tok cat="NN">erythematosus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SLE</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">subjects</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
941563905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">reactivity</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">autoantigen</tok> <tok cat="NN">SSB</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">SS</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">controls</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
941563906@GENIA Treebank@formal@@1@S@<cons cat="NP"><tok cat="NNS">METHODS</tok><tok cat="PERIOD">.</tok></cons>@@@@1@2@@oe@16-12-2010
941563907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">[3H]thymidine</tok> <tok cat="NN">proliferation</tok> <tok cat="NN">assay</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">determined</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">reactivity</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">purified</tok> <tok cat="JJ">recombinant</tok> <tok cat="NN">SSB</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">rSSB</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="CD">20</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">SS</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">19</tok> <tok cat="NNS">controls</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
941563908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">Specificity</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">determined</tok> <cons cat="NP" ref="i6" null="NONE"></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">tetanus</tok> <tok cat="NN">toxoid</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">endotoxin</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">3</tok> <tok cat="JJ">other</tok> <cons cat="NP"><tok cat="NNS">autoantigens</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PBC.M2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Sc170</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">GAD</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
941563909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="NN">Precursor</tok> <tok cat="NN">frequency</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">calculated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">limiting</tok> <cons cat="NP"><tok cat="NN">dilution</tok> <tok cat="NN">analysis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
941563910@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="NN">HLA</tok> <tok cat="NN">Class</tok> <tok cat="CD">II</tok> <tok cat="NN">dependency</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="NP" ref="i9" null="NONE"></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="JJ">anti-Class</tok> <tok cat="CD">II</tok> <tok cat="JJ">monoclonal</tok> <tok cat="NNS">antibodies</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
941563911@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HLA-DR</tok> <tok cat="NN">typing</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="PP" role="PRD"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">polymerase</tok> <tok cat="NN">chain</tok> <tok cat="NN">reaction</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">sequence</tok> <tok cat="JJ">specific</tok> <tok cat="NN">oligonucleotide</tok> <tok cat="NN">typing</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
941563912@GENIA Treebank@formal@@1@S@<cons cat="NP"><tok cat="NNS">RESULTS</tok><tok cat="PERIOD">.</tok></cons>@@@@1@2@@oe@16-12-2010
941563913@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">Six</tok> <tok cat="IN">of</tok> <tok cat="CD">20</tok></cons> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">SS</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">10/19</tok> <tok cat="NNS">controls</tok></cons></cons> <cons cat="VP"><tok cat="VBD">proliferated</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">La</tok></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">rSSB</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
941563914@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Precursor</tok> <tok cat="NN">frequency</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">anti-SSB</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" syn="COOD" role="PRD"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">1:77,040</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">1:115,000</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="CD">2</tok> <tok cat="JJ">healthy</tok> <tok cat="NNS">subjects</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">1:230,250</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">1:103,034</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">SS</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
941563915@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Anti-HLA-DR</tok></cons> <cons cat="VP"><tok cat="VBD">abrogated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">proliferation</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">SSB</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">tetanus</tok> <tok cat="NN">toxoid</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
941563916@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">Thirteen</tok> <tok cat="IN">of</tok> <tok cat="CD">15</tok></cons> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">SS</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">4/17</tok> <tok cat="NNS">controls</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><cons cat="NP"><tok cat="NN">HLA-DR3</tok></cons> <tok cat="JJ">positive</tok><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="JJ">apparent</tok> <tok cat="NN">association</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HLA-DR3</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">SSB</tok></cons> <tok cat="NN">reactivity</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">controls</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
941563917@GENIA Treebank@formal@@1@S@<cons cat="NP"><tok cat="NN">CONCLUSION</tok><tok cat="PERIOD">.</tok></cons>@@@@1@2@@oe@16-12-2010
941563918@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Anti-La(SSB)</tok> <tok cat="JJ">specific</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">occur</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">significant</tok> <tok cat="NN">proportion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">controls</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">some</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">SS</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
941563919@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i15" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">SSB</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">controls</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">remains</tok> <cons cat="S"><cons cat="NP" ref="i15" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">defined</tok><cons cat="NP" ref="i15" null="NONE"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
941563920@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">They</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">represent</tok> <cons cat="NP"><tok cat="JJ">immunoregulatory</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">further</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">comparison</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">SS</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">elucidate</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">immunoregulation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">derangements</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">lead</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">Sjogren</tok><tok cat="POS">'s</tok> <tok cat="NN">syndrome</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
941688701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Cyclosporin</tok> <tok cat="NN">A</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">monocyte</tok> <cons cat="NP"><tok cat="NN">tissue</tok> <tok cat="NN">factor</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">cardiac</tok> <tok cat="NN">transplant</tok> <tok cat="NNS">recipients</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@12@@oe@16-12-2010
941688702@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">BACKGROUND</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" syn="COOD" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">Fibrin</tok> <tok cat="NN">deposition</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">thrombosis</tok></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">allograft</tok> <tok cat="NN">rejection</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">vasculopathy</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="JJ">cardiac</tok> <tok cat="NN">transplantation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
941688703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Because</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">monocytes</tok></cons> <cons cat="VP"><tok cat="VBP">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">pivotal</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathophysiology</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">intravascular</tok> <tok cat="NN">coagulation</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">synthesize</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tissue</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TF</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">asked</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">1</tok><tok cat="RRB">)</tok></cons> <tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">monocyte</tok> <cons cat="NP"><tok cat="NN">TF</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">cardiac</tok> <tok cat="NN">transplant</tok> <tok cat="NNS">recipients</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">2</tok><tok cat="RRB">)</tok></cons> <tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">monocyte</tok> <cons cat="NP"><tok cat="NN">TF</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">affected</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cyclosporin</tok> <tok cat="NN">A</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CsA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@57@@oe@16-12-2010
941688704@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">METHODS</tok></cons> <tok cat="CC">AND</tok> <cons cat="NP"><tok cat="NNS">RESULTS</tok></cons><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">measured</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">TF</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="RB">highly</tok> <tok cat="VBN">purified</tok> <tok cat="NNS">monocytes/macrophages</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">10</tok> <tok cat="JJ">consecutive</tok> <tok cat="JJ">cardiac</tok> <tok cat="NN">transplant</tok> <tok cat="NNS">recipients</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">10</tok> <tok cat="JJ">healthy</tok> <tok cat="JJ">control</tok> <tok cat="NNS">subjects</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
941688705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">TF</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBN">generated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="ADJP"><cons cat="NP"><tok cat="JJ">unstimulated</tok></cons></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">endotoxin-stimulated</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="RB">significantly</tok> <tok cat="JJR">higher</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">transplant</tok> <tok cat="NNS">recipients</tok></cons></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">control</tok> <tok cat="NNS">subjects</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">P</tok></cons><cons cat="ADJP"><tok cat="JJR">&lt;</tok><cons cat="NP"><tok cat="CD">.05</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
941688706@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="VBN">Increased</tok> <tok cat="NN">monocyte</tok> <cons cat="NP"><tok cat="NN">TF</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">transplant</tok> <tok cat="NNS">recipients</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADVP"><tok cat="RB">adversely</tok></cons> <cons cat="VP"><tok cat="VBN">affected</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">CsA</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">TF</tok></cons> <tok cat="NN">induction</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">markedly</tok></cons> <cons cat="VP"><tok cat="VBN">reduced</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">CsA</tok> <tok cat="NN">serum</tok> <tok cat="NNS">concentrations</tok></cons> <cons cat="VP"><tok cat="VBG">reaching</tok> <cons cat="NP"><tok cat="JJ">peak</tok> <tok cat="NN">CsA</tok> <tok cat="NN">drug</tok> <tok cat="NNS">levels</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
941688707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">TF</tok></cons> <tok cat="NN">induction</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NN">CsA</tok> <tok cat="NN">blood</tok> <tok cat="NNS">concentrations</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i10" null="NONE"></cons></cons><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i11"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">performed</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">interferon-gamma</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">interleukin-1beta</tok></cons></cons></cons><cons cat="ADVP" ref="i11" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
941688708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">As</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="JJ">reverse</tok> <tok cat="NN">transcription-polymerase</tok> <tok cat="NN">chain</tok> <tok cat="NN">reaction</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">CsA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">decreased</tok> <cons cat="NP"><tok cat="NN">TF</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="NN">expression</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">reduced</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i14"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i14" id="i15" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" ref="i15" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TF</tok> <tok cat="NN">promotor</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@49@@oe@16-12-2010
941688709@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">CONCLUSIONS</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBZ">demonstrates</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i16" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">TF</tok></cons> <tok cat="NN">activation</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">occurring</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">cardiac</tok> <tok cat="NN">transplant</tok> <tok cat="NNS">recipients</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i16" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">CsA</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
941688710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">monocyte</tok> <cons cat="NP"><tok cat="NN">TF</tok></cons> <tok cat="NN">induction</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">CsA</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">successful</tok> <tok cat="NN">use</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">cardiac</tok> <tok cat="NN">transplant</tok> <tok cat="NN">medicine</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">useful</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">managing</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">further</tok> <tok cat="NNS">settings</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">vascular</tok> <tok cat="NN">pathology</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">involve</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">TF</tok></cons> <tok cat="NN">expression</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
941813501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Selection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">diverse</tok> <cons cat="NP"><tok cat="NN">TCR</tok> <tok cat="NN">repertoire</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="JJ">virus-encoded</tok> <tok cat="NN">transactivator</tok> <tok cat="NN">protein</tok></cons> <cons cat="NP"><tok cat="NN">BZLF1</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">CD8+</tok> <tok cat="JJ">cytotoxic</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">primary</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">persistent</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">infection</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@26@@oe@16-12-2010
941813502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">CD8+</tok> <tok cat="JJ">cytotoxic</tok> <tok cat="NN">T</tok> <tok cat="NN">lymphocyte</tok> <tok cat="LRB">(</tok><tok cat="NN">CTL</tok><tok cat="RRB">)</tok> <tok cat="NN">repertoire</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">HLA</tok> <tok cat="JJ">B8-restricted</tok> <tok cat="NN">peptide</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">RAKFKQLLQ</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok> <tok cat="LRB">(</tok><tok cat="NN">EBV</tok><tok cat="RRB">)</tok> <tok cat="JJ">immediate-early</tok> <tok cat="NN">protein</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">BZLF1</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@32@@oe@16-12-2010
941813503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="JJ">Repertoire</tok> <tok cat="NN">selection</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">monitored</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">determining</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TCR</tok> <tok cat="NN">beta</tok> <tok cat="NN">chain</tok> <tok cat="NNS">sequences</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">RAKFKQLLQ-specific</tok> <tok cat="NN">CTL</tok></cons></cons> <cons cat="VP"><tok cat="VBN">established</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">primary</tok> <tok cat="JJ">infected</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NN">virus</tok> <tok cat="NNS">carriers</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
941813504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">PCR</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">spontaneous</tok> <tok cat="JJ">EBV-transformed</tok> <tok cat="JJ">lymphoblastoid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">LCL</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok> <tok cat="NNS">individuals</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">infection</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="CD">two</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">infected</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP" id="i31"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">type</tok> <tok cat="NN">A</tok> <cons cat="NP" null="QSTN"></cons></cons></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="S"><cons map="i6" cat="NP" role="SBJ"><tok cat="CD">one</tok></cons> <cons map="i31" cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">type</tok> <tok cat="NN">B</tok> <tok cat="NN">EBV</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
941813505@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">Polyclonal</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">clonal</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">CTL</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" id="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">generated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">stimulating</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">HLA</tok> <tok cat="JJ">B8+</tok> <tok cat="JJ">homozygous</tok> <tok cat="NNS">LCL</tok> <tok cat="JJ">lysed</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">blasts</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="JJ">pulsed</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">peptide</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">RAKFKQLLQ</tok></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="NN">lysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">certain</tok> <tok cat="NN">HLA</tok> <tok cat="JJ">B8+</tok> <tok cat="NNS">LCL</tok> <tok cat="NNS">targets</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">abundance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">BZLF1</tok> <tok cat="NNS">transcripts</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@16-12-2010
941813506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">TCR</tok> <tok cat="NN">beta</tok></cons> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><tok cat="NN">loop</tok> <tok cat="NN">length</tok> <tok cat="NN">restriction</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">putative</tok> <tok cat="NN">peptide</tok> <tok cat="NN">contact</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">all</tok> <tok cat="VBG">responding</tok> <cons cat="NP"><tok cat="NN">beta</tok> <tok cat="NNS">chains</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i11" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">diverse</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">unique</tok> <tok cat="LRB">(</tok><tok cat="JJ">non-recurrent</tok><tok cat="RRB">)</tok> <tok cat="NN">TCR</tok> <tok cat="NN">beta</tok> <tok cat="NNS">clonotypes</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">selected</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">individuals</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">infection</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">continued</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">emerge</tok> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="JJ">long-term</tok> <tok cat="NN">virus</tok> <tok cat="NN">exposure</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@48@@oe@16-12-2010
941813507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="JJ">TCR-contact</tok> <tok cat="NN">site</tok> <tok cat="NN">heterogeneity</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">excluded</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">selective</tok> <tok cat="NN">force</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">diversity</tok> <tok cat="NN">generation</tok></cons></cons></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">since</tok> <cons cat="S"><cons cat="NP" id="i14" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">epitope-encoded</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i14" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">identical</tok> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="JJ">endogenous</tok> <tok cat="NN">virus</tok> <tok cat="NNS">isolates</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
941813508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">first</tok> <tok cat="NN">study</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">TCR</tok> <tok cat="NN">repertoire</tok></cons> <tok cat="NN">selection</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">EBV</tok> <tok cat="JJ">lytic</tok> <tok cat="NN">antigen</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i16" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">BZLF1-reactive</tok> <tok cat="NN">component</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">diverse</tok> <tok cat="NNS">clonotypes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i16" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="JJ">primary</tok> <cons cat="NP"><tok cat="NN">type</tok> <tok cat="NN">A</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">type</tok> <tok cat="NN">B</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NN">EBV</tok> <tok cat="NN">infection</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i17"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i17" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">sustained</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">EBV-specific</tok> <tok cat="NN">memory</tok> <tok cat="NN">response</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">throughout</tok> <cons cat="NP"><tok cat="JJ">life-long</tok> <tok cat="NN">infection</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
941813509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i19" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">diversity</tok> <tok cat="NN">selection</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">likely</tok> <cons cat="S"><cons cat="NP" ref="i19" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">critical</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">maintaining</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">balanced</tok></cons> <cons cat="ADJP"><tok cat="JJ">viral</tok></cons></cons> <tok cat="NN">load</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">throughout</tok> <cons cat="NP"><tok cat="NN">EBV</tok> <tok cat="NN">persistence</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
941943001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Agonistic</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">CD40-specific</tok> <tok cat="JJ">single-chain</tok> <tok cat="NN">Fv</tok></cons></cons> <cons cat="VP"><tok cat="VBN">constructed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">variable</tok> <tok cat="NNS">regions</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">mAb</tok> <tok cat="NN">G28-5</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@16-12-2010
941943002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i15" role="SBJ"><tok cat="DT">A</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">single-chain</tok> <tok cat="NN">Fv</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">sFv</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">variable</tok> <tok cat="NNS">regions</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">CD40-specific</tok> <cons cat="NP"><tok cat="NN">mAb</tok> <tok cat="NN">G28-5</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@16-12-2010
941943003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">molecule</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">bound</tok> <cons cat="NP"><tok cat="NN">CD40</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">high</tok> <tok cat="NN">affinity</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">2.2</tok> <tok cat="NN">nM</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <tok cat="NN">monomer</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">solution</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
941943004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Surprisingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">G28-5</tok> <tok cat="NN">sFv</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potent</tok> <cons cat="NP"><tok cat="NN">CD40</tok> <tok cat="NN">agonist</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok></cons> <tok cat="VBD">crosslinked</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD40</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">surface</tok></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">crosslink</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD40-Ig</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">solution</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
941943005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">G28-5</tok> <tok cat="NN">sFv</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RBR">more</tok> <tok cat="JJ">potent</tok></cons> <tok cat="NN">agonist</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><tok cat="NN">G28-5</tok> <tok cat="NN">IgG</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">stimulate</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">CD40</tok></cons> <tok cat="NNS">responses</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
941943006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">G28-5</tok> <tok cat="NN">IgG</tok></cons> <cons cat="ADVP"><tok cat="RB">partially</tok></cons> <cons cat="VP"><tok cat="VBD">blocked</tok><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">G28-5</tok> <tok cat="NN">sFv</tok></cons> <cons cat="VP"><tok cat="VBD">augmented</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD40</tok></cons> <tok cat="NNS">responses</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">natural</tok> <tok cat="NN">ligand</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">gp39-CD8</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
941943007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">functional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">ligands</tok></cons> <cons cat="VP"><tok cat="VBN">built</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="VBG">binding</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">G28-5</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">highly</tok> <tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">size</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">physical</tok> <tok cat="NNS">properties</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">molecule</tok></cons></cons></cons></cons> <cons cat="PP" syn="COOD"><tok cat="CC">both</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">solution</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NNS">surfaces</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
942753301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Competent</tok> <tok cat="NN">transcription</tok> <tok cat="NN">initiation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">RNA</tok> <tok cat="NN">polymerase</tok> <tok cat="CD">II</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cell-free</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><tok cat="NN">xeroderma</tok> <tok cat="NN">pigmentosum</tok> <tok cat="NNS">groups</tok> <cons cat="NP"><tok cat="NN">B</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">D</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="VBN">optimized</tok> <tok cat="NN">RNA</tok> <tok cat="NN">transcription</tok> <tok cat="NN">assay</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@16-12-2010
942753302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">human</tok> <tok cat="JJ">autosomal</tok> <tok cat="JJ">recessive</tok> <tok cat="NN">disease</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">xeroderma</tok> <tok cat="NN">pigmentosum</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">XP</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">result</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">mutations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">any</tok> <tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">seven</tok> <tok cat="NNS">genes</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">designated</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><cons cat="NP"><tok cat="NN">XPA</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="NN">XPG</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@28@@oe@16-12-2010
942753303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Of</tok> <cons cat="NP"><tok cat="DT">these</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">XPB</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">XPD</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">genes</tok></cons></cons> <cons cat="VP"><tok cat="VBP">encode</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">proteins</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><cons cat="NP"><tok cat="NNS">subunits</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">general</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">TFIIH</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">nucleotide</tok> <tok cat="NN">excision</tok> <tok cat="NN">repair</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NER</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">initiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">mRNA</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">RNA</tok> <tok cat="NN">polymerase</tok> <tok cat="CD">II</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
942753304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NNS">humans</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">mutation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">XPB</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">XPD</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">gene</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">impairs</tok> <cons cat="NP"><tok cat="NN">NER</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">hyper-sensitivity</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">sunlight</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">greatly</tok> <tok cat="VBN">increased</tok></cons> <tok cat="NN">skin</tok> <tok cat="NN">tumor</tok> <tok cat="NN">formation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
942753305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><tok cat="DT">no</tok> <tok cat="NN">transcription</tok> <tok cat="NN">deficiency</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="NN">XP-B</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">XP-D</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
942753306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">employed</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="VBN">optimized</tok> <tok cat="JJ">cell-free</tok> <tok cat="NN">RNA</tok> <tok cat="NN">transcription</tok> <tok cat="NN">assay</tok></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">analyze</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">XP-B</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">XP-D</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
942753307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">growth</tok> <tok cat="NN">rate</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">normal</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">XP-B</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">XP-D</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">reduced</tok> <tok cat="NNS">amounts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TFIIH</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
942753308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NNS">Extracts</tok></cons> <cons cat="VP"><tok cat="VBN">prepared</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">XP-B</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">XP-D</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="JJ">lymphoblastoid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">exhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">similar</tok> <tok cat="NN">transcription</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">adenovirus</tok> <tok cat="JJ">major</tok> <tok cat="JJ">late</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i8"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i8" null="T"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
942753309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">XP-B</tok> <cons cat="NP" ref="i24" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">XP-D</tok> <cons cat="NP" ref="i24" null="RNR"></cons></cons><cons cat="NP" id="i24"><tok cat="JJ">lymphoblastoid</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBP">do</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><tok cat="JJ">impaired</tok> <tok cat="NN">RNA</tok> <tok cat="NN">transcription</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
942753310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">consider</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">possible</tok> <tok cat="NNS">consequences</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">reduced</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">content</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TFIIH</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">clinical</tok> <tok cat="NNS">symptoms</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">XP-B</tok> <cons cat="NP" ref="i25" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">XP-D</tok> <cons cat="NP" ref="i25" null="RNR"></cons></cons><cons cat="NP" id="i25"><tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">discuss</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="LQT">'</tok><tok cat="JJ">conditional</tok> <tok cat="NN">phenotype</tok><tok cat="RQT">'</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i11"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">involve</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">impairment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NN">function</tok></cons></cons></cons> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <tok cat="IN">under</tok> <cons cat="NP"><tok cat="JJ">certain</tok> <tok cat="NN">growth</tok> <tok cat="NNS">conditions</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
942879301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Cyclosporin</tok> <tok cat="NN">A</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">G0/G1</tok> <tok cat="NN">switch</tok> <tok cat="NN">gene</tok> <tok cat="CD">2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">G0S2</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">cultured</tok> <tok cat="JJ">human</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@16-12-2010
942879302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="NN">Cyclosporin</tok> <tok cat="NN">A</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CsA</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">achieve</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">immunosuppressive</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inhibiting</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">calcium-</tok> <tok cat="CC">and</tok> <tok cat="JJ">calmodulin-dependent</tok> <tok cat="NN">phosphatase</tok></cons></cons> <cons cat="NP"><tok cat="NN">calcineurin</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" id="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">target</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NFAT</tok> <tok cat="LRB">(</tok><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="IN">of</tok> <tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok><tok cat="RRB">)</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">family</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@43@@oe@16-12-2010
942879303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" tpc="TPC" id="i7" role="PRD"><tok cat="IN">Among</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NN">target</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="PP" ref="i7" role="PRD" null="T"></cons></cons><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">gene</tok></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL2</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">cytokine</tok></cons></cons> <cons cat="VP"><tok cat="VBG">facilitating</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">progression</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">G1</tok> <tok cat="NN">phase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
942879304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">IL2</tok></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">reverse</tok> <cons cat="NP"><tok cat="NN">CsA</tok> <tok cat="NN">inhibition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="QP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok> <tok cat="CD">one</tok></cons> <tok cat="JJ">other</tok> <cons cat="NP"><tok cat="JJ">NFAT-sensitive</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">involved</tok><cons cat="NP" ref="i8" null="NONE"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
942879305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">G0/G1</tok> <tok cat="NN">switch</tok> <tok cat="NN">gene</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">G0S2</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><tok cat="JJ">potential</tok> <cons cat="NP"><tok cat="JJ">NFAT-binding</tok> <tok cat="NNS">sites</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">5'</tok> <tok cat="NN">flank</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">encodes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">small</tok> <tok cat="JJ">basic</tok> <tok cat="JJ">potential</tok> <tok cat="NN">phosphoprotein</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">unknown</tok> <tok cat="NN">function</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
942879306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">sensitive</tok><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">reverse</tok> <tok cat="NN">transcription-polymerase</tok> <tok cat="NN">chain</tok> <tok cat="NN">reaction</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RT-PCR</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">assay</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="NN">G0S2</tok></cons> <tok cat="NN">mRNA</tok> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">assayed</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">cultured</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
942879307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP"><tok cat="RB">Freshly</tok> <tok cat="VBN">isolated</tok></cons> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">contain</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">G0S2</tok> <tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i11"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="T"></cons><cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok></cons> <cons cat="VP"><tok cat="VBP">decline</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
942879308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="DT">This</tok> <tok cat="LQT">"</tok><tok cat="JJ">spontaneous</tok> <tok cat="NN">stimulation</tok><tok cat="RQT">"</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">noted</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">some</tok> <tok cat="JJ">other</tok> <cons cat="NP"><tok cat="NN">G0S</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">attributed</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">some</tok> <tok cat="NN">aspect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">isolation</tok> <tok cat="NN">procedure</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
942879309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i13"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i13" id="i14" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">preincubated</tok> <cons cat="NP" ref="i14" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">lower</tok> <cons cat="NP"><tok cat="NN">mRNA</tok> <tok cat="NNS">levels</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">transient</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">G0S2</tok> <tok cat="NN">mRNA</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">peaking</tok> <cons cat="PP" adv="TMP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="CD">1-2</tok> <tok cat="NN">h</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Concanavalin-A</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ConA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">ester</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TPA</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">calcium</tok> <tok cat="NN">ionophore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">ionomycin</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@16-12-2010
942879310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i15" role="SBJ"><tok cat="PDT">Both</tok> <tok cat="DT">these</tok> <tok cat="NNS">responses</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">CsA</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@16-12-2010
942879311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i16" role="SBJ"><cons cat="NP"><tok cat="NN">G0S2</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i16" null="NONE"></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">commit</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">enter</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">G1</tok> <tok cat="NN">phase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok><cons cat="S"><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" ref="i17" role="SBJ" null="NONE"></cons><tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBG">excluding</tok> <cons cat="NP"><tok cat="DT">other</tok> <tok cat="JJ">possible</tok> <tok cat="NNS">targets</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i17" role="SBJ"><cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">G0S2</tok></cons> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">CsA</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">achieving</tok> <cons cat="NP"><tok cat="NN">immunosuppression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
942879312@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">G0S2</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="PP" role="PRD"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">value</tok></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">analyzing</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">action</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CsA</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">influence</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">positive</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">negative</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">selection</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">self</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">not-self</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">antigens</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
942879601@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Estrogen</tok> <tok cat="NN">receptor</tok></cons> <cons cat="VP"><tok cat="VBZ">diminishes</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NNS">activities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">chicken</tok> <tok cat="NN">GATA-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><cons cat="NP"><tok cat="JJ">CACCC-binding</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@12@@oe@16-12-2010
942879602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">estrogen</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ER</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">repressed</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NN">differentiation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">erythroid-specific</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@16-12-2010
942879603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">ER</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="VP"><tok cat="VBN">referred</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" role="SBJ" null="NONE"></cons></cons><cons cat="ADVP"><tok cat="RB">throughout</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">ER</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NNS">activities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">erythroid-specific</tok> <tok cat="NNS">genes</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">particular</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">histone</tok> <tok cat="NN">H5</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
942879604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">rabbit</tok> <tok cat="NN">reticulocyte</tok> <tok cat="NN">lysate</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="JJ">human</tok> <tok cat="NN">ER</tok></cons> <cons cat="VP"><tok cat="VBD">reduced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok> <tok cat="NNS">activities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">chicken</tok> <tok cat="JJ">immature</tok> <tok cat="NN">erythrocyte</tok> <tok cat="JJ">nuclear</tok> <tok cat="JJ">extracted</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">GATA</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CACCC</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">sites</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <tok cat="NN">H5</tok> <cons cat="NP"><tok cat="NN">promoter</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">enhancer</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
942879605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok> <tok cat="NNS">activities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Sp1</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">affected</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">ER</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
942879606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">Binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ER</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">estrogen</tok> <tok cat="NN">response</tok> <tok cat="NN">element</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">enhanced</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">rabbit</tok> <tok cat="NN">reticulocyte</tok> <tok cat="NN">lysate</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
942879607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">lysate</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">necessary</tok> <cons cat="SBAR"><tok cat="IN">for</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">ER</tok></cons> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">diminish</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
942879608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">additional</tok> <tok cat="NN">factor</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">necessary</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">full</tok> <cons cat="NP"><tok cat="NN">ER</tok></cons> <tok cat="NN">function</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
942879609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">Both</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GATA-1</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">CACCC-binding</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">critical</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">developmentally</tok> <tok cat="VBN">regulated</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">erythroid-specific</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
942879610@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">hypothesize</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">interference</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NNS">activities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">GATA-1</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">CACCC-binding</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">ER</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">genes</tok></cons></cons></cons><cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
942892101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HLA</tok> <tok cat="NN">binding</tok> <tok cat="NNS">characteristics</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">generation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cytotoxic</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">peptides</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">oncogenic</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
942892102@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="S"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">AIMS</tok></cons> <tok cat="CC">AND</tok> <cons cat="NP"><tok cat="NN">BACKGROUND</tok></cons><tok cat="COLON">:</tok></cons> <cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">Structurally</tok> <tok cat="VBN">altered</tok></cons> <tok cat="NNS">proteins</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">chromosomal</tok> <tok cat="NNS">translocations</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NNS">mutations</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">considered</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NN">tumor</tok> <tok cat="JJ">specific</tok> <tok cat="NNS">antigens</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">represent</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">attractive</tok> <tok cat="NN">target</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">T-cell</tok> <tok cat="JJ">mediated</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@33@@oe@16-12-2010
942892103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBP">recognize</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">antigens</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">peptides</tok></cons> <cons cat="VP"><tok cat="VBN">bound</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NNS">HLA-molecules</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
942892104@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">MATERIALS</tok></cons> <tok cat="CC">AND</tok> <cons cat="NP"><tok cat="NNS">METHODS</tok></cons><tok cat="COLON">:</tok></cons> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NNS">Peptides</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">oncogenic</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">screened</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HLA</tok> <tok cat="NN">binding</tok> <tok cat="NNS">motifs</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="JJ">actual</tok> <tok cat="NN">binding</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">evaluated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">HLA</tok> <tok cat="NN">stabilization</tok> <tok cat="NNS">experiments</tok></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">transfectants</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <cons cat="NP"><tok cat="JJ">anti-HLA</tok> <tok cat="NNS">antibodies</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
942892105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="JJ">Specific</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">peptide</tok> <tok cat="NN">sensitization</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">screened</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">thymidine</tok> <tok cat="NN">uptake</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="JJ">cytotoxic</tok> <tok cat="NNS">assays</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
942892106@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">RESULTS</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">peptides</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">EWS/FLI-1</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="VBN">mutated</tok> <tok cat="NN">K-RAS</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="VP"><tok cat="VBG">encompassing</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">fusion</tok> <tok cat="NN">point</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mutation</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="NN">position</tok> <tok cat="CD">12</tok></cons></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">binding</tok> <tok cat="NN">motif</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HLA-Cw*0702</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HLA-A3</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
942892107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">actual</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">peptides</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">analysed</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">stabilization</tok> <tok cat="NN">assay</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
942892108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">detected</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">EWS/FLI-1</tok> <tok cat="NN">peptide</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">5</tok> <tok cat="NN">RAS</tok> <tok cat="NNS">peptides</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">1</tok> <tok cat="JJ">wild</tok> <tok cat="NN">type</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">4</tok> <tok cat="VBN">mutated</tok><cons cat="NP" null="QSTN"></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
942892109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">temperature</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">beta</tok> <tok cat="NN">2-microglobulin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">beta</tok> <tok cat="NN">2-m</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">fetal</tok> <tok cat="NN">calf</tok> <tok cat="NN">serum</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">FCS</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">stability</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HLA/peptide</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">studied</tok><cons cat="NP" ref="i12" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
942892110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">low</tok> <tok cat="NN">temperature</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">26</tok> <tok cat="NNS">degrees</tok> <tok cat="NN">C</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="CC">both</tok> <tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HLA-A3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HLA-Cw*0702</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">FCS</tok></cons> <cons cat="VP"><tok cat="VBD">reduced</tok> <cons cat="NP"><tok cat="PRP">it</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
942892111@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">beta</tok> <tok cat="NN">2-m</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HLA-A3</tok> <tok cat="NN">molecule</tok></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">influence</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HLA-Cw*0702</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
942892112@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">stability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="RB">already</tok> <tok cat="VBN">formed</tok> <tok cat="VBN">complexed</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">somewhat</tok> <tok cat="JJ">different</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HLA-A3</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HLA-Cw*0702</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NN">system</tok></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">both</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="RBR">more</tok> <tok cat="JJ">stable</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="CD">26</tok> <tok cat="NNS">degrees</tok> <tok cat="NN">C</tok></cons></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="CD">37</tok> <tok cat="NNS">degrees</tok> <tok cat="NN">C</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">beta</tok> <tok cat="NN">2-m</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">FCS</tok></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">influence</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">stability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HLA-A3/peptide</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="S"><cons cat="NP" id="i15" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBD">seemed</tok> <cons cat="S"><cons cat="NP" ref="i15" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">cause</tok> <cons cat="NP"><tok cat="JJ">opposite</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HLA-Cw*0702</tok></cons> <tok cat="NN">system</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">beta</tok> <tok cat="NN">2-m</tok></cons> <cons cat="VP"><tok cat="VBD">stabilized</tok> <cons cat="NP" ref="i30" null="RNR"></cons></cons></cons><tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">FCS</tok></cons> <cons cat="VP"><tok cat="VBD">destabilized</tok> <cons cat="NP" id="i30"><tok cat="DT">the</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@67@@oe@16-12-2010
942892113@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i18" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i18" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">generate</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">specific</tok> <cons cat="NP"><tok cat="JJ">CD8+</tok> <tok cat="NN">CTL</tok> <tok cat="NN">line</tok></cons></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">K-RAS</tok> <tok cat="JJ">mutated</tok> <tok cat="NN">peptide</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
942892114@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">CONCLUSIONS</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i19" role="SBJ"><cons cat="NP"><tok cat="VBG">binding</tok> <tok cat="NNS">motifs</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">actual</tok> <tok cat="NN">HLA</tok> <tok cat="NN">binding</tok></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i19" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NNS">cases</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">generation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">response</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">infrequent</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">confirming</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HLA</tok> <tok cat="NN">binding</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" syn="COOD" role="PRD"><cons cat="ADJP"><tok cat="JJ">necessary</tok></cons> <tok cat="CC">but</tok> <cons cat="ADJP"><tok cat="RB">not</tok> <tok cat="JJ">sufficient</tok></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">obtain</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">response</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
942892115@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i25" role="SBJ"><cons cat="NP"><tok cat="RB">Further</tok> <tok cat="NN">optimization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">culture</tok> <tok cat="NNS">conditions</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">type</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">Antigen</tok> <tok cat="NN">Presenting</tok> <tok cat="NNS">Cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">APC</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNS">peptides</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">use</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">stabilizers</tok></cons> <cons cat="PP"><tok cat="IN">like</tok> <cons cat="NP"><tok cat="NN">beta</tok> <tok cat="NN">2-m</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">still</tok></cons> <cons cat="VP"><tok cat="VBN">needed</tok><cons cat="NP" ref="i25" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
942899201@GENIA Treebank@formal@@1@S@<cons cat="UCP"><cons cat="UCP" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Constitutive</tok> <tok cat="NN">expression</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-fos</tok><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">c-jun</tok><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="PP" role="PRD"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">nucleated</tok> <tok cat="JJ">fetal</tok> <tok cat="NN">blood</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">up-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-jun</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">anti-CD3</tok></cons> <tok cat="NN">stimulation</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@16-12-2010
942899202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" id="i4" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">Fetal</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">neonatal</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">relatively</tok> <tok cat="JJ">resistant</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok></cons> <tok cat="NN">production</tok></cons></cons></cons></cons> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i3"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="CC">either</tok> <tok cat="IN">via</tok> <cons cat="NP" syn="COOD"><tok cat="PRPP">their</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">antigen</tok> <tok cat="NNS">receptors</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NNS">lectins</tok></cons></cons></cons><cons cat="ADVP" ref="i3" null="T"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@16-12-2010
942899203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">mechanism</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons> <cons cat="ADJP"><tok cat="JJ">responsible</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">phenomenon</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="ADVP"><tok cat="RB">clearly</tok></cons> <cons cat="VP"><tok cat="VBN">elucidated</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
942899204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">hypothesized</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">such</tok> <tok cat="NNS">defects</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">fetal/neonatal</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">due</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lack</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">elements</tok></cons></cons> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
942899205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">used</tok> <cons cat="NP"><tok cat="JJ">reverse</tok> <tok cat="JJ">transcriptase-polymerase</tok> <tok cat="NN">chain</tok> <tok cat="NN">reaction</tok></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">examine</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">fetal</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">term</tok> <tok cat="JJ">neonatal</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NN">cord</tok> <tok cat="NNS">bloods</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">activation</tok></cons></cons></cons><cons cat="NP" ref="i30" null="ICH"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD" id="i30"><cons cat="NP"><tok cat="NN">c-jun</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">c-fos</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">p50</tok> <tok cat="NN">subunit</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
942899206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="NNS">mRNAs</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="CD">three</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">fetal</tok> <tok cat="NN">blood</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">27th</tok> <tok cat="NN">week</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">gestation</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">term</tok> <tok cat="NN">cord</tok> <tok cat="NNS">bloods</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
942899207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">term</tok> <tok cat="NN">infant</tok> <tok cat="NN">cord</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">anti-CD3</tok> <tok cat="JJ">monoclonal</tok> <tok cat="NNS">antibodies</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">up-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-jun</tok></cons> <cons cat="NP" ref="i24" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-fos</tok> <cons cat="NP" ref="i24" null="RNR"></cons></cons><cons cat="NP" id="i24"><tok cat="NNS">mRNAs</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">15</tok> <tok cat="NN">min</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
942899208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">secretion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">anti-CD3-stimulated</tok> <tok cat="NN">cord</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">still</tok></cons> <cons cat="VP"><tok cat="VBN">blunted</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="VBN">compared</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">control</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NNS">adults</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
942899209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="JJ">fetal</tok> <tok cat="JJ">nucleated</tok> <tok cat="NN">blood</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <tok cat="VBP">express</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">important</tok> <tok cat="NNS">genes</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok></cons> <tok cat="NN">regulation</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">increase</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">accumulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NNS">mRNAs</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">anti-CD3</tok></cons> <tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
942899210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="JJ">qualitative</tok> <tok cat="NNS">differences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">capacity</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">fetal</tok> <tok cat="NN">blood</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
942899211@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Quantitative</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="VP"><tok cat="VBG">comparing</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">currently</tok></cons> <cons cat="PP" role="PRD"><tok cat="IN">under</tok> <cons cat="NP"><tok cat="NN">investigation</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
943849501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Replication</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus-1</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">autocrine</tok> <tok cat="NN">secretion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@29@@oe@16-12-2010
943849502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="LRB">(</tok><tok cat="NN">TNF</tok><tok cat="RRB">)</tok><tok cat="NN">-alpha</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">controls</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">activation</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">major</tok> <tok cat="NN">inducer</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="LRB">(</tok><tok cat="NN">HIV</tok><tok cat="RRB">)</tok><tok cat="CD">-1</tok> <tok cat="NN">replication</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="RB">chronically</tok> <tok cat="JJ">infected</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
943849503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NNS">cultures</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">HIV-infected</tok> <tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBG">neutralizing</tok> <cons cat="NP"><tok cat="JJ">anti-TNF-alpha</tok> <tok cat="NNS">antibodies</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="ADJP" null="QSTN"></cons><cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
943849504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">Primary</tok></cons> <cons cat="ADJP"><tok cat="VBG">resting</tok></cons></cons> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">produced</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">supported</tok> <cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">replication</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">receptor</tok> <tok cat="NN">activation</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
943849505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBG">neutralizing</tok> <cons cat="NP"><tok cat="JJ">anti-TNF-alpha</tok> <tok cat="NNS">antibodies</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><cons cat="ADVP"><tok cat="RB">drastically</tok></cons> <tok cat="VBD">reduced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">p24</tok> <tok cat="NN">antigen</tok></cons> <tok cat="NN">release</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">prevented</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">CD4+</tok></cons> <tok cat="NN">cell</tok> <tok cat="NN">depletion</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">infection</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
943849506@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Anti-TNF-alpha</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">prevented</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">activation</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">good</tok> <tok cat="NN">correlation</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">inhibition</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">replication</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok><cons cat="NP" ref="i8" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
943849507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" func="NOM" role="SBJ"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">supplementing</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cultures</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">doses</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">reverted</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">anti-TNF-alpha</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">proliferation</tok></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV</tok> <cons cat="NP"><tok cat="NN">p24</tok> <tok cat="NN">antigen</tok></cons> <tok cat="NN">release</tok></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activation</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NNS">cultures</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
943849508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">anti-TNF-alpha</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok></cons> <tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LTR</tok></cons><tok cat="RRB">)</tok><tok cat="JJ">-driven</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">activation</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="CC">either</tok> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">absence</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">Tat</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
943849509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">support</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">autocrine</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons> <tok cat="NN">secretion</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">controlling</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">replication</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">because</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">maintain</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">elevated</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
944054201@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="DT">A</tok> <tok cat="NN">thiol</tok> <tok cat="NN">antioxidant</tok></cons> <cons cat="VP"><tok cat="VBZ">regulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IgE</tok> <tok cat="NN">isotype</tok></cons> <tok cat="NN">switching</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inhibiting</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappaB</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@16-12-2010
944054202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="VBG">binding</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappaB</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">germline</tok> <tok cat="NN">Cepsilon</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">little</tok> <tok cat="NN">information</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">factor</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">IgE</tok></cons> <tok cat="NN">synthesis</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@41@@oe@16-12-2010
944054203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Accordingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">studied</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">germline</tok></cons> <tok cat="NN">Cepsilon</tok> <tok cat="NN">transcription</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">Burkitt</tok><tok cat="POS">'s</tok> <tok cat="NN">lymphoma</tok> <tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">DND39</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">DG75</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
944054204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">both</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">n-acetyl-L-cysteine</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NAC</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potent</tok> <tok cat="NN">thiol</tok> <tok cat="NN">antioxidant</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">triggering</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">IL-4</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">anti-CD40</tok> <tok cat="JJ">monoclonal</tok> <tok cat="NN">antibody</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
944054205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-4</tok></cons> <cons cat="VP"><tok cat="VBD">activated</tok> <cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NNS">transducers</tok> <tok cat="CC">and</tok> <tok cat="NNS">activators</tok> <tok cat="IN">of</tok> <tok cat="NN">transcription</tok> <tok cat="LRB">(</tok><tok cat="NNS">STAT</tok><tok cat="RRB">)</tok> <tok cat="CD">6</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">NAC</tok> <tok cat="NN">treatment</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transfection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">decoy</tok> <tok cat="NNS">oligodeoxynucleotides</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">STAT6</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">only</tok> <tok cat="RB">partly</tok></cons> <cons cat="VP"><tok cat="VBD">blocked</tok> <cons cat="NP"><tok cat="JJ">IL-4-induced</tok> <cons cat="NP"><tok cat="NN">germline</tok></cons> <tok cat="NN">Cepsilon</tok> <tok cat="NN">transcription</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
944054206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="CD">two</tok> <tok cat="NN">decoy</tok> <tok cat="NNS">oligodeoxynucleotides</tok></cons> <cons cat="ADVP"><tok cat="RB">together</tok></cons> <cons cat="ADVP"><tok cat="RB">almost</tok> <tok cat="RB">completely</tok></cons> <cons cat="VP"><tok cat="VBD">abrogated</tok> <cons cat="NP"><tok cat="JJ">IL-4-induced</tok> <cons cat="NP"><tok cat="NN">germline</tok></cons> <tok cat="NN">Cepsilon</tok> <tok cat="NN">transcription</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
944054207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Of</tok> <cons cat="NP"><tok cat="NN">note</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="JJ">CD40-mediated</tok> <tok cat="NN">enhancement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">IL-4-driven</tok> <cons cat="NP"><tok cat="NN">germline</tok></cons> <tok cat="NN">Cepsilon</tok> <tok cat="NN">transcription</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">markedly</tok></cons> <cons cat="VP"><tok cat="VBN">decreased</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">NAC</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">decoy</tok> <tok cat="NN">oligodeoxynucleotide</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
944054208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NAC</tok></cons></cons> <cons cat="PP" ref="i30" null="ICH"></cons></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" ref="i10" null="NONE"></cons></cons></cons><cons cat="PP" id="i30"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">deletional</tok> <tok cat="NN">switch</tok> <tok cat="NN">recombination</tok></cons> <cons cat="VP"><tok cat="VBG">underlying</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">isotype</tok> <tok cat="NN">switch</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IgE</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
944054209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NAC</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">generation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Smu/Sepsilon</tok> <tok cat="NN">switch</tok> <tok cat="NNS">fragments</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">normal</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBN">costimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-4</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">anti-CD40</tok> <tok cat="JJ">monoclonal</tok> <tok cat="NN">antibody</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
944054210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP" syn="COOD"><cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">abolished</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">IL-4-induced</tok> <tok cat="NN">upregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD40</tok></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">promoted</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">upregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD23</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
944054211@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="NN">coordination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT6</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i14" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">germline</tok></cons> <tok cat="NN">Cepsilon</tok> <tok cat="NN">transcription</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">IL-4</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">CD40-mediated</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">enhancement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">germline</tok></cons> <tok cat="NN">Cepsilon</tok> <tok cat="NN">transcription</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">class</tok> <tok cat="NN">switching</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IgE</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
944054601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">Cellular</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">molecular</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">mechanisms</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-5</tok></cons> <tok cat="NN">synthesis</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">atopic</tok> <tok cat="NNS">diseases</tok></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">study</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">allergen-specific</tok> <tok cat="JJ">human</tok> <tok cat="NN">helper</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@16-12-2010
944054602@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">BACKGROUND</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NNS">Cytokines</tok></cons> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">helper</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">intimately</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">chronic</tok> <tok cat="JJ">allergic</tok> <tok cat="NNS">diseases</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">eosinophilic</tok> <tok cat="NN">inflammation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@16-12-2010
944054603@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">OBJECTIVE</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-5</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">a</tok> <tok cat="JJ">potent</tok> <cons cat="NP"><tok cat="NN">growth</tok> <tok cat="NN">factor</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">chemotactic</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NNS">eosinophils</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">CD4+</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">asthma</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
944054604@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">METHODS</tok><tok cat="COLON">:</tok></cons> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" syn="COOD" id="i8" role="SBJ"><cons cat="NP"><tok cat="JJ">Allergen-specific</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">clones</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP" null="QSTN"></cons><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">hybridomas</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">established</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">asthma</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">responses</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NNS">stimuli</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">determined</tok><cons cat="NP" ref="i9" null="NONE"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
944054605@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">RESULTS</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">After</tok> <cons cat="NP"><tok cat="JJ">nonspecific</tok> <tok cat="NN">stimulation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">IL-5</tok></cons> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">CD4+</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">atopic</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">nonatopic</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">subjects</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">asthma</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP"><tok cat="VBN">enhanced</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="VBN">compared</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">controls</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
944054606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">atopic</tok> <tok cat="NN">asthma</tok> <tok cat="NNS">patients</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><tok cat="CC">both</tok> <cons cat="VP"><tok cat="VBD">proliferated</tok></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">produced</tok> <cons cat="NP"><tok cat="NN">IL-5</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">incubation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">mite</tok> <tok cat="NN">allergen</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="JJ">mite-specific</tok> <tok cat="NN">helper</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">eosinophilic</tok> <tok cat="NN">inflammation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">atopic</tok> <tok cat="NN">asthma</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
944054607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">human</tok> <cons cat="NP"><tok cat="NN">IL-5</tok> <tok cat="NN">promoter/enhancer</tok> <tok cat="NN">luciferase</tok> <tok cat="NN">gene</tok> <tok cat="NN">construct</tok></cons></cons> <cons cat="VP"><tok cat="VBN">transfected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="JJ">IL-5-producing</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">clones</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">clearly</tok></cons> <cons cat="VP"><tok cat="VBN">transcribed</tok> <cons cat="NP" ref="i14" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">stimulation</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">515</tok> <tok cat="NN">base</tok> <tok cat="NN">pair</tok> <cons cat="NP"><tok cat="NN">IL-5</tok> <tok cat="NN">gene</tok> <tok cat="NN">segment</tok></cons></cons> <cons cat="ADVP"><tok cat="JJ">upstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBG">coding</tok> <tok cat="NN">region</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">sufficient</tok></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">respond</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">activating</tok> <tok cat="NNS">signals</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP"><tok cat="NN">helper</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@16-12-2010
944054608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i15" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">same</tok> <tok cat="NN">gene</tok> <tok cat="NN">segment</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">transcribed</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">IL-5-nonproducing</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">clones</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i16" role="SBJ"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <cons cat="NP"><tok cat="NN">IL-5</tok></cons> <tok cat="NN">synthesis</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i16" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">level</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
944054609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Experiments</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">hybridomas</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">confirmed</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">findings</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">suggested</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">unique</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP"><tok cat="NN">IL-5</tok></cons> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
944054610@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">CONCLUSION</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" id="i17" role="SBJ"><cons cat="NP"><tok cat="VBN">Enhanced</tok> <cons cat="NP"><tok cat="NN">IL-5</tok></cons> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">helper</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">seems</tok> <cons cat="S"><cons cat="NP" ref="i17" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">cause</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">eosinophilic</tok> <tok cat="NN">inflammation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">atopic</tok></cons> <cons cat="NP" ref="i25" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">nonatopic</tok></cons> <cons cat="NP" ref="i25" null="RNR"></cons></cons><cons cat="NP" id="i25"><tok cat="NN">asthma</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
944054611@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Elucidation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">IL-5-specific</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">mechanisms</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">facilitate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">development</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">novel</tok> <tok cat="NNS">treatments</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">allergic</tok> <tok cat="NNS">diseases</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">eosinophilic</tok> <tok cat="NN">inflammation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
944237301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">beta</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">stability</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@16-12-2010
944237302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">Transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="VP"><tok cat="MD">must</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">released</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cytoplasmic</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NNS">molecules</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NNS">Bs</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">in</tok> <tok cat="NN">order</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">move</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <cons cat="NP"><tok cat="NN">target</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@30@@oe@16-12-2010
944237303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="JJ">Little</tok> <cons cat="PP" ref="i30" null="ICH"></cons></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="NP" ref="i6" null="NONE"></cons></cons></cons><cons cat="PP" id="i30"><tok cat="IN">about</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">maintenance</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">constitutive</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">some</tok> <tok cat="NNS">cell-types</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">sustained</tok> <tok cat="JJ">nuclear</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
944237304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="VBN">Increased</tok> <tok cat="NN">turnover</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">constitutive</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
944237305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons> <cons cat="VP"><tok cat="VBD">compared</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">turnover</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">beta</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HeLa</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
944237306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">Both</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBP">display</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">high</tok> <tok cat="NN">turnover</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">beta</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><cons cat="ADVP"><tok cat="RB">considerably</tok> <tok cat="RBR">more</tok></cons> <tok cat="JJ">stable</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
944237307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">half-life</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">inhibitors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">increased</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">HeLa</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
944237308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="JJ">other</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B/I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NNS">molecules</tok></cons> <cons cat="VP"><tok cat="VBN">tested</tok> <cons cat="NP" null="NONE"></cons></cons></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">relatively</tok> <tok cat="JJ">stable</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">cell-types</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
944237309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">elevated</tok> <tok cat="NN">turnover</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">endogenous</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Namalwa</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">a</tok> <tok cat="NN">proteasome</tok> <tok cat="NN">inhibitor</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="VP"><tok cat="VBZ">seems</tok> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">driven</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NN">degradation</tok> <tok cat="NN">machinery</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJR">slower</tok> <tok cat="NN">turnover</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">non-B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
944237310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">processes</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">persistent</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
944237311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">signaling</tok></cons> <cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">rapid</tok> <tok cat="NN">depletion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">beta</tok></cons> <tok cat="NNS">pools</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
944237312@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">efficiently</tok></cons> <cons cat="VP"><tok cat="VBN">resynthesized</tok> <cons cat="NP" ref="i11" null="NONE"></cons></cons><cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">beta</tok></cons> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBP">stay</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">low</tok> <cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">prolonged</tok> <tok cat="NN">time</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
944237313@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NNS">hours</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
944237314@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">removal</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons> <tok cat="NN">containing</tok> <tok cat="NN">medium</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">causes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">rapid</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
944237315@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">phosphoform</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">newly</tok> <tok cat="VBN">synthesized</tok></cons> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">visible</tok></cons> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i13"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="NN">degradation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">proteasome</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i14" null="NONE"></cons><cons cat="ADVP" ref="i13" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">new</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">displays</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NNS">properties</tok></cons> <cons cat="PP"><tok cat="VBG">regarding</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">degradation</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">second</tok> <tok cat="NN">inducer</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
944237316@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">There</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">no</tok> <tok cat="JJ">significant</tok> <tok cat="NN">difference</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">turnover</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">pre-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">post-inductive</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
944237317@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">observations</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">resynthesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">removal</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">stimulus</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><tok cat="JJ">obligatory</tok> <tok cat="NNS">steps</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">inactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
944237401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">beta</tok> <tok cat="NN">inhibitor</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
944237402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i15" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="JJ">maintained</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">inactive</tok></cons> <cons cat="ADJP"><tok cat="JJ">cytoplasmic</tok></cons></cons> <tok cat="NN">state</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NNS">inhibitors</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@16-12-2010
944237403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">mammalian</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i6" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">beta</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">purified</tok> <cons cat="NP" ref="i6" null="NONE"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">inhibitors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">association</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">p65</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">c-Rel</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NNS">subunits</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
944237404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">third</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">inhibitor</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">epsilon</tok></cons> <tok cat="LRB">(</tok><tok cat="CD">1</tok><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
944237405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">large</tok> <tok cat="NN">variety</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">inducers</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i18" role="SBJ"><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">beta</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">proteolytically</tok></cons> <cons cat="VP"><tok cat="VBN">degraded</tok><cons cat="NP" ref="i18" null="NONE"></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
944237406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">E29.1</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">hybridoma</tok></cons></cons> <cons cat="NP" syn="COOD" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">beta</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">equally</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">beta</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">degraded</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">TNF</tok> <tok cat="NN">alpha</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">contrast</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="SBAR" func="NOM"><cons cat="WHNP" id="i10"><tok cat="WP">what</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="ADVP"><tok cat="RB">originally</tok></cons> <cons cat="VP"><tok cat="VBN">published</tok><cons cat="NP" ref="i10" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
944237407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">data</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok><cons cat="S"><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">unlike</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i12" role="SBJ"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">beta</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP" syn="COOD"><cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <cons cat="VP"><tok cat="VBN">phosphorylated</tok> <cons cat="NP" ref="i12" null="NONE"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">resynthesized</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="VBN">hypophosphorylated</tok> <tok cat="NN">form</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
944237408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">absence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">slow</tok> <tok cat="JJ">migrating</tok></cons> <tok cat="NNS">forms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">beta</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBG">following</tok> <cons cat="NP"><tok cat="NN">stimulation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="ADVP"><tok cat="RB">necessarily</tok></cons> <cons cat="VP"><tok cat="VB">constitute</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">signal-induced</tok> <tok cat="NN">event</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">targets</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">molecule</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">proteolysis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
944237701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B/Rel</tok> <tok cat="NN">family</tok> <tok cat="NNS">members</tok></cons> <cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">ICAM-1</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NN">THP-1</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
944237702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">A</tok> <cons cat="NP"><tok cat="NN">kappa</tok> <tok cat="NN">B-site</tok></cons> <cons cat="SBAR" ref="i25" null="ICH"></cons></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">promoter</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">intercellular</tok> <tok cat="NN">adhesion</tok> <tok cat="NN">molecule-1</tok> <tok cat="LRB">(</tok><tok cat="NN">ICAM-1</tok><tok cat="RRB">)</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" id="i25"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" id="i15" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">ICAM-1</tok></cons> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@37@@oe@16-12-2010
944237703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">bind</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">transactivate</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="DT">this</tok> <cons cat="NP"><tok cat="NN">enhancer</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
944237704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="FW">In</tok> <tok cat="FW">vitro</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">ICAM-1</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="VP"><tok cat="VBD">appeared</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">bind</tok> <cons cat="NP" syn="COOD" id="i30"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">RelA</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Rel</tok> <tok cat="NNS">homodimers</tok></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NNS">heterodimers</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B1</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">weakly</tok></cons> <cons map="i30" cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B1</tok> <tok cat="NNS">homodimers</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
944237705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i9" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">RelA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Rel</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B1</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">transactivate</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">ICAM-1</tok> <tok cat="NN">kappa</tok> <tok cat="JJ">B-reporter</tok> <tok cat="NN">construct</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
944237706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NN">THP-1</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="NN">TNF-alpha</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">complexes</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <cons cat="VP"><tok cat="VBD">bound</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">ICAM-1</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
944237707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><tok cat="NNS">antibodies</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">supershift</tok> <tok cat="NN">assay</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">complexes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">contain</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">RelA</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJR">higher</tok> <tok cat="NN">affinity</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">consensus</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
944237708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">second</tok> <tok cat="NN">complex</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP"><tok cat="NN">RelA</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">exhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJR">higher</tok> <tok cat="NN">affinity</tok></cons> <cons cat="PP"><tok cat="IN">towards</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">ICAM-1</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
944237709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">repress</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">RelA</tok> <tok cat="NN">homodimer</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B1/RelA</tok> <tok cat="NN">heterodimer</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
944237710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">argue</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="NN">RelA</tok> <tok cat="NNS">homodimers</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">likely</tok> <cons cat="S"><cons cat="NP" ref="i14" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">dominant</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">TNF-alpha-induced</tok> <cons cat="NP"><tok cat="NN">ICAM-1</tok></cons> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
944238001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">LPS</tok> <tok cat="NN">tolerance</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">monocytes/macrophages</tok></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="CD">three</tok> <tok cat="JJ">3'</tok> <tok cat="NNS">cytosins</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">motif</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">detection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">upregulated</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">p50</tok> <tok cat="NNS">homodimers</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@16-12-2010
944238002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i4"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" id="i25" role="SBJ"><tok cat="NNS">monocytes</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" ref="i25" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">LPS</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">lipopolysaccharide</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">repeatedly</tok></cons> <cons cat="ADVP" ref="i4" null="T"></cons></cons></cons></cons></cons><cons cat="S"><cons cat="ADVP"><tok cat="RB">then</tok></cons> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">initially</tok> <tok cat="JJ">high</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TNF</tok> <tok cat="LRB">(</tok><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok><tok cat="RRB">)</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <tok cat="RB">very</tok> <tok cat="JJ">low</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="FW">i.e.</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">tolerant</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">LPS</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@37@@oe@16-12-2010
944238003@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Tolerant</tok> <tok cat="NNS">cells</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">still</tok></cons> <cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CD14</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">still</tok></cons> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i6" null="NONE"></cons></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">mobilize</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="NN">nucleus</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
944238004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBG">binding</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBG">employing</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">-605</tok> <tok cat="NN">motif</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">TNF</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GGGGCTGTCCC</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">tolerant</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">monocytic</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons> <cons cat="NP"><tok cat="NN">Mono</tok> <tok cat="NN">Mac</tok> <tok cat="CD">6</tok></cons></cons></cons></cons></cons> <cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">predominance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">p50p50</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
944238005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">mutant</tok> <tok cat="NN">motif</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">exchanges</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">terminal</tok> <tok cat="JJ">3'</tok> <tok cat="NN">C</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">G</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">fails</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">p50</tok> <tok cat="NN">homodimer</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" id="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">upregulated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">LPS</tok> <tok cat="NN">toler</tok> <tok cat="JJ">ant</tok></cons> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">Mono</tok> <tok cat="NN">Mac</tok> <tok cat="CD">6</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
944238006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">same</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">true</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" id="i12"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="VP"><tok cat="VBN">taken</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">P388D1</tok> <tok cat="NN">macrophage</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i11"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">tested</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="CD">-516</tok> <tok cat="NN">motif</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">TNF</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GGGGGCTTTCCC</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><cons cat="ADVP" ref="i11" null="T"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
944238007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">wild</tok> <tok cat="NN">type</tok> <tok cat="NN">motif</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">gives</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">efficient</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">p50p50</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i13"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i13" id="i14" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">again</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">upregulated</tok> <cons cat="NP" ref="i14" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">tolerant</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">mutant</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">3'</tok> <tok cat="NN">G</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">shows</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">hardly</tok> <tok cat="DT">any</tok></cons> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p50p50</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
944238008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Conversely</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">kappa</tok> <tok cat="JJ">light</tok> <tok cat="NN">chain</tok> <tok cat="NN">enhancer</tok> <tok cat="NN">motif</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GGGGACTTTCCG</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="ADVP"><tok cat="RB">efficiently</tok></cons> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <cons cat="NP"><tok cat="NN">p50p50</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">tolerant</tok> <tok cat="JJ">murine</tok> <tok cat="NN">P388</tok> <tok cat="NNS">macrophages</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
944238009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i15" role="SBJ"><tok cat="NN">Binding</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">readily</tok></cons> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i15" null="NONE"></cons></cons><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i17"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i16" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">3'</tok> <tok cat="NN">G</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">replaced</tok> <cons cat="NP" ref="i16" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">a</tok> <tok cat="NN">C</tok></cons></cons><cons cat="ADVP" ref="i17" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
944238010@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">detection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">upregulated</tok> <cons cat="NP"><tok cat="NN">p50</tok> <tok cat="NNS">homodimers</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">LPS</tok> <cons cat="NP"><tok cat="JJ">tolerant</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">subtle</tok> <tok cat="NNS">differences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">sequence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">motif</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
944238701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">winged-helix</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="NP"><tok cat="NN">Trident</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="RB">actively</tok> <cons cat="NP"><tok cat="VBG">dividing</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@12@@oe@16-12-2010
944238702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">winged-helix</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="NP"><tok cat="NN">Trident</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">described</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">expression</tok> <tok cat="NN">pattern</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">synchronized</tok> <tok cat="NNS">fibroblasts</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@16-12-2010
944238703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="VP"><tok cat="VBN">studied</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Trident</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="VBG">differentiating</tok> <tok cat="NNS">thymocytes</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
944238704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">During</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">differentiation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">peaked</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="RB">actively</tok> <tok cat="VBG">dividing</tok> <tok cat="JJ">immature</tok> <tok cat="JJ">single</tok> <tok cat="JJ">positive</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
944238705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">lymphocytes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Trident</tok> <tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">absent</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NNS">mitogens</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
944238706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">observations</tok></cons> <cons cat="VP"><tok cat="VBP">imply</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Trident</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBG">dividing</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
944238801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Inefficient</tok> <tok cat="NN">termination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">antigen</tok> <tok cat="NNS">responses</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">NF-ATp-deficient</tok> <tok cat="NNS">mice</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@9@@oe@16-12-2010
944238802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">In</tok> <tok cat="NN">order</tok> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">elucidate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-ATp</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RBS">most</tok> <tok cat="JJ">prominent</tok></cons> <tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">family</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <cons cat="NP"><tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">created</tok> <cons cat="NP"><tok cat="JJ">NF-ATp-deficient</tok> <tok cat="NNS">mice</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="VBG">targeting</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@40@@oe@16-12-2010
944238803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="JJ">Such</tok> <tok cat="JJ">NF-ATp-/-</tok> <tok cat="NNS">mice</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">born</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">appear</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">develop</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">normal</tok> <tok cat="JJ">immune</tok> <tok cat="NN">system</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
944238804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Apart</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">clear-cut</tok> <tok cat="NNS">defects</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">synthesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">mRNAs</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Th2-type</tok> <tok cat="NNS">lymphokines</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-4</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-5</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-13</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">primary</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">secondary</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">stimulations</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">spleen</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">distinct</tok></cons> <cons cat="ADJP"><tok cat="JJ">impaired</tok></cons></cons> <tok cat="NN">deletion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">V</tok> <tok cat="NN">beta</tok> <tok cat="JJ">11+/CD4+</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">mice</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">superantigen</tok> <tok cat="NN">injection</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@54@@oe@16-12-2010
944238805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">NF-ATp-/-</tok> <tok cat="NNS">mice</tok></cons> <cons cat="ADJP"><tok cat="JJR">older</tok> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><tok cat="CD">6</tok> <tok cat="NNS">weeks</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP"><tok cat="CD">2-5</tok> <tok cat="JJ">fold</tok></cons> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
944238806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">correlated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="VBN">increased</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok> <tok cat="NNS">markers</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CD44</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CD69</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">decreased</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD62</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
944239301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Identification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">target</tok> <tok cat="NNS">genes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">lymphoid-specific</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">Oct2</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@11@@oe@16-12-2010
944239302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">Oct2</tok></cons> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><cons cat="ADVP"><tok cat="RB">predominantly</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">essential</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">phase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@16-12-2010
944239303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">regulatory</tok> <tok cat="NNS">regions</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">contain</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="VBG">binding</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">Oct2</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
944239304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Nevertheless</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">none</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">originally</tok></cons> <tok cat="VBN">thought</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">Oct2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">affected</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">Oct2-deficient</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
944239305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">attempt</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">find</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">such</tok> <tok cat="JJ">elusive</tok></cons> <cons cat="NP"><tok cat="NN">Oct2</tok></cons> <cons cat="NP"><tok cat="NN">target</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">understand</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Oct2</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">development</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">isolated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cDNAs</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Oct2</tok></cons> <cons cat="NP"><tok cat="NN">target</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
944239306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">So</tok> <tok cat="RB">far</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">five</tok> <tok cat="JJ">potential</tok> <tok cat="NNS">targets</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">Oct2</tok></cons></cons><cons cat="NP" ref="i30" null="ICH"></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD" id="i30"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">membrane</tok> <tok cat="NN">glycoprotein</tok></cons></cons> <cons cat="NP"><tok cat="NN">CD36</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cysteine-rich</tok> <tok cat="VBN">secreted</tok> <tok cat="NN">protein</tok> <tok cat="CD">3</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CRISP-3</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">mouse</tok> <tok cat="NN">homolog</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">monocyte/neutrophil</tok> <tok cat="NN">elastase</tok> <tok cat="NN">inhibitor</tok></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">mEI</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">unknown</tok> <cons cat="NP"><tok cat="NN">cDNA</tok> <tok cat="NNS">sequences</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Nov1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Nov2</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@16-12-2010
944239307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <cons cat="NP"><tok cat="NN">target</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">quite</tok> <tok cat="JJ">distinct</tok></cons> <tok cat="NN">expression</tok> <tok cat="NNS">patterns</tok></cons> <cons cat="VP"><tok cat="VBG">demonstrating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i15" role="SBJ"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">Oct2</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">regulation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
944239308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Whereas</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">CD36</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">mEI</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">all</tok> <cons cat="NP"><tok cat="JJ">hematopoetic</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="NN">Oct2</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok><tok cat="PERIOD">.</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">CRISP-3</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">pre-B</tok> <tok cat="JJ">cell-specific</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Nov1</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="NN">plasma</tok> <tok cat="NN">B</tok> <tok cat="JJ">cell-specific</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="NN">Nov2</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="NN">B</tok> <tok cat="RB">cell-specifically</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok><cons cat="NP" ref="i9" null="NONE"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
944239501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Temporal</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">IgH</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBG">developing</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">3'</tok> <tok cat="NN">locus</tok> <tok cat="NN">control</tok> <tok cat="NN">region</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@16-12-2010
944239502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="VBN">suggested</tok> <tok cat="NNS">roles</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">downstream</tok> <tok cat="JJ">3'</tok> <tok cat="NNS">regions</tok></cons></cons> <cons cat="VP"><tok cat="VBG">acting</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Locus</tok> <tok cat="NN">Control</tok> <tok cat="NN">Region</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LCR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">allowed</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NNS">comparisons</tok> <cons cat="PP" ref="i30" null="ICH"></cons><cons cat="PP" ref="i31" null="ICH"></cons></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">made</tok> <cons cat="NP" ref="i5" null="NONE"></cons></cons></cons></cons></cons></cons></cons><cons cat="PP" id="i30"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">IgH</tok> <tok cat="NN">locus</tok></cons></cons></cons></cons></cons> <cons cat="PP" id="i31"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NN">model</tok> <tok cat="NNS">systems</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WPP">whose</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" id="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">governed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">LCR</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@44@@oe@16-12-2010
944239503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">summarize</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">importance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">IgH</tok> <tok cat="NN">3'LCR</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">putative</tok> <tok cat="JJ">functional</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">IgH</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">compare</tok> <cons cat="NP"><tok cat="PRP">it</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">5'LCR</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">beta-globin</tok> <tok cat="NN">locus</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
944240001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">influenza</tok> <tok cat="NN">A</tok> <tok cat="NN">virus</tok> <tok cat="NN">infection</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@11@@oe@16-12-2010
944240002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">influenza</tok> <tok cat="NN">A</tok> <tok cat="NN">virus</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">broad</tok> <tok cat="NN">range</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">proinflammatory</tok> <tok cat="NNS">cytokines</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NN">cell</tok></cons> <cons cat="VP"><tok cat="NN">attracting</tok> <cons cat="NP"><tok cat="NNS">chemokines</tok></cons></cons></cons></cons></cons></cons> <cons cat="SBAR" func="ADV" adv="TMP"><tok cat="IN">before</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">infected</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBP">undergo</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@16-12-2010
944240003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="VBG">underlying</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">corresponding</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">transcriptionally</tok></cons> <cons cat="VP"><tok cat="VBN">initiated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">virus</tok> <tok cat="NN">infection</tok></cons></cons> <cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">still</tok></cons> <cons cat="ADVP"><tok cat="RB">poorly</tok></cons> <cons cat="VP"><tok cat="VBN">understood</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
944240004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">seems</tok> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">many</tok> <cons cat="NP"><tok cat="JJ">proinflammatory</tok> <tok cat="NN">cytokine</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="MD">can</tok><tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><tok cat="DT">the</tok> <tok cat="JJ">only</tok> <tok cat="NN">mechanism</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">since</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">several</tok> <cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="VP"><tok cat="VBP">lack</tok> <cons cat="NP"><tok cat="JJ">respective</tok> <cons cat="NP"><tok cat="VBG">binding</tok> <tok cat="NNS">sites</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NNS">regions</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
944240005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="ADVP"><tok cat="RB">additionally</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">possible</tok> <tok cat="NN">importance</tok></cons></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CREB</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CTF</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">OTF-1</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">OTF-2</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
944240006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">explore</tok> <cons cat="NP"><tok cat="JJ">long-term</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">mechanisms</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok> <tok cat="NN">level</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">substitute</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="VBN">metabolized</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
944240007@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">cell-type-specific</tok></cons> <cons cat="ADJP"><tok cat="JJ">differential</tok></cons></cons> <tok cat="NN">response</tok></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">CREB</tok><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CTF</tok><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">OTF-1</tok><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">OFT-2</tok><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">genes</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP" adv="TMP"><cons cat="NP"><cons cat="QP"><tok cat="CD">1</tok> <tok cat="TO">to</tok> <tok cat="CD">4</tok></cons> <tok cat="NNS">hours</tok></cons> <tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">influenza</tok> <tok cat="NN">A</tok> <tok cat="NN">virus</tok> <tok cat="NN">infection</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons> <cons cat="NP"><tok cat="NN">Mono</tok> <tok cat="NN">Mac</tok> <tok cat="CD">6</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">freshly</tok> <tok cat="VBN">prepared</tok></cons> <tok cat="JJ">human</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons> <cons cat="NP" id="i10" role="SBJ"><tok cat="DT">no</tok> <tok cat="JJ">significant</tok> <tok cat="NNS">changes</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">detected</tok><cons cat="NP" ref="i10" null="NONE"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@52@@oe@16-12-2010
944240008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">infected</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i11"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">die</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CREB</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CTF</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">OTF-2</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">rather</tok></cons> <cons cat="VP"><tok cat="VBN">suppressed</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP" adv="TMP"><cons cat="NP"><tok cat="CD">8</tok> <tok cat="NNS">hours</tok></cons> <tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">infection</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
944240009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">conclusion</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i13" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">long-term</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">non-proliferating</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">seems</tok> <cons cat="S"><cons cat="NP" ref="i13" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="PP" role="PRD"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">minor</tok> <tok cat="NN">importance</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">influenza</tok> <tok cat="NN">infection</tok></cons></cons></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">since</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">apoptosisprone</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">immediate</tok> <tok cat="NN">availability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NNS">proteins</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok><cons cat="NP" ref="i14" null="NONE"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
944632201@GENIA Treebank@formal@@1@S@<cons cat="PRN"><cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP"><cons cat="NP"><tok cat="JJ">Molecular-biologic</tok> <tok cat="NNS">aspects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">nervous</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">immune</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">systems</tok></cons></cons></cons></cons></cons></cons><tok cat="RRB">]</tok></cons></cons>@@@@1@11@@oe@16-12-2010
944632202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">problem</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">neuro-immuno</tok> <tok cat="NNS">interactions</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NNS">trans-factors</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">stimulating</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-2</tok> <tok cat="LRB">(</tok><tok cat="NN">IL-2</tok><tok cat="RRB">)</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">discussed</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@16-12-2010
944632203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="DT">The</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">physico-chemical</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">functional</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">parameters</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">low</tok> <tok cat="JJ">molecular</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">SP</tok></cons> <tok cat="CC">and</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">BP- 14</tok><cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><tok cat="CD">18</tok><cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><tok cat="CD">19</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NNS">kDs</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">splenic</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">brain</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">immunized</tok> <tok cat="NNS">rats</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">studied</tok><cons cat="NP" ref="i6" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
944632204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i8" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2mRNA</tok></cons> <tok cat="NN">synthesis</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">splenic</tok> <tok cat="NN">T-</tok><tok cat="NNS">lymphocytes</tok> <tok cat="NN">culture</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NNS">conditions</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">shown</tok><cons cat="NP" ref="i8" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
944632205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">protective</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">SP</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">BP</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2mRNA</tok></cons> <tok cat="NN">synthesis</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">stressful</tok> <tok cat="NNS">conditions</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NNS">T-cells</tok></cons> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CsA</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok><cons cat="NP" ref="i9" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
946483601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-12</tok></cons> <cons cat="NP"><tok cat="NN">p40</tok> <tok cat="NN">transcript</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="NN">CD40</tok></cons> <tok cat="NN">ligation</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappaB</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@16-12-2010
946483602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">Interleukin-12</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">bacteria</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NNS">parasites</tok></cons></cons></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">bacterial</tok> <tok cat="NNS">constituents</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">LPS</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">monocytes/macrophages</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">dendritic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">generated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">antigen-presenting</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD40-CD40</tok> <tok cat="NN">ligand</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CD40L</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@44@@oe@16-12-2010
946483603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">So</tok> <tok cat="RB">far</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NNS">analyses</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p40</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">carried</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PRT"><tok cat="RP">out</tok></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">only</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">bacterial</tok> <tok cat="NNS">constituents</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">LPS</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="NNS">stimuli</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
946483604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">characterized</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p40</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="NN">CD40</tok></cons> <tok cat="NN">ligation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="JJ">lymphoblastoid</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Daudi</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">acute</tok> <tok cat="JJ">monocytic</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">THP-1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
946483605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">These</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">agonistic</tok> <tok cat="JJ">monoclonal</tok> <tok cat="NN">antibody</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="NN">CD40</tok></cons></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">transfection</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">CD40L</tok> <tok cat="NN">expression</tok> <tok cat="NN">vector</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">secreted</tok> <cons cat="NP"><tok cat="NN">p40</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><tok cat="VBN">enhanced</tok> <cons cat="NP"><tok cat="NN">p40</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
946483606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Sequence</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">p40</tok> <tok cat="NN">promoter</tok> <tok cat="NN">region</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <cons cat="NP"><tok cat="JJ">potential</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="LRB">(</tok><tok cat="NN">NF</tok><tok cat="RRB">)</tok><tok cat="NN">-kappaB</tok> <tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons></cons> <cons cat="VP"><tok cat="VBN">conserved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">mouse</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">human</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
946483607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">potential</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">binding</tok> <tok cat="NN">sequence</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">around</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">120</tok> <tok cat="NN">bp</tok> <tok cat="RB">upstream</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">initiation</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">murine</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">p40</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">formed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">complex</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">extract</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Daudi</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">CD40</tok></cons> <tok cat="NN">ligation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
946483608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">transfection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Daudi</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">polymerized</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">binding</tok> <tok cat="NN">sequence</tok></cons></cons> <cons cat="VP"><tok cat="VBN">ligated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">thymidine</tok> <tok cat="NN">kinase/chloramphenicol</tok> <tok cat="NN">acetyltransferase</tok> <tok cat="LRB">(</tok><tok cat="NN">CAT</tok><tok cat="RRB">)</tok> <tok cat="NN">reporter</tok> <tok cat="NN">plasmid</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">greatly</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CAT</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">transfection</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">polymerized</tok> <tok cat="VBN">mutated</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">binding</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok><cons cat="VP" null="QSTN"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
946483609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">binding</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">around</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">120</tok> <tok cat="NN">bp</tok> <tok cat="RB">upstream</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">initiation</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">murine</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><cons cat="NP"><tok cat="NN">p40</tok></cons> <tok cat="NN">promoter</tok> <tok cat="NNS">regions</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">p40</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="NN">CD40</tok></cons> <tok cat="NN">ligation</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
946737601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">subunit</tok> <tok cat="NN">p65</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">accumulation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">lipopolysaccharide-stimulated</tok> <tok cat="JJ">human</tok> <tok cat="JJ">monocytic</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">sodium</tok> <tok cat="NN">salicylate</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
946737602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Lipopolysaccharide</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RBS">most</tok> <tok cat="JJ">potent</tok></cons> <tok cat="NN">trigger</tok> <tok cat="NNS">substances</tok></cons> <cons cat="VP" ref="i1" null="ICH"></cons></cons></cons></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons></cons></cons> <cons cat="VP" id="i1"><tok cat="VBG">causing</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">secretion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NNS">mediators</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">interleukin-1</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
946737603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">nature</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">factors</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NN">cytokine</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">still</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">unknown</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
946737604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="JJ">Nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons><tok cat="COLON">;</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">heterodimer</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p50</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons></cons></cons></cons><tok cat="RRB">)</tok></cons> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">suggested</tok> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NNS">mediators</tok></cons></cons></cons></cons> <cons cat="SBAR" adv="TMP"><tok cat="WRB">when</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="NNS">monocytes</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">lipopolysaccharide</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
946737605@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="JJ">Nonsteroidal</tok> <tok cat="JJ">anti-inflammatory</tok> <tok cat="NNS">drugs</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><tok cat="NNS">salicylates</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">treat</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">symptoms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">inflammation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">new</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">drug</tok> <tok cat="NN">action</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">suggested</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">recently</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
946737606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NNS">Salicylates</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><tok cat="JJ">lipopolysaccharide-induced</tok> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><cons cat="VP"><tok cat="VBG">preventing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">degradation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="NP"><tok cat="LQT">"</tok><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok><tok cat="RQT">"</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">blocking</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">compartment</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
946737607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nature</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">subunit</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">mechanism</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">defined</tok><cons cat="NP" ref="i7" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
946737608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">examine</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i8"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">salicylates</tok></cons> <cons cat="VP"><tok cat="VBP">affect</tok> <cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons><cons cat="PP" ref="i8" null="T"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">amount</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">active</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">inactive</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Porphyromonas</tok> <tok cat="NN">gingivalis</tok> <tok cat="JJ">lipopolysaccharide-stimulated</tok> <tok cat="JJ">human</tok> <tok cat="JJ">monocytic</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">assessed</tok><cons cat="NP" ref="i9" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
946737609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">High</tok> <tok cat="NNS">doses</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">sodium</tok> <tok cat="NN">salicylate</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">suppressed</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">p65</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">accumulation</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">suppression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">total</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">p50</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">tissue</tok> <tok cat="NN">oligonucleotide</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">lipopolysaccharide-induced</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
946737610@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">p65</tok> <tok cat="NN">subunit</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">salicylate</tok> <tok cat="NN">treatment</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">highlight</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">salicylate</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NNS">mediators</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
947531501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cord</tok> <tok cat="NN">blood</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">neonates</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">preterms</tok></cons> <cons cat="VP"><tok cat="VBG">suffering</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">respiratory</tok> <tok cat="NN">distress</tok> <tok cat="NN">syndrome</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@16-12-2010
947531502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">measured</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GR</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">cord</tok> <tok cat="NN">blood</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok> <tok cat="NN">affinity</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Kd</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="CD">15</tok> <tok cat="NN">term</tok> <cons cat="NP" null="QSTN"></cons></cons></cons><tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="CD">20</tok> <tok cat="JJ">preterm</tok> <tok cat="NNS">babies</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@30@@oe@16-12-2010
947531503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">Thirteen</tok> <tok cat="NNS">preterms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">latter</tok> <tok cat="NN">group</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">received</tok> <cons cat="NP"><tok cat="JJ">prenatal</tok> <tok cat="NN">steroid</tok> <tok cat="NN">treatment</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
947531504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="CD">Seven</tok> <tok cat="NNS">preterms</tok></cons> <cons cat="VP"><tok cat="VBD">developed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">neonatal</tok> <tok cat="JJ">respiratory</tok> <tok cat="NN">distress</tok> <tok cat="NN">syndrome</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NRDS</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
947531505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GR</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Kd</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">similar</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">term</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">preterm</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NN">NRDS</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" id="i23"><tok cat="NNS">babies</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
947531506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">maximum</tok> <tok cat="NN">(3H)-thymidine</tok> <tok cat="NN">incorporation</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">DNA</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cord</tok> <tok cat="NN">blood</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="NNS">preterms</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" ref="i24" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP" ref="i24" null="RNR"></cons></cons><cons cat="NP" id="i24"><tok cat="NN">NRDS</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">suppressed</tok> <cons cat="NP" ref="i5" null="NONE"></cons></cons><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i6"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">term</tok> <tok cat="NNS">babies</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NNS">adults</tok></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i6" null="T"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
947531507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="ADVP"><tok cat="RB">partly</tok></cons> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">explained</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="JJ">antenatal</tok> <tok cat="NN">steroid</tok> <tok cat="NN">treatment</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
947531508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Sensitivity</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ID50</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">dexamethasone</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><tok cat="DT">the</tok> <tok cat="JJ">same</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">all</tok> <tok cat="NNS">groups</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
947531509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">number</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">function</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GR</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unable</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">find</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">relation</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">functionality</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GR</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">development</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NRDS</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
947962801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">Oxidants</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">intestinal</tok> <tok cat="NN">inflammation</tok></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@9@@oe@16-12-2010
947962802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="ADVP"><tok cat="RB">well</tok></cons> <cons cat="VP"><tok cat="VBN">appreciated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="JJ">chronic</tok> <tok cat="NN">gut</tok> <tok cat="NN">inflammation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">characterized</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="VBN">enhanced</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">reactive</tok> <tok cat="NNS">metabolites</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">oxygen</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">nitrogen</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@22@@oe@16-12-2010
947962803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">Some</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">oxidants</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">modulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">variety</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" id="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">immune</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">inflammatory</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">responses</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
947962804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">For</tok> <cons cat="NP"><tok cat="NN">example</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><tok cat="JJ">certain</tok> <tok cat="NNS">oxidants</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">regulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">variety</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">different</tok> <cons cat="NP"><tok cat="NN">adhesion</tok> <tok cat="NNS">molecules</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">enzymes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
947962805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="NNS">Oxidants</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">another</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">activator</tok> <tok cat="NN">protein-1</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
947962806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="DT">This</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">composed</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">products</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">fos</tok> <tok cat="CC">and</tok> <tok cat="NN">jun</tok> <tok cat="NN">proto-oncogene</tok> <tok cat="NN">family</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">believed</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP" syn="COOD"><tok cat="NN">cell</tok> <cons cat="NP"><tok cat="NN">growth</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">proliferation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
947962807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="NNS">oxidants</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">believed</tok> <cons cat="S"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">promote</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">intestinal</tok> <tok cat="JJ">epithelial</tok> <tok cat="NN">cell</tok></cons> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i13" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">lymphoma/leukemia-2</tok> <tok cat="NN">gene</tok> <tok cat="NN">product</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">believed</tok> <cons cat="S"><cons cat="NP" ref="i13" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">phenomenon</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">antioxidant-dependent</tok> <tok cat="NN">manner</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
947962808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">Taken</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">together</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">observations</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">nontoxic</tok> <tok cat="NNS">concentrations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">reactive</tok> <tok cat="NNS">metabolites</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">oxygen</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">nitrogen</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inflammatory</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">modulating</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
948370501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">fibromyalgia</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">low</tok> <tok cat="JJ">back</tok> <tok cat="NN">pain</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@9@@oe@16-12-2010
948370502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Recently</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">fibromyalgia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">FMS</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">disorder</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">altered</tok> <tok cat="NN">functioning</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">stress</tok> <tok cat="NN">response</tok> <tok cat="NN">system</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@16-12-2010
948370503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">FMS</tok> <tok cat="NNS">patients</tok></cons> <cons cat="VP"><tok cat="VBP">display</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">hyperreactive</tok></cons> <cons cat="ADJP"><tok cat="JJ">pituitary</tok></cons></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">adrenocorticotropic</tok> <tok cat="NN">hormone</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ACTH</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">release</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">corticotropin-releasing</tok> <tok cat="NN">hormone</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CRH</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">insulin-induced</tok> <tok cat="NN">hypoglycemia</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
948370504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">suggested</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="JJ">negative</tok> <tok cat="NN">feedback</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cortisol</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">deranged</tok><cons cat="NP" ref="i6" null="NONE"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
948370505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NNS">properties</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">function</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GR</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">FMS</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">compared</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">results</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">persons</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">chronic</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">low</tok> <tok cat="JJ">back</tok> <tok cat="NN">pain</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">LBP</tok></cons> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">localized</tok> <tok cat="NN">pain</tok> <tok cat="NN">condition</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
948370506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i8" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="CD">Forty</tok> <tok cat="JJ">primary</tok> <tok cat="NN">FMS</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">F</tok><tok cat="COLON">:</tok><tok cat="NN">M</tok></cons> <cons cat="ADJP"><tok cat="JJ">=</tok> <cons cat="NP"><tok cat="CD">36</tok><tok cat="COLON">:</tok><tok cat="CD">4</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">28</tok> <tok cat="NN">LBP</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">25</tok><tok cat="COLON">:</tok><tok cat="CD">3</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="QP"><tok cat="CD">14</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">12</tok><tok cat="COLON">:</tok><tok cat="CD">2</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">healthy</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">sedentary</tok></cons></cons> <tok cat="JJ">control</tok> <tok cat="NNS">persons</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">recruited</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">study</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
948370507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Urinary</tok> <tok cat="JJ">free</tok> <tok cat="NN">cortisol</tok> <tok cat="NN">excretion</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">FMS</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">LBP</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NNS">patients</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJR">lower</tok> <cons cat="PP"><tok cat="VBN">compared</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NNS">controls</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
948370508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="RB">Only</tok> <tok cat="NN">FMS</tok> <tok cat="NNS">patients</tok></cons> <cons cat="VP"><tok cat="VBD">displayed</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJR">lower</tok> <tok cat="NN">CBG</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">basal</tok> <tok cat="NN">serum</tok> <tok cat="NN">cortisol</tok> <tok cat="NNS">concentrations</tok></cons></cons> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i9"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NNS">controls</tok></cons></cons><cons cat="ADVP" ref="i9" null="T"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
948370509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">plasma</tok> <tok cat="JJ">free</tok> <tok cat="NN">cortisol</tok> <tok cat="NNS">concentrations</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">similar</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">three</tok> <tok cat="NNS">groups</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
948370510@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">There</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">difference</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GR</tok></cons></cons> <cons cat="PP"><tok cat="IN">per</tok> <cons cat="NP"><tok cat="NN">cell</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">among</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">three</tok> <tok cat="NNS">groups</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">FMS</tok></cons><tok cat="COLON">:</tok> <cons cat="NP"><tok cat="CD">6498</tok> <tok cat="CC">+/-</tok> <tok cat="CD">252</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">LBP</tok></cons><tok cat="COLON">:</tok> <cons cat="NP"><tok cat="CD">6625</tok> <tok cat="CC">+/-</tok> <tok cat="CD">284</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">controls</tok></cons><tok cat="COLON">:</tok> <cons cat="NP"><tok cat="CD">6576</tok> <tok cat="CC">+/-</tok> <tok cat="CD">304</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">dissociation</tok> <tok cat="NN">constant</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Kd</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">FMS</tok> <cons cat="NP" ref="i24" null="RNR"></cons></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="QP"><tok cat="CD">14.5</tok> <tok cat="CC">+/-</tok> <tok cat="CD">0.9</tok></cons> <tok cat="NN">nmol/l</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">LBP</tok> <cons cat="NP" ref="i24" null="RNR"></cons></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="QP"><tok cat="CD">14.7</tok> <tok cat="CC">+/-</tok> <tok cat="CD">1.3</tok></cons> <tok cat="NN">nmol/l</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" id="i24"><tok cat="NNS">subjects</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><cons cat="ADJP"><tok cat="RB">significantly</tok> <tok cat="JJR">higher</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">controls</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="QP"><tok cat="CD">10.9</tok> <tok cat="CC">+/-</tok> <tok cat="CD">0.8</tok></cons> <tok cat="NN">nmol/l</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">p</tok></cons> <cons cat="ADJP"><tok cat="JJR">&lt;</tok> <cons cat="NP"><tok cat="CD">.05</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@80@@oe@16-12-2010
948370511@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">maximal</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">measured</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">maximal</tok> <tok cat="NN">thymidine</tok> <tok cat="NN">incorporation</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">absence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cortisol</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">FMS</tok> <tok cat="NN">group</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><cons cat="ADJP"><cons cat="QP"><tok cat="RB">approximately</tok> <tok cat="CD">1.5</tok> <tok cat="NNS">times</tok></cons> <tok cat="JJR">higher</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">p</tok></cons> <cons cat="ADJP"><tok cat="JJR">&lt;</tok> <cons cat="NP"><tok cat="CD">.05</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">control</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">LBP</tok> <tok cat="NN">group</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
948370512@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">ED50</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cortisol</tok> <tok cat="NN">concentration</tok></cons> <cons cat="VP"><tok cat="VBG">giving</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="CD">50</tok><tok cat="NN">%</tok></cons> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">thymidine</tok> <tok cat="NN">incorporation</tok></cons></cons></cons></cons></cons><tok cat="RRB">)</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">identical</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">all</tok> <tok cat="CD">three</tok> <tok cat="NNS">groups</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
948370513@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">FMS</tok> <tok cat="NNS">patients</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">mild</tok> <tok cat="NN">hypocortisolemia</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">cortisol</tok> <tok cat="NN">feedback</tok> <tok cat="NN">resistance</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">combination</tok></cons> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">probably</tok></cons> <tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">a</tok> <tok cat="VBN">reduced</tok> <tok cat="NN">CRH</tok> <cons cat="NP"><tok cat="NN">synthesis</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">release</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">hypothalamus</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
948370514@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GR</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">mineralocorticoid</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CRH</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">FMS</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">remains</tok> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">solved</tok><cons cat="NP" ref="i10" null="NONE"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
948804901@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Cytokine</tok></cons> <tok cat="NN">rescue</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">glucocorticoid</tok> <tok cat="JJ">induced</tok></cons> <tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IkappaBalpha</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@16-12-2010
948804902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBD">reported</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">dexamethasone</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">DEX</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">synthetic</tok> <tok cat="NN">glucocorticoid</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">causes</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="NN">Th</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">death</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">prevented</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">specific</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">namely</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Th1</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">IL-4</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Th2</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@48@@oe@16-12-2010
948804903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">differential</tok> <tok cat="NN">rescue</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">specific</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Th</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">correlates</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IkappaBalpha</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" id="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">DEX</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
948804904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons> <cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cellular</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IkappaBalpha</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><tok cat="NN">protein</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">evaluated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">DEX</tok> <tok cat="NN">treatment</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
948804905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Interestingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">DEX-mediated</tok> <cons cat="NP"><tok cat="NN">IkappaBalpha</tok></cons> <tok cat="NN">induction</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">completely</tok></cons> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok></cons><cons cat="PP" ref="i30" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP" id="i30"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Th1</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">reverse</tok> <tok cat="NN">profile</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">seen</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Th2</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
948804906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">cytokine</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IkappaBalpha</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">DEX</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">rescues</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">DEX-induced</tok> <tok cat="NN">apoptosis</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">rescue</tok> <tok cat="NN">cytokine</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">different</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Th1</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Th2</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
948804907@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">imply</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">need</tok> <cons cat="S"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">maintain</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">certain</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">in</tok> <tok cat="NN">order</tok> <cons cat="S"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">survive</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">up-</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">down-regulation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">NF</tok> <tok cat="NN">kappaB</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">modulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">IkappaBalpha</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NNS">cytokines</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">DEX</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">lead</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">survival</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">death</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@48@@oe@16-12-2010
949297701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">Genes</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">regulate</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-4</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@9@@oe@16-12-2010
949297702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Interleukin-4</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">immunomodulatory</tok> <tok cat="NN">cytokine</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">central</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">allergic</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">atopic</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="JJ">immune</tok> <tok cat="NNS">responses</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@16-12-2010
949297703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="JJ">Significant</tok> <tok cat="NN">progress</tok> <cons cat="PP" ref="i30" null="ICH"></cons></cons><cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">made</tok> <cons cat="NP" ref="i6" null="NONE"></cons></cons></cons></cons><cons cat="PP" id="i30"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">gaining</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">detailed</tok> <tok cat="NN">understanding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">interleukin-4</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
949297704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="DT">The</tok> <tok cat="JJ">recent</tok> <cons cat="NP"><tok cat="NN">identification</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">characterization</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">several</tok> <tok cat="JJ">key</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">helped</tok> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">elucidate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">helper</tok> <tok cat="NN">cell</tok> <tok cat="NN">cytokine</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
949749301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Endothelial</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MCP-1</tok></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">modulation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">heparin</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">consequences</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
949749302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Heparin</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">polyanionic</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glycosaminoglycan</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GAG</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NN">affinity</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">range</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons></cons> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interferon-gamma</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">chemokine</tok> <tok cat="NN">superfamily</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@31@@oe@16-12-2010
949749303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">GAG</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">possesses</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">immunomodulatory</tok> <tok cat="NN">activity</tok></cons> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">antagonize</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">capacity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">MHC</tok> <tok cat="NN">antigen</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VBP">up-regulate</tok> <cons cat="NP"><tok cat="JJ">intercellular</tok> <tok cat="NN">adhesion</tok> <tok cat="NN">molecule-1</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="VBN">cultured</tok> <tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
949749304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Previous</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">cell-surface</tok> <tok cat="NN">heparan</tok> <tok cat="NN">sulphate</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">optimal</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">free</tok> <tok cat="NN">heparin</tok></cons> <cons cat="ADVP"><tok cat="RB">competitively</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">sequestration</tok> <tok cat="NN">process</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
949749305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">performed</tok> <cons cat="NP" ref="i5" null="NONE"></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">increase</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">our</tok> <tok cat="NN">understanding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immunosuppressive</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">heparin</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">investigation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">potential</tok> <tok cat="NN">antagonism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">production</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">function</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">monocyte</tok> <tok cat="JJ">chemotactic</tok> <tok cat="NN">peptide-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MCP-1</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">chemokine</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NN">leucocyte</tok> <tok cat="NN">recruitment</tok></cons> <cons cat="PP"><tok cat="IN">across</tok> <cons cat="NP"><tok cat="JJ">vascular</tok> <tok cat="NN">endothelium</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
949749306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">mixture</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">heparin</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">up-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <tok cat="NN">signal</tok> <cons cat="NP"><tok cat="NN">transducer</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">activator</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">protein</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">STAT-1</tok></cons></cons> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">normally</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
949749307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">An</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">MCP-1</tok></cons> <tok cat="NN">production</tok></cons></cons> <cons cat="SBAR" ref="i30" null="ICH"></cons></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i6" null="NONE"></cons></cons></cons><cons cat="SBAR" id="i30"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" id="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="VP"><tok cat="VBN">caused</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">mixture</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">heparin-like</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons> <cons cat="ADJP"><tok cat="JJ">cytokine-binding</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="NNS">GAGs</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
949749308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">mixture</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">heparin-like</tok> <tok cat="NNS">GAGs</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">MCP-1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">rapid</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phosphatidylinositol</tok> <tok cat="NN">3-kinase</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" id="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">normally</tok></cons> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NNS">leucocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">this</tok> <cons cat="NP"><tok cat="NN">chemokine</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
949749309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="NN">Blockade</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">intracellular</tok> <tok cat="NN">signalling</tok> <tok cat="NN">event</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">reduction</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">normal</tok> <tok cat="JJ">transendothelial</tok> <tok cat="NN">migration</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="IN">towards</tok> <cons cat="NP"><tok cat="NN">MCP-1</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
949749310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Results</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">soluble</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">heparin-like</tok></cons></cons> <tok cat="NNS">GAGs</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">block</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">IFN-gamma-dependent</tok> <tok cat="NN">up-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">MCP-1</tok></cons> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="VBN">cultured</tok> <tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VB">antagonize</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">leucocyte-activating</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">migration-promoting</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">properties</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">pre-existing</tok> <cons cat="NP"><tok cat="NN">MCP-1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
949749311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">activities</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immunomodulatory</tok> <tok cat="NNS">properties</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">heparin</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
950773401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Xenogeneic</tok> <tok cat="JJ">human</tok> <tok cat="NN">serum</tok></cons> <cons cat="VP"><tok cat="VBZ">promotes</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">leukocyte</tok> <tok cat="NN">adhesion</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">porcine</tok> <tok cat="NN">endothelium</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><tok cat="NN">flow</tok> <tok cat="NNS">conditions</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">possibly</tok></cons> <tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@16-12-2010
950773402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="JJ">Endothelial</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">leukocyte</tok> <tok cat="NN">infiltration</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">consistent</tok> <tok cat="NN">feature</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">discordant</tok> <tok cat="NN">xenograft</tok> <tok cat="NN">rejection</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
950773403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">evaluated</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">xenogeneic</tok> <tok cat="NN">serum</tok><tok cat="COMMA">,</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">source</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">xenoreactive</tok> <tok cat="JJ">natural</tok> <tok cat="NNS">antibodies</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP" null="QSTN"></cons><tok cat="NN">complement</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">consequent</tok> <tok cat="NN">leukocyte</tok> <tok cat="NN">adhesion</tok></cons></cons> <cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><tok cat="NN">flow</tok> <tok cat="NNS">conditions</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
950773404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="JJ">Porcine</tok> <tok cat="JJ">aortic</tok> <tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">PAEC</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">incubated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">1</tok> <tok cat="NN">hr</tok> <tok cat="CD">30</tok> <tok cat="NN">min</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="CD">5</tok> <tok cat="NN">hr</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="CD">10</tok><tok cat="NN">%</tok></cons> <tok cat="JJ">homologous</tok> <tok cat="JJ">porcine</tok> <tok cat="NN">serum</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">control</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="CD">10</tok><tok cat="NN">%</tok></cons> <tok cat="JJ">xenogeneic</tok> <tok cat="JJ">human</tok> <tok cat="NN">serum</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">then</tok></cons> <cons cat="VP"><tok cat="VBN">perfused</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">total</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">leukocytes</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">parallel</tok> <tok cat="NN">plate</tok> <tok cat="NN">flow</tok> <tok cat="NN">chamber</tok></cons></cons> <cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">laminar</tok> <tok cat="NN">flow</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">1.5</tok> <tok cat="NNS">dynes/cm2</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@53@@oe@16-12-2010
950773405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="JJ">Adherent</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">counted</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">digital</tok> <tok cat="NN">image</tok> <tok cat="NN">analysis</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
950773406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Xenogeneic</tok> <tok cat="JJ">human</tok> <tok cat="NN">serum</tok></cons> <cons cat="ADVP"><cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">P</tok></cons> <cons cat="ADJP"><tok cat="JJR">&lt;</tok> <cons cat="NP"><tok cat="CD">0.01</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">adherent</tok> <tok cat="NNS">leukocytes</tok></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">porcine</tok> <tok cat="NN">serum</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
950773407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i18" role="SBJ"><tok cat="DT">A</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">similar</tok></cons> <cons cat="ADJP"><tok cat="JJ">adhesive</tok></cons></cons> <tok cat="NN">response</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">elicited</tok> <cons cat="NP" ref="i18" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="NN">TNF</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">100</tok> <tok cat="NN">U/ml</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RBS">most</tok> <tok cat="JJ">potent</tok></cons> <tok cat="NNS">inducers</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NN">cell</tok></cons> <tok cat="JJ">adhesive</tok> <tok cat="NNS">properties</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">here</tok></cons> <tok cat="VBN">used</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="JJ">positive</tok> <tok cat="NN">control</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
950773408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">In</tok> <tok cat="NN">order</tok> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">elucidate</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">possible</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="VP"><tok cat="VBG">underlying</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NN">cell</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">xenogeneic</tok> <tok cat="NN">serum</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">focussed</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">central</tok> <tok cat="NN">regulator</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">genes</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">adhesive</tok> <tok cat="NNS">molecules</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">chemoattractants</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
950773409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="NP"><tok cat="JJ">confocal</tok> <tok cat="NN">fluorescence</tok> <tok cat="NN">microscopy</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">positive</tok> <tok cat="NN">staining</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">p65</tok> <tok cat="NN">subunit</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">nuclei</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">PAEC</tok></cons> <cons cat="VP"><tok cat="VBN">exposed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="CD">1</tok> <tok cat="NN">hr</tok> <tok cat="CD">30</tok> <tok cat="NN">min</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">serum</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">indicated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">setting</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
950773410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">At</tok> <cons cat="NP"><tok cat="NN">variance</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">PAEC</tok></cons> <cons cat="VP"><tok cat="VBN">incubated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">homologous</tok> <tok cat="NN">serum</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">strictly</tok> <tok cat="JJ">localized</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cytoplasm</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
950773411@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">PAEC</tok></cons> <cons cat="VP"><tok cat="VBN">exposed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">xenogeneic</tok> <tok cat="NN">serum</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NNS">inhibitors</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">pyrrolidinedithiocarbamate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PDTC</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">25</tok> <tok cat="NN">microM</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tosyl-phechloromethylketone</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">TPCK</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">25</tok> <tok cat="NN">microM</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="ADVP"><cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">P</tok></cons> <cons cat="ADJP"><tok cat="JJR">&lt;</tok> <cons cat="NP"><tok cat="CD">0.01</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">reduced</tok> <cons cat="NP"><tok cat="NN">leukocyte</tok> <tok cat="NN">adhesion</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">respect</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">PAEC</tok></cons> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">serum</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@16-12-2010
950773412@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">Findings</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">xenogeneic</tok> <tok cat="NN">serum</tok></cons> <cons cat="VP"><tok cat="VBZ">promotes</tok> <cons cat="NP"><tok cat="JJ">leukocyte-endothelium</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">possibly</tok></cons> <tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">relevant</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">designing</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">future</tok></cons> <cons cat="ADJP"><tok cat="JJ">therapeutic</tok></cons></cons> <tok cat="NNS">strategies</tok></cons> <cons cat="VP"><tok cat="VBN">aimed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">prolonging</tok> <cons cat="NP"><tok cat="NN">xenograft</tok> <tok cat="NN">survival</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
951006401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">interleukin-2</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">alpha</tok> <tok cat="CC">and</tok> <tok cat="NN">beta</tok> <tok cat="NNS">isoforms</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@16-12-2010
951006402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">immunosuppressive</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><cons cat="ADVP"><tok cat="RB">largely</tok></cons> <tok cat="JJ">due</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="RB">immunologically</tok> <tok cat="JJ">relevant</tok> <tok cat="NNS">genes</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">interleukin-2</tok> <tok cat="LRB">(</tok><tok cat="NN">IL-2</tok><tok cat="RRB">)</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@25@@oe@16-12-2010
951006403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">effects</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="JJ">intracellular</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
951006404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NNS">humans</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">alternative</tok> <tok cat="NN">splicing</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GR</tok> <tok cat="NN">precursor</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">gives</tok> <cons cat="NP"><tok cat="NN">rise</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NN">receptor</tok> <tok cat="NNS">isoforms</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">termed</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" syn="COOD" role="PRD"><cons cat="NP"><tok cat="NN">GRalpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">GRbeta</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
951006405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">GRbeta</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">antagonize</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">GRalpha-mediated</tok> <tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">glucocorticoid-</tok><tok cat="JJ">responsive</tok> <tok cat="NN">element</tok> <tok cat="LRB">(</tok><tok cat="NN">GRE</tok><tok cat="RRB">)</tok><tok cat="JJ">-driven</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">COS-7</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
951006406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">designed</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">analyze</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">roles</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <cons cat="NP"><tok cat="NN">GR</tok> <tok cat="NNS">isoforms</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">glucocorticoid-mediated</tok> <tok cat="NN">transrepression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
951006407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">recently</tok> <tok cat="VBN">developed</tok></cons> <tok cat="NN">transfection</tok> <tok cat="NN">technique</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">human</tok> <tok cat="NNS">lymphocytes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="CD">548</tok> <tok cat="NN">bp</tok> <tok cat="NN">IL-2</tok> <tok cat="JJ">promoter-luciferase</tok> <tok cat="NN">reporter</tok> <tok cat="NN">construct</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">ester</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">calcium</tok> <tok cat="NN">ionophore</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">reversed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">dexamethasone</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">similar</tok> <tok cat="NN">extent</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NN">lymphoma</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">transfected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">GRalpha</tok></cons> <tok cat="NN">expression</tok> <tok cat="NN">vector</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@16-12-2010
951006408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Transfection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">GRbeta</tok></cons> <tok cat="NN">expression</tok> <tok cat="NN">vector</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">result</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons> <tok cat="NN">repression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
951006409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">GRbeta</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">antagonize</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">repressive</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GRalpha</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
951006410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Surprisingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">overexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GRbeta</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">cause</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">significant</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">GRalpha-induced</tok> <tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">GRE-dependent</tok> <tok cat="NN">luciferase</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">either</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
951006411@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transrepressive</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">transcription</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">exclusively</tok></cons> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">GRalpha</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
951006412@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">GRbeta</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <tok cat="CC">neither</tok> <cons cat="VP"><tok cat="VB">antagonize</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">GRalpha-mediated</tok> <tok cat="NN">transactivation</tok></cons> <tok cat="CC">nor</tok> <cons cat="NP"><cons cat="ADJP" null="QSTN"></cons><tok cat="NN">transrepression</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="NN">cell</tok> <tok cat="JJ">type-specific</tok></cons> <tok cat="NN">pattern</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">GRbeta-mediated</tok> <tok cat="NN">antiglucocorticoid</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
954848301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="JJ">Delta-opioid</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">enhance</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="NN">secretion</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">increasing</tok> <cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="NNS">complexes</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">NF-AT/AP-1-binding</tok> <tok cat="NN">promoter</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@22@@oe@16-12-2010
954848302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">Recent</tok></cons> <cons cat="ADJP"><tok cat="JJ">molecular</tok></cons></cons> <tok cat="NN">evidence</tok></cons> <cons cat="VP"><tok cat="VBZ">points</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">transient</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and/or</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">stage-specific</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">delta-</tok> <cons cat="ADJP" null="QSTN"></cons><cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">kappa-opioid</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">receptors</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">thymic</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">T</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@16-12-2010
954848303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Since</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="JJ">medical</tok> <tok cat="NNS">treatments</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">stress</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">commonly</tok></cons> <cons cat="VP"><tok cat="VBP">increase</tok> <cons cat="NP"><tok cat="JJ">opioid</tok> <tok cat="NNS">levels</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRP">it</tok></cons> <cons cat="S" ref="i30" null="EXP"></cons></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok></cons> <cons cat="S" id="i30"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">understand</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">opioids</tok></cons> <cons cat="VP"><tok cat="VBP">affect</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NNS">functions</tok></cons><cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
954848304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">created</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">studied</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="VBN">cloned</tok> <tok cat="JJ">delta-opioid</tok> <tok cat="NN">receptor</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">DOR1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
954848305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">DOR1</tok></cons> <tok cat="NN">ligation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">specific</tok> <cons cat="NP"><tok cat="NN">DOR1</tok></cons> <tok cat="NN">agonist</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">deltorphin</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">augmented</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="NN">secretion</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">synergizing</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">signals</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">TCR-CD3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CD28</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
954848306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="NN">Reporter</tok> <tok cat="NN">gene</tok> <tok cat="NNS">constructs</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i11" null="NONE"></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">map</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">deltorphin</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">AP-1-</tok> <cons cat="ADJP" null="QSTN"></cons><cons cat="NP" ref="i25" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">NF-AT/AP-1-binding</tok> <cons cat="NP" ref="i25" null="RNR"></cons></cons><cons cat="NP" id="i25"><tok cat="NNS">sites</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
954848307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">DOR1</tok></cons> <tok cat="NN">signaling</tok></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><tok cat="NN">[Ca2+]i</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">deltorphin</tok></cons> <cons cat="VP"><tok cat="VBD">enhanced</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">NF-AT/AP-1-binding</tok> <tok cat="NN">site</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">independent</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">calcineurin</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">distinct</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">elevated</tok> <tok cat="NN">[Ca2+]i</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
954848308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Deltorphin</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">accumulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NNS">complexes</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i13" role="SBJ"><tok cat="NN">DOR1</tok></cons> <cons cat="VP"><tok cat="VBZ">augments</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="NN">secretion</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i13" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">increasing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="NN">component</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-AT/AP-1</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
954848309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">advance</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">understanding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">opioid</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <cons cat="NP"><tok cat="NN">synthesis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">secretion</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">novel</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">receptor-mediated</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="NN">induction</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
954848501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">cyclic</tok> <tok cat="NN">AMP</tok> <tok cat="NN">response</tok> <tok cat="JJ">element-binding</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cyclic</tok> <tok cat="NN">AMP</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">NF-kappaB-mediated</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@16-12-2010
954848502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <tok cat="NN">family</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">regulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inducible</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">variety</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@16-12-2010
954848503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Recently</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">elevation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="JJ">cyclic</tok> <tok cat="NN">AMP</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">NF-kappaB-mediated</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">preventing</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NNS">complexes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
954848504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">inhibition</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
954848505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">hypothesized</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">A</tok></cons> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><tok cat="JJ">NF-kappaB-mediated</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">phosphorylating</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">proteins</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">cAMP</tok> <tok cat="NN">response</tok> <tok cat="JJ">element-binding</tok> <tok cat="NN">protein</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CREB</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">compete</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">limiting</tok> <tok cat="NNS">amounts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">coactivator</tok></cons> <cons cat="NP"><tok cat="NN">CBP</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
954848506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">amino-terminal</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">amino</tok> <tok cat="NNS">acids</tok> <tok cat="CD">1-450</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CBP</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="VP"><tok cat="VBZ">interacts</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">carboxyl-terminal</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">amino</tok> <tok cat="NNS">acids</tok> <tok cat="CD">286-551</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">p65</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RelA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="ADVP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
954848507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Functional</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">overexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CBP</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">rescued</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cAMP</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">NF-kappaB-mediated</tok> <tok cat="NN">transcription</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">chimeric</tok> <tok cat="NN">protein</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">GAL4-p65</tok><tok cat="LRB">(</tok><tok cat="CD">286-551</tok><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GAL4</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="VP"><tok cat="VBN">fused</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">carboxyl-terminal</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">amino</tok> <tok cat="NNS">acids</tok> <tok cat="CD">286-551</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@50@@oe@16-12-2010
954848508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">overexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CREB</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="JJ">GAL4-p65(286-551)-mediated</tok> <tok cat="NN">transcription</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
954848509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">A</tok></cons> <tok cat="NN">pathway</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">phosphorylating</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CREB</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i11"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">competes</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">limiting</tok> <tok cat="NNS">amounts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CBP</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
954849001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Differential</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DNA-binding</tok> <tok cat="NNS">activities</tok></cons></cons> <cons cat="PP"><tok cat="VBG">following</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">CD19</tok></cons> <tok cat="NN">cross-linking</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NN">lineage</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@16-12-2010
954849002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="JJ">cell-specific</tok> <tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <tok cat="NN">molecule</tok></cons></cons> <cons cat="NP"><tok cat="NN">CD19</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="NNS">stages</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">development</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">plasma</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">mediates</tok> <cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">cytoplasmic</tok> <tok cat="NN">effector</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@33@@oe@16-12-2010
954849003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="NOM" role="SBJ"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Cross-linking</tok> <cons cat="NP"><tok cat="NN">CD19</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">early</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NN">lineage</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">formation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">CD19/Vav/phosphatidylinositol-3</tok> <tok cat="NN">kinase</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CD19</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Vav</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Ras</tok></cons> <tok cat="NN">pathway</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
954849004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="ADVP"><tok cat="RB">further</tok></cons> <cons cat="VP"><tok cat="VB">explore</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">ramifications</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD19</tok></cons> <tok cat="NN">signaling</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">current</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Elk-1</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activator</tok> <tok cat="NN">protein-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AP-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappaB</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">occurred</tok> <cons cat="PP"><tok cat="VBG">following</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD19</tok></cons> <tok cat="NN">cross-linking</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
954849005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" null="NONE"></cons></cons></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="NP" syn="COOD" role="PRD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">BLIN-1</tok> <tok cat="JJ">pre-B</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">low</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <tok cat="NN">mu</tok> <tok cat="JJ">heavy</tok> <tok cat="NN">chain</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">surrogate</tok> <tok cat="NN">light</tok> <tok cat="NN">chain</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">1E8</tok> <tok cat="JJ">immature</tok> <tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <tok cat="NN">mu/kappa</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
954849006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Lysates</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD19</tok></cons> <tok cat="JJ">cross-linked</tok> <cons cat="NP"><tok cat="NN">1E8</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">robust</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">Elk-1</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Elk-1</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">occurred</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">lysates</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">CD19</tok> <tok cat="JJ">cross-linked</tok> <tok cat="NN">BLIN-1</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
954849007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">An</tok> <tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons> <cons cat="VP"><tok cat="VBG">employing</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">AP-1</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NN">consensus</tok> <tok cat="NNS">oligonucleotides</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i9" null="NONE"></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">AP-1 -binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBD">increased</tok><cons cat="PP" ref="i22" null="RNR"></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="JJ">constitutive</tok> <tok cat="JJ">NF-kappaB-binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">enhanced</tok><cons cat="NP" ref="i10" null="NONE"></cons><tok cat="COMMA">,</tok> <cons cat="PP" id="i22"><tok cat="VBG">following</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">2</tok> <tok cat="NN">h</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">CD19</tok></cons> <tok cat="NN">cross-linking</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">1E8</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
954849008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Supershift</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">JunD</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Fos</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="VP"><tok cat="VBD">mediated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">anti-CD19</tok> <tok cat="JJ">induced</tok> <tok cat="JJ">AP-1-binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">1E8</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
954849009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">CD19</tok></cons> <tok cat="NN">cross-linking</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">BLIN-1</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="RB">no</tok> <tok cat="JJ">apparent</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">AP-1-binding</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
954849010@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">CD19-mediated</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="JJ">different</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factors</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">juxtaposed</tok> <tok cat="NNS">stages</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">development</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i14"><tok cat="IN">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i14" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">culminate</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">activation</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">suppression</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">distinct</tok> <tok cat="NNS">sets</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
955038501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Pharmacological</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">antigen</tok> <tok cat="NN">responsiveness</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="RB">genetically</tok> <tok cat="VBN">modified</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@11@@oe@16-12-2010
955038502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <cons cat="NP"><tok cat="JJ">chimeric</tok> <tok cat="NN">TCR</tok> <tok cat="NN">gene</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">comprising</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">anti-hapten</tok> <tok cat="JJ">single-chain</tok> <tok cat="NN">Ab</tok> <tok cat="JJ">variable</tok> <tok cat="NN">fragment</tok></cons></cons> <cons cat="VP"><tok cat="VBN">fused</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">transmembrane</tok> <tok cat="CC">and</tok> <tok cat="JJ">cytoplasmic</tok> <tok cat="NNS">regions</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">TCR</tok> <tok cat="NN">zeta-chain</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i5" null="NONE"></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">tetracycline-regulatable</tok> <tok cat="NN">system</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i6" null="NONE"></cons></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@44@@oe@16-12-2010
955038503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">stably</tok></cons> <cons cat="VP"><tok cat="VBN">transfected</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">single</tok> <tok cat="NN">vector</tok></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">tetracycline</tok> <tok cat="JJ">trans-activator</tok> <tok cat="NN">protein</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">controlled</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">constitutive</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">chimeric</tok> <tok cat="NN">TCR</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">trans-activator</tok> <tok cat="JJ">protein-responsive</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
955038504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">absence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">tetracyclines</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><tok cat="DT">the</tok> <tok cat="VBN">transfected</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">express</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">chimeric</tok> <tok cat="NN">receptor</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">surface</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="PRPP">its</tok> <cons cat="NP"><tok cat="JJ">cognate</tok> <tok cat="NN">Ag</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">secretion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
955038505@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i3"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">exposed</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">increasing</tok> <tok cat="NNS">concentrations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">tetracyclines</tok></cons></cons></cons></cons><cons cat="ADVP" ref="i3" null="T"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="NN">surface</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">chimeric</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">suppressed</tok> <cons cat="NP" ref="i14" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">dose-dependent</tok> <tok cat="NN">manner</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">suppression</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">sufficient</tok></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">result</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">complete</tok> <tok cat="NN">loss</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">responsiveness</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="VBN">targeted</tok> <tok cat="NN">Ag</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
955038506@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i15" role="SBJ"><cons cat="NP"><tok cat="JJ">Prolonged</tok> <tok cat="NN">suppression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">trans-gene</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="QP"><tok cat="RB">up</tok> <tok cat="TO">to</tok> <tok cat="CD">7</tok></cons> <tok cat="NNS">days</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i15" null="NONE"></cons></cons><cons cat="SBAR" func="ADV" adv="TMP"><tok cat="IN">after</tok> <cons cat="S"><cons cat="NP" id="i16" role="SBJ"><tok cat="NN">doxycycline</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">removed</tok> <cons cat="NP" ref="i16" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cultures</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">eventual</tok> <tok cat="NN">recovery</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">surface</tok> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">complete</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">absolute</tok> <tok cat="NN">time</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">recovery</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">directly</tok> <tok cat="JJ">proportional</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">initial</tok> <tok cat="NN">concentration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">drug</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@16-12-2010
955038507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Pharmacologic</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">trans-gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">gene-modified</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="MD">will</tok> <cons cat="CONJP"><tok cat="RB">not</tok> <tok cat="RB">only</tok></cons> <cons cat="VP"><tok cat="VB">facilitate</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">new</tok> <tok cat="NNS">approaches</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">study</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">aspects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">biology</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="MD">will</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VB">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">basis</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">new</tok> <tok cat="NN">gene</tok> <tok cat="NN">therapy</tok> <tok cat="NNS">strategies</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
955042601@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons></cons> <cons cat="VP"><tok cat="VBP">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="NN">IL-1</tok> <tok cat="NN">receptor</tok> <cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">I</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">II</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">IL-4</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-13</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@16-12-2010
955042602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">Th2-type</tok> <tok cat="NNS">cytokines</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-4</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-13</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">distinct</tok> <tok cat="NN">subset</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">genes</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@16-12-2010
955042603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok></cons> <cons cat="VP"><tok cat="VBP">include</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">Fc</tok> <tok cat="NN">epsilonRII</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CD23</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">15-lipoxygenase</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="NN">receptor</tok> <tok cat="NN">antagonist</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-1ra</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">I</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">II</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-1R</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
955042604@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="NN">IFN-gamma</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CD23</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">15-lipoxygenase</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">I</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">II</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NN">IL-1R</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">defined</tok><cons cat="NP" ref="i8" null="NONE"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
955042605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">basal</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">IL-4/IL-13-induced</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><cons cat="NP"><tok cat="NN">IL-1R</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
955042606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">IL-4</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-13</tok></cons></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">dose-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">time-dependent</tok></cons></cons> <tok cat="NNS">increases</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-1RI</tok> <cons cat="NP" ref="i24" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-1RII</tok> <cons cat="NP" ref="i24" null="RNR"></cons></cons><cons cat="NP" id="i24"><tok cat="NN">mRNA</tok> <tok cat="NNS">levels</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
955042607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IFN-gamma</tok></cons> <cons cat="VP"><tok cat="VBD">decreased</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">basal</tok> <tok cat="NN">expression</tok></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">IL-4</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-13</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
955042608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">IL-1RI</tok></cons> <cons cat="NP" ref="i26" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-1RII</tok> <cons cat="NP" ref="i26" null="RNR"></cons></cons><cons cat="NP" id="i26"><tok cat="NN">mRNA</tok> <tok cat="NNS">levels</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">IFN-gamma</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">transcriptionally</tok></cons> <cons cat="VP"><tok cat="VBN">mediated</tok><cons cat="NP" ref="i14" null="NONE"></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">correlated</tok> <cons cat="ADVP"><tok cat="RB">directly</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">decreased</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">soluble</tok> <cons cat="NP"><tok cat="NN">IL-1RII</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
955042609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">suppress</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-1RI</tok> <cons cat="NP" ref="i27" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-1RII</tok></cons> <cons cat="NP" ref="i27" null="RNR"></cons></cons><cons cat="NP" id="i27"><tok cat="NN">mRNA</tok> <tok cat="NNS">levels</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unique</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">because</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-10</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">IL-4/IL-13-stimulated</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
955042610@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-1R</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">due</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">down-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">surface</tok> <tok cat="NN">IL-4R</tok></cons></cons></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">because</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">pretreatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cytokines</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">decrease</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-4</tok></cons> <tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons></cons> <cons cat="PP"><tok cat="IN">per</tok> <cons cat="NP"><tok cat="NN">cell</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
955042611@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i15" role="SBJ"><cons cat="NP"><tok cat="NN">suppression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-1R</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="VBN">decreased</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">translocation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">IL-4/IL-13-inducible</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Stat6</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potential</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">mediate</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">suppressive</tok> <tok cat="NNS">effects</tok></cons><cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
955042612@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">certain</tok> <tok cat="NNS">cytokines</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-10</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">antagonize</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-4</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-13</tok></cons></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-1RI</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-1RII</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
957051201@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok> <tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok> <tok cat="NNS">quasispecies</tok></cons> <cons cat="VP"><tok cat="VBP">differ</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">basal</tok> <tok cat="NN">transcription</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons> <tok cat="NN">recruitment</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">human</tok> <cons cat="NP"><tok cat="JJ">glial</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@16-12-2010
957051202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">generation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">genomic</tok> <tok cat="NN">diversity</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">course</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">infection</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">potential</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="NNS">aspects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">replication</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">proviral</tok> <tok cat="NN">genome</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@28@@oe@16-12-2010
957051203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i14" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">gain</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJR">better</tok> <tok cat="NN">understanding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">impact</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LTR</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">sequence</tok> <tok cat="NN">diversity</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">LTR-directed</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">central</tok> <tok cat="JJ">nervous</tok> <tok cat="NN">system</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CNS</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">immune</tok> <tok cat="NN">system</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i14" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">amplified</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">cloned</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP"><cons cat="NP" id="i20"><tok cat="NNS">LTRs</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">proviral</tok> <tok cat="NN">DNA</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">HIV-1-infected</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@48@@oe@16-12-2010
957051204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Sequence</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">nineteen</tok> <cons cat="NP"><tok cat="NNS">LTRs</tok></cons></cons> <cons cat="VP"><tok cat="VBN">cloned</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">2</tok> <tok cat="JJ">adult</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">3</tok> <tok cat="JJ">pediatric</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">average</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">33</tok> <tok cat="NN">nucleotide</tok> <tok cat="NNS">changes</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">respect</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">sequence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">LAI</tok> <tok cat="NN">LTR</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">455-bp</tok> <tok cat="NN">U3</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
957051205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Transient</tok> <tok cat="NN">expression</tok> <tok cat="NNS">analyses</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">neuroglial</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">lymphocytic</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NN">origin</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">some</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NNS">LTRs</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">activities</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">varied</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">LAI</tok> <tok cat="NN">LTR</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">U-373</tok> <tok cat="NN">MG</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">astrocytoma</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">CD4-</tok><tok cat="JJ">positive</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@50@@oe@16-12-2010
957051206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">While</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">LTRs</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJS">highest</tok> <tok cat="NNS">activities</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">U-373</tok> <tok cat="NN">MG</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">yielded</tok> <cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">activities</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NNS">LTRs</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">generally</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="RBR">more</tok> <tok cat="JJ">active</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i17"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">LAI</tok> <tok cat="NN">LTR</tok></cons></cons></cons><cons cat="ADVP" ref="i17" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
957051207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Differences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">LTR</tok> <tok cat="NN">sequence</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">differences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">recruitment</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cis-acting</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">U3</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">LTR</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
957051208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">particular</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="ADJP"><tok cat="RB">naturally</tok> <tok cat="VBG">occurring</tok></cons> <tok cat="NN">sequence</tok> <tok cat="NN">variation</tok></cons> <cons cat="VP"><tok cat="JJ">impacted</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="VBG">activating</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor/cAMP</tok> <tok cat="NN">response</tok> <tok cat="NN">element</tok> <tok cat="NN">binding</tok> <tok cat="LRB">(</tok><tok cat="NN">ATF/CREB</tok><tok cat="RRB">)</tok> <tok cat="NN">binding</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">LEF-1</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">distal</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i7" null="T"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">previous</tok> <tok cat="NNS">studies</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">LTR</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@48@@oe@16-12-2010
957051209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">LTR</tok> <tok cat="NN">sequence</tok></cons> <tok cat="NNS">changes</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">basal</tok> <cons cat="NP"><tok cat="NN">LTR</tok></cons> <tok cat="NN">function</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <tok cat="NN">recruitment</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">may</tok><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">turn</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VB">alter</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">course</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">replication</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CNS</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">immune</tok> <tok cat="NN">system</tok> <tok cat="NN">origin</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
957299001@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Stat-3</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">ligation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">major</tok> <tok cat="NN">histocompatibility</tok> <tok cat="NN">complex</tok> <tok cat="NN">class</tok> <tok cat="NN">I</tok> <tok cat="NNS">molecules</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@25@@oe@16-12-2010
957299002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Janus</tok> <tok cat="NN">tyrosine</tok> <tok cat="NNS">kinases</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Jak</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Signal</tok> <tok cat="NNS">transducers</tok> <tok cat="CC">and</tok> <tok cat="NNS">activators</tok> <tok cat="IN">of</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">Stat</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">ligation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">major</tok> <tok cat="NN">histocompatibility</tok> <tok cat="NN">complex</tok> <tok cat="NN">class</tok> <tok cat="CD">I</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MHC-I</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">explored</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@36@@oe@16-12-2010
957299003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Cross-linking</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MHC-I</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">mediated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Tyk2</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Jak1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Jak2</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Jak3</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
957299004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="NP"><tok cat="NN">Stat-3</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="NN">tyrosine</tok> <tok cat="VBN">phosphorylated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytoplasm</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">subsequently</tok></cons> <tok cat="VBN">translocated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">nucleus</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
957299005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Data</tok></cons> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">suggested</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">Stat-3</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">associates</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">serum-inducible</tok> <tok cat="NN">element</tok> <tok cat="LRB">(</tok><tok cat="NN">hSIE</tok><tok cat="RRB">)</tok> <tok cat="NN">DNA-probe</tok></cons></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">interferon-gamma</tok> <tok cat="JJ">activated</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GAS</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">c-fos</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">common</tok> <tok cat="NN">DNA</tok> <tok cat="NN">sequence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">Stat</tok> <tok cat="NN">protein</tok></cons> <tok cat="NN">binding</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@16-12-2010
957299006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">An</tok> <tok cat="NN">association</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">hSIE</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Stat-3</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">MHC-I</tok></cons> <tok cat="NN">ligation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">directly</tok></cons> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">precipitating</tok> <cons cat="NP"><tok cat="NN">Stat-3</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">biotinylated</tok> <cons cat="NP"><tok cat="NN">hSIE</tok> <tok cat="NN">probe</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">avidin-coupled</tok> <tok cat="NN">agarose</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
957299007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">investigate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">activated</tok> <cons cat="NP"><tok cat="NN">Stat-3</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">transiently</tok></cons> <cons cat="VP"><tok cat="VBN">transfected</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">Stat-3</tok> <tok cat="NN">isoform</tok></cons></cons> <cons cat="VP"><tok cat="VBG">lacking</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="VBG">transactivating</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
957299008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <cons cat="NP"><tok cat="JJ">dominant-negative</tok> <tok cat="VBG">acting</tok> <tok cat="NN">Stat-3</tok> <tok cat="NN">isoform</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">ligation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MHC-I</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
957299009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">conclusion</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Jak/Stat</tok></cons> <tok cat="NN">signal</tok> <tok cat="NN">pathway</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">MHC-I-induced</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
961615901@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappaB</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">attenuates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">apoptosis</tok> <tok cat="NN">resistance</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">lymphoid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@16-12-2010
961615902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Death-inducing</tok> <tok cat="NNS">ligands</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">DILs</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNFalpha</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cytotoxic</tok> <tok cat="NN">drug</tok></cons> <cons cat="NP"><tok cat="NN">doxorubicin</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappaB</tok></cons> <tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NFkappaB</tok></cons><tok cat="RRB">)</tok><tok cat="JJ">-dependent</tok> <tok cat="NN">program</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">rescue</tok> <cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok> <tok cat="NN">induction</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@41@@oe@16-12-2010
961615903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">TRAIL</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">TNF-related</tok> <tok cat="NN">apoptosis-inducing</tok> <tok cat="NN">ligand</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">recently</tok> <tok cat="VBN">identified</tok></cons> <cons cat="NP"><tok cat="NN">DIL</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NFkappaB</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">lymphoid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">kinetic</tok></cons> <cons cat="ADJP"><tok cat="JJ">similar</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">TNFalpha</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
961615904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">NFkappaB</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">independent</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">FADD</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNS">caspases</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok> <tok cat="NN">induction</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
961615905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">study</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">influence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NFkappaB</tok></cons> <tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">TRAIL</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CD95</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">TNFalpha</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">doxorubicin</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">NFkappaB</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i6" null="NONE"></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">proteasome</tok></cons> <tok cat="NN">inhibitor</tok></cons> <cons cat="NP"><tok cat="NN">N-acetyl-L-leucinyl-L-leucinyl-L-norleucinal</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">overexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">mutant</tok> <tok cat="NN">IkappaBalpha</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
961615906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">Sensitivity</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">markedly</tok></cons> <cons cat="VP"><tok cat="VBN">increased</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">treatments</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">apoptosis</tok> <tok cat="JJ">sensitive</tok></cons> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
961615907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP" syn="COOD"><tok cat="CC">both</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NNS">lines</tok> <cons cat="SBAR" ref="i30" null="RNR"></cons></cons></cons><tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">leukemia</tok> <tok cat="NNS">cells</tok> <cons cat="SBAR" ref="i30" null="RNR"></cons></cons></cons><cons cat="SBAR" id="i30"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">resistant</tok> <cons cat="PP"><tok cat="IN">towards</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">DILs</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">doxorubicin</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">antagonization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NFkappaB</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">partially</tok></cons> <cons cat="VP"><tok cat="VBD">restored</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok> <tok cat="NN">sensitivity</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
961615908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NFkappaB</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">approach</tok></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i9" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">increase</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok> <tok cat="NN">sensitivity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">anticancer</tok> <tok cat="NN">treatment</tok></cons></cons><cons cat="ADVP" ref="i9" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
961616301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" syn="COOD" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">MLL</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CALM</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">fused</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">AF10</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">morphologically</tok> <tok cat="JJ">distinct</tok></cons> <tok cat="NNS">subsets</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="NN">leukemia</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">translocation</tok> <tok cat="NN">t(10;11)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">both</tok> <tok cat="NNS">rearrangements</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">poor</tok> <tok cat="NN">prognosis</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@16-12-2010
961616302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">translocation</tok> <tok cat="NN">t(10;11)(p13;q14)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">lymphoblastic</tok> <tok cat="NN">leukemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ALL</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">leukemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AML</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@16-12-2010
961616303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">A</tok> <tok cat="JJ">recent</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">MLL/AF10</tok> <tok cat="NN">fusion</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="NNS">cases</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AML</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">t(10;11)</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NNS">breakpoints</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="CD">11</tok></cons></cons> <cons cat="VP"><tok cat="VBG">ranging</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">q13</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">q23</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
961616304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP"><tok cat="VBN">cloned</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">CALM</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Clathrin</tok> <tok cat="NN">Assembly</tok> <tok cat="JJ">Lymphoid</tok> <tok cat="JJ">Myeloid</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">gene</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">fusion</tok> <tok cat="NN">partner</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AF10</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">11q14</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">U937</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
961616305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="ADVP"><tok cat="RB">further</tok></cons> <cons cat="VP"><tok cat="VB">define</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="NNS">leukemias</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i9" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="CD">10</tok> <tok cat="NNS">cases</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">9</tok> <tok cat="NN">AML</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">1</tok> <tok cat="NN">ALL</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">cytogenetically</tok> <tok cat="JJ">proven</tok></cons> <cons cat="NP"><tok cat="NN">t(10;11)(p12-14;q13-21)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP" syn="COOD"><tok cat="VBD">well-characterized</tok> <cons cat="NP"><tok cat="NN">morphology</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">immunophenotype</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">clinical</tok> <tok cat="NN">course</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok><cons cat="NP" ref="i9" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
961616306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="NN">Interphase</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">fluorescence</tok> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">situ</tok></cons> <tok cat="NN">hybridization</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">FISH</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">performed</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="CD">2</tok> <cons cat="NP"><tok cat="NNS">YACs</tok></cons></cons> <cons cat="VP"><tok cat="VBG">flanking</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CALM</tok> <tok cat="NN">region</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">YAC</tok> <tok cat="NN">contig</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">MLL</tok> <tok cat="NN">region</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">YAC</tok></cons></cons> <cons cat="VP"><tok cat="VBG">spanning</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">AF10</tok></cons> <tok cat="NN">breakpoint</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
961616307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="NN">Rearrangement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok> <tok cat="CD">one</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="NNS">cases</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">balanced</tok> <cons cat="NP"><tok cat="NN">t(10;11)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
961616308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">4</tok> <tok cat="NNS">cases</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="CD">3</tok> <tok cat="NN">AML</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">immature</tok> <tok cat="NN">morphology</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">1</tok> <tok cat="NN">AML-M0</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">2</tok> <tok cat="NN">AML-M1</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">1</tok> <tok cat="NN">ALL</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">signals</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CALM</tok> <tok cat="NNS">YACS</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">separated</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">interphase</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">translocation</tok> <tok cat="NN">breakpoint</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CALM</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
961616309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i13" role="SBJ"><tok cat="NN">MLL</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">rearranged</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">3</tok> <tok cat="NN">AML</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">myelomonocytic</tok> <tok cat="NN">differentiation</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">2</tok> <tok cat="NN">AML-M2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">1</tok> <tok cat="NN">AML-M5</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><tok cat="CD">1</tok> <tok cat="JJ">secondary</tok> <tok cat="NN">AML</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
961616310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">all</tok> <tok cat="CD">3</tok> <tok cat="NNS">cases</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i14" role="SBJ"><tok cat="DT">a</tok> <tok cat="JJ">characteristic</tok> <tok cat="NN">immunophenotype</tok> <cons cat="PRN" ref="i30" null="ICH"></cons></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i14" null="NONE"></cons></cons></cons><cons cat="PRN" id="i30"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">CD4+</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CD13-</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">CD33+</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">CD65s+</tok></cons></cons><tok cat="RRB">)</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
961616311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i15" role="SBJ"><tok cat="NN">AF-10</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">5</tok> <tok cat="IN">of</tok> <tok cat="CD">6</tok></cons> <tok cat="JJ">evaluable</tok> <tok cat="NNS">cases</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">1</tok> <tok cat="NN">case</tok></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">detectable</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CALM</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">MLL</tok> <tok cat="NN">rearrangement</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
961616312@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="CD">2</tok> <tok cat="NN">complex</tok> <tok cat="NNS">translocations</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i16" role="SBJ"><cons cat="NP"><tok cat="NN">none</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">three</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">rearranged</tok><cons cat="NP" ref="i16" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
961616313@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">All</tok> <tok cat="NNS">cases</tok></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">remarkably</tok> <tok cat="JJ">poor</tok></cons> <tok cat="NN">prognosis</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">mean</tok> <tok cat="NN">survival</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="QP"><tok cat="CD">9.6</tok> <tok cat="CC">+/-</tok> <tok cat="CD">6.6</tok></cons> <tok cat="NNS">months</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
961616314@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="PP"><tok cat="IN">For</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">7</tok> <tok cat="NN">AML</tok> <tok cat="NNS">cases</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" id="i17" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">uniformly</tok></cons> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" ref="i17" null="NONE"></cons><cons cat="PP"><tok cat="VBG">according</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">AMLCG86/92</tok> <tok cat="NNS">protocols</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">disease-free</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">overall</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">survival</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="RB">significantly</tok> <tok cat="JJ">worse</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">overall</tok> <tok cat="NN">study</tok> <tok cat="NN">group</tok></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">P</tok></cons> <cons cat="ADJP"><tok cat="JJ">=</tok> <cons cat="NP"><tok cat="CD">.03</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">P</tok></cons> <cons cat="ADJP"><tok cat="JJ">=</tok> <cons cat="NP"><tok cat="CD">.01</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons><tok cat="RRB">)</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
961616315@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i18" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">t(10;11)(p13;q14)</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">indicates</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CALM</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">MLL</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">rearrangements</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">morphologically</tok> <tok cat="JJ">distinct</tok></cons> <tok cat="NNS">subsets</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="NN">leukemia</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i18" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">poor</tok> <tok cat="NN">prognosis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
961875801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">HMG</tok> <tok cat="NN">box</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lymphocyte</tok></cons> <tok cat="NN">differentiation</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@9@@oe@16-12-2010
961875802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">identification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">mammalian</tok> <tok cat="JJ">sex-determining</tok> <tok cat="NN">gene</tok> <tok cat="NN">Sry</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">led</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">discovery</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">large</tok> <tok cat="NN">family</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><cons cat="ADJP"><tok cat="JJ">related</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><tok cat="LQT">'</tok><cons cat="NP"><tok cat="NN">HMG</tok> <tok cat="NN">box</tok></cons><tok cat="RQT">'</tok><tok cat="RRB">)</tok></cons></cons> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">control</tok> <cons cat="NP"><tok cat="JJ">developmental</tok> <tok cat="NNS">events</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">yeast</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="FW">C.</tok> <tok cat="FW">elegans</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="FW">Drosophila</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">vertebrates</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@41@@oe@16-12-2010
961875803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lymphocyte</tok></cons> <tok cat="NN">differentiation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="JJ">several</tok> <cons cat="NP"><tok cat="NN">HMG</tok> <tok cat="NN">box</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="VP"><tok cat="VBP">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">decisive</tok> <tok cat="NN">role</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
961875804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Sox-4</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">very</tok> <tok cat="JJ">early</tok></cons> <tok cat="NN">B-cell</tok> <tok cat="NN">differentiation</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">TCF-1/LEF-1</tok></cons> <cons cat="VP"><tok cat="VBP">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">crucial</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">thymocyte</tok> <tok cat="NN">development</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
961875805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">TCF/LEF</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">constitute</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">downstream</tok> <tok cat="NN">component</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Wingless/Wnt</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
961875806@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NNS">flies</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">pathway</tok></cons> <cons cat="VP"><tok cat="VBZ">controls</tok> <cons cat="NP"><tok cat="NN">segment</tok> <tok cat="NN">polarity</tok></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="FW">Xenopus</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">controls</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">definition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">body</tok> <tok cat="NN">axis</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
961875807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Deregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathway</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">several</tok> <tok cat="JJ">human</tok> <tok cat="NNS">tumors</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
961875808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">These</tok> <tok cat="VBZ">insights</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NNS">events</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" id="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">TCF/LEF</tok></cons> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">organisms</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="ADVP"><tok cat="RB">eventually</tok></cons> <cons cat="VP"><tok cat="VB">lead</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">understanding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NN">HMG</tok> <tok cat="NN">box</tok> <tok cat="NNS">proteins</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">lymphoid</tok> <tok cat="NN">development</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
961875901@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Loss-</tok> <cons cat="ADJP" null="QSTN"></cons><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">gain-of-function</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">mutations</tok></cons></cons> <cons cat="VP"><tok cat="VBP">reveal</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">BSAP</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Pax-5</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">start</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">end</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@16-12-2010
961875902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Pax-5</tok></cons> <cons cat="VP"><tok cat="VBZ">codes</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="NP"><tok cat="NN">BSAP</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" id="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">throughout</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">development</tok></cons></cons> <cons cat="PP"><tok cat="NN">except</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="RB">terminally</tok> <tok cat="VBN">differentiated</tok> <tok cat="NN">plasma</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@16-12-2010
961875903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Gene</tok> <tok cat="NN">targeting</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mouse</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">differential</tok> <tok cat="NN">dependency</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">fetal</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">adult</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">B-lymphopoiesis</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">this</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
961875904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">BSAP</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">B-lineage</tok> <tok cat="NN">commitment</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">fetal</tok> <tok cat="NN">liver</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">progression</tok></cons> <cons cat="PP"><tok cat="IN">beyond</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">early</tok> <tok cat="NN">pro-B</tok> <tok cat="NN">cell</tok> <tok cat="NN">stage</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="NN">bone</tok> <tok cat="NN">marrow</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
961875905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">characterization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">Pax-5-deficient</tok> <tok cat="NN">pro-B</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">BSAP</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD19</tok></cons><cons cat="NP" null="QSTN"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">mb-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Ig</tok> <tok cat="NN">alpha</tok></cons><tok cat="RRB">)</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">N-myc</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">developmental</tok> <tok cat="NN">pathway</tok></cons> <cons cat="VP"><tok cat="VBG">controlling</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">VH-to-DHJH</tok> <tok cat="NN">recombination</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">immunoglobulin</tok> <tok cat="JJ">heavy-chain</tok> <tok cat="LRB">(</tok><tok cat="NN">IgH</tok><tok cat="RRB">)</tok> <tok cat="NN">locus</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@16-12-2010
961875906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">PAX-5</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">participate</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">together</tok></cons> <tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IgH</tok> <tok cat="NN">locus</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">chromosomal</tok> <tok cat="NN">translocation</tok> <cons cat="NP"><tok cat="NN">t(9;14)(p13;q32)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
961875907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">translocation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">characteristic</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">small</tok> <tok cat="NN">subset</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">non-Hodgkin</tok> <tok cat="NNS">lymphomas</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBG">exhibiting</tok> <cons cat="NP"><tok cat="JJ">plasmacytoid</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
961875908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">translocated</tok> <tok cat="NN">PAX-5</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">deregulated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">insertion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IgH</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">elements</tok></cons></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <cons cat="NP"><tok cat="JJ">5'</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" id="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">tumorigenesis</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">interfering</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">shut-down</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PAX-5</tok></cons> <tok cat="NN">transcription</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">completion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">plasma</tok> <tok cat="NN">cell</tok></cons> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
961876001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">E-proteins</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">B-</tok> <cons cat="NP" null="QSTN"></cons><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T-lymphocyte</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">development</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@10@@oe@16-12-2010
961876002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">Department</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NN">stem</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">controlled</tok><cons cat="NP" ref="i5" null="NONE"></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">part</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@16-12-2010
961876003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">particular</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok> <tok cat="NN">class</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">helix-loop-helix</tok> <tok cat="NNS">proteins</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">termed</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NNS">E-proteins</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">development</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
961876004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Recent</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">gene-targeted</tok> <tok cat="NNS">mice</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">allowed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">direct</tok> <tok cat="NN">assessment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">functional</tok> <tok cat="NNS">roles</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NN">E-protein</tok> <tok cat="NN">family</tok> <tok cat="NNS">members</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">hematopoiesis</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
961876005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">review</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">describe</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">defects</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">B-</tok> <cons cat="NP" null="QSTN"></cons><cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T- lymphocyte</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">development</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">mice</tok></cons> <cons cat="VP"><tok cat="VBG">carrying</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">targeted</tok> <tok cat="NNS">mutations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">E-protein</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">discuss</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">our</tok> <tok cat="JJ">current</tok> <tok cat="NN">understanding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">lymphoid</tok> <tok cat="NN">development</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
961991801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">IL-2-induced</tok> <tok cat="NN">growth</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">CD8+</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="JJ">prolymphocytic</tok> <tok cat="NN">leukemia</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <tok cat="NN">induction</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
961991802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-2</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappaB</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">receptor</tok> <tok cat="LRB">(</tok><tok cat="NN">IL-2R</tok><tok cat="RRB">)</tok> <tok cat="NN">alpha</tok> <tok cat="NN">chain</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">proliferation</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@41@@oe@16-12-2010
961991803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2R</tok></cons> <tok cat="NN">signaling</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">growth</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">CD8+</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="JJ">prolymphocytic</tok> <tok cat="NN">leukemia</tok> <tok cat="LRB">(</tok><tok cat="NN">T-PLL</tok><tok cat="RRB">)</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">investigated</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
961991804@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Flow</tok> <tok cat="NN">cytometry</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">leukemia</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">patient</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">CD8+</tok> <tok cat="NN">T-PLL</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2Ralpha</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><tok cat="NN">beta</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">chains</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">proliferative</tok> <tok cat="NN">response</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">IL-2Ralpha</tok> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">culture</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">exogeneous</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
961991805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="NN">Northern</tok> <tok cat="NN">blot</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">failed</tok> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">detect</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-2</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">act</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">paracrine</tok> <tok cat="NN">manner</tok></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
961991806@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Electrophoretic</tok> <tok cat="NN">mobility-shift</tok> <tok cat="NNS">assays</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">recombinant</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">leukemia</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Northern</tok> <tok cat="NN">blot</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-2</tok></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">abundance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">mRNAs</tok></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <tok cat="NNS">components</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Rel</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">KBF1</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
961991807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="NN">binding</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-2</tok></cons> <cons cat="ADVP"><tok cat="RB">markedly</tok></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">low</tok> <tok cat="NN">affinity</tok> <cons cat="NP"><tok cat="NNS">IL-2Rs</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">leukemia</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">high-affinity</tok> <tok cat="NNS">IL-2Rs</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
961991808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="NN">IL-2</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inducing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">CD8+</tok> <tok cat="NN">T-PLL</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">effect</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok><cons cat="NP" ref="i10" null="NONE"></cons><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">part</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">pretranslational</tok> <tok cat="NN">level</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
962577001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">human</tok> <tok cat="NN">toll</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok><tok cat="COLON">:</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">divergence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappaB</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">JNK/SAPK</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="ADVP"><tok cat="RB">upstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="JJ">receptor-associated</tok> <tok cat="NN">factor</tok> <tok cat="CD">6</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TRAF6</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@26@@oe@16-12-2010
962577002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">human</tok> <tok cat="NN">homologue</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="FW">Drosophila</tok> <tok cat="NN">Toll</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">hToll</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">recently</tok> <tok cat="VBN">cloned</tok></cons> <tok cat="NN">receptor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="CD">1</tok> <tok cat="NN">receptor</tok> <tok cat="LRB">(</tok><tok cat="NN">IL-1R</tok><tok cat="RRB">)</tok> <tok cat="NN">superfamily</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">adaptive</tok> <tok cat="NN">immunity</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@35@@oe@16-12-2010
962577003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">Signaling</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">hToll</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">occur</tok> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">sequential</tok> <tok cat="NN">recruitment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">adapter</tok> <tok cat="NN">molecule</tok></cons></cons> <cons cat="NP"><tok cat="NN">MyD88</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">IL-1R-associated</tok> <tok cat="NN">kinase</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
962577004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i8" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="JJ">receptor-activated</tok> <tok cat="NN">factor</tok> <tok cat="CD">6</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TRAF6</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappaB</tok> <tok cat="LRB">(</tok><tok cat="NN">NF-kappaB</tok><tok cat="RRB">)</tok><tok cat="JJ">-inducing</tok> <tok cat="NN">kinase</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NIK</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <tok cat="CC">both</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">subsequent</tok> <tok cat="NNS">steps</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
962577005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Conversely</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">dominant</tok> <tok cat="JJ">negative</tok> <tok cat="NN">version</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TRAF6</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">failed</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">block</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">hToll-induced</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">stress-activated</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase/c-Jun</tok> <tok cat="JJ">NH2-terminal</tok> <tok cat="NNS">kinases</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">early</tok> <tok cat="NN">divergence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="NNS">pathways</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
963276401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">Coactivation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">OCA-B</tok></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">definition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">critical</tok> <tok cat="NNS">regions</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">synergism</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">general</tok> <tok cat="NNS">cofactors</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@16-12-2010
963276402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Molecular</tok> <tok cat="NN">dissection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">B-cell-specific</tok> <tok cat="NN">transcription</tok> <tok cat="NN">coactivator</tok></cons> <cons cat="NP"><tok cat="NN">OCA-B</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">revealed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">distinct</tok> <tok cat="NNS">regions</tok></cons> <cons cat="ADJP"><tok cat="JJ">important</tok><tok cat="COMMA">,</tok> <cons cat="PP" syn="COOD"><cons cat="ADVP"><tok cat="RB">respectively</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">recruitment</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">immunoglobulin</tok> <tok cat="NNS">promoters</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">octamer-bound</tok> <tok cat="NN">Oct-1</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">subsequent</tok> <tok cat="NN">coactivator</tok> <tok cat="NN">function</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@32@@oe@16-12-2010
963276403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Further</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">general</tok> <tok cat="NN">coactivator</tok> <tok cat="NNS">requirements</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">selective</tok> <tok cat="NN">removal</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PC4</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">essential</tok> <tok cat="NN">USA</tok> <tok cat="NN">fraction</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">severely</tok></cons> <cons cat="VP"><tok cat="VBZ">impairs</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Oct-1</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">OCA-B</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">function</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">cell-free</tok> <tok cat="NN">system</tok></cons> <cons cat="VP"><tok cat="VBN">reconstituted</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">partially</tok> <tok cat="VBN">purified</tok></cons> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
963276404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="JJ">Full</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">restored</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">combined</tok> <tok cat="NN">action</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">recombinant</tok> <cons cat="NP"><tok cat="NN">PC4</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">PC4-depleted</tok> <tok cat="NN">USA</tok> <tok cat="NN">fraction</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">joint</tok> <tok cat="NN">requirement</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PC4</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="DT">another</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">USA-derived</tok></cons></cons> <tok cat="NN">component</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">optimal</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Oct-1/OCA-B</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">reconstituted</tok> <tok cat="NN">system</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
963276405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Indeed</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><tok cat="JJ">USA-derived</tok> <tok cat="NN">PC2</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">act</tok> <cons cat="ADVP"><tok cat="RB">synergistically</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">PC4</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">reproducing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">intact</tok> <tok cat="NN">USA</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">assay</tok> <tok cat="NN">system</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
963276406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="JJ">Consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">requirement</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">PC4</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">reconstituted</tok> <tok cat="NN">system</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="NN">OCA-B</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">interact</tok> <cons cat="ADVP"><tok cat="RB">directly</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">PC4</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
963276407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Surprisingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">removal</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PC4</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">unfractionated</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">extract</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="JJ">detrimental</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">OCA-B/Oct-1-dependent</tok> <tok cat="NN">transcription</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
963276408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">lead</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">general</tok> <tok cat="NN">model</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">synergistic</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok> <tok cat="NNS">domains</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Oct-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">OCA-B</tok></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">combined</tok> <tok cat="NN">action</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">multiple</tok> <tok cat="NN">USA</tok> <tok cat="NNS">components</tok></cons></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok><tok cat="COMMA">,</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">further</tok></cons><tok cat="COMMA">,</tok> <tok cat="VBP">suggest</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">functional</tok> <tok cat="NN">redundancy</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">general</tok> <tok cat="NNS">coactivators</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
963382601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Limited</tok> <tok cat="NN">proteolysis</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">assaying</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">ligand</tok> <tok cat="NN">binding</tok> <tok cat="NNS">affinities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@11@@oe@16-12-2010
963382602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">natural</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">synthetic</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">ligands</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">receptors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBG">triggering</tok> <tok cat="NN">event</tok></cons> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><tok cat="NN">gene</tok> <tok cat="NN">transcription</tok> <cons cat="NP"><tok cat="NN">activation</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">repression</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@22@@oe@16-12-2010
963382603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Ligand</tok></cons> <cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">ligand</tok> <tok cat="NN">binding</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">conformational</tok> <tok cat="NNS">changes</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" id="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">evidenced</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="VBN">increased</tok> <tok cat="NN">resistance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NNS">proteases</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
963382604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="FW">In</tok> <tok cat="FW">vitro</tok> <tok cat="VBN">labeled</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">incubated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">various</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">synthetic</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">natural</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" syn="COOD" id="i21"><cons cat="NP"><tok cat="NNS">agonists</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NNS">antagonists</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">submitted</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">trypsin</tok></cons> <tok cat="NN">digestion</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
963382605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="NN">Proteolysis</tok> <tok cat="NNS">products</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">separated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">SDS-PAGE</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">quantified</tok><cons cat="NP" ref="i10" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
963382606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">amount</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">trypsin-resistant</tok> <tok cat="NNS">fragments</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">proportional</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">receptor</tok> <tok cat="NN">occupancy</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="NN">ligand</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">allowed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">determination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">dissociation</tok> <tok cat="NNS">constants</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">kDa</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
963382607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">wild-type</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="VBN">mutated</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="JJ">human</tok> <tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha</tok></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">model</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">kDa</tok> <tok cat="NNS">values</tok></cons> <cons cat="VP"><tok cat="VBN">determined</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">classical</tok> <tok cat="NN">competition</tok> <tok cat="NN">binding</tok> <tok cat="NNS">assays</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="JJ">tritiated</tok> <tok cat="NNS">ligands</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="PP" role="PRD"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">agreement</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="VP"><tok cat="VBN">measured</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="JJ">proteolytic</tok> <tok cat="NN">assay</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
963382608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">method</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">successfully</tok></cons> <cons cat="VP"><tok cat="VBN">extended</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">retinoic</tok> <tok cat="NN">X</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">progesterone</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="VP"><tok cat="VBG">providing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">basis</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">new</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJR">faster</tok></cons></cons> <tok cat="NN">assay</tok></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i19" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="ADVP"><tok cat="RB">simultaneously</tok></cons> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">affinity</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">conformation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">receptors</tok></cons></cons></cons></cons> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i12"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">bound</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">given</tok> <tok cat="NN">ligand</tok></cons></cons><cons cat="ADVP" ref="i12" null="T"></cons></cons></cons></cons><cons cat="ADVP" ref="i19" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
963407501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="FW">Mycobacterium</tok> <tok cat="FW">tuberculosis</tok> <tok cat="JJ">mannose-capped</tok> <tok cat="NN">lipoarabinomannan</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">NF-kappaB-dependent</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok> <cons cat="NP"><tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@16-12-2010
963407502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Tuberculosis</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">emerged</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">epidemic</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">extended</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">large</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">individuals</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">infected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HIV-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@25@@oe@16-12-2010
963407503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">major</tok> <tok cat="NN">goal</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="S" role="PRD"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">mycobacterial</tok> <tok cat="NN">cell</tok></cons> <tok cat="NN">wall</tok> <tok cat="NN">component</tok></cons> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">mannose-capped</tok> <tok cat="NN">lipoarabinomannan</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ManLAM</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="FW">Mycobacterium</tok> <tok cat="FW">tuberculosis</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="FW">M.</tok> <tok cat="FW">tuberculosis</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">use</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">cell</tok> <tok cat="NN">culture</tok> <tok cat="NN">system</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@16-12-2010
963407504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="PP" role="PRD"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">prime</tok> <tok cat="NN">importance</tok></cons></cons> <cons cat="PP"><tok cat="VBG">considering</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">CD4-expressing</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBP">represent</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">major</tok> <tok cat="NN">virus</tok> <tok cat="NN">reservoir</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">infected</tok> <tok cat="NNS">individuals</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
963407505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">1G5</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons> <cons cat="VP"><tok cat="VBG">harbouring</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">luciferase</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">LTR</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">first</tok></cons> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">culture</tok> <tok cat="NN">protein</tok> <tok cat="NNS">filtrates</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">CFP</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="FW">M.</tok> <tok cat="FW">tuberculosis</tok></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="VBN">purified</tok> <tok cat="NN">ManLAM</tok></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="JJ">LTR-dependent</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">unlike</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">similarly</tok> <tok cat="VBN">prepared</tok></cons> <tok cat="NN">CFP</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="ADJP"><tok cat="JJ">devoid</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ManLAM</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@50@@oe@16-12-2010
963407506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">implication</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">A</tok></cons> <tok cat="CC">and/or</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">highlighted</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">abrogation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">ManLAM-mediated</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="JJ">LTR-driven</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">herbimycin</tok> <tok cat="NN">A</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">H7</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
963407507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">determined</tok><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="NN">electrophoresis</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="FW">M.</tok> <tok cat="FW">tuberculosis</tok> <tok cat="NN">ManLAM</tok></cons> <cons cat="VP"><tok cat="VBD">led</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
963407508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="FW">M.</tok> <tok cat="FW">tuberculosis</tok> <tok cat="NN">ManLAM</tok></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">clear</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">luciferase</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="VP"><tok cat="VBN">placed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">wild-type</tok><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <tok cat="RB">not</tok> <tok cat="DT">the</tok> <tok cat="JJ">kappaB-mutated</tok><tok cat="COMMA">,</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">LTR</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
963407509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">ManLAM-mediated</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">LTR</tok></cons> <tok cat="NN">transcription</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">independent</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">autocrine</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">paracrine</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NN">action</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">endogenous</tok> <tok cat="NN">TNF-alpha</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
963407510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="FW">M.</tok> <tok cat="FW">tuberculosis</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">upregulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">potential</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">influence</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathogenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">infection</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
963752901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">IL-4-dependent</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">TGF-alpha</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TGF-beta1</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">eosinophils</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@11@@oe@16-12-2010
963752902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">TGFs</tok></cons> <cons cat="VP"><tok cat="VBP">play</tok> <cons cat="NP"><tok cat="JJ">important</tok> <tok cat="NNS">roles</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">wound</tok> <tok cat="NN">healing</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">carcinogenesis</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@10@@oe@16-12-2010
963752903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">eosinophils</tok></cons> <cons cat="VP"><tok cat="VBG">infiltrating</tok> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">different</tok></cons> <cons cat="ADJP"><tok cat="JJ">pathologic</tok></cons></cons> <tok cat="NNS">processes</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">elaborate</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">TGF-alpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TGF-beta1</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
963752904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NNS">Eosinophils</tok></cons> <cons cat="VP"><tok cat="VBG">infiltrating</tok> <cons cat="NP"><tok cat="NN">hamster</tok> <tok cat="JJ">cutaneous</tok> <tok cat="NNS">wounds</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">express</tok> <cons cat="NP"><tok cat="NNS">TGFs</tok></cons> <cons cat="ADVP"><tok cat="RB">sequentially</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
963752905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">biologic</tok> <tok cat="NNS">mediators</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">TGF-alpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><tok cat="NN">-beta1</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NNS">eosinophils</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
963752906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="NNS">Eosinophils</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">donors</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">cultured</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">absence</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">presence</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-3</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-5</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
963752907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="NNS">Cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">situ</tok></cons> <tok cat="NN">hybridization</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">immunohistochemistry</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
963752908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="NNS">Supernatants</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cultures</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">assayed</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><tok cat="VBN">secreted</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">TGF-alpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TGF-beta1</tok></cons></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="JJ">TGF-specific</tok> <tok cat="NNS">ELISAs</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
963752909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">IL-3</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-5</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">independently</tok></cons> <cons cat="VP"><tok cat="VBD">up-regulated</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">TGF-beta1</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">product</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">eosinophils</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">all</tok> <tok cat="NNS">donors</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
963752910@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Interestingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">TGF-alpha</tok></cons> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NNS">eosinophils</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">up-regulated</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">IL-3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-5</tok></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">down-regulated</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">IL-4</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
963752911@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="JJ">Consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><tok cat="NN">eosinophil</tok> <tok cat="NNS">responses</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-4</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">molecules</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">eosinophils</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
963752912@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">observation</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-4</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">differentially</tok></cons> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">TGF-alpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TGF-beta1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-4</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">serve</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">physiologic</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">switch</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">TGF</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBG">infiltrating</tok> <cons cat="NP"><tok cat="NNS">eosinophils</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">wound</tok> <tok cat="NN">healing</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">carcinogenesis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
964356901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Minimal</tok> <tok cat="JJ">residual</tok> <tok cat="NN">disease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">myelogenous</tok> <tok cat="NN">leukemia</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PML/RAR</tok> <tok cat="NN">alpha</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">AML1/ETO</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">phenotypic</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="JJ">possible</tok> <cons cat="NP"><tok cat="NN">T</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">natural</tok> <tok cat="NN">killer</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">bone</tok> <tok cat="NN">marrow</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@16-12-2010
964356902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBN">studied</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">minimal</tok> <tok cat="JJ">residual</tok> <tok cat="NN">disease</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MRD</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">leukemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AML</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WP">who</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PML/RAR</tok> <tok cat="NN">alpha</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">AML1/ETO</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">phenotypic</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lymphocyte</tok> <tok cat="NNS">subsets</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">antitumor</tok> <tok cat="NN">immunity</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@38@@oe@16-12-2010
964356903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i5" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="CD">Eight</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">long-term</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">LT</tok><tok cat="COLON">;</tok></cons> <cons cat="NP"><cons cat="QP"><tok cat="CD">3</tok> <tok cat="TO">to</tok> <tok cat="CD">15</tok></cons> <tok cat="NNS">years</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" null="QSTN"></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">15</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">short-term</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">ST</tok><tok cat="COLON">;</tok></cons> <cons cat="NP"><cons cat="QP"><tok cat="RB">up</tok> <tok cat="TO">to</tok> <tok cat="CD">3</tok></cons> <tok cat="NN">years</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">remission</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">studied</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
964356904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">reverse</tok> <tok cat="NN">transcription-polymerase</tok> <tok cat="NN">chain</tok> <tok cat="NN">reaction</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RT</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">assay</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">limit</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">detection</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" syn="COOD" role="PRD"><cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">10(-5)</tok> <tok cat="TO">to</tok> <tok cat="CD">10(-6)</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">PML/RAR</tok> <tok cat="NN">alpha</tok> <tok cat="NN">transcript</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">10(-4)</tok> <tok cat="TO">to</tok> <tok cat="CD">10(-5)</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">AML1/ETO</tok> <tok cat="NN">transcript</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
964356905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Simultaneously</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NNS">subsets</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PB</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">bone</tok> <tok cat="NN">marrow</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">BM</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">flow</tok> <tok cat="JJ">cytometric</tok> <tok cat="NN">analysis</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
964356906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="CD">Four</tok> <cons cat="NP" null="QSTN"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">eight</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">LT</tok> <cons cat="NP" null="QSTN"></cons></cons></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">7</tok> <cons cat="NP" null="QSTN"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">15</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">ST</tok> <tok cat="NN">remission</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">MRD-positive</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
964356907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="JJ">MRD-positive</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">LT</tok> <tok cat="NN">remission</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">still</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">until</tok> <cons cat="ADVP"><tok cat="RB">now</tok></cons></cons> <cons cat="ADVP" role="PRD"><tok cat="RB">event-free</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">3</tok> <cons cat="NP" null="QSTN"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">7</tok> <cons cat="ADJP"><cons cat="ADJP"><tok cat="JJ">MRD-positive</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="ADJP"><tok cat="JJ">MRD+</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">ST</tok> <tok cat="NN">remission</tok></cons></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">soon</tok> <cons cat="VP"><tok cat="NN">relapsed</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
964356908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">total</tok> <tok cat="NNS">populations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">CD4+</tok><cons cat="NP" null="QSTN"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">CD8+</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">CD56+</tok> <cons cat="NP" null="QSTN"></cons></cons></cons></cons></cons><cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP" syn="COOD"><tok cat="JJ">possible</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T-cell</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">natural</tok> <tok cat="NN">killer</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">T/NK</tok></cons><tok cat="RRB">)</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NNS">populations</tok></cons></cons><tok cat="RRB">]</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">BM</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">ST</tok> <tok cat="NNS">patients</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">MRD+/LT</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><cons cat="ADVP"><cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">p</tok></cons> <cons cat="ADJP"><tok cat="JJR">&lt;</tok> <cons cat="NP"><tok cat="CD">.01</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="JJ">low</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
964356909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">CD8+</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP"><tok cat="JJ">CD28+</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NN">population</tok></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NN">tendency</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">p</tok></cons> <cons cat="ADJP"><tok cat="JJR">&lt;</tok> <cons cat="NP"><tok cat="CD">.01-.02</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
964356910@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">T/NK</tok> <tok cat="NNS">subsets</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">BM</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><cons cat="ADJP"><cons cat="ADJP"><cons cat="ADJP"><tok cat="JJ">MRD-negative</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="ADJP"><tok cat="JJ">MRD-</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="JJ">LT</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="ADJP"><tok cat="JJ">MRD-/LT</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">similar</tok> <tok cat="NNS">numbers</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">cells</tok></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">normal</tok> <tok cat="NNS">volunteers</tok></cons><cons cat="VP" null="QSTN"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
964356911@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="ADVP"><tok cat="RB">Basically</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">total</tok> <tok cat="NN">percentage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">CD4+</tok><cons cat="NP" ref="i24" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">CD8+</tok> <cons cat="NP" ref="i24" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">CD56+</tok> <cons cat="NP" ref="i24" null="RNR"></cons></cons><cons cat="NP" id="i24"><tok cat="NN">cell</tok> <tok cat="NNS">populations</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">BM</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">increased</tok> <cons cat="NP" ref="i10" null="NONE"></cons></cons></cons><tok cat="CC">and</tok> <cons cat="FRAG"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBG">following</tok> <tok cat="NN">order</tok><cons cat="NP" ref="i30" null="ICH"></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD" id="i30"><cons cat="NP"><tok cat="JJ">MRD-/LT</tok> <tok cat="NNS">patients</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NNS">volunteers</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">MRD+/LT</tok> <tok cat="NNS">patients</tok></cons> <tok cat="CC">and</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">MRD+</tok> <cons cat="NP" ref="i25" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><tok cat="JJ">-/ST</tok> <cons cat="NP" ref="i25" null="RNR"></cons></cons><cons cat="NP" id="i25"><tok cat="NNS">patients</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
964356912@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">percentages</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">T/NK-cell</tok> <tok cat="NNS">subsets</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">PB</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">significantly</tok> <tok cat="JJ">different</tok> <cons cat="PP"><tok cat="IN">among</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">groups</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
964356913@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">difference</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">possible</tok> <cons cat="NP"><tok cat="JJ">T/NK-cell</tok></cons> <tok cat="NN">phenotype</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">BM</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VB">influence</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">clinical</tok></cons> <cons cat="NP" ref="i26" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">molecular</tok></cons> <cons cat="NP" ref="i26" null="RNR"></cons></cons><cons cat="NP" id="i26"><tok cat="NN">remission</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
964356914@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">still</tok></cons> <cons cat="VP"><tok cat="VBP">remain</tok> <cons cat="S"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">confirmed</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">further</tok> <tok cat="NNS">studies</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">functional</tok></cons> <cons cat="ADJP"><tok cat="JJ">anti-tumor</tok></cons></cons> <tok cat="NN">immunity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T/NK</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AML</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">remission</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
964722701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Differential</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Janus</tok> <tok cat="NN">kinase-STAT</tok> <tok cat="NN">pathway</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">biologic</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-13</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">primary</tok> <tok cat="JJ">human</tok> <cons cat="NP"><tok cat="NN">NK</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">comparative</tok> <tok cat="NN">study</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@26@@oe@16-12-2010
964722702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">IL-13</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cytokine</tok></cons> <cons cat="ADJP"><tok cat="JJ">similar</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">regulator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NN">cell</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">monocyte</tok> <tok cat="NNS">functions</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
964722703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="JJ">Biologic</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-13</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">primary</tok> <tok cat="JJ">human</tok> <cons cat="NP"><tok cat="NN">NK</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="ADVP"><tok cat="RB">well</tok></cons> <cons cat="VP"><tok cat="VBN">defined</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
964722704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok><cons cat="S"><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">IL-13</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">IL-4</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">low</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons> <tok cat="NN">secretion</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
964722705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i3"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">costimulated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-13</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons><cons cat="ADVP" ref="i3" null="T"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-13</tok></cons> <cons cat="ADVP"><tok cat="RB">generally</tok></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">types</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">reactivity</tok></cons></cons><cons cat="S" ref="i30" null="ICH"></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="S" syn="COOD" id="i30"><cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="NN">IL-13</tok></cons> <cons cat="VP"><tok cat="VBD">synergized</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">stimulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons> <tok cat="NN">production</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="ADVP"><tok cat="RB">modestly</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="JJ">IL-2-mediated</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons> <tok cat="NN">production</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
964722706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">types</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">donors</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-13</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">IL-2-induced</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons> <tok cat="NN">production</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="PP" role="PRD"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">marked</tok> <tok cat="NN">contrast</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">strong</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="VP"><tok cat="VBN">seen</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-4</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
964722707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Additionally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">IL-13</tok></cons> <cons cat="VP"><tok cat="VBZ">suppresses</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">IL-2-induced</tok> <tok cat="NN">NK</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">cytolytic</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">proliferative</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NNS">activities</tok></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">although</tok> <cons cat="FRAG"><cons cat="ADVP"><tok cat="JJR">less</tok> <tok cat="RB">efficiently</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">than</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-4</tok></cons><cons cat="VP" null="QSTN"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
964722708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">IL-13</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">anti-CD3</tok> <tok cat="NN">mAb/IL-2-</tok> <cons cat="ADJP" null="QSTN"></cons><cons cat="NP" ref="i24" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">PHA-mediated</tok> <cons cat="NP" ref="i24" null="RNR"></cons></cons><cons cat="NP" id="i24"><tok cat="NN">IFN-gamma</tok> <tok cat="NN">production</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">enhances</tok> <cons cat="NP"><tok cat="JJ">cytolytic</tok> <tok cat="NN">potential</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
964722709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">IL-13</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="PP"><tok cat="IN">like</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">distinct</tok> <cons cat="NP"><tok cat="NN">STAT6-DNA</tok> <tok cat="NN">binding</tok> <tok cat="NNS">complexes</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT6</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Janus</tok> <tok cat="NN">kinase</tok> <tok cat="CD">3</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">JAK3</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NK</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
964722710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Abs</tok></cons> <cons cat="VP"><tok cat="VBN">directed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">unique</tok> <tok cat="NNS">domains</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT6</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><tok cat="JJ">differential</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">complexes</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><tok cat="JJ">different</tok> <cons cat="NP"><tok cat="NN">STAT6</tok> <tok cat="NNS">isoforms</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
964722711@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">IL-13</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NK</tok> <cons cat="NP" ref="i27" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <cons cat="NP" ref="i27" null="RNR"></cons></cons><cons cat="NP" id="i27"><tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-</tok><tok cat="CD">13</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potent</tok> <tok cat="NN">regulator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT6</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">JAK3</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
964918601@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Oxidative</tok> <tok cat="NN">stress</tok></cons> <cons cat="VP"><tok cat="VBZ">suppresses</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <tok cat="NNS">activities</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">stimulated</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@10@@oe@16-12-2010
964918602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NNS">Effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">oxidative</tok> <tok cat="NN">stress</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">stimulation-dependent</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">studied</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@16-12-2010
964918603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i16" role="SBJ"><tok cat="VBN">Purified</tok> <tok cat="JJ">quiescent</tok> <tok cat="JJ">human</tok> <tok cat="NN">blood</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">subjected</tok> <cons cat="NP" ref="i16" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok><cons cat="NP" ref="i30" null="ICH"></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD" id="i30"><cons cat="NP"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">i</tok><tok cat="RRB">)</tok></cons> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="NN">exposure</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">hydrogen</tok> <tok cat="NN">peroxide</tok></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">ii</tok><tok cat="RRB">)</tok></cons> <cons cat="NP"><tok cat="JJ">chronic</tok> <tok cat="NN">exposure</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">hydrogen</tok> <tok cat="NN">peroxide</tok></cons></cons></cons><tok cat="COLON">;</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">iii</tok><tok cat="RRB">)</tok></cons> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="NN">exposure</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="VBG">ionizing</tok> <tok cat="NN">radiation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
964918604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">then</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="CD">6</tok> <tok cat="NN">h</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
964918605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NNS">activities</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="VP"><tok cat="VBN">determined</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons><tok cat="COLON">:</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NFkappaB</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NFAT</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">abolished</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="CD">three</tok> <tok cat="NNS">modes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">oxidative</tok> <tok cat="NN">stress</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
964918606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="VP"><tok cat="VBD">exhibited</tok> <cons cat="NP"><tok cat="NN">lipid</tok> <tok cat="NN">peroxidation</tok></cons> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">exposure</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJS">lowest</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">hydrogen</tok> <tok cat="NN">peroxide</tok></cons> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" null="NONE"></cons></cons></cons></cons></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">20</tok> <tok cat="NN">microM</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
964918607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">All</tok> <tok cat="CD">three</tok> <tok cat="NNS">modes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">oxidative</tok> <tok cat="NN">stress</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">catalase</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
964918608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="RB">only</tok> <tok cat="NN">exception</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><cons cat="NP"><tok cat="NN">hydrogen</tok> <tok cat="NN">peroxide</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="CD">20</tok> <tok cat="NN">microM</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">catalase</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
964918609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok><cons cat="S" syn="COOD"><tok cat="COLON">:</tok> <cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">i</tok><tok cat="RRB">)</tok></cons> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">suppression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <tok cat="NNS">functions</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">potentially</tok></cons> <cons cat="VP"><tok cat="VB">serve</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">marker</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">exposure</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">oxidative</tok> <tok cat="NN">stress</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">ii</tok><tok cat="RRB">)</tok></cons> <cons cat="NP" role="SBJ"><tok cat="NN">lipid</tok> <tok cat="NN">peroxidation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">only</tok> <tok cat="JJ">detectable</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">exposure</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">weak</tok> <tok cat="JJ">oxidative</tok></cons> <tok cat="NN">stress</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">catalase</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">iii</tok><tok cat="RRB">)</tok></cons> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <tok cat="JJ">DNA-binding</tok> <tok cat="NNS">activities</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="RBR">more</tok> <tok cat="JJ">sensitive</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">oxidative</tok> <tok cat="NN">stress</tok></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">than</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">lipid</tok> <tok cat="NN">peroxidation</tok></cons><cons cat="ADJP" null="QSTN"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@74@@oe@16-12-2010
964934101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">Ro</tok> <tok cat="CD">09-2210</tok></cons> <cons cat="VP"><tok cat="VBZ">exhibits</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">potent</tok></cons> <cons cat="ADJP"><tok cat="JJ">anti-proliferative</tok></cons></cons> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">selectively</tok></cons> <cons cat="VP"><tok cat="VBG">blocking</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">MKK</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@16-12-2010
964934102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NN">throughput</tok> <tok cat="NN">screening</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">microbial</tok> <tok cat="NNS">broths</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">compound</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">designated</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NN">Ro</tok> <tok cat="CD">09-2210</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">block</tok> <cons cat="NP"><cons cat="ADJP"><cons cat="NP"><tok cat="JJ">anti-CD3</tok></cons> <tok cat="JJ">induced</tok></cons> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">IC50</tok></cons> <cons cat="ADJP"><tok cat="JJ">=</tok> <cons cat="NP"><tok cat="CD">40</tok> <tok cat="NN">nM</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@38@@oe@16-12-2010
964934103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="NN">Ro</tok> <tok cat="CD">09-2210</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">block</tok> <cons cat="NP"><tok cat="JJ">antigen-induced</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="NN">secretion</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">IC50</tok></cons> <cons cat="ADJP"><tok cat="JJ">=</tok> <cons cat="NP"><tok cat="CD">30</tok> <tok cat="NN">nM</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="ADVP"><tok cat="RB">considerably</tok> <tok cat="RBR">less</tok></cons> <tok cat="JJ">potent</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">blocking</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Ca2+</tok> <tok cat="NN">flux</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">anti-CD3</tok></cons> <tok cat="NN">treatment</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
964934104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">action</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Ro</tok> <tok cat="CD">09-2210</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">set</tok> <cons cat="PRT"><tok cat="IN">up</tok></cons> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">transient</tok> <tok cat="NN">expression</tok> <tok cat="NN">system</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">variety</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">reporter</tok> <tok cat="NN">gene</tok> <tok cat="NNS">constructs</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">effective</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">phorbol</tok> <tok cat="JJ">ester/ionomycin-induced</tok></cons> <cons cat="NP"><tok cat="NN">NF-AT</tok></cons> <tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">anti-CD3</tok> <tok cat="VBD">induced</tok></cons> <cons cat="NP"><tok cat="NN">NF-AT</tok></cons> <cons cat="NP" null="QSTN"></cons></cons></cons></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IC50</tok></cons> <cons cat="ADJP"><tok cat="JJ">=</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">7.7</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">10</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">nM</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@16-12-2010
964934105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="NN">Ro</tok> <tok cat="CD">09-2210</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">phorbol</tok> <tok cat="JJ">ester/ionomycin-induced</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AP1</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IC50</tok></cons> <cons cat="ADJP"><tok cat="JJ">=</tok> <tok cat="JJR">&lt;</tok><cons cat="NP"><tok cat="CD">10</tok> <tok cat="NN">nM</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
964934106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">further</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="NN">Ro</tok> <tok cat="CD">09-2210</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unable</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><cons cat="NP"><tok cat="NN">c-jun</tok></cons> <tok cat="JJ">induced</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">AP1-dependent</tok> <tok cat="NN">reporter</tok> <tok cat="NNS">constructs</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">IC50</tok></cons> <cons cat="ADJP"><tok cat="JJR">&gt;</tok> <cons cat="NP"><tok cat="CD">500</tok> <tok cat="NN">nM</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="ADVP"><tok cat="RB">potently</tok></cons> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><tok cat="JJ">ras-induced</tok> <cons cat="NP"><tok cat="NN">AP1</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">IC50</tok></cons> <cons cat="ADJP"><tok cat="JJ">=</tok> <cons cat="NP"><tok cat="CD">20</tok> <tok cat="NN">nM</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
964934107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBD">suggested</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Ro</tok> <tok cat="CD">09-2210</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBG">inhibiting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">activator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AP-1</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i12"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i12" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP" syn="COOD"><cons cat="ADVP"><tok cat="JJ">upstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-jun</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">downstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">ras</tok></cons> <tok cat="NN">signaling</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
964934108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i14" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">investigate</tok> <cons cat="ADVP"><tok cat="RB">further</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i14" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="ADVP"><tok cat="RB">then</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">purified</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">kinases</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PKC</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">PhK</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">ZAP-70</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">ERK</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">MEK</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">a</tok> <cons cat="NP"><tok cat="NN">MKK</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Ro</tok> <tok cat="CD">09-2210</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">selective</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MEK1</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">IC50</tok></cons> <cons cat="ADJP"><tok cat="JJ">=</tok> <cons cat="NP"><tok cat="CD">59</tok> <tok cat="NN">nM</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@50@@oe@16-12-2010
965772001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">A</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">small</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">nonpeptidyl</tok></cons></cons> <tok cat="NN">mimic</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">granulocyte-colony-stimulating</tok> <tok cat="NN">factor</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VB">see</tok> <cons cat="NP"><tok cat="NNS">commetns</tok></cons></cons></cons><tok cat="RRB">]</tok></cons></cons></cons>@@@@1@12@@oe@16-12-2010
965772002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">A</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">nonpeptidyl</tok></cons> <cons cat="ADJP"><tok cat="JJ">small</tok></cons></cons> <tok cat="NN">molecule</tok></cons> <cons cat="NP"><tok cat="NN">SB</tok> <tok cat="CD">247464</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">activating</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">granulocyte-colony-stimulating</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">G-CSF</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok> <tok cat="NNS">pathways</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">high-throughput</tok> <tok cat="NN">assay</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">cultured</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@29@@oe@16-12-2010
965772003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Like</tok> <cons cat="NP"><tok cat="NN">G-CSF</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">SB</tok> <tok cat="CD">247464</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">multiple</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">murine</tok> <tok cat="NN">bone</tok> <tok cat="NN">marrow</tok> <tok cat="NNS">cells</tok></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">form</tok> <cons cat="NP"><tok cat="JJ">granulocytic</tok> <tok cat="NNS">colonies</tok></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
965772004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="JJ">elevated</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">neutrophil</tok></cons> <tok cat="NNS">counts</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">mice</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
965772005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">extracellular</tok> <tok cat="NN">domain</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">G-CSF</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SB</tok> <tok cat="CD">247464</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">compound</tok></cons> <cons cat="VP"><tok cat="VBZ">acts</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">oligomerizing</tok> <cons cat="NP"><tok cat="NN">receptor</tok> <tok cat="NNS">chains</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
965772006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">a</tok> <tok cat="JJ">small</tok> <tok cat="NN">molecule</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">receptor</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">normally</tok></cons> <cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">relatively</tok> <tok cat="JJ">large</tok></cons> <tok cat="NN">protein</tok> <tok cat="NN">ligand</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
965774201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">proliferation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">definitive</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">megakaryocytic</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@16-12-2010
965774202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">elucidate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">contributions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">definitive</tok> <tok cat="NN">hematopoiesis</tok></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="NNS">mice</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" id="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">rendered</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="ADJP" role="PRD"><tok cat="RB">genetically</tok> <tok cat="JJ">defective</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GATA-1</tok></cons> <tok cat="NN">synthesis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNP">Takahashi</tok> <tok cat="FW">et</tok> <tok cat="FW">al</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">J</tok> <tok cat="NNP">Biol</tok> <tok cat="NNP">Chem</tok> <tok cat="CD">272</tok></cons><tok cat="COLON">:</tok><cons cat="NP"><tok cat="CD">12611</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">1997</tok></cons></cons><tok cat="RRB">)</tok></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@40@@oe@16-12-2010
965774203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Because</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">X</tok> <tok cat="NN">chromosome</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" id="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">randomly</tok></cons> <cons cat="VP"><tok cat="VBN">inactivated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">every</tok> <tok cat="NN">cell</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">heterozygous</tok> <tok cat="NNS">females</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">bear</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <tok cat="DT">an</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">active</tok> <tok cat="JJ">wild-type</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">mutant</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="VP"><tok cat="VBN">referred</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" null="NONE"></cons></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">GATA-1.05</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" id="i21"><cons cat="NP"><tok cat="NN">GATA-1</tok></cons> <tok cat="NN">allele</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">consequently</tok></cons> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">variable</tok></cons> <cons cat="ADJP"><tok cat="JJ">anemic</tok></cons></cons> <tok cat="NN">severity</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
965774204@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="JJ">heterozygous</tok> <tok cat="JJ">mutant</tok> <tok cat="NNS">mice</tok></cons> <cons cat="ADVP"><tok cat="RB">usually</tok></cons> <cons cat="VP"><tok cat="VBN">developed</tok> <cons cat="ADVP"><tok cat="RB">normally</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBD">began</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">die</tok> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="CD">5</tok> <tok cat="NNS">months</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
965774205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="VBN">affected</tok> <tok cat="NNS">animals</tok></cons> <cons cat="VP"><tok cat="VBD">displayed</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">marked</tok> <cons cat="NP"><tok cat="NN">splenomegaly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">anemia</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">thrombocytopenia</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
965774206@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NNS">Proerythroblasts</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">megakaryocytes</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">massively</tok></cons> <cons cat="VP"><tok cat="VBN">accumulated</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">spleens</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">heterozygotes</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">neomycin</tok> <tok cat="NN">resistance</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">positive</tok> <tok cat="NN">selection</tok> <tok cat="NN">marker</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">ES</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">profusely</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">abnormally</tok> <tok cat="JJ">abundant</tok></cons> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">generated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GATA-1.05</tok></cons> <tok cat="JJ">mutant</tok> <tok cat="NNS">females</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@16-12-2010
965774207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">hematopoiesis</tok></cons> <cons cat="PP"><tok cat="NN">outside</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">bone</tok> <tok cat="NN">marrow</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">affected</tok> <tok cat="JJ">mutant</tok> <tok cat="NNS">mice</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
965774208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">small</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GATA-1.05</tok> <tok cat="JJ">mutant</tok> <tok cat="JJ">hematopoietic</tok> <tok cat="NN">progenitor</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">begin</tok> <cons cat="S"><cons cat="NP" ref="i14" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">proliferate</tok> <cons cat="ADVP"><tok cat="RB">vigorously</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">adulthood</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">because</tok> <cons cat="S"><cons cat="NP" id="i15" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unable</tok> <cons cat="S"><cons cat="NP" ref="i15" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="ADVP"><tok cat="RB">terminally</tok></cons> <cons cat="VP"><tok cat="VB">differentiate</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok></cons> <cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">progenitor</tok> <tok cat="NN">proliferation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">spleen</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">consequently</tok></cons> <cons cat="NP"><tok cat="NN">death</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
965774209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">GATA-1</tok></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">important</tok></cons> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons> <tok cat="NNS">roles</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">directing</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">definitive</tok> <cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NNS">progenitors</tok></cons></cons> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">differentiate</tok> <cons cat="PP"><tok cat="IN">along</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <tok cat="DT">the</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">erythroid</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">megakaryocytic</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NNS">pathways</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
965774210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok></cons> <tok cat="JJ">heterozygous</tok> <tok cat="JJ">mutant</tok> <tok cat="NN">mouse</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">shows</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">phenotype</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i16"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i16" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">analogous</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">myelodysplastic</tok> <tok cat="NN">syndrome</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">serve</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">useful</tok> <tok cat="NN">model</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">disorder</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
965774301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Erythropoietin</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Jak2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">STAT5A</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT5B</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="VBN">cultured</tok> <tok cat="JJ">human</tok> <tok cat="JJ">erythroid</tok> <tok cat="NNS">precursors</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@16-12-2010
965774302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">signaling</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">erythropoietin</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="RB">highly</tok> <tok cat="VBN">purified</tok> <tok cat="JJ">human</tok> <tok cat="NN">colony</tok> <tok cat="NN">forming</tok> <tok cat="JJ">unit-erythroid</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">generated</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">CD34(+)</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@16-12-2010
965774303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">erythropoietin</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Jak2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">STAT5A</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT5B</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
965774304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Jak2</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">reaches</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">peak</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">around</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">10</tok> <tok cat="NNS">minutes</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">maximum</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">5</tok> <tok cat="NN">U/mL</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">erythropoietin</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
965774305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">Tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT5</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">accompanied</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <cons cat="NP"><tok cat="NN">STAT5</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EMSA</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">32Pi-labeled</tok> <cons cat="NP"><tok cat="NN">STAT5</tok> <tok cat="NN">binding</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">beta-casein</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
965774306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">Tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT1</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">STAT3</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">erythroid</tok> <tok cat="NNS">precursors</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">erythropoietin</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
965774307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">Crkl</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="NN">SH2/SH3</tok> <tok cat="NN">adapter</tok> <tok cat="NN">protein</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">becomes</tok> <cons cat="VP"><tok cat="VBN">coimmunoprecipitated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">STAT5</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">erythropoietin-stimulated</tok> <tok cat="JJ">erythroid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><cons cat="SBAR" ref="i30" func="ADV" null="ICH"></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="SBAR" id="i30" func="ADV"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">become</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">c-Cbl</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
965774308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i11" role="SBJ"><tok cat="JJ">human</tok> <tok cat="JJ">erythroid</tok> <tok cat="NNS">precursors</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">expanded</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">sufficient</tok> <cons cat="NP"><tok cat="NNS">numbers</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">purity</tok></cons></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">allow</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">usage</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">transduction</tok> <tok cat="NNS">studies</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
965774309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">report</tok></cons> <cons cat="VP"><tok cat="VBZ">sets</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">basis</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">further</tok> <tok cat="NNS">studies</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">signaling</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="VBN">cultured</tok> <tok cat="JJ">human</tok> <tok cat="JJ">erythroid</tok> <tok cat="NNS">precursors</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i17"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i17" role="SBJ" null="T"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">turn</tok></cons></cons> <cons cat="VP"><tok cat="VBP">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">our</tok> <tok cat="JJR">better</tok> <tok cat="NN">understanding</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">differentiation</tok> <tok cat="NNS">processes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">erythrocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NNS">precursors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
965774501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">novel</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">Stat1</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Stat3</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NNS">proteins</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">erythropoietin-induced</tok> <tok cat="JJ">erythroid</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
965774502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP"><tok cat="VBD">determined</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">erythropoietin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EPO</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">3</tok> <tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <tok cat="NN">signal</tok> <cons cat="NP"><tok cat="NN">transducer</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">activator</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">STAT</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">family</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Stat1alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Stat3</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Stat5</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">EPO-dependent</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">UT-7</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">UT-7/EPO</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NNP">Kirito</tok> <tok cat="FW">et</tok> <tok cat="FW">al</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">J</tok> <tok cat="NNP">Biol</tok> <tok cat="NNP">Chem</tok> <tok cat="CD">272</tok></cons><tok cat="COLON">:</tok><cons cat="NP"><tok cat="CD">16507</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">1997</tok></cons></cons><tok cat="RRB">)</tok></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@56@@oe@16-12-2010
965774503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">Stat1alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">Stat3</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">EPO-induced</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">proliferation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
965774504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">roles</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Stat1alpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Stat3</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">EPO-induced</tok> <tok cat="JJ">erythroid</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
965774505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">UT-7/GM</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">model</tok> <tok cat="NN">system</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">because</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">differentiate</tok> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="JJ">erythroid-lineage</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">EPO</tok></cons> <tok cat="NN">treatment</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">Komatsu</tok> <tok cat="FW">et</tok> <tok cat="FW">al</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Blood</tok> <tok cat="CD">89</tok></cons><tok cat="COLON">:</tok><cons cat="NP"><tok cat="CD">4021</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">1997</tok></cons></cons><tok cat="RRB">)</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
965774506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">EPO</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Stat1alpha</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">Stat3</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">UT-7/GM</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
965774507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Transfection</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">Stat1alpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Stat3</tok></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">EPO</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">gamma-globin</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">erythroid-specific</tok> <tok cat="JJ">5-aminolevulinate</tok> <tok cat="NN">synthetase</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">transcripts</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">reduction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">percentage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">hemoglobin-positive</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
965774508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Dominant</tok> <tok cat="JJ">negative</tok> <tok cat="NNS">forms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Stat1alpha</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">Stat3</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">promoted</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">EPO-induced</tok> <tok cat="JJ">erythroid</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">UT-7/GM</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">even</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">granulocyte-macrophage</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">cytokine</tok></cons> <cons cat="ADVP"><tok cat="RB">never</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">parent</tok> <tok cat="NN">UT-7/GM</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">EPO</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
965774509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">A</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">constitutive</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Stat1alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">Stat3</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">shortened</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">period</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">G0/G1</tok> <tok cat="NN">prolongation</tok></cons> <cons cat="VP"><tok cat="VBN">caused</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">EPO</tok></cons> <tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
965774510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">Taken</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">together</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Stat1alpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Stat3</tok></cons></cons> <cons cat="VP"><tok cat="VBP">act</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="JJ">negative</tok> <tok cat="NNS">regulators</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">EPO-induced</tok> <tok cat="JJ">erythroid</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
965774511@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Specifically</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">Stat1alpha</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="JJ">cycle-associated</tok> <tok cat="NN">gene</tok></cons><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">entry</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
966093801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">DNA</tok> <tok cat="NN">damaging</tok> <tok cat="NNS">agents</tok></cons> <cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Fas</tok> <tok cat="NN">ligand</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">subsequent</tok> <tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@16-12-2010
966093802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Apoptosis</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">damage</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">stresses</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">proceed</tok> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Fas</tok> <tok cat="NN">ligand</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">FasL</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">ligation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">receptor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Fas</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CD95</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@29@@oe@16-12-2010
966093803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">crucially</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">FasL</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">etoposide</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">teniposide</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">UV</tok> <tok cat="NN">irradiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
966093804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">nondegradable</tok> <tok cat="NN">mutant</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">blocked</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">FasL</tok></cons> <tok cat="NN">expression</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">DNA</tok> <tok cat="NN">damage</tok></cons></cons></cons></cons></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">Fas</tok></cons> <tok cat="NN">ligation</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
966093805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">stimuli</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">stress-activated</tok> <tok cat="NN">kinase</tok> <tok cat="NN">pathway</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SAPK/JNK</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" id="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">maximal</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
966093806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">A</tok> <cons cat="ADJP"><tok cat="CD">1.2</tok> <tok cat="NN">kb</tok></cons> <cons cat="NP"><tok cat="NN">FasL</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="VP"><tok cat="VBD">responded</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">damage</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">coexpression</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p65</tok> <tok cat="NN">Rel</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">Fos/Jun</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
966093807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Mutations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <tok cat="JJ">relevant</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">eliminated</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">responses</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
966093808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">contributes</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">stress-induced</tok> <tok cat="NN">apoptosis</tok></cons></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">FasL</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
966346701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Thrombopoietin</tok></cons> <cons cat="VP"><tok cat="VBZ">supports</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="JJ">erythroid</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">specific</tok> <tok cat="NN">receptor</tok> <tok cat="NN">c-Mpl</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">human</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
966346702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Thrombopoietin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TPO</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">acts</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">megakaryopoiesis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">erythropoiesis</tok></cons></cons></cons> <cons cat="ADVP" syn="COOD"><cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
966346703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">isolated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="NN">subline</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">UT-7/GMT</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">UT-7/GM</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">N.</tok> <tok cat="NN">Komatsu</tok></cons><tok cat="COMMA">,</tok> <tok cat="FW">et</tok> <cons cat="NP"><tok cat="FW">al.</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Blood</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">89</tok><tok cat="COLON">:</tok> <tok cat="CD">4021-4033</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" adv="TMP"><tok cat="CD">1997</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
966346704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">small</tok> <tok cat="NN">population</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">UT-7/GM</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">positively</tok></cons> <tok cat="VBN">stained</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">hemoglobin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Hb</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">7-day</tok> <tok cat="NN">exposure</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">TPO</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
966346705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="QP"><tok cat="JJR">More</tok> <tok cat="IN">than</tok> <tok cat="CD">50</tok></cons><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">TPO-treated</tok> <tok cat="NN">UT-7/GMT</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">positively</tok></cons> <tok cat="VBN">stained</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">Hb</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
966346706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><tok cat="NN">UT-7/GMT</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i1" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="SBAR"><cons cat="WHADVP" id="i2"><tok cat="WRB">how</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">TPO</tok></cons> <cons cat="VP"><tok cat="VBZ">promotes</tok> <cons cat="NP"><tok cat="NN">hemoglobinization</tok></cons><cons cat="ADVP" ref="i2" null="T"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
966346707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">TPO</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">TPO</tok> <tok cat="NN">receptor</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">erythropoietin</tok> <tok cat="LRB">(</tok><tok cat="NN">EPO</tok><tok cat="RRB">)</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
966346708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">There</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">competition</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">TPO</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">EPO</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">EPO</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
966346709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">TPO</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">direct</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">hemoglobinization</tok></cons></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">specific</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">UT-7/GMT</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
966346710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Isoelectric</tok> <tok cat="NN">focusing</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">TPO</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">fetal</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">adult</tok></cons></cons> <tok cat="NN">Hb</tok> <tok cat="NN">synthesis</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">EPO</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">embryonic</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">fetal</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">adult</tok></cons></cons> <tok cat="NN">Hb</tok> <tok cat="NN">synthesis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
966346711@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">TPO</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">distinct</tok> <tok cat="NN">action</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">erythropoiesis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
966546001@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Macrophages</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">atheroma</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">contain</tok> <cons cat="NP"><tok cat="NN">PPARgamma</tok></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">differentiation-dependent</tok> <tok cat="JJ">peroxisomal</tok> <tok cat="JJ">proliferator-activated</tok> <tok cat="NN">receptor</tok> <tok cat="NN">gamma</tok></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PPARgamma</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">expression</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">reduction</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">MMP-9</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PPARgamma</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NNS">phagocytes</tok></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@30@@oe@16-12-2010
966546002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Mononuclear</tok> <tok cat="NNS">phagocytes</tok></cons> <cons cat="VP"><tok cat="VBP">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">atherosclerosis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">sequela</tok> <tok cat="NN">plaque</tok> <tok cat="NN">rupture</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">part</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">secretion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">matrix</tok> <tok cat="NNS">metalloproteinases</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">MMPs</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><tok cat="NN">MMP-9</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
966546003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Peroxisomal</tok> <tok cat="JJ">proliferator-activated</tok> <tok cat="NN">receptor</tok> <tok cat="NN">gamma</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PPARgamma</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">receptor</tok> <tok cat="NN">superfamily</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">regulates</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NNS">activators</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">15-deoxy-delta12,14-prostaglandin</tok> <tok cat="NN">J2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">antidiabetic</tok> <tok cat="NN">agent</tok> <tok cat="NN">troglitazone</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
966546004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PPARgamma</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">atherosclerosis</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unexplored</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
966546005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">monocytes/macrophages</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">atherosclerotic</tok> <tok cat="NNS">lesions</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">n</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">12</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP"><tok cat="JJ">immunostainable</tok> <tok cat="NN">PPARgamma</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
966546006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Normal</tok> <tok cat="NN">artery</tok> <tok cat="NNS">specimens</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">n</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">6</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBP">reveal</tok> <cons cat="NP"><tok cat="JJ">minimal</tok> <tok cat="JJ">immunoreactive</tok> <tok cat="NN">PPARgamma</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
966546007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">Human</tok> <tok cat="NNS">monocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">monocyte-derived</tok> <tok cat="NNS">macrophages</tok></cons></cons> <cons cat="VP"><tok cat="VBN">cultured</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="CD">6</tok> <tok cat="NNS">days</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">5</tok><tok cat="NN">%</tok></cons> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">serum</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PPARgamma</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">reverse</tok> <tok cat="NN">transcription-polymerase</tok> <tok cat="NN">chain</tok> <tok cat="NN">reaction</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Western</tok> <tok cat="NN">blotting</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
966546008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">PPARgamma</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">increased</tok> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">phorbol</tok> <tok cat="NN">12-myristate</tok> <tok cat="CD">13</tok> <tok cat="JJ">acetate-induced</tok></cons> <tok cat="NN">differentiation</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
966546009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PPARgamma</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">troglitazone</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">15-deoxy-delta12,14-prostaglandin</tok> <tok cat="NN">J2</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">human</tok> <tok cat="JJ">monocyte-derived</tok></cons> <tok cat="NNS">macrophages</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="NN">MMP-9</tok> <tok cat="JJ">gelatinolytic</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">concentration-dependent</tok> <tok cat="NN">fashion</tok></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">revealed</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">zymography</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
966546010@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="VP"><tok cat="VBZ">correlates</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="VBN">decreased</tok> <tok cat="NN">MMP-9</tok> <tok cat="NN">secretion</tok></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">determined</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">Western</tok> <tok cat="NN">blotting</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
966546011@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">PPARgamma</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">macrophages</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">atherosclerotic</tok> <tok cat="NNS">lesions</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">expression</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">MMP-9</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">enzyme</tok></cons> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">plaque</tok> <tok cat="NN">rupture</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
966546012@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="NN">PPARgamma</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">likely</tok> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">regulator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">monocyte/macrophage</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">relevance</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">atherosclerotic</tok> <tok cat="NN">disease</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
966588401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Uncoupling</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nonreceptor</tok> <tok cat="NN">tyrosine</tok> <tok cat="NNS">kinases</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PLC-gamma1</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">SLP-76-deficient</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
966588402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nonreceptor</tok> <tok cat="NN">protein</tok> <tok cat="NN">tyrosine</tok> <tok cat="NNS">kinases</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PTKs</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><tok cat="NN">TCR</tok><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">responsiveness</tok></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">individual</tok> <tok cat="NN">PTK</tok> <tok cat="NNS">substrates</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">often</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">uncertain</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@32@@oe@16-12-2010
966588403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">mutant</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="SBAR" ref="i2" null="ICH"></cons></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="SBAR" id="i2"><cons cat="WHNP" id="i3"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">lacked</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">SLP-76</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">SH2</tok> <tok cat="JJ">domain-containing</tok> <tok cat="NN">leukocyte</tok> <tok cat="NN">protein</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">76</tok> <tok cat="NNS">kilodaltons</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="RB">hematopoietically</tok> <tok cat="VBN">expressed</tok> <tok cat="NN">adaptor</tok> <tok cat="NN">protein</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">PTK</tok> <tok cat="NN">substrate</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
966588404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="NN">SLP-76</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">TCR-induced</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJS">most</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">optimal</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phospholipase</tok> <tok cat="NN">C-gamma1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PLC-gamma1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><tok cat="NN">Ras</tok> <tok cat="NN">pathway</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
966588405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP"><tok cat="JJ">TCR-inducible</tok></cons> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">SLP-76</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@16-12-2010
966588406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">coupling</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">TCR-regulated</tok> <tok cat="NN">PTKs</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">downstream</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP"><tok cat="NN">SLP-76</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
967676101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">Peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">lupus</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">estrogen</tok> <tok cat="NN">receptor</tok> <tok cat="NNS">transcripts</tok></cons> <cons cat="ADJP"><tok cat="JJ">similar</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
967676102@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">OBJECTIVE</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">identify</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">characterize</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><cons cat="NP" id="i1"><cons cat="NP"><tok cat="NN">estrogen</tok> <tok cat="NN">receptor</tok> <tok cat="LRB">(</tok><tok cat="NN">ER</tok><tok cat="RRB">)</tok> <tok cat="NNS">transcripts</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">immune</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">systemic</tok> <tok cat="NN">lupus</tok> <tok cat="NN">erythematosus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SLE</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">donors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@29@@oe@16-12-2010
967676103@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">METHODS</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i2" role="SBJ"><cons cat="NP"><tok cat="JJ">Peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">monocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">prepared</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">SLE</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">n</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">6</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">donors</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">n</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">8</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
967676104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">separated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CD4</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CD8</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
967676105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="DT">Some</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">estradiol</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">PMA</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">ionomycin</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
967676106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="JJ">virus-transformed</tok></cons> <tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">n</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">7</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NNS">hybridomas</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">n</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="LS">2</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">established</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">SLE</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">healthy</tok> <tok cat="JJ">individual</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="RB">source</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
967676107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">ER</tok> <tok cat="NN">mRNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">reverse</tok> <tok cat="NN">transcription</tok> <tok cat="JJ">nested</tok></cons> <tok cat="NN">polymerase</tok> <tok cat="NN">chain</tok> <tok cat="NN">reaction</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
967676108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="JJ">Amplified</tok> <tok cat="NN">cDNA</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">sequenced</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">standard</tok> <tok cat="NNS">methods</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@16-12-2010
967676109@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">RESULTS</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">tested</tok><cons cat="NP" null="NONE"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><tok cat="NN">ER</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP"><tok cat="JJ">prior</tok> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
967676110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Partial</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NNS">exons</tok> <tok cat="CD">1-8</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">nearly</tok> <tok cat="JJ">identical</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">published</tok> <tok cat="NN">sequence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">ER</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
967676111@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">There</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="NP" role="PRD"><tok cat="DT">no</tok> <tok cat="JJ">notable</tok> <tok cat="NNS">differences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ER</tok> <tok cat="NNS">transcripts</tok></cons></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">controls</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
967676112@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="JJ">Variant</tok> <tok cat="NN">receptor</tok> <tok cat="NNS">transcripts</tok></cons> <cons cat="VP"><tok cat="VBG">lacking</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">exon</tok> <tok cat="CD">5</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">exon</tok> <tok cat="CD">7</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">encodes</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">hormone</tok> <tok cat="NN">binding</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">majority</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
967676113@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Precise</tok> <tok cat="NN">deletion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">exons</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="ADJP"><tok cat="RB">alternatively</tok> <tok cat="VBN">spliced</tok></cons> <tok cat="NNS">transcripts</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
967676114@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" id="i11" role="SBJ"><tok cat="IN">Whether</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">detected</tok> <tok cat="NNS">transcripts</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">translated</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="NN">receptor</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">remains</tok> <cons cat="S"><cons cat="NP" ref="i11" id="i13" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">determined</tok><cons cat="NP" ref="i13" null="NONE"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
967676115@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP"><tok cat="FW">In</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">stimulation</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><tok cat="NN">ER</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
967676116@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">variants</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">correlate</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">disease</tok> <tok cat="NN">activity</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">medication</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
967676117@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">CONCLUSION</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">Monocytes</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">patients</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">transcripts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">normal</tok> <tok cat="JJ">wild</tok> <tok cat="NN">type</tok> <tok cat="NN">ER</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">hormone</tok> <tok cat="NN">binding</tok> <tok cat="NN">domain</tok> <tok cat="NNS">variants</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
967871801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">A</tok> <tok cat="JJ">novel</tok> <cons cat="ADJP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RAR</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="JJ">-selective</tok></cons> <tok cat="NN">antagonist</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HL-60</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">implications</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">RARalpha-mediated</tok></cons> <tok cat="NNS">signals</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="JJ">leukemic</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@16-12-2010
967871802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Retinoic</tok> <tok cat="NN">acid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RA</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HL-60</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">differentiate</tok> <cons cat="ADVP"><tok cat="RB">terminally</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="NNS">granulocytes</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="ADVP" adv="TMP"><tok cat="RB">subsequently</tok></cons> <cons cat="VP"><tok cat="VBP">die</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
967871803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">biological</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">RA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">distinct</tok> <tok cat="NNS">families</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">RARs</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">retinoid</tok> <tok cat="NN">X</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">RXRs</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
967871804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NNS">RARs</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">RXRs</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="NN">form</tok> <cons cat="NP"><tok cat="NNS">heterodimers</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">regulate</tok> <cons cat="NP"><tok cat="JJ">retinoid-mediated</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
967871805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP"><tok cat="VBN">developed</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <cons cat="ADJP"><tok cat="JJ">RAR-selective</tok></cons> <tok cat="NN">antagonist</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ER27191</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">prevents</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">RAR</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NNS">retinoids</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
967871806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">RAR-selective</tok> <tok cat="NN">antagonist</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">RXR</tok> <tok cat="CC">and</tok> <tok cat="NN">RAR</tok> <tok cat="NN">agonist</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><cons cat="COMP" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">RAR-mediated</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="JJ">leukemic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
967871807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Simple</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">RXRs</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">sufficient</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
967871808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Interestingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">RAR-selective</tok> <tok cat="NN">antagonist</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">9-cis</tok> <tok cat="NN">RA</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">partial</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HL-60</tok> <tok cat="CC">and</tok> <tok cat="NN">NB4</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">RAR</tok> <tok cat="NN">antagonist</tok></cons> <cons cat="ADVP"><tok cat="RB">completely</tok></cons> <cons cat="VP"><tok cat="VBD">blocked</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">all-trans</tok> <tok cat="JJ">RA-induced</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
967871809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Additional</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">BCL-2</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">decreased</tok> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="JJ">leukemic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
967871810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">HL-60</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">transduced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">bcl-2</tok> <tok cat="NN">expression</tok> <tok cat="NN">vector</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NN">differentiation</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NNS">retinoids</tok></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="SINV"><tok cat="VBD">did</tok> <cons cat="NP" role="SBJ"><tok cat="JJ">parental</tok> <tok cat="NN">HL-60</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP" null="QSTN"></cons></cons></cons><cons cat="SBAR" func="ADV"><tok cat="RB">even</tok> <tok cat="IN">though</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="NN">apoptosis</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="JJ">bcl-2-transduced</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">independently</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="JJ">leukemic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
967982901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Suppression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">anti-porcine</tok> <tok cat="NN">T-cell</tok> <tok cat="JJ">immune</tok> <tok cat="NNS">responses</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">major</tok> <tok cat="NN">histocompatibility</tok> <tok cat="NN">complex</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">transactivator</tok> <tok cat="NNS">constructs</tok></cons> <cons cat="VP"><tok cat="VBG">lacking</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">amino</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
967982902@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">BACKGROUND</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">transactivator</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CIITA</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">bi-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">multifunctional</tok></cons></cons> <tok cat="NN">domain</tok> <tok cat="NN">protein</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">acts</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activator</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">critical</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@36@@oe@16-12-2010
967982903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="VBN">mutated</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">CIITA</tok> <tok cat="NN">gene</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">coding</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBG">lacking</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">amino</tok> <tok cat="JJ">terminal</tok> <tok cat="CD">151</tok> <tok cat="NN">amino</tok> <tok cat="NNS">acids</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">acts</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potent</tok> <tok cat="JJ">dominant-negative</tok> <tok cat="NN">suppressor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HLA</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
967982904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="NN">Porcine</tok> <tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NNS">antigens</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">potent</tok> <tok cat="NNS">stimulators</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">direct</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">recognition</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">CD4+</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">are</tok><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">likely</tok> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">rejection</tok> <tok cat="NNS">responses</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">transgenic</tok> <tok cat="NN">pig</tok> <tok cat="NNS">donors</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">clinical</tok> <tok cat="NN">xenotransplantation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
967982905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP" role="PRD"><tok cat="VBD">interested</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">examining</tok> <cons cat="NP"><tok cat="VBN">mutated</tok> <tok cat="NN">CIITA</tok> <tok cat="NNS">constructs</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">porcine</tok> <tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
967982906@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">METHODS</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="JJ">Stable</tok> <tok cat="NNS">transfectants</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">porcine</tok> <tok cat="JJ">vascular</tok> <tok cat="JJ">endothelial</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">PIEC</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="VBN">mutated</tok> <tok cat="NN">CIITA</tok> <tok cat="NNS">constructs</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">tested</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">SLA-DR</tok> <tok cat="CC">and</tok> <tok cat="NN">SLA-DQ</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">recombinant</tok> <tok cat="NN">porcine</tok> <tok cat="NN">interferon-gamma</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
967982907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="JJ">Transient</tok> <tok cat="NNS">transfectants</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">porcine</tok> <tok cat="NN">B-cell</tok> <tok cat="NN">line</tok> <tok cat="NN">L23</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">mutated</tok> <tok cat="NN">CIITA</tok> <tok cat="NNS">constructs</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">tested</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">suppression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">constitutive</tok> <tok cat="NN">SLA-DR</tok> <tok cat="CC">and</tok> <tok cat="NN">SLA-DQ</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
967982908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">T-cell</tok> <tok cat="NN">proliferation</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">performed</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">highly</tok> <tok cat="VBN">purified</tok></cons> <tok cat="JJ">human</tok> <tok cat="JJ">CD4+</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
967982909@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">RESULTS</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="PP"><tok cat="FW">In</tok> <cons cat="NP"><tok cat="JJ">preliminary</tok> <tok cat="NNS">studies</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="NN">transfection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">PIEC</tok> <tok cat="NN">line</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">full-length</tok> <tok cat="JJ">human</tok> <tok cat="NN">CIITA</tok> <tok cat="NNS">constructs</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">strong</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SLA-DR</tok> <tok cat="CC">and</tok> <tok cat="NN">SLA-DQ</tok> <tok cat="NNS">antigens</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">establishing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cross-species</tok> <tok cat="NN">effectiveness</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">CIITA</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pig</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
967982910@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">The</tok> <tok cat="VBN">mutated</tok> <tok cat="JJ">human</tok> <tok cat="NN">CIITA</tok> <tok cat="NNS">constructs</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">tested</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pig</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
967982911@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">PIEC</tok> <tok cat="NNS">clones</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">stably</tok></cons> <tok cat="VBN">transfected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">constructs</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><cons cat="QP"><tok cat="RB">up</tok> <tok cat="TO">to</tok> <tok cat="CD">99</tok></cons><tok cat="NN">%</tok></cons> <tok cat="NN">suppression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SLA-DR</tok> <tok cat="CC">and</tok> <tok cat="NN">SLA-DQ</tok> <tok cat="NN">antigen</tok> <tok cat="NN">induction</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="JJ">marked</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">suppression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SLA-DRA</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">induction</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
967982912@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">transfection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">porcine</tok> <tok cat="NN">B-cell</tok> <tok cat="NN">line</tok> <tok cat="NN">L23</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><cons cat="QP"><tok cat="RB">up</tok> <tok cat="TO">to</tok> <tok cat="CD">90</tok></cons><tok cat="NN">%</tok></cons> <tok cat="NN">suppression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">constitutive</tok> <tok cat="NN">SLA-DR</tok> <tok cat="CC">and</tok> <tok cat="NN">SLA-DQ</tok> <tok cat="NN">antigen</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="CD">5-8</tok> <tok cat="NNS">days</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
967982913@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="NNS">studies</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="JJ">interferon-gamma-stimulated</tok> <tok cat="NN">PIEC</tok> <tok cat="NNS">clones</tok></cons> <cons cat="VP"><tok cat="VBN">transfected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="VBN">mutated</tok> <tok cat="NN">CIITA</tok> <tok cat="NN">construct</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">failed</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">stimulate</tok> <cons cat="NP"><tok cat="VBN">purified</tok> <tok cat="JJ">human</tok> <tok cat="JJ">CD4+</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
967982914@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">CONCLUSION</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Mutated</tok> <tok cat="JJ">human</tok> <tok cat="NN">CIITA</tok> <tok cat="NNS">constructs</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">potent</tok> <tok cat="NNS">suppressors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">porcine</tok> <tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
968018101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Redox</tok> <tok cat="NNS">signals</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
968018102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Accumulating</tok> <tok cat="NNS">data</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">laboratories</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP"><tok cat="VBN">indicated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">NF-kappaB</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">alterations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">redox</tok> <tok cat="NN">homeostasis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">modulating</tok> <cons cat="NP"><tok cat="JJ">immune</tok> <tok cat="NN">function</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
968018103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">recognized</tok> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">genes</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">necessary</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">responses</tok></cons> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-6</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-8</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
968018104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="JJ">Diminished</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">occur</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">aging</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">impaired</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">occur</tok> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NN">senescence</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
968018105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NNS">aberrancies</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">immunopathogenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">diseases</tok></cons> <cons cat="VP"><tok cat="VBG">involving</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">immune</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">inflammatory</tok></cons></cons> <tok cat="NNS">processes</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">atherosclerosis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">infection</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
968018106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">H2O2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">other</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">reactive</tok> <tok cat="NN">oxygen</tok> <tok cat="NNS">species</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ROS</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">integratory</tok> <tok cat="JJ">secondary</tok> <tok cat="NN">messenger</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">divergent</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">signals</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">complicated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">fact</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">various</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">differ</tok> <cons cat="ADVP"><tok cat="RB">markedly</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">oxidant</tok> <tok cat="NN">stress</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@16-12-2010
968018107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Additionally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="JJ">proposed</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">based</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">indirect</tok> <tok cat="NN">evidence</tok></cons> <cons cat="VP"><tok cat="VBN">provided</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">experiments</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">used</tok> <cons cat="NP" id="i9"><tok cat="NNS">antioxidants</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><tok cat="JJ">active</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">formation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
968018108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Further</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">complete</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok> <tok cat="CD">two</tok></cons> <tok cat="NNS">signals</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="IN">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">calcium</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i11"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">enzymatic</tok> <tok cat="NNS">processes</tok></cons> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><tok cat="NNS">kinases</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
968018109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Similarly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="JJ">substantial</tok> <tok cat="NN">evidence</tok></cons> <cons cat="VP"><tok cat="VBZ">indicates</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">full</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP"><tok cat="JJ">dual</tok> <tok cat="NNS">signals</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
968018110@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">ability</tok> <cons cat="S" ref="i13" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">H2O2</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NN">ROS</tok></cons></cons></cons> <cons cat="S" id="i13"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">signals</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="NNS">responses</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="CD">two</tok> <tok cat="NNS">mechanisms</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">reviewed</tok> <cons cat="NP" ref="i12" null="NONE"></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">specific</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">redox</tok> <tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">examined</tok><cons cat="NP" ref="i14" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
968018111@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i15" role="SBJ"><tok cat="NNS">Data</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">presented</tok> <cons cat="S"><cons cat="NP" ref="i15" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">redox</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">relevant</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">immune-related</tok> <tok cat="NNS">diseases</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">aging</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
968661201@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Fibrinogen</tok></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NNS">phagocytes</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
968661202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NN">Adhesion</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">extracellular</tok> <tok cat="NNS">matrices</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">modulate</tok> <cons cat="NP"><tok cat="NN">leukocyte</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <tok cat="RB">not</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">fully</tok></cons> <tok cat="VBN">understood</tok><cons cat="NP" ref="i2" null="NONE"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
968661203@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="JJ">Mononuclear</tok> <tok cat="NNS">phagocytes</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">exposed</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">fibrinous</tok> <tok cat="JJ">provisional</tok> <tok cat="NN">matrix</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">throughout</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">migration</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NNS">foci</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="RB">so</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">undertaken</tok> <cons cat="NP" ref="i4" id="i14" null="NONE"></cons><cons cat="S"><cons cat="NP" ref="i14" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">fibrinogen</tok></cons> <cons cat="VP"><tok cat="VBZ">triggers</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
968661204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">differentiated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">PMA</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">nonadherent</tok> <tok cat="NN">culture</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><cons cat="VP"><tok cat="VB">express</tok> <cons cat="ADVP" id="i12" null="NONE"></cons><cons cat="NP" id="i11"><tok cat="CD">two</tok> <tok cat="JJ">fibrinogen-binding</tok> <tok cat="NNS">integrins</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><cons map="i12" cat="ADVP"><tok cat="RB">predominately</tok></cons> <cons map="i11" cat="NP"><tok cat="NN">CD11b/CD18</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><cons map="i12" cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJR">lesser</tok> <tok cat="NN">extent</tok></cons></cons><tok cat="COMMA">,</tok> <cons map="i11" cat="NP"><tok cat="NN">CD11c/CD18</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
968661205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NNS">Cells</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">fibrinogen</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="QP"><tok cat="CD">10-100</tok></cons> <tok cat="NN">microg/ml</tok></cons><tok cat="RRB">)</tok></cons><tok cat="JJ">/Mn2+</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">50</tok> <tok cat="NN">microM</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="CD">2</tok> <tok cat="NN">h</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
968661206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">activation</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">minimal</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">unstimulated</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">substantially</tok></cons> <tok cat="VBN">up-regulated</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">fibrinogen</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
968661207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Fibrinogen</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">AP-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">SP1</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">cAMP</tok> <tok cat="NN">response</tok> <tok cat="JJ">element-binding</tok> <tok cat="NN">protein</tok> <tok cat="LRB">(</tok><tok cat="NN">CREB</tok><tok cat="RRB">)</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
968661208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" role="SBJ"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="NN">Blocking</tok> <cons cat="NP"><tok cat="NNS">mAbs</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CD18</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CD11b</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">abrogated</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">fibrinogen-induced</tok></cons> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">activation</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
968661209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">regulation</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">transfected</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">plasmid</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">enhancer</tok></cons> <tok cat="LRB">(</tok><cons cat="VP"><tok cat="VBG">bearing</tok> <cons cat="NP"><tok cat="CD">two</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NNS">sites</tok></cons></cons><tok cat="RRB">)</tok> <cons cat="VP"><tok cat="VBN">coupled</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">chloramphenicol</tok> <tok cat="NN">acetyltransferase</tok> <tok cat="LRB">(</tok><tok cat="NN">CAT</tok><tok cat="RRB">)</tok> <tok cat="NN">reporter</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
968661210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="NNS">Cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">subsequently</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" id="i10"><cons cat="LST"><tok cat="LS">1</tok><tok cat="RRB">)</tok></cons> <tok cat="NN">PMA</tok></cons></cons> <cons cat="PP" id="i20" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="CD">24</tok> <tok cat="NN">h</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" id="i21" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><tok cat="VBG">inducing</tok> <cons cat="NP"><tok cat="NN">CAT</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">2.6-fold</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><cons map="i10" cat="NP"><cons cat="LST"><tok cat="LS">2</tok><tok cat="RRB">)</tok></cons> <tok cat="NN">fibrinogen/Mn2+</tok></cons> <cons map="i20" cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="CD">2</tok> <tok cat="NN">h</tok></cons></cons><tok cat="COMMA">,</tok> <cons map="i21" cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><tok cat="VBG">inducing</tok> <cons cat="NP"><tok cat="NN">CAT</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">3.2-fold</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="VP"><cons map="i10" cat="NP"><cons cat="LST"><tok cat="LS">3</tok><tok cat="RRB">)</tok></cons> <cons cat="NP"><tok cat="NN">costimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">fibrinogen</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">PMA</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons map="i20" cat="PP" null="NONE"></cons><cons map="i21" cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inducing</tok> <cons cat="ADVP"><tok cat="JJ">5.7-fold</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CAT</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PMA</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@50@@oe@16-12-2010
968661211@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i13" role="SBJ"><cons cat="NP"><tok cat="NN">contact</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">fibrinogen-derived</tok> <tok cat="NNS">proteins</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NN">phagocyte</tok> <tok cat="NN">activation</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">signaling</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="NN">CD11b/CD18</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i13" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">selective</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">factors</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
968738501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Ciprofloxacin</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">immunomodulatory</tok> <tok cat="NN">stress</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
968738502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Exposure</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">adverse</tok> <tok cat="JJ">environmental</tok> <tok cat="NNS">conditions</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">invokes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">genetically</tok> <tok cat="VBN">programmed</tok></cons> <tok cat="NNS">series</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">events</tok></cons></cons> <cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
968738503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">fluoroquinolone</tok> <tok cat="JJ">antibiotic</tok> <tok cat="NN">ciprofloxacin</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">reported</tok> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">upregulate</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-2</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">gene</tok> <tok cat="NN">induction</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
968738504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">investigation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ciprofloxacin</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="JJ">supratherapeutic</tok> <tok cat="NNS">concentrations</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">immediate-early</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="ADJP"><cons cat="QP"><tok cat="JJR">&lt;</tok><tok cat="CD">2</tok></cons> <tok cat="NN">h</tok></cons><tok cat="RRB">)</tok></cons> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">studied</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">Northern</tok> <tok cat="NNS">blots</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
968738505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="CC">and</tok> <tok cat="NN">metallothionein</tok> <tok cat="NN">enhancer</tok> <tok cat="CC">and</tok> <tok cat="NN">promoter</tok> <tok cat="NNS">regions</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">AP-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">chloramphenicol</tok> <tok cat="NN">acetyltransferase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CAT</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
968738506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">concentration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-fos</tok><tok cat="COMMA">,</tok> <tok cat="NN">c-jun</tok><tok cat="COMMA">,</tok> <tok cat="NN">c-myc</tok><tok cat="COMMA">,</tok> <tok cat="NN">junB</tok><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <tok cat="NN">fra-1</tok> <tok cat="NNS">mRNAs</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">increased</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBN">incubated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">ciprofloxacin</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="VBN">compared</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">untreated</tok> <tok cat="NNS">controls</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
968738507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Ciprofloxacin</tok></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><tok cat="NN">CAT</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">stimulated</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBN">transfected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">plasmids</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="CC">either</tok> <tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">metallothionein</tok> <tok cat="NN">enhancer</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
968738508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">among</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="VP"><tok cat="VBN">tested</tok><cons cat="NP" null="NONE"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="NN">AP-1</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">increased</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">stimulated</tok> <tok cat="VBN">purified</tok> <tok cat="NN">T</tok> <tok cat="NN">helper</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBN">incubated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">ciprofloxacin</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="VBN">compared</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">drug-free</tok> <tok cat="NNS">controls</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
968738509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" id="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">Taken</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">together</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">ciprofloxacin</tok></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">immediate-early</tok> <tok cat="NNS">transcripts</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">enhanced</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="CC">and</tok> <tok cat="NN">metallothionein</tok> <tok cat="NN">promoter</tok> <tok cat="NN">induction</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">upregulated</tok> <cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="NNS">concentrations</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NNS">lymphocytes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">reflecting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">program</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">commonly</tok></cons> <tok cat="VBN">observed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">mammalian</tok> <tok cat="NN">stress</tok> <tok cat="NNS">responses</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
969045501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">Ex</tok> <tok cat="FW">vivo</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">tumor-draining</tok> <tok cat="NN">lymph</tok> <tok cat="NN">node</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">reverses</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBZ">defects</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">transduction</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
969045502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">adoptive</tok> <tok cat="NN">transfer</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">tumor-draining</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lymph</tok> <tok cat="NN">node</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LN</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">activated</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="FW">ex</tok> <tok cat="FW">vivo</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">anti-CD3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="CD">2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-2</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">mediates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">poorly</tok> <tok cat="JJ">immunogenic</tok></cons> <tok cat="JJ">murine</tok> <tok cat="NN">melanoma</tok> <tok cat="NN">D5</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
969045503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">efficacy</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">activated</tok> <tok cat="NN">LN</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">augmented</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i2"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="VBG">sensitizing</tok> <tok cat="NN">tumor</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">genetically</tok> <tok cat="VBN">modified</tok></cons> <tok cat="NN">variant</tok></cons> <tok cat="LRB">(</tok><cons cat="VP"><tok cat="VBN">designated</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NN">D5G6</tok></cons></cons></cons><tok cat="RRB">)</tok> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">secretes</tok> <cons cat="NP"><tok cat="JJ">granulocyte/macrophage-colony-stimulating</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i2" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
969045504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <tok cat="NN">contrast</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">anti-CD3/IL-2-activated</tok> <tok cat="NN">LN</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">adoptive</tok> <tok cat="NN">transfer</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">freshly</tok> <tok cat="VBN">isolated</tok></cons> <tok cat="JJ">tumor-draining</tok> <tok cat="NN">LN</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="JJ">therapeutic</tok> <tok cat="NN">activity</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
969045505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">acquisition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">antitumor</tok> <tok cat="NN">function</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="FW">ex</tok> <tok cat="FW">vivo</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">modifications</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">transduction</tok> <tok cat="NN">capacity</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">protein</tok> <tok cat="NN">tyrosine</tok> <tok cat="NNS">kinases</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p56lck</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p59fyn</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">proteins</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">family</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">tumor-draining</tok> <tok cat="NN">LN</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
969045506@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p56lck</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p59fyn</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="JJR">lower</tok></cons> <cons cat="PP" ref="i6" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">tumor-draining</tok> <cons cat="NP" null="QSTN"></cons></cons></cons><cons cat="PP" id="i6"><tok cat="IN">than</tok> <cons cat="FRAG"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">LN</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">tyrosine-phosphorylated</tok> <tok cat="NNS">substrates</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">markedly</tok></cons> <tok cat="VBN">depressed</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="VBG">following</tok> <cons cat="NP"><tok cat="JJ">anti-CD3</tok> <tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
969045507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">After</tok> <cons cat="NP"><tok cat="JJ">5-day</tok> <tok cat="JJ">anti-CD3/IL-2</tok> <tok cat="NN">activation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p56lck</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p59fyn</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBN">increased</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
969045508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Interestingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p56lck</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p59fyn</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="JJR">higher</tok></cons> <cons cat="SBAR" ref="i9" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">LN</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="IN">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">drained</tok> <cons cat="NP"><tok cat="NN">D5G6</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="SBAR" id="i9"><tok cat="IN">than</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD" null="QSTN"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">D5</tok> <tok cat="NNS">tumors</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
969045509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cytoplasmic</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Rel</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Rel</tok> <tok cat="NN">A</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">normal</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">freshly</tok> <tok cat="VBN">isolated</tok></cons> <tok cat="JJ">tumor-draining</tok> <tok cat="NN">LN</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="SINV"><tok cat="VBD">was</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">kappaB</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">anti-CD3</tok> <tok cat="NN">mAb</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">myristate</tok> <tok cat="NN">acetate</tok></cons></cons></cons></cons></cons><cons cat="ADJP" role="PRD" null="QSTN"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
969045510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">LN</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">D5</tok> <tok cat="NN">tumor</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
969045511@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">recovery</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBG">mediating</tok> <cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">receptor/CD3</tok> <tok cat="NN">complex</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">LN</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">activated</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="FW">ex</tok> <tok cat="FW">vivo</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">acquisition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">antitumor</tok> <tok cat="NN">function</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
969091701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">gamma-IFN</tok> <tok cat="JJ">responsive</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">scavenger</tok> <tok cat="NN">receptor</tok> <tok cat="NN">over-expressing</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">xanthomatosis</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
969091702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <tok cat="VBN">described</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="VBN">inherited</tok> <tok cat="NN">over-expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">macrophage</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">scavenger</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">blood</tok> <tok cat="NNS">monocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">kindred</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i2"><cons cat="NP"><cons cat="QP"><tok cat="RB">only</tok> <tok cat="CD">two</tok></cons></cons> <cons cat="WHPP"><tok cat="IN">of</tok> <cons cat="WHNP"><tok cat="WP">whom</tok></cons></cons></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">displayed</tok> <cons cat="NP"><tok cat="JJ">extensive</tok> <tok cat="NN">xanthomatosis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
969091703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><tok cat="NN">mRNA</tok> <tok cat="JJ">differential</tok> <tok cat="NN">display</tok></cons></cons></cons> <cons cat="NP" id="i3" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">abnormally</tok> <tok cat="JJ">high</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">signal</tok> <tok cat="NN">transducer</tok> <tok cat="CC">and</tok> <tok cat="NN">activator</tok> <tok cat="IN">of</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">STAT1alpha</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">proband</tok> <tok cat="NN">II-2</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
969091704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">gamma-interferon</tok> <tok cat="JJ">inducible</tok> <tok cat="NN">protein</tok> <tok cat="CD">10</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IP-10</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">STAT1alpha-responsive</tok> <tok cat="NN">gene</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">mediator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">abnormally</tok></cons> <tok cat="VBN">expressed</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">monocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">II-2</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
969091705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">Over-expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="JJ">restricted</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">II-2</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">clinically</tok> <tok cat="JJ">unaffected</tok></cons> <tok cat="NN">family</tok> <tok cat="NNS">members</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">unlike</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SR</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
969091706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Gel</tok> <tok cat="NN">retardation</tok> <tok cat="NNS">assays</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">THP-1</tok> <tok cat="NN">cell</tok> <tok cat="NNS">extracts</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">gamma-IFN</tok> <tok cat="JJ">inducible</tok></cons> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="CD">three</tok> <tok cat="JJ">potential</tok> <tok cat="NN">STATI</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NNS">elements</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">IP-10</tok> <tok cat="NN">promoter</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NNS">nucleotides</tok> <tok cat="CD">- 245</tok> <tok cat="TO">to</tok> <tok cat="CD">- 188</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
969091707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i6" id="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">Taken</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">together</tok></cons></cons></cons> <cons cat="NP" id="i6" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP"><tok cat="NN">gamma-interferon</tok> <tok cat="VBD">mediated</tok></cons> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">responsible</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">STAT1alpha-induced</tok></cons> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IP-10</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">THP-1</tok> <tok cat="NNS">macrophages</tok></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">II-2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
969091708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><cons cat="ADJP"><tok cat="JJ">familial</tok> <tok cat="JJ">hypercholesterolemic</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="ADJP"><tok cat="NN">FH</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">subjects</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WP">who</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="ADVP" adv="TMP"><tok cat="RB">frequently</tok></cons> <cons cat="VP"><tok cat="VBP">develop</tok> <cons cat="NP"><tok cat="NN">xanthomatosis</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">significant</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">subjects</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">elevated</tok> <tok cat="NN">STAT1alpha</tok> <tok cat="CC">and</tok> <tok cat="NN">IP-10</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
969091709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inflammatory</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">gamma-IFN</tok> <tok cat="NN">signaling</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">foam</tok> <tok cat="NN">cell</tok> <tok cat="NN">formation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">xanthomatosis</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
969684401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Recognition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">herpes</tok> <tok cat="NN">simplex</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">2</tok> <tok cat="NN">tegument</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD4</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">infiltrating</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">genital</tok> <tok cat="NN">herpes</tok> <tok cat="NNS">lesions</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
969684402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">local</tok> <tok cat="JJ">cellular</tok> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">herpes</tok> <tok cat="NN">simplex</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HSV</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">recurrent</tok> <tok cat="NN">HSV</tok> <tok cat="NN">infection</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
969684403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">antiviral</tok> <tok cat="NNS">functions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBG">infiltrating</tok> <tok cat="JJ">CD4-bearing</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VBP">include</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">cytotoxicity</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">growth</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">lymphokine</tok> <tok cat="NN">secretion</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">support</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="UCP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">humoral</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CD8</tok></cons></cons> <tok cat="NNS">responses</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
969684404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">antigens</tok></cons> <cons cat="VP"><tok cat="VBN">recognized</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP"><tok cat="JJ">many</tok> <tok cat="JJ">HSV-specific</tok> <tok cat="NN">CD4</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">localizing</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">genital</tok> <tok cat="NN">HSV-2</tok> <tok cat="NNS">lesions</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unknown</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
969684405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">recognizing</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">antigens</tok></cons> <cons cat="VP"><tok cat="VBN">encoded</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">map</tok> <tok cat="NNS">units</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">0.67</tok></cons> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="CD">0.73</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HSV</tok> <tok cat="NN">DNA</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">frequently</tok></cons> <tok cat="VBN">recovered</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">herpetic</tok> <tok cat="NNS">lesions</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
969684406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok> <tok cat="NN">cloning</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DNA</tok></cons></cons></cons></cons></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="VP"><tok cat="VBZ">shows</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">tegument</tok> <tok cat="NN">protein</tok> <tok cat="NN">VP22</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">viral</tok> <tok cat="NN">dUTPase</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">encoded</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NNS">genes</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">UL49</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">UL50</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><tok cat="NN">T-cell</tok> <tok cat="NNS">antigens</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
969684407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="JJ">Separate</tok> <tok cat="NNS">epitopes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">VP22</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">defined</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NNS">clones</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">each</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">three</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
969684408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="NN">Reactivity</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">tegument</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBN">encoded</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">UL21</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">additional</tok> <tok cat="NN">patient</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
969684409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="CD">Three</tok> <tok cat="JJ">new</tok> <tok cat="NNS">epitopes</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">VP16</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">tegument</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">VP22</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
969684410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">Some</tok> <tok cat="JJ">tegument-specific</tok> <tok cat="NN">CD4</tok> <tok cat="NN">T-cell</tok> <tok cat="NNS">clones</tok></cons> <cons cat="VP"><tok cat="VBD">exhibited</tok> <cons cat="NP"><tok cat="JJ">cytotoxic</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="JJ">HSV-infected</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
969684411@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">herpes</tok> <tok cat="NN">simplex</tok> <tok cat="NN">tegument</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">processed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">antigen</tok> <tok cat="NN">presentation</tok></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">possible</tok> <tok cat="NN">candidate</tok> <tok cat="NNS">compounds</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">herpes</tok> <tok cat="NN">simplex</tok> <tok cat="NNS">vaccines</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
969784401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Targeted</tok> <tok cat="NN">disruption</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">MyD88</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">loss</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">IL-1-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">IL-18-mediated</tok></cons></cons> <tok cat="NN">function</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
969784402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NN">MyD88</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">originally</tok></cons> <tok cat="VBN">isolated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">differentiation</tok> <tok cat="JJ">primary</tok> <tok cat="NN">response</tok> <tok cat="NN">gene</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i1" id="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">act</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">adaptor</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">signaling</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">interacting</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="CC">both</tok> <tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="NN">receptor</tok> <tok cat="NN">complex</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="JJ">receptor-associated</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IRAK</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
969784403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Mice</tok></cons> <cons cat="VP"><tok cat="VBN">generated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gene</tok></cons> <cons cat="VP"><tok cat="NN">targeting</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">lack</tok> <cons cat="NP"><tok cat="NN">MyD88</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><tok cat="VBZ">defects</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">proliferation</tok></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="NN">phase</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">proteins</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IL-1</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
969784404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="NNS">Increases</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">interferon-gamma</tok> <tok cat="NN">production</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">natural</tok> <tok cat="NN">killer</tok> <tok cat="NN">cell</tok> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IL-18</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">abrogated</tok><cons cat="NP" ref="i3" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
969784405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="ADJP"><tok cat="FW">In</tok> <tok cat="FW">vivo</tok></cons> <tok cat="NN">Th1</tok> <tok cat="NN">response</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="JJ">impaired</tok><cons cat="NP" ref="i4" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@16-12-2010
969784406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">IL-18-induced</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-Jun</tok> <tok cat="JJ">N-terminal</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">JNK</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">MyD88-/-</tok> <tok cat="NN">Th1-developing</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
969784407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i6" id="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">Taken</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">together</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">MyD88</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">critical</tok> <tok cat="NN">component</tok></cons> <cons cat="SBAR" ref="i8" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">signaling</tok> <tok cat="NN">cascade</tok></cons></cons> <cons cat="SBAR" id="i8"><cons cat="WHNP" id="i9"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" id="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="NN">receptor</tok></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><tok cat="NN">IL-18</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
970005301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Human</tok> <tok cat="NN">15-lipoxygenase</tok> <tok cat="NN">gene</tok> <tok cat="NN">promoter</tok></cons><tok cat="COLON">:</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">analysis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">identification</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">IL-13-induced</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">factors</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
970005302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">In</tok> <tok cat="NN">order</tok> <tok cat="TO">to</tok> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VB">study</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">15-LO</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i1" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">cloned</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">sequenced</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons></cons><cons cat="NP" id="i2"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">15-LO</tok> <tok cat="NN">promoter</tok> <tok cat="NN">region</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
970005303@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">15-LO</tok> <tok cat="NN">promoter</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">CpG</tok> <tok cat="NN">island</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">5'-end</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">sequence</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBZ">reveals</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">putative</tok> <tok cat="NN">Sp1</tok> <tok cat="CC">and</tok> <tok cat="NN">Ap2</tok> <tok cat="NN">binding</tok> <tok cat="NNS">site/s</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">absence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TATA</tok> <tok cat="CC">or</tok> <tok cat="NN">CAAT</tok> <tok cat="NNS">motifs</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
970005304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="NN">Transcription</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">initiated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="CD">one</tok> <tok cat="JJ">major</tok> <tok cat="NN">site</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@16-12-2010
970005305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><tok cat="NN">deletion</tok> <tok cat="NNS">constructs</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">defined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">active</tok> <tok cat="NN">promoter</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">1056</tok></cons> <cons cat="NP"><tok cat="NN">bp</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
970005306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Gel-shift</tok> <tok cat="NNS">assays</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">factor</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-13</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">15-LO</tok> <tok cat="NN">promoter</tok> <tok cat="NN">DNA</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
970005307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">Two</tok> <tok cat="NNS">regions</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">DP1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">-140</tok> <tok cat="TO">to</tok> <tok cat="CD">-92</tok> <tok cat="NN">bp</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">DP2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">-353</tok> <tok cat="TO">to</tok> <tok cat="CD">-304</tok> <tok cat="NN">bp</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HeLa</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
970005308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Hela</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">specific</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons> <cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">15-LO</tok> <tok cat="NN">promoter</tok> <tok cat="NN">DNA</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">distinct</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">IL-13-treated</tok> <tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">monocyte</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
970005309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">fluorescent</tok> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">situ</tok></cons> <tok cat="NN">hybridization</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">FISH</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="VBZ">results</tok></cons> <cons cat="VP"><tok cat="VBD">refined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">previous</tok> <tok cat="NN">localization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">15-LO</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">chromosome</tok> <tok cat="NN">17p13.3</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
970010101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">Changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">PKC</tok> <tok cat="NNS">isoforms</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">alveolar</tok> <tok cat="NNS">macrophages</tok></cons></cons> <cons cat="PP"><tok cat="VBN">compared</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">blood</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
970010102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Alveolar</tok> <tok cat="NNS">macrophages</tok></cons> <cons cat="VP"><tok cat="VBP">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">host</tok> <tok cat="NN">defense</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">types</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NNS">processes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">lung</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
970010103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">exhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">many</tok> <tok cat="NNS">alterations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">function</tok></cons></cons></cons> <cons cat="PP"><tok cat="VBN">compared</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">precursor</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">blood</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
970010104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">evaluate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potential</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">differences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">function</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i1" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">evaluated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok> <tok cat="LRB">(</tok><tok cat="NN">PKC</tok><tok cat="RRB">)</tok> <tok cat="NNS">isoforms</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
970010105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Ca2+-dependent</tok> <tok cat="NN">PKC</tok> <tok cat="NNS">isoforms</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="VBN">compared</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">alveolar</tok> <tok cat="NNS">macrophages</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
970010106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">differential</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">Ca2+-independent</tok> <tok cat="NNS">isoforms</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">alveolar</tok> <tok cat="NNS">macrophages</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="VBN">compared</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
970010107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">One</tok> <tok cat="NN">consequence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PKC</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">mitogen-activated</tok> <tok cat="NN">protein</tok> <tok cat="LRB">(</tok><tok cat="NN">MAP</tok><tok cat="RRB">)</tok> <tok cat="NN">kinase</tok> <tok cat="NNS">pathways</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
970010108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">evaluated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">MAP</tok> <tok cat="NN">kinase</tok> <tok cat="JJ">extracellular</tok> <tok cat="JJ">signal-regulated</tok> <tok cat="NN">kinase</tok> <tok cat="LRB">(</tok><tok cat="NN">ERK</tok><tok cat="RRB">)</tok> <tok cat="CD">2</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phorbol</tok> <tok cat="NN">ester</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">12-myristate</tok> <tok cat="NN">13-acetate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PMA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
970010109@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PMA</tok></cons> <cons cat="VP"><tok cat="JJ">activated</tok> <cons cat="NP"><tok cat="NN">ERK2</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="JJ">alveolar</tok> <tok cat="NNS">macrophages</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <tok cat="RB">however</tok><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">monocytes</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">consistently</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">significantly</tok> <tok cat="JJR">greater</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ERK2</tok> <tok cat="NN">kinase</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">PMA</tok></cons></cons> <cons cat="PP"><tok cat="VBN">compared</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">alveolar</tok> <tok cat="NNS">macrophages</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
970010110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">Another</tok> <tok cat="JJ">known</tok> <tok cat="NN">consequence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PKC</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">subsequent</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ERK</tok> <tok cat="NN">kinase</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">activator</tok> <tok cat="NN">protein-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AP-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
970010111@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">evaluated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">PMA</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
970010112@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">very</tok> <tok cat="JJ">little</tok></cons> <tok cat="JJ">detectable</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">assessed</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">gel</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">alveolar</tok> <tok cat="NNS">macrophages</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">monocytes</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">substantial</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">PMA</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
970010113@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">differential</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PKC</tok> <tok cat="NNS">isoforms</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">alveolar</tok> <tok cat="NNS">macrophages</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">blood</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">important</tok> <tok cat="JJ">functional</tok> <tok cat="NNS">alterations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
970756501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Acetylation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">modulation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="JJ">Kruppel-like</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EKLF</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">histone</tok> <tok cat="NNS">acetyltransferases</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
970756502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Erythroid</tok> <tok cat="JJ">Kruppel-like</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EKLF</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">red</tok> <tok cat="JJ">cell-specific</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activator</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">crucial</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">consolidating</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">switch</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="NN">beta-globin</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NN">ontogeny</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
970756503@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="NN">EKLF</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">integrity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">chromatin</tok> <tok cat="NN">structure</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">beta-like</tok> <tok cat="NN">globin</tok> <tok cat="NN">locus</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">interacts</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">positive-acting</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
970756504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">find</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">EKLF</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">an</tok> <tok cat="VBN">acetylated</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">interacts</tok> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CBP</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p300</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">P/CAF</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
970756505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NNS">interactions</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NN">histone</tok> <tok cat="NNS">acetyltransferases</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">equivalent</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">CBP</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p300</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">P/CAF</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">utilize</tok> <cons cat="NP"><tok cat="NN">EKLF</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">substrate</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">acetylation</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">trans-activation</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
970756506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">functional</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">interactions</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="SBAR" role="PRD"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">CBP</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p300</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">P/CAF</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">enhance</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">EKLF</tok><tok cat="POS">'s</tok></cons> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">beta-globin</tok> <tok cat="NN">promoter</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
970756507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">establish</tok> <cons cat="NP"><tok cat="NN">EKLF</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">tissue-specific</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">undergoes</tok> <cons cat="NP"><tok cat="JJ">post-translational</tok> <tok cat="NN">acetylation</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i5"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">EKLF</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">alter</tok> <cons cat="NP"><tok cat="NN">chromatin</tok> <tok cat="NN">structure</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><tok cat="NN">beta-globin</tok> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">beta-like</tok> <tok cat="NN">globin</tok> <tok cat="NN">cluster</tok></cons></cons><cons cat="PP" ref="i5" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
970760801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Epstein-Barr</tok> <tok cat="JJ">virus-transforming</tok> <tok cat="NN">protein</tok> <tok cat="JJ">latent</tok> <tok cat="NN">infection</tok> <tok cat="NN">membrane</tok> <tok cat="NN">protein</tok> <tok cat="CD">1</tok></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">NF-kappaB</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">pathway</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">includes</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappaB-inducing</tok> <tok cat="NN">kinase</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IkappaB</tok> <tok cat="NNS">kinases</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IKKalpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IKKbeta</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
970760802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok> <tok cat="NN">oncoprotein</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">latent</tok> <tok cat="NN">infection</tok> <tok cat="NN">membrane</tok> <tok cat="NN">protein</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LMP1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">constitutively</tok> <tok cat="VBN">aggregated</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">pseudo-tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNFR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">NF-kappaB</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">C-terminal</tok> <tok cat="JJ">cytoplasmic</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
970760803@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="CD">One</tok> <tok cat="NN">site</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">similar</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">TNFRII</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="PP"><tok cat="VBG">associating</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">TNFR-associated</tok> <tok cat="NNS">factors</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">TRAF1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TRAF2</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">second</tok> <tok cat="NN">site</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">similar</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">TNFRI</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="PP"><tok cat="VBG">associating</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">TNFRI</tok> <tok cat="NN">death</tok> <tok cat="NN">domain</tok> <tok cat="VBG">interacting</tok> <tok cat="NN">protein</tok> <tok cat="NN">TRADD</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
970760804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">TNFRI</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">association</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">TRADD</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">RIP</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TRAF2</tok></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappaB-inducing</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NIK</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IkappaB</tok> <tok cat="NN">alpha</tok> <tok cat="NNS">kinases</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IKKalpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IKKbeta</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COLON">;</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IkappaB</tok> <tok cat="NN">alpha</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
970760805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">IkappaB</tok> <tok cat="NN">alpha</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Ser-32</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Ser-36</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">followed</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">degradation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
970760806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">report</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">LMP1</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">each</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">effector</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">pathway</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">includes</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NIK</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IKKalpha</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IKKbeta</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
970760807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Dominant</tok> <tok cat="JJ">negative</tok> <tok cat="NNS">mutants</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NIK</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IKKalpha</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">IKKbeta</tok></cons></cons></cons></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">substantially</tok></cons> <tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">LMP1</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">each</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">effector</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
971014901@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Dimethyldithiocarbamate</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">human</tok> <tok cat="JJ">CD4+</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
971014902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NNS">Dithiocarbamates</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">DTC</tok></cons><tok cat="RRB">)</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">diverse</tok> <tok cat="NN">group</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">industrial</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">therapeutic</tok></cons></cons> <tok cat="NNS">chemicals</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">reported</tok> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">inhibit</tok><cons cat="NP" ref="i2" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBP">enhance</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons></cons><cons cat="NP" id="i2"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immune</tok> <tok cat="NN">system</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
971014903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="JJ">apparent</tok> <tok cat="NNS">inconsistencies</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">reflect</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">complexity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">DTCs</tok> <tok cat="JJ">biological</tok> <tok cat="NNS">activities</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">probably</tok></cons> <cons cat="PP" role="PRD"><tok cat="JJ">due</tok> <cons cat="PRN"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">part</tok></cons></cons></cons> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">differences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">dose</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">route</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">exposure</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">animal</tok> <tok cat="NNS">species</tok></cons> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" null="NONE"></cons></cons></cons><tok cat="CC">and/or</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">compound</tok></cons> <cons cat="VP"><tok cat="VBN">tested</tok><cons cat="NP" null="NONE"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
971014904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBN">described</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">herein</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">undertaken</tok> <cons cat="NP" ref="i3" id="i11" null="NONE"></cons><cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">investigate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">immunotoxicity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok> <tok cat="NN">member</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">family</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">dimethyldithiocarbamate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">DMDTC</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
971014905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP"><cons cat="QP"><tok cat="CD">0.1-0.5</tok></cons> <tok cat="NN">microM</tok></cons> <tok cat="NN">DMDTC</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">TNF-alpha-induced</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">human</tok> <tok cat="JJ">CD4+</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
971014906@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">accompanied</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">loss</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">viability</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">DMDTC-treated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">retain</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="JJ">active</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">throughout</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">exposure</tok> <tok cat="NN">duration</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
971014907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">apparently</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">permanent</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">DMDTC-treated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">regain</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">TNF-alpha</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP" adv="TMP"><cons cat="ADVP"><tok cat="RB">even</tok></cons> <tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">72</tok> <tok cat="NN">h</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">culture</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
971014908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">DMDTC</tok></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">appear</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">alter</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons> <cons cat="ADVP"><tok cat="RB">directly</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">pre-incubation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">DMDTC</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">diminish</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
971014909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">further</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP"><cons cat="QP"><tok cat="CD">0.1-0.5</tok></cons> <tok cat="NN">microM</tok></cons> <tok cat="NN">DMDTC</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">production</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">decreases</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">surface</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD25</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">alpha</tok> <tok cat="NN">subunit</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">receptor</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">ester</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
971014910@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">DMDTC</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <tok cat="JJ">potent</tok> <tok cat="JJ">immunosuppressive</tok> <tok cat="NN">compound</tok></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
971021501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">element</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CD95</tok> <tok cat="LRB">(</tok><tok cat="NN">APO-1/Fas</tok><tok cat="RRB">)</tok> <tok cat="NN">ligand</tok> <tok cat="NN">promoter</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">responsiveness</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">TCR-mediated</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
971021502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CD95</tok> <tok cat="LRB">(</tok><tok cat="NN">APO-1/Fas</tok><tok cat="RRB">)</tok> <tok cat="NN">ligand</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CD95L</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">major</tok> <tok cat="NN">cause</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="JJ">activation-induced</tok></cons> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
971021503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">study</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD95L</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i1" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">CD95L</tok> <tok cat="NN">promoter</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
971021504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Deletion</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CD95L</tok> <tok cat="JJ">proximal</tok> <tok cat="NN">promoter</tok> <tok cat="NN">sequence</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="CD">-220</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">start</tok> <tok cat="NN">site</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="JJ">stimulation-induced</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD95L</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
971021505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">discovered</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">element</tok></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">-120</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CD95L</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">SP-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">yet</tok> <tok cat="JJ">unknown</tok></cons> <tok cat="JJ">inducible</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
971021506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Mutation</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inducible</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">-120</tok> <tok cat="NN">region</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">crucial</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">biological</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CD95L</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">upon</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
971021507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">DNA</tok> <tok cat="NN">sequence</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="CD">-120</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NN">DNA</tok> <tok cat="NNS">motifs</tok></cons> <cons cat="ADJP"><tok cat="JJ">homologous</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBG">binding</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
971021508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NF-AT</tok></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="ADVP"><tok cat="RB">directly</tok></cons> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">element</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
971021509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">cotransfection</tok> <tok cat="NNS">studies</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">NF-AT</tok> <tok cat="NN">expression</tok> <tok cat="NN">vector</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NF-AT</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">confer</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">strong</tok> <tok cat="JJ">inducible</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CD95L</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
971021510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">data</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">immunosuppressive</tok> <tok cat="NN">agent</tok> <tok cat="NN">cyclosporin</tok> <tok cat="NN">A</tok></cons> <cons cat="VP"><tok cat="VBZ">down-regulates</tok> <cons cat="NP"><tok cat="NN">CD95L</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inhibiting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">positive</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
971044801@GENIA Treebank@formal@@1@S@<cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">Highly</tok> <tok cat="VBN">polarized</tok></cons> <tok cat="NN">HLA</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">antigen</tok> <tok cat="NN">processing</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">presentation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">intestinal</tok> <tok cat="JJ">epithelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
971044802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">high</tok> <tok cat="NN">concentration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">foreign</tok> <tok cat="NN">antigen</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">lumen</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">gastrointestinal</tok> <tok cat="NN">tract</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">separated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBG">underlying</tok> <tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">single</tok> <tok cat="NN">cell</tok> <tok cat="NN">layer</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">polarized</tok> <tok cat="NN">epithelium</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
971044803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Intestinal</tok> <tok cat="JJ">epithelial</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">express</tok> <cons cat="NP"><tok cat="NN">HLA</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NNS">antigens</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">function</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">antigen-presenting</tok> <tok cat="NNS">cells</tok> <tok cat="TO">to</tok> <tok cat="JJ">CD4(+)</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">intestinal</tok> <tok cat="NN">mucosa</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
971044804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">tetanus</tok> <tok cat="NN">toxoid</tok> <tok cat="JJ">specific</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">HLA-DR-restricted</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="VBN">polarized</tok> <tok cat="JJ">intestinal</tok> <tok cat="JJ">epithelial</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">directed</tok> <cons cat="NP" null="NONE"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">express</tok> <cons cat="NP"><tok cat="NN">HLA-DR</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="JJ">able</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">initiate</tok> <cons cat="NP"><tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">processing</tok></cons> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <cons cat="PP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">internalization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">antigen</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">apical</tok> <tok cat="NN">surface</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
971044805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Coexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">transactivator</tok> <tok cat="NN">CIITA</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><tok cat="RB">highly</tok> <tok cat="JJ">efficient</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">processing</tok></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">characteristic</tok> <tok cat="NN">decline</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">barrier</tok> <tok cat="NN">function</tok></cons> <cons cat="VP"><tok cat="VBN">seen</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">polarized</tok> <tok cat="NNS">monolayers</tok></cons> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">proinflammatory</tok> <tok cat="NN">cytokine</tok> <tok cat="NN">gamma-IFN</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">facilitates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">antigen</tok> <tok cat="NN">processing</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">basolateral</tok> <tok cat="NN">surface</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
971044806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">cases</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="NN">peptide</tok> <tok cat="NN">presentation</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NNS">molecules</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="JJ">restricted</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">basolateral</tok> <tok cat="NN">surface</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
971044807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="RB">highly</tok> <tok cat="VBN">polarized</tok> <tok cat="JJ">functional</tok> <tok cat="NN">architecture</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">antigen</tok> <tok cat="NN">processing</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">presentation</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">intestinal</tok> <tok cat="JJ">epithelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">functional</tok> <tok cat="NN">outcome</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">antigen</tok> <tok cat="NN">processing</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">intestinal</tok> <tok cat="NN">epithelium</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="UCP" role="PRD"><tok cat="CC">both</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="JJ">dependent</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">surface</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i5"><tok cat="IN">at</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">foreign</tok> <tok cat="NN">antigen</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">internalized</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP" ref="i5" null="T"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="PP" role="PRD"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">underlying</tok> <tok cat="NN">degree</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">mucosal</tok> <tok cat="NN">inflammation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@54@@oe@16-12-2010
971058201@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">small</tok> <tok cat="JJ">GTP-binding</tok> <tok cat="NN">protein</tok> <tok cat="NN">Rho</tok></cons> <cons cat="VP"><tok cat="VBZ">potentiates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
971058202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">Rho</tok> <tok cat="NN">family</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">small</tok> <tok cat="JJ">GTP-binding</tok> <tok cat="NNS">proteins</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">cytoskeletal</tok> <tok cat="NN">structure</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">cell</tok> <tok cat="NN">fate</tok> <tok cat="NN">development</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">transformation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
971058203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">report</tok></cons></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">overexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">activated</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Rho</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">enhances</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">myristate</tok> <tok cat="NN">acetate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PMA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">Rho</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">V14Rho</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">little</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="DT">no</tok></cons></cons> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NFAT</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Oct-1</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">enhancer</tok> <tok cat="NN">element</tok></cons></cons> <tok cat="NNS">activities</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><tok cat="JJ">similar</tok> <tok cat="NNS">conditions</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@56@@oe@16-12-2010
971058204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Overexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">V14Rho</tok> <tok cat="NN">construct</tok></cons> <cons cat="ADJP"><tok cat="JJ">incapable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">membrane</tok> <tok cat="NN">localization</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">CAAX</tok></cons> <cons cat="VP"><tok cat="VBN">deleted</tok><cons cat="NP" null="NONE"></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">abolishes</tok> <cons cat="NP"><tok cat="JJ">PMA-induced</tok> <tok cat="NN">AP-1</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
971058205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Rho</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">independent</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">mitogen-activated</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">pathway</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">dominant-negative</tok> <tok cat="NN">MEK</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">MEK</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PD98059</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><tok cat="JJ">Rho-induced</tok> <tok cat="NN">AP-1</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
971058206@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">V14Rho</tok></cons> <cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">Calpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PKCalpha</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">deletion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CAAX</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">V14Rho</tok></cons></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">severely</tok></cons> <cons cat="VP"><tok cat="VBD">diminished</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">association</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
971058207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="NN">Evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">PKCalpha</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">effector</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Rho</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">observation</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">coexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">N-terminal</tok> <tok cat="NN">domain</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PKCalpha</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">blocked</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">Rho</tok></cons> <tok cat="CC">plus</tok> <cons cat="NP"><tok cat="NN">PMA</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
971058208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Rho</tok></cons> <cons cat="VP"><tok cat="VBZ">potentiates</tok> <cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
971060001@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok> <tok cat="NN">Tax</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IkappaB</tok> <tok cat="NN">kinase</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IKKalpha</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IKKbeta</tok></cons> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NNS">kinases</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
971060002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Tax</tok></cons> <cons cat="VP"><tok cat="VBZ">corresponds</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">40-kDa</tok> <tok cat="VBG">transforming</tok> <tok cat="NN">protein</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">pathogenic</tok> <tok cat="NN">retrovirus</tok> <tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HTLV-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappaB/Rel</tok> <tok cat="NN">family</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">unknown</tok> <tok cat="NN">mechanism</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
971060003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Tax</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">promotes</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">N-terminal</tok> <tok cat="NN">phosphorylation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">degradation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IkappaB</tok> <tok cat="NN">alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">principal</tok> <cons cat="NP"><tok cat="JJ">cytoplasmic</tok> <tok cat="NN">inhibitor</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
971060004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">studies</tok></cons> <cons cat="ADVP"><tok cat="RB">now</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">HTLV-1</tok></cons> <cons cat="NP"><tok cat="NN">Tax</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">recently</tok> <tok cat="VBN">identified</tok></cons> <tok cat="JJ">cellular</tok> <tok cat="NNS">kinases</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">IkappaB</tok> <tok cat="NN">kinase</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IKKalpha</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IKKbeta</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">normally</tok></cons> <cons cat="VP"><tok cat="VBP">phosphorylate</tok> <cons cat="NP"><tok cat="NN">IkappaB</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">both</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">N-terminal</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">serines</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@16-12-2010
971060005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">mutant</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Tax</tok></cons> <cons cat="VP"><tok cat="VBN">termed</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NN">M22</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">fails</tok> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="NN">IKKalpha</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">IKKbeta</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
971060006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="JJ">endogenous</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">IKK</tok> <tok cat="JJ">enzymatic</tok></cons> <tok cat="NN">activity</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP"><tok cat="JJ">elevated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">HTLV-1-infected</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">Tax-expressing</tok></cons></cons> <tok cat="NN">T-cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
971060007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Transfection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">kinase-deficient</tok> <tok cat="NNS">mutants</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IKKalpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IKKbeta</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <cons cat="NP" ref="i11" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="CD">293</tok> <cons cat="NP" ref="i11" null="RNR"></cons></cons><cons cat="NP" id="i11"><tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">NF-kappaB-dependent</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">Tax</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
971060008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Similarly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">kinase-deficient</tok> <tok cat="NN">mutant</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NIK</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-kappaB-inducing</tok> <tok cat="NN">kinase</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">represents</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">upstream</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <cons cat="NP" ref="i12" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-1</tok> <cons cat="NP" ref="i12" null="RNR"></cons></cons><cons cat="NP" id="i12"><tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons></cons> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IKKalpha</tok> <cons cat="NP" ref="i13" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IKKbeta</tok> <cons cat="NP" ref="i13" null="RNR"></cons></cons><cons cat="NP" id="i13"><tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">blocks</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Tax</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
971060009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="NN">plasma</tok> <tok cat="JJ">membrane-proximal</tok> <tok cat="NNS">elements</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="JJ">proinflammatory</tok> <tok cat="NN">cytokine</tok> <tok cat="NNS">pathways</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">apparently</tok></cons> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="SBAR"><tok cat="IN">since</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">dominant</tok> <cons cat="NP"><tok cat="JJ">negative</tok> <tok cat="NNS">mutants</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">TRAF2</tok> <cons cat="NP" ref="i8" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TRAF6</tok> <cons cat="NP" ref="i8" null="RNR"></cons></cons><cons cat="NP" id="i8"><tok cat="NNS">adaptors</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">effectively</tok></cons> <cons cat="VP"><tok cat="VBP">block</tok> <cons cat="NP"><tok cat="NN">signaling</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cytoplasmic</tok> <tok cat="NNS">tails</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">TNF-alpha</tok> <cons cat="NP" ref="i10" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-1</tok> <cons cat="NP" ref="i10" null="RNR"></cons></cons><cons cat="NP" id="i10"><tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBP">do</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Tax</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@50@@oe@16-12-2010
971060010@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Together</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">HTLV-1</tok></cons> <cons cat="NP"><tok cat="NN">Tax</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">exploits</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">distal</tok> <tok cat="NN">part</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">proinflammatory</tok> <tok cat="NN">cytokine</tok> <tok cat="NN">signaling</tok> <tok cat="NN">cascade</tok></cons> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
971060011@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">pathological</tok> <tok cat="NN">alteration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">cytokine</tok> <tok cat="NN">pathway</tok></cons> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">Tax</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">central</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">HTLV-1-mediated</tok> <tok cat="NN">transformation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">clinically</tok></cons> <cons cat="VP"><tok cat="VBN">manifested</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">adult</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
971201901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">Class</tok> <tok cat="CD">II</tok> <tok cat="JJ">transactivator-independent</tok></cons> <tok cat="JJ">endothelial</tok> <tok cat="NN">cell</tok> <tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">gene</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">lymphocyte</tok> <tok cat="NN">adhesion</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
971201902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NNS">molecules</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">surface</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">allogeneic</tok> <tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">adhesion-dependent</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">IFN-gamma-independent</tok></cons></cons> <tok cat="NN">manner</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
971201903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><tok cat="NN">HLA-DR</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">surface</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">mutant</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">defective</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">IFN-gamma-induced</tok> <tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
971201904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">RNA</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">defective</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">transactivator</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CIITA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">demonstrates</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP"><tok cat="NN">NK</tok> <tok cat="JJ">cell-induced</tok></cons> <tok cat="NN">HLA-DR</tok> <tok cat="NN">alpha</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">CIITA-independent</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
971201905@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">Janus</tok> <tok cat="JJ">kinase-1-deficient</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">U4A</tok></cons> <cons cat="VP"><tok cat="VBZ">expresses</tok> <cons cat="NP"><tok cat="NN">HLA-DR</tok> <tok cat="NN">alpha</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">NK</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="NN">HLA-DR</tok> <tok cat="NN">alpha</tok> <tok cat="NN">promoter</tok> <tok cat="NNS">constructs</tok></cons> <cons cat="VP"><tok cat="VBN">transfected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
971201906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">IFN-gamma-independent</tok> <tok cat="NN">component</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">target</tok> <tok cat="NN">cell</tok> <tok cat="NN">HLA-DR</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">lymphocyte</tok> <tok cat="NN">adhesion</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">uses</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">distinct</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">IFN-gamma-dependent</tok> <tok cat="NN">mechanism</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <tok cat="RB">also</tok> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">CIITA</tok></cons> <cons cat="VP"><tok cat="VBD">is</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">required</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
971202601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">CD28-associated</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok></cons> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">proliferation</tok></cons></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP"><tok cat="NN">TCR</tok> <tok cat="NN">engagement</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
971202602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">resting</tok> <tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">CD28-specific</tok> <tok cat="NN">mAb</tok> <tok cat="NN">BW</tok> <tok cat="CD">828</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">proliferation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">synthesis</tok></cons></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">further</tok> <tok cat="NN">requirement</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">TCR</tok> <tok cat="NN">coengagement</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
971202603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">observation</tok></cons> <cons cat="VP"><tok cat="VBD">prompted</tok> <cons cat="NP"><tok cat="PRP">us</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VB">postulate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">signal</tok> <tok cat="CD">2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">costimulatory</tok> <tok cat="NN">signal</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="ADJP"><tok cat="RB">alone</tok></cons></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">signal</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TCR</tok> <tok cat="NN">signal</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
971202604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">test</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">putative</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD28</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">particular</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i2" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">compared</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">events</tok></cons> <cons cat="VP"><tok cat="VBN">initiated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBG">resting</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="JJ">stimulatory</tok> <tok cat="NN">mAb</tok> <tok cat="NN">BW</tok> <tok cat="CD">828</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">signals</tok></cons> <cons cat="VP"><tok cat="VBN">triggered</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="JJ">nonstimulating</tok> <tok cat="NN">CD28</tok> <tok cat="NN">mAb</tok> <tok cat="CD">9.3</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
971202605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">BW</tok> <tok cat="CD">828</tok></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">Ca2+</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">lead</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">detectable</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">protein</tok> <tok cat="NNS">kinases</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p56(lck)</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Raf-1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
971202606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">pathway</tok></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CD28</tok> <tok cat="NN">response</tok> <tok cat="NN">element</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
971202607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">On</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">other</tok> <tok cat="NN">hand</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">mAb</tok> <tok cat="CD">9.3</tok></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">Ca2+</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">triggered</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p56(lck)</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Raf-1</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="JJ">unable</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
971202608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">contrast</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">differential</tok> <tok cat="NN">signaling</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">BW</tok> <tok cat="CD">828</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">9.3</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBG">resting</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="NNS">mAbs</tok></cons> <cons cat="VP"><tok cat="VBD">exhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">similar</tok> <tok cat="NN">pattern</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">events</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">cells</tok> <tok cat="LRB">(</tok><tok cat="NN">p56(lck)</tok> <tok cat="NN">activation</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">association</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">phosphatidylinositol</tok> <tok cat="NN">3-kinase</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">CD28</tok><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">signaling</tok> <tok cat="NN">capacity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD28</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">changes</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@57@@oe@16-12-2010
971202609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">support</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">view</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="NN">CD28</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">some</tok> <tok cat="NN">effector</tok> <tok cat="NNS">functions</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">capacity</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">particular</tok> <tok cat="NN">pattern</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">events</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
971204701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Cyclosporin</tok> <tok cat="JJ">A-resistant</tok> <tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">okadaic</tok> <tok cat="JJ">acid-sensitive</tok> <tok cat="NN">serine/threonine</tok> <tok cat="NNS">phosphatases</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
971204702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">TCR</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">costimulation</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="NN">accessory</tok> <tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
971204703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="ADVP"><tok cat="RB">recently</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="JJ">okadaic</tok> <tok cat="JJ">acid-sensitive</tok> <tok cat="NN">Ser/Thr</tok> <tok cat="NNS">phosphatases</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cyclosporin</tok> <tok cat="JJ">A-insensitive</tok> <tok cat="NN">pathway</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">selectively</tok></cons> <cons cat="VP"><tok cat="VBZ">transmits</tok> <cons cat="NP"><tok cat="JJ">costimulatory</tok> <tok cat="NNS">signals</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
971204704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">analyzed</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">activities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">phosphatases</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">necessary</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
971204705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="JJ">tumorigenic</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i3" role="SBJ"><tok cat="NN">IL-2</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">level</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">okadaic</tok> <tok cat="NN">acid</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
971204706@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok> <tok cat="JJ">active</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">differentially</tok></cons> <cons cat="VP"><tok cat="VBN">influenced</tok><cons cat="NP" ref="i4" null="NONE"></cons><tok cat="COLON">:</tok> <cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">down-modulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">okadaic</tok> <tok cat="JJ">acid-sensitive</tok> <tok cat="NNS">phosphatases</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">octamer</tok><tok cat="COMMA">,</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">NF</tok> <tok cat="IN">of</tok> <tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">abrogated</tok><cons cat="NP" ref="i5" null="NONE"></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="NNS">proteins</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">even</tok></cons> <cons cat="VP"><tok cat="VBN">enhanced</tok><cons cat="NP" ref="i6" null="NONE"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
971206801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Inducible</tok> <tok cat="JJ">nitric</tok> <tok cat="NN">oxide</tok></cons><tok cat="COLON">:</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">autoregulatory</tok> <tok cat="NN">feedback</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">vascular</tok> <tok cat="NN">inflammation</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
971206802@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="JJ">Inducible</tok> <tok cat="JJ">nitric</tok> <tok cat="NN">oxide</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">iNO</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">vascular</tok> <tok cat="NN">inflammation</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">resident</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">nonresident</tok></cons></cons> <tok cat="JJ">vascular</tok> <tok cat="NN">wall</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inflammatory</tok> <tok cat="NN">process</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">known</tok><cons cat="NP" ref="i2" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
971206803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">iNO</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="S" role="PRD"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">terminate</tok> <cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NNS">processes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
971206804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">find</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">iNO</tok></cons> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="JJ">macrophage-like</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">RAW264.7</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><tok cat="JJ">cytokine-induced</tok> <tok cat="JJ">endothelial</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">separated</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">mixed</tok></cons></cons> <tok cat="NN">endothelial-RAW264.7</tok> <tok cat="NN">coculture</tok> <tok cat="NN">system</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
971206805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i3" role="SBJ"><tok cat="CC">Both</tok> <cons cat="NP"><tok cat="NN">iNO</tok> <tok cat="NN">production</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NN">VCAM-1</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">simultaneously</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">bacterial</tok> <tok cat="NN">LPS</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">murine-specific</tok> <tok cat="NN">IFN-gamma</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
971206806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">iNO</tok> <tok cat="NN">synthase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">iNOS</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">N</tok> <tok cat="NN">omega-monomethyl-L-arginine</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">endothelial-RAW264.7</tok> <tok cat="NNS">cocultures</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">murine-specific</tok> <tok cat="NN">IFN-gamma</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">LPS</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">decreased</tok> <cons cat="NP"><tok cat="NN">iNO</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="CD">86</tok><tok cat="NN">%</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">augmented</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">VCAM-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">iNOS</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">endothelial</tok> <cons cat="NP" ref="i4" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">RAW264.7</tok> <cons cat="NP" ref="i4" null="RNR"></cons></cons><cons cat="NP" id="i4"><tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">monocyte</tok> <tok cat="NN">adhesion</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">endothelial</tok> <tok cat="NN">cell</tok> <tok cat="NN">surface</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@52@@oe@16-12-2010
971206807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Transient</tok> <tok cat="NN">transfection</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NN">VCAM-1</tok> <tok cat="NN">promoter</tok> <tok cat="NNS">constructs</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="JJ">inhibitory</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">iNO</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">VCAM-1</tok></cons> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">mediated</tok><cons cat="NP" ref="i5" null="NONE"></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="IN">in</tok> <tok cat="NN">part</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">inhibitory</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">iNO</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="JJ">cis-acting</tok> <tok cat="NNS">elements</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
971206808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Immunofluorescence</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">Ab</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">RelA</tok> <tok cat="LRB">(</tok><tok cat="NN">p65</tok><tok cat="RRB">)</tok> <tok cat="NN">subunit</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">iNO</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
971206809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">iNO</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">attenuates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">iNOS</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">monocyte</tok> <tok cat="NN">adhesion</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP"><tok cat="RB">endogenously</tok> <tok cat="VBN">derived</tok></cons> <tok cat="NN">iNO</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="JJ">autoregulatory</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">vascular</tok> <tok cat="NN">inflammation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
971270301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">peroxisome</tok> <tok cat="JJ">proliferator-activated</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PPARalpha</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">ligand</tok></cons> <cons cat="NP"><tok cat="NN">WY</tok> <tok cat="CD">14,643</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">interfere</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">leukotriene</tok> <tok cat="NN">B4</tok> <tok cat="JJ">induced</tok> <tok cat="NN">adhesion</tok> <cons cat="PP"><tok cat="IN">of</tok> <tok cat="NNS">neutrophils</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
971270302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Peroxisome</tok> <tok cat="JJ">proliferator-activated</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">PPAR</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBP">control</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">discrete</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">fatty</tok> <tok cat="NN">acid</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">lipid</tok> <tok cat="NN">metabolism</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
971270303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Recently</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i1" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">suggested</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">alpha</tok> <tok cat="NN">isoform</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">PPAR</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">potent</tok> <tok cat="JJ">proinflammatory</tok> <tok cat="NN">mediator</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">leukotriene</tok> <tok cat="NN">B4</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LTB4</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">enhanced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">degradation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">eicosanoid</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">offersuggesting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">new</tok> <tok cat="NN">aspect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">down-regulation</tok> <tok cat="IN">of</tok> <tok cat="NN">inflammation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
971270304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">studied</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">PPARalpha</tok> <tok cat="NN">activation</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">means</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">selective</tok> <tok cat="NN">agonist</tok></cons> <cons cat="NP"><tok cat="NN">WY</tok> <tok cat="CD">14,643</tok></cons></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">pivotal</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NNS">responses</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">interfered</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">LTB4</tok> <tok cat="VBD">induced</tok> <tok cat="NN">adhesion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PMN</tok> <tok cat="NN">neutrophil</tok> <tok cat="NNS">granulocytes</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
971270305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" adv="TMP"><tok cat="WRB">When</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">WY</tok> <tok cat="CD">14,643</tok> <cons cat="ADJP"><tok cat="RB">prior</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">LTB4</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">fMLP</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-1beta</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">TNFalpha</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="NNS">controls</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">document</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">any</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">adhering</tok> <tok cat="NN">PMN</tok></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">duration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
971270306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBZ">provides</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">evidence</tok></cons> <cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PPARalpha</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">LTB4</tok> <tok cat="JJ">induced</tok> <tok cat="JJ">adhesive</tok> <tok cat="NNS">interactions</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">neutrophils</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
971272101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">B</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><cons cat="NP" id="i1"><tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">transcription</tok> <cons cat="NP" ref="i9" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">differentiation</tok> <cons cat="NP" ref="i9" null="RNR"></cons></cons><cons cat="NP" id="i9"><tok cat="NNS">factors</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
971272102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">During</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">B</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><cons cat="NP" id="i2"><tok cat="NN">cell</tok> <tok cat="NN">differentiation</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">proliferation</tok></cons></cons></cons> <cons cat="NP" id="i3" role="SBJ"><tok cat="JJ">many</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i3" null="NONE"></cons></cons><tok cat="CC">or</tok> <cons cat="VP"><tok cat="VBN">repressed</tok> <cons cat="NP" ref="i3" null="NONE"></cons></cons><cons cat="SBAR"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="JJ">certain</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok><cons cat="NP" ref="i4" null="NONE"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
971272103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">investigate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">processes</tok></cons> <cons cat="VP"><tok cat="JJ">related</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">B</tok> <cons cat="NP" ref="i5" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <cons cat="NP" ref="i5" null="RNR"></cons></cons><cons cat="NP" id="i5"><tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">stimulated</tok> <cons cat="NP" ref="i6" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">nonstimulated</tok> <cons cat="NP" ref="i6" null="RNR"></cons></cons><cons cat="NP" syn="COOD" id="i6"><cons cat="NP"><tok cat="NN">Ramos</tok> <cons cat="NP" ref="i7" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <cons cat="NP" ref="i7" null="RNR"></cons></cons><cons cat="NP" id="i7"><tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">studied</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="NN">cDNA</tok> <tok cat="NN">microarray</tok> <tok cat="NN">technology</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
971272104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Simultaneous</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="QP"><tok cat="JJ">close</tok> <tok cat="TO">to</tok> <tok cat="CD">600</tok></cons> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">indicated</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJS">highest</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">certain</tok> <cons cat="NP"><tok cat="NN">transcription</tok><cons cat="NP" ref="i10" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">differentiation</tok> <cons cat="NP" ref="i10" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">proliferation</tok> <cons cat="NP" ref="i10" null="RNR"></cons></cons><cons cat="NP" id="i10"><tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
971272105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="JJ">Many</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <tok cat="RB">not</tok> <cons cat="ADVP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="VB">funcion</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">stimulated</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
971272106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Also</tok></cons> <cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="NNS">genes</tok> <tok cat="VBG">encoding</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">replication</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">binding</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">translation</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">induced</tok><cons cat="NP" ref="i12" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
971272107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i13" role="SBJ"><cons cat="NP"><tok cat="JJ">Large</tok> <tok cat="NN">part</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">activated</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">very</tok> <tok cat="JJ">stringent</tok></cons> <tok cat="NN">regulation</tok></cons></cons> <cons cat="VP"><tok cat="VBG">being</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <cons cat="PP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
971272108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i14" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP" ref="i15" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">function</tok></cons> <cons cat="PP" ref="i15" null="RNR"></cons></cons><cons cat="PP" id="i15"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">expressed</tok> <tok cat="NNS">genes</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">lymphocyte</tok> <tok cat="NN">differentiation</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">disorders</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">discussed</tok><cons cat="NP" ref="i14" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
971526501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Characterization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="JJ">differential</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">T</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NK</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><cons cat="NP" id="i1"><tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
971526502@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NNS">Cytokines</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-6</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-7</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-12</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-15</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">key</tok> <tok cat="NNS">regulators</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">T</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NK</tok></cons></cons> <tok cat="NN">cell</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">precise</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">give</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBP">rise</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">differential</tok> <tok cat="NNS">activities</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">clear</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@16-12-2010
971526503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Recent</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">reveal</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">family</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NNS">transducers</tok> <tok cat="CC">and</tok> <tok cat="NNS">activators</tok> <tok cat="IN">of</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">STATs</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">directly</tok></cons> <cons cat="VP"><tok cat="VB">mediate</tok> <cons cat="NP"><tok cat="JJ">many</tok> <tok cat="NN">cytokine</tok> <tok cat="NNS">signals</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
971526504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">analyzed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">STATs</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">T</tok> <cons cat="NP" ref="i4" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NK</tok> <cons cat="NP" ref="i4" null="RNR"></cons></cons><cons cat="NP" id="i4"><tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">variety</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NNS">cytokines</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
971526505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-12</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><tok cat="NN">STAT4</tok></cons> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">freshly</tok> <tok cat="VBN">isolated</tok></cons> <tok cat="JJ">primary</tok> <tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <tok cat="RB">not</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">lack</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-12</tok> <tok cat="NN">receptor</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBG">resting</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
971526506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">IL-4</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NN">C</tok> <tok cat="NN">epsilon</tok> <tok cat="NN">GAS</tok> <tok cat="JJ">DNA-protein</tok> <tok cat="NN">binding</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">T</tok> <cons cat="NP" ref="i13" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NK</tok> <cons cat="NP" ref="i13" null="RNR"></cons></cons><cons cat="NP" id="i13"><tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
971526507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">IL-4</tok> <tok cat="NN">costimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">IL-12</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">alter</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">own</tok> <cons cat="NP"><tok cat="JJ">preferential</tok> <tok cat="JJ">GAS-like</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NNS">patterns</tok></cons></cons> <cons cat="SBAR" adv="TMP"><tok cat="WRB">when</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">C</tok> <tok cat="NN">epsilon-</tok><cons cat="NP" ref="i7" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Fc</tok> <tok cat="NN">gamma</tok> <tok cat="NN">RI-</tok><cons cat="NP" ref="i7" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">SIE</tok> <tok cat="NN">GAS</tok> <cons cat="NP" ref="i7" null="RNR"></cons></cons><cons cat="NP" id="i7"><tok cat="NN">motif</tok></cons></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="NN">oligonucleotide</tok> <tok cat="NNS">probes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">compared</tok><cons cat="NP" ref="i6" null="NONE"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">GAS-like</tok> <tok cat="JJ">DNA-protein</tok> <tok cat="NN">binding</tok> <tok cat="NNS">complexes</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-12</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">highly</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">selective</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">represents</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok> <tok cat="JJ">important</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">determining</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">gene</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@65@@oe@16-12-2010
971526508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">IL-6</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">synergistically</tok></cons> <cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">homo-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">heterodimerized</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT1</tok> <tok cat="NN">alpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT3</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">NK</tok> <cons cat="NP" ref="i8" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <cons cat="NP" ref="i8" null="RNR"></cons></cons><cons cat="NP" id="i8"><tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="VBN">reported</tok> <tok cat="NN">synergism</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">modulating</tok> <cons cat="NP"><tok cat="NN">perforin</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
971526509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">further</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">IL-2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><tok cat="CD">-7</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><tok cat="CD">-15</tok></cons></cons> <cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">multiple</tok> <tok cat="NN">STAT</tok> <tok cat="NNS">proteins</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT5a</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">STAT5b</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">STAT1</tok> <tok cat="NN">alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">STAT3</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">another</tok> <tok cat="JJ">unidentified</tok> <tok cat="NN">Fc</tok> <tok cat="NN">gamma</tok> <tok cat="NN">RI</tok> <tok cat="NN">GAS</tok> <tok cat="NN">DNA-binding</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
971526510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="VBN">activated</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT5a</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT5b</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><cons cat="NP" id="i2"><tok cat="NNS">proteins</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">form</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">distinct</tok> <tok cat="NN">Fc</tok> <tok cat="NN">gamma</tok> <tok cat="NN">RI</tok> <tok cat="NN">GAS</tok> <tok cat="NN">binding</tok> <tok cat="NNS">patterns</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">T</tok> <cons cat="NP" ref="i10" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NK</tok> <cons cat="NP" ref="i10" null="RNR"></cons></cons><cons cat="NP" id="i10"><tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">roles</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">regulation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
971526511@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">evidence</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">differential</tok> <tok cat="NNS">responses</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons> <cons cat="VP"><tok cat="VBN">seen</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">NK</tok> <cons cat="NP" ref="i12" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP" null="QSTN"></cons><tok cat="NN">T</tok> <cons cat="NP" ref="i12" null="RNR"></cons></cons><cons cat="NP" id="i12"><tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">direct</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">cytokines</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">direct</tok> <tok cat="NN">result</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">distinct</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
971583801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">replication</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">AZT</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
971583802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">small</tok> <tok cat="NN">molecule</tok> <tok cat="NN">S9a</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">established</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor-alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">inhibitor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Canventol</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">replacement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">isopropylidine</tok> <tok cat="NN">group</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">phenyl</tok> <tok cat="NN">ring</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
971583803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">S9a</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="QP"><tok cat="CD">10</tok> <tok cat="TO">to</tok> <tok cat="CD">100</tok></cons> <cons cat="NP"><tok cat="NN">nM</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">production</tok></cons> <cons cat="ADVP"><cons cat="ADVP"><tok cat="RB">as</tok> <tok cat="RB">potently</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">3'-azido-3'-deoxythymidine</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AZT</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="JJ">reverse</tok> <tok cat="NN">transcriptase</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
971583804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">S9a</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">AZT</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">combination</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="JJ">noncytoxic</tok> <tok cat="NNS">concentrations</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">replication</tok> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RBR">more</tok> <tok cat="IN">than</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">additive</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">substantially</tok></cons> <cons cat="VP"><tok cat="VBD">prolonged</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">appearance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">virus</tok></cons> <cons cat="PP" syn="COOD"><tok cat="CC">both</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="RB">acutely</tok> <tok cat="JJ">infected</tok> <tok cat="JJ">CD4+</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SupT</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">culture</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">PBMCs</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBN">infected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">primary</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">isolate</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@54@@oe@16-12-2010
971583805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">S9a</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="JJ">promoter-driven</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok> <tok cat="NN">activity</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@16-12-2010
971583806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">proposed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">antiviral</tok> <tok cat="NN">action</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">S9a</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="PP" role="PRD"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">host</tok> <tok cat="NN">cell</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">blocking</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">Tat-induced</tok> <tok cat="JJ">tar-independent</tok> <tok cat="NN">loop</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">decreases</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">downstream</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LTR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
971583807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">S9a</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">superior</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NN">generation</tok> <tok cat="JJ">compound</tok></cons> <cons cat="NP"><tok cat="NN">Canventol</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">superior</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">natural</tok> <tok cat="NN">compound</tok></cons> <cons cat="NP"><tok cat="NN">sarcophytol</tok> <tok cat="NN">A</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">demonstrating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">further</tok> <tok cat="JJ">structure-based</tok> <tok cat="NN">enhancement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">potency</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">compounds</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">feasible</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
971583808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">therapeutic</tok> <tok cat="NN">approach</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="NN">AIDS</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">application</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">drugs</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP" id="i6"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP" id="i7"><tok cat="DT">a</tok> <tok cat="JJ">cellular</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons map="i6" cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">other</tok></cons> <cons map="i7" cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">viral</tok> <tok cat="NN">target</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">approach</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">problem</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">frequent</tok> <tok cat="NN">emergence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">resistant</tok> <tok cat="NNS">variants</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">combinations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">drugs</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">target</tok> <cons cat="ADVP"><tok cat="RB">only</tok></cons> <cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@49@@oe@16-12-2010
971596601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Specific</tok> <tok cat="NN">glucocorticoid</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">motor</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
971596602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">studied</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">dissociation</tok> <tok cat="NN">constant</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">isolated</tok> <tok cat="JJ">human</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">blood</tok> <tok cat="NNS">concentrations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">hormones</tok></cons> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="JJ">hypothalamic-hypophyseal-adrenocortical</tok> <tok cat="NN">system</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok> <tok cat="JJ">experimental</tok> <tok cat="NNS">series</tok></cons><tok cat="COLON">:</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">normal</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">17</tok> <tok cat="NNS">subjects</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="VBN">decreased</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">10</tok> <tok cat="NNS">subjects</tok><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">360-d</tok> <tok cat="JJ">head-down</tok> <tok cat="NN">bed</tok> <tok cat="NN">rest</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="VBN">increased</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="PRN"><tok cat="CD">8</tok> <tok cat="NNS">subjects</tok><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">physical</tok> <tok cat="NN">exercise</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">bicycle</tok> <tok cat="NN">ergometer</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">motor</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@66@@oe@16-12-2010
971596603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NN">series</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">dissociation</tok> <tok cat="NN">constant</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">depend</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">season</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">age</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">subjects</tok></cons></cons></cons></cons> <tok cat="CC">nor</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">cortisol</tok> <tok cat="NNS">concentrations</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">blood</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
971596604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">second</tok> <tok cat="NN">series</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">following</tok></cons><tok cat="COLON">:</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">end</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NN">month</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">bed</tok> <tok cat="NN">rest</tok></cons></cons></cons></cons></cons></cons> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="NN">glucocorticoid</tok> <tok cat="NNS">receptors</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">receptor</tok> <tok cat="NN">affinity</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP"><tok cat="VBD">increased</tok></cons></cons><tok cat="COLON">;</tok> <cons cat="S" syn="COOD"><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">beginning</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">third</tok> <tok cat="NN">month</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">bed</tok> <tok cat="NN">rest</tok></cons></cons></cons></cons></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">specific</tok> <tok cat="NN">glucocorticoid</tok> <tok cat="NN">binding</tok></cons> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP"><tok cat="VBD">decreased</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">circadian</tok> <tok cat="NNS">rhythms</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">adrenocorticotropin</tok> <cons cat="PP" ref="i1" null="RNR"></cons></cons><tok cat="CC">and</tok> <tok cat="NN">cortisol</tok> <cons cat="PP" id="i1"><tok cat="IN">in</tok> <tok cat="NN">blood</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">varied</tok> <cons cat="ADVP"><tok cat="RB">markedly</tok></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S" syn="COOD"><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">end</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">sixth</tok> <tok cat="NN">month</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">bed</tok> <tok cat="NN">rest</tok></cons></cons></cons></cons></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <tok cat="NN">glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons></cons> <cons cat="VP"><tok cat="VBD">returned</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">prebed</tok> <tok cat="NN">rest</tok> <tok cat="NNS">levels</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="QP" null="QSTN"></cons><tok cat="NN">dissociation</tok> <tok cat="NN">constant</tok></cons> <cons cat="VP"><tok cat="VBD">decreased</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@82@@oe@16-12-2010
971596605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">third</tok> <cons cat="NP"><tok cat="NN">series</tok> <cons cat="NP"><tok cat="JJ">physical</tok> <tok cat="NNS">exercises</tok></cons></cons></cons></cons> <cons cat="SBAR" role="SBJ"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">hypothalamic-hypophyseal-adrenocortical</tok> <tok cat="NN">system</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">maximal</tok> <cons cat="NP"><tok cat="JJ">physical</tok> <tok cat="NNS">exercises</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">prolonged</tok> <cons cat="NP"><tok cat="JJ">submaximal</tok> <tok cat="NNS">exercises</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">70</tok><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">maximal</tok> <tok cat="NN">oxygen</tok> <tok cat="NN">uptake</tok></cons></cons></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">led</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">significant</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <tok cat="NN">glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">dissociation</tok> <tok cat="NN">constant</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@16-12-2010
971596606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">decrease</tok> <cons cat="PP" ref="i3" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok> <cons cat="PP" ref="i3" null="RNR"></cons></cons><cons cat="PP" id="i3"><tok cat="IN">of</tok> <tok cat="JJ">human</tok> <tok cat="NN">motor</tok> <tok cat="NN">activity</tok></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">significant</tok> <tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">glucocorticoid</tok> <tok cat="NN">binding</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" id="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">influenced</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBG">circulating</tok> <tok cat="NN">hormone</tok> <tok cat="NNS">concentrations</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">blood</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">some</tok> <tok cat="JJ">other</tok> <tok cat="NNS">factors</tok></cons> <cons cat="VP"><tok cat="VBN">affected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <tok cat="JJ">physical</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
971660001@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Retinoic</tok> <tok cat="NN">acid</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CD40</tok></cons> <tok cat="CC">+</tok> <cons cat="NP"><tok cat="JJ">interleukin-4-mediated</tok> <tok cat="NN">IgE</tok> <tok cat="NN">production</tok></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
971660002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">elucidate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">anti-CD40</tok></cons> <tok cat="CC">+</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-4</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-4</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="JJ">-mediated</tok> <tok cat="NN">B-cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="QP"><tok cat="CD">10(-12)</tok> <tok cat="TO">to</tok> <tok cat="CD">10(-6)</tok></cons> <tok cat="NN">mol/L</tok> <tok cat="NN">RA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">studied</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">anti-CD40</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">1</tok> <tok cat="NN">microgram/mL</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">+</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">IL-4</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">5</tok> <tok cat="NN">ng/mL</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="JJ">-mediated</tok> <tok cat="NN">proliferation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Ig</tok> <tok cat="NN">synthesis</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">PBMC</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">donors</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@64@@oe@16-12-2010
971660003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="NN">Anti-CD40</tok> <tok cat="CC">+</tok> <tok cat="JJ">IL-4-mediated</tok> <tok cat="NN">proliferation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">PBMC</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">RA</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">dose-dependent</tok> <tok cat="NN">manner</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">maximal</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP" id="i12"><tok cat="CD">62</tok><tok cat="NN">%</tok> <tok cat="CC">+/-</tok> <tok cat="CD">5</tok><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">PBMC</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" id="i13"><tok cat="CD">55</tok><tok cat="NN">%</tok> <tok cat="CC">+/-</tok> <tok cat="CD">4.4</tok><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">all-trans</tok> <tok cat="NN">RA</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons map="i12" cat="NP"><tok cat="CD">58</tok><tok cat="NN">%</tok> <tok cat="CC">+/-</tok> <tok cat="CD">6.7</tok><tok cat="NN">%</tok></cons> <tok cat="CC">and</tok> <cons map="i13" cat="NP"><tok cat="CD">51</tok><tok cat="NN">%</tok> <tok cat="CC">+/-</tok> <tok cat="CD">4.7</tok><tok cat="NN">%</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">respectively</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">13-cis</tok> <tok cat="NN">RA</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@60@@oe@16-12-2010
971660004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">IgE</tok> <tok cat="NN">synthesis</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">even</tok> <tok cat="RBR">more</tok> <tok cat="RB">markedly</tok></cons> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">RA</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">starting</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">concentrations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="JJR">&gt;</tok><cons cat="NP"><tok cat="CD">10(-14)</tok> <tok cat="NN">mol/L</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="QSTN"></cons><cons cat="VP"><tok cat="JJR">&gt;</tok><cons cat="NP"><tok cat="CD">10(-10)</tok> <tok cat="NN">mol/L</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NNS">PBMC</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
971660005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Maximal</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IgE</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="PP" role="PRD"><tok cat="IN">at</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">10(-8)</tok></cons> <tok cat="NN">mol/L</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">all-trans</tok> <tok cat="NN">RA</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">94</tok><tok cat="NN">%</tok> <tok cat="CC">+/-</tok> <tok cat="CD">1.8</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="QP"><tok cat="CD">96</tok><tok cat="NN">%</tok> <tok cat="CC">+/-</tok> <tok cat="CD">3.2</tok><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">13-cis</tok> <tok cat="NN">RA</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
971660006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Low</tok> <tok cat="NNS">concentrations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">RA</tok></cons> <cons cat="VP"><tok cat="VBG">inhibiting</tok> <cons cat="NP"><tok cat="NN">IgE</tok> <tok cat="NN">synthesis</tok></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="QP"><tok cat="CD">10(-10)</tok></cons> <tok cat="NN">mol/L</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">affected</tok> <cons cat="NP" syn="COOD"><tok cat="CC">neither</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">proliferation</tok></cons> <tok cat="CC">nor</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IgA</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IgG</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IgM</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
971660007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Elucidation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IgE</tok> <tok cat="NN">production</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">shows</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">epsilon</tok> <tok cat="NN">germline</tok> <tok cat="NN">transcription</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">decreased</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">RA</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interferon-gamma</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">enhanced</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">RA</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
971660008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">differentiate</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">RA</tok> <tok cat="NN">effect</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">RA</tok> <tok cat="NNS">receptors</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">beta</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">gamma</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">RAR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">reverse</tok> <tok cat="JJ">transcriptase-polymerase</tok> <tok cat="NN">chain</tok> <tok cat="NN">reaction</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RT-PCR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
971660009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">unstimulated</tok> <tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">mRNA</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">RA</tok> <tok cat="NN">receptor</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">beta</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">gamma</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
971660010@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">retinoids</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NN">receptor</tok> <tok cat="NN">binding</tok> <tok cat="NN">specificity</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CD336</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CD437</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CD2019</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CD367</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="JJ">dose-dependent</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IgE</tok> <tok cat="NN">synthesis</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">all</tok> <tok cat="CD">four</tok> <tok cat="NNS">derivates</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RBS">most</tok></cons> <cons cat="VP"><tok cat="VBN">marked</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">RA</tok></cons> <cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">alpha</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NN">specificity</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
971660011@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">Taken</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">together</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBZ">shows</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">RA</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IgE</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">anti-CD40</tok> <tok cat="CC">+</tok> <tok cat="JJ">IL-4-stimulated</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
971660012@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Copyright</tok> <tok cat="CD">1998</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNP">The</tok> <tok cat="NNP">American</tok> <tok cat="NNP">Society</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNP">Hematology</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010