1007027401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Nuclear</tok> <tok cat="NN">factor-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">rheumatic</tok> <tok cat="NNS">diseases</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">preliminary</tok> <tok cat="NN">report</tok></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@16-12-2010
1007027402@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">OBJECTIVE</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B/Rel</tok> <tok cat="NN">family</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">regulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">many</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">immune</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">inflammatory</tok></cons></cons> <tok cat="NN">response</tok></cons></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">level</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@16-12-2010
1007027403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">aim</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="S" role="PRD"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="JJ">distinctive</tok> <tok cat="NNS">patterns</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">seen</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">forms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">joint</tok> <tok cat="NN">disease</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1007027404@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">METHODS</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">nucleoproteins</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">purified</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">synovial</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">peripheral</tok></cons></cons> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">various</tok> <tok cat="JJ">chronic</tok> <tok cat="JJ">rheumatic</tok> <tok cat="NNS">diseases</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="CD">12</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="CD">four</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">rheumatoid</tok> <tok cat="NN">arthritis</tok></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">five</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NNS">spondyloarthropathies</tok></cons></cons></cons><tok cat="COLON">;</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">osteoarthritis</tok></cons></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
1007027405@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">RESULTS</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons> <cons cat="VP"><tok cat="VBD">disclosed</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">specific</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="VP"><tok cat="VBN">bound</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="JJ">specific</tok></cons> <tok cat="JJ">32P-labelled</tok> <tok cat="NN">oligonucleotide</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">nucleoproteins</tok></cons> <cons cat="VP"><tok cat="VBN">extracted</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">purified</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">synovial</tok> <tok cat="NN">fluid</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">rheumatoid</tok> <tok cat="NN">arthritis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
1007027406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="VP"><tok cat="VBD">consisted</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p50/p50</tok> <tok cat="NNS">homodimers</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p50/p65</tok> <tok cat="NNS">heterodimers</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
1007027407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="VBN">Increased</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">DNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">synovial</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="JJ">relative</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1007027408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">non-rheumatoid</tok> <tok cat="NN">arthritis</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">synovial</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">exclusively</tok></cons> <tok cat="VBN">mediated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">p50/p50</tok> <tok cat="NNS">homodimers</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1007027409@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">CONCLUSION</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="ADVP"><tok cat="RB">Overall</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">central</tok> <tok cat="NN">part</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBG">infiltrating</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">chronic</tok> <tok cat="JJ">rheumatoid</tok> <tok cat="NN">arthritis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1007027410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">qualitatively</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">different</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">rheumatoid</tok> <tok cat="JJ">synovial</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">forms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">non-rheumatoid</tok> <tok cat="NN">arthritis</tok></cons></cons> <tok cat="LRB">(</tok><cons cat="PP"><tok cat="IN">for</tok> <tok cat="NN">example</tok><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">osteoarthritis</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNS">spondyloarthropathies</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
1007095301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">High</tok> <tok cat="NN">frequency</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">germ-line</tok> <tok cat="NN">BRCA2</tok> <tok cat="NNS">mutations</tok></cons></cons> <cons cat="PP"><tok cat="IN">among</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Hungarian</tok> <tok cat="JJ">male</tok> <tok cat="NN">breast</tok> <tok cat="NN">cancer</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP"><tok cat="NN">family</tok> <tok cat="NN">history</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1007095302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">contribution</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">BRCA1</tok> <tok cat="CC">and</tok> <tok cat="NN">BRCA2</tok> <tok cat="NNS">mutations</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathogenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">male</tok> <tok cat="NN">breast</tok> <tok cat="NN">cancer</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNP">Hungary</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">country</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJS">highest</tok> <tok cat="JJ">male</tok> <tok cat="NN">breast</tok> <tok cat="NN">cancer</tok> <tok cat="NN">mortality</tok> <tok cat="NNS">rates</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">continental</tok> <tok cat="NN">Europe</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">series</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">18</tok> <tok cat="JJ">male</tok> <tok cat="NN">breast</tok> <tok cat="NN">cancer</tok> <tok cat="NNS">patients</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">gynecomastia</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">germ-line</tok> <tok cat="NNS">mutations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">BRCA1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">BRCA2</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@57@@oe@16-12-2010
1007095303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="DT">no</tok> <tok cat="NN">germ-line</tok> <tok cat="NN">BRCA1</tok> <tok cat="NN">mutation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok><cons cat="NP" ref="i2" null="NONE"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="CD">6</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">18</tok> <tok cat="JJ">male</tok> <tok cat="NN">breast</tok> <tok cat="NN">cancer</tok> <tok cat="NNS">cases</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">33</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">carried</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">truncating</tok> <tok cat="NNS">mutations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">BRCA2</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1007095304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Unexpectedly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">none</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRP">them</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">reported</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">family</tok> <tok cat="NN">history</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">breast/ovarian</tok> <tok cat="NN">cancer</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
1007095305@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="QP"><tok cat="CD">Four</tok> <tok cat="IN">of</tok> <tok cat="CD">six</tok></cons> <tok cat="JJ">truncating</tok> <tok cat="NNS">mutations</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">novel</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="CD">two</tok> <tok cat="NNS">mutations</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">recurrent</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1007095306@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">Four</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">22</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">family</tok> <tok cat="NN">history</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">breast/ovarian</tok> <tok cat="NN">cancer</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="QP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok> <tok cat="CD">one</tok></cons> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">first-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">second-degree</tok></cons></cons> <tok cat="JJ">relative</tok></cons></cons></cons></cons><tok cat="COLON">;</tok> <tok cat="RB">however</tok><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="DT">no</tok> <tok cat="NN">BRCA2</tok> <tok cat="NN">mutation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">among</tok> <cons cat="NP"><tok cat="PRP">them</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1007095307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="DT">No</tok> <tok cat="NN">mutation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">either</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">genes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">gynecomastias</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
1007095308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">strong</tok> <tok cat="JJ">genetic</tok> <tok cat="NN">component</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">male</tok> <tok cat="NN">breast</tok> <tok cat="NN">cancer</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNP">Hungary</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1007206801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">RFX-B</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">gene</tok></cons> <cons cat="ADJP"><tok cat="JJ">responsible</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RBS">most</tok> <tok cat="JJ">common</tok></cons> <tok cat="NN">cause</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">bare</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NN">syndrome</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">immunodeficiency</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="VBN">published</tok> <tok cat="NN">erratum</tok></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Immunity</tok></cons> <cons cat="NP" adv="TMP"><tok cat="CD">1999</tok> <tok cat="NNP">Mar</tok></cons><tok cat="COLON">;</tok><cons cat="NP"><tok cat="CD">10</tok><tok cat="LRB">(</tok><tok cat="CD">3</tok><tok cat="RRB">)</tok></cons><tok cat="COLON">:</tok><cons cat="NP"><tok cat="CD">399</tok></cons></cons></cons></cons></cons><tok cat="RRB">]</tok></cons></cons></cons>@@@@1@37@@oe@16-12-2010
1007206802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">bare</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NN">syndrome</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">BLS</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">characterized</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">absence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">transcription</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">humoral-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">cellular-mediated</tok></cons></cons> <tok cat="JJ">immune</tok> <tok cat="NNS">responses</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">foreign</tok> <tok cat="NNS">antigens</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1007206803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">Three</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">four</tok> <tok cat="NN">BLS</tok> <tok cat="JJ">genetic</tok> <tok cat="NN">complementation</tok> <tok cat="NNS">groups</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><tok cat="NNS">defects</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">RFX</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1007206804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">purified</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">RFX</tok> <tok cat="NN">complex</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">sequenced</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="CD">three</tok> <tok cat="NNS">subunits</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1007206805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">sequence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJS">smallest</tok> <tok cat="NN">subunit</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">describes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="NN">gene</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">termed</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NN">RFX-B</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1007206806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="NN">RFX-B</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">complements</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">predominant</tok> <tok cat="NN">BLS</tok> <tok cat="NN">complementation</tok> <tok cat="NN">group</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">group</tok> <tok cat="NN">B</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">mutant</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">BLS</tok> <tok cat="NN">group</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1007206807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="JJ">known</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">domain</tok></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBZ">does</tok> <cons cat="VP"><tok cat="VB">contain</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok> <tok cat="NN">ankyrin</tok> <tok cat="NNS">repeats</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" id="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">likely</tok> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">protein-protein</tok> <tok cat="NNS">interactions</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1007207801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Alternative</tok> <tok cat="NN">polyadenylation</tok> <tok cat="NNS">events</tok></cons> <cons cat="VP"><tok cat="VBP">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-ATc</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">effector</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1007207802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">NF-ATc</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">synthesized</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="CD">three</tok> <tok cat="JJ">prominent</tok> <tok cat="NNS">isoforms</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
1007207803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok></cons> <cons cat="VP"><tok cat="VBP">differ</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">length</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">C</tok> <tok cat="JJ">terminal</tok> <tok cat="NNS">peptides</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">mode</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">synthesis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1007207804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="JJ">Due</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">switch</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">use</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" id="i2"><tok cat="DT">a</tok> <tok cat="JJ">3'</tok> <tok cat="NN">polyA</tok> <tok cat="NN">site</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons map="i2" cat="NP"><tok cat="DT">a</tok> <tok cat="RBR">more</tok> <tok cat="JJ">proximal</tok> <tok cat="NN">polyA</tok> <tok cat="NN">site</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">NF-ATc</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">switches</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">synthesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="JJR">longer</tok> <tok cat="NNS">isoforms</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">naive</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">short</tok> <tok cat="NN">isoform</tok> <tok cat="NN">A</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">effector</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
1007207805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="ADJP"><tok cat="JJ">relative</tok> <tok cat="JJ">low</tok></cons> <tok cat="NN">binding</tok> <tok cat="NN">affinity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cleavage</tok> <tok cat="NN">stimulation</tok> <tok cat="NN">factor</tok> <tok cat="NN">CstF-64</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">proximal</tok> <tok cat="NN">polyA</tok> <tok cat="NN">site</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">seems</tok> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">neglect</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">naive</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1007207806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="JJ">alternative</tok> <tok cat="NN">polyadenylation</tok> <tok cat="NNS">events</tok></cons> <cons cat="VP"><tok cat="VBP">ensure</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">rapid</tok> <tok cat="NN">accumulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">concentrations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-ATc</tok></cons></cons></cons></cons></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">necessary</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">exceed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">critical</tok> <tok cat="JJ">threshold</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-ATc</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">effector</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1007249701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">N-acetyl-L-cysteine</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">responses</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">dendritic</tok> <tok cat="NN">cell</tok> <tok cat="NN">level</tok></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">association</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">inhibition</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@16-12-2010
1007249702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" role="SBJ"><tok cat="NN">N-acetyl-L-cysteine</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NAC</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">antioxidant</tok> <tok cat="NN">molecule</tok></cons> <cons cat="VP"><tok cat="JJ">endowed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">immunomodulatory</tok> <tok cat="NNS">properties</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
1007249703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">investigate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NAC</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok> <tok cat="NN">phase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">responses</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i1" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">analyzed</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">action</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">dendritic</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">DC</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">adherent</tok> <tok cat="NN">PBMC</tok></cons> <cons cat="VP"><tok cat="VBN">cultured</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-4</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">granulocyte-macrophage</tok> <tok cat="NN">CSF</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
1007249704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">first</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NAC</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">constitutive</tok> <cons cat="NP" ref="i11" null="RNR"></cons></cons><cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">LPS-induced</tok> <cons cat="NP" ref="i11" null="RNR"></cons></cons></cons><cons cat="NP" id="i11"><tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1007249705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">parallel</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i2" role="SBJ"><tok cat="NN">NAC</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">down-regulate</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">DC</tok></cons></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">surface</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HLA-DR</tok><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD86</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">B7-2</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <tok cat="NN">CD40</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons> <cons cat="PP" syn="COOD"><tok cat="CC">both</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">basal</tok> <tok cat="NN">state</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP" adv="TMP"><tok cat="IN">upon</tok> <cons cat="NP"><tok cat="NN">LPS</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
1007249706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NAC</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">DC</tok> <tok cat="NNS">responses</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">CD40</tok> <tok cat="NN">engagement</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
1007249707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NAC</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="PP" role="PRD"><tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">nonspecific</tok> <tok cat="NN">toxicity</tok></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i3" role="SBJ"><tok cat="CC">neither</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">viability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DC</tok></cons></cons></cons> <tok cat="CC">nor</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <cons cat="ADJP"><tok cat="NN">mannose</tok> <tok cat="JJ">receptor-mediated</tok></cons> <tok cat="NN">endocytosis</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">modified</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">NAC</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1007249708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NAC</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">MLR</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">naive</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">allogeneic</tok> <tok cat="NN">DC</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">profound</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">alloreactive</tok> <tok cat="NNS">responses</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" id="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">attributed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">defect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DC</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="JJ">APC-independent</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">responses</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">NAC</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@16-12-2010
1007249709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Altogether</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NAC</tok></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">impair</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">generation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">immune</tok> <tok cat="NNS">responses</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">humans</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NN">action</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">DC</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1007252901@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="NN">NF-ATc</tok> <tok cat="NNS">isoforms</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">differentially</tok></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i1" null="NONE"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i1" null="NONE"></cons></cons></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">T</tok> <tok cat="CC">and</tok> <tok cat="NN">mast</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1007252902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">NF</tok> <tok cat="IN">of</tok> <tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-AT</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">denotes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">family</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">regulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">activation-dependent</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">many</tok> <tok cat="RB">immunologically</tok> <tok cat="JJ">important</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1007252903@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="QP"><tok cat="IN">At</tok> <tok cat="JJS">least</tok> <tok cat="CD">four</tok></cons> <tok cat="JJ">distinct</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBP">encode</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">various</tok> <tok cat="NN">family</tok> <tok cat="NNS">members</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NNS">isoforms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">as</tok> <tok cat="RB">well</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1007252904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBG">overlapping</tok> <tok cat="NN">expression</tok> <tok cat="NNS">patterns</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">similar</tok> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">binding</tok> <tok cat="CC">and</tok> <tok cat="JJ">trans-activation</tok> <tok cat="NNS">activities</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NN">promoter</tok> <tok cat="NNS">elements</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">NF-AT-regulated</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">some</tok> <tok cat="NN">redundancy</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1007252905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">phenotypic</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">NF-AT-deficient</tok> <tok cat="NNS">mice</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">supports</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">idea</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">tissue-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">gene- specific</tok></cons></cons> <tok cat="NNS">functions</tok></cons> <cons cat="ADVP"><tok cat="RB">as</tok> <tok cat="RB">well</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1007252906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">characterized</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok> <tok cat="NNS">cDNAs</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">mast</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1007252907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">majority</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">clones</tok></cons> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" null="NONE"></cons></cons></cons></cons></cons><cons cat="VP"><tok cat="VBP">correspond</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NN">NF-ATc</tok> <tok cat="NNS">isoforms</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">differ</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">amino-terminal</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1007252908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Despite</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">minimal</tok> <tok cat="NNS">discrepancies</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBG">coding</tok> <tok cat="NN">region</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><tok cat="JJ">striking</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">tissue-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="NN">cell</tok> <tok cat="JJ">type-specific</tok></cons></cons> <tok cat="NNS">differences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">isoform</tok> <tok cat="NN">expression</tok> <tok cat="NNS">patterns</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1007252909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Detection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-ATc.alpha</tok> <tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">strictly</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">activation</tok> <tok cat="NNS">signals</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="CC">both</tok> <tok cat="NN">T</tok> <tok cat="CC">and</tok> <tok cat="NN">mast</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1007252910@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">beta</tok> <tok cat="NN">isoform</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">very</tok> <tok cat="JJ">low</tok></cons> <tok cat="JJ">constitutive</tok> <tok cat="NNS">levels</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <tok cat="VBN">up-regulated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">mast</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok> <tok cat="NNS">signals</tok></cons> <cons cat="VP"><tok cat="VBN">delivered</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">FcepsilonRI</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="NN">calcium</tok> <tok cat="NNS">ionophores</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
1007252911@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">another</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">regulation</tok></cons></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-AT</tok> <tok cat="NN">family</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">cell</tok> <tok cat="JJ">type-specific</tok></cons> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1007443201@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">direct</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">adaptor</tok> <tok cat="NN">protein</tok> <tok cat="NN">Cbl-b</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">kinase</tok> <tok cat="NN">zap-70</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">positive</tok> <tok cat="NN">signal</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1007443202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Engagement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">receptor</tok> <tok cat="LRB">(</tok><tok cat="NN">TCR</tok><tok cat="RRB">)</tok><tok cat="JJ">-CD3</tok> <tok cat="NN">complex</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">rapid</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">activities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Src-family</tok> <tok cat="CC">and</tok> <tok cat="NN">Syk/Zap-70-family</tok> <tok cat="NNS">kinases</tok></cons></cons></cons></cons></cons> <cons cat="PRN" syn="COOD"><cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP"><tok cat="CD">1</tok></cons><tok cat="RRB">]</tok></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP"><tok cat="CD">2</tok></cons><tok cat="RRB">]</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1007443203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="DT">These</tok> <tok cat="VBN">activated</tok> <tok cat="NNS">kinases</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">then</tok></cons> <cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">multiple</tok> <tok cat="JJ">intracellular</tok> <tok cat="NNS">proteins</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="ADVP" adv="TMP"><tok cat="RB">eventually</tok></cons> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1007443204@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">One</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">prominent</tok> <tok cat="NNS">substrates</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">kinases</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">the</tok> <tok cat="NN">adaptor</tok> <tok cat="NN">protein</tok> <tok cat="NN">Cbl</tok></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP"><tok cat="CD">3</tok></cons><tok cat="RRB">]</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">recent</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Cbl</tok></cons> <cons cat="ADVP"><tok cat="RB">negatively</tok></cons> <cons cat="VP"><tok cat="VBZ">regulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">upstream</tok> <tok cat="NNS">kinases</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Syk</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Zap-70</tok></cons></cons></cons></cons> <cons cat="PRN" syn="COOD"><cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP"><tok cat="CD">4</tok></cons><tok cat="RRB">]</tok></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP"><tok cat="CD">5</tok></cons><tok cat="RRB">]</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
1007443205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="NN">Cbl-b</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">homologue</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Cbl</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">widely</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">many</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">tissues</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PRN" syn="COOD"><cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP"><tok cat="CD">6</tok></cons><tok cat="RRB">]</tok></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP"><tok cat="CD">7</tok></cons><tok cat="RRB">]</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1007443206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Cbl-b</tok></cons> <cons cat="VP"><tok cat="VBZ">undergoes</tok> <cons cat="NP"><tok cat="JJ">rapid</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">TCR</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons></cons> <cons cat="PRN" syn="COOD"><cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP"><tok cat="CD">8</tok></cons><tok cat="RRB">]</tok></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP"><tok cat="CD">9</tok></cons><tok cat="RRB">]</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1007443207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Cbl-b</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unclear</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">however</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
1007443208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">overexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Cbl-b</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">constitutive</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="IN">of</tok> <tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NFAT</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1007443209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">loss-of-function</tok> <tok cat="NN">mutation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Cbl-b</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">disrupted</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Cbl-b</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Zap-70</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">nearly</tok> <tok cat="RB">completely</tok></cons> <tok cat="VBD">abrogated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">Cbl-b-mediated</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFAT</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1007443210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Unlike</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">proposed</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Cbl</tok></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">negative</tok> <tok cat="NN">regulator</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">Cbl</tok> <tok cat="NN">homologue</tok> <tok cat="NN">Cbl-b</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">positive</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">signaling</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RBS">most</tok> <tok cat="RB">likely</tok></cons> <tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">direct</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">upstream</tok> <tok cat="NN">kinase</tok> <tok cat="NN">Zap-70</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
1007564501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Bacterial</tok> <tok cat="NN">lipopolysaccharide</tok></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappaB</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="NN">interleukin-1</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">mediators</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">cultured</tok> <tok cat="JJ">human</tok> <tok cat="JJ">dermal</tok> <tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NNS">phagocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1007564502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="ADJP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Bacterial</tok> <tok cat="NN">lipopolysaccharide</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LPS</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="JJ">-mediated</tok></cons> <tok cat="JJ">immune</tok> <tok cat="NNS">responses</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathogenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">Gram-negative</tok> <tok cat="JJ">bacteria-induced</tok> <tok cat="NN">sepsis</tok> <tok cat="NN">syndrome</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1007564503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">thought</tok> <cons cat="S"><cons cat="NP" ref="i1" id="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">release</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">LPS-responsive</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">critical</tok> <tok cat="NN">step</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">endotoxic</tok> <tok cat="NNS">effects</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1007564504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">role</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">involvement</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">signal</tok> <tok cat="NN">transducer</tok> <tok cat="NNS">molecules</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">LPS</tok> <tok cat="NN">signaling</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">dermal</tok> <tok cat="NN">microvessel</tok> <tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HDMEC</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">THP-1</tok> <tok cat="JJ">monocytic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
1007564505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">LPS</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HDMEC</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">THP-1</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">initiated</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP"><tok cat="NN">IL-1</tok> <tok cat="JJ">receptor-like</tok></cons> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">signaling</tok> <tok cat="NN">cascade</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1007564506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">cotransfection</tok> <tok cat="NNS">experiments</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">dominant</tok> <tok cat="JJ">negative</tok> <tok cat="NNS">mutants</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-1</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">MyD88</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IRAK</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IRAK2</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TRAF6</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADJP"><tok cat="NN">IL-1-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">LPS-induced</tok></cons></cons> <tok cat="NN">NF-kappaB-luciferase</tok> <tok cat="NN">activity</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
1007564507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="JJ">LPS-induced</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">dominant</tok> <tok cat="JJ">negative</tok> <tok cat="NN">mutant</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TRAF2</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" id="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">TNF</tok> <tok cat="NN">signaling</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1007564508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="JJ">LPS-induced</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">NF-kappaB-responsive</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="NN">receptor</tok> <tok cat="NN">antagonist</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1007564509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i7" role="SBJ"><cons cat="NP"><tok cat="NN">TLR2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TLR4</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">surface</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HDMEC</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">THP-1</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1007564510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok> <tok cat="NN">molecule</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">LPS</tok> <tok cat="NN">receptor</tok> <tok cat="NN">complex</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">belong</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-1</tok> <tok cat="JJ">receptor/toll-like</tok> <tok cat="NN">receptor</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TLR</tok></cons><tok cat="RRB">)</tok></cons> <tok cat="JJ">super</tok> <tok cat="NN">family</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">LPS</tok> <tok cat="NN">signaling</tok> <tok cat="NN">cascade</tok></cons> <cons cat="VP"><tok cat="VBZ">uses</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">analogous</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">framework</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">signaling</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">IL-1</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NNS">phagocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@16-12-2010
1007587301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Detection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="VBN">phosphorylated</tok> <tok cat="NN">STAT-1</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">flow</tok> <tok cat="NN">cytometry</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
1007587302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">applied</tok> <cons cat="NP"><tok cat="NN">flow</tok> <tok cat="NN">cytometry</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">investigation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interferon-gamma</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1007587303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">approach</tok></cons> <cons cat="VP"><tok cat="VBZ">uses</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">monoclonal</tok> <tok cat="NNS">antibodies</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">distinguish</tok> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">native</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">phosphorylated</tok></cons></cons> <tok cat="NNS">forms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT-1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1007587304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">enables</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">rapid</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">quantitative</tok></cons></cons> <tok cat="NN">assessment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT-1</tok> <tok cat="NN">phosphorylation</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">discrete</tok> <tok cat="NN">cell</tok> <tok cat="NN">basis</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADJP"><tok cat="RBR">more</tok> <tok cat="JJ">sensitive</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJR">less</tok> <tok cat="NN">time</tok> <tok cat="JJ">consuming</tok></cons></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><tok cat="NN">immunoblotting</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1007587305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">allows</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">discrimination</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">mixture</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">differ</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">interferon-gamma</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1007587306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">approach</tok></cons> <cons cat="VP"><tok cat="MD">should</tok> <cons cat="VP"><tok cat="VB">allow</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">evaluation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">different</tok> <tok cat="JJ">intracellular</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">monoclonal</tok> <tok cat="NNS">reagents</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">specific</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">native</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="NN">activation</tok> <tok cat="JJ">modified</tok></cons></cons> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1007587307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Application</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">testing</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">should</tok> <cons cat="VP"><tok cat="VB">prove</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">valuable</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">screening</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">signaling</tok> <tok cat="NNS">defects</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">selected</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">recurrent</tok> <tok cat="NNS">infections</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1007587308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">technique</tok></cons> <cons cat="VP"><tok cat="MD">should</tok> <cons cat="VP"><tok cat="VB">permit</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">dissection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">full</tok> <tok cat="NN">range</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">protein</tok> <tok cat="NN">level</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1007715601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Glucocorticoid</tok> <tok cat="NN">hormone</tok> <tok cat="NN">suppression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">human</tok> <tok cat="JJ">neutrophil-mediated</tok></cons> <tok cat="NN">tumor</tok> <tok cat="NN">cell</tok> <tok cat="NN">cytostasis</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
1007715602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NNS">hormones</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">neutrophil-mediated</tok> <tok cat="NN">tumor</tok> <tok cat="NN">cell</tok> <tok cat="NN">cytostasis</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">hydrocortisone</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">synthetic</tok> <tok cat="NN">hormone</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">dexamethasone</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Dex</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="NN">cytostasis</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">presence</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">absence</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor-alpha</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
1007715603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Dex</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">completely</tok></cons> <tok cat="VBN">reversed</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok> <tok cat="NN">antagonist</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">RU38486</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1007715604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">clarify</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">underlying</tok> <tok cat="NNS">mechanisms</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i2" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="UCP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Dex</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok> <tok cat="NN">avidity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">beta2</tok> <tok cat="NN">integrin</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">neutrophil</tok> <tok cat="NN">surface</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><cons cat="WHADVP" id="i3"><tok cat="WRB">how</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="PP" adv="TMP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">turn</tok></cons></cons> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><tok cat="JJ">neutrophil-to-tumor</tok> <tok cat="NN">cell</tok> <tok cat="NN">binding</tok></cons><cons cat="ADVP" ref="i3" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1007715605@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="NN">Dex</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NN">neutrophil</tok> <tok cat="NNS">properties</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">RU38486</tok></cons> <cons cat="ADVP"><tok cat="RB">completely</tok></cons> <cons cat="VP"><tok cat="VBD">suppressed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">forms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Dex</tok> <tok cat="NN">inhibition</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1007715606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i5" id="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">Taken</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">together</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">hormone</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">neutrophil-mediated</tok> <tok cat="NN">tumor</tok> <tok cat="NN">cell</tok> <tok cat="NN">cytostasis</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="PP" role="PRD"><cons cat="ADVP"><cons cat="ADVP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok></cons> <tok cat="RB">partially</tok></cons> <tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">lowering</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ligand</tok> <tok cat="NN">binding</tok> <tok cat="NN">avidity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">beta2</tok> <tok cat="NN">integrin</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">neutrophil</tok> <tok cat="NN">surface</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
1007850201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">Abnormalities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cyclic</tok> <tok cat="NN">adenosine</tok> <tok cat="NN">monophosphate</tok> <tok cat="NN">signaling</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">platelets</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">untreated</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">bipolar</tok> <tok cat="NN">disorder</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1007850202@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">BACKGROUND</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NNS">Abnormalities</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">cyclic</tok> <tok cat="NN">adenosine</tok> <tok cat="NN">monophosphate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">cAMP</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="JJ">-dependent</tok></cons> <tok cat="NN">phosphorylation</tok> <tok cat="NN">system</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <tok cat="VBN">reported</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">bipolar</tok> <tok cat="NN">disorder</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@16-12-2010
1007850203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">evaluated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">immunoreactivity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">regulatory</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">catalytic</tok></cons></cons> <tok cat="NNS">subunits</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">cAMP-dependent</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">A</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">1</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NNS">substrates</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Rap1</tok></cons><tok cat="COMMA">,</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">platelets</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">untreated</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">euthymic</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">manic</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">depressed</tok></cons></cons> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">bipolar</tok> <tok cat="NN">disorder</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">subjects</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
1007850204@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">METHODS</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="NNS">Platelets</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">collected</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">112</tok> <tok cat="JJ">drug-free</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">bipolar</tok> <tok cat="NN">disorder</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">52</tok> <tok cat="JJ">euthymic</tok><cons cat="NP" null="QSTN"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">29</tok> <tok cat="JJ">depressed</tok><cons cat="NP" null="QSTN"></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">31</tok> <tok cat="JJ">manic</tok><cons cat="NP" null="QSTN"></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">62</tok> <tok cat="JJ">healthy</tok> <tok cat="NNS">subjects</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1007850205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">cAMP-dependent</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Rap1</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">assessed</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Western</tok> <tok cat="NN">blot</tok> <tok cat="NN">analysis</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">immunostaining</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">computer-assisted</tok> <tok cat="NN">imaging</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1007850206@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">RESULTS</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">immunolabeling</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">catalytic</tok> <tok cat="NN">subunit</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">cAMP-dependent</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">significantly</tok> <tok cat="JJ">different</tok></cons> <cons cat="PP"><tok cat="IN">among</tok> <cons cat="NP"><tok cat="NNS">groups</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons><cons cat="NP" role="PRD"><tok cat="JJR">&lt;</tok><tok cat="CD">.001</tok></cons></cons><tok cat="RRB">)</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJR">higher</tok> <tok cat="NNS">values</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">untreated</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">depressed</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">manic</tok></cons></cons> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">bipolar</tok> <tok cat="NN">disorder</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="VBN">compared</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">untreated</tok> <tok cat="JJ">euthymic</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">bipolar</tok> <tok cat="NN">disorder</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">subjects</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@16-12-2010
1007850207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="DT">No</tok> <tok cat="JJ">significant</tok> <tok cat="NNS">differences</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">immunolabeling</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">subunits</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">I</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">II</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">cAMP-dependent</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1007850208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">immunolabeling</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Rap1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="RB">significantly</tok> <tok cat="JJR">higher</tok></cons> <cons cat="PP" ref="i5" null="ICH"></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons><cons cat="NP" role="PRD"><tok cat="JJR">&lt;</tok><tok cat="CD">.001</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">untreated</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">euthymic</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">depressed</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">manic</tok></cons></cons> <tok cat="NNS">patients</tok></cons></cons> <cons cat="PP" id="i5"><tok cat="IN">than</tok> <cons cat="FRAG"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">persons</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1007850209@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">CONCLUSIONS</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NNS">Levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Rap1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">catalytic</tok> <tok cat="NN">subunit</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">cAMP-dependent</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">altered</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">platelets</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">bipolar</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1007850210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">clues</tok></cons> <cons cat="PP"><tok cat="IN">toward</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">understanding</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cAMP</tok> <tok cat="NN">signaling</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathogenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">bipolar</tok> <tok cat="NN">disorder</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1007910601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">Selective</tok> <tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="NN">significance</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p38alpha</tok> <tok cat="JJ">mitogen-activated</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">lipopolysaccharide-stimulated</tok> <tok cat="NNS">neutrophils</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1007910602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">leukocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">proinflammatory</tok> <tok cat="NNS">stimuli</tok></cons></cons></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">selectively</tok></cons> <tok cat="VBZ">initiates</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">sequential</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">kinases</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1007910603@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Lipopolysaccharide</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LPS</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">neutrophils</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">result</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">p38</tok> <tok cat="JJ">mitogen-activated</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MAPk</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <tok cat="RB">however</tok><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">upstream</tok> <tok cat="NN">activator</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p38</tok> <tok cat="NN">MAPk</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unknown</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">consequences</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p38</tok> <tok cat="NN">MAPk</tok> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">remain</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">largely</tok> <tok cat="JJ">undefined</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@16-12-2010
1007910604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">MAPk</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MKK</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="NN">p38</tok> <tok cat="NN">MAPk</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">LPS</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">p38</tok> <tok cat="NN">MAPk</tok> <tok cat="NNS">isoforms</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" id="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">part</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">functional</tok> <tok cat="NNS">responses</tok></cons> <cons cat="VP"><tok cat="VBN">affected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">p38</tok> <tok cat="NN">MAPk</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
1007910605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">MKK3</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">MKK4</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">MKK6</tok></cons></cons> <cons cat="ADVP"><tok cat="DT">all</tok></cons></cons> <cons cat="VP"><tok cat="VBD">activated</tok> <cons cat="NP"><tok cat="NN">p38</tok> <tok cat="NN">MAPk</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">experimental</tok> <tok cat="NNS">models</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="RB">only</tok> <tok cat="NN">MKK3</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">recombinant</tok> <tok cat="NN">p38</tok> <tok cat="NN">MAPk</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">LPS-treated</tok> <tok cat="NNS">neutrophils</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1007910606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" tpc="TPC" id="i6"><tok cat="IN">Of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">p38</tok> <tok cat="NN">MAPk</tok> <tok cat="NNS">isoforms</tok></cons> <cons cat="VP"><tok cat="VBN">studied</tok><cons cat="NP" null="NONE"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="RB">only</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p38alpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p38delta</tok></cons></cons></cons> <cons cat="PP" ref="i6" null="T"></cons></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">neutrophils</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1007910607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">LPS</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="ADVP"><tok cat="RB">selectively</tok></cons> <cons cat="VP"><tok cat="VBD">activated</tok> <cons cat="NP"><tok cat="NN">p38alpha</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@6@@oe@16-12-2010
1007910608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Specific</tok> <tok cat="NNS">inhibitors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p38alpha</tok> <tok cat="NN">MAPk</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">blocked</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">LPS-induced</tok> <tok cat="NN">adhesion</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons><tok cat="RRB">)</tok></cons> <tok cat="NN">activation</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">synthesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor-alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1007910609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p38alpha</tok> <tok cat="NN">MAPk</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">transient</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">accumulation</tok></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">persistent</tok> <tok cat="NN">loss</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">synthesis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1007910610@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">support</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">pathway</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i8"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">LPS</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">neutrophils</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">MKK3</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" id="i10" role="SBJ" null="T"></cons><cons cat="PP" adv="TMP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">turn</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="NN">p38alpha</tok> <tok cat="NN">MAPk</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="ADVP" adv="TMP"><tok cat="RB">ultimately</tok></cons> <cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">adhesion</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">enhanced</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">synthesis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><cons cat="PP" ref="i8" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
1008053201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">IL-4-inducible</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">I</tok> <tok cat="CC">and</tok> <tok cat="NN">type</tok> <tok cat="CD">II</tok> <tok cat="NNS">interferons</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1008053202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">Th2-type</tok> <tok cat="NNS">cytokines</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-4</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-4</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-13</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-13</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">distinct</tok> <tok cat="NN">subset</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">genes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">FcepsilonRIIb</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CD23</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">15-lipoxygenase</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="NN">receptor</tok> <tok cat="NN">antagonist</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-1ra</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">I</tok> <tok cat="CC">and</tok> <tok cat="NN">type</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">II</tok> <tok cat="NN">IL-1</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-1R</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@53@@oe@16-12-2010
1008053203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Type</tok> <tok cat="CD">I</tok> <tok cat="NNS">interferons</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IFN-alpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IFN-beta</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">II</tok> <tok cat="NN">interferon</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-4</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-13</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1008053204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i2"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">IFNs</tok></cons> <cons cat="VP"><tok cat="VBP">mediate</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP" ref="i2" null="T"></cons></cons></cons></cons></cons><cons cat="VP"><tok cat="VBZ">has</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">defined</tok><cons cat="NP" ref="i1" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1008053205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">overview</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">discuss</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">STAT6</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">transducer</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">activator</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription-6</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">mediating</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">IL-4-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">IL-13-induced</tok></cons></cons> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1008053206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VB">discuss</tok> <cons cat="NP"><tok cat="PRPP">our</tok> <tok cat="JJ">recent</tok> <tok cat="NNS">findings</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="NN">type</tok> <tok cat="CD">I</tok> <tok cat="CC">and</tok> <tok cat="NN">type</tok> <tok cat="CD">II</tok> <tok cat="NNS">IFNs</tok></cons> <cons cat="VP"><tok cat="VBP">suppress</tok> <cons cat="NP"><tok cat="JJ">IL-4/IL-13-inducible</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inhibiting</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">translocation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT6</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1008053207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">ability</tok> <cons cat="S" ref="i4" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">I</tok> <tok cat="CC">and</tok> <tok cat="NN">type</tok> <tok cat="CD">II</tok> <tok cat="NNS">IFNs</tok></cons></cons> <cons cat="S" id="i4"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><tok cat="JJ">IL-4/IL-13-induced</tok> <tok cat="NN">STAT6</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" syn="COOD" role="PRD"><cons cat="ADJP"><cons cat="NP"><tok cat="NN">dose-</tok></cons> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">time-dependent</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unique</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">because</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">IFNs</tok></cons> <cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">fibroblasts</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
1008053208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT6</tok> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">evident</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">unless</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">preincubated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IFN</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="QP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok> <tok cat="CD">1</tok></cons> <tok cat="NN">h</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">before</tok> <cons cat="NP"><tok cat="NN">IL-4</tok> <tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1008053209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><tok cat="NN">inhibition</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">actinomycin</tok> <tok cat="NN">D</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">requirement</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="FW">de</tok> <tok cat="FW">novo</tok></cons> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1008053210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">propose</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">model</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i8"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">IFN</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">de</tok> <tok cat="FW">novo</tok></cons> <tok cat="NN">synthesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NN">factor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="ADVP"><tok cat="RB">possibly</tok></cons> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">one</tok> <tok cat="CC">or</tok> <tok cat="JJR">more</tok></cons> <tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">SOCS/ SSI/CIS</tok> <tok cat="NN">gene</tok> <tok cat="NN">family</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="ADJP"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suppressing</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT6</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-4</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-13</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><cons cat="PP" ref="i8" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
1008053211@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Because</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">STAT6</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">essential</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">IL-4/IL-13-induced</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok> <cons cat="S" ref="i9" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IFN-beta</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons></cons> <cons cat="S" id="i9"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><tok cat="NN">STAT6</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">provides</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">explanation</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="SBAR"><cons cat="WHADVP" id="i10"><tok cat="WRB">how</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">IFNs</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">suppress</tok> <cons cat="NP"><tok cat="JJ">IL-4/IL-13-inducible</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons><cons cat="ADVP" ref="i10" null="T"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
1008054401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Lineage-specific</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT3</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">interferon-gamma</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">neutrophils</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
1008054402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interferon-gamma</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">heterodimeric</tok> <tok cat="NN">receptor</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">tyrosine</tok> <tok cat="NNS">kinases</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">JAK1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">JAK2</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBN">followed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT1alpha</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1008054403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="JJ">Selective</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT1alpha</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IFN-gamma</tok> <tok cat="NN">receptor</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">achieved</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">cytosolic</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">motif</tok></cons> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><tok cat="NN">Y440</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IFN-gamma</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha-chain</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">SH2</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT1alpha</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1008054404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">STAT1alpha</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i2" role="SBJ"><tok cat="NN">STAT3</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">neutrophils</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1008054405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT3</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">eosinophils</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HL-60</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">STAT3</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1008054406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">cell</tok> <tok cat="JJ">type-specific</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT3</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">neutrophils</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" id="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">differentiated</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">CD34+</tok> <tok cat="JJ">hematopoietic</tok> <tok cat="NN">stem</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1008054407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">a</tok> <tok cat="JJ">single</tok> <tok cat="NN">cytokine</tok> <tok cat="NN">receptor</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NN">STAT</tok> <tok cat="NN">family</tok> <tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">cell-specific</tok> <tok cat="NN">manner</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">result</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">cell-specific</tok> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1008087501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">p50/p65</tok> <tok cat="NN">heterodimer</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="JJ">pro-interleukin-1beta</tok> <tok cat="NN">gene</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">subregions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">upstream</tok> <tok cat="NN">enhancer</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1008087502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">between</tok><cons cat="NP"><cons cat="QP"><tok cat="CD">-3134</tok> <tok cat="CC">and</tok> <tok cat="CD">-2729</tok></cons> <tok cat="NN">bp</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">upstream</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">pro-interleukin</tok> <tok cat="NN">1beta</tok> <tok cat="LRB">(</tok><tok cat="NN">proIL-1beta</tok><tok cat="RRB">)</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">LPS-responsive</tok> <tok cat="NN">enhancer</tok> <tok cat="NN">element</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1008087503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">influence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-3134</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-2987</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">proIL-1beta</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">LPS-stimulated</tok> <tok cat="NN">Raw</tok> <tok cat="CD">264.7</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">detail</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1008087504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">transfection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">fos -CAT</tok></cons> <tok cat="NNS">constructs</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP"><tok cat="JJ">serial</tok> <tok cat="JJ">5'-deletion</tok> <tok cat="NNS">mutations</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-3134</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-3059</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="S"><cons cat="NP" ref="i4" id="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">LPS</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
1008087505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Gel</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok> <tok cat="NNS">studies</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">synthetic</tok> <tok cat="NNS">oligonucleotides</tok></cons> <cons cat="VP"><tok cat="VBG">corresponding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">partial</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">latter</tok> <tok cat="NN">region</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="VBN">stimulated</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">protein</tok> <tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-3110</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-3090</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-3079</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-3059</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
1008087506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="JJ">specific</tok> <tok cat="NNS">bindings</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">time</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">LPS</tok> <tok cat="NN">dose</tok></cons></cons> <tok cat="JJ">dependent</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
1008087507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">supershift</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NNS">antibodies</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">suggested</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">both</tok> <tok cat="VBG">binding</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NNS">components</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p50</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">contain</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p52</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">c-Rel</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Rel</tok> <tok cat="NN">B</tok></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Fos</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">C-Jun</tok></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CREB</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">C/EBPbeta</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-IL6</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@55@@oe@16-12-2010
1008087508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Mutation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">either</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">putative</tok> <tok cat="JJ">NF-kappaB-binding</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">enhancer</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">decreased</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">LPS-stimulated</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1008087509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBD">indicated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">NF-kappaB-binding</tok> <tok cat="NNS">sites</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-3134</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-3059</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">critical</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">proIL-1beta</tok> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1008087510@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="LST"><tok cat="NN">Copyright</tok></cons> <cons cat="NP" adv="TMP"><tok cat="CD">1999</tok></cons> <cons cat="NP"><tok cat="NNP">Academic</tok> <tok cat="NNP">Press</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@5@@oe@16-12-2010
1008090801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">modulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">myelopoiesis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">leukemogenesis</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1008090802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="JJ">Acute</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">leukemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AML</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">still</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">mortality</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="QP"><tok cat="CD">60</tok> <tok cat="TO">to</tok> <tok cat="CD">80</tok></cons><tok cat="NN">%</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1008090803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="NN">AML</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">characterized</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">block</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
1008090804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PU.1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">C/EBPalpha</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">responsible</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">stem</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NNS">granulocytes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1008090805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">particular</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PU.1</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">macrophage</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok> <tok cat="LRB">(</tok><tok cat="NN">M-CSF</tok><tok cat="RRB">)</tok> <tok cat="NN">receptor</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">development</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">C/EBPalpha</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">increases</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">granulocyte</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok> <tok cat="LRB">(</tok><tok cat="NN">G-CSF</tok><tok cat="RRB">)</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="NNS">granulocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
1008090806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">AML</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="JJ">chromosomal</tok> <tok cat="NNS">aberrations</tok></cons> <cons cat="VP"><tok cat="VBP">result</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">oncoproteins</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">AML1/ETO</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">PML/RARalpha</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">Ras</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">deregulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">genes</tok></cons> <cons cat="ADJP"><tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">myelopoiesis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1008090807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">AML1/ETO</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">C/EBPalpha</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><tok cat="JJ">C/EBPalpha-dependent</tok> <tok cat="NN">transcription</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">block</tok> <cons cat="NP"><tok cat="JJ">granulocytic</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1008090808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i4" role="SBJ"><tok cat="NN">AML1/ETO</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VB">synergize</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">AML1</tok></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">enhance</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">M-CSF</tok> <tok cat="NN">receptor</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1008090809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">On</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">other</tok> <tok cat="NN">hand</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">PML/RARalpha</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><cons cat="VP"><tok cat="VBZ">causes</tok> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">repression</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">recruiting</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">corepressor</tok> <tok cat="LRB">(</tok><tok cat="NN">N-CoR</tok><tok cat="RRB">)</tok> <tok cat="NN">histone</tok> <tok cat="NN">deacetylase</tok> <tok cat="NN">complex</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DNA</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">decreased</tok> <tok cat="NN">histone</tok> <tok cat="NN">acetylation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">repressive</tok> <tok cat="NN">chromatin</tok> <tok cat="NN">organization</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
1008090810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">describe</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">methods</tok></cons> <cons cat="WHADVP" id="i11" null="ZERO"></cons><cons cat="S"><cons cat="NP" id="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">investigate</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S" ref="i8" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="SBAR"><cons cat="WHADVP" id="i7"><tok cat="WRB">how</tok></cons> <cons cat="S" ref="i8" null="RNR"></cons></cons></cons><cons cat="S" id="i8"><cons cat="NP" role="SBJ"><tok cat="JJ">signaling</tok> <tok cat="NNS">agonists</tok></cons> <cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="ADVP" ref="i7" null="T"></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="SBAR"><cons cat="WHADVP" id="i9"><tok cat="WRB">how</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">oncoproteins</tok></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">cause</tok> <cons cat="NP"><tok cat="NN">AML</tok></cons> <cons cat="ADVP" ref="i9" null="T"></cons></cons></cons></cons></cons></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">modulating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">pivotal</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">development</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i11" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1008090811@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="LST"><tok cat="NN">Copyright</tok></cons> <cons cat="NP" adv="TMP"><tok cat="CD">1999</tok></cons> <cons cat="NP"><tok cat="NNP">Academic</tok> <tok cat="NNP">Press</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@5@@oe@16-12-2010
1008094801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TRAF2</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CD40</tok> <tok cat="JJ">cytoplasmic</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
1008094802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">TRAF2</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">signal</tok> <tok cat="NN">transducing</tok> <tok cat="NN">adaptor</tok> <tok cat="NN">molecule</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CD40</tok> <tok cat="JJ">cytoplasmic</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1008094803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="JJ">phosphorylated</tok><cons cat="NP" ref="i2" id="i3" null="NONE"></cons><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">predominantly</tok></cons> <tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">serine</tok> <tok cat="NNS">residues</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i5"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" id="i4" role="SBJ" null="NONE"></cons><cons cat="ADVP" adv="TMP"><tok cat="RB">transiently</tok></cons> <cons cat="VP"><tok cat="VBN">overexpressed</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="CD">293</tok> <tok cat="NNS">cells</tok></cons></cons><cons cat="ADVP" ref="i5" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1008094804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">related</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">events</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" id="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">TRAF2</tok></cons></cons> <cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">circumstances</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">since</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="CD">two</tok> <tok cat="JJ">nonfunctional</tok> <tok cat="NNS">mutants</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i9" id="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">phosphorylated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="ADVP"><cons cat="ADVP"><tok cat="RB">significantly</tok> <tok cat="RBR">less</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">wild-type</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1008094805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phosphorylation</tok> <tok cat="NN">status</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TRAF2</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="JJ">significant</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok> <cons cat="S" ref="i11" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="S" id="i11"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">CD40</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">evidenced</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="PRPP">our</tok> <tok cat="NNS">observations</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">CD40</tok> <tok cat="JJ">cytoplasmic</tok> <tok cat="JJ">domain</tok></cons> <cons cat="VP"><tok cat="VBD">interacted</tok> <cons cat="ADVP"><tok cat="RB">preferentially</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="VBN">underphosphorylated</tok> <tok cat="NN">TRAF2</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">phosphatase</tok> <tok cat="NN">treatment</tok></cons> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP"><tok cat="VBD">enhanced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TRAF2</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">CD40</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@49@@oe@16-12-2010
1008094806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">studies</tok></cons></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i13" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TRAF2</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">likely</tok> <cons cat="S"><cons cat="NP" ref="i13" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><tok cat="NN">signaling</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <tok cat="NN">virtue</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">influence</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">CD40-TRAF2</tok> <tok cat="NN">interaction</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
1008094807@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="LST"><tok cat="NN">Copyright</tok></cons> <cons cat="NP" adv="TMP"><tok cat="CD">1999</tok></cons> <cons cat="NP"><tok cat="NNP">Academic</tok> <tok cat="NNP">Press</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@5@@oe@16-12-2010
1008213401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">Cobalt</tok> <tok cat="JJ">chloride-induced</tok></cons> <tok cat="NN">signaling</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">endothelium</tok></cons> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">augmented</tok> <tok cat="NN">adherence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">sickle</tok> <tok cat="JJ">red</tok> <tok cat="NN">blood</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">transendothelial</tok> <tok cat="NN">migration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">monocyte-like</tok> <tok cat="NN">HL-60</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">PAF-receptor</tok> <tok cat="NN">antagonist</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1008213402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">hypoxia</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">sickle</tok> <tok cat="JJ">red</tok> <tok cat="NN">blood</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SS</tok> <tok cat="NN">RBC</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">leukocytes</tok></cons></cons> <cons cat="VP"><tok cat="VBP">exhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">adherence</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">vascular</tok> <tok cat="NN">endothelium</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">diapedesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">leukocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">blood</tok> <tok cat="NN">vessel</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">increases</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1008213403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons> <cons cat="VP"><tok cat="VBN">caused</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">hypoxia</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">poorly</tok></cons> <tok cat="VBN">understood</tok><cons cat="NP" ref="i2" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1008213404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">utilized</tok> <cons cat="NP"><tok cat="NN">CoCl2</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">mimetic</tok> <tok cat="NN">molecule</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">hypoxia</tok></cons></cons></cons></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">study</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1008213405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">umbilical</tok> <tok cat="NN">vein</tok> <tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HUVEC</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">CoCl2</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="CD">2</tok> <tok cat="NN">mM</tok></cons> <tok cat="NN">concentration</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">surface</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">subset</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">CAMs</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">VCAM-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">NF-kappaB</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HUVEC</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
1008213406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i11" role="SBJ"><tok cat="NN">CoCl2</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">time-dependent</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">mitogen-activated</tok> <tok cat="NN">protein</tok> <tok cat="LRB">(</tok><tok cat="NN">MAP</tok><tok cat="RRB">)</tok> <tok cat="NN">kinase</tok> <tok cat="NN">isoform</tok> <tok cat="NN">ERK2</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <tok cat="VBG">affecting</tok> <cons cat="NP"><tok cat="NN">ERK1</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><cons cat="COMP" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">ERK2</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">preferred</tok> <tok cat="NN">substrate</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">upstream</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">MAPK</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
1008213407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Inhibitors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">MAP</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PD98059</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">platelet-activating</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PAF</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="NN">- receptor</tok></cons> <tok cat="NN">antagonist</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CV3988</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">CoCl2-induced</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">VCAM-1</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1008213408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Augmented</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">VCAM-1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">led</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">SS</tok> <tok cat="NN">RBC</tok> <tok cat="NN">adhesion</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">inhibitable</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">VCAM-1</tok> <tok cat="NN">antibody</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1008213409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Additionally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">CoCl2</tok></cons> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="QP"><tok cat="CD">two-</tok> <tok cat="TO">to</tok> <tok cat="JJ">threefold</tok></cons> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">rate</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transendothelial</tok> <tok cat="NN">migration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">monocyte-like</tok> <tok cat="NN">HL-60</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">twentyfold</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">platelet</tok> <tok cat="JJ">endothelial</tok> <tok cat="NN">cell</tok> <tok cat="NN">adhesion</tok> <tok cat="NNS">molecules</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PECAM-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1008213410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">transendothelial</tok> <tok cat="NN">migration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">antibody</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">PECAM-1</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
1008213411@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="CC">Both</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PECAM-1</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transendothelial</tok> <tok cat="NN">migration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">CoCl2</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GF109203X</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">augmented</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">phosphatase</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Calyculin</tok> <tok cat="NN">A</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1008213412@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">CoCl2-induced</tok> <tok cat="JJ">cellular</tok> <tok cat="NNS">signals</tok></cons> <cons cat="VP"><tok cat="VBG">directing</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">VCAM-1</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">HUVEC</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">involve</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">downstream</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">MAP</tok> <tok cat="NN">kinase</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PECAM-1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">result</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PKC</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
1008213413@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP"><tok cat="NN">PAF-receptor</tok> <tok cat="NN">antagonist</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">CoCl2-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">hypoxia-induced</tok></cons></cons> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">adhesion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SS</tok> <tok cat="NN">RBC</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">PECAM-1</tok> <tok cat="NN">phosphorylation</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">concomitant</tok> <tok cat="JJ">transendothelial</tok> <tok cat="NN">migration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1008243601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Impaired</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">DQ2</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">DQ8-binding</tok></cons></cons> <tok cat="NN">HSV</tok> <tok cat="NN">VP16</tok> <tok cat="NN">peptide</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">DQA1*0501/DQB1*0302</tok> <tok cat="JJ">trans</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">heterodimer</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1008243602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">DQalpha</tok> <tok cat="CC">and</tok> <tok cat="NN">DQbeta</tok> <tok cat="JJ">trans</tok> <tok cat="JJ">heterodimeric</tok> <tok cat="NN">HLA-DQ</tok> <tok cat="NNS">molecules</tok></cons> <cons cat="VP"><tok cat="VBP">form</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">individuals</tok></cons> <cons cat="ADJP"><tok cat="JJ">heterozygous</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DQ2</tok> <tok cat="CC">and</tok> <tok cat="NN">DQ8</tok> <tok cat="NNS">specificities</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1008243603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="JJ">Unique</tok> <tok cat="NNS">functions</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">disease</tok> <tok cat="NNS">associations</tok></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><cons cat="VP"><tok cat="VBN">postulated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">such</tok> <tok cat="NNS">trans-dimers</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">different</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cis-encoded</tok> <tok cat="NN">DQ</tok> <tok cat="NNS">molecules</tok></cons> <cons cat="VP"><tok cat="VBN">encoded</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">corresponding</tok> <tok cat="NNS">haplotypes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1008243604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">analyzed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok> <cons cat="S" ref="i3" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">trans-dimer</tok></cons> <cons cat="VP"><tok cat="VBN">encoded</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">HLA-DQA1*0501/DQB1*0302</tok></cons></cons></cons></cons></cons> <cons cat="S" id="i3"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">peptide</tok> <tok cat="NN">antigen</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">interacts</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">DQ</tok> <tok cat="NNS">molecules</tok></cons> <cons cat="VP"><tok cat="VBN">encoded</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="JJ">parental</tok> <tok cat="NNS">haplotypes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1008243605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="ADJP"><tok cat="RB">Markedly</tok> <tok cat="JJ">impaired</tok></cons> <tok cat="NN">binding</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok><cons cat="NP" ref="i5" id="i6" null="NONE"></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="ADJP" role="PRD"><tok cat="JJ">consistent</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">use</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NN">anchor</tok> <tok cat="NNS">residues</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">DQ</tok> <tok cat="JJ">cis-dimer</tok> <tok cat="NN">availability</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">peptide</tok> <tok cat="NN">binding</tok> <tok cat="NNS">interactions</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1008247301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Molecular</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">neutrophil-endothelial</tok> <tok cat="NN">cell</tok> <tok cat="NN">adhesion</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">redox</tok> <tok cat="NN">imbalance</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
1008247302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Previous</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NNS">thiols</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappaB</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NN">cell</tok> <tok cat="NN">adhesion</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1008247303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">designed</tok> <cons cat="NP" ref="i1" id="i2" null="NONE"></cons><cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NN">cell</tok> <tok cat="NN">glutathione</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GSH</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">oxidized</tok> <tok cat="NN">glutathione</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GSSG</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">alter</tok> <cons cat="NP"><tok cat="NN">neutrophil</tok> <tok cat="NN">adhesivity</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">define</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">underlies</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">GSSG/GSH-induced</tok> <tok cat="NN">adhesion</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
1008247304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">umbilical</tok> <tok cat="NN">vein</tok> <tok cat="JJ">endothelial</tok> <tok cat="NN">cell</tok> <tok cat="LRB">(</tok><tok cat="NN">HUVEC</tok><tok cat="RRB">)</tok> <tok cat="NNS">monolayers</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="CD">6</tok> <tok cat="NNS">hours</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="CD">0.2</tok> <tok cat="NN">mmol/L</tok></cons> <tok cat="NN">diamide</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="CD">1</tok> <tok cat="NN">mmol/L</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">buthionine</tok> <tok cat="NN">sulfoximine</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">BSO</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">decreased</tok> <cons cat="NP"><tok cat="NN">GSH</tok> <tok cat="NNS">levels</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ratio</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GSSG</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">GSH</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">toxicity</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
1008247305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NN">redox</tok> <tok cat="NNS">changes</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">similar</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">anoxia/reoxygenation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
1008247306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="NN">Diamide</tok> <tok cat="CC">plus</tok> <tok cat="JJ">BSO-induced</tok> <tok cat="NN">thiol/disulfide</tok> <tok cat="NN">imbalance</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">biphasic</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">neutrophil</tok> <tok cat="NN">adhesion</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NNS">HUVECs</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="JJ">peak</tok> <tok cat="NNS">responses</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="CD">15</tok> <tok cat="NNS">minutes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">phase</tok> <tok cat="CD">1</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">240</tok> <tok cat="NNS">minutes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">phase</tok> <tok cat="CD">2</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1008247307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">N-Acetylcysteine</tok> <tok cat="NN">treatment</tok></cons> <cons cat="VP"><cons cat="VP"><tok cat="VBD">attenuated</tok> <cons cat="NP"><tok cat="NN">neutrophil</tok> <tok cat="NN">adhesion</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">phases</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">indicated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">GSH</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">adhesion</tok> <tok cat="NNS">responses</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1008247308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Interestingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">phase</tok> <tok cat="CD">1</tok> <tok cat="NN">adhesion</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">inversely</tok></cons> <tok cat="VBN">correlated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">GSH</tok> <tok cat="NNS">levels</tok></cons></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">GSSG/GSH</tok> <tok cat="NN">ratio</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="NN">phase</tok> <tok cat="CD">2</tok> <tok cat="NN">neutrophil</tok> <tok cat="NN">adhesion</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">positively</tok></cons> <tok cat="VBN">correlated</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">GSSG/GSH</tok> <tok cat="NN">ratio</tok></cons></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">GSH</tok> <tok cat="NNS">levels</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1008247309@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="JJ">Intercellular</tok> <tok cat="NN">adhesion</tok> <tok cat="NN">molecule-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">P-selectin-specific</tok> <tok cat="JJ">monoclonal</tok> <tok cat="NNS">antibodies</tok></cons></cons> <cons cat="VP"><tok cat="VBD">attenuated</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">increased</tok> <tok cat="NN">neutrophil</tok> <tok cat="NN">adhesion</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">phases</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">an</tok> <tok cat="JJ">anti-E-selectin</tok> <tok cat="JJ">monoclonal</tok> <tok cat="NN">antibody</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">attenuated</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phase</tok> <tok cat="CD">2</tok> <tok cat="NN">response</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1008247310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Pretreatment</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">actinomycin</tok> <tok cat="NN">D</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">cycloheximide</tok></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">competing</tok> <tok cat="NNS">ds-oligonucleotides</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappaB</tok> <tok cat="CC">or</tok> <tok cat="NN">activator</tok> <tok cat="NN">protein-1</tok> <tok cat="NN">cognate</tok> <tok cat="NN">DNA</tok> <tok cat="NNS">sequences</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><cons cat="VP"><cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <tok cat="VBD">attenuated</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phase</tok> <tok cat="CD">2</tok> <tok cat="NN">response</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">implicated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="FW">de</tok> <tok cat="FW">novo</tok></cons> <tok cat="NN">protein</tok> <tok cat="NN">synthesis</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
1008247311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Surface</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">intercellular</tok> <tok cat="NN">adhesion</tok> <tok cat="NN">molecule-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">P-selectin</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">E-selectin</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NNS">HUVECs</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">correlated</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phase</tok> <tok cat="CD">1</tok> <tok cat="CC">and</tok> <tok cat="CD">2</tok> <tok cat="NN">neutrophil</tok> <tok cat="NN">adhesion</tok> <tok cat="NNS">responses</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1008247312@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBZ">demonstrates</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NN">cell</tok> <tok cat="NN">GSSG/GSH</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">cause</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">transcription-independent</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">transcription-dependent</tok></cons></cons> <tok cat="NN">surface</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">different</tok> <tok cat="JJ">endothelial</tok> <tok cat="NN">cell</tok> <tok cat="NN">adhesion</tok> <tok cat="NNS">molecules</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">2-phase</tok> <tok cat="JJ">neutrophil-endothelial</tok> <tok cat="NN">adhesion</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
1008672501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">MDS1/EVI1</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">enhances</tok> <cons cat="NP"><tok cat="NN">TGF-beta1</tok> <tok cat="NN">signaling</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">strengthens</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">growth-inhibitory</tok> <tok cat="NN">effect</tok></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">leukemia-associated</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok> <tok cat="NN">AML1/MDS1/EVI1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">product</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">t(3;21)</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">abrogates</tok> <cons cat="NP"><tok cat="NN">growth-inhibition</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">TGF-beta1</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1008672502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">MDS1/EVI1</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="CD">3</tok> <tok cat="NN">band</tok> <tok cat="NN">q26</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">encodes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">zinc-finger</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">transcription</tok> <tok cat="NN">activator</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="RB">not</tok> <tok cat="VBN">detected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="JJ">hematopoietic</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">several</tok> <tok cat="JJ">normal</tok> <tok cat="NNS">tissues</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1008672503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="NN">MDS1/EVI1</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">inappropriately</tok></cons> <tok cat="VBN">activated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NNS">leukemias</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="VBG">following</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">chromosomal</tok> <tok cat="NNS">rearrangements</tok></cons> <cons cat="VP"><tok cat="VBG">involving</tok> <cons cat="NP"><tok cat="NN">band</tok> <tok cat="NN">3q26</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1008672504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">rearrangements</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">lead</tok> <tok cat="RB">either</tok> <cons cat="PP" syn="COOD" id="i20"><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">truncation</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">repressor</tok> <tok cat="NN">EVI1</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" id="i21" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">seen</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">t(3;3)(q21;q26)</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">inv(3)(q21q26)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="VP"><cons map="i20" cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">fusion</tok></cons><cons cat="SBAR" ref="i4" null="ICH"></cons></cons></cons><tok cat="COMMA">,</tok> <cons map="i21" cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">seen</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">t(3;21)(q26;q22)</tok></cons></cons></cons></cons></cons> <cons cat="SBAR" id="i4"><cons cat="WHNP" id="i6"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok> <tok cat="NN">AML1/MDS1/EVI1</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
1008672505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">domain</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">AML1</tok></cons> <cons cat="VP"><tok cat="VBN">fused</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">in-frame</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">entire</tok> <tok cat="NN">MDS1/EVI1</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">exclusion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">first</tok> <tok cat="CD">12</tok> <tok cat="NN">amino</tok> <tok cat="NNS">acids</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1008672506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">report</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">hematopoietic</tok> <tok cat="NN">precursor</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">32Dcl3</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">normal</tok> <tok cat="NN">protein</tok> <tok cat="NN">MDS1/EVI1</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok> <tok cat="NN">AML1/MDS1/EVI1</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">factors</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">control</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">differentiation</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">replication</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
1008672507@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">32Dcl3</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">IL-3-dependent</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">growth</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBP">differentiate</tok> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="NNS">granulocytes</tok></cons></cons> <cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i10"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" id="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">exposed</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">G-CSF</tok></cons></cons><cons cat="ADVP" ref="i10" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1008672508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="PRP">They</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">growth-inhibited</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">TGF-beta1</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@6@@oe@16-12-2010
1008672509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MDS1/EVI1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">granulocytic</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">G-CSF</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AML1/MDS1/EVI1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">blocks</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">death</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1008672510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">effect</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">similar</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <tok cat="VBN">described</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NNS">others</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">32Dcl3</tok> <tok cat="NNS">cells</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i12"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i12" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP"><tok cat="JJ">transgenic</tok> <tok cat="NN">Evil</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1008672511@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok> <tok cat="NN">AML1/MDS1/EVI1</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><cons cat="ADVP"><tok cat="RB">completely</tok></cons> <tok cat="VBZ">abrogates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">growth-inhibitory</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TGF-beta1</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">allows</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">32Dcl3</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">proliferate</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">normal</tok> <tok cat="NN">protein</tok> <tok cat="NN">MDS1/EVI1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">opposite</tok> <tok cat="NN">effect</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">strengthens</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">growth-inhibitory</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TGF-beta1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@52@@oe@16-12-2010
1008672512@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="S"><cons cat="NP" ref="i13" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">yeast</tok> <tok cat="JJ">two-hybrid</tok> <tok cat="NN">system</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i13" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">EVI1</tok></cons> <tok cat="LRB">(</tok><cons cat="VP"><tok cat="VBN">contained</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">entirety</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">MDS1/EVI1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">AML1/MDS1/EVI1</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">physically</tok></cons> <tok cat="VBZ">interacts</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">SMAD3</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i14"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i14" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">intracellular</tok> <tok cat="NN">mediator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TGF-beta1</tok> <tok cat="NN">signaling</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
1008672513@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">correlated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">G-CSF</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">TGF-beta1</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok> <cons cat="S" ref="i15" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="UCP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">normal</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">fusion</tok></cons></cons> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="S" id="i15"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP" ref="i16" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="VP"><tok cat="VB">repress</tok> <cons cat="NP" ref="i16" null="RNR"></cons></cons></cons><cons cat="NP" id="i16"><cons cat="NP"><tok cat="NNS">promoters</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i17"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" id="i18" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">directly</tok></cons> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP" ref="i17" null="T"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i18" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="NN">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">promoter</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
1008672514@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">propose</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">mutations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MDS1/EVI1</tok></cons></cons> <cons cat="PP" syn="COOD"><tok cat="CC">either</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">truncation</tok></cons> <cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">repressor</tok> <tok cat="NN">EVI1</tok></cons></cons></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">fusion</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">AML1</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">lead</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">altered</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">growth</tok> <tok cat="CC">and</tok> <tok cat="NN">differentiation</tok> <tok cat="NNS">factors</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i19"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i19" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">result</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">leukemic</tok> <tok cat="NN">transformation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
1008672515@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">different</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">ectopically</tok></cons> <tok cat="VBG">expressing</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">normal</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">fusion</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons></cons><cons cat="NP" id="i22"><tok cat="NN">protein</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">G-CSF</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TGF-beta1</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">depend</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">different</tok> <tok cat="NN">transactivation</tok> <tok cat="NNS">properties</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">divergent</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">downstream</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
1008718101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Interferon-alpha</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STATs1</tok><tok cat="COMMA">,</tok> <tok cat="CD">-3</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">growth</tok> <tok cat="NN">arrest</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">independent</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Lck</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">active</tok> <tok cat="JJ">mitogen-activated</tok> <tok cat="NN">kinase</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1008718102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Type</tok> <tok cat="CD">I</tok> <cons cat="NP"><tok cat="NNS">interferons</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">IFNs</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">family</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cytokines</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">antiviral</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">antiproliferative</tok></cons></cons> <tok cat="NNS">effects</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1008718103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Data</tok></cons> <cons cat="PP"><tok cat="VBG">regarding</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">processes</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i2"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">cytokines</tok></cons> <cons cat="VP"><tok cat="VBP">transduce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">signals</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">membrane</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons></cons> <cons cat="PP" ref="i2" null="T"></cons></cons></cons></cons></cons></cons></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBG">becoming</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">increasingly</tok> <tok cat="JJ">complex</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1008718104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="ADJP"><tok cat="JJS">most</tok> <tok cat="VBN">characterized</tok></cons> <tok cat="NN">pathway</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="ADJP"><tok cat="IN">via</tok> <tok cat="NN">JAK-STAT</tok></cons> <tok cat="NN">signaling</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
1008718105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Previous</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBD">established</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potential</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Src-family</tok> <tok cat="NN">kinase</tok> <tok cat="NN">Lck</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">JAK-STAT</tok></cons> <tok cat="NN">signaling</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1008718106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i3" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">designed</tok> <cons cat="NP" ref="i3" id="i4" null="NONE"></cons><cons cat="S"><cons cat="NP" ref="i4" id="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">analyze</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Lck</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">IFN-alpha</tok> <tok cat="NN">signaling</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Jurkat</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">JCam</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">Lck-defective</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">JCam/Lck</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">JCam</tok> <tok cat="NNS">cells</tok></cons> <cons cat="SBAR"><tok cat="IN">with</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="NN">Lck</tok></cons> <cons cat="VP"><tok cat="VBN">restored</tok><cons cat="NP" ref="i11" null="NONE"></cons></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
1008718107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IFN-alpha</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><tok cat="NN">MAPK</tok> <tok cat="NN">activity</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="NN">Lck</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1008718108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">STATs1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-3</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">effectively</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">phosphorylated</tok> <cons cat="NP" ref="i6" null="NONE"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i6" id="i12" null="NONE"></cons></cons></cons><cons cat="S" func="ADV"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="NP"><tok cat="NN">DNA</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">absence</tok> <tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Lck</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">IFN-alpha-treated</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1008718109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IFN-alpha</tok></cons> <cons cat="VP"><tok cat="VBZ">exerts</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">antiproliferative</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">all</tok> <tok cat="CD">three</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1008718110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Lck</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">active</tok> <tok cat="NN">MAPK</tok></cons></cons> <cons cat="VP"><tok cat="VBP">do</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">IFN-alpha-induced</tok> <tok cat="NN">growth</tok> <tok cat="NN">arrest</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">induction</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT1s1</tok> <tok cat="CC">and</tok> <tok cat="CD">-3</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">ability</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1008718111@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="LST"><tok cat="NN">Copyright</tok></cons> <cons cat="NP" adv="TMP"><tok cat="CD">1999</tok></cons> <cons cat="NP"><tok cat="NNP">Academic</tok> <tok cat="NNP">Press</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@5@@oe@16-12-2010
1008718501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="VBN">Decreased</tok> <tok cat="JJ">proteasome-mediated</tok> <tok cat="NN">degradation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">elderly</tok></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">immune</tok> <tok cat="NN">senescence</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@16-12-2010
1008718502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFkappaB</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">highly</tok> <tok cat="VBN">regulated</tok></cons> <tok cat="NN">process</tok></cons> <cons cat="VP"><tok cat="VBG">requiring</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">ubiquitination</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">proteasome-mediated</tok></cons> <tok cat="NN">degradation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cytosolic</tok> <tok cat="NN">inhibitor</tok> <tok cat="NN">IkappaBalpha</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1008718503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Analyses</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IkappaBalpha</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">TNF-alpha-treated</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">young</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">elderly</tok></cons></cons> <tok cat="NNS">donors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">severely</tok> <tok cat="VBN">compromised</tok></cons> <tok cat="NN">degradation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IkappaBalpha</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">elderly</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1008718504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Examination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">activation-induced</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">ubiquitination</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IkappaBalpha</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">demonstrate</tok> <cons cat="NP"><tok cat="DT">any</tok> <tok cat="JJ">significant</tok> <tok cat="JJ">age-related</tok> <tok cat="NNS">alterations</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1008718505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">examination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">proteasome</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="JJ">fluorogenic</tok> <tok cat="NN">peptide</tok> <tok cat="NNS">assays</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">significant</tok> <tok cat="JJ">age-related</tok> <tok cat="NN">decline</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">chymotryptic</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1008718506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">decline</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">proteasome</tok> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">failure</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">fully</tok></cons> <tok cat="VB">degrade</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IkappaBalpha</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">elderly</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1008718507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">failure</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">degrade</tok> <cons cat="NP"><tok cat="NN">IkappaBalpha</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">underlie</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">observed</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">NFkappaB</tok> <tok cat="NN">induction</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">receptor</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">TNF-treated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">aging</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1008718508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="VBN">decreased</tok> <tok cat="JJ">proteasome-mediated</tok> <tok cat="NN">degradation</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">central</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">immune</tok> <tok cat="NN">dysfunction</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">accompanies</tok> <cons cat="NP"><tok cat="NN">aging</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1008718509@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="LST"><tok cat="NN">Copyright</tok></cons> <cons cat="NP" adv="TMP"><tok cat="CD">1999</tok></cons> <cons cat="NP"><tok cat="NNP">Academic</tok> <tok cat="NNP">Press</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@5@@oe@16-12-2010
1008764801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">T</tok> <tok cat="NN">helper</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="VP"><tok cat="VBZ">proceeds</tok> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">Stat1-dependent</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">Stat4-dependent</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">Stat4-independent</tok></cons></cons> <tok cat="NNS">phases</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1008764802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Much</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">our</tok> <tok cat="NN">focus</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">understanding</tok> <cons cat="NP"><tok cat="NN">Th1/Th2</tok> <tok cat="NN">development</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="PP" role="PRD"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">signals</tok></cons> <cons cat="VP"><tok cat="VBN">delivered</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-12</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">final</tok> <tok cat="NNS">determinants</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">terminal</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1008764803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Because</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">extinction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-12</tok> <tok cat="NN">signaling</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">Th2</tok> <tok cat="NN">development</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="ADVP"><tok cat="RB">potentially</tok></cons> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">imprinting</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RBR">more</tok> <tok cat="JJ">permanent</tok></cons> <tok cat="NN">Th2</tok> <tok cat="NN">phenotype</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">population</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NNS">parameters</tok></cons> <cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-12</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
1008764804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Whereas</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="NN">IL-4</tok></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">repress</tok> <cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="NN">IL-12</tok> <tok cat="NN">signaling</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-12R</tok> <tok cat="NN">beta</tok> <tok cat="CD">2</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i2" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mouse</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IFN-alpha</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">appear</tok> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><tok cat="NN">IL-12R</tok> <tok cat="NN">beta</tok> <tok cat="CD">2</tok> <tok cat="NN">expression</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">promote</tok> <cons cat="NP"><tok cat="NN">IL-12</tok> <tok cat="NN">responsiveness</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
1008764805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">propose</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="NN">Th1</tok> <tok cat="NN">development</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">considered</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">stages</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">capacitance</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">development</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1008764806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Capacitance</tok></cons> <cons cat="VP"><tok cat="MD">would</tok> <cons cat="ADVP"><tok cat="RB">simply</tok></cons> <cons cat="VP"><tok cat="VB">involve</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-12R</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">beta</tok> <tok cat="CD">1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">beta</tok> <tok cat="CD">2</tok></cons></cons> <tok cat="NNS">subunits</tok></cons><cons cat="PRN"><tok cat="COMMA">,</tok> <cons cat="FRAG"><cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">TCR</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="NP"><tok cat="NN">IL-4</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">IFNs</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1008764807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" tpc="TPC" id="i4"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">second</tok> <tok cat="NN">stage</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">development</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">propose</tok> <cons cat="SBAR" null="ZERO"></cons><cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">true</tok> <tok cat="NN">IL-12</tok> <tok cat="JJ">induced</tok> <tok cat="JJ">developmental</tok> <tok cat="NN">stage</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">involving</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Stat4</tok> <tok cat="JJ">inducible</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1008764808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">this</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VB">occur</tok> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="NN">IFN-alpha</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="NN">Stat4</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1008764809@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRP">It</tok></cons> <cons cat="SBAR" ref="i6" null="EXP"></cons></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">perhaps</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">possible</tok> <cons cat="SBAR" id="i6"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="DT">all</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Stat4</tok> <tok cat="NNS">actions</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">Th1</tok> <tok cat="NN">development</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">exert</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">directly</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">Stat4</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IFN-gamma</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="ADVP"><tok cat="RB">however</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="ADVP"><tok cat="RBR">more</tok> <tok cat="JJ">likely</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><tok cat="NN">Stat4</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">act</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><tok cat="NN">Th1</tok> <tok cat="NN">development</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <tok cat="JJ">non-cytokine</tok> <tok cat="NNS">genes</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WPP">whose</tok> <tok cat="JJ">stable</tok> <tok cat="NN">expression</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">maintains</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">state</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">Th1</tok> <tok cat="NN">cell</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@58@@oe@16-12-2010
1008799301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Identification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">upstream</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">elements</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">repress</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="JJ">beta-like</tok> <tok cat="NN">globin</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">primitive</tok> <tok cat="JJ">erythroid</tok> <tok cat="NN">environment</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1008799302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">investigations</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">focused</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">localizing</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cis-elements</tok></cons> <cons cat="ADJP"><tok cat="JJ">responsible</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">down</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">adult</tok> <tok cat="JJ">beta-like</tok> <tok cat="NN">globin</tok> <tok cat="NNS">genes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">delta</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">beta</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">immature</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">primitive</tok></cons></cons> <tok cat="JJ">erythroid</tok> <tok cat="NNS">tissues</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
1008799303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">studied</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transfection</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">K562</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">erythroleukemia</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">embryonic-fetal</tok> <tok cat="NN">phenotype</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1008799304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="VBN">Analyzed</tok> <tok cat="NN">DNA</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">included</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">delta</tok> <tok cat="CC">and</tok> <tok cat="NN">beta</tok> <tok cat="JJ">5'</tok> <tok cat="NN">flanking</tok> <tok cat="NNS">regions</tok></cons> <cons cat="VP"><tok cat="VBG">extending</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="QP"><tok cat="RB">approximately</tok> <tok cat="CD">-500</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">+50bp</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NNS">regions</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="JJ">truncated</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">delta</tok> <tok cat="CC">and</tok> <tok cat="NN">beta</tok> <tok cat="JJ">5'</tok> <tok cat="NN">flanking</tok> <tok cat="NNS">regions</tok></cons> <cons cat="VP"><tok cat="VBG">extending</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="QP"><tok cat="RB">approximately</tok> <tok cat="CD">-250</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="CD">+50</tok> <tok cat="NN">bp</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">chimeric</tok> <tok cat="NN">promoter</tok> <tok cat="NNS">constructions</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">consisted</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">distal</tok> <tok cat="NN">delta</tok> <tok cat="CC">or</tok> <tok cat="NN">beta</tok> <tok cat="NN">fragment</tok></cons> <cons cat="VP"><tok cat="VBN">fused</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">proximal</tok> <tok cat="NN">beta</tok> <tok cat="CC">or</tok> <tok cat="NN">delta</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@59@@oe@16-12-2010
1008799305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">CAT</tok> <tok cat="NN">reporter</tok> <tok cat="NNS">constructions</tok></cons></cons> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="JJ">appreciable</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CAT</tok> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">supported</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP" id="i5"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">beta</tok> <tok cat="NN">globin</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons map="i4" cat="NP" role="SBJ"><tok cat="RB">only</tok> <cons cat="ADJP"><tok cat="JJ">low</tok> <tok cat="NN">level</tok></cons> <tok cat="NN">activity</tok></cons> <cons map="i5" cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">delta</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1008799306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Truncation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">beta</tok> <tok cat="NN">globin</tok> <tok cat="NN">promoter</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">led</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="CD">2-3</tok> <tok cat="JJ">fold</tok></cons> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1008799307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">deletion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">upstream</tok> <tok cat="NN">portion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">delta</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">led</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="CD">10</tok> <tok cat="JJ">fold</tok></cons> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">expression</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1008799308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Coupling</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">upstream</tok> <tok cat="NN">beta</tok> <tok cat="NN">globin</tok> <tok cat="NN">sequence</tok></cons> <cons cat="NP"><cons cat="QP"><tok cat="IN">from</tok> <tok cat="RB">approximately</tok> <tok cat="CD">-500</tok> <tok cat="TO">to</tok> <tok cat="CD">-250</tok></cons> <tok cat="NN">bp</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">truncated</tok> <tok cat="NN">delta</tok> <tok cat="NN">promoter</tok> <tok cat="NN">fragment</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">led</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">complete</tok> <tok cat="NN">extinction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="ADJP" role="PRD"><tok cat="JJ">consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">negative</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">beta</tok> <tok cat="NN">globin</tok> <tok cat="NN">gene</tok> <tok cat="JJ">upstream</tok> <tok cat="NN">element</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
1008799309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Fusion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">upstream</tok> <tok cat="NN">portion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">delta</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">truncated</tok> <tok cat="NN">beta</tok> <tok cat="NN">globin</tok> <tok cat="NN">promoter</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">yielded</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">modest</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">strength</tok></cons></cons> <cons cat="PP"><tok cat="JJ">relative</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">truncated</tok> <tok cat="NN">beta</tok> <tok cat="NN">gene</tok> <tok cat="NN">promoter</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">positive</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">element</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">upstream</tok> <tok cat="NN">delta</tok> <tok cat="NN">globin</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@49@@oe@16-12-2010
1008799310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Site-directed</tok> <tok cat="NN">mutagenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">binding</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">repressor</tok> <tok cat="NNS">proteins</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">BP1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">BP2</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">upstream</tok> <tok cat="NN">portion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">beta</tok> <tok cat="NN">globin</tok> <tok cat="NN">gene</tok> <tok cat="NN">flanking</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">led</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="CD">4-6</tok> <tok cat="JJ">fold</tok></cons> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1008799311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">DNase</tok> <tok cat="CD">I</tok> <tok cat="NN">footprinting</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">upstream</tok> <tok cat="JJ">delta-globin</tok> <tok cat="NN">region</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">protected</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="VP"><tok cat="VBG">corresponding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">consensus</tok> <tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">GATA-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">BP2</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1008799312@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">confirm</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">sequences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">upstream</tok> <tok cat="NN">promoter</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">adult</tok> <tok cat="NN">beta</tok> <tok cat="NN">globin</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">factor-mediated</tok> <tok cat="NN">suppression</tok></cons></cons> <cons cat="PP" adv="TMP"><cons cat="ADVP"><tok cat="JJ">early</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">development</tok></cons></cons></cons> <tok cat="CC">and</tok> <tok cat="RB">then</tok> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">modulate</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">later</tok> <tok cat="NN">stage</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1008914001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Reduction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tumour</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">alpha</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">expression</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">signalling</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">thalassaemia</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">sickle</tok> <tok cat="NN">cell</tok> <tok cat="NN">anaemia</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">desferrioxamine</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1008914002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Recent</tok> <tok cat="NN">evidence</tok></cons> <cons cat="VP"><tok cat="VBZ">indicates</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">rate</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">progression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">disease</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <tok cat="VBN">reduced</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">thalassaemia</tok> <tok cat="JJ">major</tok> <tok cat="NNS">patients</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">doses</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">desferrioxamine</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">DFX</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1008914003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">authors</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">exposure</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">DFX</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">decreases</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">bioavailability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">tumour</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">stimulatory</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">replication</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
1008914004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">bioavailability</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">10</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">thalassaemia</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">sickle</tok> <tok cat="NN">cell</tok> <tok cat="NN">anaemia</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">given</tok> <cons cat="NP" null="NONE"></cons><cons cat="NP"><tok cat="NN">DFX</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="SBAR"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="CD">10</tok> <tok cat="JJ">untreated</tok> <tok cat="NNS">subjects</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">evaluated</tok><cons cat="NP" ref="i3" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1008914005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">Evidence</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">presented</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">showing</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="NN">DFX</tok> <tok cat="NN">treatment</tok></cons> <cons cat="VP"><tok cat="VBZ">reduces</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">bioavailability</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons><cons cat="NP" role="PRD"><tok cat="JJR">&lt;</tok><tok cat="CD">0.05</tok></cons></cons><tok cat="RRB">)</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inhibiting</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">steady</tok> <tok cat="NN">state</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons><cons cat="NP" role="PRD"><tok cat="JJR">&lt;</tok><tok cat="CD">0.05</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">enhancing</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">inactivation</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">soluble</tok> <tok cat="NN">TNF-alpha</tok> <tok cat="NN">receptor</tok> <tok cat="NN">type</tok> <tok cat="CD">II</tok></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons><cons cat="NP" role="PRD"><tok cat="JJR">&lt;</tok><tok cat="CD">0.05</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
1008914006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">DFX</tok> <tok cat="NN">treatment</tok></cons> <cons cat="VP"><tok cat="VBZ">limits</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">signalling</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons><cons cat="NP" role="PRD"><tok cat="JJR">&lt;</tok><tok cat="CD">0.005</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1008914007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="NN">TNF-alpha</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">bioavailability</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">signalling</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="JJ">impaired</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">patients</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">upon</tok> <cons cat="NP"><tok cat="NN">DFX</tok> <tok cat="NN">treatment</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1008914008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">delayed</tok> <tok cat="NN">progression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">infection</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1008914009@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="LST"><tok cat="NN">Copyright</tok></cons> <cons cat="NP" adv="TMP"><tok cat="CD">1999</tok></cons> <cons cat="NP"><tok cat="NNP">Academic</tok> <tok cat="NNP">Press</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@5@@oe@16-12-2010
1008956601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">adenylate</tok> <tok cat="NN">cyclase</tok> <tok cat="CC">and</tok> <tok cat="NN">p70(S6)-kinase</tok> <tok cat="NN">activation</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-10</tok> <tok cat="NN">up-regulation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gp41</tok> <tok cat="NN">envelope</tok> <tok cat="NN">protein</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1008956602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="JJ">previous</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">recombinant</tok> <tok cat="NN">gp41</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">aa565-647</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">extracellular</tok> <tok cat="NN">domain</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="JJ">transmembrane</tok> <tok cat="NN">glycoprotein</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-10</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-10</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1008956603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">signal</tok> <tok cat="NN">cascade</tok></cons> <cons cat="VP"><tok cat="VBG">transducing</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">effect</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">yet</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">clear</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
1008956604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="ADJP"><tok cat="JJ">gp41-induced</tok></cons> <tok cat="NN">IL-10</tok> <tok cat="NN">up-regulation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">previously</tok> <tok cat="VBN">described</tok></cons> <tok cat="JJ">synergistic</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cAMP</tok> <tok cat="CC">and</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NNS">pathways</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1008956605@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">gp41</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cAMP</tok> <tok cat="NN">accumulation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">time-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">concentration-dependent</tok></cons></cons> <tok cat="NN">manner</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">adenylate</tok> <tok cat="NN">cyclase</tok> <tok cat="NN">inhibitor</tok> <tok cat="NN">SQ</tok> <tok cat="CD">22536</tok></cons> <cons cat="VP"><tok cat="VBD">suppressed</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">gp41-induced</tok></cons> <tok cat="NN">IL-10</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1008956606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i2" role="SBJ"><tok cat="NN">gp41</tok></cons> <cons cat="VP"><tok cat="VBD">failed</tok> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">stimulate</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="SBAR"><tok cat="IN">in</tok> <tok cat="RB">as</tok> <tok cat="JJ">much</tok> <tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">no</tok> <tok cat="NN">NF-kappaB</tok></cons> <cons cat="VP"><tok cat="VBD">bound</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">main</tok> <tok cat="JJ">NF-kappaB-binding</tok> <tok cat="NN">site</tok> <tok cat="CD">2</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-10</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">gp41</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1008956607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok> <tok cat="NNS">pathways</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
1008956608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Specific</tok> <tok cat="NNS">inhibitors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">p70(S6)-kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">rapamycin</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Gi</tok> <tok cat="NN">protein</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">pertussis</tok> <tok cat="NN">toxin</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">prevented</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-10</tok> <tok cat="NN">production</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gp41</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">inhibitors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phosphatidylinositol</tok> <tok cat="NN">3-kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PI</tok> <tok cat="NN">3-kinase</tok></cons><tok cat="RRB">)</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">wortmannin</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">mitogen-activated</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MAPK</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">pathway</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PD</tok> <tok cat="CD">98059</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok><cons cat="VP" null="QSTN"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@54@@oe@16-12-2010
1008956609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons> <cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="NN">HIV-1</tok> <cons cat="ADJP"><tok cat="JJ">gp41-induced</tok></cons> <tok cat="NN">IL-10</tok> <tok cat="NN">up-regulation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="MD">may</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">involve</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok><tok cat="COMMA">,</tok> <tok cat="NN">MAPK</tok><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <tok cat="NN">PI</tok> <tok cat="NN">3-kinase</tok> <tok cat="NN">activation</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">rather</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">operate</tok> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">adenylate</tok> <tok cat="NN">cyclase</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">pertussis-toxin-sensitive</tok> <tok cat="NN">Gi/Go</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">effect</tok> <cons cat="NP"><tok cat="NN">p70(S6)-kinase</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
1008990701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Estrone</tok></cons> <cons cat="VP"><tok cat="VBZ">potentiates</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NN">cell</tok> <tok cat="NN">differentiation</tok></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="CD">17</tok> <tok cat="NN">beta-hydroxysteroid</tok> <tok cat="NN">dehydrogenase</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">modulating</tok> <cons cat="NP"><tok cat="NN">hemopoiesis</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@16-12-2010
1008990702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Hormones</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="CD">1</tok> <tok cat="NN">alpha, 25-dihydroxy</tok> <tok cat="NN">vitamin</tok> <tok cat="NN">D3</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">D3</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">all-trans</tok> <tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">9-cis</tok> <tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">stimulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NN">progenitor</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">hormone</tok> <tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1008990703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">sensitivity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">agents</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="ADVP"><tok cat="RB">merely</tok></cons> <cons cat="VP"><tok cat="VBN">governed</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NNS">receptors</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">availability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">ligand</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2" null="ZERO"></cons><cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="NP"><tok cat="PRP">them</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1008990704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Recent</tok> <tok cat="NNS">studies</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="PRPP">our</tok> <tok cat="NN">group</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">suggested</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">actions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">D3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">retinoids</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">myelopoiesis</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">influenced</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">endogenous</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="VP"><tok cat="VBG">involving</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NN">steroid</tok> <tok cat="NNS">hormones</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1008990705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">influence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">local</tok> <tok cat="NN">estrogen</tok> <tok cat="NN">metabolism</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HL60</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="JJ">primitive</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">progenitor</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1008990706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Quantitative</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">thin-layer</tok> <tok cat="NN">chromatography</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TLC</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">analyses</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="UCP" syn="COOD"><cons cat="NP"><tok cat="NN">HL60</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">normal</tok></cons></cons> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">generate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">estrone</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">E1</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">estradiol</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">E2</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1008990707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">Neither</tok> <tok cat="NN">cell</tok> <tok cat="NN">population</tok></cons> <cons cat="VP"><tok cat="VBD">generated</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">significant</tok> <tok cat="NNS">amounts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">E2</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">E1</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
1008990708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Reverse</tok> <tok cat="NN">transcriptase</tok> <tok cat="NN">polymerase</tok> <tok cat="NN">chain</tok> <tok cat="NN">reaction</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Northern</tok> <tok cat="NNS">analyses</tok></cons></cons> <cons cat="VP"><tok cat="VBD">confirmed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="UCP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">normal</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">leukemic</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">progenitor</tok></cons></cons> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">mRNA</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">type</tok> <tok cat="CD">I</tok> <tok cat="CC">and</tok> <tok cat="CD">IV</tok> <tok cat="NNS">isoforms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">17</tok> <tok cat="NN">beta-hydroxysteroid</tok> <tok cat="NN">dehydrogenase</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1008990709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">Conversion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">E2</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">E1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">upregulated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="CD">24</tok> <tok cat="NNS">hours</tok></cons></cons> <cons cat="SBAR" adv="TMP" role="PRD"><cons cat="WHADVP" id="i6"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="NN">HL60</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="JJ">all-trans</tok> <tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">D3</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">doses</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">toward</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">neutrophils</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i6" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1008990710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Similarly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="JJ">D3-induced</tok> <tok cat="NN">monocyte</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">progenitor</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">capacity</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">generate</tok> <cons cat="NP"><tok cat="NN">E1</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">E2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1008990711@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i10"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" syn="COOD" id="i11" role="SBJ"><cons cat="NP"><tok cat="NN">HL60</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">progenitor</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">exposed</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">exogenous</tok> <tok cat="NN">E1</tok></cons></cons> <cons cat="ADVP" ref="i10" null="T"></cons></cons></cons></cons></cons><cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBD">became</tok> <cons cat="ADJP" role="PRD"><tok cat="RBR">more</tok> <tok cat="JJ">sensitive</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">differentiation-inducing</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">D3</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1008990712@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Data</tok></cons> <cons cat="VP"><tok cat="VBN">presented</tok> <cons cat="NP" null="NONE"></cons></cons></cons><cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">further</tok> <tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">local</tok> <tok cat="NN">modulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">myelopoiesis</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">intracrine</tok> <tok cat="NNS">mechanisms</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1008990713@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">particular</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">local</tok> <tok cat="NN">metabolism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">steroids</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">normal</tok></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="ADJP"><tok cat="JJ">leukemic</tok> <tok cat="JJ">myeloid</tok></cons></cons> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">influences</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">responsiveness</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">D3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">retinoids</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1009033801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">relationship</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Ca2+-ATPase</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">freely</tok> <tok cat="JJ">exchangeable</tok></cons> <tok cat="NN">Ca2+</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">dense</tok> <tok cat="NNS">tubules</tok></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">study</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">platelets</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NNS">women</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@16-12-2010
1009033802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">main</tok> <tok cat="NNS">aims</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">work</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="S" role="PRD"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">examine</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">women</tok></cons></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">relationship</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">freely</tok> <tok cat="JJ">exchangeable</tok></cons> <tok cat="NN">Ca2+</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">FECa2+</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">dense</tok> <tok cat="NNS">tubules</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">sarco</tok><tok cat="LRB">(</tok><tok cat="NN">endo</tok><tok cat="RRB">)</tok><tok cat="JJ">plasmic</tok> <tok cat="NN">reticulum</tok> <tok cat="LRB">(</tok><tok cat="NN">SER</tok><tok cat="RRB">)</tok> <tok cat="NN">Ca2+-ATPase</tok> <tok cat="LRB">(</tok><tok cat="NN">SERCA</tok><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">platelets</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">relationship</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">parameters</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">blood</tok> <tok cat="NN">pressure</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">serum</tok> <tok cat="NNS">lipoproteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@60@@oe@16-12-2010
1009033803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NNS">Platelets</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">14</tok> <tok cat="JJ">white</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">13</tok> <tok cat="JJ">black</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons></cons><cons cat="NP" id="i2"><tok cat="NNS">women</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">good</tok> <tok cat="NN">health</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">studied</tok><cons cat="NP" ref="i1" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1009033804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">FECa2+</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">measured</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">ionomycin-evoked</tok> <tok cat="NN">Ca2+</tok> <tok cat="NN">release</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">presence</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">thapsigargin</tok></cons></cons><tok cat="RRB">)</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">Ca2+-free</tok> <tok cat="NN">medium</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1009033805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="NN">SERCA</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">measured</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">thapsigargin</tok> <tok cat="JJ">sensitive</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="NN">Ca2+</tok> <tok cat="JJ">dependent</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="NN">ouabain</tok> <tok cat="JJ">resistant</tok></cons><tok cat="COMMA">,</tok></cons> <tok cat="NN">ATP</tok> <tok cat="NNS">hydrolyses</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">platelet</tok> <tok cat="NNS">membranes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1009033806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="JJ">Relative</tok> <tok cat="NNS">expressions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">SERCA</tok> <tok cat="CD">2</tok> <tok cat="CC">and</tok> <tok cat="CD">3</tok> <tok cat="NNS">isoforms</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">Ras-related</tok> <tok cat="NN">protein</tok> <tok cat="LRB">(</tok><tok cat="NN">Rap</tok><tok cat="RRB">)</tok> <tok cat="CD">1</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">platelet</tok> <tok cat="NNS">membranes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">determined</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">Western</tok> <tok cat="NNS">immunoblots</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1009033807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="ADJP"><tok cat="RB">Highly</tok> <tok cat="JJ">significant</tok></cons> <tok cat="NNS">correlations</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">FECa2+</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">dense</tok> <tok cat="NNS">tubules</tok></cons> <cons cat="PP"><tok cat="IN">with</tok><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="LST"><tok cat="LS">1</tok><tok cat="RRB">)</tok></cons> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">maximal</tok> <tok cat="NN">reaction</tok> <tok cat="NN">velocity</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Vmax</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">SERCA</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">r</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">0.592</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">.0014</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="LST"><tok cat="LS">2</tok><tok cat="RRB">)</tok></cons> <cons cat="NP"><tok cat="NN">Rapl</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">r</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">0.551</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">.0035</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@49@@oe@16-12-2010
1009033808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i7" role="SBJ"><tok cat="JJ">negative</tok> <tok cat="NNS">correlations</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">FECa2+</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">dense</tok> <tok cat="NNS">tubules</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">age</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1009033809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">No</tok> <tok cat="NNS">correlations</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">variables</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">blood</tok> <tok cat="NN">pressure</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">serum</tok> <tok cat="NNS">lipoproteins</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1009033810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><cons cat="COMP" null="ZERO"></cons><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">FECa2+</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Vmax</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">SERCA</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">reliable</tok> <tok cat="NNS">indicators</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Ca2+</tok> <tok cat="NN">load</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">platelets</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NNS">women</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1009033811@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">women</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">unlike</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">previous</tok> <tok cat="NNS">observations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">men</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><tok cat="DT">these</tok> <tok cat="NN">platelet</tok> <tok cat="NNS">parameters</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">correlated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">blood</tok> <tok cat="NN">pressure</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">serum</tok> <tok cat="NNS">lipoproteins</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1009092301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Nonimmunoglobulin</tok> <tok cat="NN">gene</tok> <tok cat="NN">hypermutation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">germinal</tok> <tok cat="NN">center</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
1009092302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Somatic</tok> <tok cat="NN">hypermutation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RBS">most</tok> <tok cat="JJ">critical</tok></cons> <tok cat="NN">mechanism</tok></cons> <cons cat="VP"><tok cat="VBG">underlying</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">diversification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Ig</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1009092303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">mutation</tok></cons> <cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">germinal</tok> <tok cat="NN">center</tok> <tok cat="NN">reaction</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRP">it</tok></cons> <cons cat="SBAR" ref="i11" null="EXP"></cons></cons><cons cat="VP"><tok cat="VBZ">remains</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">matter</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">debate</tok></cons></cons></cons> <cons cat="SBAR" id="i11"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">mutation</tok> <tok cat="NN">machinery</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">targets</tok> <cons cat="NP"><tok cat="JJ">non-Ig</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1009092304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">studied</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">mutations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">5'</tok> <tok cat="JJ">noncoding</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Bcl6</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">subtypes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">lymphomas</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1009092305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="NP"><tok cat="JJ">frequent</tok> <tok cat="NN">hypermutation</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">follicular</tok> <tok cat="NN">lymphoma</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">25</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">59</tok></cons></cons></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">42</tok><tok cat="NN">%</tok></cons></cons></cons><tok cat="RRB">)</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">germinal</tok> <tok cat="NN">center</tok> <tok cat="NN">cell</tok> <tok cat="NN">origin</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">mucosa-associated</tok> <tok cat="JJ">lymphoid</tok> <tok cat="NN">tissue</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MALT</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">lymphoma</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">19</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">45</tok></cons></cons></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">42</tok><tok cat="NN">%</tok></cons></cons></cons><tok cat="RRB">)</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">postgerminal</tok> <tok cat="NN">center</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="PP"><cons cat="ADVP" adv="TMP"><tok cat="RB">only</tok> <tok cat="RB">occasionally</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">mantle</tok> <tok cat="NN">cell</tok> <tok cat="NN">lymphoma</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">1</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">21</tok></cons></cons></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">4.8</tok><tok cat="NN">%</tok></cons></cons></cons><tok cat="RRB">)</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">pregerminal</tok> <tok cat="NN">center</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@62@@oe@16-12-2010
1009092306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJS">Most</tok> <tok cat="NNS">mutations</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="PP" role="PRD"><tok cat="IN">outside</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">motifs</tok></cons> <cons cat="ADJP"><cons cat="ADVP"><tok cat="RB">potentially</tok></cons> <tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><cons cat="COMP" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">lymphomagenesis</tok></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="MD">may</tok><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">like</tok> <cons cat="NP"><tok cat="NN">Ig</tok> <tok cat="NN">mutation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VB">represent</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">inherent</tok> <tok cat="NN">feature</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">lymphoma</tok> <tok cat="NN">precursor</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1009092307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">normal</tok> <tok cat="NN">cell</tok> <tok cat="NNS">counterparts</tok></cons> <cons cat="VP"><tok cat="VBN">microdissected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">reactive</tok> <tok cat="NN">tonsil</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1009092308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="NN">Bcl6</tok> <tok cat="NN">mutation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">13</tok> <tok cat="IN">of</tok> <tok cat="CD">24</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">54</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons> <tok cat="NNS">clones</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">germinal</tok> <tok cat="NN">centre</tok></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">1</tok> <tok cat="IN">of</tok> <tok cat="CD">24</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">4</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons> <tok cat="NNS">clones</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">naive</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mantle</tok> <tok cat="NN">zone</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
1009092309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">frequency</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">distribution</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">nature</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">mutations</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">similar</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Ig</tok> <tok cat="NN">hypermutation</tok> <tok cat="NN">process</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1009092310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">show</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">unequivocal</tok> <tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">non-Ig</tok> <tok cat="NN">gene</tok> <tok cat="NN">hypermutation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">germinal</tok> <tok cat="NN">center</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">fresh</tok> <tok cat="NNS">insights</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">process</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">hypermutation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">lymphomagenesis</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1009093101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">1,25-Dihydroxyvitamin</tok> <tok cat="NN">D3</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">retinoic</tok> <tok cat="JJ">acid-resistant</tok> <tok cat="JJ">acute</tok> <tok cat="JJ">promyelocytic</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">UF-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p21(WAF1/CIP1)</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p27(KIP1)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1009093102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Retinoic</tok> <tok cat="NN">acid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RA</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">resistance</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">serious</tok> <tok cat="NN">problem</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">promyelocytic</tok> <tok cat="NN">leukemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">APL</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WP">who</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBG">receiving</tok> <cons cat="NP"><tok cat="JJ">all-trans</tok> <tok cat="NN">RA</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1009093103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">mechanisms</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">strategies</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i2" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">overcome</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">RA</tok> <tok cat="NN">resistance</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">APL</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="ADVP" ref="i2" null="T"></cons></cons></cons></cons></cons></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">still</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">unclear</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1009093104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">biologic</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">RA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">distinct</tok> <tok cat="NNS">families</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NNS">factors</tok></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">RA</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">RARs</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">retinoid</tok> <tok cat="NN">X</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">RXRs</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1009093105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="NNS">RXRs</tok></cons> <cons cat="VP"><tok cat="VBP">heterodimerize</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">1, 25-dihydroxyvitamin</tok> <tok cat="NN">D3</tok> <tok cat="LRB">[</tok><tok cat="NN">1,25(OH)2D3</tok><tok cat="RRB">]</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">VDR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">enabling</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">efficient</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1009093106@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">cyclin-dependent</tok> <tok cat="NN">kinase</tok> <tok cat="LRB">(</tok><tok cat="NN">cdk</tok><tok cat="RRB">)</tok> <tok cat="NN">inhibitor</tok> <tok cat="NN">p21(WAF1/CIP1)</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">vitamin</tok> <tok cat="JJ">D3-responsive</tok> <tok cat="NN">element</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">VDRE</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">1,25(OH)2D3</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">enhances</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p21(WAF1/CIP1)</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">selected</tok> <tok cat="JJ">myeloid</tok> <tok cat="JJ">leukemic</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
1009093107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP"><tok cat="VBN">established</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="NN">APL</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">UF-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">features</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">RA</tok> <tok cat="NN">resistance</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1009093108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">1,25(OH)2D3</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">growth</tok> <tok cat="NN">inhibition</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">G1</tok> <tok cat="NN">arrest</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">UF-1</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">toward</tok> <cons cat="NP"><tok cat="NNS">granulocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1009093109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">This</tok> <tok cat="JJ">1, 25(OH)2D3-induced</tok> <tok cat="NN">G1</tok> <tok cat="NN">arrest</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">enhanced</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">all-trans</tok> <tok cat="NN">RA</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
1009093110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Also</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">1, 25(OH)2D3</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="QP"><tok cat="CD">10(-10)</tok> <tok cat="TO">to</tok> <tok cat="CD">10(-7)</tok></cons> <tok cat="NN">mol/L</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">RA</tok></cons></cons></cons></cons></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">markedly</tok></cons> <tok cat="VBZ">inhibits</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NN">proliferation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">dose-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">time-dependent</tok></cons></cons> <tok cat="NN">manner</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1009093111@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i6" id="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">Associated</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">findings</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p21(WAF1/CIP1)</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p27(KIP1)</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1009093112@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Northern</tok> <tok cat="NN">blot</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">p21(WAF1/CIP1)</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p27(KIP1)</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok></cons></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1009093113@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Northern</tok> <tok cat="NN">blot</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="NN">p21(WAF1/CIP1)</tok> <tok cat="CC">and</tok> <tok cat="NN">p27(KIP1)</tok> <tok cat="NNS">transcripts</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">6</tok> <tok cat="NNS">hours</tok><tok cat="POS">'</tok></cons> <tok cat="NN">exposure</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">1,25(OH)2D3</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">then</tok></cons> <tok cat="VBD">decreased</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">basal</tok> <tok cat="NNS">levels</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">over</tok> <cons cat="NP"><tok cat="CD">48</tok> <tok cat="NNS">hours</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1009093114@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Western</tok> <tok cat="NN">blot</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">p21(WAF1/CIP1)</tok> <tok cat="NN">protein</tok> <tok cat="NNS">levels</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">increased</tok></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">became</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">detectable</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">12</tok> <tok cat="NNS">hours</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">1,25(OH)2D3</tok> <tok cat="NN">treatment</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p27(KIP1)</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="UCP" syn="COOD" role="PRD"><cons cat="ADJP" role="PRD"><cons cat="ADVP"><tok cat="RB">much</tok> <tok cat="RBR">more</tok></cons> <tok cat="JJ">gradual</tok></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="JJ">sustained</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">UF-1</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1009093115@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Interestingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">1, 25(OH)2D3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">RA</tok></cons></cons></cons></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">markedly</tok></cons> <tok cat="VBD">enhanced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p27(KIP1)</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">transcript</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok></cons></cons></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" ref="i10" id="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">1, 25(OH)2D3</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1009093116@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="JJ">exogenous</tok> <tok cat="NN">p27(KIP1)</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">enhance</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD11b</tok> <tok cat="NN">antigen</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="JJ">leukemic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1009093117@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">RA</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">G1</tok> <tok cat="NN">arrest</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">UF-1</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <tok cat="RB">however</tok><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">result</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p21(WAF1/CIP1)</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p27(KIP1)</tok> <tok cat="NN">transcript</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">RA-resistant</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
1009093118@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" id="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">Taken</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">together</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">1,25(OH)2D3</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cdk</tok> <tok cat="NNS">inhibitors</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i13"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i13" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">mediates</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">G1</tok> <tok cat="NN">arrest</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i14" role="SBJ"><tok cat="DT">this</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i14" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">RA-resistant</tok> <tok cat="NN">UF-1</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">toward</tok> <cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="NNS">granulocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1009094201@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP"><tok cat="JJ">Human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="JJ">virus-associated</tok></cons> <cons cat="NP"><tok cat="NN">Hodgkin</tok><tok cat="POS">'s</tok></cons> <tok cat="NN">disease</tok></cons> <cons cat="VP"><tok cat="VBZ">derives</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">post-germinal</tok> <tok cat="JJ">center</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
1009094202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">Human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="JJ">virus-associated</tok></cons> <cons cat="NP"><tok cat="NN">Hodgkin</tok><tok cat="POS">'s</tok></cons> <tok cat="NN">disease</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HIV-HD</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">displays</tok> <cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NNS">peculiarities</tok></cons> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i3"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" id="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HD</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">general</tok> <tok cat="NN">population</tok></cons></cons></cons></cons><cons cat="ADVP" ref="i3" null="T"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1009094203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok></cons> <cons cat="VP"><tok cat="VBP">include</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">overrepresentation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">clinically</tok> <tok cat="JJ">aggressive</tok></cons> <tok cat="JJ">histologic</tok> <tok cat="NNS">types</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">frequent</tok> <tok cat="NN">infection</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Reed-Sternberg</tok> <tok cat="LRB">(</tok><tok cat="NN">RS</tok><tok cat="RRB">)</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EBV</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1009094204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Recently</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">reported</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">histogenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HD</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">general</tok> <tok cat="NN">population</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">assessed</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">monitoring</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok> <tok cat="NN">pattern</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">BCL-6</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">germinal</tok> <tok cat="NN">center</tok> <tok cat="LRB">(</tok><tok cat="NN">GC</tok><tok cat="RRB">)</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD138/syndecan-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">syn-1</tok></cons><tok cat="RRB">)</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">proteoglycan</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">post-GC</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">terminal</tok></cons></cons> <tok cat="NN">B-cell</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@55@@oe@16-12-2010
1009094205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">applied</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="CD">two</tok> <tok cat="NNS">markers</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">study</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV-HD</tok> <tok cat="NN">histogenesis</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">correlated</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">expression</tok> <tok cat="NN">status</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">virologic</tok> <tok cat="NNS">features</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">disease</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1009094206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">RS</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="JJ">histologic</tok> <tok cat="NNS">categories</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV-HD</tok></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">consistently</tok></cons> <tok cat="VBP">display</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">BCL-6(-)/syn-1(+)</tok> <tok cat="NN">phenotype</tok></cons></cons> <tok cat="CC">and</tok> <tok cat="RB">thus</tok> <cons cat="VP"><tok cat="VBP">reflect</tok> <cons cat="NP"><tok cat="JJ">post-GC</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1009094207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">BCL-6(-)/syn-1(+)</tok><tok cat="NN">RS</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV-HD</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP"><tok cat="NN">CD40</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">surrounded</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD40</tok> <tok cat="JJ">ligand-positive</tok> <tok cat="LRB">(</tok><tok cat="JJ">CD40L+</tok><tok cat="RRB">)</tok> <tok cat="JJ">reactive</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">which</tok></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HD</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">general</tok> <tok cat="NN">population</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" ref="i6" id="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">thought</tok> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">disease</tok> <tok cat="NN">phenotype</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">CD40/CD40L</tok></cons> <tok cat="NNS">interactions</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
1009094208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Conversely</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">RS</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">virtually</tok> <tok cat="DT">all</tok></cons> <tok cat="NN">HIV-HD</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">EBV-encoded</tok> <tok cat="JJ">latent</tok> <tok cat="NN">membrane</tok> <tok cat="NN">protein</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LMP1</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">which</tok></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">being</tok> <cons cat="ADVP"><tok cat="RB">functionally</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">homologous</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">CD40</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" ref="i8" id="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">contribute</tok><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">part</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">modulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HIV-HD</tok> <tok cat="NN">phenotype</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
1009094701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Constitutive</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">adult</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
1009094702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">I</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HTLV-I</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">etiologic</tok> <tok cat="NN">agent</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ATL</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1009094703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">viral</tok> <tok cat="NN">protein</tok> <tok cat="NN">Tax</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">translocalization</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">NF-kappaB</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" id="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">proposed</tok> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">crucial</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transformation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">HTLV-I</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1009094704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HTLV-I</tok> <tok cat="NNS">genes</tok></cons> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><tok cat="NN">Tax</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">leukemic</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">ATL</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1009094705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">basis</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="RB">freshly</tok> <tok cat="VBN">isolated</tok> <tok cat="JJ">leukemic</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">ATL</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1009094706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">leukemic</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">ATL</tok> <tok cat="NNS">patients</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">like</tok> <cons cat="NP"><tok cat="JJ">HTLV-I-infected</tok> <tok cat="NN">T-cell</tok> <tok cat="NNS">lines</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBP">display</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">constitutive</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">degradation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IkappaBalpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1009094707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">Tax-expressing</tok> <tok cat="NN">T-cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">consisted</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">mostly</tok></cons> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p50/c-Rel</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="JJ">fresh</tok> <tok cat="NN">ATL</tok> <tok cat="NNS">samples</tok></cons> <cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP"><tok cat="NN">p50/p50</tok> <tok cat="CC">and</tok> <tok cat="NN">p50/p65</tok> <tok cat="NNS">heterodimers</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1009094708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="CD">One</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">line</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">ATL</tok> <tok cat="JJ">leukemic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">TL-Om1</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">displayed</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">constitutive</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">activity</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="VBN">enhanced</tok> <tok cat="NN">degradation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IkappaBalpha</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="PP"><tok cat="IN">despite</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">lack</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">detectable</tok> <tok cat="NN">Tax</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1009094709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Interestingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappaB</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">TL-Om1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">consists</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p50/p50</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p50/p65</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">like</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">fresh</tok> <tok cat="JJ">primary</tok> <tok cat="JJ">leukemic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1009094710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">Tax-independent</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">leukemic</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ATL</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">possibly</tok></cons> <tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">differential</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">subunit</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1009207601@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Apoptosis-resistant</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">deficiency</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">NF-kappaB-mediated</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Fas</tok> <tok cat="NN">ligand</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1009207602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NN">Apoptosis</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">TCR</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">CD4+</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">mostly</tok></cons> <tok cat="VBN">mediated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inducible</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Fas</tok> <tok cat="NN">ligand</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">FasL</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">primary</tok> <tok cat="NN">event</tok></cons> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">commitment</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">death</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1009207603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">gain</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJR">better</tok> <tok cat="NN">understanding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NNS">events</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">regulate</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i3" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">took</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">advantage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">our</tok> <cons cat="ADJP"><tok cat="RB">previously</tok> <tok cat="VBN">described</tok></cons> <tok cat="JJ">mutant</tok> <tok cat="NN">Jurkat</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1009207604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">deficient</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">FasL</tok> <tok cat="NN">expression</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">upon</tok> <cons cat="NP"><tok cat="NN">TCR</tok> <tok cat="NN">triggering</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">their</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">production</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">normal</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1009207605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="NN">FasL-</tok> <cons cat="NP" null="QSTN"></cons></cons><cons cat="NP" ref="i11" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">consensus</tok> <tok cat="NN">NF-kappaB- reporter</tok> <cons cat="NP" ref="i11" null="RNR"></cons></cons></cons><cons cat="NP" id="i11"><tok cat="NN">construct</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">inefficiently</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="VBN">compared</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">wild-type</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1009207606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inducible</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">FasL</tok> <tok cat="NN">reporter</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">abolished</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NNS">inhibitors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1009207607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">trace</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">deficit</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">mutant</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">inefficient</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">evidencing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">relevant</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">FasL</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
1009207608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">FasL</tok> <tok cat="CC">versus</tok> <tok cat="NN">cytokine</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">differentially</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">sensitive</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">deprivation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1009209101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NF-kappaB</tok></cons> <cons cat="VP"><tok cat="VBZ">regulates</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Fas/APO-1/CD95-</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">TCR- mediated</tok> <tok cat="NN">apoptosis</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
1009209102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">maintenance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">lymphocyte</tok> <tok cat="NN">homeostasis</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <tok cat="JJ">critical</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">normal</tok> <tok cat="JJ">immune</tok> <tok cat="NN">system</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1009209103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">NF-kappaB</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">protecting</tok> <cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">death</tok></cons> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">TNF</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons>@@@@1@19@@oe@16-12-2010
1009209104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NF-kappaB</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Fas/APO-1/CD95-mediated</tok> <tok cat="NN">death</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">major</tok> <tok cat="NN">pathway</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">death</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1009209105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Transfection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="NP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NNS">subunits</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p50</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">confers</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">resistance</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="JJ">Fas-mediated</tok> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1009209106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Reciprocally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">soluble</tok> <tok cat="NN">peptide</tok> <tok cat="NN">inhibitor</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">dominant</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">inhibitor</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IkappaB</tok></cons><tok cat="COMMA">,</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">makes</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="RBR">more</tok> <tok cat="JJ">susceptible</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">Fas-mediated</tok> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
1009209107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">soluble</tok> <tok cat="NN">peptide</tok> <tok cat="NN">inhibitor</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">rendered</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">a</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">hybridoma</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="RBR">more</tok> <tok cat="JJ">susceptible</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">TCR-mediated</tok> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1009209108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Correspondingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">transfection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p50</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">provided</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">considerable</tok> <tok cat="NN">protection</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">TCR-mediated</tok> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1009209109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">observations</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">corroborated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">studies</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Fas-mediated</tok> <tok cat="NN">death</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1009209110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Concanavalin</tok> <tok cat="JJ">A-activated</tok> <tok cat="NN">cycling</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">blasts</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">mice</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">transgenic</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">dominant</tok> <tok cat="NN">IkappaB</tok> <tok cat="NN">molecule</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">increased</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">sensitivity</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">Fas-mediated</tok> <tok cat="NN">apoptosis</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" id="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">down-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NNS">complexes</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
1009209111@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" role="SBJ"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">blocking</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">TNF</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP" tpc="TPC"><tok cat="PRP">itself</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">positive</tok> <tok cat="NN">regulator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="VBG">neutralizing</tok> <tok cat="NNS">antibodies</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">renders</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="RBR">more</tok> <tok cat="JJ">susceptible</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">anti-Fas-mediated</tok> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1009209112@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">summary</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><tok cat="JJ">compelling</tok> <tok cat="NN">evidence</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">NF-kappaB</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">protects</tok> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="JJ">Fas-mediated</tok> <tok cat="NN">death</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">likely</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">regulator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">homeostasis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">tolerance</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1009210901@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Interferon-beta</tok></cons> <cons cat="VP"><tok cat="VBZ">mediates</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">stromal</tok> <tok cat="NN">cell</tok> <tok cat="NN">rescue</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
1009210902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">resolution</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">immune</tok> <tok cat="NNS">responses</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">characterized</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">extensive</tok> <tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1009210903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">generate</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">maintain</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons></cons><cons cat="NP" id="i2"><tok cat="JJ">immunological</tok> <tok cat="NN">memory</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">some</tok> <tok cat="JJ">antigen-specific</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="MD">must</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">survive</tok></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">revert</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">resting</tok> <tok cat="NN">G0/G1</tok> <tok cat="NN">state</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1009210904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Cytokines</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">common</tok> <tok cat="NN">gamma</tok> <tok cat="NN">chain</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">promote</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">survival</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">blasts</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><tok cat="NN">proliferation</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1009210905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">soluble</tok> <tok cat="NNS">factors</tok></cons> <cons cat="VP"><tok cat="VBN">secreted</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">stromal</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><tok cat="NN">T</tok><tok cat="NN">cell</tok> <tok cat="NN">survival</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">resting</tok> <tok cat="NN">G0/G1</tok> <tok cat="NN">state</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1009210906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">interferon-beta</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">principal</tok> <tok cat="NN">mediator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">stromal</tok> <tok cat="JJ">cell-mediated</tok></cons> <tok cat="NN">T</tok><tok cat="NN">cell</tok> <tok cat="NN">rescue</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1009210907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Interferon-alpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><tok cat="NN">-beta</tok></cons></cons> <cons cat="VP"><tok cat="VBP">promote</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">reversion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">blast</tok> <tok cat="NN">T</tok><tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="VBG">resting</tok> <tok cat="NN">G0/G1</tok> <tok cat="NN">configuration</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="PDT">all</tok> <tok cat="DT">the</tok> <tok cat="JJ">characteristic</tok> <tok cat="NNS">features</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">stromal</tok> <tok cat="NN">cell</tok> <tok cat="NN">rescue</tok></cons></cons><tok cat="COLON">;</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NN">Bcl-XL</tok> <tok cat="NN">expression</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">low</tok> <tok cat="NN">Bcl-2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
1009210908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Type</tok> <tok cat="CD">I</tok> <tok cat="NNS">interferons</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">stromal</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBP">stimulate</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">apparently</tok> <tok cat="JJ">identical</tok></cons> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">STAT-1</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1009210909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">fundamental</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">persistence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">chronic</tok> <tok cat="NN">inflammation</tok></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">chronic</tok> <tok cat="NN">inflammation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">aberrant</tok> <tok cat="NN">consequence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">immunological</tok> <tok cat="NN">memory</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1009277501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">distinct</tok> <tok cat="JJ">intracellular</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">CD34+</tok> <tok cat="NN">progenitor</tok></cons> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">dendritic</tok> <tok cat="NN">cell</tok></cons></cons> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">human</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">model</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1009277502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Intracellular</tok> <tok cat="NNS">signals</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">mediate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">pluripotent</tok> <tok cat="JJ">hemopoietic</tok> <tok cat="NNS">progenitors</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">dendritic</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">DC</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">largely</tok> <tok cat="JJ">undefined</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1009277503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PKC</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">activation</tok></cons> <tok cat="LRB">(</tok><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">ester</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PMA</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons></cons><tok cat="RRB">)</tok></cons> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">human</tok> <tok cat="JJ">CD34+</tok> <tok cat="JJ">hemopoietic</tok> <tok cat="NN">progenitor</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HPC</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="NN">DC</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
1009277504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="VP"><tok cat="VBP">find</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="JJ">cytokine-driven</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">granulocyte-macrophage</tok> <tok cat="NN">CSF</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons></cons><tok cat="RRB">)</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">CD34+</tok> <tok cat="NN">HPC</tok></cons><tok cat="TO">--&gt;</tok><cons cat="NP"><tok cat="NN">DC</tok></cons></cons> <tok cat="NN">differentiation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">preferentially</tok></cons> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">inhibitors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PKC</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1009277505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">further</tok></cons> <tok cat="VB">identify</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NNS">signals</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">downstream</tok> <tok cat="NNS">events</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">CD34+</tok> <tok cat="NN">HPC</tok></cons><tok cat="TO">--&gt;</tok><cons cat="NP"><tok cat="NN">DC</tok></cons></cons> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="NP" id="i3" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">characterized</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">human</tok> <tok cat="JJ">leukemic</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">model</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">process</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1009277506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">CD34+</tok> <tok cat="JJ">myelomonocytic</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">KG1</tok></cons> <cons cat="VP"><tok cat="VBZ">differentiates</tok> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="JJ">dendritic-like</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">granulocyte-macrophage</tok> <tok cat="NN">CSF</tok></cons> <tok cat="CC">plus</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PMA</tok></cons> <tok cat="LRB">(</tok><cons cat="PP"><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" ref="i4" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP" ref="i4" null="RNR"></cons></cons></cons><cons cat="NP" syn="COOD" id="i4"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">calcium</tok> <tok cat="NN">ionophore</tok> <tok cat="NN">ionomycin</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">different</tok> <tok cat="NNS">stimuli</tok></cons> <cons cat="VP"><tok cat="VBG">mediating</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">aspects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">process</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
1009277507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Phenotypic</tok> <tok cat="NN">DC</tok> <tok cat="NNS">characteristics</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">KG1</tok> <tok cat="JJ">dendritic-like</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">include</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">morphology</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="RB">loosely</tok> <tok cat="JJ">adherent</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">long</tok> <tok cat="NN">neurite</tok> <tok cat="NNS">processes</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="JJ">I+/MHC</tok> <tok cat="JJ">IIbright/CD83+/CD86+/CD14-</tok> <tok cat="NN">surface</tok> <tok cat="NN">Ag</tok> <tok cat="NN">expression</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">RelB</tok> <tok cat="CC">and</tok> <tok cat="NN">DC-CK1</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1009277508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Functional</tok> <tok cat="NN">DC</tok> <tok cat="NNS">characteristics</tok></cons> <cons cat="VP"><tok cat="VBP">include</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">fluid</tok> <tok cat="NN">phase</tok> <tok cat="NN">macromolecule</tok> <tok cat="NN">uptake</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">FITC-dextran</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBG">resting</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1009277509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">Comparison</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">KG1</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">PMA-unresponsive</tok> <tok cat="NN">subline</tok> <tok cat="NN">KG1a</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">reveals</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">differences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">TNF</tok> <tok cat="NNS">receptors</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">2</tok></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><tok cat="NN">PKC</tok> <tok cat="NNS">isoforms</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">beta</tok> <tok cat="CD">I</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">beta</tok> <tok cat="CD">II</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">mu</tok></cons></cons></cons><tok cat="COLON">;</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">RelB</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">components/pathways</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">DC</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
1009277510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Together</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="CC">or</tok> <tok cat="NN">phorbol</tok> <tok cat="NN">ester</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">KG1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">model</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">CD34+</tok> <tok cat="NN">HPC</tok></cons> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">DC</tok></cons></cons> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">specific</tok> <tok cat="JJ">intracellular</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="VP"><tok cat="VBP">mediate</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NNS">events</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">DC</tok> <tok cat="NN">lineage</tok> <tok cat="NN">commitment</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
1009278301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">IL-2-mediated</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok> <tok cat="NN">progression</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">require</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="CC">or</tok> <tok cat="VBG">activating</tok> <tok cat="NN">protein-1</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1009278302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">growth</tok> <tok cat="NN">hormone</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">major</tok> <tok cat="NN">growth</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="VBG">developing</tok> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1009278303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="NN">IL-2</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="UCP" syn="COOD"><cons cat="CONJP"><tok cat="RB">not</tok> <tok cat="RB">only</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">cycle</tok> <tok cat="NN">progression</tok></cons></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">also</tok></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">protect</tok> <cons cat="NP"><tok cat="JJ">Ag-activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="VBN">programmed</tok> <tok cat="NN">cell</tok> <tok cat="NN">death</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1009278304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="CC">and/or</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">activating</tok> <tok cat="NN">protein-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AP-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">extremely</tok> <tok cat="JJ">important</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">blocking</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1009278305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">either</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">both</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">survival</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">cycle</tok> <tok cat="NN">progression</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="ADJP"><tok cat="JJ">responsive</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">growth</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="CC">and</tok> <tok cat="NN">AP-1</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
1009278306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">current</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">clearly</tok></cons> <tok cat="VBZ">demonstrates</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-2</tok></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok> <tok cat="NN">degradation</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">activation</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" id="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">respond</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBG">entering</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBG">avoiding</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
1009278307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Similarly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">IL-2</tok></cons> <cons cat="VP" syn="COOD"><tok cat="CC">neither</tok> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="NN">JNK</tok></cons></cons> <tok cat="CC">nor</tok> <cons cat="VP"><tok cat="VBZ">increases</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">consensus</tok> <tok cat="NN">o-tetradecanoylphorbol</tok> <tok cat="NN">13-acetate</tok> <tok cat="LRB">(</tok><tok cat="NN">TPA</tok><tok cat="RRB">)</tok> <tok cat="NN">response</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1009278308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">On</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">other</tok> <tok cat="NN">hand</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">growth</tok> <tok cat="NN">factor</tok></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT5</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <tok cat="VBN">demonstrated</tok><cons cat="NP" ref="i8" null="NONE"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1009278309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="CC">neither</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="CC">nor</tok> <tok cat="NN">AP-1</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">IL-2-mediated</tok> <tok cat="NN">survival</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">cycle</tok> <tok cat="NN">progression</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="JJ">primary</tok> <tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1009280101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Impaired</tok> <tok cat="JJ">fetal</tok> <tok cat="NN">thymocyte</tok> <tok cat="NN">development</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">efficient</tok> <tok cat="JJ">adenovirus-mediated</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
1009280102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">introduce</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">new</tok> <tok cat="JJ">experimental</tok> <tok cat="NN">system</tok></cons> <cons cat="VP"><tok cat="VBG">combining</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">adenovirus-mediated</tok> <tok cat="NN">gene</tok> <tok cat="NN">transfer</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">fetal</tok> <tok cat="JJ">thymic</tok> <tok cat="NN">organ</tok> <tok cat="NN">culture</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">FTOC</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1009280103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">system</tok></cons> <cons cat="VP"><tok cat="VBD">allowed</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">us</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="TO">to</tok> <cons cat="ADVP"><tok cat="RB">efficiently</tok></cons> <cons cat="VP"><tok cat="VB">express</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBG">developing</tok> <tok cat="NNS">thymocytes</tok></cons></cons> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">mutant</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">inhibitor</tok> <tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">alpha</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">mut-I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">study</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">maturation</tok> <tok cat="NNS">defects</tok></cons> <cons cat="VP"><tok cat="VBG">occurring</tok> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i1"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">fetal</tok> <tok cat="NN">development</tok></cons></cons><cons cat="ADVP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
1009280104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="JJ">Fetal</tok> <tok cat="NNS">thymocytes</tok></cons> <cons cat="VP"><tok cat="VBN">infected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">adenovirus</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="NN">mut-I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">develop</tok> <cons cat="ADVP"><tok cat="RB">normally</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">until</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">CD44-CD25+</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">CD4-CD8-</tok> <tok cat="JJ">double-negative</tok></cons></cons> <tok cat="NN">stage</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RBR">more</tok> <tok cat="JJ">mature</tok></cons> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">double-positive</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">single-positive</tok></cons></cons> <tok cat="NNS">populations</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <tok cat="VBN">decreased</tok><cons cat="NP" ref="i4" null="NONE"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1009280105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Proliferation</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">measured</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">percentage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">cycle</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">appeared</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">normal</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="SINV"><tok cat="VBD">did</tok> <cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">rearrangement</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">TCR</tok> <tok cat="NN">beta-chain</tok></cons></cons></cons><cons cat="VP" null="QSTN"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1009280106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">apoptosis</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="RB">much</tok> <tok cat="JJR">higher</tok></cons> <cons cat="PP" ref="i7" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">FTOC</tok></cons> <cons cat="VP"><tok cat="VBN">infected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">adenovirus</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="NN">mut-I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="PP" id="i7"><tok cat="IN">than</tok> <cons cat="FRAG"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">FTOC</tok></cons> <cons cat="VP"><tok cat="VBN">infected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">control</tok> <tok cat="NN">virus</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1009280107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i8" id="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">Taken</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">together</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">crucial</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">ensuring</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">survival</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">thymocytes</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">early</tok> <tok cat="NNS">stages</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">development</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1009280501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Differential</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">4E-BP1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">4E-BP2</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">repressors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">translation</tok> <tok cat="NN">initiation</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">cell</tok> <tok cat="NN">differentiation</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1009280502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="JJ">Human</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">accompanied</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">proliferation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1009280503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Because</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">translation</tok> <tok cat="NN">rate</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">determinant</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">proliferation</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i2" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">translation</tok> <tok cat="NN">initiation</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">cell</tok> <tok cat="NN">differentiation</tok></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HL-60</tok> <tok cat="JJ">promyelocytic</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">U-937</tok> <tok cat="JJ">monoblastic</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
1009280504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">translation</tok> <tok cat="NN">rate</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i4"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">differentiate</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">along</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">monocytic/macrophage</tok> <tok cat="NN">pathway</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">along</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">granulocytic</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i4" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1009280505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">synthesis</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">correlates</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">repressors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">translation</tok> <tok cat="NN">initiation</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">4E-BP1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">4E-BP2</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1009280506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HL-60</tok> <tok cat="CC">and</tok> <tok cat="NN">U-937</tok> <tok cat="NN">cell</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="NNS">monocytes/macrophages</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">PMA</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">dephosphorylation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">consequent</tok> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">4E-BP1</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1009280507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">Dephosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">4E-BP1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">also</tok></cons> <tok cat="VBN">observed</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i7"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="NN">U-937</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">differentiate</tok> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="NNS">monocytes/macrophages</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="VBG">following</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">DMSO</tok></cons></cons></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i7" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1009280508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HL-60</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">DMSO</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">granulocytic</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">decreases</tok> <cons cat="NP"><tok cat="NN">4E-BP1</tok> <tok cat="NN">amount</tok></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">affecting</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">phosphorylation</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <tok cat="VBZ">increases</tok> <cons cat="NP"><tok cat="NN">4E-BP2</tok> <tok cat="NN">amount</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
1009280509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i11" id="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">Taken</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">together</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i11" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">differential</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">translational</tok> <tok cat="NN">machinery</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">specific</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">monocytic/macrophage</tok> <tok cat="NN">pathway</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">granulocytic</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1009282501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">Glucocorticoids</tok></cons> <cons cat="VP"><tok cat="VBP">promote</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nonphlogistic</tok> <tok cat="NN">phagocytosis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">apoptotic</tok> <tok cat="NNS">leukocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@16-12-2010
1009282502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Phagocyte</tok> <tok cat="NN">recognition</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">uptake</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">nonphlogistic</tok> <tok cat="NN">degradation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">neutrophils</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">leukocytes</tok></cons> <cons cat="VP"><tok cat="VBG">undergoing</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">promote</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">resolution</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">inflammation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1009282503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBD">assessed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">anti-inflammatory</tok> <tok cat="NNS">glucocorticoids</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">leukocyte</tok> <tok cat="NN">clearance</tok> <tok cat="NN">mechanism</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1009282504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Pretreatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="LQT">"</tok><tok cat="JJ">semimature</tok><tok cat="RQT">"</tok> <tok cat="JJ">5-day</tok> <tok cat="JJ">human</tok> <tok cat="JJ">monocyte-derived</tok> <tok cat="NNS">macrophages</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">M</tok> <tok cat="NN">phi</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="CD">24</tok> <tok cat="NN">h</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">methylprednisolone</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">dexamethasone</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">hydrocortisone</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nonglucocorticoid</tok> <tok cat="NNS">steroids</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">aldosterone</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">estradiol</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">progesterone</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">potentiated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phagocytosis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">apoptotic</tok> <tok cat="NNS">neutrophils</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
1009282505@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">effects</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">specific</tok></cons> <cons cat="SBAR"><tok cat="IN">in</tok> <tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">potentiated</tok> <tok cat="NN">phagocytosis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">apoptotic</tok> <tok cat="NNS">neutrophils</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">completely</tok></cons> <tok cat="VBN">blocked</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok> <tok cat="NN">antagonist</tok> <tok cat="NN">RU38486</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">glucocorticoids</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">promote</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">5-day</tok> <tok cat="NN">M</tok> <tok cat="NN">phi</tok> <tok cat="NN">ingestion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">opsonized</tok> <tok cat="NNS">erythrocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1009282506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="JJ">Similar</tok> <tok cat="JJ">glucocorticoid-mediated</tok> <tok cat="NN">potentiation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">5-day</tok> <tok cat="NN">M</tok> <tok cat="NN">phi</tok> <tok cat="NN">uptake</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">alternative</tok> <tok cat="JJ">apoptotic</tok> <tok cat="LQT">"</tok><tok cat="NNS">targets</tok><tok cat="RQT">"</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">eosinophils</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">uptake</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">apoptotic</tok> <tok cat="NNS">neutrophils</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">alternative</tok> <tok cat="NNS">phagocytes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">glomerular</tok> <tok cat="JJ">mesangial</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">M</tok> <tok cat="NN">phi</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">elicited</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">peritoneum</tok></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">bone</tok> <tok cat="NN">marrow</tok></cons></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@52@@oe@16-12-2010
1009282507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Importantly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">methylprednisolone-mediated</tok> <tok cat="NN">enhancement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">uptake</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">apoptotic</tok> <tok cat="NNS">neutrophils</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">trigger</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">release</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">chemokines</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-8</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">monocyte</tok> <tok cat="NN">chemoattractant</tok> <tok cat="NN">protein-1</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1009282508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="JJ">longer-term</tok> <tok cat="NN">potentiation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">methylprednisolone</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBG">maturing</tok> <tok cat="JJ">human</tok> <tok cat="JJ">monocyte-derived</tok> <tok cat="NN">M</tok> <tok cat="NN">phi</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="JJR">greater</tok> <tok cat="NNS">increases</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">5-day</tok> <tok cat="NN">M</tok> <tok cat="NN">phi</tok> <tok cat="NN">uptake</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">apoptotic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBG">being</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i4" null="NONE"></cons></cons></cons></cons></cons><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJR">earlier</tok> <tok cat="NNS">glucocorticoids</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">added</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">monocyte</tok> <tok cat="NN">maturation</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="NN">M</tok> <tok cat="NN">phi</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
1009282509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">potentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nonphlogistic</tok> <tok cat="NN">clearance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">apoptotic</tok> <tok cat="NNS">leukocytes</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NNS">phagocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">hitherto</tok> <tok cat="JJ">unrecognized</tok></cons> <tok cat="NN">property</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">potential</tok> <tok cat="NNS">implications</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">therapies</tok></cons> <cons cat="VP"><tok cat="VBN">aimed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">promoting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">resolution</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NNS">diseases</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
1009656101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD40</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">immunogenic</tok> <tok cat="JJ">human</tok> <tok cat="JJ">malignant</tok> <tok cat="NNS">melanomas</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">augments</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <cons cat="ADJP"><tok cat="JJ">cytotoxic</tok> <tok cat="NN">T</tok> <tok cat="JJ">lymphocyte-mediated</tok></cons> <tok cat="NN">lysis</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1009656102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">functional</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD40</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">malignant</tok> <tok cat="NNS">melanomas</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">MMs</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1009656103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Comparison</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">specimen</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">MM</tok> <tok cat="NN">precursor</tok> <tok cat="NNS">lesions</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NNS">tumors</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">metastases</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="NN">CD40</tok> <tok cat="NN">surface</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">down-regulated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">progression</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1009656104@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="NN">CD40</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">confirmed</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">7</tok> <tok cat="JJ">human</tok> <tok cat="NN">MM</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="VP"><tok cat="VBN">established</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">immunogenic</tok> <tok cat="JJ">primary</tok> <tok cat="NNS">tumors</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NNS">metastases</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">11</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="VP"><tok cat="VBN">established</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">advanced</tok> <tok cat="NNS">stages</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="NN">CD40</tok> <tok cat="JJ">negative</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1009656105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="NN">CD40</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">enhanced</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">CD40-positive</tok> <tok cat="NN">MM</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor-alpha</tok></cons></cons></cons></cons></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="LRB">(</tok><tok cat="NN">IL</tok><tok cat="RRB">)</tok><tok cat="NN">-1beta</tok> <tok cat="CC">or</tok> <tok cat="NN">CD40</tok> <tok cat="NN">triggering</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1009656106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">CD40</tok> <tok cat="NN">ligation</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">MM</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">CD40L-transfected</tok> <tok cat="JJ">murine</tok> <tok cat="NNS">L-cells</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">soluble</tok> <tok cat="NN">CD40L</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">up-regulated</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">intercellular</tok> <tok cat="NN">adhesion</tok> <tok cat="NN">molecule-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">I</tok> <tok cat="CC">and</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">secretion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-6</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-8</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor-a</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">granulocyte</tok> <tok cat="NN">macrophage</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <tok cat="RB">also</tok> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">rapid</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappaB</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@61@@oe@16-12-2010
1009656107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">CD40</tok> <tok cat="NN">ligation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">HLA-A2+</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">MelanA/MART1+</tok></cons></cons> <tok cat="NN">MM</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">enhanced</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">susceptibility</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">lysis</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">HLA-A2-restricted</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">MelanA/MART-1-specific</tok></cons></cons> <tok cat="NN">CTL</tok> <tok cat="NN">clone</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1009656108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">CD40</tok> <tok cat="NN">ligation</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">growth</tok> <tok cat="NN">inhibition</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">MM</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1009656109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">CD40-CD40L</tok> <tok cat="NNS">interactions</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBG">augmenting</tok> <cons cat="NP"><tok cat="NN">antitumor</tok> <tok cat="NN">immunity</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBG">inducing</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">some</tok> <tok cat="JJ">CD40-positive</tok> <tok cat="JJ">immunogenic</tok> <tok cat="JJ">human</tok> <tok cat="NNS">MMs</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1009657401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Glucocorticoid-induced</tok> <tok cat="NN">cell</tok> <tok cat="NN">death</tok></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">autoinduction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">leukemic</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1009657402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <tok cat="NN">contrast</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">negative</tok> <tok cat="NN">autoregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GR</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">expression</tok></cons></cons> <cons cat="VP"><tok cat="VBN">seen</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJS">most</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">tissues</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i1" role="SBJ"><tok cat="NN">GR</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">positively</tok></cons> <tok cat="VBN">autoregulated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">leukemic</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">cells</tok></cons> <cons cat="ADJP"><tok cat="JJ">sensitive</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">glucocorticoid-induced</tok> <tok cat="NN">cell</tok> <tok cat="NN">death</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
1009657403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">positive</tok> <tok cat="NN">autoregulation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">necessary</tok> <tok cat="NN">component</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">glucocorticoid-induced</tok> <tok cat="NN">cell</tok> <tok cat="NN">death</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">wild-type</tok> <tok cat="NN">GR</tok> <tok cat="NN">gene</tok></cons> <cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">tetracycline-regulated</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">stably</tok></cons> <cons cat="VP"><tok cat="VBN">transfected</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">glucocorticoid-resistant</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">lacking</tok> <cons cat="NP"><tok cat="JJ">endogenous</tok> <tok cat="JJ">functional</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
1009657404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Transfectants</tok></cons> <cons cat="VP"><tok cat="VBN">grown</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">presence</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">tetracycline</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="RB">about</tok> <tok cat="CD">15,000</tok></cons> <tok cat="NNS">receptors/cell</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">value</tok></cons> <cons cat="ADJP"><tok cat="RB">approximately</tok> <tok cat="JJ">equal</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">basal</tok> <tok cat="NN">level</tok> <tok cat="NN">GR</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">glucocorticoid-sensitive</tok> <tok cat="NN">6TG1.1</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">before</tok> <cons cat="NP"><tok cat="NN">steroid</tok> <tok cat="NN">treatment</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1009657405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Under</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">conditions</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">dexamethasone</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">minimal</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">growth</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">elicited</tok> <cons cat="NP"><tok cat="JJ">little</tok> <tok cat="JJ">internucleosomal</tok> <tok cat="NN">DNA</tok> <tok cat="NN">fragmentation</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok> <tok cat="NN">perturbation</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1009657406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <tok cat="DT">the</tok> <tok cat="NN">absence</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">tetracycline</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">GR</tok> <tok cat="NN">mRNA</tok> <tok cat="CC">and</tok> <tok cat="NN">protein</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="ADVP"><tok cat="RB">2-3-fold</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">48,000</tok> <tok cat="NNS">receptors</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">level</tok></cons> <cons cat="ADJP"><tok cat="RB">nearly</tok> <tok cat="JJ">equivalent</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="ADJP"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">6TG1.1</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">18</tok> <tok cat="NN">h</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">autoinduction</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
1009657407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Under</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">conditions</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">dexamethasone</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><cons cat="ADVP"><tok cat="RB">markedly</tok></cons> <tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">growth</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><tok cat="NN">G1</tok> <tok cat="NN">arrest</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><tok cat="JJ">significant</tok> <tok cat="JJ">internucleosomal</tok> <tok cat="NN">DNA</tok> <tok cat="NN">fragmentation</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1009657408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">studies</tok></cons> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="JJ">basal</tok> <tok cat="NN">level</tok> <tok cat="NN">GR</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">inadequate</tok> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">mediate</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">glucocorticoid-induced</tok> <tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">glucocorticoid-sensitive</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">positive</tok> <tok cat="NN">autoregulation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">necessary</tok> <tok cat="NN">component</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">process</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
1009778801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">Jeg-3</tok> <tok cat="JJ">human</tok> <tok cat="JJ">choriocarcinoma-induced</tok></cons> <tok cat="NN">immunosuppression</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">downregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">interleukin-2</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">interleukin-2</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha-chain</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">Jak/Stat</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
1009778802@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">PROBLEM</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">immunosuppressive</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">immunosuppression-inducing</tok></cons></cons> <tok cat="NNS">capacities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Jeg-3</tok> <tok cat="JJ">human</tok> <tok cat="NN">choriocarcinoma</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NNS">supernatants</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">HCSs</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <tok cat="RB">not</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">yet</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">completely</tok></cons> <tok cat="VBN">understood</tok><cons cat="NP" ref="i1" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@26@@oe@16-12-2010
1009778803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">influence</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="LRB">(</tok><tok cat="NN">IL</tok><tok cat="RRB">)</tok><tok cat="CD">-2</tok><tok cat="COMMA">,</tok> <tok cat="NN">IL-4</tok> <tok cat="CC">and</tok> <tok cat="NN">interferon</tok> <tok cat="LRB">(</tok><tok cat="NN">IFN</tok><tok cat="RRB">)</tok><tok cat="NN">-gamma</tok> <tok cat="NN">production</tok></cons><tok cat="COLON">;</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">receptor</tok> <tok cat="LRB">(</tok><tok cat="NN">IL-2R</tok><tok cat="RRB">)</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">alpha-</tok><cons cat="NP" null="QSTN"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">beta-</tok><cons cat="NP" null="QSTN"></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">gamma-chain</tok></cons></cons></cons><tok cat="COLON">;</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok> <tok cat="NNS">molecules</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Janus</tok> <tok cat="NN">kinase</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="LRB">(</tok><tok cat="NN">Jak</tok><tok cat="RRB">)</tok><tok cat="CD">1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Jak3</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NNS">transducers</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">activators</tok> <tok cat="IN">of</tok> <tok cat="NN">transcription</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="LRB">(</tok><tok cat="NN">Stat</tok><tok cat="RRB">)</tok><tok cat="CD">1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Stat3</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Stat5</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">should</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">investigated</tok><cons cat="NP" ref="i2" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@63@@oe@16-12-2010
1009778804@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">METHOD</tok></cons> <cons cat="PP"><tok cat="IN">OF</tok> <cons cat="NP"><tok cat="NN">STUDY</tok></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">For</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">assessment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL</tok> <tok cat="NN">production</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="JJ">whole</tok> <tok cat="JJ">peripheral</tok> <tok cat="JJ">venous</tok> <tok cat="NN">blood</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NNS">donors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">phorbol-myristate-acetate</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">ionomycine</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1009778805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="NN">Secretion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">ILs</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">monensine</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@16-12-2010
1009778806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="JJ">Intracellular</tok> <tok cat="NNS">ILs</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">flow</tok> <tok cat="NN">cytometry</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@16-12-2010
1009778807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">For</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="CC">and</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok> <tok cat="NN">molecule</tok> <tok cat="NN">analysis</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phytohemagglutinin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PHA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1009778808@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="NN">IL-2R</tok> <tok cat="NNS">chains</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">measured</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP" id="i8"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">flow</tok> <tok cat="NN">cytometry</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons map="i7" cat="NP" role="SBJ"><tok cat="NN">Jaks/Stats</tok></cons> <cons map="i8" cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">sodium</tok> <tok cat="NN">dodecyl</tok> <tok cat="NN">sulfate</tok> <tok cat="NN">polyacrylamide</tok> <tok cat="NN">gel</tok> <tok cat="NN">electrophoresis</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SDS-PAGE</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Western</tok> <tok cat="NN">blot</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1009778809@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">RESULTS</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Phorbol-myristate-acetate</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">ionomycine</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBP">increase</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">percent-age</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">IL-2+</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">an</tok> <tok cat="JJ">additional</tok> <cons cat="ADJP"><tok cat="CD">50</tok><tok cat="NN">%</tok></cons> <tok cat="NNS">HCSs</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <tok cat="VBZ">suppresses</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">percentage</tok></cons> <cons cat="PP"><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="TO">to</tok><cons cat="NP" ref="i9" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">below</tok> <cons cat="NP" ref="i9" null="RNR"></cons></cons></cons><cons cat="NP" id="i9"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">unstimulated</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1009778810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="NN">IFN-gamma</tok> <tok cat="NN">production</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <tok cat="VBN">decreased</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NNS">HCSs</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">some</tok> <tok cat="NNS">cases</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">others</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1009778811@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PHA</tok></cons> <cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha-</tok><tok cat="COMMA">,</tok> <tok cat="NN">beta-</tok><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <tok cat="NN">gamma-chain</tok> <tok cat="NN">expression</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok> <tok cat="NNS">molecules</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Jak1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Jak3</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Stat1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Stat3</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Stat5</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1009778812@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP"><tok cat="CD">50</tok><tok cat="NN">%</tok></cons> <tok cat="NN">HCS</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">downregulates</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">alpha-chain</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">slightly</tok></cons> <tok cat="VBZ">upregulates</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">beta-chain</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1009778813@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="NN">Jak1</tok><tok cat="COMMA">,</tok> <tok cat="NN">Jak3</tok><tok cat="COMMA">,</tok> <tok cat="NN">Stat1</tok><tok cat="COMMA">,</tok> <tok cat="NN">Stat3</tok><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <tok cat="NN">Stat5</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">suppressed</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><cons cat="ADVP"><tok cat="RB">approximately</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="TO">to</tok><cons cat="NP" ref="i12" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">below</tok> <cons cat="NP" ref="i12" null="RNR"></cons></cons></cons><cons cat="NP" id="i12"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">unstimulated</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1009778814@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">CONCLUSIONS</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HCS</tok></cons> <cons cat="ADVP"><tok cat="RB">forcefully</tok></cons> <cons cat="VP"><tok cat="VBZ">blocks</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2R</tok> <tok cat="NN">alpha-chain</tok></cons><tok cat="COLON">;</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Jak1</tok><tok cat="COMMA">,</tok> <tok cat="NN">Jak3</tok><tok cat="COMMA">,</tok> <tok cat="NN">Stat1</tok><tok cat="COMMA">,</tok> <tok cat="NN">Stat3</tok><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <tok cat="NN">Stat5</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1009778815@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i13" role="SBJ"><tok cat="DT">The</tok> <tok cat="VBN">observed</tok> <tok cat="NNS">phenomena</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">caused</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">downregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">IL-2R</tok> <tok cat="NN">regulation</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">key</tok> <tok cat="NN">role</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expansion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">choriocarcinoma</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">possibly</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">survival</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">fetal</tok> <tok cat="NN">allograft</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
1009860601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Characterization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">gene</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">pterin</tok> <tok cat="NN">carbinolamine</tok> <tok cat="NN">dehydratase/dimerization</tok> <tok cat="NN">cofactor</tok> <tok cat="IN">of</tok> <tok cat="NN">HNF1</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1009860602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Pterin</tok> <tok cat="NN">carbinolamine</tok> <tok cat="NN">dehydratase/dimerization</tok> <tok cat="NN">cofactor</tok> <tok cat="IN">of</tok> <tok cat="NN">HNF1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PCD/DCoH</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <tok cat="JJ">dual-function</tok> <tok cat="NN">protein</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1009860603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytoplasm</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">acts</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">dehydratase</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regeneration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tetrahydrobiopterin</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cofactor</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">aromatic</tok> <tok cat="NN">amino</tok> <tok cat="NN">acid</tok> <tok cat="NNS">hydroxylases</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1009860604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">functions</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">dimerization</tok> <tok cat="NN">cofactor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HNF1</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">increases</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HNF1</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1009860605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">deepen</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">our</tok> <tok cat="NN">understanding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">characterized</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">tissues</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1009860606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Human</tok> <tok cat="NN">PCD/DCoH</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><cons cat="ADVP"><tok cat="RB">predominantly</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">liver</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">kidney</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">significant</tok> <tok cat="NNS">amounts</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">testis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">ovary</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">trace</tok> <tok cat="NNS">amounts</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">lung</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">undetectable</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">whole</tok> <tok cat="NN">brain</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">heart</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">spleen</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1009860607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">all</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" id="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">examined</tok><cons cat="NP" ref="i3" null="NONE"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1009860608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Importantly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HeLa</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">lack</tok> <cons cat="NP"><tok cat="NN">HNF1</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytoplasm</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">fibroblasts</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">little</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="DT">no</tok></cons></cons> <tok cat="NN">tetrahydrobiopterin</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1009860609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">PCD/DCoH</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">liver</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">nonhepatic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok></cons></cons> <tok cat="NNS">levels</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1009860610@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">liver</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="RB">only</tok> <tok cat="RB">fourfold</tok> <tok cat="JJR">higher</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">keratinocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">fibroblasts</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">hepatic</tok> <tok cat="NN">PCD/DCoH</tok> <tok cat="NN">protein</tok> <tok cat="NN">level</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="RB">20-fold</tok> <tok cat="JJR">higher</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="JJ">human</tok> <tok cat="JJ">epidermal</tok> <tok cat="NNS">keratinocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">dermal</tok> <tok cat="NNS">fibroblasts</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
1009860611@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Cloning</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">5'</tok> <tok cat="CC">and</tok> <tok cat="JJ">3'</tok> <tok cat="JJ">untranslated</tok> <tok cat="NN">region</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">UTR</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">keratinocyte</tok> <tok cat="NN">PCD/DCoH</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">53</tok> <tok cat="NN">bp</tok> <tok cat="JJR">more</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">GC-rich</tok> <tok cat="JJ">5'</tok> <tok cat="JJ">untranslated</tok> <tok cat="NN">sequence</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">published</tok> <tok cat="NN">liver</tok> <tok cat="NN">PCD/DCoH</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1009860612@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="FW">In</tok> <tok cat="FW">vitro</tok> <tok cat="NN">transcription</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">translation</tok> <tok cat="NN">analysis</tok></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJR">longer</tok> <tok cat="JJ">5'</tok> <tok cat="NN">UTR</tok></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="RB">about</tok> <tok cat="DT">a</tok> <tok cat="CD">35</tok></cons><tok cat="NN">%</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">translation</tok> <tok cat="NN">efficiency</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1009860613@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">human</tok> <tok cat="NN">PCD/DCoH</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="CONJP"><tok cat="RB">not</tok> <tok cat="RB">only</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i7"><tok cat="WRB">where</tok></cons> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="NN">tetrahydrobiopterin</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">synthesized</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="ADVP" ref="i7" null="T"></cons></cons></cons></cons><tok cat="CC">or</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HNF1</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="ADVP" ref="i7" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <tok cat="RB">widely</tok> <tok cat="VBN">distributed</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1009860614@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="PRPP">Its</tok> <tok cat="JJ">differential</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">different</tok> <cons cat="NP"><tok cat="NNS">tissues</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="CONJP"><tok cat="RB">not</tok> <tok cat="RB">only</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">level</tok></cons></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">also</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">translational</tok> <tok cat="NN">level</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1010100101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Essential</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">alveolar</tok> <tok cat="NNS">macrophages</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">intrapulmonary</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
1010100102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Acute</tok> <tok cat="JJ">inflammatory</tok> <tok cat="NN">injury</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">rat</tok> <tok cat="NN">lung</tok></cons></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">deposition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">immunoglobulin</tok> <tok cat="NN">G</tok> <tok cat="JJ">immune</tok> <tok cat="NNS">complexes</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">cytokines</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">chemokines</tok></cons></cons></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="LRB">(</tok><tok cat="NN">NF</tok><tok cat="RRB">)</tok><tok cat="NN">-kappaB</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1010100103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">There</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">little</tok> <tok cat="JJ">direct</tok> <tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="VBG">regarding</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">alveolar</tok> <tok cat="NNS">macrophages</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NN">activation</tok> <tok cat="NNS">events</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1010100104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NNS">studies</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i1" role="SBJ"><tok cat="NN">rat</tok> <tok cat="NNS">lungs</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">depleted</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">alveolar</tok> <tok cat="NNS">macrophages</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">airway</tok> <tok cat="NN">instillation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">liposome-encapsulated</tok> <tok cat="NN">dichloromethylene</tok> <tok cat="NN">diphosphonate</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1010100105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">These</tok> <tok cat="NNS">procedures</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><cons cat="ADVP"><tok cat="RB">greatly</tok></cons> <tok cat="VBD">reduced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">retrievable</tok> <tok cat="JJ">alveolar</tok> <tok cat="NNS">macrophages</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">suppressed</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">lung</tok> <tok cat="NN">NF-kappaB</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inflammatory</tok> <tok cat="NN">model</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1010100106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="JJ">bronchoalveolar</tok> <tok cat="NN">lavage</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor-alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CXC</tok> <tok cat="NN">chemokine</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">macrophage</tok> <tok cat="JJ">inflammatory</tok> <tok cat="NN">protein-2</tok></cons><tok cat="COMMA">,</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">substantially</tok></cons> <tok cat="VBN">reduced</tok><cons cat="NP" ref="i3" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1010100107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">parallel</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="NN">upregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">lung</tok> <tok cat="JJ">vascular</tok> <tok cat="NN">adhesion</tok> <tok cat="NN">molecule</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">intercellular</tok> <tok cat="NN">adhesion</tok> <tok cat="NN">molecule-1</tok></cons><tok cat="COMMA">,</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">greatly</tok></cons> <tok cat="VBN">reduced</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">intrapulmonary</tok> <tok cat="NN">instillation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">phosphonate-containing</tok> <tok cat="NNS">liposomes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1010100108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">Neutrophil</tok> <tok cat="NN">accumulation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">development</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">lung</tok> <tok cat="NN">injury</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">substantially</tok></cons> <tok cat="VBN">diminished</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1010100109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Lung</tok> <tok cat="NN">instillation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">alveolar</tok> <tok cat="JJ">macrophage-depleted</tok> <tok cat="NNS">rats</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">restored</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">whole</tok> <tok cat="NN">lung</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1010100110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">inflammatory</tok> <tok cat="NN">model</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">initial</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">alveolar</tok> <tok cat="NNS">macrophages</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBG">ensuing</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">propagate</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">lung</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
1010103401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cyclooxygenase-2</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">4-trifluoromethyl</tok> <tok cat="NNS">derivatives</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">salicylate</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">triflusal</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="VBN">deacetylated</tok> <tok cat="NN">metabolite</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">2-hydroxy-4-trifluoromethylbenzoic</tok> <tok cat="NN">acid</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1010103402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">therapeutic</tok> <tok cat="NN">potential</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">drugs</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">block</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cyclooxygenase-2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">emphasized</tok><cons cat="NP" ref="i1" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1010103403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i15"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" id="i16" role="SBJ"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NN">4-trifluoromethyl</tok> <tok cat="NN">salicylate</tok> <tok cat="NNS">derivatives</tok></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">2-acetoxy-4-trifluoromethyl-benzoic</tok> <tok cat="NN">acid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">triflusal</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="VBN">deacetylated</tok> <tok cat="NN">metabolite</tok> <tok cat="JJ">2-hydroxy-4-trifluoromethylbenzoic</tok> <tok cat="NN">acid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HTB</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="RRB">]</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" ref="i16" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">aspirin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">sodium</tok> <tok cat="NN">salicylate</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">cyclooxygenase-2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">COX-2</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">inhibitors</tok></cons></cons><cons cat="ADVP" ref="i15" null="T"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">bacterial</tok> <tok cat="JJ">lipopolysaccharide-activated</tok></cons> <tok cat="JJ">human</tok> <tok cat="NN">blood</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">triflusal</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">aspirin</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HTB</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">sodium</tok> <tok cat="NN">salicylate</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="JJ">COX-2-mediated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">prostaglandin</tok> <tok cat="NN">E2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PGE2</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">production</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IC50</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><cons cat="QP"><tok cat="CD">0.16</tok><tok cat="COMMA">,</tok> <tok cat="CD">0.18</tok><tok cat="COMMA">,</tok> <tok cat="CD">0.39</tok><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <tok cat="JJR">&gt;</tok><tok cat="CD">10</tok></cons> <tok cat="NN">mM</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@81@@oe@16-12-2010
1010103404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="RB">only</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">triflusal</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">aspirin</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="VBN">purified</tok> <tok cat="NN">COX-2</tok> <tok cat="NN">enzyme</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
1010103405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">test</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">apparent</tok> <tok cat="NN">discrepancy</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i3" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">realized</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HTB</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">triflusal</tok></cons></cons> <tok cat="LRB">(</tok><tok cat="CC">but</tok> <cons cat="NP" syn="COOD"><tok cat="CC">neither</tok> <cons cat="NP"><tok cat="NN">aspirin</tok></cons> <tok cat="CC">nor</tok> <cons cat="NP"><tok cat="NN">salicylate</tok></cons></cons><tok cat="RRB">)</tok></cons> <cons cat="VP"><tok cat="VBD">produced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">concentration-dependent</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">COX-2</tok> <tok cat="NN">protein</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="JJ">human</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1010103406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">observation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">further</tok></cons> <tok cat="VBN">confirmed</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">rat</tok> <tok cat="NN">air</tok> <tok cat="NN">pouch</tok> <tok cat="NN">model</tok></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHPP" id="i5"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">aspirin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">triflusal</tok></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="NN">PGE2</tok> <tok cat="NN">production</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">ID50</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><cons cat="QP"><tok cat="CD">18.9</tok> <tok cat="CC">and</tok> <tok cat="CD">11.4</tok></cons> <tok cat="NN">mg/kg</tok> <tok cat="NN">p.o.</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="RB">only</tok> <tok cat="JJ">triflusal-treated</tok> <tok cat="NNS">animals</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">COX-2</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons><cons cat="PP" ref="i5" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
1010103407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="JJ">different</tok> <tok cat="NN">behavior</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">part</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP" role="PRD"><tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok> <cons cat="S" ref="i6" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HTB</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">triflusal</tok></cons></cons></cons> <cons cat="S" id="i6"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">block</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappaB</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJR">higher</tok> <tok cat="NN">extent</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">aspirin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">sodium</tok> <tok cat="NN">salicylate</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
1010103408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">addition</tok> <tok cat="TO">to</tok> <cons cat="S"><cons cat="NP" ref="i7" id="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inhibiting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">COX-2</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="JJ">therapeutic</tok> <tok cat="NNS">concentrations</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i7" role="SBJ"><tok cat="NN">triflusal</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">block</tok> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">metabolite</tok> <tok cat="NN">HTB</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">new</tok> <tok cat="NN">enzyme</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <tok cat="RB">hence</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">resumption</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PGE2</tok> <tok cat="NN">synthesis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
1010103409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Triflusal</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HTB</tok></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">exert</tok> <cons cat="NP"><tok cat="JJ">beneficial</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">processes</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i9"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="ADJP"><tok cat="FW">de</tok> <tok cat="FW">novo</tok></cons> <tok cat="NN">COX-2</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP" ref="i9" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="CC">and</tok><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJR">broader</tok> <tok cat="NN">sense</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">pathological</tok> <tok cat="NNS">situations</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i11"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="NNS">genes</tok></cons> <cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappaB</tok> <tok cat="NN">control</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">up-regulated</tok><cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP" ref="i11" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
1010124901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Vitamin</tok> <tok cat="NN">D</tok> <tok cat="NN">receptor</tok> <tok cat="JJ">3'-untranslated</tok> <tok cat="NN">region</tok> <tok cat="NNS">polymorphisms</tok></cons><tok cat="COLON">:</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lack</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">mRNA</tok> <tok cat="NN">stability</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@16-12-2010
1010124902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="JJ">Allelic</tok> <tok cat="NN">variation</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">3'-end</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">vitamin</tok> <tok cat="NN">D</tok> <tok cat="NN">receptor</tok> <tok cat="LRB">(</tok><tok cat="NN">VDR</tok><tok cat="RRB">)</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">3-5-fold</tok> <tok cat="VBN">increased</tok></cons> <tok cat="NN">risk</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">developing</tok> <cons cat="NP"><tok cat="NN">prostate</tok> <tok cat="NN">cancer</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">differences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">bone</tok> <tok cat="NN">mineralization</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1010124903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="JJ">genetic</tok> <tok cat="NN">diversity</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">alter</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">VDR</tok> <tok cat="NN">protein</tok></cons> <cons cat="ADVP"><tok cat="RB">structurally</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <tok cat="RB">instead</tok> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">marker</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">other</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">nearby</tok></cons></cons> <tok cat="NNS">polymorphisms</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">influence</tok> <cons cat="NP"><tok cat="NN">message</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">stability</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">translation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1010124904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">work</tok></cons> <cons cat="VP"><tok cat="VBN">reported</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">here</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">instigated</tok> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">identify</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">additional</tok> <tok cat="NN">VDR</tok> <tok cat="NN">3'-UTR</tok> <tok cat="NNS">polymorphisms</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="NN">significance</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">then</tok></cons> <tok cat="VB">test</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="JJ">genetic</tok> <tok cat="NNS">variants</tok></cons> <cons cat="VP"><tok cat="VBP">alter</tok> <cons cat="NP"><tok cat="NN">message</tok> <tok cat="NN">stability</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1010124905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Initially</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="CD">four</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">novel</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="RB">frequently</tok> <tok cat="VBG">occurring</tok></cons></cons> <tok cat="NN">sequence</tok> <tok cat="NNS">variants</tok></cons> <cons cat="SBAR" ref="i6" null="ICH"></cons></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="SBAR" id="i6"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">associated</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">common</tok> <tok cat="NNS">haplotypes</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" id="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">described</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1010124906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="DT">These</tok> <tok cat="JJ">common</tok> <tok cat="NN">sequence</tok> <tok cat="NNS">variants</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok> <tok cat="JJ">message-destabilizing</tok> <tok cat="NNS">elements</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i11"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">mapped</tok> <cons cat="NP" ref="i11" null="T"></cons><cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">3'-UTR</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">vitamin</tok> <tok cat="NN">D</tok> <tok cat="NN">receptor</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1010124907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="NN">VDR</tok> <tok cat="NN">3'-UTR</tok> <tok cat="NNS">haplotypes</tok></cons> <cons cat="VP"><tok cat="VBD">conferred</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">identical</tok> <tok cat="NN">half-life</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">heterologous</tok> <tok cat="NN">beta-globin</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">assay</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1010124908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">common</tok> <tok cat="NNS">polymorphisms</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">VDR</tok> <tok cat="NN">3'-UTR</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">do</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">influence</tok> <cons cat="NP"><tok cat="NN">message</tok> <tok cat="NN">stability</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1010160201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="LRB">[</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">Induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="FRAG"><cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">physiology</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">pharmacology</tok></cons></cons></cons></cons><tok cat="RRB">]</tok></cons></cons>@@@@1@14@@oe@16-12-2010
1010160202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">Glucocorticoids</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">physiological</tok> <tok cat="NNS">molecules</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" id="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">extensively</tok></cons> <tok cat="VBN">used</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">clinics</tok></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">anti-inflammatory</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">immunosuppressive</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">anti-tumoral</tok></cons></cons> <tok cat="NNS">agents</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1010160203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">Glucocorticoids</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="JJ">lymphoid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">key</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">physiology</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">thymic</tok> <tok cat="NN">selection</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1010160204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NNS">clinics</tok></cons></cons> <cons cat="NP" id="i3" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">molecules</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NNS">potencies</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inducing</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">malignant</tok> <tok cat="JJ">lymphoid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1010160205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="NNS">Glucocorticoids</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBG">mediating</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">intracellular</tok> <tok cat="NN">receptor</tok></cons> <cons cat="VP"><tok cat="VBG">belonging</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">steroid</tok> <tok cat="NN">receptor</tok> <tok cat="NN">superfamily</tok></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1010160206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Once</tok> <cons cat="S"><cons cat="NP" ref="i4" id="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">activated</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i4" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">GR</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">mediate</tok> <cons cat="NP"><tok cat="PRPP">his</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">direct</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DNA</tok></cons></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">protein/protein</tok> <tok cat="NNS">interactions</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1010160207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="VBG">Depending</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">type</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">fall</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">roughly</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">categories</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <tok cat="LQT">"</tok><cons cat="NP"><tok cat="NN">death</tok> <tok cat="NNS">genes</tok></cons><tok cat="RQT">"</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">activated</tok> <tok cat="NN">GR</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">c-jun</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">repression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">survival</tok> <tok cat="NNS">factors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">AP-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">c-Myc</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@48@@oe@16-12-2010
1010160208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">case</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">thymic</tok> <tok cat="NN">selection</tok></cons></cons></cons></cons> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="RBR">more</tok> <tok cat="JJ">subtle</tok></cons> <cons cat="PP"><tok cat="VBG">depending</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">mutual</tok> <tok cat="NN">repression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Nur77</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">GR</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1010262801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IkappaBalpha</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
1010262802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="VBG">According</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">current</tok> <tok cat="NNS">models</tok></cons></cons></cons> <cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NN">capacity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">I</tok><tok cat="LRB">(</tok><tok cat="NN">kappa</tok><tok cat="RRB">)</tok><tok cat="NN">B</tok><tok cat="LRB">(</tok><tok cat="NN">alpha</tok><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">would</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">retention</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Rel/NF-kappaB</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytosol</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1010262803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i2" role="SBJ"><tok cat="NN">I</tok><tok cat="LRB">(</tok><tok cat="NN">kappa</tok><tok cat="RRB">)</tok><tok cat="NN">B</tok><tok cat="LRB">(</tok><tok cat="NN">alpha</tok><tok cat="RRB">)</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i2" id="i3" null="NONE"></cons><cons cat="UCP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i5"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" id="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">overexpressed</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">transfection</tok></cons></cons><cons cat="ADVP" ref="i5" null="T"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1010262804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">gain</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJR">better</tok> <tok cat="NN">insight</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">potential</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">I</tok><tok cat="LRB">(</tok><tok cat="NN">kappa</tok><tok cat="RRB">)</tok><tok cat="NN">B</tok><tok cat="LRB">(</tok><tok cat="NN">alpha</tok><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">physiological</tok> <tok cat="NN">context</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="NP" id="i6" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">analysed</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PBL</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
1010262805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">localization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">I</tok><tok cat="LRB">(</tok><tok cat="NN">kappa</tok><tok cat="RRB">)</tok><tok cat="NN">B</tok><tok cat="LRB">(</tok><tok cat="NN">alpha</tok><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">PBL</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">techniques</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Western</tok> <tok cat="NN">blot</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">indirect</tok> <tok cat="NN">immunofluorescence</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">electron</tok> <tok cat="NN">microscopy</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1010262806@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="JJ">Low</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">I</tok><tok cat="LRB">(</tok><tok cat="NN">kappa</tok><tok cat="RRB">)</tok><tok cat="NN">B</tok><tok cat="LRB">(</tok><tok cat="NN">alpha</tok><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBG">resting</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">a</tok> <tok cat="NN">superinduction</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">PMA</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1010262807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">pool</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">I</tok><tok cat="LRB">(</tok><tok cat="NN">kappa</tok><tok cat="RRB">)</tok><tok cat="NN">B</tok><tok cat="LRB">(</tok><tok cat="NN">alpha</tok><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJR">higher</tok> <tok cat="NN">stability</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><tok cat="JJ">cytosolic</tok> <tok cat="NN">I</tok><tok cat="LRB">(</tok><tok cat="NN">kappa</tok><tok cat="RRB">)</tok><tok cat="NN">B</tok><tok cat="LRB">(</tok><tok cat="NN">alpha</tok><tok cat="RRB">)</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">partially</tok> <tok cat="JJ">independent</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">resynthesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
1010262808@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="ADVP"><tok cat="RB">Unexpectedly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">I</tok><tok cat="LRB">(</tok><tok cat="NN">kappa</tok><tok cat="RRB">)</tok><tok cat="NN">B</tok><tok cat="LRB">(</tok><tok cat="NN">alpha</tok><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">DNA</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">phenomenon</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="PP" role="PRD"><tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IkappaBbeta</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">level</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
1010262809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="NN">Immunoprecipitation</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="VP"><tok cat="VBD">failed</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">demonstrate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">association</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">I</tok><tok cat="LRB">(</tok><tok cat="NN">kappa</tok><tok cat="RRB">)</tok><tok cat="NN">B</tok><tok cat="LRB">(</tok><tok cat="NN">alpha</tok><tok cat="RRB">)</tok> <tok cat="CC">and</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1010262810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="VBG">resting</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">PMA-activated</tok></cons></cons> <tok cat="JJ">human</tok> <tok cat="NN">PBL</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">I</tok><tok cat="LRB">(</tok><tok cat="NN">kappa</tok><tok cat="RRB">)</tok><tok cat="NN">B</tok><tok cat="LRB">(</tok><tok cat="NN">alpha</tok><tok cat="RRB">)</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP"><tok cat="RB">apparently</tok> <tok cat="JJ">inactive</tok></cons> <tok cat="NN">form</tok></cons> <cons cat="ADJP"><tok cat="JJ">unable</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">disrupt</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">DNA</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
1010264101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Modulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">progesterone-induced</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NNS">factors</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
1010264102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Rat</tok> <tok cat="NN">spleen</tok> <tok cat="CC">and</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">progesterone</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WPP">whose</tok> <tok cat="NN">concentration</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" id="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">increased</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">greatly</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">early</tok> <tok cat="NN">phase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">pregnancy</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1010264103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">After</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">progesterone</tok></cons></cons></cons></cons> <cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">receptors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="JJ">augmented</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="ADVP"><tok cat="CD">2-3</tok> <tok cat="NNS">times</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
1010264104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i4"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">cultured</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">presence</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">progesterone</tok></cons></cons> <cons cat="ADVP" ref="i4" null="T"></cons></cons></cons></cons></cons><cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBD">released</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">soluble</tok> <tok cat="NN">factor</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NNS">immunoreactions</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">MLR</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CRC</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NN">proliferation</tok></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">measured</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">thymidine</tok> <tok cat="NN">incorporation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">spleen-cell</tok> <tok cat="NN">culture</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1010264105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">factor</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">synthesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">anti-DNP</tok> <tok cat="NNS">antibodies</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">mouse</tok> <tok cat="NN">hybridoma</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">diminished</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">proportion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">cells</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">phase</tok> <tok cat="NN">S</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1010264106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">percentage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">asymmetric</tok> <tok cat="NNS">molecules</tok></cons> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">hybridoma</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">remained</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unaltered</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1010264107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">support</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">hypothesis</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">soluble</tok> <tok cat="NNS">factors</tok></cons> <cons cat="VP"><tok cat="VBN">released</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">rat</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">modulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mother</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">participate</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">protects</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">fetus</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="JJ">antipaternal</tok> <tok cat="NNS">antibodies</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1010279101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">PGG-glucan</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">soluble</tok> <tok cat="NN">beta-(1,3)-glucan</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">enhances</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">oxidative</tok> <tok cat="NN">burst</tok> <tok cat="NN">response</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">microbicidal</tok> <tok cat="NN">activity</tok></cons><tok cat="COMMA">,</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">NF-kappa</tok> <tok cat="JJ">B-like</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">PMN</tok></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">glycosphingolipid</tok> <tok cat="NN">beta-(1,3)-glucan</tok> <tok cat="NN">receptor</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@32@@oe@16-12-2010
1010279102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">PGG-Glucan</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">soluble</tok> <tok cat="JJ">beta-(1,6)-branched</tok> <tok cat="JJ">beta-(1,3)-linked</tok> <tok cat="NN">glucose</tok> <tok cat="NN">homopolymer</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">wall</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">yeast</tok> <tok cat="FW">Saccharomyces</tok> <tok cat="FW">cerevisiae</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">immunomodulator</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">enhances</tok> <cons cat="NP"><tok cat="NN">leukocyte</tok> <tok cat="JJ">anti-infective</tok> <tok cat="NN">activity</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">enhances</tok> <cons cat="NP"><cons cat="UCP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">myeloid</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">megakaryocyte</tok> <tok cat="NN">progenitor</tok></cons></cons> <tok cat="NN">proliferation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1010279103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Incubation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">whole</tok> <tok cat="NN">blood</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">PGG-Glucan</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <tok cat="VBD">enhanced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">oxidative</tok> <tok cat="NN">burst</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="RB">subsequently</tok> <tok cat="VBN">isolated</tok> <tok cat="NN">blood</tok> <tok cat="NNS">leukocytes</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADJP"><tok cat="JJ">soluble</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">particulate</tok></cons></cons> <tok cat="NNS">activators</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">dose-dependent</tok> <tok cat="NN">manner</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><tok cat="NN">leukocyte</tok> <tok cat="JJ">microbicidal</tok> <tok cat="NN">activity</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1010279104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">No</tok> <tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NN">cytokine</tok> <tok cat="NN">production</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">conditions</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1010279105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PGG-Glucan</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">NF-kappaB-like</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">purified</tok> <tok cat="JJ">human</tok> <tok cat="NNS">neutrophils</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1010279106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">3H-PGG-Glucan</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">leukocyte</tok> <tok cat="NNS">membranes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="UCP" syn="COOD" role="PRD"><cons cat="ADJP"><tok cat="JJ">specific</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">concentration-dependent</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">saturable</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NN">affinity</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Kd</tok></cons> <cons cat="NP" role="PRD"><cons cat="QP"><tok cat="RB">approximately</tok> <tok cat="CD">6</tok></cons> <tok cat="NN">nM</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1010279107@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">monoclonal</tok> <tok cat="NN">antibody</tok></cons> <cons cat="ADJP"><tok cat="JJ">specific</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">glycosphingolipid</tok> <tok cat="NN">lactosylceramide</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">NF-kappaB-like</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">PGG-Glucan</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">ligand</tok> <tok cat="NN">binding</tok> <tok cat="NNS">data</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><tok cat="NN">polysaccharide</tok> <tok cat="NN">specificity</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">suggested</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">PGG-Glucan</tok> <tok cat="NN">binding</tok> <tok cat="NN">moiety</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><tok cat="NN">lactosylceramide</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
1010279108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PGG-Glucan</tok></cons> <cons cat="VP"><tok cat="VBZ">enhances</tok> <cons cat="NP"><tok cat="NN">neutrophil</tok> <tok cat="JJ">anti-microbial</tok> <tok cat="NNS">functions</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">beta-glucan</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">neutrophils</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">glycosphingolipid</tok> <tok cat="NN">lactosylceramide</tok></cons> <cons cat="ADJP"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">surface</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1010305901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Cleavage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">SP1</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NNS">caspases</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">anti-IgM-induced</tok> <tok cat="NN">B-cell</tok> <tok cat="NN">apoptosis</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1010305902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Apoptosis</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">instrumental</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">processes</tok></cons> <cons cat="VP"><tok cat="VBG">generating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">diversity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">B-cell</tok> <tok cat="NN">repertoire</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1010305903@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="JJ">Autoreactive</tok> <tok cat="NNS">B-cells</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">eliminated</tok> <cons cat="NP" ref="i1" id="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">anti-IgM</tok> <tok cat="NN">crosslinking</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">encountering</tok> <cons cat="NP"><tok cat="NNS">self-antigens</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="JJ">precise</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">still</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">not</tok> <tok cat="RB">well</tok></cons> <tok cat="VBN">understood</tok><cons cat="NP" ref="i3" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1010305904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cleavage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">SP1</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">Burkitt</tok> <tok cat="NN">lymphoma</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">BL60</tok></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">anti-IgM-induced</tok> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1010305905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Western</tok> <tok cat="NN">blot</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NN">cleavage</tok> <tok cat="NNS">products</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="QP"><tok cat="RB">approximately</tok> <tok cat="CD">68</tok> <tok cat="NN">kDa</tok> <tok cat="CC">and</tok> <tok cat="CD">45</tok></cons> <tok cat="NN">kDa</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1010305906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="NN">Cleavage</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">completely</tok></cons> <tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">zDEVD-fmk</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="ADJP"><tok cat="JJ">specific</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">caspase</tok> <tok cat="JJ">3-like</tok> <tok cat="NNS">proteases</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1010305907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="FW">In-vitro</tok> <tok cat="NN">cleavage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">recombinant</tok> <tok cat="NN">SP1</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">recombinant</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">caspase</tok> <tok cat="CD">3</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CPP32</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">caspase</tok> <tok cat="CD">7</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Mch</tok> <tok cat="CD">3</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">similar</tok> <tok cat="NN">cleavage</tok> <tok cat="NNS">products</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1010305908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Recombinant</tok> <tok cat="NN">caspase</tok> <tok cat="CD">6</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Mch</tok> <tok cat="CD">2</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">primarily</tok></cons> <cons cat="VP"><tok cat="VBZ">generates</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">68-kDa</tok> <tok cat="NN">cleavage</tok> <tok cat="NN">product</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="SBAR" adv="TMP"><tok cat="IN">after</tok> <cons cat="S"><cons cat="ADJP"><cons cat="NP"><cons cat="NP"><tok cat="NN">calcium</tok> <tok cat="NN">ionophore</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CaI</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="JJ">induced</tok></cons> <tok cat="NN">B-cell</tok> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1010305909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">caspase</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ICE</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">cleave</tok> <cons cat="NP"><tok cat="NN">SP1</tok></cons> <cons cat="ADVP"><tok cat="IN">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1010305910@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">time</tok> <tok cat="NN">course</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">SP1</tok> <tok cat="NN">cleavage</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">anti-IgM-induced</tok> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">paralleled</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">caspase</tok> <tok cat="NN">activity</tok></cons></cons> <cons cat="VP"><tok cat="VBN">measured</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">DEVD-p-nitroanilide</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">DEVD-pNA</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">cleavage</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1010305911@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">DNA</tok> <tok cat="NN">band-shift</tok> <tok cat="NNS">assays</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">intensity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="JJ">full</tok> <tok cat="NN">length</tok></cons> <tok cat="NN">SP1/DNA</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">intensity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJR">smaller</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">one</tok> <tok cat="NN">SP1</tok> <tok cat="NN">cleavage</tok> <tok cat="NN">product</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1010305912@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="NP"><tok cat="NN">Edman</tok> <tok cat="NN">sequencing</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">identify</tok> <cons cat="NP" id="i8"><tok cat="DT">a</tok> <tok cat="NN">caspase</tok> <tok cat="CD">3</tok> <tok cat="NN">cleavage</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Asp584</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">D584AQPQAGR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">generating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">22-kDa</tok> <tok cat="JJ">C-terminal</tok> <tok cat="NN">SP1</tok> <tok cat="NN">protein</tok> <tok cat="NN">fragment</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="T"></cons><cons cat="ADVP" adv="TMP"><tok cat="RB">still</tok></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1010305913@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cleavage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">SP1</tok></cons></cons></cons> <cons cat="ADVP" syn="COOD"><cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">underlining</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">central</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">caspase</tok> <tok cat="JJ">3-like</tok> <tok cat="NNS">proteases</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">process</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">anti-IgM-induced</tok> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1018781201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Spi-C</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="NN">Ets</tok> <tok cat="NN">protein</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" id="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">temporally</tok></cons> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NN">development</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1018781202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="DT">A</tok> <tok cat="JJ">novel</tok> <tok cat="NN">Ets</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">yeast</tok> <tok cat="JJ">one-hybrid</tok> <tok cat="NN">screening</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cDNA</tok> <tok cat="NN">library</tok></cons> <cons cat="VP"><tok cat="VBN">made</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">lipopolysaccharide-stimulated</tok> <tok cat="NN">mouse</tok> <tok cat="JJ">splenic</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">SP6</tok> <tok cat="NN">kappa</tok> <tok cat="NN">promoter</tok> <tok cat="NN">kappaY</tok> <tok cat="NN">element</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">bait</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1018781203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">novel</tok> <tok cat="NNS">Ets</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="ADVP"><tok cat="RBS">most</tok> <tok cat="RB">closely</tok></cons> <tok cat="JJ">related</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PU.1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Spi-B</tok></cons></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">Ets</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons> <cons cat="VP"><tok cat="VBN">named</tok> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NN">Spi-C</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1018781204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">Spi-C</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">represent</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="NN">subgroup</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Ets</tok> <tok cat="NN">protein</tok> <tok cat="NN">family</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">differed</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Spi-B</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">PU.1</tok></cons></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">helix</tok> <tok cat="CD">1</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Ets</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1018781205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">Spi-C</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">encoded</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">single-copy</tok> <tok cat="NN">gene</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" id="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">mapped</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="CD">10</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">region</tok> <tok cat="NN">C</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1018781206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Spi-C</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">interacted</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">DNA</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">similarly</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">PU.1</tok></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">judged</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">methylation</tok> <tok cat="NN">interference</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="UCP" syn="COOD"><cons cat="ADJP"><tok cat="NN">band-shift</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">site</tok> <tok cat="NN">selection</tok></cons></cons> <tok cat="NN">analysis</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">kappaY</tok> <tok cat="NN">element</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">co-transfection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HeLa</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
1018781207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="NN">Spi-C</tok> <tok cat="NN">RNA</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="NN">B</tok> <tok cat="NNS">lymphocytes</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJR">lower</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1018781208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="UCP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">pre-B</tok> <tok cat="NN">cell</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">plasma</tok> <tok cat="NN">cell</tok></cons></cons> <tok cat="NNS">lines</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">Spi-C-negative</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Spi-C</tok></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">molecule</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">specific</tok> <tok cat="NN">phase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="JJ">lymphoid</tok> <tok cat="NN">development</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1019193401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="LRB">[</tok><cons cat="NP"><tok cat="NNS">Corticoids</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">allergy</tok></cons><tok cat="RRB">]</tok></cons></cons>@@@@1@5@@oe@16-12-2010
1019193402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="NN">Inflammation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">constantly</tok></cons> <tok cat="VBN">observed</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">allergic</tok> <tok cat="NNS">reactions</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@16-12-2010
1019193403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">Corticosteroids</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="RBS">most</tok> <tok cat="JJ">effective</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">preventing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">late</tok> <tok cat="NN">phase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">allergic</tok> <tok cat="NN">reaction</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
1019193404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">action</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">glucocorticosteroids</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="ADJP"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">cytoplasm</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1019193405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i3"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" id="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">activated</tok><cons cat="NP" ref="i5" null="NONE"></cons><cons cat="ADVP" ref="i3" null="T"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i4" role="SBJ"><tok cat="NN">glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">form</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">dimer</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">DNA</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">migration</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1019193406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Interaction</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">DNA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">rate</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">induction</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">repression</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1019193407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">Glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">interact</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NNS">factors</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">AP-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">activator</tok> <tok cat="NN">protein-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappa</tok> <tok cat="NN">B</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1019193408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Through</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">actions</tok></cons></cons> <cons cat="NP" id="i7" role="SBJ"><tok cat="NNS">glucocorticosteroids</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">susceptible</tok> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">modify</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">functions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">allergic</tok> <tok cat="JJ">inflammatory</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1019193409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="PRP">They</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">particular</tok></cons></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJS">most</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">pro-inflammatory</tok> <tok cat="NNS">functions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">eosinophils</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1019238601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">human</tok> <tok cat="NN">IFNGR1</tok> <tok cat="JJ">small</tok> <tok cat="NN">deletion</tok> <tok cat="NN">hotspot</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">dominant</tok> <tok cat="NN">susceptibility</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">mycobacterial</tok> <tok cat="NN">infection</tok></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VB">see</tok> <cons cat="NP"><tok cat="NNS">comments</tok></cons></cons></cons><tok cat="RRB">]</tok></cons></cons>@@@@1@17@@oe@16-12-2010
1019238602@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">immunogenetic</tok> <tok cat="NN">basis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">severe</tok> <tok cat="NNS">infections</tok></cons> <cons cat="VP"><tok cat="VBN">caused</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="FW">bacille</tok> <tok cat="FW">Calmette-Guerin</tok> <tok cat="NN">vaccine</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">environmental</tok> <tok cat="NNS">mycobacteria</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">humans</tok> <tok cat="VBZ">remains</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="ADJP"><tok cat="RB">largely</tok> <tok cat="JJ">unknown</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1019238603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">describe</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">18</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NNS">generations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">12</tok> <tok cat="JJ">unrelated</tok> <tok cat="NNS">families</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WP">who</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">heterozygous</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="QP"><tok cat="CD">1</tok> <tok cat="TO">to</tok> <tok cat="CD">5</tok></cons> <tok cat="VBG">overlapping</tok> <tok cat="NN">IFNGR1</tok> <tok cat="NN">frameshift</tok> <tok cat="JJ">small</tok> <tok cat="NNS">deletions</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">wild-type</tok> <tok cat="NN">IFNGR1</tok> <tok cat="NN">allele</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1019238604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">There</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">12</tok> <tok cat="JJ">independent</tok> <tok cat="NN">mutation</tok> <tok cat="NNS">events</tok></cons> <cons cat="VP" ref="i2" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">at</tok> <tok cat="DT">a</tok> <tok cat="JJ">single</tok> <tok cat="NN">mutation</tok> <tok cat="NN">site</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP" id="i2"><tok cat="VBG">defining</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">small</tok> <tok cat="NN">deletion</tok> <tok cat="NN">hotspot</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1019238605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="VBG">Neighbouring</tok> <tok cat="NN">sequence</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBZ">favours</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">small</tok> <tok cat="NN">deletion</tok> <tok cat="NN">model</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">slipped</tok> <tok cat="NN">mispairing</tok> <tok cat="NNS">events</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">replication</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1019238606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">mutant</tok> <tok cat="NNS">alleles</tok></cons> <cons cat="VP"><tok cat="VBP">encode</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cell-surface</tok> <tok cat="NN">IFNgamma</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">lack</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">intra-cytoplasmic</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="WDT">which</tok><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">impaired</tok> <tok cat="NN">recycling</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="VBD">abrogated</tok> <tok cat="NN">signalling</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IFNgamma</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">exert</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">dominant-negative</tok> <tok cat="NN">effect</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
1019238607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">hotspot</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">IFNGR1</tok> <tok cat="JJ">small</tok> <tok cat="NNS">deletions</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="IN">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">confer</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">dominant</tok> <tok cat="NN">susceptibility</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">infections</tok></cons> <cons cat="VP"><tok cat="VBN">caused</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="RB">poorly</tok> <tok cat="JJ">virulent</tok> <tok cat="NNS">mycobacteria</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1019244601@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="JJ">Erythroid</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">differentially</tok></cons> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">erythropoietin</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">haemin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">delta-aminolaevulinic</tok> <tok cat="NN">acid</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">UT-7</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1019244602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="NN">Erythropoietin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Epo</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJR">later</tok> <tok cat="NNS">stages</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">erythropoiesis</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i2" id="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">acting</tok> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">promote</tok> <cons cat="NP"><tok cat="NN">cell</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">survival</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">proliferation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">remains</tok> <cons cat="S"><cons cat="NP" ref="i4" id="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">defined</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
1019244603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">UT-7</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">exhibits</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADJP"><tok cat="JJ">erythroid</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">megakaryocytic</tok></cons></cons> <tok cat="NNS">characteristics</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">differentiate</tok> <cons cat="PP"><tok cat="IN">along</tok> <cons cat="NP" id="i11"><tok cat="DT">the</tok> <tok cat="JJ">erythroid</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons></cons> <cons cat="PP" id="i12"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">Epo</tok></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="VP"><cons map="i11" cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">megakaryocytic</tok> <tok cat="NN">pathway</tok></cons> <cons map="i12" cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="JJ">myristic</tok> <tok cat="NN">acetate</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
1019244604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Epo</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">chemical</tok> <tok cat="NNS">inducers</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">delta-aminolaevulinic</tok> <tok cat="NN">acid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">delta-ALA</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">haemin</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">differentiation</tok> <tok cat="NN">capacity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">UT-7</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1019244605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Epo</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons> <cons cat="VP"><tok cat="VBD">promoted</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" adv="TMP"><tok cat="RB">relatively</tok> <tok cat="JJ">early</tok></cons> <tok cat="NNS">events</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NN">maturation</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">significant</tok> <tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">haemoglobin</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">production</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">morphology</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1019244606@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" syn="COOD" id="i7" role="SBJ"><cons cat="NP"><tok cat="NN">GATA-2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-myb</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">down-regulated</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">Epo</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="NN">GATA-2</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">further</tok></cons> <tok cat="VBN">down-modulated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">inducers</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1019244607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Conversely</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><tok cat="NN">SCL</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">up-regulated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">Epo</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">further</tok></cons> <tok cat="VBN">increased</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">haemin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">delta-ALA</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1019244608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Epo</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">proportion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glycophorin</tok> <tok cat="NN">A</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GPA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">up-regulated</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">beta-</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">gamma-globin</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NN">fold</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1019244609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">Both</tok> <cons cat="NP"><tok cat="NN">haemin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">delta-ALA</tok></cons></cons> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="FW">de</tok> <tok cat="FW">novo</tok></cons> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">alpha-globin</tok> <tok cat="NN">expression</tok></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="ADJP"><tok cat="VBG">enhancing</tok> <tok cat="JJ">Epo-induced</tok></cons> <tok cat="NN">beta-globin</tok> <tok cat="NN">expression</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">marked</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">haemoglobin</tok> <tok cat="NN">production</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1019244610@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="NN">haemoglobin</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">UT-7</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">limited</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">deficiency</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">erythroid-specific</tok> <tok cat="JJ">aminolaevulinic</tok> <tok cat="NN">acid</tok> <tok cat="NN">synthase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ALAS-E</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">activity</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">globin</tok> <tok cat="NN">synthesis</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">consequence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">immaturity</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">multipotential</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
1019402001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">polyclonal</tok> <tok cat="NN">etiology</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">palmar</tok> <tok cat="NNS">fibromatosis</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
1019402002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">X</tok> <tok cat="NN">chromosome</tok> <tok cat="NN">inactivation</tok> <tok cat="NNS">patterns</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">androgen</tok> <tok cat="NN">receptor</tok> <tok cat="NN">locus</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">evaluated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">clonality</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">microdissected</tok> <tok cat="JJ">lesional</tok> <tok cat="NN">tissue</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">leukocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">2</tok> <tok cat="NNS">women</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Dupuytren</tok><tok cat="POS">'s</tok></cons> <tok cat="NN">disease</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1019402003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">tissue</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">patients</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">generated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">polyclonal</tok> <tok cat="NN">pattern</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">X</tok> <tok cat="NN">chromosome</tok> <tok cat="NN">inactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">androgen</tok> <tok cat="NN">receptor</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1019402004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">finding</tok></cons> <cons cat="VP"><tok cat="VBZ">supports</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">polyclonal</tok> <tok cat="JJ">reactive</tok> <tok cat="NN">process</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">underlying</tok> <tok cat="NN">etiology</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">palmar</tok> <tok cat="NNS">fibromatosis</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1019418401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">Mycobacterium</tok> <tok cat="JJ">tuberculosis- induced</tok></cons> <tok cat="NN">interleukin-2</tok> <tok cat="NN">receptor</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NNS">phagocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
1019418402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="JJ">Soluble</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-2</tok> <tok cat="NN">receptor-alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-2Ralpha</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">reported</tok> <cons cat="S"><cons cat="NP" ref="i1" id="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">increased</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">sera</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">advanced</tok> <tok cat="NN">tuberculosis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBP">decline</tok> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">therapy</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">accordance</tok> <tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">improvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">radiologic</tok> <tok cat="NNS">findings</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
1019418403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2Ralpha</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">bronchoalveolar</tok> <tok cat="NN">lavage</tok> <tok cat="LRB">(</tok><tok cat="NN">BAL</tok><tok cat="RRB">)</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">active</tok> <tok cat="JJ">pulmonary</tok> <tok cat="NN">tuberculosis</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">evaluated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4" null="ZERO"></cons><cons cat="NP" role="SBJ"><tok cat="NN">Mycobacterium</tok> <tok cat="NN">tuberculosis</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP" ref="i4" null="T"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2Ralpha</tok></cons></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">THP-1</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NN">phagocyte</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
1019418404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">IL-2Ralpha</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">increased</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">BAL</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">involved</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">active</tok> <tok cat="JJ">pulmonary</tok> <tok cat="NN">tuberculosis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1019418405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">alpha-chain</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2Ralpha</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">monocytes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PBM</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">M.</tok> <tok cat="NN">tuberculosis</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">flow</tok> <tok cat="NN">cytometry</tok> <tok cat="NN">evaluation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1019418406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Northern</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">IL-2Ralpha</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">M.</tok> <tok cat="NN">tuberculosis</tok></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" id="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RBR">further</tok></cons> <tok cat="VBN">induced</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interferon-gamma</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1019418407@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">IL-2Ralpha</tok> <tok cat="NN">promoter</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="LRB">(</tok><tok cat="NN">NF-kappaB</tok><tok cat="RRB">)</tok> <tok cat="NN">site</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">transcriptionally</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">M.</tok> <tok cat="NN">tuberculosis</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">site</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">confer</tok> <cons cat="NP"><tok cat="NN">inducibility</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">heterologous</tok> <tok cat="NN">herpes</tok> <tok cat="NN">thymidine</tok> <tok cat="NN">kinase</tok> <tok cat="LRB">(</tok><tok cat="NN">TK</tok><tok cat="RRB">)</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">M.</tok> <tok cat="NN">tuberculosis</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
1019418408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">EMSAs</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">M.</tok> <tok cat="NN">tuberculosis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1019418409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><tok cat="NNS">antibodies</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">p50</tok> <tok cat="CC">and</tok> <tok cat="NN">p65</tok> <tok cat="NNS">subunits</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">EMSAs</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CC">both</tok> <tok cat="NN">p50</tok> <tok cat="CC">and</tok> <tok cat="NN">p65</tok> <tok cat="NNS">proteins</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RBR">further</tok></cons> <tok cat="VBN">demonstrated</tok><cons cat="NP" ref="i10" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1019418410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Functional</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2Ralpha</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NNS">phagocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">M.</tok> <tok cat="NN">tuberculosis</tok> <tok cat="NN">infection</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="JJ">immunomodulatory</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">host</tok> <tok cat="NN">response</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1019444301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">megakaryocytic</tok> <tok cat="NN">glycoprotein</tok> <tok cat="CD">IX</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">oncogenic</tok> <tok cat="NN">Ets</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">Fli-1</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1019444302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Glycoprotein</tok> <tok cat="LRB">(</tok><tok cat="NN">GP</tok><tok cat="RRB">)</tok> <tok cat="CD">IX</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">subunit</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">von</tok> <tok cat="NN">Willebrand</tok> <tok cat="NN">receptor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">GPIb-V-IX</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">mediates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">adhesion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">platelets</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">subendothelium</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">damaged</tok> <tok cat="NN">blood</tok> <tok cat="NNS">vessels</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1019444303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Previous</tok> <tok cat="NN">characterization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">GPIX</tok> <tok cat="NN">promoter</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">functional</tok> <tok cat="NN">Ets</tok> <tok cat="NN">site</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i5"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" id="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">disrupted</tok><cons cat="NP" ref="i4" null="NONE"></cons><cons cat="ADVP" ref="i5" null="T"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" ref="i2" id="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">reduced</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1019444304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Ets</tok> <tok cat="NN">protein</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">regulated</tok> <cons cat="NP"><tok cat="NN">GPIX</tok> <tok cat="NN">promoter</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unknown</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1019444305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">cotransfection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NN">GPIX</tok> <tok cat="NN">promoter/reporter</tok> <tok cat="NNS">constructs</tok></cons></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">293T</tok> <tok cat="NN">kidney</tok> <tok cat="NNS">fibroblasts</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">Fli-1</tok> <tok cat="NN">expression</tok> <tok cat="NN">vector</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">shows</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">oncogenic</tok> <tok cat="NN">protein</tok> <tok cat="NN">Fli-1</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">transactivate</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">GPIX</tok> <tok cat="NN">promoter</tok></cons> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i7"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">an</tok> <tok cat="JJ">intact</tok> <tok cat="NN">GPIX</tok> <tok cat="NN">Ets</tok> <tok cat="NN">site</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok></cons><cons cat="ADVP" ref="i7" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
1019444306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">Fli-1</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">GPIX</tok> <tok cat="NN">Ets</tok> <tok cat="NN">site</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">antibody</tok> <tok cat="NN">supershift</tok> <tok cat="NNS">experiments</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="JJ">human</tok> <tok cat="NN">erythroleukemia</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1019444307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Comparative</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="NN">Fli-1</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">transactivate</tok> <cons cat="NP" id="i15"><tok cat="DT">the</tok> <tok cat="NN">GPIbalpha</tok></cons> <cons cat="PP" id="i16" null="NONE"></cons></cons><tok cat="CC">and</tok><tok cat="COMMA">,</tok> <cons cat="VP"><cons map="i16" cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJR">lesser</tok> <tok cat="NN">extent</tok></cons></cons><tok cat="COMMA">,</tok> <cons map="i15" cat="NP"><tok cat="DT">the</tok> <tok cat="NN">GPIIb</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1019444308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Immunoblot</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><tok cat="NN">Fli-1</tok> <tok cat="NN">protein</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">lysates</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NNS">platelets</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
1019444309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Fli-1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">immunohistochemically</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">megakaryocytes</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">CD34(+)</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">megakaryocyte</tok> <tok cat="NN">differentiation</tok> <tok cat="CC">and</tok> <tok cat="NN">proliferation</tok> <tok cat="NN">factor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">thrombopoietin</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1019444310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="NN">Fli-1</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">likely</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><tok cat="JJ">lineage-specific</tok> <tok cat="NNS">genes</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">megakaryocytopoiesis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1019537901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Transient</tok> <tok cat="NN">pseudo-hypoaldosteronism</tok></cons> <cons cat="PP" adv="TMP"><tok cat="VBG">following</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">resection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ileum</tok></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">lymphocytic</tok> <tok cat="NN">aldosterone</tok> <tok cat="NNS">receptors</tok></cons></cons> <cons cat="PP"><tok cat="IN">outside</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">acute</tok> <tok cat="NN">phase</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
1019537902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Pseudo-hypoaldosteronism</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PHA</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="PP" role="PRD"><tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">mineralocorticoid</tok> <tok cat="NN">resistance</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">manifests</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">hyponatremia</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">hyperkalemia</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">plasma</tok> <tok cat="NN">aldosterone</tok> <tok cat="NNS">levels</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1019537903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="JJ">familial</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">secondary</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">abnormal</tok> <tok cat="JJ">renal</tok> <tok cat="NN">sodium</tok> <tok cat="NN">handling</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1019537904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">case</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">54-year-old</tok> <tok cat="NN">woman</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">multifocal</tok> <tok cat="NN">cancer</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">colon</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WP">who</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">developed</tok> <cons cat="NP"><tok cat="NN">PHA</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">subtotal</tok> <tok cat="NN">colectomy</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">ileal</tok> <tok cat="NN">resection</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">jejunostomy</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1019537905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="PRP">She</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">6</tok> <tok cat="NN">g</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">salt</tok></cons></cons></cons></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">daily</tok></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">prevent</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">dehydration</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">she</tok></cons> <cons cat="VP"><tok cat="VBD">stopped</tok> <cons cat="NP" ref="i3" null="T"></cons><cons cat="NP" func="ADV"><tok cat="PRP">herself</tok></cons> <cons cat="PP"><tok cat="IN">because</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">reduced</tok> <tok cat="JJ">fecal</tok> <tok cat="NNS">losses</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1019537906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><cons cat="NP"><tok cat="CD">One</tok> <tok cat="NN">month</tok></cons> <tok cat="RB">later</tok></cons> <cons cat="NP" id="i11" role="SBJ"><tok cat="PRP">she</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">admitted</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">signs</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">adrenal</tok> <tok cat="NN">failure</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="ADVP"><tok cat="FW">i.e.</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">fatigue</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">severe</tok> <tok cat="NN">nausea</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">blood</tok> <tok cat="NN">pressure</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">80/60</tok> <tok cat="NN">mmHg</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">extracellular</tok> <tok cat="NN">dehydration</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">hyponatremia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">118</tok> <tok cat="NN">mmol/l</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">hyperkalemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">7.6</tok> <tok cat="NN">mmol/l</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="VBN">increased</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">blood</tok> <tok cat="NN">urea</tok> <tok cat="NN">nitrogen</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">BUN</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">200</tok> <tok cat="NN">mg/dl</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">creatininemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">2.5</tok> <tok cat="NN">mg/dl</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">decreased</tok> <tok cat="NN">plasma</tok> <tok cat="NNS">bicarbonates</tok> <tok cat="NN">level</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">HCO3-</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" role="PRD"><tok cat="CD">16</tok> <tok cat="NN">mmol/l</tok></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">N</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" role="PRD"><tok cat="CD">27-30</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@74@@oe@16-12-2010
1019537907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">plasma</tok> <tok cat="NN">cortisol</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">high</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">66</tok> <tok cat="NN">microg/100</tok> <tok cat="NN">ml</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="CD">10:00</tok> <tok cat="NN">h</tok></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">N</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" role="PRD"><tok cat="CD">8-15</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">ACTH</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">normal</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="CD">13</tok> <tok cat="NN">pg/ml</tok></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">N</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" role="PRD"><tok cat="CD">10-60</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">marked</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">plasma</tok> <tok cat="NN">renin</tok> <tok cat="NN">activity</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="JJR">&gt;</tok><tok cat="CD">37</tok></cons> <tok cat="NN">ng/ml/h</tok></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">N</tok> <tok cat="NN">supine</tok></cons> <cons cat="NP" role="PRD"><cons cat="QP"><tok cat="JJR">&lt;</tok><tok cat="CD">3</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">active</tok> <tok cat="NN">renin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="CD">869</tok> <tok cat="NN">pg/ml</tok></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">N</tok> <tok cat="NN">supine</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" role="PRD"><tok cat="CD">1.120</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">aldosterone</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="JJR">&gt;</tok><tok cat="CD">2000</tok></cons> <tok cat="NN">pg/ml</tok></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">N</tok> <tok cat="NN">supine</tok></cons> <cons cat="NP" role="PRD"><cons cat="QP"><tok cat="JJR">&lt;</tok><tok cat="CD">150</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">plasma</tok> <tok cat="NN">AVP</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="CD">20</tok> <tok cat="NN">pmol/l</tok></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">N</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" role="PRD"><tok cat="CD">0.5-2.5</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@88@@oe@16-12-2010
1019537908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">plasma</tok> <tok cat="NN">ANH</tok> <tok cat="NN">level</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><tok cat="CD">38</tok> <tok cat="NN">pmol/l</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">N</tok> <tok cat="NN">supine</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" role="PRD"><tok cat="CD">5-25</tok></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1019537909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">A</tok> <tok cat="JJ">urinary</tok> <tok cat="NN">steroidogram</tok></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">highly</tok> <tok cat="JJ">elevated</tok></cons> <tok cat="NN">tetrahydrocortisol</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">THF</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" role="PRD"><tok cat="CD">13.3</tok> <tok cat="NN">mg/24h</tok></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">N</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" role="PRD"><tok cat="CD">1.4+/-0.8</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">tetrahydrocortisone</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">THE</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" role="PRD"><tok cat="CD">3.16</tok> <tok cat="NN">mg/24h</tok></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">N</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" role="PRD"><cons cat="QP"><tok cat="CD">2.7+/-2.0</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons> <tok cat="NN">excretion</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">low</tok> <tok cat="NN">THE/THF</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="CD">0.24</tok></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">N</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" role="PRD"><tok cat="CD">1.87+/-0.36</tok></cons></cons></cons><tok cat="RRB">)</tok></cons> <cons cat="NP" ref="i16" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">alpha</tok> <tok cat="NN">THF/THF</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="CD">0.35</tok></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">N</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" role="PRD"><tok cat="CD">0.92+/-0.42</tok></cons></cons></cons><tok cat="RRB">)</tok></cons> <cons cat="NP" ref="i16" null="RNR"></cons></cons></cons><cons cat="NP" id="i16"><tok cat="NNS">ratios</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@58@@oe@16-12-2010
1019537910@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">mineralocorticoid</tok> <tok cat="NNS">receptors</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NNS">leukocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="PP" role="PRD"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="JJR">lower</tok> <tok cat="JJ">normal</tok></cons> <tok cat="NN">range</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">age</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">reduced</tok><cons cat="NP" ref="i4" null="NONE"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1019537911@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Small-bowel</tok> <tok cat="NN">resection</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">ileostomized</tok> <tok cat="NNS">patients</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">causes</tok> <cons cat="NP"><tok cat="JJ">excessive</tok> <tok cat="JJ">fecal</tok> <tok cat="NN">sodium</tok> <tok cat="NNS">losses</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">chronic</tok> <tok cat="NN">sodium</tok> <tok cat="NN">depletion</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">contraction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">plasma</tok> <tok cat="NN">volume</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">severe</tok> <tok cat="JJ">secondary</tok> <tok cat="NN">hyperaldosteronism</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1019537912@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Nevertheless</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">hyperaldosteronism</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">hyponatremia</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">hyperkalemia</tok></cons></cons> <cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PHA</tok></cons> <cons cat="ADJP"><tok cat="JJ">related</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">major</tok> <tok cat="NN">importance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">colon</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">absorption</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">sodium</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1019537913@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">conclusion</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">case</tok> <tok cat="NN">report</tok></cons> <cons cat="VP"><tok cat="VBZ">emphasizes</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="LST"><tok cat="LS">1</tok><tok cat="RRB">)</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">possibility</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">syndrome</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">acquired</tok> <tok cat="NN">PHA</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">severe</tok> <tok cat="NN">hyperkalemia</tok></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">resection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ileum</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">colon</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBG">responding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">oral</tok> <tok cat="NN">salt</tok> <tok cat="NN">supplementation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><cons cat="LST"><tok cat="LS">2</tok><tok cat="RRB">)</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">major</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">AVP</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">small</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">ANH</tok></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><cons cat="LST"><tok cat="LS">3</tok><tok cat="RRB">)</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">strong</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">urinary</tok> <tok cat="NN">THF</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">low</tok> <tok cat="NN">THE/THF</tok> <cons cat="NP" ref="i15" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">alpha</tok> <tok cat="NN">THF/THF</tok> <cons cat="NP" ref="i15" null="RNR"></cons></cons></cons><cons cat="NP" id="i15"><tok cat="NNS">ratios</tok></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><cons cat="LST"><tok cat="LS">4</tok><tok cat="RRB">)</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">normal</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">lymphocytic</tok> <tok cat="NN">mineralocorticoid</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PP"><tok cat="IN">outside</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">acute</tok> <tok cat="NN">episode</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@77@@oe@16-12-2010
1019542801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">antigen</tok> <tok cat="CD">2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EBNA2</tok></cons><tok cat="RRB">)</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NN">immortalization</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">synthesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">I</tok> <tok cat="NN">interferon</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Burkitt</tok><tok cat="POS">'s</tok> <tok cat="NN">lymphoma</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1019542802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">antigen</tok> <tok cat="CD">2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EBNA2</tok></cons><tok cat="RRB">)</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">transformation</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">interferes</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">I</tok> <tok cat="NNS">interferons</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IFN-alpha/beta</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1019542803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">conditionally</tok> <tok cat="VBN">expressed</tok></cons> <tok cat="NN">EBNA2</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">context</tok> <tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IFN</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Burkitt</tok><tok cat="POS">'s</tok> <tok cat="NN">lymphoma</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1019542804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">EBNA2</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">led</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><tok cat="DT">both</tok> <cons cat="ADJP"><tok cat="JJ">endogenous</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="VBN">transfected</tok></cons></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">IFN-stimulated</tok> <tok cat="NNS">genes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">ISGs</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">genes</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">contain</tok> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NNS">promoters</tok></cons></cons> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">interferon-stimulated</tok> <tok cat="NN">response</tok> <tok cat="NN">element</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ISRE</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">gamma</tok> <tok cat="NN">interferon</tok> <tok cat="NN">activation</tok> <tok cat="NN">site</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GAS</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@16-12-2010
1019542805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">search</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">ISGs</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">EBNA2-dependent</tok> <tok cat="NN">synthesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IFN-beta</tok> <tok cat="NN">mRNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="JJ">low</tok> <tok cat="NNS">levels</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">secretion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">low</tok> <tok cat="NNS">amounts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IFN</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1019542806@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">A</tok> <tok cat="VBN">transfected</tok> <tok cat="NN">IFN-beta</tok> <tok cat="NN">promoter</tok></cons> <cons cat="VP"><tok cat="VBD">responded</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">EBNA2</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">sequence</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">closely</tok></cons> <tok cat="VBG">resembling</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">RBP-Jkappa</tok> <tok cat="NN">binding</tok> <tok cat="NN">site</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">pinpointed</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potential</tok> <tok cat="NN">target</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">EBNA2</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1019542807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">EBNA2-dependent</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IFN-beta</tok> <tok cat="NN">promoter</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">occurred</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">EBV-negative</tok> <tok cat="NN">Burkitt</tok><tok cat="POS">'s</tok> <tok cat="NN">lymphoma</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="JJ">other</tok> <tok cat="NN">EBV</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IFN-beta</tok> <tok cat="NN">synthesis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1019628601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="IN">factor</tok> <tok cat="JJ">receptor-associated</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">lymphocyte</tok> <tok cat="NN">transformation</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">herpesvirus</tok> <tok cat="NN">Saimiri</tok> <tok cat="NN">STP</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1019628602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">STP</tok> <tok cat="NNS">oncoproteins</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">herpesvirus</tok> <tok cat="NN">saimiri</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HVS</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">subgroup</tok> <tok cat="NN">A</tok> <tok cat="NN">strain</tok> <tok cat="CD">11</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">subgroup</tok> <tok cat="NN">C</tok> <tok cat="NN">strain</tok> <tok cat="CD">488</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i1" id="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">stably</tok></cons> <tok cat="VBN">associated</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="JJ">receptor-associated</tok></cons> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TRAF</tok></cons><tok cat="RRB">)</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">2</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="CD">3</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
1019628603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Mutational</tok> <tok cat="NNS">analyses</tok></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">residues</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PXQXT/S</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">STP-A11</tok></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="ADJP"><tok cat="JJ">critical</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">TRAF</tok> <tok cat="NN">association</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1019628604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">somewhat</tok> <tok cat="JJ">divergent</tok></cons> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STP-C488</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">critical</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">TRAF</tok> <tok cat="NN">association</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1019628605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Mutational</tok> <tok cat="NN">analysis</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">STP-C488</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" id="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">correlated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">associate</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NNS">TRAFs</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1019628606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">HVS</tok> <tok cat="NN">STP-C488</tok> <tok cat="NN">P10</tok><tok cat="TO">--&gt;</tok><tok cat="NN">R</tok> <tok cat="NN">mutant</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">deficient</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T-lymphocyte</tok> <tok cat="NN">transformation</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">interleukin-2-independent</tok> <tok cat="NN">growth</tok></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">wild-type</tok> <tok cat="NN">phenotype</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">marmoset</tok> <tok cat="NN">T-lymphocyte</tok> <tok cat="NN">transformation</tok></cons></cons></cons> <cons cat="ADVP" syn="COOD"><cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1019628607@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">STP-C488</tok> <tok cat="NN">P10</tok><tok cat="TO">--&gt;</tok><tok cat="NN">R</tok> <tok cat="NN">mutant</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">defective</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Rat-1</tok> <tok cat="NN">fibroblast</tok> <tok cat="NN">transformation</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">fibroblast</tok> <tok cat="NN">cell</tok> <tok cat="NN">transformation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">TRAF2</tok> <tok cat="JJ">dominant-negative</tok> <tok cat="NN">mutant</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1019628608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">implicate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">TRAFs</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">STP-C488-mediated</tok> <tok cat="NN">transformation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">lymphocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">rodent</tok> <tok cat="NNS">fibroblasts</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1019628609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="JJ">Other</tok> <tok cat="NNS">factors</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">immortalization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">common</tok> <tok cat="NN">marmoset</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">critical</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transformation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">lymphocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">rodent</tok> <tok cat="NNS">fibroblasts</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1019773101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">Studies</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphatase</tok> <tok cat="NN">inhibitor</tok> <tok cat="NN">phenylarsine</tok> <tok cat="NN">oxide</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NFkappaB</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">aging</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">altered</tok> <tok cat="NN">IkappaB-alpha</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">degradation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@16-12-2010
1019773102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Nuclear</tok> <tok cat="NN">Factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NFkappaB</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">critical</tok> <tok cat="NN">regulator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">immune</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">inflammatory</tok></cons></cons> <tok cat="NNS">responses</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1019773103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">variety</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">stimuli</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons></cons><tok cat="COMMA">,</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">active</tok> <tok cat="NN">p65-50</tok> <tok cat="NN">heterodimer</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">albeit</tok> <cons cat="FRAG"><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJR">lower</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">elderly</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
1019773104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">pretreatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">PAO</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NFkappaB</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">TNF-alpha</tok> <tok cat="JJ">treated</tok></cons> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">PAO-sensitive</tok> <tok cat="NN">phosphatase</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NFkappaB</tok></cons></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">pathway</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
1019773105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">demonstrates</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">aging</tok></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">influence</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">sensitivity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">phosphatase</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1019773106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">DMP</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="RB">prior</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PAO</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TNF</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">abolishes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">PAO</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADJP"><tok cat="JJ">young</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">old</tok></cons></cons> <tok cat="NNS">donors</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">alike</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1019773107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">a</tok> <tok cat="NN">failure</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">degrade</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IkappaB-alpha</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cytosols</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">TNF-treated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">pretreated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">PAO</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="PP" syn="COOD" role="PRD"><cons cat="PP"><tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">interference</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IkappaB-alpha</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <tok cat="RB">not</tok> <cons cat="PP"><tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">proteasomal</tok> <tok cat="NN">degradation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
1019773108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">collectively</tok></cons> <tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="NN">PAO</tok></cons> <cons cat="VP"><tok cat="VBZ">interferes</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phosphorylation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">regulated</tok> <tok cat="NN">degradation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IkappaB-alpha</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">TNF</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="S"><cons cat="NP" ref="i2" id="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">affecting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">chymotryptic</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">proteasome</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="ADJP" role="PRD"><tok cat="JJ">independent</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">age</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
1020000701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">In</tok> <tok cat="FW">vivo</tok></cons> <tok cat="NN">modulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok> <tok cat="NN">mRNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">inhaled</tok> <tok cat="NN">fluticasone</tok> <tok cat="NN">propionate</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">bronchial</tok> <tok cat="NN">mucosa</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">blood</tok> <tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">subjects</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">mild</tok> <tok cat="NN">asthma</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1020000702@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">BACKGROUND</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">In</tok> <tok cat="FW">vivo</tok></cons> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">provides</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">means</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">modulating</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">sensitivity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">targeted</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@16-12-2010
1020000703@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">OBJECTIVE</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">sought</tok> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <tok cat="NN">modulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GR</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">fluticasone</tok> <tok cat="NN">propionate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">FP</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">subjects</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">mild</tok> <tok cat="NN">asthma</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1020000704@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">METHODS</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="CD">Ten</tok> <cons cat="ADJP"><tok cat="JJ">atopic</tok> <tok cat="JJ">asthmatic</tok></cons> <tok cat="NNS">subjects</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">FP</tok> <tok cat="CD">250</tok> <tok cat="NN">microg</tok></cons></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">twice</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">daily</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="CD">4</tok> <tok cat="NNS">weeks</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1020000705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">Before</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">after</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons></cons><cons cat="NP" id="i3"><tok cat="NN">treatment</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">patients</tok></cons> <cons cat="VP"><tok cat="VBD">underwent</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">fiberoptic</tok> <tok cat="NN">bronchoscopy</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">endobronchial</tok> <tok cat="NN">biopsy</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">sampling</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">venous</tok> <tok cat="NN">blood</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">measurements</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GR</tok> <tok cat="NN">mRNA</tok> <tok cat="NNS">levels</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1020000706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="DT">A</tok> <tok cat="NN">solution</tok> <tok cat="NN">hybridization</tok> <tok cat="NN">assay</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">quantitative</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GR</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
1020000707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">24-hour</tok> <tok cat="JJ">urinary</tok> <tok cat="NN">cortisol</tok> <tok cat="NN">excretion</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">adrenocorticotropic</tok> <tok cat="NN">hormone</tok> <tok cat="NN">test</tok></cons></cons> <cons cat="PP" adv="TMP"><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">before</tok> <cons cat="NP" ref="i6" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">after</tok> <cons cat="NP" ref="i6" null="RNR"></cons></cons></cons><cons cat="NP" id="i6"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">FP</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">performed</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1020000708@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">RESULTS</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">high</tok> <tok cat="JJ">interindividual</tok> <tok cat="NN">variation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">GR</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">seen</tok><cons cat="NP" ref="i7" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
1020000709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">detected</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">significant</tok> <tok cat="NN">reduction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">GR</tok> <tok cat="NN">mRNA</tok> <tok cat="NNS">levels</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">endobronchial</tok> <tok cat="NN">biopsy</tok> <tok cat="NNS">specimens</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">FP</tok> <tok cat="NN">treatment</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">36.6</tok> <tok cat="CC">+/-</tok> <tok cat="CD">23.1</tok> <tok cat="CC">and</tok> <tok cat="CD">25.0</tok> <tok cat="CC">+/-</tok> <tok cat="CD">10.9</tok></cons> <tok cat="NN">amol</tok> <tok cat="NN">GR</tok> <tok cat="NN">mRNA/microg</tok> <tok cat="NN">RNA</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons> <cons cat="NP" role="PRD"><tok cat="JJR">&lt;</tok><tok cat="CD">.01</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
1020000710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP"><cons cat="ADVP"><tok cat="RB">even</tok> <tok cat="RBR">more</tok></cons> <tok cat="JJ">striking</tok></cons> <tok cat="NN">downregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">GR</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">cognate</tok> <tok cat="NN">ligand</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">documented</tok> <cons cat="NP" ref="i8" id="i9" null="NONE"></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">30.3</tok> <tok cat="CC">+/-</tok> <tok cat="CD">26.5</tok> <tok cat="CC">and</tok> <tok cat="CD">8.8</tok> <tok cat="CC">+/-</tok> <tok cat="CD">5</tok></cons> <tok cat="NN">amol</tok> <tok cat="NN">GR</tok> <tok cat="NN">mRNA/microg</tok> <tok cat="NN">RNA</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons> <cons cat="NP" role="PRD"><tok cat="JJR">&lt;</tok><tok cat="CD">.001</tok></cons></cons></cons><tok cat="RRB">)</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">possibly</tok></cons> <cons cat="VP"><tok cat="VBG">reflecting</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">differences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">sensitivity</tok></cons></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="NNS">tissues</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@48@@oe@16-12-2010
1020000711@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">small</tok></cons> <tok cat="CC">but</tok> <cons cat="ADJP"><tok cat="JJ">significant</tok></cons></cons> <tok cat="NN">reduction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">24-hour</tok> <tok cat="JJ">urinary</tok> <tok cat="NN">cortisol</tok> <tok cat="NN">excretion</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">233</tok> <tok cat="CC">+/-</tok> <tok cat="CD">109</tok> <tok cat="CC">and</tok> <tok cat="CD">157</tok> <tok cat="CC">+/-</tok> <tok cat="CD">66</tok></cons> <tok cat="NN">nmol/L</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons> <cons cat="NP" role="PRD"><tok cat="JJR">&lt;</tok><tok cat="CD">.01</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">feedback</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">synthesis</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <tok cat="NNS">means</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">hypothalamic-pituitary-adrenal</tok> <tok cat="NN">axis</tok></cons></cons> <cons cat="SBAR"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">assessed</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">adrenocorticotropic</tok> <tok cat="NN">hormone</tok> <tok cat="NN">test</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">remained</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">normal</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">FP</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@57@@oe@16-12-2010
1020000712@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">CONCLUSION</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">confirm</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">potency</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">inhaled</tok> <tok cat="NN">corticosteroid</tok> <tok cat="NN">FP</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">considerable</tok> <tok cat="JJ">tissue-specific</tok> <tok cat="JJ">interindividual</tok> <tok cat="NN">variation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">GR</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
1020029401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">novel</tok> <tok cat="JJ">lipopolysaccharide-induced</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">alpha</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">molecular</tok> <tok cat="NN">cloning</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">sequencing</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">characterization</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">chromosomal</tok> <tok cat="NN">assignment</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1020029402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Lipopolysaccharide</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LPS</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potent</tok> <tok cat="NN">stimulator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">causing</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">secretion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="JJ">inflammatory</tok> <tok cat="NNS">mediators</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1020029403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Given</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">deleterious</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">host</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">postulated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="MD">must</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADVP"><tok cat="RB">tightly</tok></cons> <cons cat="VP"><tok cat="VBN">regulated</tok><cons cat="NP" ref="i1" null="NONE"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1020029404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">nature</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">s</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">control</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">humans</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">remains</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">obscure</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="NN">NF-kappaB</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">suggested</tok><cons cat="NP" ref="i3" null="NONE"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1020029405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRPP">Our</tok> <tok cat="JJ">previous</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBG">pertaining</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">macrophage</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">LPS</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">domain</tok></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="CD">-550</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="CD">-487</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">TNF-alpha</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBZ">lacks</tok> <cons cat="NP"><tok cat="DT">any</tok> <tok cat="JJ">known</tok> <tok cat="JJ">NF-kappaB-binding</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
1020029406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">DNA</tok> <tok cat="NN">fragment</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">isolate</tok> <cons cat="NP" ref="i6" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">purify</tok> <cons cat="NP" ref="i6" null="RNR"></cons></cons><cons cat="NP" id="i6"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">60-kDa</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">fragment</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">amino-terminal</tok> <tok cat="NN">sequence</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">design</tok> <cons cat="NP"><tok cat="JJ">degenerate</tok> <tok cat="NNS">probes</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">screen</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cDNA</tok> <tok cat="NN">library</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">THP-1</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
1020029407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">A</tok> <tok cat="JJ">novel</tok> <tok cat="NN">cDNA</tok> <tok cat="NN">clone</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">1.8</tok> <tok cat="NN">kb</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" ref="i8" null="NONE"></cons></cons><tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">fully</tok></cons> <cons cat="VP"><tok cat="VBN">sequenced</tok><cons cat="NP" ref="i8" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1020029408@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Characterization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">cDNA</tok> <tok cat="NN">clone</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">its</tok> <tok cat="NN">induction</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">LPS</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">THP-1</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok></cons> <cons cat="ADVP"><tok cat="RB">hence</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">name</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">LPS-induced</tok> <tok cat="NN">TNF-alpha</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LITAF</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1020029409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">LITAF</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">THP-1</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">reduction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NNS">transcripts</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1020029410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">expression</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">LITAF</tok> <tok cat="NN">mRNA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">predominantly</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">placenta</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">leukocytes</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">lymph</tok> <tok cat="NNS">nodes</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">spleen</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1020029411@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">chromosomal</tok> <tok cat="NN">localization</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="NN">fluorescence</tok> <tok cat="FW">in</tok> <tok cat="FW">situ</tok> <tok cat="NN">hybridization</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">LITAF</tok></cons> <cons cat="VP"><tok cat="VBD">mapped</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="NN">16p12-16p13.3</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1020029412@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Together</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">LITAF</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">TNF-alpha</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">proposes</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">new</tok> <tok cat="NN">mechanism</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">control</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1020189901@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">p38</tok> <tok cat="JJ">mitogen-activated</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons> <cons cat="VP"><tok cat="VBZ">mediates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">integration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TCR/CD28</tok> <tok cat="NN">costimulation</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">murine</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1020189902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Optimal</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">signals</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP" id="i9"><tok cat="CD">one</tok></cons> <cons cat="VP"><tok cat="VBN">generated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP" id="i1"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">TCR</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" id="i10"><tok cat="DT">another</tok></cons> <cons cat="VP"><cons cat="NP" ref="i10" null="NONE"></cons><cons map="i1" cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="NN">CD28</tok> <tok cat="JJ">costimulatory</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1020189903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">costimulation-induced</tok> <tok cat="JJ">mitogen-activated</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="LRB">(</tok><tok cat="NN">MAPK</tok><tok cat="RRB">)</tok> <tok cat="NN">activation</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">mouse</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1020189904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">contrast</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="VP"><tok cat="VBN">reported</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i2" role="SBJ"><cons cat="NP"><tok cat="NN">p38</tok> <tok cat="NN">MAPK</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <tok cat="RB">not</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Jun</tok> <tok cat="JJ">NH2-terminal</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">JNK</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">weakly</tok></cons> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="NN">anti-CD3</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">anti-CD28</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NNS">thymocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">splenic</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
1020189905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i3" role="SBJ"><tok cat="NN">p38</tok> <tok cat="NN">MAPK</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="ADVP" syn="COOD"><cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">synergistically</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="NN">CD3/CD28</tok> <tok cat="NN">coligation</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">PMA/Ca2+</tok> <tok cat="NN">ionophore</tok> <tok cat="NN">stimulation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">mimics</tok> <cons cat="NP"><tok cat="JJ">TCR-CD3/CD28-mediated</tok> <tok cat="NN">signaling</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1020189906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p38</tok> <tok cat="NN">MAPK</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">correlates</tok> <cons cat="ADVP"><tok cat="RB">closely</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">proliferation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1020189907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="NN">PMA-induced</tok> <tok cat="NN">JNK</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">Ca2+</tok> <tok cat="NN">ionophore</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1020189908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">proliferation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">CD3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CD3/CD28</tok> <tok cat="NN">ligation</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok> <cons cat="NP" ref="i7" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">ATF-2</tok> <cons cat="NP" ref="i7" null="RNR"></cons></cons><cons cat="NP" id="i7"><tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="DT">each</tok></cons> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="NN">p38</tok> <tok cat="NN">MAPK</tok> <tok cat="NN">inhibitor</tok> <tok cat="NN">SB203580</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
1020189909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="NN">p38</tok> <tok cat="NN">MAPK</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><cons cat="LST"><tok cat="LS">1</tok><tok cat="RRB">)</tok></cons> <tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">integration</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">costimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">mouse</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><cons cat="LST"><tok cat="LS">2</tok><tok cat="RRB">)</tok></cons> <tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok> <tok cat="NN">activation</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">augmentation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><cons cat="LST"><tok cat="LS">3</tok><tok cat="RRB">)</tok></cons> <tok cat="VBZ">regulates</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">enter</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">state</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="NN">unresponsiveness</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@55@@oe@16-12-2010
1020192901@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Engagement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">natural</tok> <tok cat="NN">cytotoxicity</tok> <tok cat="NNS">programs</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">regulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NKL</tok> <tok cat="JJ">human</tok> <tok cat="NN">NK</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1020192902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NK</tok> <tok cat="NN">cell</tok> <tok cat="NN">cytotoxicity</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">fast</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">efficient</tok></cons></cons> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">target</tok> <tok cat="NN">cell</tok> <tok cat="NN">lysis</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1020192903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">analysis</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><tok cat="JJ">multiplex</tok> <tok cat="NN">messenger</tok> <tok cat="NNS">assays</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i1" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">natural</tok> <tok cat="NN">cytotoxicity</tok></cons> <cons cat="VP"><tok cat="VBN">exerted</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">NKL</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">correlates</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">mRNA</tok> <tok cat="NN">accumulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="RB">very</tok> <tok cat="JJ">early</tok> <tok cat="NN">activator</tok> <tok cat="NN">protein</tok> <tok cat="LRB">(</tok><tok cat="NN">AP</tok><tok cat="RRB">)</tok><tok cat="CD">-1</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NNS">genes</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">JunB</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">FosB</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Fos</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@16-12-2010
1020192904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NNS">activities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Jun-Fos</tok> <tok cat="NNS">heterodimers</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">course</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">natural</tok> <tok cat="NN">cytotoxicity</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1020192905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Interaction</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">immunoglobulin-like</tok> <tok cat="NN">transcript-2/leukocyte</tok> <tok cat="JJ">Ig-like</tok> <tok cat="NN">receptor</tok> <tok cat="CD">1</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">NKL</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HLA-B27</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">target</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">impairment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NKL</tok> <tok cat="JJ">natural</tok> <tok cat="NN">cytotoxicity</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">correlates</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">absence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">JunB</tok><tok cat="COMMA">,</tok> <tok cat="NN">FosB</tok><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <tok cat="NN">c-Fos</tok> <tok cat="NN">transcription</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">absence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@48@@oe@16-12-2010
1020192906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">studies</tok></cons> <cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">despite</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">rapidity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">NK</tok> <tok cat="JJ">cell-mediated</tok></cons> <tok cat="NN">lysis</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i4" role="SBJ"><tok cat="NN">AP-1</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">early</tok> <tok cat="NN">stage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NK</tok> <tok cat="NN">cell</tok> <tok cat="JJ">cytolytic</tok> <tok cat="NNS">programs</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">engagement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NK</tok> <tok cat="NN">cell</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">I</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">impairs</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" adv="TMP"><tok cat="RB">very</tok> <tok cat="JJ">early</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@49@@oe@16-12-2010
1020198401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">functional</tok> <tok cat="NN">synergy</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-12</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP"><tok cat="NN">p38</tok> <tok cat="JJ">mitogen-activated</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">augmentation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NN">serine</tok> <tok cat="NN">phosphorylation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1020198402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">IL-12</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">stimulate</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">mitogen-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">CD3-activated</tok></cons></cons> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">proliferate</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VB">produce</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">kill</tok> <cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1020198403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">magnitude</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="JJ">functional</tok> <tok cat="NNS">responses</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">greatly</tok></cons> <tok cat="JJ">augmented</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i3"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-12</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i3" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1020198404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">largely</tok> <tok cat="JJ">unresponsive</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cytokines</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP"><tok cat="JJ">prior</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">small</tok> <tok cat="NN">subset</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">CD8+</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CD8+CD18bright</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <tok cat="VBN">activated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-12</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
1020198405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">report</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">functional</tok> <tok cat="NN">synergy</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-12</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">CD8+CD18bright</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">correlates</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">stress</tok> <tok cat="NNS">kinases</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p38</tok> <tok cat="JJ">mitogen-activated</tok> <tok cat="NN">protein</tok> <tok cat="LRB">(</tok><tok cat="NN">MAP</tok><tok cat="RRB">)</tok> <tok cat="NN">kinase</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">stress-activated</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="LRB">(</tok><tok cat="NN">SAPK</tok><tok cat="RRB">)</tok><tok cat="NN">/Jun</tok> <tok cat="JJ">N-terminal</tok> <tok cat="NN">kinase</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">extracellular</tok> <tok cat="JJ">signal-regulated</tok> <tok cat="NNS">kinases</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@55@@oe@16-12-2010
1020198406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">functional</tok> <tok cat="NN">synergy</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-12</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">prominent</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">STAT1</tok> <tok cat="CC">and</tok> <tok cat="NN">STAT3</tok> <tok cat="NN">serine</tok> <tok cat="NN">phosphorylation</tok></cons></cons> <cons cat="PP"><tok cat="IN">over</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-12</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1020198407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i7" role="SBJ"><tok cat="NN">STAT</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="JJ">augmented</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">over</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="VP"><tok cat="VBN">seen</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">either</tok> <tok cat="NN">cytokine</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1020198408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">specific</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p38</tok> <tok cat="NN">MAP</tok> <tok cat="NN">kinase</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><cons cat="ADVP"><tok cat="RB">completely</tok></cons> <tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">serine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">STAT3</tok></cons></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-12</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">abrogates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">functional</tok> <tok cat="NN">synergy</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-12</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">affecting</tok> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
1020198409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">p38</tok> <tok cat="NN">MAP</tok> <tok cat="NN">kinase</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NN">serine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-12</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1020198410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">optimal</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-12</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">depend</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">p38</tok> <tok cat="NN">MAP</tok> <tok cat="NN">kinase</tok> <tok cat="CC">and</tok> <tok cat="NN">Janus</tok> <tok cat="NN">kinase/STAT</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1020202401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Human</tok> <tok cat="NN">cytomegalovirus</tok> <tok cat="VBG">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><tok cat="JJ">immunoregulatory</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
1020202402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">continue</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">our</tok> <tok cat="NN">investigation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cellular</tok> <tok cat="NNS">events</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">occur</tok> <cons cat="PP" adv="TMP"><tok cat="VBG">following</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">CMV</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HCMV</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">infection</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i2" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">focused</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP" adv="TMP"><tok cat="VBG">following</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1020202403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">First</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">viral</tok> <tok cat="NN">binding</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">immunoregulatory</tok> <tok cat="NNS">genes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-1beta</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">A20</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">NF-kappaB-p105/p50</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IkappaBalpha</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">unactivated</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="VBG">neutralizing</tok> <tok cat="NNS">Abs</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">major</tok> <tok cat="NN">HCMV</tok> <tok cat="NNS">glycoproteins</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">gB</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">UL55</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gH</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">UL75</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@53@@oe@16-12-2010
1020202404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Next</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="JJ">viral</tok> <tok cat="NNS">ligands</tok></cons> <cons cat="ADVP"><tok cat="RB">directly</tok></cons> <cons cat="VP"><tok cat="VBD">up-regulated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">monocyte</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">appropriate</tok> <tok cat="JJ">cellular</tok> <tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1020202405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">then</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">if</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HCMV</tok> <tok cat="NN">binding</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">translation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">secretion</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1020202406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">HCMV</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">production</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">release</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-1beta</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1020202407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Because</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="VBN">induced</tok> <tok cat="NN">gene</tok> <tok cat="NNS">products</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">promoter</tok> <tok cat="NNS">regions</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">next</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">up-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NNS">levels</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1020202408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">fact</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i3" role="SBJ"><tok cat="NN">NF-kappaB</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">translocated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="VBG">following</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">binding</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="VBN">purified</tok> <tok cat="JJ">viral</tok> <tok cat="NN">ligand</tok> <tok cat="NN">binding</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1020202409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">IkappaBalpha</tok> <tok cat="NNS">levels</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">correlated</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">translocation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1020202410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Lastly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">p38</tok> <tok cat="NN">kinase</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBD">played</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">central</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">IL-1beta</tok> <tok cat="NN">production</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok></cons> <tok cat="VBN">up-regulated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP" syn="COOD" adv="TMP"><tok cat="VBG">following</tok> <cons cat="NP"><tok cat="NN">infection</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1020202411@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">support</tok> <cons cat="NP"><tok cat="PRPP">our</tok> <tok cat="NN">hypothesis</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HCMV</tok></cons> <cons cat="VP"><tok cat="VBZ">initiates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok> <tok cat="NN">pathway</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">monocyte</tok> <tok cat="NN">activation</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">pinpoints</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potential</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i6"><tok cat="RB">whereby</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">HCMV</tok> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">result</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">profound</tok> <tok cat="NN">pathogenesis</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">especially</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">chronic</tok> <tok cat="JJ">inflammatory-type</tok> <tok cat="NNS">conditions</tok></cons></cons><cons cat="ADVP" ref="i6" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
1020202701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Signal</tok> <tok cat="NN">transduction</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="VBG">following</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">Chlamydia</tok> <tok cat="FW">pneumoniae</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
1020202702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Chlamydia</tok> <tok cat="FW">pneumoniae</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="JJ">respiratory</tok> <tok cat="NN">pathogen</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@16-12-2010
1020202703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Recently</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i1" role="SBJ"><tok cat="PRPP">its</tok> <tok cat="NN">presence</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">atherosclerotic</tok> <tok cat="NNS">lesions</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
1020202704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">characterized</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">C.</tok> <tok cat="JJ">pneumoniae-mediated</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="VBN">enhanced</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NN">adhesion</tok> <tok cat="NNS">molecules</tok></cons> <cons cat="VP"><tok cat="VBN">followed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">subsequent</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">rolling</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">adhesion</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">transmigration</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">leukocytes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">monocytes</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNS">granulocytes</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
1020202705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">effects</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">mAbs</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><cons cat="NP"><cons cat="UCP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">endothelial</tok></cons> <tok cat="CC">and/or</tok> <cons cat="NP"><tok cat="NN">leukocyte</tok></cons></cons> <tok cat="NN">adhesion</tok> <tok cat="NNS">molecules</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">beta1</tok> <tok cat="CC">and</tok> <tok cat="NN">beta2</tok> <tok cat="NNS">integrins</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1020202706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="ADVP"><tok cat="RB">Additionally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok> <tok cat="NNS">pathways</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="FW">C.</tok> <tok cat="JJ">pneumoniae-infected</tok> <tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok><cons cat="NP" ref="i3" null="NONE"></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">up-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">phosphorylated</tok> <tok cat="NN">p42/p44</tok> <tok cat="JJ">mitogen-activated</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation/translocation</tok></cons></cons> <cons cat="VP"><tok cat="VBD">occurred</tok> <cons cat="PP" adv="TMP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="CD">10-15</tok> <tok cat="NN">min</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
1020202707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i4" role="SBJ"><cons cat="NP"><tok cat="VBN">Increased</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">surface</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">E-selectin</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">ICAM-1</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">VCAM-1</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">noted</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="NNS">hours</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1020202708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="FW">C.</tok> <tok cat="FW">pneumoniae</tok></cons> <cons cat="VP"><tok cat="VBZ">triggers</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cascade</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">events</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">lead</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">endothelial</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">activation</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">inflammation</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">thrombosis</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="PP" adv="TMP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">turn</tok></cons></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">result</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" ref="i7" null="RNR"></cons></cons></cons></cons><tok cat="CC">or</tok> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">promote</tok> <cons cat="NP" ref="i7" null="RNR"></cons></cons></cons></cons><cons cat="NP" id="i7"><tok cat="NN">atherosclerosis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1020203401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="JJ">Extracellular-regulated</tok> <tok cat="NN">kinase</tok> <tok cat="CD">1/2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Jun</tok> <tok cat="JJ">N-terminal</tok> <tok cat="NN">kinase</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">NF-kappa</tok> <tok cat="JJ">B-dependent</tok></cons> <tok cat="NN">IL-6</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1020203402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">possible</tok> <tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">mitogen-activated</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">family</tok> <tok cat="NNS">members</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">extracellular-regulated</tok> <tok cat="NN">kinase</tok> <tok cat="CD">1/2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ERK1/2</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-Jun</tok> <tok cat="JJ">N-terminal</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">JNK</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="UCP" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">mediating</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-6</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">particular</tok></cons></cons> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">enhancing</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@48@@oe@16-12-2010
1020203403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">Freshly</tok> <tok cat="VBN">isolated</tok></cons> <tok cat="NNS">monocytes</tok></cons> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">protein</tok> <tok cat="NN">phosphatase</tok> <tok cat="NN">inhibitor</tok> <tok cat="JJ">okadaic</tok> <tok cat="NN">acid</tok></cons></cons></cons></cons> <cons cat="VP"><cons cat="VP"><tok cat="VBD">secreted</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-6</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">coincided</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">enhanced</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">activation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ERK1/2</tok> <tok cat="CC">and</tok> <tok cat="NN">JNK</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
1020203404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="ADJP"><tok cat="NN">ERK</tok> <tok cat="JJ">pathway-specific</tok></cons> <tok cat="NN">inhibitor</tok> <tok cat="NN">PD98059</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-6</tok> <tok cat="NN">secretion</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
1020203405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Transient</tok> <tok cat="NN">overexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">inactive</tok> <tok cat="NNS">mutants</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="NN">Raf-1</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">JNK1</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="DT">both</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">kappa</tok> <tok cat="JJ">B-dependent</tok></cons> <tok cat="NN">IL-6</tok> <tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1020203406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="NN">PD98059</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">Raf1/MEK1/ERK1/2</tok> <tok cat="NN">pathway</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons> <tok cat="CC">but</tok><tok cat="COMMA">,</tok> <tok cat="RB">rather</tok><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">acted</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
1020203407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Interestingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">NF-kappa</tok> <tok cat="JJ">B-mediated</tok></cons> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP" syn="COOD"><tok cat="DT">both</tok> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">context</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-6</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">own</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="JJ">serine</tok> <tok cat="NN">kinase</tok> <tok cat="NN">activity</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
1020203408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="ADJP"><tok cat="JJ">okadaic</tok> <tok cat="JJ">acid-induced</tok></cons> <tok cat="NN">IL-6</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP"><cons cat="ADVP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok></cons> <tok cat="RB">partly</tok></cons> <tok cat="VBN">mediated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="NN">ERK1/2</tok> <tok cat="CC">and</tok> <tok cat="NN">JNK</tok> <tok cat="JJ">pathway-dependent</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">capacity</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1020203409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">JNK</tok> <tok cat="NN">pathway</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">NF-kappa</tok> <tok cat="JJ">B-mediated</tok></cons> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">activation</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1020217801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Differential</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">interferon</tok> <tok cat="LRB">(</tok><tok cat="NN">IFN</tok><tok cat="RRB">)</tok><tok cat="JJ">-inducible</tok> <tok cat="NN">protein</tok> <tok cat="CD">10</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="VBG">following</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">monocyte</tok><tok cat="COMMA">,</tok> <tok cat="NN">macrophage</tok> <tok cat="NN">cell</tok> <tok cat="NN">lineage</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">related</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBN">bound</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IFN</tok> <tok cat="NN">stimulus</tok> <tok cat="NN">response</tok> <tok cat="NN">element</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">kappaB</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
1020217802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">chemokine</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP" adv="TMP"><tok cat="VBG">following</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">monocyte</tok><tok cat="COMMA">,</tok> <tok cat="NN">macrophage</tok> <tok cat="NN">cell</tok> <tok cat="NN">lineage</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1020217803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">human</tok> <tok cat="JJ">monoblastic</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">U937</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">differentiated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">12-myristate</tok> <tok cat="NN">13-acetate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PMA</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RA</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">vitamin</tok> <tok cat="NN">D3</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">VitD3</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
1020217804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interferon</tok> <tok cat="LRB">(</tok><tok cat="NN">IFN</tok><tok cat="RRB">)</tok><tok cat="JJ">-inducible</tok> <tok cat="NN">protein</tok> <tok cat="CD">10</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IP-10</tok></cons><tok cat="RRB">)</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">CXC</tok> <tok cat="NN">chemokine</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">markedly</tok></cons> <tok cat="VBN">augmented</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IFNgamma</tok> <tok cat="NN">treatment</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">PMA-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">RA-differentiated</tok></cons></cons> <tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok> <tok cat="RB">marginally</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">undifferentiated</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">VitD3-treated</tok></cons></cons> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
1020217805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i11" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">another</tok> <tok cat="JJ">inducible</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">monocyte</tok> <tok cat="NN">chemotactic</tok> <tok cat="NN">protein-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">CC</tok> <tok cat="NN">chemokine</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">FcRFgamma</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBN">bound</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">gamma</tok> <tok cat="NN">response</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP" syn="COOD"><cons cat="ADVP"><tok cat="RB">similarly</tok></cons> <tok cat="CC">or</tok> <cons cat="ADVP"><tok cat="RBR">less</tok> <tok cat="RB">abundantly</tok></cons></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">IFNgamma</tok> <tok cat="NN">treatment</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">PMA-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">RA-differentiated</tok></cons></cons> <tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="VBN">increased</tok> <tok cat="NN">IP-10</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">induction</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="PP" role="PRD"><tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">augmented</tok> <tok cat="NN">ability</tok> <cons cat="S" ref="i12" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="S" id="i12"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">respond</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IFNgamma</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@72@@oe@16-12-2010
1020217806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="VBN">Increased</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">IFNgamma-induced</tok> <tok cat="NN">IP-10</tok> <tok cat="NN">mRNA</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="VBG">following</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">largely</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">augmented</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">related</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">differentiation-dependent</tok> <tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBN">bound</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IFN</tok> <tok cat="NN">stimulus</tok> <tok cat="NN">response</tok> <tok cat="NN">element</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ISRE</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">kB</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IP-10</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">inducibility</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">IFNgamma</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@58@@oe@16-12-2010
1020293701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="JJ">Fludarabine-induced</tok> <tok cat="NN">immunosuppression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT1</tok> <tok cat="NN">signaling</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
1020293702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Fludarabine</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">nucleoside</tok> <tok cat="NN">analog</tok></cons> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">hematologic</tok> <tok cat="NNS">malignancies</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">severe</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">prolonged</tok></cons></cons> <tok cat="NN">immunosuppression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1020293703@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">incorporated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DNA</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBG">dividing</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">fludarabine</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potent</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">low</tok> <tok cat="NN">growth</tok> <tok cat="NN">fraction</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="RB">thus</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="MD">must</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">action</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1020293704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">STAT1</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" id="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">many</tok> <tok cat="JJ">lymphocyte-activating</tok> <tok cat="NNS">cytokines</tok></cons> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">interferons</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">cell-mediated</tok> <tok cat="NN">immunity</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">absence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">prominent</tok> <tok cat="NNS">defects</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">control</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NNS">infections</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
1020293705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">fludarabine</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immunosuppressant</tok> <tok cat="NN">cyclosporine</tok> <tok cat="NN">A</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cytokine-induced</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT1</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">STAT1-dependent</tok> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="VBG">resting</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="VBN">activated</tok></cons></cons> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1020293706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Fludarabine</tok></cons> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">specific</tok> <tok cat="NN">depletion</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT1</tok> <tok cat="NN">protein</tok></cons> <tok cat="LRB">(</tok><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">mRNA</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">STATs</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1020293707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="DT">This</tok> <tok cat="NN">loss</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">seen</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">fludarabine</tok></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1020293708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Brief</tok> <tok cat="NN">exposure</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">fludarabine</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">led</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">sustained</tok> <tok cat="NN">loss</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT1</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">analogous</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">prolonged</tok> <tok cat="NN">period</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">immunosuppression</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">exposure</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">drug</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1020293709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">STAT1</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <tok cat="JJ">useful</tok> <tok cat="NN">target</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">development</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">new</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">immunosuppressive</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">antineoplastic</tok></cons></cons> <tok cat="NNS">agents</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1020357701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Suppressive</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">anti-inflammatory</tok> <tok cat="NNS">agents</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">endothelial</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">heat</tok> <tok cat="NN">shock</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1020357702@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">BACKGROUND</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Studies</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="PRPP">our</tok> <tok cat="NN">laboratory</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJS">earliest</tok> <tok cat="NNS">stages</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">atherosclerosis</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">autoimmune</tok> <tok cat="NN">reaction</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">heat</tok> <tok cat="NN">shock</tok> <tok cat="NN">protein</tok> <tok cat="CD">60</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Hsp60</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@30@@oe@16-12-2010
1020357703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">interactions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">Hsp60-specific</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">arterial</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EC</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">require</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">Hsp60</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">certain</tok> <tok cat="NN">adhesion</tok> <tok cat="NNS">molecules</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="ADVP" adv="TMP"><tok cat="RB">simultaneously</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">EC</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">mechanical</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">other</tok></cons></cons> <tok cat="NNS">types</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">stress</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
1020357704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Recently</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">suppression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">T</tok> <tok cat="JJ">cell-mediated</tok></cons> <tok cat="JJ">immune</tok> <tok cat="NNS">responses</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cyclosporin</tok> <tok cat="NN">A</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CyA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">enhanced</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">atherosclerotic</tok> <tok cat="NN">lesion</tok> <tok cat="NN">formation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">mice</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1020357705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i3" role="SBJ"><tok cat="NN">aspirin</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">lower</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">risk</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">myocardial</tok> <tok cat="NN">infarction</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">men</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1020357706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="VBG">conflicting</tok> <tok cat="NNS">observations</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="PP" role="PRD"><tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">anti-inflammatory</tok> <tok cat="NNS">agents</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">adhesion</tok> <tok cat="NN">molecule</tok> <tok cat="CC">and</tok> <tok cat="NN">Hsp</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">EC</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1020357707@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">MATERIAL</tok></cons> <tok cat="CC">AND</tok> <cons cat="NP"><tok cat="NNS">METHODS</tok></cons></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">analyzed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CyA</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">aspirin</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">indomethacin</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">proliferation</tok></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">proliferation</tok> <tok cat="NN">assay</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1020357708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">explore</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">adhesion</tok> <tok cat="NNS">molecules</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">monocyte</tok> <tok cat="NN">chemoattractant</tok> <tok cat="NN">protein-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MCP-1</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Hsp60</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">umbilical</tok> <tok cat="NN">vein</tok> <tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">HUVECs</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="NN">Northern</tok> <tok cat="NN">blot</tok> <tok cat="NNS">analyses</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">used</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1020357709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">examine</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok> <tok cat="NN">status</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappaB</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">heat</tok> <tok cat="NN">shock</tok> <tok cat="NN">factor-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="ADJP"><tok cat="NN">HSF-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">performed</tok><cons cat="NP" ref="i6" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1020357710@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">RESULTS</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">With</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">exception</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">indomethacin</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="VBN">used</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">immunosuppressive</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">anti-inflammatory</tok></cons></cons> <tok cat="NNS">agents</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">proliferation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">influenza</tok> <tok cat="NN">virus</tok> <tok cat="NN">antigen</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">dose-dependent</tok> <tok cat="NN">manner</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1020357711@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Interestingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">CyA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">indomethacin</tok></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">suppress</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor-alpha</tok> <tok cat="LRB">(</tok><tok cat="NN">TNF-alpha</tok><tok cat="RRB">)</tok><tok cat="JJ">-induced</tok></cons> <tok cat="NN">adhesion</tok> <tok cat="NN">molecule</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NNS">HUVECs</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">aspirin</tok></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NN">effect</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1020357712@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">observations</tok></cons> <cons cat="VP"><tok cat="VBD">correlated</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">modulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">EC</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1020357713@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">All</tok> <tok cat="NNS">agents</tok></cons> <cons cat="VP"><tok cat="VBN">tested</tok> <cons cat="NP" null="NONE"></cons></cons></cons><cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Hsp60</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><cons cat="NP" func="ADV"><tok cat="CD">6</tok> <tok cat="NN">hr</tok></cons> <tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">application</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1020357714@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">aspirin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">indomethacin</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">CyA</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><tok cat="NN">Hsp70</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">HUVECs</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">correlated</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HSF-1</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1020357715@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">CONCLUSION</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">tested</tok> <tok cat="NNS">agents</tok></cons> <tok cat="LRB">(</tok><cons cat="PP"><tok cat="IN">except</tok> <cons cat="NP"><tok cat="NN">indomethacin</tok></cons></cons><tok cat="RRB">)</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="NNS">inhibitors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="NN">T</tok> <tok cat="JJ">cell-mediated</tok></cons> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" ref="i8" id="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">expected</tok><cons cat="NP" ref="i9" null="NONE"></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">aspirin</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">effective</tok> <tok cat="NN">suppressor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">adhesion</tok> <tok cat="NN">molecule</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">all</tok> <tok cat="CD">three</tok> <tok cat="NNS">agents</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><tok cat="NN">Hsp60</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">HUVECs</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@16-12-2010
1020357716@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">basis</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">notion</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">1</tok><tok cat="RRB">)</tok></cons> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">part</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">anti-atherogenic</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">aspirin</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="PP" role="PRD"><tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">prevention</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">adhesion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">sensitized</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">stressed</tok> <tok cat="NN">EC</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="SBAR"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">2</tok><tok cat="RRB">)</tok></cons> <tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">part</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">atherosclerosis-promoting</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CyA</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="PP" role="PRD"><tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">potential</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">inducer</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Hsp60</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">inability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">down-regulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">adhesion</tok> <tok cat="NN">molecule</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">EC</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <tok cat="CC">and</tok> <cons cat="SBAR"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">3</tok><tok cat="RRB">)</tok></cons> <tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">down-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MCP-1</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">aspirin</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">result</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">decreased</tok> <tok cat="NN">recruitment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">arterial</tok> <tok cat="NN">intima</tok></cons> <cons cat="PP"><tok cat="IN">beneath</tok> <cons cat="NP"><tok cat="JJ">stressed</tok> <tok cat="NN">EC</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@97@@oe@16-12-2010
1020648001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Amelioration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">rat</tok> <tok cat="NN">cerulein</tok> <tok cat="NN">pancreatitis</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">guamerin-derived</tok> <tok cat="NN">peptide</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="NN">elastase</tok> <tok cat="NN">inhibitor</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1020648002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="VBN">Increased</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NNS">proteases</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADJP"><tok cat="JJ">human</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">experimental</tok></cons></cons> <tok cat="NN">pancreatitis</tok></cons></cons></cons></cons><tok cat="COLON">;</tok></cons> <cons cat="S"><cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">information</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">protease</tok> <tok cat="NNS">inhibitors</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">disease</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">limited</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
1020648003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="NN">elastase</tok> <tok cat="NN">inhibitor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">guamerin-derived</tok> <tok cat="JJ">synthetic</tok> <tok cat="NN">peptide</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GDSP</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">improves</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">parameters</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cerulein-induced</tok> <tok cat="JJ">acute</tok> <tok cat="NN">pancreatitis</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">rat</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1020648004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GDSP</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">pancreatic</tok> <tok cat="NN">weight</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">serum</tok> <tok cat="NN">amylase</tok> <tok cat="CC">and</tok> <tok cat="NN">lipase</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">morphologic</tok> <tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pancreas</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">neutrophil</tok> <tok cat="NN">infiltration</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">KB</tok> <tok cat="LRB">(</tok><tok cat="NN">NF-KB</tok><tok cat="RRB">)</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">measured</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">rats</tok></cons> <cons cat="VP"><tok cat="VBN">infused</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">supramaximal</tok> <tok cat="NN">dose</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cerulein</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">5</tok> <tok cat="LRB">(</tok><tok cat="NN">g/kg/h</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="CD">6</tok> <tok cat="NN">h</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@49@@oe@16-12-2010
1020648005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GDSP</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">measured</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">superoxide</tok> <tok cat="NN">formation</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="VBN">activated</tok> <tok cat="JJ">human</tok> <tok cat="NNS">neutrophils</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1020648006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GDSP</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">another</tok> <tok cat="NN">elastase</tok> <tok cat="NN">inhibitor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">elastatinal</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1020648007@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">GDSP</tok></cons> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">edema</tok> <tok cat="NN">formation</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">neutrophil</tok> <tok cat="NN">infiltration</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">acinar</tok> <tok cat="NN">cell</tok> <tok cat="NN">damage</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">plasma</tok> <tok cat="NN">lipase</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP" role="SBJ"><tok cat="NN">amylase</tok></cons> <cons cat="VP"><tok cat="NNS">increases</tok> <cons cat="S" func="ADV"><cons cat="NP" id="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">caused</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">cerulein</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1020648008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">GDSP</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADVP"><tok cat="RB">completely</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">superoxide</tok> <tok cat="NN">formation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NNS">neutrophils</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="NN">N-formyl-methionine-leucine-phenyl-alanine</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">fMLP</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">12-O-tetradecanoylphorbol-13-acetate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TPA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1020648009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Elastatinal</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">some</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">GDSP</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" syn="COOD" role="PRD"><tok cat="JJR">less</tok> <cons cat="ADJP"><tok cat="JJ">potent</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">effective</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1020648010@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">beneficial</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GDSP</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="JJ">specific</tok> <tok cat="NN">elastase</tok> <tok cat="NN">inhibitor</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">development</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">rat</tok> <tok cat="NN">cerulein</tok> <tok cat="NN">pancreatitis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1020698301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Inhibition</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">signaling</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">mitogen-activated</tok> <tok cat="NN">protein</tok> <tok cat="JJ">kinase-targeted</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphatase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HePTP</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@16-12-2010
1020698302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="NN">Activation</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="SBAR"><cons cat="WHNP" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">produce</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="NN">counterbalance</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">protein-tyrosine</tok> <tok cat="NNS">kinases</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">protein-tyrosine</tok> <tok cat="NNS">phosphatases</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i10"><cons cat="NP"><tok cat="JJ">many</tok></cons> <cons cat="WHPP"><tok cat="IN">of</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons></cons> <cons cat="S"><cons cat="VP"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><tok cat="VBP">have</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">high</tok> <tok cat="NN">degree</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">substrate</tok> <tok cat="NN">specificity</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">because</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">physical</tok> <tok cat="NN">association</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NNS">targets</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@37@@oe@16-12-2010
1020698303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Overexpression</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="JJ">protein-tyrosine</tok> <tok cat="NN">phosphatase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HePTP</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">suppression</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" id="i2"><tok cat="NN">T</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="VP"><tok cat="VBN">measured</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">antigen</tok> <tok cat="JJ">receptor-induced</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">5'</tok> <tok cat="NN">promoter</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">interleukin-2</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
1020698304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">Efforts</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">pinpoint</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">exact</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <tok cat="NN">action</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">specificity</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HePTP</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">signaling</tok> <tok cat="NN">cascade</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HePTP</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">acts</tok> <cons cat="ADVP"><tok cat="RB">directly</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">mitogen-activated</tok> <tok cat="NN">protein</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MAP</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">kinases</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP" id="i3"><tok cat="NN">Erk1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" ref="i3" null="QSTN"></cons><tok cat="CD">2</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">consequently</tok></cons> <tok cat="VBZ">reduces</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">magnitude</tok> <cons cat="PP" ref="i4" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">duration</tok> <cons cat="PP" ref="i4" null="RNR"></cons></cons><cons cat="PP" id="i4"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">catalytic</tok> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">intact</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@48@@oe@16-12-2010
1020698305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <tok cat="NN">contrast</tok></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HePTP</tok></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">N-terminal</tok> <tok cat="NN">c-Jun</tok> <tok cat="NN">kinase</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NNS">events</tok> <cons cat="ADVP"><tok cat="RB">upstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">MAP</tok> <tok cat="NNS">kinases</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1020698306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">specificity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HePTP</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">correlated</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">physical</tok> <tok cat="NN">association</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <tok cat="PRPP">its</tok> <tok cat="JJ">noncatalytic</tok> <cons cat="NP"><tok cat="NN">N</tok> <tok cat="NN">terminus</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Erk</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">another</tok> <cons cat="NP"><tok cat="NN">MAP</tok> <tok cat="NN">kinase</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p38</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <tok cat="RB">not</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Jnk</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1020698307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">propose</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">HePTP</tok></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">negative</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">antigen</tok> <tok cat="NN">receptor</tok> <tok cat="NN">signaling</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><tok cat="NN">MAP</tok> <tok cat="NNS">kinases</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytosol</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">time</tok> <tok cat="NNS">points</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">before</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">activation-induced</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="JJ">dual-specific</tok> <tok cat="NN">MAP</tok> <tok cat="NN">kinase</tok> <tok cat="NNS">phosphatases</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
1020702301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Modulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">E2F</tok> <tok cat="NNS">complexes</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">G0</tok></cons> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">S</tok></cons></cons> <tok cat="NN">phase</tok> <tok cat="NN">transition</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">primary</tok> <tok cat="NNS">B-lymphocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1020702302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">pocket</tok> <tok cat="JJ">protein-E2F</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="NN">convergence</tok> <tok cat="NNS">points</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">cycle</tok> <tok cat="NN">signaling</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1020702303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">report</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">monitored</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons></cons><cons cat="NP" id="i1"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pocket</tok> <tok cat="JJ">protein-E2F</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">primary</tok> <tok cat="NNS">B-lymphocytes</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">12-myristate</tok> <tok cat="NN">13-acetate</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1020702304@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="ADJP" role="PRD"><tok cat="JJ">Consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">previous</tok> <tok cat="NNS">data</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="UCP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">human</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">mouse</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">fibroblasts</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">T-lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i2" role="SBJ"><cons cat="NP"><tok cat="NN">E2F4</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">DP1</tok></cons></cons> <cons cat="VP"><tok cat="VBP">form</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">predominant</tok> <tok cat="NN">E2F</tok> <tok cat="NNS">heterodimers</tok></cons> <cons cat="PP" adv="TMP"><cons cat="NP" func="ADV"><tok cat="CC">both</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">G0</tok> <tok cat="CC">and</tok> <tok cat="NN">G1</tok> <tok cat="NNS">phases</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">B-lymphocyte</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i3"><cons cat="NP"><tok cat="NN">E2F1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-3</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">first</tok></cons> <tok cat="VBN">detected</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">late</tok> <tok cat="NN">G1</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">their</tok> <tok cat="NN">expression</tok> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBP">increase</tok> <cons cat="PP"><tok cat="IN">towards</tok> <cons cat="NP"><tok cat="NN">S</tok> <tok cat="NN">phase</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@53@@oe@16-12-2010
1020702305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Intriguingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">major</tok> <tok cat="NN">E2F</tok> <tok cat="NN">complex</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">detected</tok> <cons cat="NP" ref="i5" null="T"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">quiescent</tok> <tok cat="JJ">human</tok> <tok cat="NNS">B-lymphocytes</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">consisted</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">pRB</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">E2F4</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">DP1</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1020702306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Though</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">DP1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-2</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">increase</tok> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i6"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">progress</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">G0</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">S</tok></cons></cons><cons cat="ADVP" ref="i6" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">proportion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DP1</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">DP2</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">remains</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">relatively</tok> <tok cat="JJ">constant</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1020702307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">predominant</tok> <tok cat="NN">E2F</tok> <tok cat="NNS">components</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">DP1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">E2F4</tok></cons></cons><tok cat="COMMA">,</tok></cons></cons></cons></cons></cons> <cons cat="SBAR" func="ADV" adv="TMP"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">B-lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBD">progressed</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">G0</tok></cons></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">G1</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1020702308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">This</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">mobility</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">attributable</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">dephosphorylation</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">lambda</tok> <tok cat="NN">phosphatase</tok> <tok cat="NN">treatment</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">convert</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJR">slower</tok> <tok cat="JJ">migrating</tok> <tok cat="NNS">forms</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">corresponding</tok> <tok cat="JJR">faster</tok> <tok cat="NN">mobility</tok> <tok cat="NNS">forms</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1020702309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">further</tok></cons> <tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">change</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">phosphorylation</tok> <tok cat="NN">status</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">correlates</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1020702310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">This</tok> <tok cat="NN">modulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">dephosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">DP1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">E2F4</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">help</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">explain</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">lack</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <tok cat="NN">DNA</tok> <tok cat="NN">footprinting</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">late</tok> <tok cat="NN">G1</tok> <tok cat="CC">and</tok> <tok cat="NN">S</tok> <tok cat="NNS">phases</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NNS">promoters</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">negatively</tok></cons> <tok cat="VBN">regulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="NN">E2F</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">controlling</tok> <cons cat="NP"><tok cat="NN">E2F</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transition</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">quiescence</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">proliferation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@57@@oe@16-12-2010
1020706101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><cons cat="ADJP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Retinoid</tok> <tok cat="NN">X</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RXR</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="JJ">agonist-induced</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">dominant-negative</tok> <tok cat="JJ">RXR-retinoic</tok> <tok cat="NN">acid</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha403</tok> <tok cat="NNS">heterodimers</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">developmentally</tok></cons> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1020706102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">multiple</tok> <tok cat="JJ">biologic</tok> <tok cat="NNS">activities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">RAR</tok> <tok cat="CC">and</tok> <tok cat="NN">retinoid</tok> <tok cat="NN">X</tok> <tok cat="NN">receptor</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RXR</tok></cons><tok cat="RRB">)</tok></cons> <tok cat="JJ">nuclear</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">interact</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">DNA</tok> <tok cat="NN">target</tok> <tok cat="VBZ">sequences</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NNS">heterodimers</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RXR-RAR</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">homodimers</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RXR-RXR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
1020706103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">RA</tok> <tok cat="NN">receptor</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">critical</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">important</tok> <tok cat="NNS">aspects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">hematopoiesis</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">since</tok> <cons cat="S"><cons cat="S" role="SBJ"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">transducing</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="RB">COOH-terminally</tok> <tok cat="VBN">truncated</tok> <tok cat="NN">RARalpha</tok></cons> <cons cat="VP"><tok cat="VBG">exhibiting</tok> <cons cat="NP"><tok cat="JJ">dominant-negative</tok> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RARalpha403</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">mouse</tok> <tok cat="NN">bone</tok> <tok cat="NN">marrow</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">generates</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NN">growth</tok> <tok cat="JJ">factor-dependent</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="VP"><tok cat="VBN">frozen</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">multipotent</tok> <tok cat="NN">progenitor</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EML</tok></cons><tok cat="RRB">)</tok></cons> <tok cat="CC">or</tok> <tok cat="VBN">committed</tok> <tok cat="JJ">promyelocyte</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MPRO</tok></cons><tok cat="RRB">)</tok></cons> <tok cat="NNS">stages</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@16-12-2010
1020706104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Nevertheless</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="RB">relatively</tok> <tok cat="JJ">high</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">pharmacological</tok></cons></cons> <tok cat="NNS">concentrations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">RA</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="QP"><tok cat="CD">1</tok> <tok cat="TO">to</tok> <tok cat="CD">10</tok></cons> <tok cat="CC">&amp;</tok><tok cat="NN">mgr</tok><tok cat="COLON">;</tok><tok cat="NN">M</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">overcome</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NN">differentiation</tok> <tok cat="NNS">blocks</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">terminal</tok> <tok cat="JJ">granulocytic</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">MPRO</tok> <tok cat="NNS">promyelocytes</tok></cons></cons></cons></cons></cons> <cons cat="SBAR" func="ADV" adv="TMP"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">potentiating</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-3</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-3</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="JJ">-induced</tok></cons> <tok cat="NN">commitment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">EML</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">granulocyte/monocyte</tok> <tok cat="NN">lineage</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@16-12-2010
1020706105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">utilized</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">RXR-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">RAR-specific</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">agonists</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">antagonists</tok></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="SBAR"><cons cat="WHADVP" id="i6"><tok cat="WRB">how</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">RA</tok></cons> <cons cat="VP"><tok cat="VBZ">overcomes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">dominant-negative</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">truncated</tok> <tok cat="NN">RARalpha</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="JJ">different</tok> <tok cat="JJ">myeloid</tok> <tok cat="JJ">developmental</tok> <tok cat="NNS">stages</tok></cons></cons></cons><cons cat="ADVP" ref="i6" null="T"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1020706106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Unexpectedly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">RXR-specific</tok><cons cat="NP" ref="i15" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">rather</tok> <tok cat="IN">than</tok></cons> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">RAR-specific</tok><cons cat="NP" ref="i15" null="RNR"></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="NP" id="i15"><tok cat="NN">agonist</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">terminal</tok> <tok cat="JJ">granulocytic</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MPRO</tok> <tok cat="NNS">promyelocytes</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">response</tok> <tok cat="NNS">elements</tok></cons> <cons cat="VP"><tok cat="VBG">corresponding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">RAR-RXR</tok> <tok cat="NNS">heterodimers</tok></cons> <cons cat="CONJP"><tok cat="RB">rather</tok> <tok cat="IN">than</tok></cons> <cons cat="NP"><tok cat="NN">RXR-RXR</tok> <tok cat="NNS">homodimers</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
1020706107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">RXR</tok> <tok cat="NN">agonist</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">RAR-specific</tok> <tok cat="NNS">antagonists</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">extensive</tok> <tok cat="NN">cross-talk</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">partners</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">RXR -RARalpha403</tok> <tok cat="NN">heterodimer</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1020706108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="RBR">more</tok> <tok cat="JJ">immature</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">multipotent</tok></cons></cons> <tok cat="NN">EML</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="JJ">RXR-specific</tok> <tok cat="NN">agonist</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">inactive</tok></cons> <cons cat="PP" syn="COOD"><tok cat="CC">either</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">potentiating</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">IL-3-mediated</tok> <tok cat="NN">commitment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">EML</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">granulocyte</tok> <tok cat="NN">lineage</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">transactivating</tok> <cons cat="NP"><tok cat="NN">RAR-RXR</tok> <tok cat="NN">response</tok> <tok cat="NNS">elements</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
1020706109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">RA- triggered</tok> <tok cat="NN">GALdbd-RARalpha</tok> <tok cat="NN">hybrid</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">indicates</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">multipotent</tok> <tok cat="NN">EML</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">harbor</tok> <cons cat="NP"><tok cat="JJ">substantial</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">hormone</tok> <tok cat="NN">receptor</tok> <tok cat="NN">coactivator</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1020706110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">histone</tok> <tok cat="NN">deacetylase</tok> <tok cat="LRB">(</tok><tok cat="NN">HDAC</tok><tok cat="RRB">)</tok> <tok cat="NN">inhibitor</tok> <tok cat="NN">trichostatin</tok> <tok cat="NN">A</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">readily</tok></cons> <tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">RXR-RAR</tok> <tok cat="NN">reporter</tok> <tok cat="NN">construct</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">multipotent</tok> <tok cat="NN">EML</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">committed</tok> <tok cat="NN">MPRO</tok> <tok cat="NNS">promyelocytes</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">differences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">HDAC-containing</tok> <tok cat="NN">repressor</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="CD">two</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="RB">closely</tok> <tok cat="JJ">related</tok></cons> <tok cat="CC">but</tok> <cons cat="ADJP"><tok cat="JJ">distinct</tok></cons></cons> <tok cat="JJ">hematopoietic</tok> <tok cat="NNS">lineages</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">account</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">differential</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">RXR-RARalpha403</tok> <tok cat="NNS">heterodimers</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i11"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="NP" ref="i11" null="T"></cons><cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">these</tok> <tok cat="JJ">different</tok> <tok cat="NNS">stages</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NN">development</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@67@@oe@16-12-2010
1020846101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Bcl-6</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">reactive</tok> <tok cat="JJ">follicular</tok> <tok cat="NN">hyperplasia</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">follicular</tok> <tok cat="NN">lymphoma</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">angioimmunoblastic</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">lymphoma</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">hyperplastic</tok> <tok cat="JJ">germinal</tok> <tok cat="NNS">centers</tok></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">heterogeneity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">intrafollicular</tok> <tok cat="NNS">T-cells</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">altered</tok> <tok cat="NN">distribution</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathogenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">angioimmunoblastic</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">lymphoma</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@35@@oe@16-12-2010
1020846102@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">BACKGROUND</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">Bcl-6</tok> <tok cat="NN">gene</tok> <tok cat="NN">product</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">phosphoprotein</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">independently</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Bcl-6</tok> <tok cat="NN">gene</tok> <tok cat="NN">rearrangement</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@16-12-2010
1020846103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">lymph</tok> <tok cat="NNS">nodes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Bcl-6</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="JJ">restricted</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">germinal</tok> <tok cat="NN">center</tok> <tok cat="LRB">(</tok><tok cat="NN">GC</tok><tok cat="RRB">)</tok> <tok cat="NNS">B-cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">10</tok><tok cat="NN">%</tok> <tok cat="TO">to</tok> <tok cat="CD">15</tok><tok cat="NN">%</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">CD3/CD4+</tok> <tok cat="JJ">intrafollicular</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1020846104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Interfollicular</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">negative</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">Bcl-6</tok> <tok cat="NN">protein</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">except</tok> <tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">rare</tok> <tok cat="JJ">CD3+/CD4+</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1020846105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Recently</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">reported</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cases</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">angioimmunoblastic</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">lymphoma</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AITL</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">hyperplastic</tok> <tok cat="NNS">GCs</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AITL/GC</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">borders</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">enlarged</tok> <tok cat="NNS">GCs</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">ill</tok> <tok cat="VBN">defined</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">features</tok></cons> <cons cat="ADJP"><tok cat="JJ">suggestive</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">outward</tok> <tok cat="NN">migration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GC</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">surrounding</tok> <tok cat="JJ">interfollicular</tok> <tok cat="NNS">zones</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@16-12-2010
1020846106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBD">prompted</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">study</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">follicular</tok> <tok cat="NNS">borders</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">Bcl-6</tok> <tok cat="NN">staining</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">reactive</tok> <tok cat="JJ">follicular</tok> <tok cat="NN">hyperplasias</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">follicular</tok> <tok cat="NNS">lymphomas</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">compare</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">AITL/GC</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1020846107@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">MATERIALS</tok></cons> <tok cat="CC">AND</tok> <cons cat="NP"><tok cat="NNS">METHODS</tok></cons></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="JJ">Formalin-fixed</tok> <tok cat="NN">paraffin</tok> <tok cat="NNS">sections</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">immunostaining</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Bcl-6</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1020846108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i5" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="CD">Six</tok> <tok cat="NNS">cases</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AITL/GC</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">12</tok> <tok cat="JJ">nonspecific</tok> <tok cat="JJ">reactive</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">follicular</tok> <tok cat="NN">hyperplasia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">FH</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">7</tok> <tok cat="NN">HIV</tok> <tok cat="NN">adenopathy</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">10</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">follicular</tok> <tok cat="NN">lymphoma</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">FL</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">8</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">typical</tok> <tok cat="NN">AITL</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="ADVP"><tok cat="FW">ie</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AITL</tok></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP"><tok cat="NN">GC</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">studied</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
1020846109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="JJ">Double</tok> <tok cat="NN">staining</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Bcl-6/CD20</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Bcl-6/CD3</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Bcl-6/CD57</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">performed</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">selected</tok> <tok cat="NNS">cases</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1020846110@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">RESULTS</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">FH</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">adenopathy</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">staining</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">Bcl-6</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">densely</tok> <tok cat="JJ">populated</tok></cons> <tok cat="NNS">GCs</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">well-defined</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">regular</tok></cons></cons> <tok cat="NN">GC</tok> <tok cat="NNS">borders</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Bcl-6+</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">rare</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">interfollicular</tok> <tok cat="NNS">areas</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1020846111@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="DT">An</tok> <tok cat="JJ">occasional</tok> <tok cat="NN">GC</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">ill-defined</tok> <tok cat="NN">border</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">invariably</tok></cons> <tok cat="VBN">surrounded</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">broad</tok> <tok cat="NN">mantle</tok> <tok cat="NN">zone</tok></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">indistinct</tok> <tok cat="NN">mantle</tok> <tok cat="NNS">zones</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">well-defined</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">regular</tok></cons></cons> <tok cat="NNS">borders</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1020846112@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">FL</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="NNS">follicles</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">densely</tok></cons> <tok cat="JJ">populated</tok><cons cat="NP" ref="i8" null="NONE"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">their</tok> <tok cat="NNS">borders</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">irregular</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">some</tok> <tok cat="JJ">Bcl-6+</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">interfollicular</tok> <tok cat="NNS">zones</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1020846113@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">AITL/GC</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">GCs</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="RBR">less</tok> <tok cat="JJ">dense</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">GC</tok> <tok cat="NNS">borders</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" syn="COOD" role="PRD"><cons cat="ADJP"><tok cat="RB">ill</tok> <tok cat="VBN">defined</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">irregular</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">interfollicular</tok> <tok cat="JJ">Bcl-6+</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">markedly</tok></cons> <tok cat="VBN">increased</tok><cons cat="NP" ref="i10" null="NONE"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1020846114@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Double</tok> <tok cat="NN">staining</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">these</tok> <tok cat="JJ">interfollicular</tok> <tok cat="JJ">Bcl-6+</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">AITL/GC</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="NP" role="PRD"><tok cat="JJ">Bcl6+/CD3+/CD20-/CD57-</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1020846115@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i11" role="SBJ"><tok cat="JJ">CD3+</tok> <tok cat="JJ">intrafollicular</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">depleted</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">AITL/GC</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">abundant</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">FH</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1020846116@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="JJ">Intrafollicular</tok> <tok cat="JJ">CD57+</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">stain</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">Bcl-6</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">depleted</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">AITL/GC</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1020846117@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">typical</tok> <tok cat="NN">AITL</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i13" role="SBJ"><tok cat="DT">some</tok> <tok cat="JJ">neoplastic</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">positive</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">Bcl-6</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i13" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">showing</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">variable</tok> <tok cat="NNS">degrees</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">staining</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1020846118@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">CONCLUSIONS</tok></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">1</tok><tok cat="RRB">)</tok></cons> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">GCs</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AITL/GC</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">differed</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">other</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">reactive</tok> <tok cat="JJ">follicular</tok> <tok cat="NN">hyperplasias</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">follicular</tok> <tok cat="NNS">lymphomas</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="VBG">staining</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">Bcl-6</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">useful</tok> <cons cat="S"><cons cat="NP" ref="i14" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">discern</tok> <cons cat="NP"><tok cat="PRP">them</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1020846119@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">2</tok><tok cat="RRB">)</tok></cons> <cons cat="NP" id="i15" role="SBJ"><cons cat="NP"><tok cat="JJ">Intrafollicular</tok> <tok cat="JJ">CD3+</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i17"><cons cat="NP"><tok cat="JJ">many</tok></cons> <cons cat="WHPP"><tok cat="IN">of</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons></cons> <cons cat="S"><cons cat="NP" ref="i17" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">positive</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">Bcl-6</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">markedly</tok></cons> <tok cat="VBN">depleted</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">AITL/GC</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="JJ">interfollicular</tok> <tok cat="JJ">Bcl-6+/CD3+</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">outward</tok> <tok cat="NN">migration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">intrafollicular</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">condition</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
1020846120@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">3</tok><tok cat="RRB">)</tok></cons> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Interfollicular</tok> <tok cat="JJ">Bcl-6+/CD3+</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">AITL/GC</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">too</tok> <tok cat="JJ">numerous</tok> <cons cat="SBAR"><cons cat="WHNP" id="i19" null="ZERO"></cons><cons cat="S"><cons cat="NP" ref="i19" id="i20" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">accounted</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" ref="i20" null="NONE"></cons></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">migration</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><tok cat="JJ">local</tok> <tok cat="NN">proliferation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1020846121@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">4</tok><tok cat="RRB">)</tok></cons> <cons cat="NP" role="SBJ"><tok cat="JJ">Intrafollicular</tok> <tok cat="JJ">CD57+</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">negative</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">Bcl-6</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">heterogeneity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">intrafollicular</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">population</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1020846122@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">5</tok><tok cat="RRB">)</tok></cons> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">Some</tok> <tok cat="JJ">neoplastic</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">AITL</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">stained</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">Bcl-6</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">up-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Bcl-6</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">tumor</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1020886701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Angiotensin</tok> <tok cat="CD">II</tok></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">proinflammatory</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappaB</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
1020886702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">renin-angiotensin</tok> <tok cat="NN">system</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathogenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">atherosclerosis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
1020886703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">common</tok> <tok cat="NN">feature</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="NNS">stages</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">atherosclerosis</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="NN">inflammation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">vessel</tok> <tok cat="NN">wall</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1020886704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappaB</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">participates</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJS">most</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">inflammation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1020886705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">study</tok></cons> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">angiotensin</tok> <tok cat="LRB">(</tok><tok cat="NN">ANG</tok><tok cat="RRB">)</tok> <tok cat="CD">II</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">major</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">component</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">atheroma</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1020886706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">ANG</tok> <tok cat="CD">II</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">like</tok> <cons cat="NP"><tok cat="NN">TNFalpha</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">rapid</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">Ficoll</tok> <tok cat="NN">density</tok> <tok cat="NN">gradient</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1020886707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">ANG</tok> <tok cat="CD">II</tok> <tok cat="NN">effect</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">angiotensin</tok> <tok cat="NN">AT1</tok> <tok cat="NN">receptor</tok> <tok cat="NN">antagonist</tok> <tok cat="NN">losartan</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1020886708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="NN">Specificity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ANG</tok> <tok cat="JJ">II-induced</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">ascertained</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">supershift</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">competition</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons><cons cat="NP" id="i3"><tok cat="NNS">experiments</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1020886709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">ANG</tok> <tok cat="CD">II</tok></cons> <cons cat="VP"><tok cat="VBD">stimulated</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <tok cat="RB">not</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NN">preparation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1020886710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Together</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok></cons> <cons cat="S" ref="i4" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">vasoactive</tok> <tok cat="NN">peptide</tok> <tok cat="NN">ANG</tok> <tok cat="CD">II</tok></cons></cons> <cons cat="S" id="i4"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1020886711@GENIA Treebank@formal@@1@S@<cons cat="NP"><tok cat="NN">Copyright</tok> <tok cat="CD">1999</tok> <tok cat="NNP">Academic</tok> <tok cat="NNP">Press</tok><tok cat="PERIOD">.</tok></cons>@@@@1@5@@oe@16-12-2010
1020903601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">SHP2-interacting</tok> <tok cat="JJ">transmembrane</tok> <tok cat="NN">adaptor</tok> <tok cat="NN">protein</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SIT</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="JJ">disulfide-linked</tok> <tok cat="NN">dimer</tok></cons> <cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1020903602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="JJ">low</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">weight</tok> <tok cat="JJ">transmembrane</tok> <tok cat="NN">adaptor</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">recruit</tok> <cons cat="NP"><tok cat="NN">src</tok> <tok cat="NN">homology</tok> <tok cat="LRB">(</tok><tok cat="NN">SH</tok><tok cat="RRB">)</tok><tok cat="CD">2</tok> <tok cat="JJ">domain-containing</tok> <tok cat="JJ">intracellular</tok> <tok cat="NNS">molecules</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">membrane</tok></cons></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="JJ">tyrosine-based</tok> <tok cat="JJ">signaling</tok> <tok cat="NNS">motifs</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1020903603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">describe</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="NN">molecule</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">group</tok></cons> <cons cat="VP"><tok cat="VBN">termed</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><cons cat="NP"><tok cat="NN">SIT</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SHP2</tok> <tok cat="NN">interacting</tok> <tok cat="JJ">transmembrane</tok> <tok cat="NN">adaptor</tok> <tok cat="NN">protein</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1020903604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">SIT</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">disulfide-linked</tok> <tok cat="JJ">homodimeric</tok> <tok cat="NN">glycoprotein</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" id="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1020903605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">After</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">src</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">possibly</tok></cons> <cons cat="NP"><tok cat="NN">syk</tok></cons></cons></cons></cons></cons> <cons cat="NP" role="SBJ"><tok cat="NN">protein</tok> <tok cat="NN">tyrosine</tok> <tok cat="NNS">kinases</tok> <tok cat="NN">SIT</tok></cons> <cons cat="VP"><tok cat="VBZ">recruits</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">SH2</tok> <tok cat="JJ">domain-containing</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphatase</tok> <tok cat="NN">SHP2</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">immunoreceptor</tok> <tok cat="JJ">tyrosine-based</tok> <tok cat="NN">inhibition</tok> <tok cat="NN">motif</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1020903606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Overexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SIT</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">downmodulates</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">receptor-</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">phytohemagglutinin-mediated</tok> <tok cat="NN">activation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="IN">of</tok> <tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-AT</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">interfering</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">signaling</tok> <tok cat="NNS">processes</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">probably</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">located</tok> <cons cat="ADVP"><tok cat="RB">upstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">phospholipase</tok> <tok cat="NN">C</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
1020903607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SHP2</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">SIT</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok> <tok cat="NN">induction</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">SIT</tok></cons> <cons cat="VP" syn="COOD"><cons cat="CONJP"><tok cat="RB">not</tok> <tok cat="RB">only</tok></cons> <cons cat="VP"><tok cat="VBZ">regulates</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok> <tok cat="NN">activity</tok></cons></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">controls</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">NF-AT</tok> <tok cat="JJ">unrelated</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="PP"><tok cat="VBG">involving</tok> <cons cat="NP"><tok cat="NN">SHP2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
1020904101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NN">GrpL</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">Grb2-related</tok> <tok cat="NN">adaptor</tok> <tok cat="NN">protein</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">interacts</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">SLP-76</tok></cons></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="IN">of</tok> <tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1020904102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Propagation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">signals</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">antigen</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TCR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">adaptor</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1020904103@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="NN">SH2</tok> <tok cat="JJ">domain-containing</tok> <tok cat="NN">protein</tok> <tok cat="CD">76</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SLP-76</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">interacts</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">guanine</tok> <tok cat="NN">nucleotide</tok> <tok cat="NN">exchange</tok> <tok cat="NN">factor</tok> <tok cat="NN">Vav</tok></cons></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="IN">of</tok> <tok cat="VBN">activated</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-AT</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="PRPP">its</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">development</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
1020904104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">cloning</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">characterization</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="JJ">Grb2-like</tok> <tok cat="NN">adaptor</tok> <tok cat="NN">molecule</tok></cons> <cons cat="VP"><tok cat="VBN">designated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Grb2-related</tok> <tok cat="NN">protein</tok> <tok cat="IN">of</tok> <tok cat="DT">the</tok> <tok cat="JJ">lymphoid</tok> <tok cat="NN">system</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GrpL</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1020904105@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GrpL</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="VBN">restricted</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NNS">tissues</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">distinguished</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">Grb2</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">having</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">proline-rich</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1020904106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">GrpL</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">coimmunoprecipitated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">SLP-76</tok></cons></cons> <tok cat="CC">but</tok> <tok cat="RB">not</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Sos1</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">Sos2</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lysates</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1020904107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><tok cat="NN">Grb2</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">coimmunoprecipitated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Sos1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Sos2</tok></cons></cons></cons> <tok cat="CC">but</tok> <tok cat="RB">not</tok> <cons cat="PP"><tok cat="IN">with</tok> <tok cat="NN">SLP-76</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1020904108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <tok cat="JJ">tyrosine-phosphorylated</tok> <tok cat="NN">LAT/pp36/38</tok> <tok cat="IN">in</tok> <tok cat="JJ">detergent</tok> <tok cat="NNS">lysates</tok> <tok cat="VBN">prepared</tok> <tok cat="IN">from</tok> <tok cat="NN">anti-CD3</tok> <tok cat="VBD">stimulated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok> <tok cat="VBN">associated</tok> <tok cat="IN">with</tok> <tok cat="NN">Grb2</tok> <tok cat="CC">but</tok> <tok cat="RB">not</tok> <tok cat="NN">GrpL</tok><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1020904109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">reveal</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">distinct</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="PP"><tok cat="VBG">involving</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">GrpL</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Grb2</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1020904110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">functional</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">GrpL-SLP-76</tok> <tok cat="NN">complex</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">suggested</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok></cons> <cons cat="S" ref="i8" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GrpL</tok></cons></cons> <cons cat="S" id="i8"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">act</tok> <cons cat="UCP" syn="COOD"><cons cat="ADVP"><tok cat="RB">alone</tok></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">concert</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">SLP-76</tok></cons></cons></cons></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">augment</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-AT</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
1021032101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">Unexpected</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="VBN">coordinated</tok></cons></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Spi-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Fli-1</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">megakaryocytic</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">four</tok> <tok cat="JJ">Epo-dependent</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="VP"><tok cat="VBN">established</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transgenic</tok> <tok cat="NNS">mice</tok></cons> <cons cat="VP"><tok cat="VBG">displaying</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">erythroid-specific</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">thermosensitive</tok> <tok cat="NN">SV40</tok> <tok cat="NN">T</tok> <tok cat="NN">antigen</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
1021032102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJS">Most</tok> <tok cat="JJ">erythroleukemic</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="VP"><tok cat="VBN">established</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">coexpress</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">erythrocytic</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">megakaryocytic</tok></cons></cons> <tok cat="NNS">markers</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" id="i2" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">often</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Spi-1</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons><tok cat="CC">and/or</tok> <cons cat="NP"><tok cat="NN">Fli-1</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons><cons cat="NP" id="i3"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="ADJP"><tok cat="VBN">known</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">transactivators</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">megakaryocyte-specific</tok> <tok cat="NNS">promoters</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
1021032103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">possibility</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">establishing</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">new</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="VP"><tok cat="VBG">keeping</tok> <cons cat="ADVP"><tok cat="RB">strictly</tok></cons> <cons cat="NP"><tok cat="JJ">erythroid-specific</tok> <tok cat="NNS">properties</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="VBN">targeted</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">conditional</tok></cons></cons> <tok cat="NN">immortalization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">erythrocytic</tok> <tok cat="NNS">progenitors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1021032104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">For</tok> <cons cat="NP"><tok cat="DT">that</tok> <tok cat="NN">purpose</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">established</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NNS">lines</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transgenic</tok> <tok cat="NNS">mice</tok></cons> <cons cat="VP"><tok cat="VBG">displaying</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">erythroid-specific</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">thermosensitive</tok> <tok cat="NN">SV40</tok> <tok cat="NN">T</tok> <tok cat="NN">antigen</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1021032105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">As</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">expected</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="JJ">transgenic</tok> <tok cat="NNS">mice</tok></cons> <cons cat="VP"><tok cat="VBD">developed</tok> <cons cat="NP"><tok cat="NN">splenomegaly</tok></cons> <cons cat="PP"><tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">massive</tok> <tok cat="NN">amplification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Ter</tok> <tok cat="CD">119</tok> <tok cat="JJ">positive</tok> <tok cat="JJ">erythroid</tok> <tok cat="JJ">nucleated</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">antigen</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1021032106@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="PP"><tok cat="IN">Despite</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">drastic</tok> <tok cat="NN">effect</tok></cons> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="FW">in</tok> <tok cat="FW">vitro</tok> <tok cat="NN">immortalization</tok> <tok cat="IN">of</tok> <tok cat="JJ">erythropoietin-dependent</tok> <tok cat="JJ">erythroid</tok> <tok cat="NNS">progenitors</tok></cons> <cons cat="ADVP"><tok cat="RB">unexpectedly</tok></cons> <cons cat="VP"><tok cat="VBD">occurred</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="JJ">low</tok> <tok cat="NN">frequency</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="CD">four</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="VP"><tok cat="VBN">established</tok> <cons cat="NP" null="NONE"></cons></cons></cons><cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="JJ">erythrocytic</tok> <tok cat="LRB">(</tok><tok cat="NNS">globins</tok><tok cat="RRB">)</tok> <cons cat="NP" ref="i4" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">megakaryocytic</tok> <cons cat="NP" ref="i4" null="RNR"></cons></cons><cons cat="NP" id="i4"><tok cat="NNS">markers</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">glycoprotein</tok> <tok cat="NN">IIb</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">platelet</tok> <tok cat="NN">factor</tok> <tok cat="CD">4</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Spi-1</tok> <cons cat="NP" ref="i5" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Fli-1</tok> <cons cat="NP" ref="i5" null="RNR"></cons></cons><cons cat="NP" id="i5"><tok cat="NNS">transcripts</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="JJ">permissive</tok> <tok cat="NN">temperature</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@55@@oe@16-12-2010
1021032107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" role="SBJ"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Switching</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nonpermissive</tok> <tok cat="NN">temperature</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">led</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">marked</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">globin</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">concomitant</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">expression</tok> <tok cat="IN">of</tok> <tok cat="NN">Spi-1</tok><tok cat="COMMA">,</tok> <tok cat="NN">Fli-1</tok><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <tok cat="JJ">megakaryocytic</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">erythropoietin-dependent</tok> <tok cat="NN">manner</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
1021032108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Interestingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="VBN">enhanced</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Spi-1</tok> <cons cat="NP" ref="i7" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Fli-1</tok> <cons cat="NP" ref="i7" null="RNR"></cons></cons><cons cat="NP" id="i7"><tok cat="NNS">genes</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">already</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Ter</tok> <tok cat="CD">119</tok> <tok cat="JJ">positive</tok> <tok cat="NN">cell</tok> <tok cat="NN">population</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transgenic</tok> <tok cat="NNS">mice</tok> <tok cat="NN">spleen</tok></cons> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1021032109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">like</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">Ter</tok> <tok cat="CD">119</tok> <tok cat="JJ">erythroid</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">these</tok> <tok cat="NN">Ter</tok> <tok cat="CD">119</tok> <tok cat="JJ">positive</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">transgenic</tok> <tok cat="NNS">mice</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">still</tok></cons> <cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">beta-globin</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP" syn="COOD"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="RB">very</tok> <tok cat="JJ">low</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">undetectable</tok></cons></cons> <cons cat="NP"><tok cat="NN">glycoprotein</tok> <tok cat="NN">IIb</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">platelet</tok> <tok cat="NN">factor</tok> <tok cat="CD">4</tok> <tok cat="JJ">megakaryocytic</tok> <tok cat="NNS">transcripts</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
1021032110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" id="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">Taken</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">together</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">unexpected</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">megakaryocytic</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">specific</tok> <tok cat="NN">property</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">immortalized</tok> <tok cat="NNS">cells</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">can</tok><tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">explained</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="VBN">enhanced</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Spi-1</tok> <cons cat="NP" ref="i10" null="RNR"></cons></cons><tok cat="CC">and/or</tok> <cons cat="NP"><tok cat="NN">Fli-1</tok> <cons cat="NP" ref="i10" null="RNR"></cons></cons><cons cat="NP" id="i10"><tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1021032201@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Retinoic</tok> <tok cat="NN">acid</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">CD34+</tok> <tok cat="JJ">hematopoietic</tok> <tok cat="NN">progenitor</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok> <tok cat="NNS">receptors</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">retinoid</tok> <tok cat="NN">X</tok> <tok cat="NNS">receptors</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">depends</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lineage</tok> <tok cat="NN">commitment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">hematopoietic</tok> <tok cat="NN">progenitor</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@30@@oe@16-12-2010
1021032202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">Retinoids</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">bifunctional</tok> <tok cat="NNS">regulators</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">growth</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1021032203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">explored</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">CD34+</tok> <tok cat="JJ">hematopoietic</tok> <tok cat="NN">progenitor</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">bone</tok> <tok cat="NN">marrow</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1021032204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NN">RA</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">100</tok> <tok cat="NN">nM</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">percentage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">dead</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="CD">24</tok><tok cat="NN">%</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="CD">44</tok><tok cat="NN">%</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="NN">day</tok> <tok cat="CD">6</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">p</tok></cons> <cons cat="NP" role="PRD"><tok cat="JJR">&lt;</tok> <tok cat="CD">0.05</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">n</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">6</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" ref="i1" id="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">control</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">cultured</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">medium</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
1021032205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">effect</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="NN">dose</tok> <tok cat="JJ">dependent</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">appeared</tok> <cons cat="ADJP" adv="TMP" role="PRD"><tok cat="RB">relatively</tok> <tok cat="JJ">late</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
1021032206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="JJ">Significant</tok> <tok cat="NNS">differences</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">day</tok> <tok cat="CD">4</tok></cons> <cons cat="ADVP"><tok cat="RB">onward</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
1021032207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i4" role="SBJ"><cons cat="NP"><tok cat="NN">Apoptosis</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="VBN">programmed</tok> <tok cat="NN">cell</tok> <tok cat="NN">death</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mode</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">death</tok></cons></cons></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">TUNEL</tok> <tok cat="NN">assay</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">detects</tok> <cons cat="NP"><tok cat="JJ">single</tok> <tok cat="NN">strand</tok> <tok cat="NNS">nicks</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">DNA</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Nicoletti</tok> <tok cat="NN">technique</tok></cons> <cons cat="VP"><tok cat="VBG">demonstrating</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">subdiploid</tok> <tok cat="NN">population</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">staining</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
1021032208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">RA</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">granulocyte</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok><tok cat="COLON">--</tok><cons cat="ADJP" null="QSTN"></cons></cons><cons cat="CONJP"><tok cat="CC">and</tok> <tok cat="RB">not</tok></cons> <cons cat="ADJP"><tok cat="JJ">granulocyte-macrophage</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok><tok cat="COLON">--</tok><tok cat="VBN">stimulated</tok></cons></cons> <tok cat="NN">proliferation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">CD34+</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1021032209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">RA</tok></cons> <cons cat="VP"><tok cat="VBD">opposed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">anti-apoptotic</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">G-CSF</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">GM-CSF</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">CD34+</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP" id="i7"><tok cat="NN">G-CSF</tok></cons><tok cat="COLON">:</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" id="i8"><tok cat="CD">8</tok><tok cat="NN">%</tok></cons> <cons cat="NP"><tok cat="JJ">dead</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="NN">day</tok> <tok cat="CD">6</tok></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><cons map="i7" cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">G-CSF</tok></cons> <tok cat="CC">+</tok> <cons cat="NP"><tok cat="NN">RA</tok></cons></cons><tok cat="COLON">:</tok> <cons map="i8" cat="NP"><tok cat="CD">20</tok><tok cat="NN">%</tok></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><cons map="i7" cat="NP"><tok cat="NN">GM-CSF</tok></cons><tok cat="COLON">:</tok> <cons map="i8" cat="NP"><tok cat="CD">12</tok><tok cat="NN">%</tok></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><cons map="i7" cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">GM-CSF</tok></cons> <tok cat="CC">+</tok> <cons cat="NP"><tok cat="NN">RA</tok></cons></cons><tok cat="COLON">:</tok> <cons map="i8" cat="NP"><tok cat="CD">27</tok><tok cat="NN">%</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@16-12-2010
1021032210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">RA</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">CD34+</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">CD34+CD71+</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">erythropoietin</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1021032211@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">explore</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">receptor</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">RA-induced</tok> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">used</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">selective</tok> <tok cat="NNS">ligands</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NNS">RARs</tok></cons><tok cat="COLON">;</tok> <cons cat="NP"><tok cat="NN">RO13-7410</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">retinoid</tok> <tok cat="NN">X</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NNS">RXRs</tok></cons><tok cat="COLON">;</tok> <cons cat="NP"><tok cat="NN">RO</tok> <tok cat="CD">25-6603</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1021032212@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="NNS">RARs</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">RA-mediated</tok> <tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NN">progenitor</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i11" role="SBJ"><cons cat="NP"><tok cat="NNS">RARs</tok></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><tok cat="NNS">RXRs</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">RA-mediated</tok> <tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NN">progenitor</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1021064501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">LPS-Induced</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">release</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" syn="COOD" role="PRD"><cons cat="ADJP"><tok cat="NN">protein</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok> <tok cat="JJ">dependent</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok> <tok cat="JJ">independent</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1021064502@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">BACKGROUND</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">mediator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">septic</tok> <tok cat="NN">shock</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1021064503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Endotoxin</tok> <tok cat="LRB">(</tok><tok cat="NN">LPS</tok><tok cat="RRB">)</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">release</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1021064504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Previous</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PKC</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">tyrosine</tok> <tok cat="NNS">kinases</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PTK</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">LPS-induced</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">production</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1021064505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">hypothesized</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="NN">PKC</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">PTK</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">would</tok> <cons cat="VP"><tok cat="VB">decrease</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">LPS-induced</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">release</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1021064506@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">MATERIALS</tok></cons> <tok cat="CC">AND</tok> <cons cat="NP"><tok cat="NNS">METHODS</tok></cons><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="JJ">Human</tok> <tok cat="NNS">monocytes</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">PMA</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">50</tok> <tok cat="NN">ng/ml</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">LPS</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">100</tok> <tok cat="NN">ng/ml</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="PP"><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><cons cat="NP" syn="COOD" id="i2"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">nonspecific</tok> <tok cat="NN">serine/threonine</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">inhibitor</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">staurosporine</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Stauro</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">specific</tok> <tok cat="NN">pan-PKC</tok> <tok cat="NN">inhibitor</tok> <tok cat="NN">bisindolylmaleimide</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Bis</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PTK</tok></cons></cons></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">genistein</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Gen</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@54@@oe@16-12-2010
1021064507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">release</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">culture</tok> <tok cat="NNS">supernatants</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">measured</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">an</tok> <tok cat="NN">ELISA</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
1021064508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">evaluated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
1021064509@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">RESULTS</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">LPS</tok></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">release</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1021064510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Nonspecific</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">release</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">PKC</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">Bis</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">LPS-induced</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">release</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1021064511@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">PTK</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">Gen</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">attenuated</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="JJ">LPS-induced</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">production</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1021064512@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Direct</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PKC</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">PMA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">production</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1021064513@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">CONCLUSIONS</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">LPS-induced</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">release</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">independent</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PKC</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1021064514@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">evidence</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PTK</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">LPS-induced</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">release</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potential</tok> <tok cat="JJ">therapeutic</tok> <tok cat="NN">target</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NNS">states</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1021064515@GENIA Treebank@formal@@1@S@<cons cat="NP"><tok cat="NN">Copyright</tok> <tok cat="CD">1999</tok> <tok cat="NNP">Academic</tok> <tok cat="NNP">Press</tok><tok cat="PERIOD">.</tok></cons>@@@@1@5@@oe@16-12-2010
1021238901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">Dicarba-closo-dodecaboranes</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">pharmacophore</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@5@@oe@16-12-2010
1021238902@GENIA Treebank@formal@@1@S@<cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">Retinoidal</tok> <tok cat="NNS">antagonists</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">potential</tok> <tok cat="NNS">agonists</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@6@@oe@16-12-2010
1021238903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">Synthesis</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">biological</tok></cons></cons> <tok cat="NN">evaluation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">dicarba-closo-dodecaborane</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">carborane</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">derivatives</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">retinoids</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">described</tok><cons cat="NP" ref="i1" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1021238904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Their</tok> <tok cat="JJ">retinoidal</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">terms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">differentiation-inducing</tok> <tok cat="NN">ability</tok></cons> <cons cat="PP"><tok cat="IN">toward</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">promyelocytic</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">HL-60</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1021238905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">High</tok> <tok cat="JJ">retinoidal</tok> <tok cat="NN">activity</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">agonist</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">antagonist</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RAR</tok></cons></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">carboxylic</tok> <tok cat="NN">acid</tok> <tok cat="NN">moiety</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">appropriate</tok> <tok cat="JJ">hydrophobic</tok> <tok cat="NN">group</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">suitable</tok> <tok cat="NN">position</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">molecule</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1021238906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">4-carboranyl-substituted</tok> <tok cat="NNS">compounds</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="CD">7</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">11</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">antagonistic</tok> <tok cat="NN">activity</tok></cons> <tok cat="CC">but</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="JJ">agonistic</tok> <tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">even</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">potent</tok> <tok cat="NN">synergist</tok> <tok cat="NN">HX630</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1021238907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">On</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">other</tok> <tok cat="NN">hand</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">3-carboranyl-substituted</tok> <tok cat="NNS">compounds</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="CD">8</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">12</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">potential</tok> <tok cat="JJ">agonistic</tok> <tok cat="NN">activity</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="JJ">antagonistic</tok> <tok cat="NN">activity</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1021238908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBZ">indicates</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">carboranes</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">applicable</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">hydrophobic</tok> <tok cat="NN">moiety</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="RB">biologically</tok> <tok cat="JJ">active</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1021485401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Modulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">growth</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">Ras</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1021485402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">As</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">result</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="VBG">transforming</tok> <tok cat="NNS">abilities</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i1" role="SBJ"><tok cat="VBN">activated</tok> <tok cat="NN">Ras</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">great</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">cancers</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1021485403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">ras</tok> <tok cat="NN">mutation</tok> <tok cat="NN">frequency</tok></cons> <cons cat="VP"><tok cat="VBZ">varies</tok> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="CD">95</tok><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">pancreatic</tok> <tok cat="NN">cancer</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">5</tok><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">breast</tok> <tok cat="NN">cancer</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1021485404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">leukemia</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJS">highest</tok> <tok cat="NN">frequency</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">30</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">leukemia</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1021485405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ras</tok> <tok cat="NNS">mutations</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">correlated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">poor</tok> <tok cat="NN">prognosis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">negative</tok> <tok cat="JJ">clinical</tok> <tok cat="NN">outcome</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1021485406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="VBN">mutated</tok> <tok cat="NN">Ras</tok></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">mechanisms</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">favor</tok> <cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">growth</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBP">enhance</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">metastatic</tok> <tok cat="NN">capacity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">tumors</tok></cons></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="VP"><tok cat="VBP">modulate</tok> <cons cat="NP"><tok cat="JJ">tumor-specific</tok> <tok cat="JJ">immune</tok> <tok cat="NNS">responses</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1021485407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Several</tok> <tok cat="JJ">new</tok> <tok cat="NNS">functions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Ras</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">downregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">major</tok> <tok cat="NN">histocompatibility</tok> <tok cat="NN">complex</tok> <tok cat="NNS">molecules</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">upregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="JJ">certain</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">growth</tok> <tok cat="NNS">factors</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">degradative</tok> <tok cat="NNS">enzymes</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="JJ">uncovered</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">last</tok> <tok cat="NN">decade</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1021485408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Additionally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="VBN">mutated</tok> <tok cat="NN">Ras</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VB">serve</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">primary</tok> <tok cat="NN">target</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">development</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">immunotherapy</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">drug</tok> <tok cat="NN">therapy</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1021485409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">review</tok></cons> <cons cat="VP"><tok cat="MD">will</tok> <cons cat="VP"><tok cat="VB">discuss</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i6"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="NN">Ras</tok></cons> <cons cat="VP"><tok cat="NN">expressing</tok> <cons cat="NP"><tok cat="NNS">tumors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">evade</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">destruction</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immune</tok> <tok cat="NN">system</tok></cons></cons></cons> <cons cat="PP" ref="i6" null="T"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">enhance</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">growth</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">metastatic</tok> <tok cat="NN">potential</tok></cons></cons><cons cat="PP" ref="i6" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1021485410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="MD">will</tok> <cons cat="ADVP"><tok cat="RB">further</tok></cons> <cons cat="VP"><tok cat="VB">elaborate</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">attempts</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">develop</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">successful</tok> <tok cat="NN">immunotherapy</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">drug</tok> <tok cat="NN">therapy</tok></cons> <cons cat="VP"><tok cat="VBG">targeting</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Ras</tok></cons> <cons cat="VP"><tok cat="NN">expressing</tok> <cons cat="NP"><tok cat="NNS">tumors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1021607101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">E2A-HLF</tok> <tok cat="JJ">chimeric</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">apoptosis</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">maturation</tok> <tok cat="NN">arrest</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">development</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">lymphoblastic</tok> <tok cat="NN">leukemia</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1021607102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">E2A-HLF</tok> <tok cat="NN">fusion</tok> <tok cat="NN">gene</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">generated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">t(17;19)(q22;p13)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">lymphoblastic</tok> <tok cat="NN">leukemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ALL</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">encodes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">chimeric</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">trans-activating</tok> <tok cat="NNS">domains</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">E2A</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">fused</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">DNA- binding</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">dimerization</tok></cons></cons> <tok cat="NNS">domains</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">hepatic</tok> <tok cat="JJ">leukemic</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HLF</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@16-12-2010
1021607103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">investigate</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">biological</tok> <tok cat="NN">role</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i3" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">generated</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transgenic</tok> <tok cat="NNS">mice</tok></cons> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP"><tok cat="NN">E2A-HLF</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Ig</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">enhancer</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">promoter</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">direct</tok> <cons cat="NP"><tok cat="NN">transgene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="ADJP"><tok cat="VBN">committed</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">lymphoid</tok> <tok cat="NN">lineage</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1021607104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">transgenic</tok> <tok cat="NNS">mice</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">exhibited</tok> <cons cat="NP"><tok cat="JJ">abnormal</tok> <tok cat="NN">development</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">thymus</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">spleen</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">susceptible</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">infection</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1021607105@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">thymus</tok></cons> <cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">small</tok> <tok cat="NNS">numbers</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">thymocytes</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">TUNEL</tok> <tok cat="NN">staining</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJR">higher</tok> <tok cat="NN">population</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">thymocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBG">undergoing</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1021607106@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">spleen</tok></cons> <cons cat="VP"><tok cat="VBD">exhibited</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">marked</tok> <tok cat="NN">reduction</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">splenic</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">flow</tok> <tok cat="JJ">cytometric</tok> <tok cat="NNS">analyses</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="FW">in</tok> <tok cat="FW">vitro</tok> <tok cat="NN">colony</tok> <tok cat="NN">formation</tok> <tok cat="NNS">assays</tok></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">B-cell</tok> <tok cat="NN">maturation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="RB">very</tok> <tok cat="JJ">early</tok> <tok cat="JJ">developmental</tok> <tok cat="NN">stage</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@16-12-2010
1021607107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBD">indicated</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">E2A-HLF</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">apoptosis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">maturation</tok> <tok cat="NN">arrest</tok></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">susceptibility</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transgenic</tok> <tok cat="NNS">mice</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">infection</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="PP" role="PRD"><tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">immunodeficiency</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1021607108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="JJ">several</tok> <tok cat="JJ">transgenic</tok> <tok cat="NNS">mice</tok></cons> <cons cat="VP"><tok cat="VBD">developed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="NN">leukemia</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">classified</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">T-ALL</tok></cons></cons> <cons cat="PP"><tok cat="VBN">based</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">surface</tok> <tok cat="NN">marker</tok> <tok cat="NN">analysis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NNS">rearrangements</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">an</tok> <tok cat="JJ">additional</tok> <tok cat="NN">event</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">malignant</tok> <tok cat="NN">transformation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">lymphoid</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP"><tok cat="NN">E2A-HLF</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
1021607109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">insight</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">biological</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">E2A-HLF</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">lymphoid</tok> <tok cat="NN">development</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">leukemogenesis</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1021753401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Cellular</tok> <tok cat="NN">disposition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">sulphamethoxazole</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NNS">metabolites</tok></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><tok cat="NNS">implications</tok> <tok cat="IN">for</tok> <tok cat="NN">hypersensitivity</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1021753402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="LST"><tok cat="LS">1.</tok></cons> <cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NN">Bioactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">sulphamethoxazole</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SMX</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">chemically-reactive</tok> <tok cat="NNS">metabolites</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">subsequent</tok> <tok cat="NN">protein</tok> <tok cat="NN">conjugation</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">thought</tok> <cons cat="S"><cons cat="NP" ref="i1" id="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">SMX</tok> <tok cat="NN">hypersensitivity</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1021753403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NN">metabolism</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">disposition</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">conjugation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SMX</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NNS">metabolites</tok></cons> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1021753404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="LST"><tok cat="FW">2.</tok></cons> <cons cat="NP" role="SBJ"><tok cat="NN">Flow</tok> <tok cat="NN">cytometry</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">N-hydroxy</tok> <tok cat="LRB">(</tok><tok cat="NN">SMX-NHOH</tok><tok cat="RRB">)</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">nitroso</tok> <tok cat="LRB">(</tok><tok cat="NN">SMX-NO</tok><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">metabolites</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SMX</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <tok cat="RB">not</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">SMX</tok></cons> <cons cat="NP"><tok cat="PRP">itself</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">surface</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <tok cat="JJ">viable</tok> <cons cat="NP"><tok cat="JJ">white</tok> <tok cat="NN">blood</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
1021753405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="JJ">Cellular</tok> <tok cat="NN">haptenation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">SMX-NO</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">reduced</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">exogenous</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glutathione</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GSH</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
1021753406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="LST"><tok cat="LS">3.</tok></cons> <cons cat="NP" syn="COOD" id="i4" role="SBJ"><cons cat="NP"><tok cat="NN">SMX-NHOH</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">SMX-NO</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">rapidly</tok></cons> <cons cat="VP"><tok cat="VBN">reduced</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><cons cat="ADVP"><tok cat="RB">back</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">parent</tok> <tok cat="NN">compound</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">cysteine</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CYS</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">GSH</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">plasma</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">an</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">important</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">ubiquitous</tok></cons></cons> <tok cat="NN">bioinactivation</tok> <tok cat="NN">mechanism</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
1021753407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="LST"><tok cat="LS">4.</tok></cons> <cons cat="NP" role="SBJ"><tok cat="NN">Fluorescence</tok> <tok cat="NN">HPLC</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">SMX-NHOH</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">SMX-NO</tok></cons></cons> <cons cat="VP"><tok cat="VBD">depleted</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CYS</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">GSH</tok></cons></cons> <cons cat="PP" id="i5"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">buffer</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJR">lesser</tok> <tok cat="NN">extent</tok></cons></cons><tok cat="COMMA">,</tok> <cons map="i5" cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">plasma</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1021753408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="LST"><tok cat="LS">5.</tok></cons> <cons cat="NP" syn="COOD" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">Neutrophil</tok> <tok cat="NN">apoptosis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">neutrophil</tok> <tok cat="NN">function</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJR">lower</tok> <tok cat="NNS">concentrations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">SMX-NHOH</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">SMX-NO</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="VP"><tok cat="VBG">inducing</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">loss</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">membrane</tok> <tok cat="NN">viability</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">SMX</tok></cons> <cons cat="VP"><tok cat="VBG">having</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">effect</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
1021753409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">Lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons><cons cat="VP"><tok cat="JJR">&lt;</tok><cons cat="NP"><tok cat="CD">0.05</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="RBR">more</tok> <tok cat="JJ">sensitive</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">direct</tok> <tok cat="JJ">cytotoxic</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SMX-NO</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><tok cat="NNS">neutrophils</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1021753410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="LST"><tok cat="LS">6.</tok></cons> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Partitioning</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SMX-NHOH</tok></cons></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="JJ">red</tok> <tok cat="NN">blood</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons><cons cat="VP"><tok cat="JJR">&lt;</tok><cons cat="NP"><tok cat="CD">0.05</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="ADJP" role="PRD"><tok cat="JJR">lower</tok> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">hydroxylamine</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">dapsone</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1021753411@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="LST"><tok cat="LS">7.</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">balance</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">oxidation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SMX</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">toxic</tok> <tok cat="NNS">metabolites</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">reduction</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="JJ">protective</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">mechanism</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1021753412@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">If</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">an</tok> <tok cat="NN">imbalance</tok></cons> <cons cat="VP"><tok cat="VBZ">exists</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">haptenation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">toxic</tok> <tok cat="NNS">metabolites</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">bodily</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">surface</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">viable</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">occur</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">result</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">drug</tok> <tok cat="NN">hypersensitivity</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1022125001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Leukocyte</tok> <tok cat="NNS">populations</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">hormone</tok> <tok cat="NNS">receptors</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">eutopic</tok> <cons cat="NP" ref="i6" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">ectopic</tok> <cons cat="NP" ref="i6" null="RNR"></cons></cons><cons cat="NP" id="i6"><tok cat="JJ">first</tok> <tok cat="NN">trimester</tok> <tok cat="JJ">human</tok> <tok cat="NNS">pregnancies</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1022125002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">implantation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">trophoblast</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="JJ">extrauterine</tok> <tok cat="NNS">sites</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">still</tok></cons> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">decidualization</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
1022125003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">objective</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="S" role="PRD"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">compare</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">decidualization</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">eutopic</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">ectopic</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><cons cat="NP" id="i1"><tok cat="NN">implantation</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1022125004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="NNS">Tissues</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">women</tok></cons> <cons cat="VP"><tok cat="VBG">undergoing</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">elective</tok> <tok cat="NN">termination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">uterine</tok> <tok cat="NN">pregnancy</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">women</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">ectopic</tok> <tok cat="NN">pregnancy</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">detect</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="ADJP"><tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">maintenance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">pregnancy</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">BCL-2+</tok><cons cat="NP" ref="i3" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">CD56+</tok><cons cat="NP" ref="i3" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">CD3+</tok><cons cat="NP" ref="i3" null="RNR"></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">CD8+</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">CD68+</tok> <cons cat="NP" ref="i3" null="RNR"></cons></cons><cons cat="NP" id="i3"><tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">oestrogen</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ER</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">progesterone</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">immunohistochemistry</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@60@@oe@16-12-2010
1022125005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="FW">In-situ</tok> <tok cat="NN">detection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">fragmented</tok> <tok cat="NN">DNA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">performed</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">identify</tok> <cons cat="NP"><tok cat="JJ">apoptotic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1022125006@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">percentage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">CD3+</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">among</tok> <cons cat="NP"><tok cat="DT">all</tok> <tok cat="JJ">immunocompetent</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">tubal</tok> <tok cat="NN">epithelium</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><tok cat="CD">46.6</tok><tok cat="NN">%</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">39.9</tok><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">CD3+</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="NP" role="PRD"><tok cat="JJ">CD8+</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">other</tok> <tok cat="CD">53.4</tok><tok cat="NN">%</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="NP" role="PRD"><tok cat="JJ">CD68+</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
1022125007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">CD56+</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">undetectable</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">ectopic</tok> <tok cat="NN">decidua</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">feto-maternal</tok> <tok cat="NN">interface</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">ectopic</tok> <tok cat="NN">tissue</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1022125008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">uterine</tok> <tok cat="NN">decidua</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="CD">29.9</tok><tok cat="NN">%</tok> <tok cat="JJ">CD3+</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">2.2</tok><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">CD3+</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="NP" role="PRD"><tok cat="JJ">CD8+</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">51.6</tok><tok cat="NN">%</tok> <tok cat="JJ">CD56+</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">18.5</tok><tok cat="NN">%</tok> <tok cat="JJ">CD68+</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1022125009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">ratio</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">BCL2+</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">CD3+</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">ectopic</tok> <tok cat="NN">pregnancy</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><tok cat="CD">0.41</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
1022125010@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">uterine</tok> <tok cat="NN">pregnancy</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ratio</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">BCL-2</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">CD3</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" syn="COOD" role="PRD"><cons cat="NP"><tok cat="CD">0.44</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">0.39</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">CD56</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1022125011@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Tissues</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="JJ">ectopic</tok> <cons cat="NP" ref="i5" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">uterine</tok> <cons cat="NP" ref="i5" null="RNR"></cons></cons><cons cat="NP" id="i5"><tok cat="NNS">pregnancies</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">positive</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">PR</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1022125012@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJR">Fewer</tok> <tok cat="JJ">apoptotic</tok> <tok cat="NN">cell</tok> <tok cat="NNS">bodies</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">ectopic</tok> <tok cat="NN">pregnancy</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
1022125013@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">use</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tissue</tok></cons> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">ectopic</tok> <tok cat="NN">pregnancy</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">become</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">excellent</tok> <tok cat="NN">model</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">identify</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">trophoblast</tok> <tok cat="NN">invasion</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">eutopic</tok> <tok cat="NNS">pregnancies</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1022164301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Tcf-1-mediated</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">differential</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">glycogen</tok> <tok cat="NN">synthase</tok> <tok cat="NN">kinase-3</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">fibroblasts</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1022164302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Beta-catenin</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">vertebrate</tok> <tok cat="NN">homolog</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Drosophila</tok> <tok cat="NN">segment</tok> <tok cat="NN">polarity</tok> <tok cat="NN">gene</tok> <tok cat="NN">Armadillo</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="NNS">roles</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="JJ">cell-cell</tok> <tok cat="NN">adhesion</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">transduction</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Wnt</tok> <tok cat="NN">signaling</tok> <tok cat="NN">cascade</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@16-12-2010
1022164303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Recently</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Lef/Tcf</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">family</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NNS">partners</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">beta-catenin</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">explaining</tok> <cons cat="SBAR"><cons cat="WHADVP" id="i2"><tok cat="WRB">how</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">beta-catenin</tok></cons> <cons cat="VP"><tok cat="VBZ">alters</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons><cons cat="ADVP" ref="i2" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1022164304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">Tcf-1</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">becomes</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">transcriptionally</tok> <tok cat="JJ">active</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">beta-catenin</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">Wnt</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok> <tok cat="NN">pathway</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">operational</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="PP"><tok cat="RB">as</tok> <cons cat="ADVP"><tok cat="RB">well</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@16-12-2010
1022164305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="NN">Wnt</tok> <tok cat="NNS">signals</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">negative</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">glycogen</tok> <tok cat="NN">synthase</tok> <tok cat="NN">kinase-3beta</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GSK-3beta</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">beta-catenin</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">find</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GSK-3beta</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Tcf-mediated</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@16-12-2010
1022164306@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="ADVP"><tok cat="DT">That</tok> <tok cat="VBZ">is</tok></cons><tok cat="COMMA">,</tok> <cons cat="S"><tok cat="DT">a</tok> <tok cat="JJ">dominant</tok> <tok cat="JJ">negative</tok> <tok cat="NN">GSK-3beta</tok> <tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <tok cat="RB">specifically</tok> <tok cat="VB">activate</tok> <tok cat="NN">Tcf</tok> <tok cat="NN">transcription</tok></cons> <tok cat="CC">and</tok> <cons cat="S"><tok cat="NNS">stimuli</tok> <tok cat="LRB">(</tok><tok cat="NN">lithium</tok> <tok cat="CC">or</tok> <tok cat="NN">phytohemagglutinin</tok><tok cat="RRB">)</tok> <tok cat="WDT">that</tok> <tok cat="VBP">inhibit</tok> <tok cat="NN">GSK-3beta</tok> <tok cat="NN">activity</tok> <tok cat="RB">also</tok> <tok cat="VBP">do</tok> <tok cat="RB">not</tok> <tok cat="VB">activate</tok> <tok cat="NN">Tcf</tok> <tok cat="NN">reporter</tok> <tok cat="NNS">genes</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1022164307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GSK-3beta</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">insufficient</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Tcf-dependent</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1022164308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">C57MG</tok> <tok cat="NN">fibroblast</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">lithium</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">inactivates</tok> <cons cat="NP"><tok cat="NN">GSK-3beta</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><tok cat="JJ">Tcf-controlled</tok> <tok cat="NN">transcription</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1022164309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NN">demonstration</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">lithium</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">alter</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">Tcf-responsive</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">points</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">difference</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Wnt</tok> <tok cat="NN">signaling</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">fibroblasts</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1022165801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">CIITA-induced</tok> <tok cat="NN">occupation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NNS">promoters</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">independent</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cooperative</tok> <tok cat="NN">stabilization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">promoter-bound</tok> <tok cat="JJ">multi-protein</tok> <tok cat="NNS">complexes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1022165802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Precise</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">crucial</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1022165803@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">transactivator</tok> <tok cat="NN">CIITA</tok></cons> <cons cat="VP"><tok cat="VBZ">behaves</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">master</tok> <tok cat="NN">controller</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">constitutive</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">inducible</tok></cons></cons> <tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NN">gene</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">exact</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">action</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="VBN">known</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1022165804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NNS">promoters</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok> <tok cat="CD">three</tok></cons> <tok cat="JJ">distinct</tok> <tok cat="JJ">multi-protein</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">RFX</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">X2BP</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-Y</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1022165805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">stability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">binding</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cooperative</tok> <tok cat="NNS">interactions</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1022165806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CIITA</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="NNS">II- cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">triggers</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">occupation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">promoters</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">complexes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1022165807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">observation</tok></cons> <cons cat="VP"><tok cat="VBD">raised</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">possibility</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CIITA</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">occupation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cooperative</tok> <tok cat="NN">stabilization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">DNA-bound</tok> <tok cat="JJ">multi-protein</tok> <tok cat="NNS">complexes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1022165808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CIITA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">stability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">higher-order</tok> <tok cat="NN">protein</tok> <tok cat="NN">complex</tok></cons> <cons cat="VP"><tok cat="VBN">formed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">DNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">RFX</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">X2BP</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-Y</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1022165809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">CIITA-induced</tok> <tok cat="NN">promoter</tok> <tok cat="NN">occupancy</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">chromatin</tok> <tok cat="NN">structure</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">accessibility</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NNS">promoters</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1022165810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">This</tok> <tok cat="NN">ability</tok> <cons cat="S" ref="i2" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CIITA</tok></cons></cons> <cons cat="S" id="i2"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">facilitate</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">occupation</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">undissociable</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">transactivation</tok> <tok cat="NN">potential</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1022165811@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CIITA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">cell-type</tok> <tok cat="JJ">specific</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">since</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CIITA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">occupation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MHC</tok> <tok cat="NN">class</tok> <tok cat="CD">II</tok> <tok cat="NNS">promoters</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1022410901@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Immunosuppressant</tok></cons> <cons cat="NP"><tok cat="NN">PG490</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">triptolide</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">interleukin-2</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">purine-box/nuclear</tok> <tok cat="NN">factor</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NNS">T-cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1022410902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">PG490</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">triptolide</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">diterpene</tok> <tok cat="NN">triepoxide</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">potent</tok> <tok cat="JJ">immunosuppressive</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">antiinflammatory</tok> <cons cat="NP" ref="i1" null="RNR"></cons></cons><cons cat="NP" id="i1"><tok cat="NNS">properties</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1022410903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PG490</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin</tok></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="CD">-2</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">12-myristate</tok> <tok cat="NN">13-acetate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PMA</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">antibody</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">CD3</tok></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">IC50</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">10</tok> <tok cat="NN">ng/ml</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PMA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">ionomycin</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">Iono</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IC50</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">40</tok> <tok cat="NN">ng/ml</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@16-12-2010
1022410904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">T-cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">PG490</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><tok cat="JJ">PMA/Iono-stimulated</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">transcription</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@16-12-2010
1022410905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PG490</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">purine-box</tok></cons><tok cat="COLON">/</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">antigen</tok> <tok cat="NN">receptor</tok> <tok cat="NN">response</tok> <tok cat="NN">element</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ARRE</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COLON">/</tok><cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NNS">T-cells</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-AT</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">target</tok> <tok cat="NN">sequence</tok></cons></cons></cons> <tok cat="CC">but</tok> <tok cat="RB">not</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappaB</tok> <tok cat="NN">site</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@16-12-2010
1022410906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PG490</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">completely</tok></cons> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP" ref="i6" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">purine-box/ARRE/NF-AT</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <cons cat="NP" ref="i2" null="RNR"></cons></cons><cons cat="NP" id="i2"><tok cat="NN">target</tok> <tok cat="NN">DNA</tok> <tok cat="NNS">sequences</tok></cons></cons> <cons cat="VP" id="i6"><tok cat="VBN">triggered</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="NNS">stimuli</tok></cons> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" null="NONE"></cons></cons></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PMA</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">PMA/Iono</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor-alpha</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1022410907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PG490</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">PMA-stimulated</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">chimeric</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i3"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">C-terminal</tok> <tok cat="NN">TA1</tok> <tok cat="NN">transactivation</tok> <tok cat="NN">domain</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok> <tok cat="NN">p65</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">fused</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">domain</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GAL4</tok></cons></cons></cons></cons><cons cat="PP" ref="i3" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1022410908@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">16HBE</tok> <tok cat="JJ">human</tok> <tok cat="JJ">bronchial</tok> <tok cat="JJ">epithelial</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">IL-8</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="ADVP"><tok cat="RB">predominantly</tok></cons> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">PG490</tok></cons> <tok cat="CC">but</tok> <tok cat="RB">not</tok> <cons cat="NP"><tok cat="NN">cyclosporin</tok> <tok cat="NN">A</tok></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">completely</tok></cons> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-8</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@16-12-2010
1022410909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">PG490</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">differs</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">cyclosporin</tok> <tok cat="NN">A</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappaB</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">purine-box</tok> <tok cat="NN">regulator</tok> <tok cat="VBG">operating</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ARRE/NF-AT</tok> <tok cat="NN">site</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">step</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@16-12-2010
1022413501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Differential</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Smad6</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Smad7</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">bone</tok> <tok cat="JJ">morphogenetic</tok> <tok cat="NN">protein-</tok> <cons cat="NP" ref="i1" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP" id="i1"><cons cat="NP" null="QSTN"></cons><tok cat="JJ">activin-mediated</tok> <tok cat="NN">growth</tok> <tok cat="NN">arrest</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1022413502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Smad6</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Smad7</tok></cons></cons> <cons cat="VP"><tok cat="VBP">prevent</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">ligand-induced</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">signal-transducing</tok> <tok cat="NN">Smad</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBG">transforming</tok> <tok cat="NN">growth</tok> <tok cat="NN">factor-beta</tok> <tok cat="NN">family</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1022413503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">Smad6</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Smad7</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">bone</tok> <tok cat="JJ">morphogenetic</tok> <tok cat="NN">protein-2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">hBMP-2</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="JJ">-inducible</tok> <tok cat="NNS">antagonists</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">hBMP-2-induced</tok> <tok cat="NN">growth</tok> <tok cat="NN">arrest</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">mouse</tok> <tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">hybridoma</tok></cons> <cons cat="NP"><tok cat="NN">HS-72</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1022413504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">confirmed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">ectopic</tok> <tok cat="NNS">expressions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Smad6</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Smad7</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">hBMP-2-induced</tok> <tok cat="NN">Smad1/Smad5</tok> <tok cat="NN">phosphorylation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1022413505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP" role="PRD"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBD">reported</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Smad7</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">activin</tok> <tok cat="JJ">A-inducible</tok> <tok cat="NN">antagonist</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">activin</tok> <tok cat="JJ">A-induced</tok> <tok cat="NN">growth</tok> <tok cat="NN">arrest</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons> <cons cat="PP" id="i3"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">HS-72</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1022413506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Interestingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Smad6</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">activin</tok> <tok cat="NN">A</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">HS-72</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">Smad6</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="JJ">antagonistic</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">activin</tok> <tok cat="JJ">A-induced</tok> <tok cat="NN">growth</tok> <tok cat="NN">arrest</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1022413507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">ectopic</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Smad7</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <tok cat="RB">not</tok> <cons cat="NP"><tok cat="NN">Smad6</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activin</tok> <tok cat="JJ">A-induced</tok> <tok cat="NN">Smad2</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">HS-72</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1022413508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Smad6</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Smad7</tok></cons></cons> <cons cat="VP"><tok cat="VBP">exhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">differential</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">bone</tok> <tok cat="JJ">morphogenetic</tok> <tok cat="NN">protein-2-</tok> <cons cat="NP" ref="i5" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP" id="i5"><tok cat="NN">activin</tok> <tok cat="JJ">A-mediated</tok> <tok cat="NN">signaling</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">lineage</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1022415601@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Sp1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">AP-2</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">mediate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">acid</tok> <tok cat="NN">sphingomyelinase</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NN">differentiation</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@16-12-2010
1022415602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NNS">Cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">monocytic</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="NP"><tok cat="NN">THP-1</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">differentiate</tok> <cons cat="PP"><tok cat="IN">towards</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">macrophage-like</tok> <tok cat="NN">phenotype</tok></cons></cons> <cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i3"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">12-myristate -13- acetate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PMA</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">1,25-dihydroxy-vitamin</tok> <tok cat="NN">D3</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">various</tok> <tok cat="JJ">other</tok> <tok cat="NNS">agents</tok></cons></cons></cons><cons cat="ADVP" ref="i3" null="T"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
1022415603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">lysosomal</tok> <tok cat="NN">enzyme</tok></cons> <cons cat="NP"><tok cat="NN">acid</tok> <tok cat="NN">sphingomyelinase</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">ASM</tok></cons><tok cat="COLON">;</tok> <cons cat="NP"><tok cat="NN">E.C.3.1.4.12</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">process</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="JJ">elevated</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">differentiated</tok> <tok cat="NN">THP-1</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">differentiated</tok> <tok cat="JJ">human</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">phagocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@16-12-2010
1022415604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Northern</tok> <tok cat="NN">blotting</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">RNase</tok> <tok cat="NN">protection</tok> <tok cat="NN">assay</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="JJ">run-on</tok> <tok cat="NNS">analyses</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">up-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ASM</tok> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">mainly</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="JJ">new</tok> <tok cat="NN">protein</tok> <tok cat="NN">synthesis</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="VBN">enhanced</tok> <tok cat="NN">ASM</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
1022415605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">This</tok> <tok cat="JJ">cell-type</tok> <tok cat="JJ">specific</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">PMA</tok> <tok cat="NN">treatment</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">further</tok></cons> <cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">respect</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">control</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1022415606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">series</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">5'</tok> <tok cat="NN">deletion</tok> <tok cat="NNS">derivatives</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">upstream</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">transfection</tok> <tok cat="NNS">assays</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">identify</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NNS">elements</tok></cons> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">basal</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">inducible</tok></cons></cons> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@16-12-2010
1022415607@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">A</tok> <tok cat="NN">PMA</tok> <tok cat="JJ">responsive</tok> <tok cat="NN">element</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="JJ">localized</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">-319</tok> <tok cat="CC">and</tok> <tok cat="CD">-219</tok> <tok cat="NN">bp</tok> <tok cat="RB">upstream</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">initiation</tok> <tok cat="NN">codon</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">co-transfections</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">expression</tok> <tok cat="NNS">plasmids</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">AP-2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Sp1</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">augmented</tok> <tok cat="NN">ASM</tok> <tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" id="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">abolished</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i11"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i16" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBG">binding</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="CD">two</tok> <tok cat="NNS">factors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">deleted</tok><cons cat="NP" ref="i16" null="NONE"></cons><cons cat="ADVP" ref="i11" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@16-12-2010
1022415608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">supershift</tok> <tok cat="NNS">assays</tok></cons></cons></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">region</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="VP"><tok cat="VBN">bound</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">Sp1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">AP-2</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1022415609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">factors</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="VP"><tok cat="VBN">prepared</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADJP"><tok cat="VBN">induced</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">uninduced</tok></cons></cons> <tok cat="NN">THP-1</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1022415610@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i13" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">intensity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">complex</tok> <tok cat="VBN">formed</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">appeared</tok> <cons cat="S"><cons cat="NP" ref="i13" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">increase</tok> <cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i14"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i15" role="SBJ"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">PMA-treated</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">used</tok><cons cat="NP" ref="i15" null="NONE"></cons><cons cat="ADVP" ref="i14" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1022415611@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">From</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">studies</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">concerted</tok> <tok cat="NN">action</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">AP-2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Sp1</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">up -regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ASM</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">process</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">macrophage</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@16-12-2010
1022422301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Estrogen</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">progesterone</tok></cons></cons> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="PP"><cons cat="NP"><tok cat="NN">survival</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">monoblastoid</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">undergoing</tok> <cons cat="NP"><tok cat="JJ">TNF-alpha-induced</tok> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
1022422302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">mononucleated</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">physiological</tok> <tok cat="NN">process</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">intensity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1022422303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" id="i1" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">female</tok> <tok cat="NN">steroid</tok> <tok cat="NNS">hormones</tok></cons> <cons cat="NP"><tok cat="NN">estrogen</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">E2</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">progesterone</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Prog</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">modulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">reactivity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immune</tok> <tok cat="NN">system</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="ADVP"><tok cat="RB">recently</tok></cons> <cons cat="NP" id="i2" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">osteoblasts</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@16-12-2010
1022422304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="JJ">TNF-alpha-mediated</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="ADVP"><tok cat="RB">well</tok></cons> <cons cat="VP"><tok cat="VBN">characterized</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1022422305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">E2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Prog</tok></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">interfere</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">TNF-alpha-induced</tok> <tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">monoblastoid</tok> <tok cat="NN">U937</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1022422306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">E2</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">Prog</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><tok cat="NN">survival</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">prevented</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">TNF-alpha</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="CC">both</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">undifferentiated</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">macrophage-like</tok></cons></cons> <tok cat="JJ">PMA-differentiated</tok> <tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">assessed</tok> <cons cat="NP" null="NONE"></cons><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">trypan</tok> <tok cat="JJ">blue</tok> <tok cat="NN">exclusion</tok> <tok cat="NN">cell</tok> <tok cat="NN">counting</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">thymidine</tok> <tok cat="NN">incorporation</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">AnnexinV</tok> <tok cat="NN">labeling</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">followed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">flow</tok> <tok cat="NN">cytometry</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">fragmentation</tok> <tok cat="NNS">studies</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@16-12-2010
1022422307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">effect</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">hormone</tok> <tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">since</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">estrogen</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ER-alpha</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">ER-beta</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">progesterone</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PR</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">mRNAs</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">ER-alpha</tok> <tok cat="NN">protein</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">confirmed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">immunocytochemical</tok> <tok cat="NN">analysis</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@16-12-2010
1022422308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="JJ">hormone-mediated</tok> <tok cat="NN">survival</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="UCP" syn="COOD"><cons cat="ADJP" role="PRD"><tok cat="NN">concentration</tok> <tok cat="JJ">dependent</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">reaching</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">half-maximal</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="CD">10</tok> <tok cat="NN">nM</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">ER</tok> <tok cat="NN">antagonist</tok></cons> <cons cat="NP"><tok cat="NN">ICI</tok> <tok cat="CD">182</tok><tok cat="COMMA">,</tok><tok cat="CD">780</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">undifferentiated</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">further</tok></cons> <cons cat="VP"><tok cat="VBG">supporting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">receptor-mediated</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">survival</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
1022422309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="JJ">Other</tok> <tok cat="NN">steroid</tok> <tok cat="NN">receptor</tok> <tok cat="NNS">drugs</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Raloxifene</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">RU486</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ICI</tok> <tok cat="CD">182</tok><tok cat="COMMA">,</tok><tok cat="CD">780</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">PMA-differentiated</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">displayed</tok> <cons cat="NP"><tok cat="NN">agonist</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">preventing</tok> <cons cat="NP"><tok cat="JJ">TNF-alpha-induced</tok> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons> <cons cat="ADVP"><cons cat="ADVP"><tok cat="RB">as</tok> <tok cat="RB">efficiently</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">hormones</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">providing</tok> <cons cat="NP"><tok cat="JJ">further</tok> <tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">notion</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">steroid</tok> <tok cat="NN">receptor</tok> <tok cat="NNS">drugs</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">manifest</tok> <cons cat="NP"><tok cat="NN">agonist</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">antagonist</tok> <tok cat="NNS">activities</tok></cons> <cons cat="VP"><tok cat="VBG">depending</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">context</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i8"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">studied</tok><cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP" ref="i8" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@60@@oe@16-12-2010
1022422310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="NN">Treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">E2</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">time-dependent</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">proapoptotic</tok> <tok cat="NN">Nip-2</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">supporting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">hypothesis</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="NN">hormone</tok> <tok cat="NN">responsiveness</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">target</tok> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
1022422311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Together</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i12" role="SBJ"><cons cat="NP"><tok cat="NN">ER</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">PR</tok></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">endogenous</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">exogenous</tok></cons></cons> <tok cat="NNS">ligands</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">genetic</tok> <tok cat="NN">response</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i13"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i13" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">impairs</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">TNF-alpha-induced</tok> <tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@16-12-2010
1022422312@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBN">presented</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">here</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i14" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">female</tok> <tok cat="NN">steroid</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="VP"><tok cat="VBP">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i14" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">preventing</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">monoblastoid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">effect</tok></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">important</tok> <tok cat="NNS">consequences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">clinical</tok> <tok cat="NN">use</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">steroid</tok> <tok cat="NN">receptor</tok> <tok cat="NNS">drugs</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@16-12-2010
1022422313@GENIA Treebank@formal@@1@S@<cons cat="NP" syn="COOD"><tok cat="COLON">--</tok><cons cat="NP"><tok cat="NNP">Vegeto</tok><tok cat="COMMA">,</tok> <tok cat="NNP">E.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Pollio</tok><tok cat="COMMA">,</tok> <tok cat="NNP">G.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Pellicciari</tok><tok cat="COMMA">,</tok> <tok cat="NNP">C.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Maggi</tok><tok cat="COMMA">,</tok> <tok cat="NNP">A</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1022422314@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Estrogen</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">progesterone</tok></cons></cons> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="PP"><cons cat="NP"><tok cat="NN">survival</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">monoblastoid</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">undergoing</tok> <cons cat="NP"><tok cat="JJ">TNF-alpha-inuced</tok> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
1022427801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Grf40</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">novel</tok> <tok cat="NN">Grb2</tok> <tok cat="NN">family</tok> <tok cat="NN">member</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="VBN">involved</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">signaling</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">SLP-76</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">LAT</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@16-12-2010
1022427802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">molecularly</tok></cons> <cons cat="VP"><tok cat="VBD">cloned</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">new</tok> <tok cat="NN">Grb2</tok> <tok cat="NN">family</tok> <tok cat="NN">member</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">named</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NN">Grf40</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">common</tok> <tok cat="NN">SH3-SH2-SH3</tok> <tok cat="NN">motif</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1022427803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Grf40</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">predominant</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">particularly</tok></cons> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
1022427804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Grf40</tok></cons> <cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">SH2</tok> <tok cat="JJ">domain-containing</tok> <tok cat="NN">leukocyte</tok> <tok cat="NN">protein</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">76</tok> <tok cat="NN">kD</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SLP-76</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">SH3</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="ADVP"><cons cat="ADVP"><tok cat="RBR">more</tok> <tok cat="RB">tightly</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><tok cat="NN">Grb2</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1022427805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Incidentally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">Grf40</tok></cons> <cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">linker</tok> <tok cat="IN">for</tok> <tok cat="NN">activation</tok> <tok cat="IN">of</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LAT</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">possibly</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">SH2</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@16-12-2010
1022427806@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Overexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">wild-type</tok> <tok cat="NN">Grf40</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">significant</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">SLP-76-dependent</tok> <tok cat="NN">interleukin</tok> <tok cat="LRB">(</tok><tok cat="NN">IL</tok><tok cat="RRB">)</tok><tok cat="CD">-2</tok> <tok cat="NN">promoter</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="IN">of</tok> <tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="LRB">(</tok><tok cat="NN">NF-AT</tok><tok cat="RRB">)</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">receptor</tok> <tok cat="LRB">(</tok><tok cat="NN">TCR</tok><tok cat="RRB">)</tok> <tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">COOH-terminal</tok> <tok cat="JJ">SH3-deleted</tok> <tok cat="NN">Grf40</tok> <tok cat="NN">mutant</tok></cons> <cons cat="VP"><tok cat="VBD">lacked</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">any</tok> <tok cat="JJ">recognizable</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@54@@oe@16-12-2010
1022427807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">SH2-deleted</tok> <tok cat="NN">Grf40</tok> <tok cat="JJ">mutant</tok></cons> <cons cat="VP"><tok cat="VBD">led</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">marked</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">activities</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i1"><cons cat="WHNP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="WHPP"><tok cat="IN">of</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">apparently</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJR">stronger</tok> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">SH2-deleted</tok> <tok cat="NN">Grb2</tok> <tok cat="NN">mutant</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@16-12-2010
1022427808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Grf40</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">adaptor</tok> <tok cat="NN">molecule</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">TCR-mediated</tok> <tok cat="NN">signaling</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RBR">more</tok> <tok cat="JJ">efficient</tok></cons> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Grb2</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">SLP-76</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">LAT</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1022447001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Signal</tok> <tok cat="NN">transduction</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="NN">interferon-gamma</tok> <tok cat="NN">receptor</tok></cons></cons> <cons cat="NP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">eosinophils</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@16-12-2010
1022447002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP"><tok cat="NN">BACKGROUND</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">reported</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">constitutive</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interferon-gamma</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IFN-gammaR</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NNS">eosinophils</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1022447003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="CC">But</tok></cons> <cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="NN">IFN-gammaR</tok></cons></cons> <cons cat="NP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NNS">eosinophils</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">remains</tok> <cons cat="S" role="PRD"><cons cat="NP" ref="i1" id="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">elucidated</tok><cons cat="NP" ref="i2" null="NONE"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@16-12-2010
1022447004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Jak/Stat</tok> <tok cat="NN">pathway</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">signaling</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">eosinophils</tok></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IFN-gammaR</tok> <tok cat="NN">conjugation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ligand</tok> <tok cat="NN">binding</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@16-12-2010
1022447005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP"><tok cat="NNS">METHODS</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" id="i3" role="SBJ"><tok cat="VBN">Purified</tok> <tok cat="JJ">peripheral</tok> <tok cat="NNS">eosinophils</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="CD">37</tok> <tok cat="NNS">degrees</tok> <tok cat="NN">C</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="QP"><tok cat="CD">1-60</tok></cons> <tok cat="NN">min</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1022447006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="NN">Tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IFN-gammaR</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Jak1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Jak2</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Stat1alpha</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">immunoblotting</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@16-12-2010
1022447007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="JJ">Gel-shift</tok> <tok cat="NN">assay</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">show</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">formation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Stat1alpha-DNA</tok> <tok cat="NNS">complexes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@16-12-2010
1022447008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP"><tok cat="NNS">RESULTS</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">eosinophils</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">initiated</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">series</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">events</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">rapid</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="RB">not</tok> <tok cat="RB">only</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IFN-gammaRalpha</tok> <tok cat="NN">chain</tok></cons> <tok cat="CC">but</tok> <tok cat="RB">also</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Jak1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Jak2</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Stat1alpha</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@16-12-2010
1022447009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">IFN-gamma</tok></cons> <cons cat="VP"><tok cat="VBD">enhanced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Stat1alpha</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@16-12-2010
1022447010@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP"><tok cat="NN">CONCLUSION</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IFN-gamma</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">affects</tok> <cons cat="NP"><tok cat="NNS">eosinophils</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">specific</tok> <tok cat="NN">receptor</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">utilizes</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Jak/Stat</tok> <tok cat="NN">pathway</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">mode</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">signaling</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@16-12-2010
1022537701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappaB</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lipopolysaccharide</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NNS">leukocytes</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">prevented</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">inhibitors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">cytosolic</tok> <tok cat="NN">phospholipase</tok> <tok cat="NN">A2</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1022537702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">lipopolysaccharide</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">synthesis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">85-kDa</tok> <tok cat="JJ">cytosolic</tok> <tok cat="NN">phospholipase</tok> <tok cat="NN">A2</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1022537703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">enzyme</tok></cons> <cons cat="VP"><tok cat="VBZ">releases</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">arachidonic</tok> <tok cat="NN">acid</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">lyso-phospholipids</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">membranes</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" id="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">metabolized</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">eicosanoids</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">platelet-activating-factor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1022537704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NN">lipid</tok> <tok cat="NNS">mediators</tok></cons> <cons cat="VP"><tok cat="VBP">increase</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">cytosolic</tok> <tok cat="NN">phospholipase</tok> <tok cat="NN">A2</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">inflammation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1022537705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="ADVP"><tok cat="RB">previously</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">trifluoromethylketone</tok> <tok cat="NNS">inhibitors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">cytosolic</tok> <tok cat="NN">phospholipase</tok> <tok cat="NN">A2</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">suppressed</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">interleukin-1beta</tok> <tok cat="NN">protein</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">steady-state</tok> <tok cat="NN">mRNA</tok> <tok cat="NNS">levels</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">lipopolysaccharide-stimulated</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">leukocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@16-12-2010
1022537706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">subcellular</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="SBAR"><cons cat="WHPP" id="i6"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">trifluoromethylketones</tok></cons> <cons cat="VP"><tok cat="VBP">interfere</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons><cons cat="PP" ref="i6" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@16-12-2010
1022537707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBD">reduced</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">initial</tok> <tok cat="NN">interleukin-1beta</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">transcription</tok> <tok cat="NN">rate</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">prevention</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">degradation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">inhibitor-kappaB</tok> <tok cat="NN">alpha</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1022537708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Consequently</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">cytosolic</tok> <tok cat="NN">activation</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">translocation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">DNA-binding</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappaB</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">decreased</tok><cons cat="NP" ref="i7" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@16-12-2010
1022537709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">Trifluoromethylketones</tok></cons> <cons cat="VP"><tok cat="VBP">ameliorate</tok> <cons cat="NP"><tok cat="JJ">chronic</tok> <tok cat="NN">inflammation</tok></cons> <cons cat="ADVP"><tok cat="IN">in</tok> <tok cat="FW">vivo</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@7@@oe@16-12-2010
1022537710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="JJ">therapeutic</tok> <tok cat="NN">potency</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">reside</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">retention</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">inactive</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappaB</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytosol</tok></cons></cons></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">thereby</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">abrogating</tok> <cons cat="NP"><tok cat="NN">interleukin-1beta</tok> <tok cat="NN">gene</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@16-12-2010
1022597901@GENIA Treebank@formal@@1@S@<cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Constitutive</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">epithelial</tok> <tok cat="NN">signal</tok> <tok cat="NN">transducer</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">STAT</tok></cons><tok cat="RRB">)</tok></cons> <tok cat="NN">pathway</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">asthma</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@16-12-2010
1022597902@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Cytokine</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">immunity</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">inflammation</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">often</tok></cons> <cons cat="VP"><tok cat="VBP">depend</tok> <cons cat="NP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="VP"><tok cat="VBN">termed</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NNS">transducers</tok> <tok cat="CC">and</tok> <tok cat="NNS">activators</tok> <tok cat="IN">of</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">STATs</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="RB">so</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="NN">STAT</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="NNS">candidates</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">influencing</tok> <cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NN">disease</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
1022597903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">reasoned</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">selective</tok> <tok cat="NN">IFN</tok> <tok cat="NN">responsiveness</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NN">STAT</tok> <tok cat="NN">family</tok> <tok cat="NN">member</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Stat1</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Stat1-dependent</tok> <tok cat="JJ">immune-response</tok> <tok cat="NNS">genes</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">intercellular</tok> <tok cat="NN">adhesion</tok> <tok cat="NN">molecule-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ICAM-1</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IFN</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">factor-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IRF-1</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Stat1</tok></cons> <cons cat="NP"><tok cat="PRP">itself</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">airway</tok> <tok cat="JJ">epithelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">provides</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">basis</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">detecting</tok> <cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">abnormalities</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NN">airway</tok> <tok cat="NN">disease</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@55@@oe@16-12-2010
1022597904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">On</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">basis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">localization</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">phosphorylation</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="JJ">epithelial</tok> <tok cat="NN">Stat1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><tok cat="CC">but</tok> <tok cat="RB">not</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NN">control</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">invariably</tok></cons> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">asthmatic</tok></cons></cons> <cons cat="S"><cons cat="NP" ref="i2" id="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="JJ">control</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">chronic</tok> <tok cat="NN">bronchitis</tok> <tok cat="NNS">subjects</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@16-12-2010
1022597905@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="ADVP" role="PRD"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">epithelial</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">Stat1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">correlated</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">epithelial</tok> <tok cat="NN">ICAM-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IRF-1</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Stat1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">turn</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">ICAM-1</tok> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBD">correlated</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">accumulation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">tissue</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@16-12-2010
1022597906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i4" role="SBJ"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">low</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">IFN-gamma-producing</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">airway</tok> <tok cat="NN">tissue</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">all</tok> <tok cat="NNS">subjects</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@16-12-2010
1022597907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="ADVP" role="PRD"><tok cat="RB">therefore</tok></cons> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><cons cat="NP" id="i5"><tok cat="JJ">initial</tok> <tok cat="NN">evidence</tok></cons> <cons cat="VP"><tok cat="VBG">linking</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">abnormal</tok> <tok cat="NN">behavior</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NNS">pathways</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">signaling</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">development</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">inflammatory</tok> <tok cat="NN">disease</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@16-12-2010
1022597908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">that</tok> <tok cat="NN">context</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">results</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">change</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">current</tok> <tok cat="NN">scheme</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">asthma</tok> <tok cat="NN">pathogenesis</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">must</tok> <cons cat="VP"><tok cat="VB">include</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">localized</tok> <tok cat="NN">gain</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">signal</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">ordinarily</tok></cons> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">T</tok> <tok cat="NN">helper</tok> <tok cat="JJ">1-type</tok> <tok cat="NN">cytokine</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">allergy-driven</tok> <tok cat="NN">overproduction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">helper</tok> <tok cat="JJ">2-type</tok> <tok cat="NNS">cytokines</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@16-12-2010