820099801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Prenatal</tok> <tok cat="JJ">immune</tok> <tok cat="NN">challenge</tok></cons> <cons cat="VP"><tok cat="VBZ">alters</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">hypothalamic-pituitary-adrenocortical</tok> <tok cat="NN">axis</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="NNS">rats</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@11@@oe@19-12-2010
820099802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">non-abortive</tok></cons> <cons cat="ADJP"><tok cat="JJ">maternal</tok></cons></cons> <tok cat="NNS">infections</tok></cons> <cons cat="VP"><tok cat="MD">would</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">compromise</tok> <cons cat="NP"><tok cat="JJ">fetal</tok> <tok cat="NN">brain</tok> <tok cat="NN">development</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">alter</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">hypothalamic-pituitary-adrenocortical</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HPA</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">axis</tok></cons> <cons cat="VP"><tok cat="VBG">functioning</tok> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i5"><tok cat="WRB">when</tok></cons> <cons cat="FRAG"><cons cat="ADJP"><tok cat="JJ">adult</tok></cons></cons><cons cat="ADVP" ref="i5" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@22@@oe@19-12-2010
820099803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">study</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">putative</tok> <tok cat="JJ">teratogenic</tok></cons> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">T</tok> <tok cat="JJ">cell-mediated</tok> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons> <tok cat="CC">versus</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">endotoxic</tok> <tok cat="NN">challenge</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" role="SBJ"><tok cat="JJ">10-d-pregnant</tok> <tok cat="NNS">rats</tok></cons> <cons cat="VP"><tok cat="VBD">received</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="JJ">single</tok> <tok cat="JJ">intraperitoneal</tok></cons> <tok cat="NN">injection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="QP"><tok cat="CD">5</tok> <tok cat="CC">x</tok> <tok cat="CD">10(8)</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">red</tok> <tok cat="NN">blood</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HRBC</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">gram-negative</tok> <tok cat="JJ">bacterial</tok></cons> <tok cat="NN">endotoxin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">Escherichia</tok> <tok cat="FW">coli</tok></cons> <tok cat="NN">LPS</tok><tok cat="COLON">:</tok></cons> <cons cat="NP"><tok cat="CD">30</tok> <tok cat="NNS">micrograms/kg</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@19-12-2010
820099804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="ADJP"><tok cat="JJ">adult</tok> <tok cat="JJ">male</tok></cons> <tok cat="NN">progeny</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="ADJP"><cons cat="NP"><tok cat="CD">3</tok> <tok cat="NN">mo</tok></cons> <tok cat="JJ">old</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="JJ">experimental</tok> <tok cat="NNS">groups</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="JJ">basal</tok> <tok cat="NN">plasma</tok> <tok cat="NN">corticosterone</tok> <tok cat="NNS">levels</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
820099805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">novelty</tok> <tok cat="NN">stress</tok></cons></cons> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HRBC</tok> <tok cat="NN">group</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">LPS</tok> <tok cat="NN">group</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP" syn="COOD"><tok cat="VBN">increased</tok> <cons cat="NP"><tok cat="NN">ACTH</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">corticosterone</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">levels</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
820099806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">Both</tok> <tok cat="NNS">groups</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">substantial</tok> <tok cat="NNS">decreases</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">mineralocorticoid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MR</tok></cons><tok cat="RRB">)</tok></cons> <cons cat="NP" ref="i30" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GR</tok></cons><tok cat="RRB">)</tok></cons> <cons cat="NP" ref="i30" null="RNR"></cons></cons><cons cat="NP" id="i30"><tok cat="NNS">levels</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">hippocampus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">limbic</tok> <tok cat="NN">brain</tok> <tok cat="NN">structure</tok></cons> <cons cat="ADJP"><tok cat="JJ">critical</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">HPA</tok> <tok cat="NN">axis</tok> <tok cat="NN">regulation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP" id="i16"><tok cat="NN">GR</tok> <tok cat="NNS">concentrations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">hypothalamus</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unchanged</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" ref="i16" null="RNR"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">anterior</tok> <tok cat="NN">pituitary</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">slightly</tok></cons> <cons cat="VP"><tok cat="VBN">increased</tok><cons cat="NP" ref="i6" null="NONE"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@19-12-2010
820099807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">HRBC</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">LPS</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">indeed</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">stimulated</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">maternal</tok> <tok cat="JJ">immune</tok> <tok cat="NN">system</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">revealed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">specific</tok> <tok cat="JJ">anti-HRBC</tok> <tok cat="NN">antibody</tok> <tok cat="NN">production</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">enhanced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="NN">beta</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">splenocytes</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
820099808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBZ">demonstrates</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">T</tok> <tok cat="JJ">cell-mediated</tok> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">endotoxic</tok> <tok cat="NN">challenge</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">pregnancy</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><tok cat="NNS">anomalies</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HPA</tok> <tok cat="NN">axis</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">adulthood</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
820099809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Clinically</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">postulated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">disturbed</tok> <tok cat="JJ">fetal</tok> <tok cat="NN">brain</tok> <tok cat="NN">development</tok></cons> <cons cat="ADJP"><tok cat="JJ">due</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">prenatal</tok> <tok cat="JJ">immune</tok> <tok cat="NN">challenge</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">increases</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">vulnerability</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">develop</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">mental</tok> <tok cat="NN">illness</tok></cons> <cons cat="VP"><tok cat="VBG">involving</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">inadequate</tok> <tok cat="NNS">responses</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">stress</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
820562101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">JNK</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">integration</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">costimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@12@@oe@19-12-2010
820562102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-2</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">production</tok></cons></cons> <cons cat="VP"><tok cat="VBP">require</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok> <tok cat="CD">two</tok></cons> <tok cat="NNS">signals</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">generated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">ester</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TPA</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Ca2+</tok> <tok cat="NN">ionophore</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">costimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TCR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CD28</tok> <tok cat="NN">auxiliary</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@41@@oe@19-12-2010
820562103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="SBAR"><cons cat="WHADVP" id="i6"><tok cat="WRB">how</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">stimuli</tok></cons> <cons cat="VP"><tok cat="VBP">affect</tok> <cons cat="NP"><tok cat="NN">mitogen</tok> <tok cat="JJ">activated</tok> <tok cat="NN">protein</tok> <tok cat="LRB">(</tok><tok cat="NN">MAP</tok><tok cat="RRB">)</tok> <tok cat="NNS">kinases</tok></cons><cons cat="ADVP" ref="i6" null="T"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
820562104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Full</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">MAP</tok> <tok cat="NNS">kinases</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">phosphorylate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Jun</tok> <tok cat="NN">activation</tok> <tok cat="NN">domain</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">JNK1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">JNK2</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">required</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">costimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="NN">TPA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Ca2+</tok> <tok cat="NN">ionophore</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">antibodies</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">TCR</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CD28</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@19-12-2010
820562105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Alone</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">each</tok> <tok cat="NN">stimulus</tok></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">little</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">no</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">activation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@19-12-2010
820562106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="JJ">Similar</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">induction</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">cyclosporin</tok> <tok cat="NN">A</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CsA</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">synergistic</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">JNK</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">competitive</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Jun</tok> <tok cat="NN">phosphorylation</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">JNK</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@19-12-2010
820562107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i16" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">MAP</tok> <tok cat="NNS">kinases</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ERK1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">ERK2</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">fully</tok></cons> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i16" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">TPA</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">TCR</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">affected</tok> <cons cat="NP" ref="i16" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">Ca2+</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CD28</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">CsA</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
820562108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Hence</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">integration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">signals</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">lead</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">JNK</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
820675301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Glucocorticoid-mediated</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-2</tok> <tok cat="NN">receptor</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">alpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">-beta</tok></cons></cons> <tok cat="NN">subunit</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
820675302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">glucocorticoid</tok> <tok cat="LRB">(</tok><tok cat="NN">GC</tok><tok cat="RRB">)</tok><tok cat="JJ">-mediated</tok></cons> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">functions</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">dexamethasone</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">DM</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">proliferation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-2</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-2R</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">generation</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">studied</tok><cons cat="NP" ref="i8" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@19-12-2010
820675303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Dexamethasone</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="JJ">IL-2-induced</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">proliferation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="CD">30</tok><tok cat="NN">%</tok><tok cat="TO">-</tok><tok cat="CD">88</tok><tok cat="NN">%</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">relative</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">concentration</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cultures</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
820675304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DM</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Tac</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p55</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CD25</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" null="QSTN"></cons><tok cat="NN">beta</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">p75</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="NNS">genes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="ADVP" adv="TMP"><tok cat="RB">next</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
820675305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="VBN">purified</tok> <tok cat="NN">phytohemagglutinin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PHA-p</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">4</tok> <tok cat="NN">beta-phorbol</tok> <tok cat="NN">12-myristate</tok> <tok cat="NN">13-acetate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PMA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DM</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cultures</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="CD">60</tok><tok cat="NN">%</tok> <tok cat="NN">reduction</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="CD">30</tok><tok cat="NN">%</tok> <tok cat="NN">reduction</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">beta</tok> <tok cat="NN">membrane</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons> <cons cat="S"><cons cat="NP" ref="i10" id="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">cultured</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">absence</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DM</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">p</tok></cons> <cons cat="NP" role="PRD"><cons cat="QP"><tok cat="JJR">&lt;</tok> <tok cat="CD">0.01</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@59@@oe@19-12-2010
820675306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">membrane</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">beta</tok></cons></cons> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="CD">10(-6)</tok> <tok cat="NN">M</tok></cons> <tok cat="NN">DM</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">partially</tok> <tok cat="JJ">reversible</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">recombinant</tok> <tok cat="JJ">human</tok> <tok cat="NN">IL-2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">rhIL-2</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
820675307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="NP"><tok cat="JJ">Northern</tok> <tok cat="NN">blot</tok> <tok cat="NN">analysis</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">DM</tok></cons> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">comparable</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok> <cons cat="NP" ref="i12" null="RNR"></cons></cons></cons><tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">beta</tok> <cons cat="NP" ref="i12" null="RNR"></cons></cons></cons></cons><cons cat="NP" id="i12"><tok cat="NN">mRNA</tok> <tok cat="NNS">levels</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">membrane</tok> <tok cat="NN">receptor</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">mitogen-stimulated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
820675308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">transcription</tok> <tok cat="NNS">assays</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">DM</tok></cons> <cons cat="VP"><tok cat="VBD">regulated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">level</tok></cons></cons></cons> <cons cat="SBAR"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" id="i13" role="SBJ"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">beta</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">unaffected</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">DM</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
820675309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">action</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DM</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" ref="i14" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">determining</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mRNA</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">p50</tok> <tok cat="NN">subunit</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i15"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i15" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@19-12-2010
820675310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i16" role="SBJ"><cons cat="ADJP"><tok cat="CD">10(-6)</tok> <tok cat="NN">M</tok></cons> <tok cat="NN">DM</tok></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">p50</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">mRNA</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">four-fold</tok></cons></cons> <cons cat="S"><cons cat="NP" ref="i16" id="i17" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" ref="i17" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">absence</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DM</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
820675311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Further</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="ADJP"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NNS">sites</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">DM</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
820675312@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">sum</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">DM</tok></cons> <cons cat="VP"><tok cat="VBZ">regulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">membrane</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="QP"><tok cat="JJR">more</tok> <tok cat="IN">than</tok> <tok cat="CD">one</tok></cons> <tok cat="JJ">molecular</tok> <tok cat="NN">mechanism</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
821296501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">impaired</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">AP-1</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">subjects</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">some</tok> <tok cat="NNS">symptoms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">premature</tok> <tok cat="NN">aging</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
821296502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">study</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">disorders</tok></cons> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="JJ">premature</tok> <tok cat="NN">aging</tok> <tok cat="NNS">syndromes</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">insight</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NN">senescence</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
821296503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">main</tok> <tok cat="NN">feature</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NN">senescence</tok></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="NN">cessation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">proliferation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
821296504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i72" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Down</tok> <tok cat="NN">syndrome</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">DS</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">neuronal</tok> <tok cat="NN">ceroid-lypofuscinosis</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NCL</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">clinically</tok></cons> <tok cat="VBN">characterized</tok> <cons cat="NP" ref="i72" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">premature</tok> <tok cat="NN">onset</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">numerous</tok> <tok cat="NNS">features</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">normally</tok></cons> <tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">aging</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
821296505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="ADJP"><cons cat="NP"><tok cat="NN">Phytohemagglutinin</tok></cons> <tok cat="JJ">stimulated</tok></cons> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">DS</tok> <tok cat="NNS">subjects</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">statistically</tok> <tok cat="JJ">significant</tok></cons> <tok cat="VBN">diminished</tok> <tok cat="NN">proliferation</tok> <tok cat="NN">capacity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">comparison</tok> <tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="UCP" syn="COOD"><cons cat="NP"><tok cat="NN">NCL</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">healthy</tok></cons></cons> <tok cat="NNS">individuals</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
821296506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i73" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i73" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">applying</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="ADJP" id="i114"><tok cat="RB">slightly</tok> <tok cat="JJ">impaired</tok></cons> <tok cat="NN">AP-1</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP" id="i113"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">NCL</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons map="i114" cat="ADJP"><tok cat="JJ">strong</tok></cons> <cons map="i113" cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">DS</tok> <tok cat="NNS">ones</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
821296507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="JJ">molecular</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">proliferation</tok> <tok cat="NN">cessation</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">exist</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">fibroblasts</tok></cons> <cons cat="VP"><tok cat="VBN">characterized</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">replicative</tok> <tok cat="NN">senescence</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">individuals</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">premature</tok> <tok cat="NN">aging</tok> <tok cat="NNS">syndromes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Down</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
825418501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Visualization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">endogenous</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">p50</tok> <tok cat="NN">subunit</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">follicular</tok> <tok cat="JJ">dendritic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">germinal</tok> <tok cat="NNS">centers</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
825418502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><cons cat="ADJP"><tok cat="CD">50</tok> <tok cat="CD">kDa/65</tok> <tok cat="NN">kDa</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="ADJP"><tok cat="NN">p50/p65</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">heterodimer</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">ubiquitous</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">positive</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">various</tok> <tok cat="JJ">immune</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
825418503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">aim</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="S" role="PRD"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">related</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">particular</tok> <tok cat="NN">cell</tok> <tok cat="NN">type</tok></cons> <tok cat="CC">and/or</tok> <cons cat="NP"><tok cat="NN">differentiation</tok> <tok cat="NN">step</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">immunopoiesis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
825418504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" ref="i20" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">situ</tok></cons> <tok cat="NN">hybridization</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">sections</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">non</tok> <tok cat="NN">HIV</tok></cons> <tok cat="JJ">hyperplastic</tok> <tok cat="NN">lymph</tok> <tok cat="NNS">nodes</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i20" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i21" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">gene</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="CD">105</tok> <tok cat="NN">kDa</tok></cons> <tok cat="NN">precursor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p50</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">overexpressed</tok> <cons cat="NP" ref="i21" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">light</tok> <tok cat="NN">zone</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">germinal</tok> <tok cat="NNS">centers</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">network</tok> <tok cat="NN">aspect</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i22"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i22" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">suggested</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">follicular</tok> <tok cat="JJ">dendritic</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">FDC</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@52@@oe@19-12-2010
825418505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" tpc="TPC" id="i101"><tok cat="IN">By</tok> <cons cat="NP" role="LGS"><tok cat="NN">immunohistochemistry</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i23" role="SBJ"><tok cat="NN">p50</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i23" id="i24" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">cytoplasm</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">nucleus</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">FDC</tok></cons></cons></cons></cons><cons cat="PP" ref="i101" null="T"></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">confirming</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">FDC</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
825418506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i25" role="SBJ"><tok cat="NN">p50</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i25" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytoplasm</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">all</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@19-12-2010
825418507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">focused</tok> <cons cat="NP"><tok cat="PRPP">our</tok> <tok cat="NN">study</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">isolated</tok> <tok cat="NN">FDC</tok> <tok cat="NNS">clusters</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NNS">tonsils</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
825418508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">As</tok> <cons cat="S"><cons cat="NP" id="i26" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">showed</tok> <cons cat="NP" ref="i26" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">tissue</tok> <tok cat="NNS">sections</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">detected</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">p50</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">cytoplasm</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">nucleus</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">FDC</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
825418509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Nuclei</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">FDC</tok> <tok cat="NNS">clusters</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">negative</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@19-12-2010
825418510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">next</tok></cons> <cons cat="VP"><tok cat="VBD">studied</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p65</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Rel</tok> <tok cat="NN">protein</tok></cons></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">FDC</tok> <tok cat="NNS">clusters</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@19-12-2010
825418511@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i27" role="SBJ"><tok cat="NN">p65</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i27" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytoplasm</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">FDC</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">nuclei</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">negative</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
825418512@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i28" role="SBJ"><tok cat="NN">p65</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i28" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">nuclei</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">some</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@19-12-2010
825418513@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i29" role="SBJ"><tok cat="NN">c-Rel</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i29" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="RB">only</tok> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytoplasm</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">nucleus</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">cytoplasm</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">FDC</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
825418514@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBD">indicated</tok> <cons cat="SBAR"><tok cat="IN">that</tok><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">context</tok> <tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">T</tok> <tok cat="JJ">cell-dependent</tok></cons> <tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">immunopoiesis</tok></cons> <cons cat="VP"><tok cat="VBG">occurring</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">FDC</tok> <tok cat="NNS">clusters</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">p50</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">mainly</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">related</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">FDC</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">massive</tok> <tok cat="NN">overexpression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">nuclei</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i31" role="SBJ"><tok cat="NN">p65</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i31" null="NONE"></cons><cons cat="PP" id="i32"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="VBN">scattered</tok> <tok cat="NN">manner</tok></cons></cons> <cons cat="PP" id="i33"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">nuclei</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons map="i31" cat="NP" role="SBJ"><tok cat="NN">c-Rel</tok> <tok cat="NN">protein</tok></cons> <cons map="i32" cat="ADVP"><tok cat="RB">exclusively</tok></cons> <cons map="i33" cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytoplasm</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">FDC</tok> <tok cat="NNS">clusters</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@59@@oe@19-12-2010
825418515@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBD">suggested</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="NNS">subunits</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">c-Rel</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">roles</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">immunopoiesis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
825477201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">YB-1</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="JJ">lymphotropic</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">I</tok> <tok cat="JJ">basal</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
825477202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Sequences</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i27"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i27" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">control</tok> <cons cat="NP"><tok cat="JJ">basal</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">lymphotropic</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">I</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HTLV-I</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">likely</tok></cons> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">initiation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">maintenance</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">virus</tok> <tok cat="NN">replication</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
825477203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP" ref="i28" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">analyzed</tok> <cons cat="NP" ref="i28" null="RNR"></cons></cons></cons><cons cat="NP" id="i28"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">45-nucleotide</tok> <tok cat="NN">sequence</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="JJ">downstream</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">element</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP"><tok cat="NN">DRE</tok> <tok cat="CD">1</tok></cons><tok cat="RRB">]</tok></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">+195</tok></cons> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="CD">+240</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">boundary</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">R/U5</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i29"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i29" id="i30" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i30" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">HTLV-I</tok> <tok cat="JJ">basal</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@19-12-2010
825477204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">protein</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p37</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i31"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i31" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="VP"><tok cat="VBD">bound</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">DRE</tok> <tok cat="CD">1</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
825477205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">An</tok> <tok cat="NN">affinity</tok> <tok cat="NN">column</tok> <tok cat="NN">fraction</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="NN">p37</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">stimulated</tok> <cons cat="NP"><tok cat="NN">HTLV-I</tok> <tok cat="NN">transcription</tok></cons> <cons cat="ADVP"><tok cat="RB">approximately</tok> <tok cat="RB">12-fold</tok></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
825477206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">identification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cDNA</tok> <tok cat="NN">clone</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">15B-7</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">Jurkat</tok> <tok cat="NN">expression</tok> <tok cat="NN">library</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i32"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i32" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DRE</tok> <tok cat="CD">1</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
825477207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i33" role="SBJ"><cons cat="NP"><tok cat="NN">Binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cDNA</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">similarly</tok></cons> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="VBN">purified</tok> <tok cat="NN">Jurkat</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">decreased</tok> <cons cat="NP" ref="i33" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">introduction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">site-specific</tok> <tok cat="NNS">mutations</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DRE</tok> <tok cat="CD">1</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
825477208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">In</tok> <tok cat="FW">vitro</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">translation</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">15B-7</tok> <tok cat="NN">cDNA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">produced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i34"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i34" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">bound</tok> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HTLV-I</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">+195</tok></cons> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="CD">+240</tok></cons></cons> <tok cat="NN">oligonucleotide</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
825477209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">partial</tok> <tok cat="NN">cDNA</tok></cons> <cons cat="VP"><tok cat="VBZ">encodes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">protein</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i35"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i35" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">homologous</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">C-terminal</tok> <tok cat="CD">196</tok> <tok cat="NN">amino</tok> <tok cat="NNS">acids</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">36-kDa</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">YB-1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
825477210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Cotransfection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">YB-1</tok> <tok cat="NN">expression</tok> <tok cat="NN">plasmid</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">increases</tok> <cons cat="NP"><tok cat="NN">HTLV-I</tok> <tok cat="JJ">basal</tok> <tok cat="NN">transcription</tok></cons> <cons cat="ADVP"><tok cat="RB">approximately</tok> <tok cat="RB">14-fold</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
825477211@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">On</tok> <tok cat="DT">the</tok> <tok cat="NN">basis</tok> <tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">weight</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NNS">characteristics</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <tok cat="NN">transactivation</tok> <tok cat="NN">activity</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">previously</tok> <tok cat="VBN">identified</tok></cons> <cons cat="ADJP"><tok cat="NN">DRE</tok> <tok cat="JJ">1-binding</tok></cons> <tok cat="NN">protein</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p37</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="NN">YB-1</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
825516201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">Chlorinated</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">dibenzo-p-dioxins</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">dibenzofurans</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="JJ">immune</tok> <tok cat="NN">system</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@19-12-2010
825516202@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="LST"><tok cat="LS">1</tok><tok cat="PERIOD">.</tok></cons><cons cat="NP"><tok cat="NN">Blood</tok> <tok cat="NN">cell</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">volunteers</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">moderately</tok> <tok cat="VBN">increased</tok></cons> <tok cat="NN">body</tok> <tok cat="NNS">burdens</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@19-12-2010
825516203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" ref="i51" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">monoclonal</tok> <tok cat="NNS">antibodies</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">mAbs</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">flow</tok> <tok cat="NN">cytometry</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i51" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">studied</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">variety</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">surface</tok> <tok cat="NNS">receptors</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lymphocyte</tok> <tok cat="NNS">subpopulations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">workers</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">moderately</tok> <tok cat="VBN">increased</tok></cons> <tok cat="NN">body</tok> <tok cat="NNS">burdens</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">2,3,7,8-tetrachlorodibenzo-p-dioxin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TCDD</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">other</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">polychlorinated</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">dibenzo-p-dioxins</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">dibenzofurans</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PCDD/PCDF</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">International-Toxicity</tok> <tok cat="NN">Equivalencies</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">I-TE</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@52@@oe@19-12-2010
825516204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">hypothesis</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i52" null="ZERO"></cons><cons cat="S"><cons cat="NP" ref="i52" id="i53" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">tested</tok> <cons cat="NP" ref="i53" null="NONE"></cons></cons></cons></cons></cons></cons></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="SBAR" role="PRD"><tok cat="IN">whether</tok> <tok cat="CC">or</tok> <tok cat="RB">not</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">humans</tok></cons> <cons cat="VP"><tok cat="VBP">exhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">similar</tok> <tok cat="NN">susceptibility</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NNS">PCDDs/PCDFs</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <tok cat="NN">respect</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">surface</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">respond</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">small</tok> <tok cat="NNS">doses</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">2,3,7,8-tetrachlorodibenzo-p-dioxin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TCDD</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="FW">Callithrix</tok> <tok cat="FW">jacchus</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@19-12-2010
825516205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD" role="PRD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">helper-inducer</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">memory</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">CD4+CD45R0+CD45RA-CD29highCD11a+</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">CD20+</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cytotoxic</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">CD8+CD56+/CD57+</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
825516206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i55" role="SBJ"><cons cat="NP"><tok cat="CD">68</tok> <tok cat="NNS">triple-labellings</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NNS">mAbs</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">performed</tok> <cons cat="NP" ref="i55" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">each</tok> <tok cat="NN">volunteer</tok></cons></cons></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">possibly</tok></cons> <tok cat="VB">generate</tok> <cons cat="NP"><tok cat="JJ">further</tok> <tok cat="NNS">hypotheses</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
825516207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">evaluated</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" id="i56" role="SBJ"><cons cat="NP"><tok cat="DT">any</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">variables</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i56" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">biomarker</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">effects</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">class</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">compounds</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
825516208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">There</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">main</tok> <tok cat="NNS">goals</tok></cons><tok cat="COLON">:</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">1</tok><tok cat="RRB">)</tok></cons> <cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">evaluate</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">workers</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">moderately</tok> <tok cat="VBN">increased</tok></cons> <tok cat="NN">PCDD/PCDF-body</tok> <tok cat="NN">burden</tok></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><cons cat="QP"><tok cat="CD">25-140</tok></cons> <tok cat="NN">ppt</tok></cons> <tok cat="NN">TCDD</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="ADJP"><cons cat="QP"><tok cat="CD">104-522</tok></cons> <tok cat="NN">ppt</tok></cons> <tok cat="NN">I-TE</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">blood</tok> <tok cat="NN">fat</tok></cons></cons></cons><tok cat="RRB">]</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">exhibit</tok> <cons cat="NP"><tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">surface</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">white</tok> <tok cat="NN">blood</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i57" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i57" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">previous</tok> <tok cat="NNS">studies</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">non-human</tok> <tok cat="NNS">primates</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">2</tok><tok cat="RRB">)</tok></cons> <cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">clarify</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" id="i102" role="SBJ"><cons cat="NP"><tok cat="NNS">persons</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">upper</tok> <tok cat="NN">range</tok></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><cons cat="QP"><tok cat="CD">10-23</tok></cons> <tok cat="NN">ppt</tok></cons> <tok cat="NN">TCDD</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="ADJP"><cons cat="QP"><tok cat="CD">30-90</tok></cons> <tok cat="NN">ppt</tok></cons> <tok cat="NN">I-TE</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">blood</tok> <tok cat="NN">fat</tok></cons></cons></cons><tok cat="RRB">]</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">body</tok> <tok cat="NN">burden</tok> <tok cat="NN">reference</tok> <tok cat="NNS">values</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><cons cat="ADVP"><tok cat="RB">not</tok> <tok cat="RB">particularly</tok></cons> <tok cat="VBN">exposed</tok></cons> <tok cat="NN">population</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="NP"><tok cat="JJ">detectable</tok> <tok cat="NNS">deviations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="JJ">immunological</tok> <tok cat="NNS">variables</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i58"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" ref="i102" id="i103" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" ref="i103" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">persons</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJR">lower</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="NN">medium</tok></cons></cons> <tok cat="NN">range</tok></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><cons cat="QP"><tok cat="CD">1-3</tok></cons> <tok cat="NN">ppt</tok></cons> <tok cat="NN">TCDD</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="ADJP"><cons cat="QP"><tok cat="CD">9-29</tok></cons> <tok cat="NN">ppt</tok></cons> <tok cat="NN">I-TE</tok></cons></cons><tok cat="RRB">]</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NN">body</tok> <tok cat="NN">burden</tok> <tok cat="NN">reference</tok> <tok cat="NNS">values</tok></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i58" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@121@@oe@19-12-2010
825516209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Regression</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">our</tok> <tok cat="NNS">data</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">slight</tok> <tok cat="NNS">trends</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">some</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">biomarkers</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="ADVP"><tok cat="FW">e.g.</tok></cons> <tok cat="JJ">CD45R0+</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
825516210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">With</tok> <cons cat="NP"><tok cat="CD">one</tok> <tok cat="NN">exception</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="DT">all</tok></cons> <cons cat="NP" role="PRD"><tok cat="NNS">increases</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@19-12-2010
825516211@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">None</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">alterations</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" null="NONE"></cons></cons></cons></cons></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="PP" role="PRD"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">medical</tok> <tok cat="NN">relevance</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@19-12-2010
825516212@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">slight</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">percentage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">CD4+CD45R0+</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">remained</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">significant</tok></cons> <cons cat="PP" adv="TMP"><tok cat="RB">even</tok> <tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">covariant</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBG">taking</tok> <cons cat="NP"><tok cat="JJ">age-related</tok> <tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="NN">account</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
825516213@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Altogether</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">do</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">any</tok> <tok cat="NN">evidence</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i59" null="ZERO"></cons><cons cat="S"><cons cat="NP" ref="i59" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">support</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">assumption</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">moderately</tok> <tok cat="VBN">increased</tok></cons> <tok cat="NN">body</tok> <tok cat="NNS">burdens</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">PCDDs/PCDFs</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">adults</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">decreases</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cellular</tok> <tok cat="NNS">components</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="JJ">immune</tok> <tok cat="NN">system</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@19-12-2010
825516214@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Adult</tok> <tok cat="NNS">humans</tok></cons> <cons cat="ADVP"><tok cat="RB">certainly</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="RBR">less</tok> <tok cat="JJ">susceptible</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">action</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">PCDDs/PCDFs</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><tok cat="JJ">adolescent</tok> <tok cat="FW">Callithrix</tok> <tok cat="FW">jacchus</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
826247701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Transient</tok> <tok cat="NN">pseudohypoaldosteronism</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">obstructive</tok> <tok cat="JJ">renal</tok> <tok cat="NN">disease</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">reduction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">lymphocytic</tok> <tok cat="NN">aldosterone</tok> <tok cat="NNS">receptors</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
826247702@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">Results</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">affected</tok> <tok cat="NNS">infants</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@6@@oe@19-12-2010
826247703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">pseudohypoaldosteronism</tok></cons> <cons cat="PP"><tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">obstructive</tok> <tok cat="JJ">renal</tok> <tok cat="NN">disease</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@19-12-2010
826247704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">Both</tok> <tok cat="NNS">patients</tok></cons> <cons cat="VP"><tok cat="VBD">presented</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">salt-losing</tok> <tok cat="NN">episode</tok> <tok cat="VBG">simulating</tok> <tok cat="JJ">adrenal</tok> <tok cat="NN">insufficiency</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@19-12-2010
826247705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="CD">one</tok> <tok cat="NN">patient</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i90" role="SBJ"><cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">reduction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">aldosterone</tok> <tok cat="NNS">receptors</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">documented</tok><cons cat="NP" ref="i90" null="NONE"></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">while</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">second</tok> <tok cat="NN">patient</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">clinical</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">biochemical</tok></cons></cons> <tok cat="NNS">parameters</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">pseudohypoaldosteronism</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
826247706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i91" role="SBJ"><tok cat="NN">Aldosterone</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">normal</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">patients</tok></cons></cons> <cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i92"><tok cat="IN">when</tok></cons> <cons cat="S"><cons cat="NP" ref="i91" id="i93" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">studied</tok> <cons cat="NP" ref="i93" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">surgical</tok> <tok cat="NN">correction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">obstruction</tok></cons></cons></cons></cons><cons cat="ADVP" ref="i92" null="T"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
828280301@GENIA Treebank@formal@@1@S@<cons cat="NP"><tok cat="JJ">Steroid-resistant</tok> <tok cat="NN">asthma</tok><tok cat="PERIOD">.</tok></cons>@@@@1@3@@oe@19-12-2010
828280302@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Cellular</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="VP"><tok cat="VBG">contributing</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">inadequate</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">therapy</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@19-12-2010
828280303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">current</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">alterations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GR</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">binding</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">poor</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">therapy</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">asthma</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
828280304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i19" role="SBJ"><cons cat="NP"><tok cat="CD">29</tok> <tok cat="NN">asthma</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="VBN">forced</tok> <tok cat="JJ">expiratory</tok> <tok cat="NN">volume</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="CD">1</tok> <tok cat="NN">s</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">FEV1</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP"><cons cat="QP"><tok cat="JJR">&lt;</tok> <tok cat="CD">70</tok></cons><tok cat="NN">%</tok></cons></cons> <cons cat="VP"><tok cat="VBN">predicted</tok> <cons cat="NP" null="NONE"></cons></cons></cons></cons></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">studied</tok><cons cat="NP" ref="i19" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
828280305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i20" role="SBJ"><tok cat="NNS">Patients</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">classified</tok> <cons cat="NP" ref="i20" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="ADJP" id="i102"><cons cat="ADJP"><tok cat="NN">steroid</tok> <tok cat="JJ">sensitive</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="ADJP"><tok cat="JJ">SS</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="SBAR" id="i103"><tok cat="IN">if</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">their</tok> <tok cat="NN">morning</tok> <tok cat="NN">FEV1</tok></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><cons cat="QP"><tok cat="RBR">&gt;</tok> <tok cat="CD">30</tok></cons><tok cat="NN">%</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">1-wk</tok> <tok cat="NN">course</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">oral</tok> <tok cat="NN">prednisone</tok></cons> <cons cat="NP"><tok cat="CD">20</tok> <tok cat="NN">mg</tok></cons> <cons cat="ADVP"><tok cat="RB">twice</tok> <tok cat="RB">daily</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons map="i102" cat="ADJP"><cons cat="ADJP"><tok cat="NN">steroid</tok> <tok cat="JJ">resistant</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="ADJP"><tok cat="JJ">SR</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons map="i103" cat="SBAR"><tok cat="IN">if</tok> <cons cat="S"><cons cat="NP" id="i21" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBD">failed</tok> <cons cat="S"><cons cat="NP" ref="i21" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">increase</tok> <cons cat="NP"><cons cat="QP"><tok cat="RBR">&gt;</tok> <tok cat="CD">15</tok></cons><tok cat="NN">%</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@19-12-2010
828280306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i22" role="SBJ"><cons cat="NP"><tok cat="NN">PBMC</tok></cons> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">these</tok> <tok cat="CD">two</tok> <tok cat="NNS">groups</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">17</tok> <tok cat="JJ">SR</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">12</tok> <tok cat="JJ">SS</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><tok cat="CD">12</tok> <tok cat="JJ">normal</tok> <tok cat="NNS">controls</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok><cons cat="NP" ref="i22" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
828280307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">SR</tok> <tok cat="NNS">patients</tok></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">distinguishable</tok> <tok cat="NN">GR</tok> <tok cat="NN">binding</tok> <tok cat="NNS">abnormalities</tok></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" id="i104" role="SBJ"><cons cat="NP"><tok cat="CD">15</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">17</tok> <tok cat="JJ">SR</tok> <tok cat="NNS">patients</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">significantly</tok> <tok cat="VBN">reduced</tok></cons> <tok cat="NN">GR</tok> <tok cat="NN">binding</tok> <tok cat="NN">affinity</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" ref="i104" id="i105" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" ref="i105" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">SS</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">0.0001</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NNS">controls</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">0.0001</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@19-12-2010
828280308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i23" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">defect</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="JJ">localized</tok> <cons cat="NP" ref="i23" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">reverted</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">normal</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">48</tok> <tok cat="NN">h</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">culture</tok> <tok cat="NNS">media</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
828280309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">incubation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">sustained</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">abnormality</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
828280310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">other</tok> <tok cat="CD">two</tok> <tok cat="JJ">SR</tok> <tok cat="NNS">patients</tok></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP"><tok cat="RB">abnormally</tok> <tok cat="JJ">low</tok></cons> <tok cat="NN">GR</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">binding</tok> <tok cat="NN">affinity</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i24"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i24" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="VBN">limited</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
828280311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i26" role="SBJ"><tok cat="NN">GR</tok> <tok cat="NN">number</tok></cons> <cons cat="VP"><tok cat="VBD">failed</tok> <cons cat="S"><cons cat="NP" ref="i26" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">normalize</tok> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">incubation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NNS">media</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
828280312@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="JJ">SR</tok> <tok cat="NN">asthma</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="PP" role="PRD"><tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="JJR">more</tok> <tok cat="IN">than</tok> <tok cat="CD">one</tok></cons> <tok cat="NN">abnormality</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">majority</tok></cons> <cons cat="ADJP"><tok cat="JJ">related</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">reversible</tok> <tok cat="JJ">cytokine-induced</tok> <tok cat="NN">reduction</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">GR</tok> <tok cat="NN">binding</tok> <tok cat="NN">affinity</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">second</tok></cons> <cons cat="ADJP"><tok cat="JJ">related</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">irreversible</tok> <tok cat="NN">reduction</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">GR</tok> <tok cat="NN">number</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@19-12-2010
828280313@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><tok cat="JJ">important</tok> <tok cat="NNS">implications</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">design</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">alternative</tok> <tok cat="NN">treatment</tok> <tok cat="NNS">approaches</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">recalcitrant</tok> <tok cat="NN">asthma</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
828282001@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">Rhabdomyosarcomas</tok></cons> <cons cat="VP"><tok cat="VBP">do</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">contain</tok> <cons cat="NP"><tok cat="NNS">mutations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NNS">domains</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">myogenic</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
828282002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i22" role="SBJ"><tok cat="JJ">Skeletal</tok> <tok cat="NN">myogenesis</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i22" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">group</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">MyoD</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">myogenin</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">myf5</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">myf6</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i23"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i23" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="LQT">"</tok><tok cat="JJ">basic</tok> <tok cat="JJ">helix-loop-helix</tok><tok cat="RQT">"</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i24"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i24" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">promoters</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">muscle-specific</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">promote</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@19-12-2010
828282003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">mutation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Leu122</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">Arg</tok></cons></cons></cons></cons> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="JJ">basic</tok> <tok cat="NN">domain</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MyoD</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">confers</tok> <cons cat="NP"><tok cat="JJ">c-myc-like</tok> <tok cat="JJ">functional</tok> <tok cat="NNS">characteristics</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
828282004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">used</tok> <cons cat="NP" id="i25"><tok cat="JJ">single-strand</tok> <tok cat="NN">conformation</tok> <tok cat="NN">polymorphism</tok> <tok cat="NN">analysis</tok></cons> <cons cat="S"><cons cat="NP" ref="i25" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">such</tok> <tok cat="NNS">mutations</tok></cons> <cons cat="VP"><tok cat="VBP">occur</tok> <cons cat="ADVP"><tok cat="RB">naturally</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">rhabdomyosarcomas</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
828282005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">basic</tok> <tok cat="NNS">domains</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PDT">all</tok> <tok cat="DT">the</tok> <tok cat="JJ">myogenic</tok> <tok cat="NNS">factors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">remain</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unaltered</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">rhabdomyosarcomas</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
828282006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Selection</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="JJ">such</tok> <tok cat="NNS">mutations</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">result</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="NN">redundancy</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="JJ">myogenic</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
828303201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="VBN">Increased</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">proliferation</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">cytotoxicity</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">surface</tok> <tok cat="NN">ganglioside</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GD3</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="VBN">published</tok> <tok cat="NN">erratum</tok></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNP">J</tok> <tok cat="NNP">Immunol</tok></cons> <cons cat="NP" adv="TMP"><tok cat="CD">1994</tok> <tok cat="NNP">Jul</tok> <tok cat="CD">15</tok></cons><tok cat="COLON">;</tok><cons cat="NP"><tok cat="CD">153</tok><tok cat="LRB">(</tok><tok cat="CD">2</tok><tok cat="RRB">)</tok></cons><tok cat="COLON">:</tok><cons cat="NP"><tok cat="CD">910</tok></cons></cons></cons></cons></cons><tok cat="RRB">]</tok></cons></cons>@@@@1@41@@oe@19-12-2010
828303202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Previous</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">suggested</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">gangliosides</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">cell</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">signaling</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">recognition</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
828303203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i13" role="SBJ"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">specific</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">processes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="ADVP"><tok cat="RB">clearly</tok></cons> <cons cat="VP"><tok cat="VBN">defined</tok><cons cat="NP" ref="i13" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
828303204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">mAb</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">R24</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i15"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i15" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">reacts</tok> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <tok cat="NN">ganglioside</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GD3</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="S"><cons cat="NP" ref="i14" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">stimulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">proliferation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
828303205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i16"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">R24</tok> <tok cat="NN">mAb</tok></cons> <cons cat="VP"><tok cat="VBZ">affects</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">functions</tok></cons><cons cat="PP" ref="i16" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
828303206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">R24</tok> <tok cat="NN">mAb</tok></cons> <cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">GD3+</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">proliferation</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">cytotoxicity</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">surface</tok> <tok cat="NN">marker</tok> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha-chain</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">beta-chain</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">HLA-DR</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CD11a</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CD11c</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
828303207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Additionally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">IFN-gamma</tok> <tok cat="NN">activity</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons> <tok cat="NN">activity</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">culture</tok> <tok cat="NNS">supernatants</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><cons cat="NP"><tok cat="CD">72</tok> <tok cat="NN">h</tok></cons> <tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">R24</tok> <tok cat="NN">stimulation</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
828303208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">some</tok> <tok cat="NNS">donors</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i17" role="SBJ"><tok cat="VBN">increased</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-6</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok></cons></cons> <tok cat="NN">activity</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i17" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">R24</tok> <tok cat="NN">treatment</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
828303209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">R24</tok> <tok cat="NN">treatment</tok></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-rel</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">little</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="DT">no</tok></cons></cons> <tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p50</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytoplasm</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">binding</tok></cons> <tok cat="NNS">complexes</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-rel</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p50</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@19-12-2010
828303210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">treatment</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">protein</tok> <tok cat="NNS">substrates</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@19-12-2010
828303211@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i18" role="SBJ"><cons cat="NP"><tok cat="JJ">R24-stimulated</tok> <tok cat="NNS">increases</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">proliferation</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">cytotoxicity</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <tok cat="NN">protein</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i18" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">cyclosporin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">staurosporin</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i20" role="SBJ"><cons cat="NP"><tok cat="NN">cyclophilin/calcineurin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i20" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">R24</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@19-12-2010
828303212@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Additionally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">herbimycin</tok> <tok cat="NN">A</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok> <tok cat="NN">inhibitor</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">blocked</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">R24-stimulated</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">proliferation</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">cytotoxicity</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">concentrations</tok></cons> <cons cat="ADJP"><tok cat="JJ">consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">specificity</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NNS">kinases</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
828303213@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i21" role="SBJ"><tok cat="JJ">multiple</tok> <tok cat="JJ">biochemical</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i21" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">R24</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
828947401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">SCL</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBZ">displays</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cell-specific</tok> <tok cat="NN">heterogeneity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">size</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@19-12-2010
828947402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i19" role="SBJ"><tok cat="NN">SCL</tok> <tok cat="NN">protein</tok> <tok cat="NN">production</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" ref="i19" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">variety</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">hemopoietic</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">immunoblotting</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="JJ">polyclonal</tok> <tok cat="NNS">antisera</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
828947403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i20" role="SBJ"><tok cat="NN">SCL</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i20" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="UCP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">erythroid</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">megakaryocyte</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">mast</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">early</tok> <tok cat="JJ">myeloid</tok></cons></cons> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="JJ">lymphoid</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i21"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i21" id="i22" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" ref="i22" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">harbor</tok> <cons cat="NP"><tok cat="NN">SCL</tok> <tok cat="NN">gene</tok> <tok cat="NNS">rearrangements</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@19-12-2010
828947404@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJS">most</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i23" role="SBJ"><cons cat="NP"><tok cat="NNS">proteins</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">molecular</tok> <tok cat="NN">weight</tok></cons> <cons cat="NP"><cons cat="QP"><tok cat="CD">49</tok> <tok cat="CC">and</tok> <tok cat="CD">44</tok></cons> <tok cat="NN">kDa</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">found</tok><cons cat="NP" ref="i23" null="NONE"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="ADVP"><tok cat="RB">however</tok></cons> <cons cat="NP" role="SBJ"><tok cat="CD">two</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP"><cons cat="NP"><tok cat="RB">only</tok> <tok cat="JJR">lower</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">weight</tok> <tok cat="NNS">species</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="QP"><tok cat="CD">24</tok> <tok cat="CC">and</tok> <tok cat="CD">22</tok></cons> <tok cat="NN">kDa</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
828947405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i24" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">size</tok> <tok cat="NN">discrepancy</tok></cons> <cons cat="VP"><tok cat="VBD">appeared</tok> <cons cat="S"><cons cat="NP" ref="i24" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="PP" role="PRD"><tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">cell-specific</tok> <tok cat="JJ">translational</tok> <tok cat="NN">regulation</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">since</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">overexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">retrovirally</tok> <tok cat="VBN">transfected</tok></cons> <tok cat="NN">SCL</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">yielded</tok> <cons cat="NP" id="i25"><tok cat="DT">the</tok> <tok cat="JJR">higher</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">weight</tok> <tok cat="NNS">forms</tok></cons> <cons cat="PP" id="i26"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJS">most</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">GP+E-86</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">AT2.5</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">M1</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><cons map="i25" cat="NP"><tok cat="RB">only</tok> <tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="CD">22</tok> <tok cat="NN">kDa</tok></cons> <tok cat="NN">form</tok></cons> <cons map="i26" cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">WEHI-3B/D+</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@19-12-2010
828947406@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Overexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">full-length</tok> <tok cat="NN">SCL</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">lymphoid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">SupT1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Raji</tok></cons></cons><tok cat="COMMA">,</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">alter</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">phenotype</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">autoregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SCL</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
828947407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">restricted</tok> <tok cat="NN">pattern</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SCL</tok> <tok cat="NN">protein</tok> <tok cat="NN">synthesis</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">restricted</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SCL</tok> <tok cat="NN">mRNA</tok></cons></cons> <cons cat="VP"><tok cat="VBN">documented</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
828947408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i27" role="SBJ"><tok cat="NN">SCL</tok> <tok cat="NN">protein</tok> <tok cat="NN">size</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">determined</tok> <cons cat="NP" ref="i27" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">translation</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">cell-specific</tok> <tok cat="NN">manner</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
828981301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="JJ">Human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">I</tok> <tok cat="NN">Tax</tok></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">associates</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" ref="i12" null="RNR"></cons></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">negatively</tok></cons> <tok cat="VBN">regulated</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" ref="i12" role="LGS" null="RNR"></cons></cons></cons></cons></cons><cons cat="NP" id="i12"><tok cat="DT">the</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B2</tok> <tok cat="NN">p100</tok> <tok cat="NN">gene</tok> <tok cat="NN">product</tok></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NNS">implications</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">latency</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@26@@oe@19-12-2010
828981302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">I</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HTLV-I</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">etiologic</tok> <tok cat="NN">agent</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">adult</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">aggressive</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><cons cat="ADVP" adv="TMP"><tok cat="RB">often</tok></cons> <tok cat="JJ">fatal</tok></cons></cons> <tok cat="NN">malignancy</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="JJ">human</tok> <tok cat="NN">CD4</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
828981303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HTLV-I</tok></cons> <cons cat="VP"><tok cat="VBZ">encodes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">essential</tok> <tok cat="JJ">40-kDa</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBN">termed</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="NN">Tax</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i16"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i16" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="CONJP"><tok cat="RB">not</tok> <tok cat="RB">only</tok></cons> <cons cat="VP"><tok cat="VBZ">transactivates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">retrovirus</tok></cons></cons></cons></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">array</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
828981304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Tax-mediated</tok> <tok cat="NN">transformation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">likely</tok></cons> <cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">deregulated</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">various</tok> <tok cat="JJ">cellular</tok> <tok cat="NNS">genes</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i15"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i15" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">normally</tok></cons> <cons cat="VP"><tok cat="VBP">regulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lymphocyte</tok> <tok cat="NN">growth</tok></cons> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">altered</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">various</tok> <tok cat="JJ">endogenous</tok> <tok cat="NN">host</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
828981305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">particular</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i13" role="SBJ"><tok cat="NN">Tax</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S"><cons cat="NP" ref="i13" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">modulating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">expression</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">activity</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NN">host</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B/Rel</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CREB/ATF</tok></cons></cons> <tok cat="NNS">families</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cellular</tok> <tok cat="NNS">factors</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HEB-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p67SRF</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@19-12-2010
828981306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">An</tok> <tok cat="JJ">additional</tok> <tok cat="JJ">distinguishing</tok> <tok cat="NN">characteristic</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HTLV-I</tok> <tok cat="NN">infection</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">profound</tok> <tok cat="NN">state</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">latency</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i14"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i14" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBG">circulating</tok> <tok cat="JJ">primary</tok> <tok cat="JJ">leukemic</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
828981307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HTLV-I</tok> <tok cat="NN">Tax</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">physically</tok></cons> <tok cat="VB">associate</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">p100</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">product</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">Rel-related</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B2</tok> <tok cat="NN">gene</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons> <cons cat="PP" syn="COOD"><tok cat="CC">both</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">transfected</tok> <tok cat="NNS">cells</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">HTLV-I-infected</tok> <tok cat="JJ">leukemic</tok> <tok cat="NN">T-cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@19-12-2010
828981308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">physical</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Tax</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">p100</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Tax-induced</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HTLV-I</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok></cons></cons> <tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NNS">repeats</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">reflecting</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">p100-mediated</tok> <tok cat="JJ">cytoplasmic</tok> <tok cat="NN">sequestration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><cons cat="ADVP"><tok cat="RB">normally</tok></cons> <tok cat="RB">nuclearly</tok> <tok cat="VBN">expressed</tok></cons> <tok cat="NN">Tax</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@19-12-2010
828981309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">mutant</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Tax</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i18"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i18" id="i19" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">selectively</tok></cons> <cons cat="VP"><tok cat="VBZ">fails</tok> <cons cat="S"><cons cat="NP" ref="i19" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">associate</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">p100</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
828981310@GENIA Treebank@formal@@1@S@<cons cat="S"><tok cat="LRB">(</tok><cons cat="NP" id="i20" role="SBJ"><tok cat="NN">ABSTRACT</tok></cons> <cons cat="VP"><tok cat="VBN">TRUNCATED</tok> <cons cat="NP" ref="i20" null="NONE"></cons><cons cat="PP"><tok cat="IN">AT</tok> <cons cat="NP"><tok cat="CD">250</tok> <tok cat="NNS">WORDS</tok></cons></cons></cons><tok cat="RRB">)</tok></cons>@@@@1@7@@oe@19-12-2010
829122801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">BZLF1</tok> <tok cat="NN">promoter</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">second</tok> <tok cat="NNS">messengers</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">anti-immunoglobulin-treated</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
829122802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Initiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EBV</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="JJ">lytic</tok> <tok cat="NN">cycle</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">BZLF1</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
829122803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">BZLF1</tok> <tok cat="NN">gene</tok> <tok cat="NN">promoter</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Zp</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">crosslinking</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">immunoglobulin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Ig</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">anti-Ig</tok> <tok cat="NN">antibody</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="RB">even</tok> <tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">absence</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="JJ">viral</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@19-12-2010
829122804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="JJ">anti-Ig</tok> <tok cat="NN">response</tok> <tok cat="NNS">elements</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="NN">Zp</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i11"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i11" id="i12" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">originally</tok></cons> <tok cat="VBN">defined</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">12-O-tetradecanoylphorbol-13-acetate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TPA</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">response</tok> <tok cat="NNS">elements</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ZI</tok> <tok cat="NNS">repeats</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">ZII</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">AP-1-like</tok> <tok cat="NN">domain</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
829122805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">Since</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">anti-Ig</tok> <tok cat="NN">crosslinking</tok></cons> <cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PKC</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">calcium</tok> <tok cat="NN">level</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i13" role="SBJ"><tok cat="NN">Zp</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">tested</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="JJ">cellular</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
829122806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">calcium</tok> <tok cat="NN">ionophore</tok> <tok cat="NN">A23187</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><tok cat="NN">Zp</tok> <tok cat="NN">activity</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@19-12-2010
829122807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i14"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" id="i15" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">calcium</tok> <tok cat="NN">ionophore</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">conjunction</tok> <tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">TPA</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">PKC</tok> <tok cat="NN">activator</tok></cons></cons></cons><cons cat="ADVP" ref="i14" null="T"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i16" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">Zp</tok> <tok cat="NN">induction</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">synergistically</tok></cons> <cons cat="VP"><tok cat="VBN">enhanced</tok><cons cat="NP" ref="i16" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
829122808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">1-(5-Isoquinolinyl sulfonyl)-2-methylpiperazine</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PKC</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">anti-Ig</tok> <tok cat="NN">inducibility</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Zp</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
829122809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Calmodulin</tok> <tok cat="NNS">antagonists</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">compound</tok> <tok cat="NN">R24571</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">trifluoperazine</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">blocked</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Zp</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">anti-Ig</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
829122810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Zp</tok></cons> <cons cat="VP"><tok cat="VBZ">responds</tok> <cons cat="ADVP"><tok cat="RB">directly</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">PKC</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">calcium/calmodulin-dependent</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
829122811@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i17" role="SBJ"><cons cat="NP"><tok cat="NN">Requirement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok> <tok cat="NN">activation</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">anti-Ig-mediated</tok> <tok cat="NN">Zp</tok> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">also</tok></cons> <tok cat="VBN">demonstrated</tok> <cons cat="NP" ref="i17" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">use</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok> <tok cat="NN">inhibitor</tok> <tok cat="NN">herbimycin</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
829122812@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">These</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">gene</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">molecules</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">anti-Ig</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">cooperatively</tok></cons> <tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">part</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">achieving</tok> <cons cat="NP"><tok cat="JJ">efficient</tok> <tok cat="NN">EBV</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons> <cons cat="SBAR"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i18" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">seen</tok> <cons cat="NP" ref="i18" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">anti-Ig</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
830229601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">phosphatidylinositol</tok> <tok cat="NN">turnover</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">EGR-1</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">crosslinking</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">surface</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IgM</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IgD</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">B104</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
830229602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NN">lymphoma</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">B104</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">surface</tok> <tok cat="NN">IgM</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">sIgM</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">surface</tok> <tok cat="NN">IgD</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">sIgD</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="VBG">crosslinking</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">sIgM</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">sIgD</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">anti-IgM</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">antibody</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Ab</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">anti-IgD</tok> <tok cat="NN">Ab</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><tok cat="NN">Ca2+</tok> <tok cat="NN">influx</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">almost</tok> <tok cat="DT">the</tok> <tok cat="JJ">same</tok></cons> <tok cat="NN">degree</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="RB">only</tok> <tok cat="NN">sIgM-crosslinking</tok></cons> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><tok cat="NN">B104</tok> <tok cat="NN">cell</tok> <tok cat="NN">death</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@63@@oe@19-12-2010
830229603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">accumulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cyclic</tok> <tok cat="NN">AMP</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">cAMP</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">hydrolysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">inositol</tok> <tok cat="NNS">phosphates</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PKC</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">activity</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Egr-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-fos</tok></cons></cons> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">sIgM-</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">sIgD-crosslinking</tok></cons></cons></cons></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">examine</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">differences</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">signals</tok></cons> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">sIgM</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">sIgD</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B104</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@54@@oe@19-12-2010
830229604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="CC">Both</tok> <cons cat="NP"><tok cat="NN">sIgM-</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">sIgD-crosslinking</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NNS">antibodies</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">elevation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cAMP</tok> <tok cat="NNS">levels</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">phosphatidylinositol</tok> <tok cat="NN">turnover</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">PKC</tok> <tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Egr-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-fos</tok></cons></cons> <tok cat="NN">mRNA</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">sIgM-crosslinking</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="RBR">more</tok> <tok cat="JJ">effective</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><tok cat="NN">sIgD-crosslinking</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">presumably</tok></cons> <tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJR">higher</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">sIgM</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="FRAG"><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">sIgD</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@19-12-2010
830229605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="NN">Egr-1</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">sIgM-</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">sIgD-crosslinking</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">H7</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">erbstatin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">genistein</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">HA1004</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
830229606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP" role="SBJ"><tok cat="NN">Erbstatin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">genistein</tok></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">sIg-crosslinking-induced</tok> <tok cat="NN">Egr-1</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons> <cons cat="ADJP" ref="i12" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">dose-dependent</tok> <tok cat="NN">manner</tok></cons></cons> <cons cat="ADJP" id="i12"><tok cat="JJ">parallel</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="WDT">that</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">sIg-crosslinking-induced</tok> <tok cat="NN">protein</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
830229607@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Phorbol</tok> <tok cat="NN">myristate</tok> <tok cat="NN">acetate</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><tok cat="NN">Egr-1</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">forskolin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">dibutyryl</tok> <tok cat="JJ">cyclic</tok> <tok cat="NN">AMP</tok></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok><cons cat="VP" null="QSTN"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
830229608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Egr-1</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">activating</tok> <tok cat="NNS">signals</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">sIgM</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">sIgD</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" syn="COOD" role="PRD"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">kinase-</tok></cons> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">PKC-dependent</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="ADJP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="JJ">A-independent</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
830229609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Cyclosporin</tok> <tok cat="NN">A</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CsA</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">FK506</tok></cons></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">rescued</tok> <cons cat="NP"><tok cat="NN">B104</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">death</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">anti-IgM</tok> <tok cat="NN">Ab</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Egr-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-fos</tok></cons></cons> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">showing</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">CsA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">FK506</tok></cons></cons> <cons cat="VP"><tok cat="VBP">affect</tok> <cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NNS">transducers</tok></cons> <cons cat="ADVP"><tok cat="RB">differently</tok></cons> <cons cat="UCP"><cons cat="UCP" syn="COOD"><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" ref="i14" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="ADVP"><tok cat="RB">downstream</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" ref="i14" null="RNR"></cons></cons></cons></cons><cons cat="NP" id="i14"><tok cat="DT">these</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@19-12-2010
830229610@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i15" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">difference</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">signals</tok></cons> <cons cat="VP"><tok cat="VBN">transduced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">sIgM</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">sIgD</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B104</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">discussed</tok><cons cat="NP" ref="i15" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
830798201@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">G(Anh)MTetra</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">natural</tok> <tok cat="JJ">bacterial</tok> <tok cat="NN">cell</tok> <tok cat="NN">wall</tok> <tok cat="NN">breakdown</tok> <tok cat="NN">product</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">interleukin-1</tok> <tok cat="NN">beta</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">interleukin-6</tok></cons></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
830798202@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">study</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">inflammatory</tok> <tok cat="NN">cytokine</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="VBN">published</tok> <tok cat="NN">erratum</tok></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNP">J</tok> <tok cat="NNP">Biol</tok> <tok cat="NNP">Chem</tok></cons> <cons cat="NP" adv="TMP"><tok cat="CD">1994</tok> <tok cat="NNP">Jun</tok> <tok cat="CD">17</tok></cons><tok cat="COLON">;</tok><cons cat="NP"><tok cat="CD">269</tok><tok cat="LRB">(</tok><tok cat="CD">24</tok><tok cat="RRB">)</tok></cons><tok cat="COLON">:</tok><cons cat="NP"><tok cat="CD">16983</tok></cons></cons></cons></cons></cons><tok cat="RRB">]</tok></cons></cons>@@@@1@30@@oe@19-12-2010
830798203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">believed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">bacterial</tok> <tok cat="NN">cell</tok> <tok cat="NN">wall</tok> <tok cat="NNS">components</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">development</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">course</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">sepsis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
830798204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i1" role="SBJ"><tok cat="JJS">most</tok> <tok cat="NN">attention</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">focused</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lipopolysaccharide</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LPS</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@19-12-2010
830798205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">studied</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok> <cons cat="S" ref="i4" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">N-acetylglucosaminyl-1,6-anhydro-N-acetylmuramyl-L-alanyl-D- isoglutamyl-m-diaminopimelyl-D-alanine</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">G(Anh)MTetra</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">naturally</tok> <tok cat="VBG">occurring</tok></cons> <tok cat="NN">breakdown</tok> <tok cat="NN">product</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">peptidoglycan</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i2"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" id="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">soluble</tok> <tok cat="JJ">lytic</tok> <tok cat="NN">transglycosylase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="FW">Escherichia</tok> <tok cat="FW">coli</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons> <cons cat="S" id="i4"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cytokine</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@19-12-2010
830798206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">G(Anh)MTetra</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP" id="i100"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="LRB">(</tok><tok cat="NN">IL</tok><tok cat="RRB">)</tok><tok cat="CD">-1</tok> <tok cat="NN">beta</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-6</tok></cons></cons> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP" id="i101" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">2</tok> <tok cat="NN">h</tok></cons> <cons cat="PP" ref="i102" null="RNR"></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><cons map="i100" cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="NN">beta</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-6</tok></cons></cons> <tok cat="NN">protein</tok> <tok cat="NN">secretion</tok></cons> <cons map="i101" cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">48</tok> <tok cat="NN">h</tok></cons> <cons cat="PP" ref="i102" null="RNR"></cons></cons></cons></cons></cons><cons cat="PP" id="i102"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
830798207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">mRNA</tok> <tok cat="NN">accumulation</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="PP" role="PRD"><cons cat="ADVP"><cons cat="ADVP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok></cons> <tok cat="RB">partly</tok></cons> <tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">rates</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">respective</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">accompanied</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">strong</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">activator</tok> <tok cat="NN">protein-1</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@19-12-2010
830798208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Experiments</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">inhibitors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">A</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">tyrosine</tok> <tok cat="JJ">kinase-dependent</tok></cons> <tok cat="NNS">pathways</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">G(Anh)MTetra-induced</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="NN">beta</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-6</tok></cons></cons> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">H7-inhibitable</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
830798209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">protein</tok> <tok cat="NN">synthesis</tok> <tok cat="NN">inhibitor</tok> <tok cat="NN">cycloheximide</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">G(Anh)MTetra-induced</tok> <tok cat="NN">IL-6</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">depends</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">synthesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">new</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">G(Anh)MTetra-induced</tok> <tok cat="NN">IL-1</tok> <tok cat="NN">beta</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">accumulation</tok></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok><cons cat="VP" null="QSTN"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
830798210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i7"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="NNS">responses</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">G(Anh)MTetra</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">LPS</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">muramyldipeptide</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MDP</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i7" null="T"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">optimal</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">G(Anh)MTetra</tok> <tok cat="NN">induction</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" syn="COOD" role="PRD"><cons cat="ADJP"><tok cat="JJ">similar</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">LPS</tok></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="ADJP"><cons cat="ADJP"><tok cat="RB">significantly</tok> <tok cat="JJR">higher</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">MDP</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@19-12-2010
830798211@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><tok cat="JJ">maximal</tok> <tok cat="JJ">G(Anh)MTetra-induced</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="NN">beta</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-6</tok></cons></cons> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">enhanced</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">co-stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">LPS</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">MDP</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="JJ">different</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">receptors</tok></cons> <tok cat="CC">and/or</tok> <cons cat="NP"><tok cat="NN">transduction</tok> <tok cat="NNS">pathways</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">involved</tok><cons cat="NP" ref="i11" null="NONE"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
830798212@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">G(Anh)MTetra</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-1</tok> <tok cat="NN">beta</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-6</tok></cons></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">possible</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">G(Anh)MTetra</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">release</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">sepsis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
831485701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">1,25-Dihydroxy</tok> <tok cat="NN">vitamin</tok> <tok cat="NN">D3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">12-O-tetradecanoyl</tok> <tok cat="NN">phorbol-13-acetate</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">synergistically</tok></cons> <cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NN">cell</tok> <tok cat="NN">differentiation</tok></cons><tok cat="COLON">:</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">FOS</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">RB</tok></cons></cons> <tok cat="NN">expression</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
831485702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">1,25-dihydroxy</tok> <tok cat="NN">vitamin</tok> <tok cat="NN">D3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">12-O-tetradecanoyl</tok> <tok cat="NN">phorbol-13-acetate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TPA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">interact</tok> <cons cat="ADVP"><tok cat="RB">synergistically</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">U937</tok> <tok cat="JJ">histiocytic</tok> <tok cat="NN">lymphoma</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
831485703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TPA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">causes</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP"><tok cat="JJ">otherwise</tok> <tok cat="JJ">ineffective</tok></cons> <tok cat="NN">dose</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">1,25-dihydroxy</tok> <tok cat="NN">vitamin</tok> <tok cat="NN">D3</tok></cons></cons></cons> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
831485704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="VBD">induced</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="VP"><tok cat="VBZ">depends</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD" adv="TMP"><cons cat="ADJP"><tok cat="JJ">simultaneous</tok></cons> <tok cat="LRB">(</tok><tok cat="CC">vs.</tok> <cons cat="ADJP"><tok cat="JJ">sequential</tok></cons><tok cat="RRB">)</tok></cons> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">agents</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
831485705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">kinetics</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">induced</tok> <tok cat="NN">differentiation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="NN">G1</tok> <tok cat="JJ">specific</tok></cons> <tok cat="JJ">cellular</tok> <tok cat="NN">response</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i9" null="ZERO"></cons><cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">initiate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">metabolic</tok> <tok cat="NN">cascade</tok></cons> <cons cat="VP"><tok cat="VBG">culminating</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
831485706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="VBD">induced</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">down-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-fos</tok> <tok cat="NN">protein</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="VBG">accompanying</tok> <tok cat="NN">up-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">RB</tok> <tok cat="NN">protein</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">possible</tok> <tok cat="NN">need</tok> <cons cat="SBAR"><tok cat="IN">for</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">up-regulated</tok> <tok cat="NN">RB</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">maintain</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">given</tok> <tok cat="VBN">differentiated</tok> <tok cat="NN">phenotype</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">suppress</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NNS">activators</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i11"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i11" id="i12" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">typically</tok></cons> <tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">proliferation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@19-12-2010
831685901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">lymphoid-specific</tok> <tok cat="NN">immunoglobulin</tok> <tok cat="NN">mu</tok> <tok cat="NN">heavy</tok> <tok cat="NN">chain</tok> <tok cat="NN">gene</tok> <tok cat="NN">enhancer</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">ETS-domain</tok> <tok cat="NNS">proteins</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@19-12-2010
831685902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">enhancer</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">immunoglobulin</tok> <tok cat="NN">mu</tok> <tok cat="NN">heavy</tok> <tok cat="NN">chain</tok> <tok cat="NN">gene</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IgH</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">heterologous</tok> <tok cat="NN">gene</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="JJ">pre-B</tok> <tok cat="NN">cell</tok></cons> <tok cat="NN">stage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
831685903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">lymphoid-specific</tok> <tok cat="NN">element</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">microB</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">necessary</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">enhancer</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">pre-B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
831685904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><tok cat="DT">A</tok> <tok cat="NN">microB</tok> <tok cat="NN">binding</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">encoded</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="NN">PU.1/Spi-1</tok> <tok cat="NN">proto-oncogene</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@19-12-2010
831685905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">Another</tok> <tok cat="NN">sequence</tok> <tok cat="NN">element</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">microA</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mu</tok> <tok cat="NN">enhancer</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">product</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ets-1</tok> <tok cat="NN">proto-oncogene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
831685906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">microA</tok> <tok cat="NN">motif</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">microB-dependent</tok> <tok cat="NN">enhancer</tok> <tok cat="NN">activity</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">a</tok> <tok cat="JJ">minimal</tok> <cons cat="ADJP"><tok cat="NN">B</tok> <tok cat="JJ">cell-specific</tok></cons> <tok cat="NN">enhancer</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">composed</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CC">both</tok> <tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PU.1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Ets-1</tok></cons></cons> <tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
831685907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Co-expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">PU.1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Ets-1</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">nonlymphoid</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="JJ">trans-activated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">reporter</tok> <tok cat="NNS">plasmids</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">minimal</tok> <tok cat="NN">mu</tok> <tok cat="NN">enhancer</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
831685908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">implicate</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Ets</tok> <tok cat="NN">family</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IgH</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
831991201@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">p65</tok> <tok cat="NN">subunit</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">regulates</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">distinct</tok> <tok cat="NNS">mechanisms</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
831991202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="NN">Transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">p50/p65</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">generally</tok></cons> <tok cat="JJ">localized</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytoplasm</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="PRPP">its</tok> <tok cat="NN">inhibitor</tok> <tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
831991203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="JJ">Overproduced</tok> <tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">free</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok></cons> <tok cat="VBN">degraded</tok><cons cat="NP" ref="i12" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
831991204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Overexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">increases</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">endogenous</tok> <tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">protein</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">carcinoma</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">lymphoid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">mechanisms</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">stabilization</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">mRNA</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
831991205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i13" role="SBJ"><cons cat="NP"><tok cat="NN">p65</tok> <tok cat="NN">delta</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">naturally</tok> <tok cat="VBG">occurring</tok></cons> <tok cat="NN">splice</tok> <tok cat="NN">variant</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">fails</tok> <cons cat="S"><cons cat="NP" ref="i13" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">markedly</tok></cons> <tok cat="VB">augment</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">protein</tok> <tok cat="NNS">levels</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
831991206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">Both</tok> <cons cat="NP"><tok cat="JJ">overexpressed</tok> <tok cat="NN">p65</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">coexpressed</tok> <tok cat="NN">p50</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">cytoplasmic</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">p65</tok> <tok cat="NN">delta</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">partly</tok> <tok cat="JJ">nuclear</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">p65</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">maintain</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytoplasm</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
831991207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i15" role="SBJ"><cons cat="NP"><tok cat="NN">p65</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">linked</tok> <cons cat="NP" ref="i15" id="i16" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">autoregulatory</tok> <tok cat="NN">loop</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" ref="i16" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">ensuring</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i17" role="SBJ"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">held</tok> <cons cat="NP" ref="i17" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytoplasm</tok></cons></cons> <cons cat="SBAR" adv="TMP"><tok cat="IN">until</tok> <cons cat="S"><cons cat="NP" id="i18" role="SBJ"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <tok cat="VBN">induced</tok> <cons cat="S"><cons cat="NP" ref="i18" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">translocate</tok> <cons cat="NP"><tok cat="PRP">it</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@19-12-2010
832120401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Cell</tok> <tok cat="NN">cycle</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">E2F</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">reveals</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">novel</tok> <tok cat="NN">E2F</tok> <tok cat="NNS">complexes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">biochemically</tok> <tok cat="JJ">distinct</tok></cons> <tok cat="NNS">forms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">free</tok> <tok cat="NN">E2F</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
832120402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">E2F</tok></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">multiple</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">proliferation</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-myc</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">dihydrofolate</tok> <tok cat="NN">reductase</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
832120403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">E2F</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NNS">interactions</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <tok cat="JJ">cellular</tok> <tok cat="NNS">proteins</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">retinoblastoma</tok> <tok cat="NN">protein</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Rb</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">Rb-related</tok> <tok cat="NN">protein</tok> <tok cat="NN">p107</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">cyclin</tok> <tok cat="NN">A</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">cdk2</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
832120404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">undertook</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">detailed</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">E2F</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NNS">activities</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok> <tok cat="NN">behavior</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
832120405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="CD">Three</tok> <tok cat="NN">E2F</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NNS">activities</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBG">resting</tok> <tok cat="LRB">(</tok><tok cat="NN">G0</tok><tok cat="RRB">)</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="PP" role="PRD"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">mobilities</tok></cons> <cons cat="ADJP"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">gel</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons></cons> <tok cat="JJ">distinct</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">previously</tok> <tok cat="VBN">defined</tok></cons> <tok cat="NN">E2F</tok> <tok cat="NNS">complexes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
832120406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="CD">One</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">activities</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">novel</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="RBR">less</tok> <tok cat="JJ">abundant</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="NN">Rb-E2F</tok></cons></cons> <tok cat="NN">complex</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
832120407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="ADJP"><tok cat="RBS">most</tok> <tok cat="JJ">prominent</tok></cons> <tok cat="NN">E2F</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBG">resting</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <tok cat="LRB">(</tok><cons cat="VP"><tok cat="VBN">termed</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><tok cat="JJ">complex</tok> <tok cat="NN">X</tok></cons></cons></cons><tok cat="RRB">)</tok></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">abundant</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">G0</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">G1</tok></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">disappeared</tok> <cons cat="SBAR" adv="TMP"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">entered</tok> <cons cat="NP"><tok cat="NN">S</tok> <tok cat="NN">phase</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">possible</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">negatively</tok> <tok cat="VBG">regulating</tok></cons> <tok cat="NN">E2F</tok> <tok cat="NN">function</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@19-12-2010
832120408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="NN">Complex</tok> <tok cat="NN">X</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">dissociated</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">adenovirus</tok> <tok cat="NN">E1A</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">requirement</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">intact</tok> <tok cat="NN">E1A</tok> <tok cat="VBN">conserved</tok> <tok cat="NN">region</tok> <tok cat="CD">2</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
832120409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i13" role="SBJ"><tok cat="NN">X</tok></cons> <cons cat="VP"><tok cat="VBD">failed</tok> <cons cat="S"><cons cat="NP" ref="i13" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">react</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">variety</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">antibodies</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Rb</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">p107</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">implicating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">E1A-binding</tok> <tok cat="NN">protein</tok></cons> <cons cat="PP"><tok cat="JJ">other</tok> <tok cat="IN">than</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Rb</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">p107</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
832120410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <tok cat="NN">addition</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="JJ">novel</tok> <tok cat="NN">E2F</tok> <tok cat="NNS">complexes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="CD">three</tok> <tok cat="JJ">distinct</tok> <tok cat="NNS">forms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><cons cat="ADJP"><tok cat="JJ">unbound</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="ADJP"><tok cat="JJ">free</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">E2F</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">resolved</tok> <cons cat="NP" ref="i14" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">gel</tok> <tok cat="NN">shift</tok> <tok cat="NNS">experiments</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
832120411@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">species</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok> <tok cat="NNS">kinetics</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@19-12-2010
832120412@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">UV</tok> <tok cat="JJ">cross-linking</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="VP"><tok cat="VBD">suggested</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i15" role="SBJ"><tok cat="DT">a</tok> <tok cat="JJ">distinct</tok> <tok cat="NN">E2F</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">uniquely</tok></cons> <tok cat="VBN">associated</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">S-phase</tok> <tok cat="NN">p107</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">Rb</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
832120413@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Together</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">E2F</tok></cons> <cons cat="VP"><tok cat="VBZ">consists</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">multiple</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="RB">biochemically</tok> <tok cat="JJ">distinct</tok></cons></cons> <tok cat="JJ">DNA-binding</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i16"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i16" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">function</tok> <cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">points</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
832595001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Single</tok> <tok cat="NN">strand</tok> <tok cat="NN">conformation</tok> <tok cat="NN">polymorphism</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">androgen</tok> <tok cat="NN">receptor</tok> <tok cat="NN">gene</tok> <tok cat="NNS">mutations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">androgen</tok> <tok cat="NN">insensitivity</tok> <tok cat="NNS">syndromes</tok></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">application</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">diagnosis</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">genetic</tok> <tok cat="NN">counseling</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">therapy</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@19-12-2010
832595002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Recent</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="NNS">mutations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">androgen</tok> <tok cat="NN">receptor</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">androgen</tok> <tok cat="NN">insensitivity</tok> <tok cat="NNS">syndromes</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">heterogeneous</tok> <tok cat="NN">group</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">related</tok> <tok cat="NNS">disorders</tok></cons> <cons cat="VP"><tok cat="VBG">involving</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">defective</tok> <tok cat="JJ">sexual</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">karyotypic</tok> <tok cat="NNS">males</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
832595003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">report</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">address</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">possibility</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">rapid</tok> <tok cat="JJ">mutational</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">androgen</tok> <tok cat="NN">receptor</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">initial</tok> <tok cat="NN">diagnosis</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">genetic</tok> <tok cat="NN">counseling</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">molecular</tok> <tok cat="NN">subclassification</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">affected</tok> <tok cat="NNS">patients</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NNS">families</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@19-12-2010
832595004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="NN">DNA</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">leukocytes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">six</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="CD">five</tok> <tok cat="NNS">families</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NNS">degrees</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">androgen</tok> <tok cat="NN">insensitivity</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">studied</tok><cons cat="NP" ref="i2" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
832595005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><tok cat="NNS">Exons</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">2</tok></cons> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="CD">8</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">androgen</tok> <tok cat="NN">receptor</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">single</tok> <tok cat="NN">strand</tok> <tok cat="NN">conformation</tok> <tok cat="NN">polymorphism</tok> <tok cat="NN">analysis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">direct</tok> <tok cat="NN">DNA</tok> <tok cat="NN">sequencing</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
832595006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="JJ">Female</tok> <tok cat="NN">family</tok> <tok cat="NNS">members</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">also</tok></cons> <tok cat="VBN">studied</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">identify</tok> <cons cat="NP"><tok cat="NN">heterozygote</tok> <tok cat="NNS">carriers</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@19-12-2010
832595007@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">Point</tok> <tok cat="NNS">mutations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">AR</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="QP"><tok cat="DT">all</tok> <tok cat="CD">six</tok></cons> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">all</tok> <tok cat="NNS">mutations</tok></cons> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><tok cat="NN">amino</tok> <tok cat="NN">acid</tok> <tok cat="NNS">substitutions</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
832595008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">One</tok> <tok cat="NN">patient</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">incomplete</tok> <tok cat="NN">androgen</tok> <tok cat="NN">insensitivity</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">mosaic</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mutation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@19-12-2010
832595009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="CD">Four</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">five</tok> <tok cat="NNS">mothers</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">young</tok> <tok cat="NN">sister</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">one</tok> <tok cat="NN">patient</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="NNS">carriers</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mutation</tok></cons> <cons cat="ADJP"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">affected</tok> <tok cat="NN">child</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
832595010@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i100" role="SBJ"><tok cat="JJ">new</tok> <tok cat="NNS">mutations</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">occur</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">androgen</tok> <tok cat="NN">receptor</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="S"><cons cat="NP" ref="i100" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">sporadic</tok> <tok cat="NN">androgen</tok> <tok cat="NN">insensitivity</tok> <tok cat="NN">syndrome</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
832595011@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Molecular</tok> <tok cat="JJ">genetic</tok> <tok cat="NN">characterization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">variant</tok> <tok cat="NN">allele</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">serve</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">primary</tok> <tok cat="NN">tool</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">diagnosis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">subsequent</tok> <tok cat="NN">therapy</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">basis</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">distinguishing</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">heterozygous</tok> <tok cat="NNS">carriers</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">familial</tok> <tok cat="NN">androgen</tok> <tok cat="NN">resistance</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
832595012@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">identification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">carriers</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="PP" role="PRD"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">substantial</tok> <tok cat="JJ">clinical</tok> <tok cat="NN">importance</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">genetic</tok> <tok cat="NN">counseling</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@19-12-2010
833089801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">mediator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">lipopolysaccharide</tok> <tok cat="NNS">effects</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@19-12-2010
833089802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Exposure</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">certain</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">bacterial</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lipopolysaccharide</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LPS</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">inducible</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
833089803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">One</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok><tok cat="POS">'s</tok></cons> <tok cat="JJ">unique</tok> <tok cat="NNS">properties</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">posttranslational</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">release</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NN">subunit</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">called</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><cons cat="NP"><cons cat="NP"><tok cat="NN">inhibitor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">sequestered</tok> <tok cat="JJ">cytoplasmic</tok> <tok cat="NN">form</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@19-12-2010
833089804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i48" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">event</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">also</tok></cons> <tok cat="VBN">triggered</tok> <cons cat="NP" ref="i48" null="NONE"></cons><cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">various</tok> <tok cat="JJ">other</tok> <tok cat="NNS">conditions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">biomedical</tok> <tok cat="NN">importance</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@19-12-2010
833089805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i49" role="SBJ"><cons cat="NP"><tok cat="JJ">Other</tok> <tok cat="JJ">bacterial</tok> <tok cat="NNS">toxins</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor-alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNF</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-1</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">mitogens</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">UV</tok> <tok cat="NN">light</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">gamma</tok> <tok cat="NNS">rays</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">oxidative</tok> <tok cat="NN">stress</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">reported</tok> <cons cat="S"><cons cat="NP" ref="i49" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@19-12-2010
833089806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">activated</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i50"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i50" id="i51" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok></cons> <tok cat="VBN">taken</tok> <cons cat="PRT"><tok cat="RP">up</tok></cons> <cons cat="NP" ref="i51" null="NONE"></cons><cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="NNS">nuclei</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">initiates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">immediate</tok> <tok cat="JJ">early</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">wide</tok> <tok cat="NN">variety</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
833089807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i52" role="SBJ"><cons cat="NP"><tok cat="JJS">Most</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">target</tok> <tok cat="NNS">genes</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="PP" role="PRD"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">relevance</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">grouped</tok> <cons cat="NP" ref="i52" null="NONE"></cons><cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="VP"><tok cat="JJ">encoding</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">cytokines</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <tok cat="NNS">receptors</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="NN">phase</tok> <tok cat="NNS">proteins</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NNS">genomes</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HIV-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@48@@oe@19-12-2010
833089808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="MD">will</tok> <cons cat="VP"><tok cat="VB">discuss</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">recent</tok> <tok cat="JJ">experimental</tok> <tok cat="NNS">evidences</tok></cons> <cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">LPS</tok></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">share</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">pathway</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">inducers</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
833089809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="JJ">common</tok> <tok cat="NN">pathway</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">involve</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">reactive</tok> <tok cat="NN">oxygen</tok> <tok cat="NNS">intermediates</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ROI</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">messenger</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
833249201@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Human</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">GATA-3</tok></cons> <cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">expression</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@19-12-2010
833249202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i17" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">family</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="VBG">activating</tok> <tok cat="NNS">proteins</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">GATA</tok> <tok cat="NNS">factors</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i17" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">consensus</tok> <tok cat="NN">motif</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">highly</tok> <tok cat="VBN">conserved</tok></cons> <tok cat="NN">C4</tok> <tok cat="NN">zinc</tok> <tok cat="NN">finger</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
833249203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i18" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="CD">One</tok> <tok cat="NN">member</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">multigene</tok> <tok cat="NN">family</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">GATA-3</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP" role="PRD"><tok cat="RBS">most</tok> <tok cat="RB">abundantly</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i18" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">target</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HIV-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">infection</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">replication</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
833249204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="ADJP"><tok cat="FW">In</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">DNase</tok> <tok cat="CD">I</tok> <tok cat="NN">footprinting</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">six</tok> <tok cat="NN">hGATA-3</tok> <tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">U3</tok> <tok cat="NN">region</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="JJ">transcriptional</tok> <tok cat="JJ">regulatory</tok></cons> <tok cat="NN">domain</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">LTR</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
833249205@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Cotransfection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">hGATA-3</tok> <tok cat="NN">expression</tok> <tok cat="NN">plasmid</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">reporter</tok> <tok cat="NN">plasmid</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i19"><tok cat="WPP">whose</tok> <tok cat="NN">transcription</tok></cons> <cons cat="S"><cons cat="NP" ref="i19" id="i20" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">directed</tok> <cons cat="NP" ref="i20" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">LTR</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">6-</tok> <tok cat="TO">to</tok> <tok cat="JJ">10-fold</tok></cons> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">LTR-mediated</tok> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">site</tok> <tok cat="JJ">specific</tok></cons> <tok cat="NN">mutation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NN">GATA</tok> <tok cat="NNS">sites</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">virtual</tok> <tok cat="NN">abrogation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">hGATA-3</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@19-12-2010
833249206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Further</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">deletion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">hGATA-3</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok> <tok cat="NN">domain</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">abolished</tok> <cons cat="NP"><tok cat="JJ">GATA-dependent</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">trans-activation</tok></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">showing</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">transcription</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" null="NONE"></cons></cons></cons></cons></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">direct</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">cotransfected</tok> <tok cat="NN">hGATA-3</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
833249207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Introduction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HIV-1</tok> <tok cat="NNS">plasmids</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i22"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i21" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">GATA</tok> <tok cat="NNS">sites</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">mutated</tok> <cons cat="NP" ref="i21" null="NONE"></cons><cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="PP" ref="i22" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons><cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">significant</tok> <tok cat="NN">reduction</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">LTR-mediated</tok> <tok cat="NN">transcription</tok></cons></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="CC">both</tok> <tok cat="DT">the</tok> <tok cat="JJ">basal</tok> <tok cat="NN">level</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PHA-</tok></cons> <tok cat="CC">plus</tok> <cons cat="NP"><tok cat="NN">PMA-</tok></cons></cons><tok cat="RRB">)</tok> <tok cat="JJ">stimulated</tok></cons> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@19-12-2010
833249208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">observations</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">addition</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">normal</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NN">gene</tok> <tok cat="NN">regulation</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">hGATA-3</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">significant</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
833591301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">preexisting</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">nuclear</tok></cons></cons> <tok cat="NNS">forms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
833591302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-AT</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="CD">3</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">inducible</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">protein</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
833591303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="NN">NF-AT</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">thought</tok> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">consist</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">components</tok></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">ubiquitous</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">inducible</tok></cons></cons> <tok cat="JJ">nuclear</tok> <tok cat="NN">component</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i3" null="T"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Fos</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Jun</tok></cons></cons> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="VBG">preexisting</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="NN">T</tok> <tok cat="JJ">cell-specific</tok></cons></cons> <tok cat="NN">component</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-ATp</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">target</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immunosuppressive</tok> <tok cat="NNS">agents</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">cyclosporin</tok> <tok cat="NN">A</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CsA</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">FK506</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@19-12-2010
833591304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">form</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">inducible</tok> <tok cat="NN">NF-AT</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">oligonucleotide</tok></cons> <cons cat="VP"><tok cat="VBG">corresponding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">distal</tok> <tok cat="NN">NF-AT</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">murine</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">hypotonic</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">unstimulated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">form</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">single</tok> <tok cat="NN">complex</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="NN">NF-ATp</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@19-12-2010
833591305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">detect</tok> <cons cat="NP"><tok cat="NN">NF-ATp</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">gel</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">purification</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">biochemical</tok> <tok cat="NNS">studies</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">strikingly</tok> <tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">precise</tok> <tok cat="NN">sequence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-AT</tok> <tok cat="NN">oligonucleotide</tok></cons> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">labeled</tok> <tok cat="NN">probe</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@19-12-2010
833591306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">present</tok> <cons cat="NP"><tok cat="NN">evidence</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">component</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">forms</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><cons cat="ADJP"><tok cat="JJ">faster-migrating</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><tok cat="LQT">"</tok><cons cat="ADJP"><tok cat="JJR">lower</tok></cons><tok cat="RQT">"</tok><tok cat="RRB">)</tok></cons></cons> <tok cat="JJ">nuclear</tok> <tok cat="NN">NF-AT</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">calcium-dependent</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">cyclosporin-sensitive</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP" adv="TMP"><tok cat="JJ">posttranslational</tok></cons></cons> <tok cat="NN">modification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-ATp</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Fos</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Jun</tok></cons></cons> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBP">stabilize</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">DNA</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@19-12-2010
833591307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">discussed</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">context</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">model</tok></cons> <cons cat="VP"><tok cat="VBG">relating</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">NF-AT</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">NF-ATp</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
834471401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Enhancing</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">17</tok> <tok cat="NN">beta-estradiol</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">NK</tok> <tok cat="NN">cell</tok> <tok cat="NN">activity</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@19-12-2010
834471402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i28" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">17</tok> <tok cat="NN">beta-estradiol</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">NK</tok> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">studied</tok><cons cat="NP" ref="i28" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@19-12-2010
834471403@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i29" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">proliferation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NK</tok> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">YT-N17</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">human</tok> <tok cat="JJ">NK-like</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">enhanced</tok> <cons cat="NP" ref="i29" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="CD">17</tok> <tok cat="NN">beta-estradiol</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">E2</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i30" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">enhancement</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i30" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="NN">tamoxifen</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Tx</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">antagonist</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">E2</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@19-12-2010
834471404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">On</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">contrary</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NN">steroid</tok> <tok cat="NNS">hormones</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Tx</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">progesterone</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">testosterone</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">effect</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
834471405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">YT-N17</tok></cons> <cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="CD">11.8</tok> <tok cat="NN">fmol/mg</tok></cons> <tok cat="NN">protein</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">estrogen</tok> <tok cat="NN">receptor</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">mean</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">independent</tok> <tok cat="NNS">assays</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">value</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i31"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i31" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="RB">5-10-fold</tok> <tok cat="JJR">higher</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="JJ">hematopoietic</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
834471406@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i32" role="SBJ"><cons cat="NP"><tok cat="DT">An</tok> <tok cat="NN">enhancement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NK</tok> <tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">E2</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">also</tok></cons> <tok cat="VBN">seen</tok> <cons cat="NP" ref="i32" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">large</tok> <tok cat="JJ">granular</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NNS">subjects</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i33" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">again</tok></cons> <tok cat="VBN">suppressed</tok> <cons cat="NP" ref="i33" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">Tx</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
834471407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">E2</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBG">activating</tok> <tok cat="NNS">factors</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">NK/LGL</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
838134901@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">antigen</tok> <tok cat="CD">2</tok></cons> <cons cat="VP"><tok cat="VBZ">interacts</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP"><tok cat="NN">EBNA2</tok> <tok cat="JJ">responsive</tok></cons> <tok cat="NN">cis-element</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">protein</tok> <tok cat="CD">1</tok> <tok cat="NN">gene</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
838134902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok> <tok cat="NN">protein</tok> <tok cat="NN">EBNA2</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">acts</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">cellular</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">viral</tok></cons></cons> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">crucial</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">immortalization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">primary</tok> <tok cat="NNS">B-cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">EBV</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
838134903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">EBNA2</tok></cons> <cons cat="VP"><tok cat="VBZ">transactivates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">promoters</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">latent</tok> <tok cat="NN">membrane</tok> <tok cat="NNS">antigens</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">LMP</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">TP1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TP2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
838134904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i60" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">promoter</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">TP1</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">chosen</tok> <cons cat="NP" ref="i60" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">model</tok> <tok cat="NN">system</tok></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i104" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">study</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">EBNA2</tok> <tok cat="JJ">mediated</tok></cons> <tok cat="NN">transactivation</tok></cons></cons></cons><cons cat="ADVP" ref="i104" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
838134905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">identify</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP"><tok cat="NN">EBNA2</tok> <tok cat="JJ">dependent</tok></cons> <tok cat="JJ">cis-acting</tok> <tok cat="NN">element</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i61" role="SBJ"><tok cat="JJ">various</tok> <tok cat="NN">TP1</tok> <tok cat="NN">promoter-reporter</tok> <tok cat="NN">gene</tok> <tok cat="NNS">constructs</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">transfected</tok> <cons cat="NP" ref="i61" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">absence</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">presence</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">EBNA2</tok> <tok cat="NN">expression</tok> <tok cat="NN">vector</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">established</tok> <tok cat="NN">B-cell</tok> <tok cat="NN">line</tok> <tok cat="NN">BL41-P3HR1</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
838134906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i62" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i62" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">delineate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="CD">81</tok> <tok cat="NN">bp</tok> <cons cat="ADJP"><tok cat="NN">EBNA2</tok> <tok cat="JJ">responsive</tok></cons> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-258</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-177</tok></cons></cons> <cons cat="PP"><tok cat="JJ">relative</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">TP1</tok> <tok cat="NN">RNA</tok> <tok cat="NN">start</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
838134907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">element</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">worked</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">either</tok> <tok cat="NN">orientation</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">mediate</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">EBNA2</tok> <tok cat="JJ">dependent</tok></cons> <tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">heterologous</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
838134908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok> <tok cat="JJ">specific</tok> <tok cat="NN">protein-DNA</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="VP"><tok cat="VBN">formed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">sequences</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="NN">EBNA2</tok> <tok cat="JJ">responsive</tok></cons> <tok cat="NN">element</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
838134909@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">Two</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">type</tok></cons> <tok cat="JJ">specific</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" id="i65" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">third</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i65" null="NONE"></cons><cons cat="PP"><tok cat="RB">only</tok> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">EBNA2</tok> <tok cat="JJ">positive</tok></cons> <tok cat="NN">cell</tok> <tok cat="NNS">extracts</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
838134910@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Gel-shift</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">presence</tok> <tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">EBNA2</tok> <tok cat="JJ">specific</tok></cons> <tok cat="JJ">monoclonal</tok> <tok cat="NNS">antibodies</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">EBNA2</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">component</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">third</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
838134911@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">EBNA2</tok></cons> <cons cat="VP"><tok cat="VBZ">interacts</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP"><tok cat="NN">EBNA2</tok> <tok cat="JJ">responsive</tok></cons> <tok cat="NN">cis-element</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">TP1</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
838332301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">interleukin</tok> <tok cat="CD">2</tok> <tok cat="JJ">CD28-responsive</tok> <tok cat="NN">complex</tok></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok> <tok cat="CD">three</tok></cons> <tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">family</tok></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Rel</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p50</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
838332302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Optimal</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP"><cons cat="QP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok> <tok cat="CD">two</tok></cons> <tok cat="NNS">signals</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@19-12-2010
838332303@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i55" role="SBJ"><tok cat="CD">One</tok> <tok cat="NN">signal</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">delivered</tok> <cons cat="NP" ref="i55" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="JJ">antigen-specific</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i56" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">second</tok> <tok cat="NN">signal</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">provided</tok> <cons cat="NP" ref="i56" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">costimulatory</tok> <tok cat="NN">molecule</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons> <cons cat="VP"><tok cat="VBN">delivered</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="JJ">antigen-presenting</tok> <tok cat="NN">cell</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
838332304@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">CD28</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">surface</tok> <tok cat="NN">molecule</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i57" role="SBJ"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" ref="i57" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">delivering</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">second</tok> <tok cat="NN">activation</tok> <tok cat="NN">signal</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
838332305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">report</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="JJ">CD28-mediated</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons> <cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">rel</tok> <tok cat="NN">family</tok> <tok cat="NNS">proteins</tok></cons><tok cat="COLON">--</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Rel</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p50</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
838332306@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">12-myristate</tok> <tok cat="NN">13-acetate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PMA</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">anti-CD28</tok> <tok cat="JJ">monoclonal</tok> <tok cat="NN">antibody</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">mAb</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">augmentation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Rel</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p50</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">augmentation</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">occur</tok> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">presence</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immunosuppressant</tok> <tok cat="NN">cyclosporin</tok> <tok cat="NN">A</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@19-12-2010
838332307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">report</tok></cons></cons> <cons cat="SBAR"><tok cat="IN">that</tok><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">PMA/anti-CD28</tok> <tok cat="NN">mAb</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">anti-CD3/anti-CD28</tok> <tok cat="NN">mAb</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i58" role="SBJ"><cons cat="NP"><tok cat="NN">c-Rel</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p50</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i58" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">CD28-responsive</tok> <tok cat="NN">element</tok></cons> <cons cat="ADJP"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">promoter</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">interleukin</tok> <tok cat="CD">2</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@19-12-2010
838332308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i59" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">functional</tok> <tok cat="NN">significance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-Rel</tok> <tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">CD28-responsive</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="NP" ref="i59" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">transfection</tok> <tok cat="NN">analysis</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHADVP" id="i60"><tok cat="WRB">where</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">cotransfection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-Rel</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">augments</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">chloramphenicol</tok> <tok cat="NN">acetyltransferase</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons> <cons cat="VP"><tok cat="VBN">linked</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">CD28-responsive</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i60" null="T"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@19-12-2010
838591601@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i74" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">lytic</tok> <tok cat="NN">transition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">imitated</tok> <cons cat="NP" ref="i74" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">recombinant</tok> <tok cat="NNS">B-cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@19-12-2010
838591602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i75" role="SBJ"><cons cat="NP"><tok cat="JJ">Lytic</tok> <tok cat="NN">transition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EBV</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">initiated</tok> <cons cat="NP" ref="i75" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">distinct</tok> <tok cat="JJ">immediate</tok> <tok cat="JJ">early</tok> <tok cat="NNS">regulators</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">viral</tok> <tok cat="NN">cycle</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP" adv="TMP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">synchronization</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP" adv="TMP"><tok cat="JJ">temporary</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">permissive</tok></cons></cons> <tok cat="NNS">conditions</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">host</tok> <tok cat="NN">cell</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
838591603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">developed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">eukaryotic</tok> <tok cat="NNS">vectors</tok></cons> <cons cat="ADJP"><tok cat="JJ">suitable</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">imitate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">processes</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">lytic</tok> <tok cat="NN">transition</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">culture</tok> <tok cat="NNS">systems</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
838591604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i77" role="SBJ"><tok cat="CD">Two</tok> <tok cat="JJ">stable</tok> <tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">established</tok><cons cat="NP" ref="i77" null="NONE"></cons></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" id="i76" role="SBJ"><tok cat="NN">R59Z</tok> <tok cat="NN">activator</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i76" id="i78" null="NONE"></cons><cons cat="S"><cons cat="NP" ref="i78" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><tok cat="JJ">lytic</tok> <tok cat="NN">EBV</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">constitutive</tok> <tok cat="NN">manner</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">BZLF</tok> <tok cat="CD">1</tok> <tok cat="NN">trans-activator</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Zta</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@34@@oe@19-12-2010
838591605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">R7-57</tok> <tok cat="NN">reporter</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">other</tok> <tok cat="NN">hand</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">signaled</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">induced</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">lytic</tok> <tok cat="NN">origin</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">EBV</tok> <tok cat="NN">replication</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ori</tok> <tok cat="NN">Lyt</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
838591606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Different</tok> <tok cat="NNS">modes</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">like</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">chemical</tok> <tok cat="NN">induction</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">lytic</tok> <tok cat="NN">superinfection</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">EBV</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">single</tok> <tok cat="NN">gene</tok> <tok cat="NN">trans-activation</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">converted</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">recombinant</tok> <tok cat="NN">ori</tok> <tok cat="NN">Lyt</tok> <tok cat="NN">element</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">R7-57</tok> <tok cat="NN">reporter</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
838591607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">BZLF</tok> <tok cat="CD">1</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">transiently</tok></cons> <tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">R7-57</tok> <tok cat="NN">reporter</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">only</tok> <tok cat="NN">EBV</tok> <tok cat="NN">trans-activator</tok></cons> <cons cat="VP"><tok cat="VBN">found</tok><cons cat="NP" null="NONE"></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">sufficient</tok> <cons cat="PP"><tok cat="NN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inducing</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">viral</tok> <tok cat="JJ">lytic</tok> <tok cat="NN">cycle</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
838591608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="VBG">Basing</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">experiments</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i79" role="SBJ"><cons cat="NP"><tok cat="JJ">trans-cellular</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">EBV</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">tested</tok> <cons cat="NP" ref="i79" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cocultivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">BZLF</tok> <tok cat="JJ">1-expressing</tok></cons> <tok cat="NN">R59Z</tok> <tok cat="NN">activator</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">R7-57</tok> <tok cat="NN">reporter</tok> <tok cat="NN">line</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
838591609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i80" role="SBJ"><cons cat="NP"><tok cat="DT">No</tok> <tok cat="JJ">lytic</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">reporter</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">measured</tok><cons cat="NP" ref="i80" null="NONE"></cons><tok cat="COMMA">,</tok> <cons cat="PP" syn="COOD"><tok cat="CC">neither</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cocultivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">activator</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <tok cat="CC">nor</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">coincubation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">BZLF</tok> <tok cat="JJ">1-containing</tok></cons> <tok cat="NN">cell</tok> <tok cat="NNS">lysates</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
838591610@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i81" role="SBJ"><tok cat="NN">Latency</tok> <tok cat="NN">breaking</tok> <tok cat="NN">activity</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">transferred</tok> <cons cat="NP" ref="i81" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">activator</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">reporter</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i82"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i83" role="SBJ"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">active</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">exogenous</tok></cons></cons> <tok cat="NN">virus</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">added</tok><cons cat="NP" ref="i83" null="NONE"></cons><cons cat="ADVP" ref="i82" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
838591611@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">cell</tok> <tok cat="NN">system</tok></cons> <cons cat="VP"><tok cat="VBN">described</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">experiments</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">provides</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">tool</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">detection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">EBV</tok> <tok cat="NN">reactivation</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">demonstrates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">potential</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">lytic</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">gene</tok> <tok cat="NN">BZLF</tok> <tok cat="CD">1</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
838693301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Hypertension</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">pregnancy</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@4@@oe@19-12-2010
838693302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Pregnancy-induced</tok> <tok cat="NN">hypertension</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PIH</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">frequent</tok> <tok cat="NN">cause</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">maternal</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">neonatal</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">morbidity</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">mortality</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
838693303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">focused</tok> <cons cat="PP"><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathophysiology</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PIH</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="RB">mainly</tok> <tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">mineralocorticoids</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="VBN">reversed</tok> <tok cat="NN">blood</tok> <tok cat="NN">pressure</tok> <tok cat="NNS">patterns</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBG">resulting</tok> <tok cat="NN">necessity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">continuous</tok> <tok cat="NN">monitoring</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">preeclamptic</tok> <tok cat="NN">mother</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@19-12-2010
838693304@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i52" role="SBJ"><cons cat="NP"><tok cat="NNS">Problems</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">antihypertensive</tok> <tok cat="NN">therapy</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">discussed</tok> <cons cat="NP" ref="i52" null="NONE"></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i53" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NNS">results</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">pilot</tok> <tok cat="NN">study</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">Urapidil</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">presented</tok><cons cat="NP" ref="i53" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
838693305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" ref="i54" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">examine</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">mineralocorticoids</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathophysiology</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PIH</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i54" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">studied</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">plasma</tok> <tok cat="NN">aldosterone</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">18-hydroxy-corticosterone</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">18-OH-B</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="NNS">levels</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">25</tok> <tok cat="NNS">women</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">PIH</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="CD">25</tok> <tok cat="JJ">healthy</tok> <tok cat="JJ">pregnant</tok> <tok cat="NNS">women</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@19-12-2010
838693306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">evaluated</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mineralocorticoid</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MR</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">count</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NNS">leukocytes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">2</tok> <tok cat="NNS">groups</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
838693307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i55" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">MR-count</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP"><tok cat="VBN">decreased</tok> <cons cat="NP" ref="i55" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">PIH-group</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@19-12-2010
838693308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">values</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">plasma</tok> <tok cat="NN">aldosterone</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">18-OH-B</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">low</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@19-12-2010
838693309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i56" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="MD">can</tok><tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">explained</tok> <cons cat="NP" ref="i56" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">receptor</tok> <tok cat="NN">down-regulation</tok></cons> <cons cat="PP"><tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJR">higher</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">mineralocorticoids</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">zona</tok> <tok cat="NN">glomerulosa</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
838693310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Perhaps</tok></cons> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">deoxycorticosterone</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="RB">hitherto</tok> <tok cat="JJ">unknown</tok> <tok cat="NN">mineralocorticoid</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">responsible</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">hypertension</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">altered</tok> <tok cat="NN">MR-status</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
838693311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i57" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">first</tok> <tok cat="NNS">results</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">continuous</tok> <tok cat="NN">blood</tok> <tok cat="NN">pressure</tok> <tok cat="NNS">measurements</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">noninvasive</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">real-time</tok></cons></cons> <tok cat="NN">blood</tok> <tok cat="NN">pressure</tok> <tok cat="NN">monitor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Finapres</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">presented</tok><cons cat="NP" ref="i57" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
838693312@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">comparison</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">obtained</tok> <tok cat="NNS">values</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">intraarterial</tok> <tok cat="NNS">measurements</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">demonstrates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">good</tok> <tok cat="NN">correlation</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="NNS">methods</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
838693313@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NNS">experiences</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">Urapidil</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">hypertension</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">severe</tok> <tok cat="NN">preeclampsia</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
838693314@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i59" role="SBJ"><cons cat="NP"><tok cat="NN">hypertension</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">preeclamptic</tok> <tok cat="NNS">women</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" ref="i59" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">Urapidil</tok></cons></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">side</tok> <tok cat="NNS">effects</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">reflex-tachycardia</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
838693315@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i60" role="SBJ"><tok cat="JJ">Further</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="MD">will</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="S"><cons cat="NP" ref="i60" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">prove</tok> <cons cat="SBAR"><tok cat="IN">if</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Urapidil</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="VBN">suited</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">prepartal</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PIH</tok></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">as</tok> <tok cat="RB">well</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
838752101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">A</tok> <tok cat="JJ">chimeric</tok> <tok cat="NN">type</tok> <tok cat="CD">II</tok><tok cat="COLON">/</tok><tok cat="NN">type</tok> <tok cat="CD">I</tok> <tok cat="NN">interleukin-1</tok> <tok cat="NN">receptor</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">mediate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-1</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
838752102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i100" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">type</tok> <tok cat="CD">I</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-1</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-1R</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S"><cons cat="NP" ref="i100" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">transducing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">signal</tok></cons> <cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
838752103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cytoplasmic</tok> <tok cat="NN">domain</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i22"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i22" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">consists</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">213</tok> <tok cat="NN">amino</tok> <tok cat="NNS">acids</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
838752104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <tok cat="NN">contrast</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">type</tok> <tok cat="CD">I</tok> <tok cat="NN">receptor</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">type</tok> <tok cat="CD">II</tok> <tok cat="NN">IL-1R</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">small</tok> <tok cat="JJ">cytoplasmic</tok> <tok cat="NN">tail</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRP">it</tok></cons> <cons cat="SBAR" ref="i23" null="EXP"></cons></cons><cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">clear</tok></cons> <cons cat="SBAR" id="i23"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">receptor</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">signal-transducing</tok> <cons cat="NP" ref="i101" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">regulatory</tok> <cons cat="NP" ref="i101" null="RNR"></cons></cons></cons><cons cat="NP" id="i101"><tok cat="NN">molecule</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@19-12-2010
838752105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">type</tok> <tok cat="CD">II</tok> <tok cat="NN">IL-1R</tok></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">mediate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
838752106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i102" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">hybrid</tok> <tok cat="NN">receptor</tok></cons> <cons cat="VP"><tok cat="VBN">composed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">extracellular</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="NN">transmembrane</tok></cons></cons> <tok cat="NNS">regions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">type</tok> <tok cat="CD">II</tok> <tok cat="NN">interleukin-1</tok></cons> <cons cat="VP"><tok cat="VBN">fused</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cytoplasmic</tok> <tok cat="NN">domain</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">type</tok> <tok cat="CD">I</tok> <tok cat="NN">IL-1R</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S"><cons cat="NP" ref="i102" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">transducing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">signal</tok></cons> <cons cat="VP" ref="i105" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">across</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">membrane</tok></cons></cons> <cons cat="VP" id="i105"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">pattern</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">activation</tok></cons> <cons cat="ADJP"><tok cat="JJ">identical</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="NN">type</tok> <tok cat="CD">I</tok> <tok cat="NN">IL-1R</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@55@@oe@19-12-2010
838752107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBD">indicated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i103" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">extracellular</tok> <tok cat="NN">domain</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">type</tok> <tok cat="CD">II</tok> <tok cat="NN">IL-1R</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S"><cons cat="NP" ref="i103" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><cons cat="ADVP"><tok cat="RB">functionally</tok></cons> <tok cat="VBG">interacting</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">interleukin-1</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBG">transmitting</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBG">resulting</tok> <tok cat="NN">signal</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">heterologous</tok> <tok cat="JJ">cytoplasmic</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
838789301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Mice</tok></cons> <cons cat="ADJP"><tok cat="NN">deficient</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="CD">55</tok> <tok cat="JJ">kd</tok></cons> <tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">resistant</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">endotoxic</tok> <tok cat="NN">shock</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="RB">yet</tok> <cons cat="VP"><tok cat="VBP">succumb</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="FW">L.</tok> <tok cat="NNS">monocytogenes</tok> <tok cat="NN">infection</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
838789302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i25" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">multiple</tok> <tok cat="JJ">biological</tok> <tok cat="NNS">activities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNF</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i25" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">distinct</tok> <tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="CD">55</tok> <tok cat="NNS">kd</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNFRp55</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">75</tok> <tok cat="NN">kd</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNFRp75</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
838789303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" ref="i26" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">targeting</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i26" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">generated</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">TNFRp55-deficient</tok> <tok cat="NN">mouse</tok> <tok cat="NN">strain</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@19-12-2010
838789304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">TNFRp55-/-mutant</tok> <tok cat="NNS">mice</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">lack</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TNFRp55</tok></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBP">display</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NNS">numbers</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NN">affinity</tok> <tok cat="NN">TNFRp75</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
838789305@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Thymocyte</tok> <tok cat="NN">development</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">lymphocyte</tok> <tok cat="NNS">populations</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unaltered</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">clonal</tok> <tok cat="NN">deletion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">potentially</tok> <tok cat="JJ">self-reactive</tok></cons> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">impaired</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
838789306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i27" role="SBJ"><tok cat="NN">TNF</tok> <tok cat="NN">signaling</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">largely</tok></cons> <tok cat="VBN">abolished</tok><cons cat="NP" ref="i27" null="NONE"></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i28" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">judged</tok> <cons cat="NP" ref="i28" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">failure</tok> <cons cat="S" ref="i100" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TNF</tok></cons></cons> <cons cat="S" id="i100"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">TNFRp55-deficient</tok> <tok cat="NNS">mice</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
838789307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">loss</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TNFRp55</tok> <tok cat="NN">function</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">renders</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">mice</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">resistant</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">lethal</tok> <tok cat="NNS">dosages</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="NNS">lipopolysaccharides</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="FW">S.</tok> <tok cat="FW">aureus</tok> <tok cat="NN">enterotoxin</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
838789308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i29" role="SBJ"><tok cat="JJ">TNFRp55-deficient</tok> <tok cat="NNS">mice</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">severely</tok> <tok cat="JJ">impaired</tok> <cons cat="S"><cons cat="NP" ref="i29" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">clear</tok> <cons cat="NP"><tok cat="FW">L.</tok> <tok cat="FW">monocytogenes</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">readily</tok></cons> <tok cat="VBP">succumb</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">infection</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
838789309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="CD">55</tok> <tok cat="NN">kd</tok> <tok cat="NN">TNFR</tok></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">decisive</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">host</tok><tok cat="POS">'s</tok></cons> <tok cat="NN">defense</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">microorganisms</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">pathogenic</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
838908501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">concatenated</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="JJ">viral</tok> <tok cat="NN">DNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">lymphoblastoid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">transfection</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">BZLF1</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
838908502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i15" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">replicative</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EBV</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">DNA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">studied</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">lymphoblastoid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">X50-7</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">6F11</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i16"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i16" id="i17" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">latently</tok></cons> <tok cat="VBN">infected</tok> <cons cat="NP" ref="i17" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
838908503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i18" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">lytic</tok> <tok cat="NN">cycle</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">EBV</tok> <tok cat="NN">infection</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i18" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">transfection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">BRLF1/BZLF1</tok> <tok cat="NN">coding</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">P3HR-1</tok> <tok cat="JJ">defective</tok> <tok cat="NN">genome</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
838908504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i19" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">combined</tok> <cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">techniques</tok></cons> <cons cat="S"><cons cat="NP" ref="i19" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">identify</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">productive</tok> <tok cat="JJ">replicative</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="JJ">viral</tok> <tok cat="NN">DNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="JJ">lytic</tok> <tok cat="JJ">cycle-induced</tok></cons> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
838908505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Restriction</tok> <tok cat="NN">enzyme</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBN">followed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">Southern</tok> <tok cat="NN">blot</tok> <tok cat="NN">hybridization</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">significant</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">fused</tok> <tok cat="NN">fragment</tok></cons> <cons cat="VP"><tok cat="VBG">encompassing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">ends</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">EBV</tok> <tok cat="NN">DNA</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
838908506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBZ">indicates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="JJ">episomal</tok> <tok cat="NN">DNA</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">concatameric</tok> <tok cat="JJ">linear</tok> <tok cat="NN">DNA</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@19-12-2010
838908507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Southern</tok> <tok cat="NN">blot</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">situ</tok></cons> <tok cat="VBG">lysing</tok> <tok cat="NNS">gels</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i64" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">content</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">linear</tok> <tok cat="NN">EBV</tok> <tok cat="NN">DNA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">also</tok></cons> <tok cat="VBN">increased</tok> <cons cat="NP" ref="i64" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">initiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">viral</tok> <tok cat="JJ">lytic</tok> <tok cat="NN">cycle</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">amount</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">circular</tok> <tok cat="NN">DNA</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">remained</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">approximately</tok> <tok cat="JJ">constant</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@19-12-2010
838908508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">propose</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">results</tok></cons></cons> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">source</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">fused</tok> <tok cat="NN">fragment</tok></cons> <cons cat="VP"><tok cat="VBG">encompassing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">ends</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">EBV</tok> <tok cat="NN">DNA</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <tok cat="VBN">concatenated</tok> <tok cat="JJ">linear</tok> <tok cat="NN">EBV</tok> <tok cat="NN">DNA</tok> <tok cat="NN">molecule</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PDT">such</tok> <tok cat="DT">a</tok> <tok cat="VBN">concatenated</tok> <tok cat="NN">molecule</tok></cons> <cons cat="ADVP"><tok cat="RBS">most</tok> <tok cat="RB">likely</tok></cons> <cons cat="VP"><tok cat="VBZ">represents</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">replicative</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">EBV</tok> <tok cat="NN">DNA</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">productively</tok> <tok cat="JJ">infected</tok></cons> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@19-12-2010
838994001@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Differential</tok> <tok cat="NN">contribution</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">herpes</tok> <tok cat="NN">simplex</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NNS">products</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok> <tok cat="NN">provirus</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
838994002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">reported</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">herpes</tok> <tok cat="NN">simplex</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HSV-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HIV-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">provirus</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
838994003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">provirus</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">correlated</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><cons cat="NP"><tok cat="CD">55-</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">85-kDa</tok></cons></cons> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">enhancer</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">50-kDa</tok> <tok cat="NN">HLP-1</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">LBP-1</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HIV-1</tok> <tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@19-12-2010
838994004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="RB">Further</tok> <tok cat="NN">examination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">system</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HSV-1</tok> <tok cat="NN">replication</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">antiviral</tok> <tok cat="NN">drug</tok> <tok cat="NN">acyclovir</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">HSV-1-mediated</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">provirus</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
838994005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Surprisingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i1" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HLP-1</tok></cons></cons> <tok cat="NN">binding</tok> <tok cat="NNS">activities</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">substantially</tok></cons> <tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">acyclovir-treated</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
838994006@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transient-transfection</tok> <tok cat="NN">assay</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">ICP0</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">ICP4</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">activated</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HIV-1</tok> <tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok> <tok cat="NN">promoter</tok> <tok cat="NN">region</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ICP0</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">greatly</tok></cons> <tok cat="VBN">enhanced</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">presence</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">binding</tok> <tok cat="NNS">proteins</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">provirus</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cooperation</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">HSV-1-activated</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">factor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">virus-encoded</tok> <tok cat="NN">transactivator</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">ICP0</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@62@@oe@19-12-2010
839209201@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">Minimally</tok> <tok cat="VBN">modified</tok> <tok cat="JJ">low</tok> <tok cat="NN">density</tok> <tok cat="JJ">lipoprotein-induced</tok></cons> <tok cat="JJ">inflammatory</tok> <tok cat="NNS">responses</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">cyclic</tok> <tok cat="NN">adenosine</tok> <tok cat="NN">monophosphate</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
839209202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">minimally</tok> <tok cat="VBN">oxidized</tok></cons> <tok cat="NN">LDL</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MM-LDL</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">activated</tok> <cons cat="NP" id="i2"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">increase</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NNS">neutrophils</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inducing</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">monocyte</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">neutrophil</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">binding</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">synthesis</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">monocyte</tok> <tok cat="JJ">chemotactic</tok> <tok cat="NN">protein-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">monocyte</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">M-CSF</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@19-12-2010
839209203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NNS">studies</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i4"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">this</tok> <tok cat="JJ">monocyte-specific</tok> <tok cat="NN">response</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">induced</tok><cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP" ref="i4" null="T"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
839209204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="CC">Both</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">monocyte</tok> <tok cat="NN">binding</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">mRNA</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">M-CSF</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">MM-LDL</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="JJ">C-depleted</tok></cons> <tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
839209205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">our</tok> <tok cat="NNS">studies</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">cAMP</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">second</tok> <tok cat="NN">messenger</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MM-LDL</tok></cons></cons> <cons cat="VP"><tok cat="VBN">cited</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">above</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
839209206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Incubation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">MM-LDL</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="CD">173</tok><tok cat="NN">%</tok></cons> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">cAMP</tok> <tok cat="NNS">levels</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
839209207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Agents</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="NP"><tok cat="NN">cAMP</tok> <tok cat="NNS">levels</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">cholera</tok> <tok cat="NN">toxin</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">pertussis</tok> <tok cat="NN">toxin</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">dibutyryl</tok> <tok cat="NN">cAMP</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">isoproterenol</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">mimicked</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">actions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MM-LDL</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
839209208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NNS">Agents</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">elevated</tok> <cons cat="NP"><tok cat="NN">cAMP</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">monocyte-endothelial</tok> <tok cat="NNS">interactions</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
839209209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">endothelial</tok> <tok cat="NN">leukocyte</tok> <tok cat="NN">adhesion</tok> <tok cat="NN">molecule</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ELAM</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">mRNA</tok> <tok cat="NN">synthesis</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i14" role="SBJ"><tok cat="NN">ELAM</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i14" null="NONE"></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i13" role="SBJ"><tok cat="NN">ELAM</tok> <tok cat="NN">mRNA</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">only</tok> <tok cat="RB">slightly</tok></cons> <cons cat="VP"><tok cat="JJ">elevated</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">MM-LDL</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@19-12-2010
839209210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">present</tok> <cons cat="NP"><tok cat="NN">evidence</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i15" role="SBJ"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">neutrophil</tok> <tok cat="NN">binding</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">LPS</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">actually</tok></cons> <cons cat="VP"><tok cat="VBN">suppressed</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">agents</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i16"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i16" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">elevated</tok> <cons cat="NP"><tok cat="NN">cAMP</tok> <tok cat="NNS">levels</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
839243701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Costimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">cAMP</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons></cons> <tok cat="NNS">pathways</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">CD3-dependent</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">persistent</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">AP-1</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
839243702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i1" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">effects</tok></cons> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">combined</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><cons cat="NP"><tok cat="NN">cAMP-</tok></cons> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok> <tok cat="LRB">(</tok><tok cat="NN">PKC</tok><tok cat="RRB">)</tok><tok cat="JJ">-dependent</tok></cons></cons> <tok cat="NNS">pathways</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="NP" ref="i1" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">different</tok> <tok cat="JJ">cellular</tok> <tok cat="NNS">systems</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">complement</tok> <cons cat="NP"><tok cat="DT">each</tok> <tok cat="JJ">other</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">activating</tok> <cons cat="NP"><tok cat="NN">cell</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">proliferation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@19-12-2010
839243703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">report</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">pathways</tok></cons></cons></cons></cons> <cons cat="NP" role="SBJ"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBD">became</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">refractory</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CD3/T</tok> <tok cat="NN">cell</tok> <tok cat="NN">receptor</tok> <tok cat="LRB">(</tok><tok cat="NN">TcR</tok><tok cat="RRB">)</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
839243704@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">preincubated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">12-myristate</tok> <tok cat="NN">13-acetate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PMA</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">dibutyryl</tok> <tok cat="NN">cAMP</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Bt2cAMP</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">displayed</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">deficient</tok> <tok cat="JJ">proliferative</tok> <tok cat="NN">ability</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">anti-CD3</tok> <tok cat="NN">mAb</tok> <tok cat="NN">stimulation</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">individually</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="NN">Bt2cAMP</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">PMA</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">responded</tok> <cons cat="ADVP"><tok cat="RB">comparably</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">untreated</tok> <tok cat="NNS">samples</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@19-12-2010
839243705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">detected</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">association</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">reduced</tok> <tok cat="JJ">mitogenic</tok> <tok cat="NN">response</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">low</tok> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-2</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">alpha</tok> <tok cat="NN">chain</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CD25</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-2R</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
839243706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">Ca2+</tok> <tok cat="NN">mobilization</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">suggested</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">CD3/TcR-dependent</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="JJ">impaired</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">PMA/Bt2cAMP-treated</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
839243707@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="ADVP"><tok cat="RB">Remarkably</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">samples</tok></cons> <cons cat="VP"><tok cat="VBD">displayed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">persistent</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">c-fos</tok> <tok cat="NN">protooncogene</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="VBN">increased</tok> <tok cat="NN">AP-1</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="NNS">variations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CREB</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">NF-kB</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">detected</tok><cons cat="NP" ref="i4" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@19-12-2010
839243708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">Neither</tok> <cons cat="NP"><tok cat="NN">Bt2cAMP</tok></cons> <tok cat="CC">nor</tok> <cons cat="NP"><tok cat="NN">PMA</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">individually</tok></cons> <cons cat="VP"><tok cat="VBD">mediated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">these</tok> <tok cat="JJ">sustained</tok> <tok cat="NNS">effects</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">therefore</tok></cons> <cons cat="VP"><tok cat="VBP">appear</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">consequence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">interplay</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="JJ">metabolic</tok> <tok cat="NNS">stimuli</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
839243709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Altogether</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">evidence</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">both</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">complement</tok> <cons cat="NP"><tok cat="DT">each</tok> <tok cat="JJ">other</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok><tok cat="COMMA">,</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">conversely</tok></cons><tok cat="COMMA">,</tok> <tok cat="VBP">downregulate</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">TcR</tok> <tok cat="NN">transduction</tok> <tok cat="NNS">mechanisms</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
839518801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">receptor</tok> <tok cat="NN">expression</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">HIV-1-infected</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@19-12-2010
839518802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP"><tok cat="NN">OBJECTIVE</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">examine</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">inter-relationship</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">infection</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="LRB">(</tok><tok cat="NN">TNF</tok><tok cat="RRB">)</tok><tok cat="NN">-alpha</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">immunoregulatory</tok> <tok cat="NN">cytokine</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i1"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i1" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">enhance</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">replication</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@32@@oe@19-12-2010
839518803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP"><tok cat="NN">DESIGN</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><tok cat="JJ">Infected</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">promyelocytic</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">promonocytic</tok></cons></cons> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP" ref="i2" null="NONE"></cons><cons cat="SBAR"><tok cat="IN">because</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="ADVP"><tok cat="RB">normally</tok></cons> <cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">types</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TNF</tok> <tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@19-12-2010
839518804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP"><tok cat="NNS">METHODS</tok><tok cat="COLON">:</tok></cons> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i3" role="SBJ"><tok cat="NN">TNF</tok> <tok cat="NN">receptor</tok> <tok cat="NN">surface</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">determined</tok> <cons cat="NP" ref="i3" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="JJ">monoclonal</tok> <tok cat="NN">antibody</tok> <tok cat="NN">recognition</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">flow</tok> <tok cat="NN">cytometry</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">gel</tok> <tok cat="NN">shift</tok> <tok cat="NN">analysis</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@19-12-2010
839518805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">HIV-1</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">quantitated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">reverse</tok> <tok cat="NN">transcriptase</tok> <tok cat="NN">assay</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@19-12-2010
839518806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP"><tok cat="NNS">RESULTS</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">OM-10.1</tok> <tok cat="JJ">promyelocytic</tok> <tok cat="NN">model</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">chronic</tok> <tok cat="NN">infection</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="JJ">TNF-alpha-induced</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">expression</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">substantial</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">75</tok> <tok cat="NN">kd</tok> <tok cat="NN">TNF</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TR75</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">expression</tok></cons></cons></cons></cons> <cons cat="SBAR"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="CD">55</tok> <tok cat="NN">kD</tok> <tok cat="NN">TNF</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TR55</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="ADVP"><tok cat="RB">dramatically</tok></cons> <cons cat="VP"><tok cat="VBN">altered</tok><cons cat="NP" ref="i6" null="NONE"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@43@@oe@19-12-2010
839518807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">series</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">uninfected</tok> <tok cat="JJ">parental</tok> <tok cat="NN">HL-60</tok> <tok cat="NNS">subclones</tok></cons></cons> <cons cat="ADVP"><tok cat="DT">all</tok></cons></cons> <cons cat="VP"><tok cat="VBD">reduced</tok> <cons cat="NP"><tok cat="NN">TR75</tok> <tok cat="NN">surface</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">treatment</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
839518808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="VBN">Enhanced</tok> <tok cat="NN">TR75</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">OM-10.1</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">followed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NN">TNF-alpha-dose</tok> <tok cat="NN">dependency</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="WDT">that</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">production</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
839518809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">An</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">TR75</tok> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">evident</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">peak</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">acute</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HL-60</tok> <tok cat="NNS">promyelocytes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
839518810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">TR55</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unaltered</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">TNF-alpha-induced</tok> <tok cat="NN">HIV</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">receptor</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">still</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">viral</tok> <tok cat="NN">activation</tok> <tok cat="NN">process</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
839518811@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Antibody</tok> <tok cat="NN">cross-linking</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TR55</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">absence</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">exogenous</tok> <tok cat="NN">TNF-alpha</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">maximal</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">expression</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">up-modulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">surface</tok> <tok cat="NN">TR75</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">OM-10.1</tok> <tok cat="NNS">cultures</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
839518812@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Surprisingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">this</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><tok cat="DT">the</tok> <tok cat="NN">case</tok></cons> <cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i9"><tok cat="RB">even</tok> <tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="DT">an</tok> <tok cat="JJ">antagonistic</tok> <tok cat="JJ">anti-TR55</tok> <tok cat="NN">antibody</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">used</tok><cons cat="NP" ref="i10" null="NONE"></cons><cons cat="ADVP" ref="i9" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
839518813@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Anti-TR55</tok> <tok cat="NN">antibody</tok> <tok cat="NN">cross-linking</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">chronically</tok> <tok cat="JJ">infected</tok></cons> <tok cat="NN">U1</tok> <tok cat="JJ">promonocytic</tok> <tok cat="NNS">cultures</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="ADVP"><tok cat="RB">only</tok> <tok cat="RB">partially</tok></cons> <cons cat="VP"><tok cat="VB">substitute</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">TNF-alpha-induced</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
839518814@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP"><tok cat="NNS">CONCLUSIONS</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="NN">HIV-1</tok> <tok cat="NN">infection</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">selectively</tok></cons> <cons cat="VP"><tok cat="VB">influence</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">surface</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TNF</tok> <tok cat="NNS">receptors</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">potentially</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBG">influencing</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">own</tok> <tok cat="NN">expression</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBG">altering</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="JJ">immunoregulatory</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@30@@oe@19-12-2010
841964401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NNS">viruses</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="JJ">heterologous</tok> <tok cat="NNS">enhancer/promoters</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="NN">replication</tok> <tok cat="JJ">competent</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">exhibit</tok> <cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NNS">tropisms</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
841964402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HIV</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">type</tok> <tok cat="CD">1</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LTR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBG">binding</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">constitutively</tok> <tok cat="VBN">expressed</tok></cons> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">Sp1</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i59"><cons cat="NP"><tok cat="DT">both</tok></cons> <cons cat="WHPP"><tok cat="IN">of</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons></cons> <cons cat="S"><cons cat="NP" ref="i59" id="i60" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">highly</tok></cons> <tok cat="VBN">conserved</tok> <cons cat="NP" ref="i60" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HIV</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">simian</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok></cons></cons> <tok cat="NNS">isolates</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@49@@oe@19-12-2010
841964403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">delineate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">motifs</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">replicative</tok> <tok cat="NN">capacity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">explore</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">possibility</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">extending</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">virus</tok> <tok cat="NN">host</tok> <tok cat="NN">range</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i61" role="SBJ"><tok cat="VBN">known</tok> <tok cat="JJ">heterologous</tok> <tok cat="NNS">enhancer/promoters</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">inserted</tok> <cons cat="NP" ref="i61" null="NONE"></cons><cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">LTR</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">place</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Sp1</tok></cons></cons> <tok cat="NN">binding</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@19-12-2010
841964404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i62" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">substitutions</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">replication</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">transfected</tok> <tok cat="NN">HeLa</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">lymphocytes</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">continuous</tok> <tok cat="NN">T-leukemia</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">evaluated</tok><cons cat="NP" ref="i62" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
841964405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i63" role="SBJ"><cons cat="NP"><tok cat="NN">HIVs</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i64"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" syn="COOD" id="i65" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-</tok><tok cat="NN">kappa</tok> <tok cat="NN">B/Sp1</tok> <tok cat="NN">enhancer</tok></cons> <tok cat="CC">plus</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">downstream</tok> <tok cat="NN">TATA</tok> <tok cat="NN">element</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">replaced</tok> <cons cat="NP" ref="i65" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">heterologous</tok> <tok cat="NNS">enhancer/promoters</tok></cons></cons> <cons cat="PP" ref="i64" null="T"></cons></cons></cons></cons></cons></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">also</tok></cons> <tok cat="VBN">constructed</tok><cons cat="NP" ref="i63" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
841964406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Viruses</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">cytomegalovirus</tok> <tok cat="JJ">immediate-early</tok> <tok cat="NN">enhancer</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">exhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">infectious</tok> <tok cat="NNS">kinetics</tok></cons> <cons cat="ADJP"><tok cat="JJ">similar</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">wild-type</tok> <tok cat="NN">HIV</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">lymphocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">AA2</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">replicated</tok> <cons cat="ADVP"><tok cat="RBR">less</tok> <tok cat="RB">efficiently</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">H9</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CEM</tok></cons></cons> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@19-12-2010
841964407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i103" role="SBJ"><tok cat="JJ">heterologous</tok> <tok cat="NN">enhancer</tok> <tok cat="NNS">elements</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S"><cons cat="NP" ref="i103" id="i104" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">restoring</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Tat</tok> <tok cat="NN">responsiveness</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HIV</tok> <tok cat="NN">LTR</tok></cons></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">context</tok> <tok cat="IN">of</tok> <cons cat="S"><cons cat="NP" ref="i104" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">directing</tok> <cons cat="NP"><tok cat="NN">reporter</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">infectious</tok> <tok cat="NN">progeny</tok> <tok cat="NNS">virions</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@19-12-2010
841993101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">mandatory</tok> <tok cat="JJ">proximal</tok> <tok cat="NN">step</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">radiation-induced</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B-</tok><tok cat="NN">lymphocyte</tok> <tok cat="NNS">precursors</tok></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">[</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="VBN">published</tok> <tok cat="NN">erratum</tok></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNP">Proc</tok> <tok cat="NNP">Natl</tok> <tok cat="NNP">Acad</tok> <tok cat="NNP">Sci</tok> <tok cat="NNP">U</tok> <tok cat="NNP">S</tok> <tok cat="NNP">A</tok></cons> <cons cat="NP" adv="TMP"><tok cat="CD">1993</tok> <tok cat="NNP">Apr</tok> <tok cat="CD">15</tok></cons><tok cat="COLON">;</tok><cons cat="NP"><tok cat="CD">90</tok><tok cat="LRB">(</tok><tok cat="CD">8</tok><tok cat="RRB">)</tok><tok cat="COLON">:</tok><tok cat="CD">3775</tok></cons></cons></cons></cons></cons><tok cat="RRB">]</tok></cons></cons></cons>@@@@1@45@@oe@19-12-2010
841993102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="VBG">Ionizing</tok> <tok cat="NN">radiation</tok></cons> <cons cat="VP"><tok cat="VBZ">triggers</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">signal</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B-lymphocyte</tok> <tok cat="NNS">precursors</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i66"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i66" id="i67" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">intimately</tok></cons> <tok cat="VBN">linked</tok> <cons cat="NP" ref="i67" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">active</tok> <tok cat="NN">protein-tyrosine</tok> <tok cat="NN">kinase</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
841993103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B-lymphocyte</tok> <tok cat="NNS">precursors</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">irradiation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NNS">gamma-rays</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">i</tok><tok cat="RRB">)</tok></cons> <cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">phosphatidylinositol</tok> <tok cat="NN">turnover</tok></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="NP"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">ii</tok><tok cat="RRB">)</tok></cons> <cons cat="NP"><tok cat="JJ">downstream</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">covalent</tok> <tok cat="NN">modification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">multiple</tok> <tok cat="JJ">serine-specific</tok> <tok cat="NN">protein</tok> <tok cat="NNS">kinases</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">iii</tok><tok cat="RRB">)</tok></cons> <cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@50@@oe@19-12-2010
841993104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i68" role="SBJ"><cons cat="NP"><tok cat="DT">All</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">radiation-induced</tok> <tok cat="NNS">signals</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">effectively</tok></cons> <tok cat="VBN">prevented</tok> <cons cat="NP" ref="i68" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="NN">protein-tyrosine</tok> <tok cat="NN">kinase</tok> <tok cat="NNS">inhibitors</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">genistein</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">herbimycin</tok> <tok cat="NN">A</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
841993105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">important</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><cons cat="ADVP"><tok cat="RB">perhaps</tok></cons> <tok cat="JJ">mandatory</tok></cons></cons> <tok cat="JJ">proximal</tok> <tok cat="NN">step</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok> <tok cat="NN">signaling</tok> <tok cat="NN">cascade</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B-lymphocyte</tok> <tok cat="NNS">precursors</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
841993106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i69" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="VBP">report</tok></cons> <cons cat="VP"><tok cat="NNS">expands</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">current</tok> <tok cat="NN">knowledge</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">radiation-induced</tok> <tok cat="NN">signaling</tok> <tok cat="NN">cascade</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i69" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">clarifying</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">chronological</tok> <tok cat="NN">sequence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">biochemical</tok> <tok cat="NNS">events</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i70"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i70" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">follow</tok> <cons cat="NP"><tok cat="NN">irradiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
842227401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">human</tok> <tok cat="JJ">putative</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NN">G0/G1</tok> <tok cat="VBP">switch</tok> <tok cat="NN">gene</tok></cons> <cons cat="ADJP"><tok cat="JJ">homologous</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">rodent</tok> <tok cat="NN">gene</tok></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">zinc-binding</tok> <tok cat="JJ">potential</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
842227402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">G0S24</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">member</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">set</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">genes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">putative</tok> <tok cat="NN">G0/G1</tok> <tok cat="NN">switch</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">genes</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i87"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i87" id="i88" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i88" null="NONE"></cons><cons cat="ADVP" adv="TMP"><tok cat="RB">transiently</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">1-2</tok></cons> <tok cat="NN">hr</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">lectin</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">cycloheximide</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">blood</tok> <tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@19-12-2010
842227403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Comparison</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">full-length</tok> <tok cat="NN">cDNA</tok> <tok cat="NN">sequence</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">corresponding</tok> <tok cat="JJ">genomic</tok> <tok cat="NN">sequence</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">reveals</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">open</tok> <tok cat="NN">reading</tok> <tok cat="NN">frame</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">326</tok> <tok cat="NN">amino</tok> <tok cat="NNS">acids</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">distributed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">across</tok> <cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">exons</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
842227404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Potential</tok> <tok cat="NN">phosphorylation</tok> <tok cat="NNS">sites</tok></cons> <cons cat="VP"><tok cat="VBP">include</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">sequence</tok> <tok cat="NN">PSPTSPT</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i89"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i89" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">resembles</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">RNA</tok> <tok cat="NN">polymerase</tok> <tok cat="CD">II</tok> <tok cat="NN">repeat</tok></cons> <cons cat="VP"><tok cat="VBN">reported</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">target</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok> <tok cat="NN">control</tok> <tok cat="NN">kinase</tok> <tok cat="NN">cdc2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
842227405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Comparison</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">derived</tok> <tok cat="NN">protein</tok> <tok cat="NN">sequence</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">rodent</tok> <tok cat="NNS">homologs</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">allows</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">classification</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="CD">three</tok> <tok cat="NNS">groups</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
842227406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Group</tok> <tok cat="CD">1</tok></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">G0S24</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">rat</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">mouse</tok></cons></cons> <tok cat="NN">TIS11</tok> <tok cat="NNS">genes</tok></cons> <tok cat="LRB">(</tok><cons cat="VP"><cons cat="ADVP"><tok cat="RB">also</tok></cons> <tok cat="VBN">known</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">TTP</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Nup475</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Zfp36</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
842227407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">group</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><tok cat="CD">three</tok> <tok cat="JJ">tetraproline</tok> <tok cat="NNS">repeats</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@19-12-2010
842227408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">Groups</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">2</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">serine-rich</tok> <tok cat="NN">region</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="LQT">"</tok><tok cat="NN">arginine</tok> <tok cat="NN">element</tok><tok cat="RQT">"</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">RRLPIF</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">carboxyl</tok> <tok cat="NN">terminus</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
842227409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">All</tok> <tok cat="NNS">groups</tok></cons> <cons cat="VP"><tok cat="VBP">contain</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><cons cat="NP"><tok cat="NN">cysteine-</tok></cons> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">histidine-rich</tok></cons></cons> <tok cat="JJ">putative</tok> <tok cat="NN">zinc</tok> <tok cat="NN">finger</tok> <tok cat="NNS">domains</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">serine-phenylalanine</tok> <tok cat="LQT">"</tok><tok cat="NNS">SFS</tok><tok cat="RQT">"</tok> <tok cat="NN">domain</tok></cons> <cons cat="ADJP"><tok cat="JJ">similar</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">part</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">large</tok> <tok cat="NN">subunit</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">eukaryotic</tok> <tok cat="NN">RNA</tok> <tok cat="NN">polymerase</tok> <tok cat="CD">II</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
842227410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Comparison</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">group</tok> <tok cat="CD">1</tok> <cons cat="UCP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">human</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">mouse</tok></cons></cons> <tok cat="JJ">genomic</tok> <tok cat="NNS">sequences</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">shows</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NN">conservation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">5'</tok> <tok cat="NN">flank</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">exons</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
842227411@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">A</tok> <tok cat="NN">CpG</tok> <tok cat="NN">island</tok></cons> <cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">germ</tok> <tok cat="NN">line</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@19-12-2010
842227412@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">G0S24</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">potential</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">5'</tok> <tok cat="NN">flank</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">intron</tok></cons></cons></cons></cons><tok cat="COLON">;</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok></cons> <cons cat="VP"><tok cat="VBP">include</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">serum</tok> <tok cat="NN">response</tok> <tok cat="NN">element</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@19-12-2010
842227413@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="UCP" syn="COOD"><cons cat="NP"><tok cat="NN">Protein</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">genomic</tok></cons></cons> <tok cat="NNS">sequences</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">similarities</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">variety</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">G0S24</tok> <tok cat="NN">product</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">similar</tok> <tok cat="NN">role</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
842245901@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Interleukin-4</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">lipopolysaccharide-induced</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-jun</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-fos</tok></cons></cons> <tok cat="NN">messenger</tok> <tok cat="NN">RNA</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">activator</tok> <tok cat="NN">protein-1</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
842245902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">studied</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-4</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-4</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lipopolysaccharide</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LPS</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">immediate</tok> <tok cat="JJ">early</tok> <tok cat="NNS">genes</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-jun</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
842245903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBP">encode</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">proteins</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i80"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i80" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">form</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">dimeric</tok> <tok cat="NN">complex</tok> <tok cat="NN">activator</tok> <tok cat="NN">protein-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AP-1</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i81"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i81" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">active</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
842245904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Maximal</tok> <tok cat="NN">accumulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-jun</tok></cons></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">messenger</tok> <tok cat="NN">RNA</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">mRNA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">occurred</tok> <cons cat="PP" adv="TMP"><cons cat="NP"><tok cat="CD">30</tok> <tok cat="NNS">minutes</tok></cons> <tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">LPS</tok> <tok cat="NN">addition</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
842245905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i82"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" id="i83" role="SBJ"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" ref="i83" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="CD">5</tok> <tok cat="NNS">hours</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">before</tok> <cons cat="NP"><tok cat="NN">LPS</tok> <tok cat="NN">activation</tok></cons></cons><cons cat="ADVP" ref="i82" null="T"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i84" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">c-fos</tok> <cons cat="NP" ref="i85" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">c-jun</tok> <cons cat="NP" ref="i85" null="RNR"></cons></cons></cons><cons cat="NP" id="i85"><tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">decreased</tok><cons cat="NP" ref="i84" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
842245906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i86" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-jun</tok></cons></cons> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">LPS-treated</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i86" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="PP" role="PRD"><tok cat="JJ">due</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJR">lower</tok> <tok cat="NN">transcription</tok> <tok cat="NN">rate</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-jun</tok></cons></cons> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
842245907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-4</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">stability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-jun</tok></cons></cons> <tok cat="NNS">transcripts</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@19-12-2010
842245908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i101" role="SBJ"><tok cat="NN">evidence</tok> <cons cat="SBAR" ref="i100" null="ICH"></cons></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" ref="i101" null="NONE"></cons><cons cat="SBAR" id="i100"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-4</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">LPS-induced</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
842245909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-4</tok></cons> <cons cat="VP"><tok cat="VBZ">suppresses</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="VBN">activated</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
842399301@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i65" role="SBJ"><cons cat="NP"><tok cat="NN">Ras</tok> <tok cat="NN">oncogene</tok> <tok cat="NN">transformation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NNS">lymphoblasts</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i65" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">lymphocyte</tok> <tok cat="NN">activation</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">block</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
842399302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">p21ras</tok> <tok cat="JJ">small</tok> <tok cat="NN">GTP</tok> <tok cat="NN">binding</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">participate</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">signal</tok> <tok cat="NN">transduction</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">neoplastic</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">transformation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">development</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">many</tok> <tok cat="JJ">different</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
842399303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">relationship</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">ras</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">transformation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
842399304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i66" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">constitutive</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">T24</tok> <tok cat="NN">Ha-ras</tok> <tok cat="NN">oncogene</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">EBV-immortalized</tok> <tok cat="NN">B</tok> <tok cat="NNS">lymphoblasts</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i66" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">interleukin</tok> <tok cat="CD">2</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha</tok> <tok cat="NN">subunit</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">impaired</tok> <tok cat="NN">immunoglobulin</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">altered</tok> <tok cat="NN">adhesion</tok> <tok cat="NNS">properties</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">survival</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">serum-free</tok> <tok cat="NN">medium</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@19-12-2010
842399305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">Since</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha</tok> <tok cat="NN">subunit</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">hallmark</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">lymphocyte</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">p21ras</tok></cons> <cons cat="ADVP"><tok cat="RB">naturally</tok></cons> <cons cat="VP"><tok cat="VBZ">triggers</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
842399306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">ras-transformed</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBD">displayed</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">fully</tok> <tok cat="JJ">functional</tok></cons> <tok cat="NN">IL-2r</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i67" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">assessed</tok> <cons cat="NP" ref="i67" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-fos</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP" adv="TMP"><tok cat="VBG">following</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="ADVP"><tok cat="RB">nevertheless</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="NN">growth</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">lymphokine</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
842399307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="VBN">decreased</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">immunoglobulin</tok> <tok cat="NNS">genes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">indicates</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i68" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">ras</tok> <tok cat="NN">oncogene</tok></cons> <cons cat="VP"><tok cat="VBZ">blocks</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">terminal</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">plasma</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">possibly</tok></cons> <tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i68" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inhibiting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">lymphocyte-specific</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
842399308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Somewhat</tok> <tok cat="RB">unexpectedly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">constitutive</tok> <tok cat="NN">p21ras</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">cause</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="VBN">increased</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">PEA1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AP1</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">PEA3</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Oct-2</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">NF-kB</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
842410101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">GATA-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NN">development</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@7@@oe@19-12-2010
842410102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">summary</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">derived</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">experimental</tok> <tok cat="NN">system</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i62"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i62" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">allows</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">us</tok></cons> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">dissect</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">red</tok> <tok cat="NN">cell</tok> <tok cat="NN">development</tok></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">genetic</tok> <tok cat="NN">level</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
842410103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">established</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">essential</tok> <tok cat="NN">nature</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADJP"><tok cat="JJ">primitive</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">definitive</tok></cons></cons> <tok cat="NN">erythropoiesis</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
842410104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="S"><cons cat="NP" ref="i63" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">ablating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">endogenous</tok> <tok cat="NN">GATA-1</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i63" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="PP" role="PRD"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">position</tok></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i64" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">introduce</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">variety</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">constructs</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i65"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i65" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">harbor</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">subtle</tok> <tok cat="NNS">modifications</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">flanking</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">protein-coding</tok></cons></cons> <tok cat="NNS">sequences</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i64" null="T"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
842410105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="VP"><cons cat="VP"><tok cat="VB">study</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">regulatory</tok> <tok cat="NNS">regions</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="NNS">domains</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">context</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">true</tok> <tok cat="JJ">erythroid</tok> <tok cat="NN">environment</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">experiments</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i66"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i66" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">have</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">possible</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">heretofore</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
842410106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">assay</tok></cons> <cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">dramatic</tok> <tok cat="NN">loss</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">red</tok> <tok cat="NN">cell</tok> <tok cat="NN">production</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRP">it</tok></cons> <cons cat="S" ref="i67" null="EXP"></cons></cons><cons cat="VP"><tok cat="MD">should</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">possible</tok></cons> <cons cat="S" id="i67"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">define</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">important</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">domains</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i68"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i68" id="i69" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">then</tok></cons> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">assayed</tok> <cons cat="NP" ref="i69" null="NONE"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RBR">less</tok> <tok cat="JJ">stringent</tok></cons> <tok cat="NNS">systems</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cell-free</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">transcription</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@19-12-2010
842410107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">ideal</tok> <tok cat="NN">situation</tok></cons> <cons cat="VP"><tok cat="MD">would</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="NNS">analyses</tok></cons> <cons cat="VP"><tok cat="VBN">conducted</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">GATA-1-</tok> <tok cat="NN">erythroid</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@19-12-2010
842410108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">impossible</tok> <cons cat="SBAR"><cons cat="WHNP" id="i70" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">generate</tok> <cons cat="NP" ref="i70" null="T"></cons></cons></cons></cons></cons></cons><cons cat="PP"><tok cat="VBN">given</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">requirement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Epo</tok> <tok cat="NN">receptor</tok> <tok cat="NN">expression</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">red</tok> <tok cat="NN">cell</tok> <tok cat="NN">viability</tok></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="FRAG"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNP">C.</tok> <tok cat="NNP">Simon</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNP">S.</tok> <tok cat="NNP">Orkin</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">unpublished</tok> <tok cat="NNS">observations</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
842410109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRP">It</tok></cons> <cons cat="S" ref="i71" null="EXP"></cons></cons><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">possible</tok></cons> <cons cat="S" id="i71"><cons cat="NP" id="i72" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">produce</tok> <cons cat="NP"><tok cat="JJ">such</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i72" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">first</tok></cons> <tok cat="VBG">expressing</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Epo</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">influence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">constitutive</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">then</tok></cons> <tok cat="VBG">targeting</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">GATA-1</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
842410110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">If</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">GATA-1-</tok> <tok cat="JJ">red</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">available</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">analyses</tok></cons> <cons cat="VP"><tok cat="MD">would</tok> <cons cat="VP"><tok cat="VB">involve</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">actual</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" ref="i73" null="RNR"></cons></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">chromatin</tok> <tok cat="NN">structure</tok></cons> <cons cat="VP"><tok cat="VBG">surrounding</tok> <cons cat="NP" ref="i73" null="RNR"></cons></cons></cons></cons><cons cat="NP" id="i73"><tok cat="DT">the</tok> <tok cat="NN">globin</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
842410111@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i74" role="SBJ"><cons cat="NP"><tok cat="JJ">Structure-function</tok> <tok cat="NNS">studies</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">GATA-1</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP" id="i75"><cons cat="ADVP"><tok cat="RB">greatly</tok></cons> <tok cat="VBN">simplified</tok> <cons cat="NP" ref="i74" null="NONE"></cons></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="S"><cons map="i74" cat="NP" id="i76" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJR">larger</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">mutants</tok></cons></cons></cons> <cons map="i75" cat="VP"><tok cat="VBN">studied</tok><cons cat="NP" ref="i76" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
842410112@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i77" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">ES</tok> <tok cat="NN">cell</tok> <tok cat="NN">system</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i77" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">alternative</tok></cons></cons> <cons cat="SBAR" adv="TMP"><tok cat="IN">until</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="VBN">targeted</tok> <tok cat="NN">erythroleukemia</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">become</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">available</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
842410113@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Other</tok> <tok cat="NNS">applications</tok></cons> <cons cat="VP"><tok cat="VBP">involve</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">introduction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="JJ">GATA-binding</tok> <tok cat="NN">protein</tok> <tok cat="NN">family</tok> <tok cat="NNS">members</tok></cons></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i78" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBP">rescue</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mutation</tok></cons></cons></cons></cons><cons cat="ADVP" ref="i78" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
842410114@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">If</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="MD">can</tok><tok cat="RB">not</tok><cons cat="VP" null="QSTN"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i79" role="SBJ"><tok cat="JJ">chimeric</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">tested</tok> <cons cat="NP" ref="i79" null="NONE"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">identify</tok> <cons cat="SBAR"><cons cat="WHNP" id="i80"><tok cat="WDT">which</tok> <tok cat="NN">amino</tok> <tok cat="NNS">acids</tok></cons> <cons cat="S"><cons cat="NP" ref="i80" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">distinguish</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">different</tok> <tok cat="NN">family</tok> <tok cat="NNS">members</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
842410115@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">feel</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">vital</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">understanding</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NN">ontogeny</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
842410116@GENIA Treebank@formal@@1@S@<cons cat="SBARQ"><cons cat="WHADVP" id="i81"><tok cat="WRB">How</tok></cons> <cons cat="SQ"><tok cat="VBZ">does</tok> <cons cat="NP" role="SBJ"><tok cat="NN">GATA-1</tok></cons> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">red</tok> <tok cat="NN">cell</tok> <tok cat="NNS">genes</tok></cons> <cons cat="PP"><tok cat="IN">like</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">globin</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Epo</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons><cons cat="ADVP" ref="i81" null="T"></cons></cons></cons><tok cat="PERIOD">?</tok></cons>@@@@1@14@@oe@19-12-2010
842410117@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" adv="TMP"><tok cat="IN">Once</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VB">identify</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">functional</tok> <tok cat="NNS">domains</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">GATA-binding</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i82" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">hope</tok> <cons cat="S"><cons cat="NP" ref="i82" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">learn</tok> <cons cat="SBAR"><cons cat="WHNP" id="i83"><tok cat="WP">what</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">GATA-1</tok></cons> <cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" ref="i83" null="T"></cons></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">basic</tok> <tok cat="NN">transcription</tok> <tok cat="NN">machinery</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">chromatin</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
842410118@GENIA Treebank@formal@@1@S@<cons cat="SQ"><tok cat="VBZ">Is</tok> <cons cat="NP" role="SBJ"><tok cat="NN">GATA-1</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">necessary</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">globin</tok> <tok cat="NN">gene</tok> <tok cat="NN">switching</tok></cons></cons></cons><tok cat="PERIOD">?</tok></cons>@@@@1@8@@oe@19-12-2010
842410119@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i84" role="SBJ"><tok cat="NN">GATA-1</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">modified</tok> <cons cat="NP" ref="i84" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">differently</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">development</tok></cons></cons> <cons cat="SBAR"><tok cat="IN">so</tok> <tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">locus</tok> <tok cat="NN">control</tok> <tok cat="NN">region</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">interact</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NN">globin</tok> <tok cat="NNS">promoters</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
842410120@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">find</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i85" role="SBJ"><cons cat="NP"><tok cat="CD">one</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i85" null="NONE"></cons><cons cat="PP" id="i86"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">embryonic</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons map="i85" cat="NP" role="SBJ"><tok cat="DT">another</tok></cons> <cons map="i86" cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="NN">globin</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
842798301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Cell-type-specific</tok> <tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="JJ">parathyroid</tok> <tok cat="JJ">hormone-related</tok></cons> <tok cat="NN">protein</tok> <tok cat="NN">gene</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">I</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HTLV-I</tok></cons><tok cat="RRB">)</tok></cons> <tok cat="NN">tax</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HTLV-II</tok> <tok cat="NN">tax</tok></cons></cons> <tok cat="NNS">proteins</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
842798302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">I</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HTLV-I</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HTLV-II</tok></cons></cons> <tok cat="NN">Tax</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">potent</tok> <tok cat="NNS">transactivators</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">viral</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">cellular</tok></cons></cons> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
842798303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><tok cat="NN">deletion</tok> <tok cat="NNS">mutants</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i40" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">downstream</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">parathyroid</tok> <tok cat="JJ">hormone-related</tok></cons> <tok cat="NN">protein</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PTHrP</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">promoter</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i40" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">responsive</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">HTLV-I</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HTLV-II</tok></cons></cons> <tok cat="NN">Tax</tok></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">AP1/c-jun</tok> <tok cat="NN">proto-oncogene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
842798304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i41" role="SBJ"><cons cat="NP"><tok cat="NN">Transactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PTHrP</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">Tax</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">seen</tok> <cons cat="NP" ref="i41" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NNS">lines</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NNS">fibroblasts</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
842798305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">A</tok> <tok cat="NN">carboxy</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">Tax</tok> <tok cat="NN">deletion</tok> <tok cat="NN">mutant</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">deficient</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CC">both</tok> <tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PTHrP</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL2R</tok> <tok cat="NN">alpha</tok></cons></cons> <tok cat="NNS">promoters</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HTLV-I</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LTR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
842798306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Exogenous</tok> <tok cat="NN">provision</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFkB</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">rescued</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL2R</tok> <tok cat="NN">alpha</tok> <tok cat="NN">expression</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">PTHrP</tok> <tok cat="NN">promoter</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
842798307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">HTLV-I</tok> <tok cat="NN">Tax</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">HTLV-II</tok> <tok cat="NN">Tax</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-jun</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">transactivate</tok> <cons cat="NP"><tok cat="NN">PTHrP</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathogenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">hypercalcemia</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">adult</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
843006901@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">c-rel</tok> <tok cat="NN">protooncogene</tok> <tok cat="NN">product</tok></cons> <cons cat="VP"><tok cat="VBZ">represses</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="JJ">p65-mediated</tok></cons> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">1</tok> <tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
843006902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LTR</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok> <tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HIV-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">5'</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">gene</tok></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">interleukin</tok> <tok cat="CD">2</tok> <tok cat="NN">receptor</tok> <tok cat="NN">alpha</tok> <tok cat="NN">subunit</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="NN">share</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">enhancer</tok> <tok cat="NNS">elements</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="JJ">inducible</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">units</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@55@@oe@19-12-2010
843006903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i56" role="SBJ"><tok cat="DT">These</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">enhancer</tok> <tok cat="NNS">elements</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">recognized</tok> <cons cat="NP" ref="i56" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">structurally</tok> <tok cat="JJ">related</tok></cons> <tok cat="NN">family</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">interactive</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i57"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i57" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">includes</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p50</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">product</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">c-rel</tok> <tok cat="NN">protooncogene</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">c-Rel</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
843006904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Recent</tok> <tok cat="JJ">biochemical</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">p65</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p50</tok></cons></cons> <cons cat="VP"><tok cat="VBP">form</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">prototypical</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">complex</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i58"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i58" id="i59" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok></cons> <tok cat="VBN">translocated</tok> <cons cat="NP" ref="i59" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytoplasm</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
843006905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="JJ">intracellular</tok> <tok cat="NN">signaling</tok> <tok cat="NN">complex</tok></cons> <cons cat="ADVP"><tok cat="RB">potently</tok></cons> <cons cat="VP"><tok cat="VBZ">stimulates</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">kappa</tok> <tok cat="JJ">B-directed</tok></cons> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HIV-1</tok> <tok cat="NN">LTR</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">strong</tok> <tok cat="NN">transactivation</tok> <tok cat="NN">domain</tok></cons> <cons cat="ADJP"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
843006906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">c-Rel</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i60"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i60" id="i61" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i61" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><tok cat="DT">either</tok> <cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">ester</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">alpha</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">delayed</tok> <tok cat="NNS">kinetics</tok></cons> <cons cat="ADJP"><tok cat="JJ">relative</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="ADVP"><tok cat="RB">markedly</tok></cons> <cons cat="VP"><tok cat="VBZ">represses</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">p65-mediated</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">units</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@19-12-2010
843006907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">These</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-Rel</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">correlate</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">activity</tok></cons></cons> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">with</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">heterodimerize</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">p50</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">c-Rel</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">competition</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">p50/p65</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">occupancy</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">enhancer</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@19-12-2010
843006908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Together</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">one</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-Rel</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="S" role="PRD"><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">physiologic</tok> <tok cat="NN">repressor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HIV-1</tok> <tok cat="NN">LTR</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok></cons></cons> <tok cat="NNS">promoters</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i64" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">serving</tok> <cons cat="S"><cons cat="NP" ref="i64" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">efficiently</tok></cons> <tok cat="VB">counter</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">strong</tok> <tok cat="JJ">transcriptional</tok> <tok cat="JJ">activating</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@19-12-2010
843723501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Replication</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">1</tok> <tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NNS">viruses</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="NN">linker</tok> <tok cat="NN">substitution</tok> <tok cat="NNS">mutations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-201</tok></cons> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="CD">-130</tok></cons></cons> <cons cat="NP"><tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
843723502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">previous</tok> <tok cat="NN">transfection</tok> <tok cat="NNS">analyses</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">chloramphenicol</tok> <tok cat="NN">acetyltransferase</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok> <tok cat="NN">system</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">determined</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">linker</tok> <tok cat="NN">substitution</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LS</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">mutations</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-201</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-130</tok></cons></cons> <tok cat="LRB">(</tok><cons cat="ADJP"><tok cat="JJ">relative</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">start</tok> <tok cat="NN">site</tok></cons></cons></cons><tok cat="RRB">)</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LTR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">moderate</tok> <tok cat="NNS">decreases</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">LTR</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">line</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="FRAG"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNP">S.L.Zeichner</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">J.Y.H.</tok> <tok cat="NNP">Kim</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNP">J.C.Alwine</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">J.Virol.</tok><tok cat="CD">65</tok><tok cat="COLON">:</tok><tok cat="CD">2436-2444</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">1991</tok></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@74@@oe@19-12-2010
843723503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">In</tok> <tok cat="NN">order</tok> <tok cat="TO">to</tok> <cons cat="S"><cons cat="NP" ref="i56" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VB">confirm</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">significance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">context</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">replication</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i56" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">constructed</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NN">LS</tok> <tok cat="NNS">mutations</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-201</tok></cons> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="CD">- 184</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-183</tok></cons> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="CD">-166</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-165</tok></cons> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="CD">-148</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-148</tok></cons> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="CD">-130</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">proviruses</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">prepared</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NNS">stocks</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cocultivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">transfected</tok> <tok cat="NN">RD</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">CEMx174</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@57@@oe@19-12-2010
843723504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i57" role="SBJ"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">mutations</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-93</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-76</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-75</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-58</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">utilized</tok><cons cat="NP" ref="i57" null="NONE"></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">since</tok> <cons cat="S"><cons cat="NP" id="i58" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">affect</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons><tok cat="RRB">)</tok></cons><tok cat="COLON">-</tok></cons> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">Sp1-binding</tok></cons></cons> <tok cat="NNS">sites</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">expected</tok> <cons cat="S"><cons cat="NP" ref="i58" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">diminish</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">replication</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@19-12-2010
843723505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="SBAR"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">transfection</tok> <tok cat="NNS">analyses</tok></cons> <cons cat="VP"><tok cat="VBP">offer</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">adequate</tok> <tok cat="NN">approximation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">LS</tok> <tok cat="NNS">mutations</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">replication</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">mutant</tok> <tok cat="JJ">viral</tok> <tok cat="NN">stock</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">presents</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RBR">more</tok> <tok cat="JJ">accurate</tok></cons> <tok cat="NN">picture</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i59"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i59" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">sometimes</tok></cons> <cons cat="PP" role="PRD"><tok cat="IN">at</tok> <tok cat="NN">variance</tok> <tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transfection</tok> <tok cat="NNS">results</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@19-12-2010
843723506@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">Three</tok> <tok cat="NNS">mutants</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-201/-184</tok> <tok cat="NN">NXS</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">-165/-148</tok> <tok cat="NN">NXS</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-147/-130</tok> <tok cat="NN">NXS</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">effects</tok></cons> <cons cat="SBAR" ref="i102" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">replication</tok></cons></cons> <cons cat="SBAR" id="i102"><cons cat="WHNP" id="i60"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i60" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><cons cat="ADVP"><tok cat="RB">much</tok> <tok cat="RBR">more</tok></cons> <tok cat="JJ">severe</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="VP"><tok cat="VBN">predicted</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">performance</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">transfection</tok> <tok cat="NNS">analyses</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i61" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NN">LS</tok> <tok cat="NNS">mutations</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-201/-184</tok> <tok cat="NN">NXS</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-183/-166</tok> <tok cat="NN">NXS</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">predicted</tok> <cons cat="NP" ref="i61" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">transfection</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@57@@oe@19-12-2010
843723507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">cell</tok> <tok cat="JJ">type-specific</tok></cons> <tok cat="NN">permissiveness</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">replication</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">some</tok> <tok cat="JJ">mutant</tok> <tok cat="NNS">viruses</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
843723508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons> <cons cat="VP"><tok cat="VBN">tested</tok><cons cat="NP" null="NONE"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">LS</tok> <tok cat="NNS">mutations</tok></cons> <cons cat="VP"><tok cat="VBD">indicated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">apparent</tok> <tok cat="NN">requirement</tok></cons> <cons cat="PP" syn="COOD"><cons cat="CONJP"><tok cat="RB">not</tok> <tok cat="RB">only</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">intact</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">SP1-binding</tok></cons></cons> <tok cat="NNS">sites</tok></cons></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">also</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NNS">regions</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">-201</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">-130</tok></cons></cons></cons> <cons cat="VP"><tok cat="RB">not</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">infectivity</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@19-12-2010
844137701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Dimerization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-KB2</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">RelA(p65)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">regulates</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">binding</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">inhibition</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B-alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MAD-3</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
844137702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i47" role="SBJ"><cons cat="NP"><tok cat="JJ">Inducible</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HIV</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i47" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
844137703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i48" role="SBJ"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">composed</tok> <cons cat="NP" ref="i48" null="NONE"></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">distinct</tok> <tok cat="NNS">subunits</tok></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" id="i49" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="CD">five</tok> <tok cat="JJ">independent</tok> <tok cat="NNS">genes</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NFKB1(p105)</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">NFKB2(p100)</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">RelA(p65)</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">c-rel</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">relB</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i50"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i50" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">encode</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">related</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i51"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i51" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">DNA</tok> <tok cat="NNS">elements</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">isolated</tok><cons cat="NP" ref="i49" null="NONE"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@19-12-2010
844137704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <tok cat="VBN">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i52" role="SBJ"><tok cat="NN">NFKB2(p49/p52)</tok></cons> <cons cat="VP"><tok cat="VBZ">acts</tok> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">concert</tok> <tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">RelA(p65)</tok></cons></cons> <cons cat="S"><cons cat="NP" ref="i52" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">stimulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HIV</tok> <tok cat="NN">enhancer</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">Jurkat</tok> <tok cat="NN">T-leukemia</tok></cons> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
844137705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">examine</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">biochemical</tok> <tok cat="NN">basis</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">NFKB2</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
844137706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" ref="i53" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><tok cat="NN">Scatchard</tok> <tok cat="NN">analysis</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i53" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">determined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">dissociation</tok> <tok cat="NNS">constants</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">homodimeric</tok> <tok cat="NN">p49</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">heterodimeric</tok> <tok cat="NN">p49/p65</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="NN">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HIV</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
844137707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">p49</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">approximately</tok> <tok cat="JJ">18-fold-lower</tok></cons> <tok cat="NN">affinity</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HIV</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">site</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">KD</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">69.1</tok> <tok cat="NN">pM</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="SBAR"><tok cat="IN">than</tok> <cons cat="SINV"><tok cat="VBZ">does</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">approximately</tok> <tok cat="JJ">50-kDa</tok></cons> <tok cat="NN">protein</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NFKB1(p50)</tok></cons></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">p105</tok></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">KD</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">3.9</tok> <tok cat="NN">pM</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons><cons cat="VP" null="QSTN"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@19-12-2010
844137708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">affinity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">heterodimeric</tok> <tok cat="NN">NFKB2(p49)/RelA(p65)</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">site</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><cons cat="ADJP"><tok cat="RB">approximately</tok> <tok cat="RB">6-fold</tok> <tok cat="JJR">higher</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">KD</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">11.8</tok> <tok cat="NN">pM</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p49</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
844137709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" ref="i106" role="SBJ" null="NONE"></cons><cons cat="ADJP" role="PRD"><tok cat="JJ">Consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">findings</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i54" role="SBJ"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">transcription</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" ref="i54" id="i106" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">18-fold</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">preformed</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">heterodimeric</tok></cons></cons> <tok cat="NN">NFKB2(p49)/RelA(p65)</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
844137710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Transcriptional</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HIV</tok> <tok cat="NN">enhancer</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">subject</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">recently</tok> <tok cat="VBN">cloned</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B-alpha</tok></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MAD-3</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
844137711@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Recombinant</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B-alpha</tok></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MAD-3</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p65</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p49/p65</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p50/p65</tok></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">stimulated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NFKB2(p49)</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">NFKB1(p50)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
844137712@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i55" role="SBJ"><cons cat="NP"><tok cat="JJ">Functional</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">HIV</tok> <tok cat="NN">reporter</tok> <tok cat="NN">plasmid</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">p49/p65</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">transiently</tok> <tok cat="VBN">transfected</tok></cons> <cons cat="ADJP"><tok cat="NN">Jurkat</tok> <tok cat="JJ">T-leukemia</tok></cons> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">also</tok></cons> <tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i55" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">coexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MAD-3</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
844137713@GENIA Treebank@formal@@1@S@<cons cat="S"><tok cat="LRB">(</tok><cons cat="NP" id="i107" role="SBJ"><tok cat="NN">ABSTRACT</tok></cons> <cons cat="VP"><tok cat="VBN">TRUNCATED</tok> <cons cat="NP" ref="i107" null="NONE"></cons><cons cat="PP"><tok cat="IN">AT</tok> <cons cat="NP"><tok cat="CD">250</tok> <tok cat="NNS">WORDS</tok></cons></cons></cons><tok cat="RRB">)</tok></cons>@@@@1@7@@oe@19-12-2010
844677401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Tat</tok> <tok cat="NN">protein</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV1</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">promonocytic</tok> <tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@19-12-2010
844677402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Numerous</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">upon</tok> <cons cat="NP"><tok cat="NN">HIV1</tok> <tok cat="NN">infection</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i51" role="SBJ"><tok cat="JJ">human</tok> <tok cat="JJ">promonocytic</tok> <tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="S"><cons cat="NP" ref="i51" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">differentiate</tok><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i52" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">indicated</tok><cons cat="NP" ref="i52" null="NONE"></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">example</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">adhesion</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
844677403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">One</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">viral</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">process</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><tok cat="DT">the</tok> <tok cat="NN">Tat</tok> <tok cat="NN">protein</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
844677404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Indeed</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i54" role="SBJ"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">viral</tok> <tok cat="NN">protein</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i53"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i53" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">productive</tok> <tok cat="NN">infection</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i54" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">display</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">growth-stimulating</tok> <tok cat="NNS">properties</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">immunomodulatory</tok> <tok cat="NNS">activities</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
844677405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">In</tok> <tok cat="NN">order</tok> <tok cat="TO">to</tok> <cons cat="S"><cons cat="NP" ref="i56" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VB">apprehend</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HIV1</tok> <tok cat="NN">tat</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inducing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">HIV1-infected</tok> <tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i55" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP"><tok cat="RB">successfully</tok></cons> <cons cat="VP"><tok cat="VBN">introduced</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">gene</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i55" id="i56" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">infecting</tok> <cons cat="NP"><tok cat="PRP">them</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">retroviral</tok> <tok cat="NNS">particles</tok> <tok cat="VBG">transducing</tok> <tok cat="NN">tat</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@19-12-2010
844677406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i57" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Tat</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">upon</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">then</tok></cons> <cons cat="VP"><tok cat="VBN">evaluated</tok> <cons cat="NP" ref="i57" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">looking</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">c-fos</tok> <cons cat="NP" ref="i101" null="RNR"></cons></cons><cons cat="SBAR" ref="i58" null="RNR"></cons></cons></cons><tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">c-fms</tok> <cons cat="NP" ref="i101" null="RNR"></cons></cons><cons cat="SBAR" ref="i58" null="RNR"></cons></cons></cons></cons><cons cat="NP" id="i101"><tok cat="NNS">proto-oncogenes</tok></cons> <cons cat="SBAR" id="i58"><cons cat="WHNP" id="i59"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i59" id="i60" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">linked</tok> <cons cat="NP" ref="i60" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">myelomonoblastic</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@19-12-2010
844677407@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Northern</tok> <tok cat="NN">blot</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="CD">two</tok> <tok cat="NNS">proto-oncogenes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i61" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">increase</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">amplified</tok> <cons cat="NP" ref="i61" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">myristate</tok> <tok cat="NN">acetate</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
844677408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i62" role="SBJ"><tok cat="DT">No</tok> <tok cat="JJ">such</tok> <tok cat="NN">increase</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i62" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">control</tok> <tok cat="NN">U937</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@19-12-2010
844677409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">among</tok> <cons cat="NP"><tok cat="NN">HIV1</tok> <tok cat="NN">gene</tok> <tok cat="NNS">products</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i63" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">Tat</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="S"><cons cat="NP" ref="i63" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">trigger</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="JJ">viral</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBZ">directs</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">progenitors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">monocyte/macrophage</tok> <tok cat="NN">lineage</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">towards</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">differentiation</tok> <tok cat="NN">stage</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i64"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NNS">antigens</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">virions</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="RBR">more</tok> <tok cat="JJ">efficient</tok></cons><cons cat="PP" ref="i64" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@48@@oe@19-12-2010
845411401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">jun-B</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="NN">target</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">autoreactive</tok> <tok cat="NNS">T-cells</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">IDDM</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@19-12-2010
845411402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Target</tok> <tok cat="NNS">antigens</tok></cons> <cons cat="VP"><tok cat="VBN">defined</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">autoantibodies</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">IDDM</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">include</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">insulin</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">putative</tok> <tok cat="NN">glycolipid</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i41"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i41" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">reacts</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">islet</tok> <tok cat="NN">cell</tok> <tok cat="NNS">antibodies</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">64,000-M(r)</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <tok cat="VBN">identified</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="JJ">glutamic</tok> <tok cat="NN">acid</tok> <tok cat="NN">decarboxylase</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
845411403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">some</tok> <tok cat="NN">IDDM</tok> <tok cat="NN">sera</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i42"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i42" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">contain</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">antibodies</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">glutamic</tok> <tok cat="NN">acid</tok> <tok cat="NN">decarboxylase</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">coprecipitate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">38,000-M(r)</tok> <tok cat="NN">protein</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NNS">islets</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
845411404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">study</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">used</tok> <cons cat="NP" id="i43"><tok cat="DT">a</tok> <tok cat="JJ">high</tok> <tok cat="NN">titer</tok> <tok cat="JJ">anti-38,000-M(r)</tok> <tok cat="NN">serum</tok></cons> <cons cat="S"><cons cat="NP" ref="i43" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">screen</tok> <cons cat="NP"><tok cat="NN">bacteriophage</tok> <tok cat="NN">lambda</tok> <tok cat="NN">cDNA</tok> <tok cat="NN">expression</tok> <tok cat="NNS">libraries</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">islet</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">placental</tok> <tok cat="NNS">clones</tok></cons></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">jun-B</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">transcription</tok> <tok cat="NN">protein</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">predicted</tok> <tok cat="CD">38,000</tok> <tok cat="NN">M(r)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@19-12-2010
845411405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">T-cells</tok></cons> <cons cat="VP"><tok cat="VBD">exhibited</tok> <cons cat="NP"><tok cat="JJ">significant</tok> <tok cat="NN">proliferation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">recombinant</tok> <tok cat="NN">fragment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">jun-B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">amino</tok> <tok cat="NNS">acids</tok> <tok cat="CD">1-180</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="QP"><cons cat="QP"><tok cat="CD">12</tok> <tok cat="IN">of</tok> <tok cat="CD">17</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">71</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="JJ">recent-onset</tok> <tok cat="NN">IDDM</tok> <tok cat="NNS">subjects</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><cons cat="QP"><tok cat="CD">8</tok> <tok cat="IN">of</tok> <tok cat="CD">16</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">50</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="JJ">ICA-positive</tok> <tok cat="JJ">first-degree</tok> <tok cat="NNS">relatives</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IDDM</tok> <tok cat="NNS">subjects</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i44"><tok cat="WP">who</tok></cons> <cons cat="S"><cons cat="NP" ref="i44" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="PP" role="PRD"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="NN">risk</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="QP"><cons cat="QP"><tok cat="CD">3</tok> <tok cat="IN">of</tok> <tok cat="CD">12</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">25</tok><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="JJ">other</tok> <tok cat="JJ">autoimmune</tok> <tok cat="NN">disease</tok> <tok cat="NNS">subjects</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="QP"><tok cat="CD">0</tok> <tok cat="IN">of</tok> <tok cat="CD">10</tok></cons> <tok cat="JJ">healthy</tok> <tok cat="NN">control</tok> <tok cat="NNS">subjects</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@69@@oe@19-12-2010
845411406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Proliferation</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">tetanus</tok> <tok cat="NN">toxoid</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">differ</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">groups</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@19-12-2010
845411407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Responses</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">jun-B</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">related</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">age</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">sex</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">leukocyte</tok> <tok cat="NN">antigen</tok> <tok cat="NN">status</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
845411408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i45" role="SBJ"><tok cat="JJ">autoreactive</tok> <tok cat="NNS">T-cells</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">identify</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="NN">antigen</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p38</tok> <tok cat="NN">jun-B</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">IDDM</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">appear</tok> <cons cat="S"><cons cat="NP" ref="i45" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">indicate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">subjects</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">risk</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">development</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">clinical</tok> <tok cat="NN">disease</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
845562701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Interaction</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B-</tok></cons> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="NN">serum</tok> <tok cat="NN">response</tok> <tok cat="JJ">factor-binding</tok></cons></cons> <tok cat="NNS">elements</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">interleukin-2</tok> <tok cat="NN">receptor</tok> <tok cat="JJ">alpha-chain</tok> <tok cat="NN">enhancer</tok></cons> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
845562702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">find</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i45" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">short</tok> <tok cat="NN">enhancer</tok> <tok cat="NN">element</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">binding</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">interleukin-2</tok> <tok cat="NN">receptor</tok> <tok cat="JJ">alpha-chain</tok> <tok cat="NN">gene</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">preferentially</tok></cons> <tok cat="VBN">activated</tok> <cons cat="NP" ref="i45" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
845562703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i46" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok> <tok cat="NN">enhancer</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="ADVP"><tok cat="RB">poorly</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">only</tok> <tok cat="RB">weakly</tok></cons> <tok cat="VBN">activated</tok> <cons cat="NP" ref="i46" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">site</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
845562704@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Serum</tok> <tok cat="NN">response</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SRF</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">site</tok></cons> <cons cat="ADJP"><tok cat="JJ">adjacent</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2R</tok> <tok cat="NN">enhancer</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">sites</tok></cons> <cons cat="ADVP"><tok cat="RB">together</tok></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><tok cat="JJ">strong</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@19-12-2010
845562705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Surprisingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">SRF</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">consistently</tok></cons> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="JJR">higher</tok></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="FRAG"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
845562706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Overexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">SRF</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">causes</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">IL-2R</tok> <tok cat="NN">enhancer</tok></cons> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">function</tok></cons></cons></cons> <cons cat="SBAR"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <cons cat="VP"><cons cat="NP" null="QSTN"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">high</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">SRF</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">functionally</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@19-12-2010
845594101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="ADJP"><tok cat="RB">transcriptionally</tok> <tok cat="JJ">active</tok></cons> <tok cat="NNS">factors</tok></cons> <cons cat="VP"><tok cat="VBG">mediating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HTLV-I</tok> <tok cat="NN">Tax</tok> <tok cat="NN">transactivator</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">enhancer</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">include</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">product</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">c-rel</tok> <tok cat="NN">proto-oncogene</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
845594102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">transactivator</tok> <tok cat="NN">HTLV-I</tok> <tok cat="NN">Tax</tok></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">promoter</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">gene</tok></cons> <cons cat="VP"><tok cat="VBG">coding</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">interleukin</tok> <tok cat="CD">2</tok> <tok cat="JJ">alpha-chain</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">site</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i38"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i38" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NN">protein</tok> <tok cat="NNS">species</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">rel</tok> <tok cat="NN">family</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@19-12-2010
845594103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Tax1</tok></cons> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">enhancer</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">motif</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="JJ">epithelial</tok> <tok cat="NN">HeLa</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">lymphoid</tok> <tok cat="NN">Jurkat</tok></cons></cons> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
845594104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i39" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i39" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">undifferentiated</tok> <tok cat="NN">embryocarcinoma</tok> <tok cat="NN">F9</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@19-12-2010
845594105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Overexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p50</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Rel</tok></cons></cons> <tok cat="NNS">proteins</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i40" role="SBJ"><cons cat="NP"><tok cat="JJ">significant</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">site</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i40" null="NONE"></cons><cons cat="PP"><tok cat="RB">only</tok> <tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Rel</tok></cons> <tok cat="CC">and</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Rel</tok></cons> <tok cat="CC">plus</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
845594106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i41" role="SBJ"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">Tax</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">12-myristate</tok> <tok cat="NN">13-acetate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PMA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i41" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">efficiently</tok></cons> <tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2R</tok> <tok cat="NN">alpha</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">site</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">PMA</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">functionally</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">inactive</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@19-12-2010
845594107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" ref="i42" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DNA</tok> <tok cat="NN">affinity</tok> <tok cat="NN">precipitation</tok> <tok cat="NN">assay</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i42" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i43" role="SBJ"><tok cat="NN">Tax1</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i43" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">efficiently</tok></cons> <tok cat="VB">induce</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Rel</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">PMA</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <tok cat="RB">not</tok><cons cat="ADJP" role="PRD" null="QSTN"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
845594108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i44" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBD">established</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">clear</tok> <tok cat="NN">difference</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">stimuli</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" ref="i44" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Rel</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">functionally</tok> <tok cat="JJ">active</tok></cons> <tok cat="NN">factor</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
845594109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">results</tok></cons></cons> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i45" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">functional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">rel</tok> <tok cat="NN">family</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i45" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">DNA</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Rel</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <tok cat="JJ">potent</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activator</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NNS">sites</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@19-12-2010
845858101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p105</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p98</tok></cons></cons> <tok cat="NN">precursor</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBP">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">active</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">NF-kappa</tok> <tok cat="JJ">B-mediated</tok></cons> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
845858102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i40" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">Rel/NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">family</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">composed</tok> <cons cat="NP" ref="i40" null="NONE"></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">distinct</tok> <tok cat="NNS">subgroups</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">proteins</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i41"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i41" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">undergo</tok> <cons cat="NP"><tok cat="JJ">proteolytic</tok> <tok cat="NN">processing</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">contain</tok> <cons cat="NP"><tok cat="NN">SWI6/ankyrin</tok> <tok cat="NNS">repeats</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">carboxyl</tok> <tok cat="NNS">termini</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p105</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p98</tok></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP"><tok cat="JJ">such</tok> <tok cat="NNS">repeats</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i42"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i42" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">do</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">require</tok> <cons cat="NP"><tok cat="NN">processing</tok></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p65</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">c-Rel</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">RelB</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Dorsal</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@54@@oe@19-12-2010
845858103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p105</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p98</tok></cons></cons> <tok cat="NNS">precursors</tok></cons> <cons cat="VP"><tok cat="VBP">share</tok> <cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="NNS">properties</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="NNS">proteins</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i43"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i43" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">contain</tok> <cons cat="NP"><tok cat="NN">SWI6/ankyrin</tok> <tok cat="NNS">repeats</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
845858104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i44" role="SBJ"><tok cat="CC">Both</tok> <cons cat="NP"><tok cat="NN">p105</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p98</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i44" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">form</tok> <cons cat="NP"><tok cat="JJ">stable</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NN">Rel/NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">family</tok> <tok cat="NNS">members</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p65</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Rel</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
845858105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Association</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">precursors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">sufficient</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cytoplasmic</tok> <tok cat="NN">retention</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">c-Rel</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i45"><cons cat="NP"><tok cat="DT">both</tok></cons> <cons cat="WHPP"><tok cat="IN">of</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons></cons> <cons cat="S"><cons cat="NP" ref="i45" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">otherwise</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">nuclear</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
845858106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i46" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="VP"><tok cat="VBP">undergo</tok> <cons cat="NP"><tok cat="JJ">stimulus-responsive</tok> <tok cat="NN">processing</tok></cons> <cons cat="S"><cons cat="NP" ref="i46" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">produce</tok> <cons cat="NP"><tok cat="JJ">active</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">p50/c-Rel</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="NN">p55/c-Rel</tok></cons></cons> <tok cat="NNS">complexes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@19-12-2010
845858107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">observations</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">second</tok> <tok cat="NN">pathway</tok></cons> <cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">induction</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHPP" id="i47"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">processing</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">precursors</tok></cons></cons></cons> <cons cat="CONJP"><tok cat="RB">rather</tok> <tok cat="IN">than</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">major</tok> <tok cat="NN">role</tok></cons><cons cat="PP" ref="i47" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
846016901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Mutual</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activator</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">inhibitor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B-alpha</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
846016902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i48" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">NK-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">complex</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">sequestered</tok> <cons cat="NP" ref="i48" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytoplasm</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NN">protein</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B-alpha</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MAD-3</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
846016903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Various</tok> <tok cat="JJ">cellular</tok> <tok cat="NNS">stimuli</tok></cons> <cons cat="VP"><tok cat="VBP">relieve</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">mechanisms</tok></cons> <cons cat="ADJP"><tok cat="RB">largely</tok> <tok cat="JJ">unknown</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">leading</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">localization</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">target</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
846016904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">stimuli</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">alpha</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">12-myristate</tok> <tok cat="NN">13-acetate</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VB">cause</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">rapid</tok> <tok cat="NN">degradation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B-alpha</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">concomitant</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i101" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">followed</tok> <cons cat="NP" ref="i101" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">dramatic</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B-alpha</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok></cons></cons> <tok cat="NN">synthesis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@53@@oe@19-12-2010
846016905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Transfection</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">reveal</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i49" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B-alpha</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">encoded</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">potently</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i49" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">homodimers</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">p65</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-Rel</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
846016906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">propose</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">model</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i50"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B-alpha</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">mutually</tok></cons> <cons cat="VP"><tok cat="VBP">regulate</tok> <cons cat="NP"><tok cat="DT">each</tok> <tok cat="JJ">other</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cycle</tok></cons></cons><cons cat="PP" ref="i50" null="T"></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" id="i52" role="SBJ"><cons cat="NP"><tok cat="JJ">saturating</tok> <tok cat="NNS">amounts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B-alpha</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">destroyed</tok> <cons cat="NP" ref="i52" null="NONE"></cons><cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><tok cat="NN">stimulation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">allowing</tok> <cons cat="NP"><cons cat="VP"><tok cat="JJ">rapid</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@41@@oe@19-12-2010
846016907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Subsequently</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i51" role="SBJ"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B-alpha</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">mRNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok></cons></cons> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">quickly</tok></cons> <tok cat="VBN">induced</tok> <cons cat="NP" ref="i51" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="VBN">activated</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
846016908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i53" role="SBJ"><cons cat="NP"><tok cat="DT">This</tok> <tok cat="NN">resurgence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B-alpha</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">acts</tok> <cons cat="S"><cons cat="NP" ref="i53" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">restore</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">equilibrium</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i54"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i55" role="SBJ"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">again</tok></cons> <cons cat="VP"><tok cat="VBN">inhibited</tok><cons cat="NP" ref="i55" null="NONE"></cons><cons cat="PP" ref="i54" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
846266201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="VBN">Induced</tok> <tok cat="JJ">myeloid</tok> <tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">K562</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">downregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">erythroid</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">megakaryocytic</tok></cons></cons> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="JJ">experimental</tok> <tok cat="NN">model</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">hemopoietic</tok> <tok cat="NN">lineage</tok> <tok cat="NN">restriction</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@19-12-2010
846266202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i28" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">human</tok> <tok cat="NN">erythroleukemia</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">K562</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="S"><cons cat="NP" ref="i28" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">differentiate</tok> <cons cat="PP"><tok cat="IN">along</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">erythroid</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">megakaryocytic</tok></cons></cons> <tok cat="NNS">lineages</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
846266203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">hexamethylene</tok> <tok cat="NN">bisacetamide</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HMBA</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">K562</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">differentiate</tok> <cons cat="PP"><tok cat="IN">along</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">third</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
846266204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i29" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">accompanied</tok> <cons cat="NP" ref="i29" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">downregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">normally</tok></cons> <tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">erythroid</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="NN">mast</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="NN">megakaryocyte</tok></cons></cons> <tok cat="NNS">lineages</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
846266205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Northern</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">coordinate</tok> <tok cat="NN">downregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">alpha</tok> <tok cat="NN">globin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">gamma</tok> <tok cat="NN">globin</tok></cons> <cons cat="CONJP"><tok cat="IN">in</tok> <tok cat="NN">addition</tok> <tok cat="TO">to</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="JJ">lineage-restricted</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">SCL</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
846266206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i30" role="SBJ"><cons cat="NP"><tok cat="NN">Proliferation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">K562</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">suppressed</tok><cons cat="NP" ref="i30" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@19-12-2010
846266207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Clonal</tok> <tok cat="NN">assay</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">suppression</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">irreversible</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">appeared</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">analogous</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">commitment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">erythroleukemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MEL</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">terminal</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
846266208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <tok cat="NN">contrast</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">MEL</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">K562</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">acquired</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">macrophage-like</tok> <tok cat="NN">morphology</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">exhibited</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">complete</tok> <tok cat="NN">failure</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">generate</tok> <cons cat="NP"><tok cat="JJ">benzidine-positive</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
846266209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Electron</tok> <tok cat="NN">microscopy</tok></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">marked</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">granules</tok></cons> <cons cat="VP"><tok cat="VBG">resembling</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="ADJP"><tok cat="JJ">specific</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NNS">eosinophils</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
846266210@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Surface</tok> <tok cat="NN">marker</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">HMBA-induced</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">expressed</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">reduced</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">glycophorin</tok> <tok cat="NN">A</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CD5</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CD7</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CD11b</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
846266211@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">No</tok> <tok cat="NN">upregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">megakaryocyte</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">lymphoid</tok></cons></cons> <tok cat="NNS">markers</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">occurred</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@19-12-2010
846266212@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">K562</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">HMBA</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">useful</tok> <tok cat="JJ">experimental</tok> <tok cat="NN">system</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">studying</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="ADJP"><tok cat="JJ">responsible</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">downmodulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">lineage-restricted</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="JJ">hemopoietic</tok> <tok cat="NN">lineage</tok> <tok cat="NN">commitment</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
846847001@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">protein</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">AP-1</tok> <tok cat="NN">family</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">component</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
846847002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Nuclear</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-AT</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activator</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
846847003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">response</tok> <tok cat="NN">element</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">sequence</tok></cons> <cons cat="VP"><tok cat="JJ">localized</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">-285/-254</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">region</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
846847004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i40" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">composition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">still</tok></cons> <cons cat="ADVP"><tok cat="RB">not</tok> <tok cat="RB">fully</tok></cons> <cons cat="VP"><tok cat="VBN">elucidated</tok><cons cat="NP" ref="i40" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@19-12-2010
846847005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">an</tok> <tok cat="NN">AP-1</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">component</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-AT</tok> <tok cat="NN">protein</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
846847006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i41" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">evidenced</tok> <cons cat="NP" ref="i41" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok> <cons cat="S" ref="i42" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">AP-1</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="S" id="i42"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">compete</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-AT</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="NN">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">capacity</tok> <cons cat="S" ref="i43" null="ICH"></cons></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">immobilized</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">anti-Jun</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">anti-Fos</tok></cons></cons> <tok cat="NNS">antibodies</tok></cons></cons> <cons cat="S" id="i43"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">deplete</tok> <cons cat="NP"><tok cat="NN">NF-AT-binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@19-12-2010
846847007@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">There</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="JJ">detectable</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="VBN">translated</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">Jun/Fos</tok> <tok cat="NN">heterodimer</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AP-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-AT</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i44" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">NF-AT</tok> <tok cat="NN">sequence</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unable</tok> <cons cat="S"><cons cat="NP" ref="i44" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Jun/Fos</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">AP-1</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@19-12-2010
846847008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">AP-1</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-AT</tok> <tok cat="NN">protein</tok> <tok cat="NN">complex</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><tok cat="JJ">NF-AT-binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="JJ">protein-protein</tok> <tok cat="NN">interaction</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
847116701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Immobilization</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">recovery</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">fusion</tok> <tok cat="NNS">proteins</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">B-lymphocyte</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="JJ">magnetic</tok> <tok cat="NN">separation</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@19-12-2010
847116702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i42" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">new</tok> <tok cat="NN">approach</tok></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i43" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">facilitate</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">immobilization</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">affinity</tok> <tok cat="NN">purification</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">recombinant</tok> <tok cat="NNS">proteins</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="VBN">selected</tok> <tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons> <cons cat="ADVP" ref="i43" null="T"></cons></cons></cons></cons></cons></cons><cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">developed</tok><cons cat="NP" ref="i42" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
847116703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">magnetic</tok> <tok cat="NNS">beads</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">attached</tok> <tok cat="NN">DNA</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="FW">Escherichia</tok> <tok cat="FW">coli</tok> <tok cat="NN">lac</tok> <tok cat="NN">operator</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i44" role="SBJ"><cons cat="NP"><tok cat="NN">fusion</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBG">comprising</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">lac</tok> <tok cat="NN">repressor</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="JJ">affinity-purified</tok> <cons cat="NP" ref="i44" null="NONE"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">recovered</tok> <cons cat="NP" ref="i44" null="NONE"></cons></cons></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">gentle</tok> <tok cat="NN">elution</tok> <tok cat="NNS">conditions</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="FRAG"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">lactose</tok> <tok cat="NN">analogue</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">enzymatic</tok> <tok cat="NNS">means</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">deoxyribonuclease</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">DNase</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">restriction</tok> <tok cat="NNS">endonucleases</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@50@@oe@19-12-2010
847116704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NN">time</tok></cons></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i45" role="SBJ"><tok cat="DT">a</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i45" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">affinity</tok> <tok cat="NN">purification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">fusion</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons> <cons cat="SBAR"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i46" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">exemplified</tok> <cons cat="NP" ref="i46" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">specific</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">gentle</tok></cons></cons> <tok cat="NN">recovery</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">beta-galactosidase</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">alkaline</tok> <tok cat="NN">phosphatase</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">bacterial</tok> <tok cat="NNS">lysates</tok></cons></cons></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="JJ">immunomagnetic</tok> <tok cat="NN">separation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@19-12-2010
847116705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i47" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">approach</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">further</tok></cons> <cons cat="VP"><tok cat="VBN">extended</tok> <cons cat="NP" ref="i47" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">separation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">efficient</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">recovery</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">elution</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">CD37</tok> <tok cat="NN">B</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
847406801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Lymphocytes</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">site</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">disease</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><cons cat="COMP" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">adenovirus</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="CD">one</tok> <tok cat="NN">cause</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">persistent</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">recurrent</tok></cons></cons> <tok cat="JJ">inflammatory</tok> <tok cat="NN">arthritis</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
847406802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i64" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">assessment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">synovial</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NN">reactivity</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">adenovirus</tok> <tok cat="NN">antigen</tok> <tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">undertaken</tok> <cons cat="NP" ref="i64" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">persistent</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">recurrent</tok></cons></cons> <tok cat="JJ">inflammatory</tok> <tok cat="NN">arthritis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
847406803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i65" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">3H-thymidine</tok> <tok cat="NN">uptake</tok> <tok cat="NN">procedure</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">employed</tok><cons cat="NP" ref="i65" null="NONE"></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">incorporating</tok> <cons cat="NP"><tok cat="JJ">multiple</tok> <tok cat="JJ">microbiological</tok> <tok cat="NNS">antigens</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@19-12-2010
847406804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i66" role="SBJ"><tok cat="CD">Five</tok> <tok cat="NNS">patients</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i66" role="SBJ" null="NONE"></cons><cons cat="PP" role="PRD"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">repeated</tok> <tok cat="JJ">maximal</tok> <tok cat="NNS">responses</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">adenovirus</tok> <tok cat="NN">antigen</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok></cons></cons></cons></cons> <cons cat="NP" role="SBJ"><tok cat="NN">adenovirus</tok> <tok cat="NN">nucleotide</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">synovial</tok> <tok cat="NN">biopsy</tok> <tok cat="NN">specimen</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
847406805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">concluded</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">adenovirus</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="CD">one</tok> <tok cat="NN">cause</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">persistent</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="JJ">recurrent</tok></cons></cons> <tok cat="JJ">inflammatory</tok> <tok cat="NN">arthritis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
849245101@GENIA Treebank@formal@@1@S@<cons cat="NP"><tok cat="LRB">[</tok><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">trend</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">molecular</tok> <tok cat="NN">biology</tok> <tok cat="NN">study</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NNS">eosinophils</tok></cons></cons></cons></cons><tok cat="RRB">]</tok></cons>@@@@1@10@@oe@19-12-2010
849245102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Recently</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="JJ">many</tok> <tok cat="NNS">investigators</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">interested</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">study</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">eosinophil</tok> <tok cat="NN">biology</tok></cons></cons></cons></cons></cons> <cons cat="SBAR"><tok cat="IN">since</tok> <cons cat="S"><cons cat="NP" id="i53" role="SBJ"><cons cat="NP"><tok cat="NNS">genes</tok> <tok cat="NN">association</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">eosinophils</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">interleukin-5</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">eosinophil</tok> <tok cat="NN">granule</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">EPO</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">ECP</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">EDN</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">MBP</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CLC</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">isolated</tok><cons cat="NP" ref="i53" null="NONE"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@19-12-2010
849245103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i54" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">basis</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">commitment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">progenitors</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">eosinophil</tok> <tok cat="NN">lineage</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">determined</tok><cons cat="NP" ref="i54" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
849245104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i55"><tok cat="IN">by</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i56" role="SBJ"><cons cat="NP"><tok cat="JJ">eosinophil-specific</tok> <tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">primary</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">secondary</tok></cons></cons> <tok cat="NN">granule</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="ADVP"><tok cat="FW">e.g.</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ECP</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">EDN</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">EPO</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">MBP</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CLC</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i56" null="NONE"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i56" null="NONE"></cons></cons></cons><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">eosinophilopoiesis</tok></cons></cons> <cons cat="PP" ref="i55" null="T"></cons></cons></cons></cons></cons></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">unknown</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@19-12-2010
849245105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">paper</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">I</tok></cons> <cons cat="VP"><tok cat="VBD">described</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">characterization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP"><tok cat="NN">eosinophil</tok> <tok cat="NN">granule</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok> <tok cat="NN">binding</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">eosinophil</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
849618801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Oxidoreductive</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@19-12-2010
849618802@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">reducing</tok> <tok cat="NN">catalyst</tok> <tok cat="NN">thioredoxin</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@19-12-2010
849618803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i14" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">oxidoreductive</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">pathway</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">pleiotropic</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok></cons></cons></cons></cons></cons> <tok cat="VBZ">has</tok> <tok cat="VBN">been</tok> <tok cat="VBN">investigated</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S"><cons cat="NP" ref="i14" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons> <cons cat="VP"><tok cat="VBN">prepared</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytosol</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">primary</tok> <tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@19-12-2010
849618804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">a</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">reducing</tok> <tok cat="NN">catalyst</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">thioredoxin</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Trx</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">major</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="JJ">B-dependent</tok></cons> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@19-12-2010
849618805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">evidence</tok></cons> <cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i15" role="SBJ"><cons cat="NP"><tok cat="NN">redox</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">Trx</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">exerted</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">step</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">dissociation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NN">molecule</tok> <tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">cytosolic-anchoring</tok> <tok cat="NN">protein</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@19-12-2010
849618806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" ref="i16" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">examine</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Trx</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">intact</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i16" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">performed</tok> <cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">assay</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="NN">chloramphenicol</tok> <tok cat="JJ">acetyltransferase-expressing</tok></cons> <tok cat="NN">plasmid</tok></cons> <cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HIV</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">effector</tok> <tok cat="NN">plasmid</tok></cons> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">Trx</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@19-12-2010
849618807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i17" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">promoter</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">greatly</tok></cons> <tok cat="VBN">augmented</tok> <cons cat="NP" ref="i17" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" role="LGS"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">co-transfecting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">Trx-expressing</tok> <tok cat="NN">plasmid</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i18"><tok cat="WPP">whose</tok> <tok cat="NN">effect</tok></cons> <cons cat="S"><cons cat="NP" ref="i18" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B-binding</tok></cons> <tok cat="NNS">sites</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
849618808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">suggested</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i20" role="SBJ"><cons cat="NP"><tok cat="NN">cysteine</tok> <tok cat="NN">residue</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i20" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">DNA-recognition</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">redox</tok> <tok cat="NN">control</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">Trx</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="JJ">B-mediated</tok></cons> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@19-12-2010
849618809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VB">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">clue</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">understanding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">process</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">AIDS</tok> <tok cat="NN">pathogenesis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">possible</tok> <tok cat="JJ">biochemical</tok> <tok cat="NN">intervention</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
850061501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">Calcium</tok> <tok cat="JJ">dependent</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-AT</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">p59fyn</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@19-12-2010
850061502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons> <cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">antigen</tok> <tok cat="NN">receptor</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i14" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">antigen</tok> <tok cat="NN">receptor</tok> <tok cat="NN">triggering</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">myristate</tok> <tok cat="NN">acetate</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i15"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i15" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">calcium</tok> <tok cat="NN">ionophore</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@19-12-2010
850061503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">Both</tok> <tok cat="DT">these</tok> <tok cat="NNS">signals</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">necessary</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@19-12-2010
850061504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i19"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" ref="i16" id="i18" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">co-transfected</tok> <cons cat="NP" ref="i18" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">construct</tok></cons> <cons cat="ADJP"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">overexpressing</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok> <tok cat="NN">p59fyn</tok></cons></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i19" null="T"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i17" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i17" id="i16" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">PMA</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
850061505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons> <cons cat="NP" role="SBJ"><tok cat="NN">p59fyn</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">replace</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">calcium</tok> <tok cat="NN">ionophore</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
850061506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i20" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p59fyn</tok></cons> <tok cat="CC">plus</tok> <cons cat="NP"><tok cat="NN">PMA</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i20" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">EGTA</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="JJ">immunosuppressant</tok> <tok cat="NN">drug</tok> <tok cat="NN">cyclosporin</tok> <tok cat="NN">A</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
850530901@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Lipopolysaccharide</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MAD3</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Rel</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">related</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <tok cat="NNS">proteins</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">monocytic</tok> <tok cat="NN">THP-1</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
850530902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i19" role="SBJ"><cons cat="NP"><tok cat="JJ">Many</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lipopolysaccharide</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LPS</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HIV</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i19" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@19-12-2010
850530903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">specific</tok> <tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B/Rel</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">family</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">LPS</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">mechanisms</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i20"><tok cat="IN">through</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i21" role="SBJ"><tok cat="JJ">LPS-generated</tok> <tok cat="NNS">signals</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">transduced</tok> <cons cat="NP" ref="i21" null="NONE"></cons><cons cat="PP" ref="i20" null="T"></cons></cons></cons></cons></cons></cons></cons><cons cat="VP"><tok cat="VBP">remain</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unclear</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
850530904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">LPS</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Rel/p50</tok> <tok cat="NNS">heterodimers</tok></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">p50/p65</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NNS">complexes</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">monocytic</tok> <tok cat="NN">THP-1</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
850530905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Nuclear</tok> <tok cat="NN">localization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">proteins</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">occurred</tok> <cons cat="PP"><tok cat="RB">concomitantly</tok> <tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">rapid</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">cytosolic</tok> <tok cat="NNS">levels</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">independent</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="NN">phorbol</tok> <tok cat="JJ">ester-sensitive</tok></cons> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
850530906@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">Within</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">24</tok> <tok cat="NN">h</tok></cons> <cons cat="PP" adv="TMP"><tok cat="VBG">following</tok> <cons cat="NP"><tok cat="NN">LPS</tok> <tok cat="NN">stimulation</tok></cons></cons></cons></cons> <cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><tok cat="DT">a</tok> <tok cat="JJ">striking</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Rel</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">p105</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p50</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cytosol</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
850530907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="VBN">increased</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">proteins</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">correlated</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">increases</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">amounts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NNS">mRNAs</tok></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">LPS</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">THP-1</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
850530908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">LPS</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">THP-1</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">MAD3</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP"><tok cat="NN">I</tok> <tok cat="NN">kappa</tok> <tok cat="JJ">B-like</tok></cons> <tok cat="NN">protein</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">rapid</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">MAD3</tok> <tok cat="NN">mRNA</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">MAD3</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="CD">2</tok> <tok cat="NN">h</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@19-12-2010
850530909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">LPS</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">monocytic</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Rel/p50</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">p50/p65</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">MAD3</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
850584701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CD8</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">antigen</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">suppressor</tok></cons></cons> <tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">CEM</tok> <tok cat="JJ">human</tok> <tok cat="JJ">leukemic</tok> <tok cat="NN">cell</tok> <tok cat="NN">clone</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
850584702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i25" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">relationship</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">effect</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <tok cat="NNS">antigens</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="NP" ref="i25" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">models</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">leukemic</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">CEM</tok> <tok cat="NN">C7</tok></cons> <cons cat="VP"><tok cat="VBN">denoted</tok> <cons cat="NP" null="NONE"></cons></cons><tok cat="LRB">(</tok><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">r+</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">ly+</tok></cons></cons><tok cat="RRB">)</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CEM</tok> <tok cat="NN">C1</tok></cons> <tok cat="LRB">(</tok><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">r+</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">ly-</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@19-12-2010
850584703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i27" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">reactivity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="JJ">monoclonal</tok> <tok cat="NNS">antibodies</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">anti-CD4-FITC</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">anti-CD8-FITC</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">anti-CD2-FITC</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">anti-calla-FITC</tok></cons></cons><tok cat="COMMA">,</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">analyzed</tok> <cons cat="NP" ref="i27" null="NONE"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="NN">flow</tok> <tok cat="NN">cytometry</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
850584704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i28" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">suppressor</tok> <tok cat="NN">function</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">determined</tok> <cons cat="NP" ref="i28" null="NONE"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">[3H]thymidine</tok> <tok cat="NN">incorporation</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="JJ">phytohemagglutinin-activated</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
850584705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Dexamethasone</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">human</tok> <tok cat="JJ">leukemic</tok> <tok cat="NN">cell</tok> <tok cat="NN">clone</tok> <tok cat="NN">CEM</tok> <tok cat="NN">C7</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">subset</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">expressing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">surface</tok> <tok cat="NN">antigen</tok> <tok cat="NN">CD8</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i29"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i29" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="UCP" syn="COOD"><cons cat="NP"><tok cat="NN">suppressor</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">cytotoxic</tok></cons></cons> <tok cat="NNS">T-lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
850584706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="NP"><tok cat="NN">comparison</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">modification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD4</tok> <tok cat="NN">antigen</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i30"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i30" id="i31" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i31" null="NONE"></cons><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">levels</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
850584707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">After</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">days</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="ADJP"><cons cat="QP"><tok cat="CD">5</tok> <tok cat="NN">x</tok> <tok cat="CD">10(-8)</tok></cons> <tok cat="NN">M</tok></cons> <tok cat="NN">dexamethasone</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">CEM</tok> <tok cat="NN">C7</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">two-fold</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">suppressor</tok> <tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="VBN">compared</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">untreated</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
850584708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">glucocorticoids</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CC">either</tok> <tok cat="DT">the</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CD8</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">CD4</tok></cons></cons> <tok cat="NNS">antigens</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">leukemic</tok> <tok cat="NN">clone</tok> <tok cat="NN">CEM</tok> <tok cat="NN">C1</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
850584709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i32" role="SBJ"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">modification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">suppressor</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">CEM</tok> <tok cat="NN">C1</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">dexamethasone</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok><cons cat="NP" ref="i32" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
850584710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="JJ">leukemic</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">studied</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">here</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><tok cat="NN">CD8</tok> <tok cat="NN">antigen</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">CD4+</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">correlates</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">lysis</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok> <tok cat="JJ">positive</tok> <tok cat="NN">cell</tok> <tok cat="NN">population</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@19-12-2010
850835801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Proliferation</tok> <tok cat="NN">index</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">prognostic</tok> <tok cat="NN">marker</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">breast</tok> <tok cat="NN">cancer</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@19-12-2010
850835802@GENIA Treebank@formal@@1@S@<cons cat="NP"><tok cat="NN">BACKGROUND</tok><tok cat="PERIOD">.</tok></cons>@@@@1@2@@oe@19-12-2010
850835803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">proliferative</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">tumors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">extensively</tok></cons> <tok cat="VBN">investigated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">approaches</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHPP" id="i7"><tok cat="IN">among</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">use</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">monoclonal</tok> <tok cat="NN">antibody</tok> <tok cat="NN">Ki-67</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">represents</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">easy</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">reliable</tok></cons></cons> <tok cat="NN">means</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">assessing</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">proliferation</tok></cons></cons></cons></cons></cons><cons cat="PP" ref="i7" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
850835804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">proliferative</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">129</tok> <tok cat="JJ">primary</tok> <tok cat="NN">breast</tok> <tok cat="NNS">cancers</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">investigated</tok><cons cat="NP" ref="i8" null="NONE"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i105" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="JJ">related</tok> <cons cat="NP" ref="i105" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">prognosis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
850835805@GENIA Treebank@formal@@1@S@<cons cat="NP"><tok cat="NNS">METHODS</tok><tok cat="PERIOD">.</tok></cons>@@@@1@2@@oe@19-12-2010
850835806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="NN">Tumor</tok> <tok cat="NNS">samples</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">129</tok> <tok cat="NNS">patients</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i11"><tok cat="WP">who</tok></cons> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">underwent</tok> <cons cat="NP"><tok cat="NN">surgery</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNP">January</tok> <tok cat="CD">1987</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNP">December</tok> <tok cat="CD">1988</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">processed</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="VBG">staining</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">immunohistochemical</tok> <tok cat="NN">procedure</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">avidin-biotin</tok> <tok cat="NN">complex</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
850835807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">median</tok> <tok cat="NN">time</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">observation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><tok cat="CD">42</tok> <tok cat="NNS">months</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">range</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="PRD"><cons cat="QP"><tok cat="CD">31-55</tok></cons> <tok cat="NNS">months</tok></cons></cons><tok cat="RRB">)</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
850835808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="JJ">Life-table</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Mantel-Cox</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i12" id="i110" null="NONE"></cons><cons cat="S"><cons cat="NP" ref="i110" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">assess</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">probability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">disease-free</tok> <tok cat="NN">survival</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">DFS</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">overall</tok> <tok cat="NN">survival</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">OS</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
850835809@GENIA Treebank@formal@@1@S@<cons cat="NP"><tok cat="NNS">RESULTS</tok><tok cat="PERIOD">.</tok></cons>@@@@1@2@@oe@19-12-2010
850835810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i14" role="SBJ"><cons cat="NP"><tok cat="NNS">Tumors</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NN">Ki-67</tok> <tok cat="NN">proliferation</tok> <tok cat="NNS">indices</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="QP"><tok cat="JJR">&gt;</tok> <tok cat="CD">20</tok></cons><tok cat="NN">%</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i14" id="i101" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJR">higher</tok> <tok cat="JJ">4-year</tok> <tok cat="NN">probability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">relapse</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">disease</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">55.3</tok><tok cat="NN">%</tok></cons> <tok cat="CC">versus</tok> <cons cat="NP"><tok cat="CD">79.1</tok><tok cat="NN">%</tok></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">0.003</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">death</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">71</tok><tok cat="NN">%</tok></cons> <tok cat="CC">versus</tok> <cons cat="NP"><tok cat="CD">95.6</tok><tok cat="NN">%</tok></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">P</tok></cons> <cons cat="VP"><tok cat="JJ">=</tok> <cons cat="NP" role="PRD"><tok cat="CD">0.00005</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i15"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" ref="i101" id="i102" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" ref="i102" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">tumors</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">low</tok> <tok cat="NN">Ki-67</tok> <tok cat="NNS">values</tok></cons></cons></cons></cons><cons cat="ADVP" ref="i15" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@55@@oe@19-12-2010
850835811@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="JJ">proliferative</tok> <tok cat="NN">parameter</tok></cons> <cons cat="VP"><tok cat="VBD">maintained</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">prognostic</tok> <tok cat="NN">significance</tok></cons> <cons cat="SBAR" adv="TMP"><cons cat="WHADVP" id="i16"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i17" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">patients</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">stratified</tok> <cons cat="NP" ref="i17" null="NONE"></cons><cons cat="PP"><tok cat="VBG">according</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">lymph</tok> <tok cat="NN">node</tok> <tok cat="NN">involvement</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">menopausal</tok> <tok cat="NN">status</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">estrogen</tok> <tok cat="NN">receptor</tok> <tok cat="NN">content</tok></cons></cons></cons></cons><cons cat="ADVP" ref="i16" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
850835812@GENIA Treebank@formal@@1@S@<cons cat="NP"><tok cat="NNS">CONCLUSIONS</tok><tok cat="PERIOD">.</tok></cons>@@@@1@2@@oe@19-12-2010
850835813@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i18" role="SBJ"><cons cat="NP"><tok cat="NN">Tumor</tok> <tok cat="JJ">proliferative</tok> <tok cat="NN">activity</tok></cons> <cons cat="SBAR"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i19" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">evaluated</tok> <cons cat="NP" ref="i19" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">monoclonal</tok> <tok cat="NN">antibody</tok> <tok cat="NN">Ki-67</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">seems</tok> <cons cat="S"><cons cat="NP" ref="i18" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">effective</tok> <tok cat="NN">indicator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">prognosis</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">breast</tok> <tok cat="NN">cancer</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">DFS</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">OS</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
851151901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">sepsis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@19-12-2010
851151902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i69" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Glucocorticoid</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GR</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">hormone-binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">studied</tok> <cons cat="NP" ref="i69" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">whole-cell</tok> <tok cat="NN">method</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MNC</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">7</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">hemodynamic</tok> <tok cat="JJ">compensatory</tok> <tok cat="NN">phase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">sepsis</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
851151903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="CD">4</tok> <tok cat="NNS">patients</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBG">receiving</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">dopamine</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i70"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i70" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">GR</tok> <tok cat="NN">count</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
851151904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">patients</tok><tok cat="POS">'</tok></cons> <tok cat="NN">plasma</tok> <tok cat="NN">cortisol</tok> <tok cat="NNS">concentrations</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" syn="COOD" role="PRD"><cons cat="ADJP"><tok cat="JJ">normal</tok></cons> <tok cat="CC">or</tok> <cons cat="ADJP"><tok cat="RB">slightly</tok> <tok cat="JJ">elevated</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@19-12-2010
851151905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Despite</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">wide</tok> <tok cat="NN">range</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mean</tok> <tok cat="NN">GR</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">count</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">affinity</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">MNC</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">septic</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">differ</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NNS">controls</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">glucocorticoids</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">still</tok></cons> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">effective</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">hemodynamic</tok> <tok cat="JJ">compensatory</tok> <tok cat="NN">phase</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">sepsis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@19-12-2010
851507501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="JJ">Transcriptional</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">macrophage</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i4" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">secretion</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">bioactive</tok> <tok cat="NN">macrophage</tok> <tok cat="JJ">colony-stimulating</tok> <tok cat="NN">factor</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
851507502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Mo</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <cons cat="VP"><tok cat="VBP">display</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">beta-chain</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-2R</tok> <tok cat="NN">alpha-chain</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP" syn="COOD"><tok cat="RB">not</tok> <cons cat="ADJP"><tok cat="JJ">constitutive</tok> <cons cat="PP" ref="i5" null="RNR"></cons></cons><tok cat="CC">but</tok> <cons cat="ADJP"><tok cat="JJ">inducible</tok> <cons cat="PP" ref="i5" null="RNR"></cons></cons></cons><cons cat="PP" id="i5"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
851507503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">IL-2</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="VBG">binding</tok> <tok cat="NN">site</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">macrophage</tok> <tok cat="NN">CSF</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">M-CSF</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">gene</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Mo</tok></cons></cons> <cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">accumulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">M-CSF</tok> <tok cat="NN">mRNA</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">subsequent</tok> <tok cat="NN">release</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">bioactive</tok> <tok cat="NN">M-CSF</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons> <cons cat="SBAR"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">ELISA</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="NN">IL-2</tok> <tok cat="JJ">induced</tok></cons> <tok cat="NN">release</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">activity-stimulating</tok> <tok cat="NN">growth</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">monocyte-type</tok> <tok cat="NNS">colonies</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="VBG">neutralizing</tok> <tok cat="JJ">anti-M-CSF</tok> <tok cat="NN">antibody</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@62@@oe@19-12-2010
851507504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i7" role="SBJ"><cons cat="NP"><tok cat="JJ">Transcriptional</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">M-CSF</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">preceded</tok> <cons cat="NP" ref="i7" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="VBN">enhanced</tok> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">recognition</tok> <tok cat="NN">sequence</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">5'</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">enhancer</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">M-CSF</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@19-12-2010
851507505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">heterologous</tok> <tok cat="NN">promoter</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">herpes</tok> <tok cat="NN">thymidine</tok> <tok cat="NN">kinase</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">construct</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">consensus</tok> <tok cat="NN">sequence</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">IL-2-induced</tok> <tok cat="JJ">monocyte-derived</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">confers</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">growth</tok> <tok cat="NN">hormone</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@19-12-2010
851507506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" ref="i100" id="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">Taken</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">together</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i100" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-2</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">M-CSF</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">blood-derived</tok> <tok cat="NN">Mo</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
852236101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Synergy</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">transduction</tok> <tok cat="NNS">pathways</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">obligatory</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-fos</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">B</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">implication</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">surface</tok> <tok cat="NN">immunoglobulin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">antigen</tok> <tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@31@@oe@19-12-2010
852236102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">protooncogene</tok> <tok cat="NN">c-fos</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">controlled</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="CD">three</tok> <tok cat="JJ">main</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="VP"><tok cat="VBG">involving</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">cAMP</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">calcium</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@19-12-2010
852236103@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Kinase</tok> <tok cat="NN">C</tok></cons> <cons cat="VP"><tok cat="VBZ">mediates</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">serum</tok> <tok cat="NN">response</tok> <tok cat="NN">factor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SRF</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">interacts</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">serum</tok> <tok cat="NN">response</tok> <tok cat="NN">element</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SRE</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">cAMP</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">calcium</tok></cons></cons> <cons cat="VP"><tok cat="VBP">mediate</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CREB</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">cAMP</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">element</tok> <tok cat="NN">binding</tok> <tok cat="NN">protein</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">presumably</tok></cons> <tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">A</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">calmodulin-regulated</tok> <tok cat="NN">kinase</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@54@@oe@19-12-2010
852236104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">examined</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">elements</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">Burkitt</tok><tok cat="POS">'s</tok> <tok cat="NN">lymphoma</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Ramos</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Daudi</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="JJ">lymphocytic</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Jurkat</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
852236105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">any</tok> <tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">pathways</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">little</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">no</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">effect</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="NN">induction</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
852236106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PMA</tok> <tok cat="NN">stimulation</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBN">combined</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cAMP</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">forskolin</tok></cons> <tok cat="CC">plus</tok> <cons cat="NP"><tok cat="NN">MIX</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">calcium</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ionophore</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">leads</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">greatly</tok> <tok cat="VBN">enhanced</tok></cons> <cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="NN">induction</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
852236107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">cAMP</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">calcium</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">shows</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">synergy</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="NN">induction</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
852236108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i18" role="SBJ"><tok cat="JJ">Okadaic</tok> <tok cat="NN">acid</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">augments</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="NN">PMA-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="ADJP"><tok cat="JJ">calcium-mediated</tok></cons></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-fos</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">little</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">no</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NN">effect</tok></cons></cons> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i6"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">combined</tok> <cons cat="NP" ref="i18" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">cAMP</tok></cons></cons><cons cat="ADVP" ref="i6" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
852236109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">main</tok> <tok cat="NN">difference</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">Ramos</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Jurkat</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-fos</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="SBAR" role="PRD"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">cAMP</tok></cons> <tok cat="CC">plus</tok> <cons cat="NP"><tok cat="NN">calcium</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">strongly</tok> <tok cat="JJ">synergistic</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="PP" role="PRD"><tok cat="IN">without</tok> <cons cat="NP"><tok cat="NN">effect</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Ramos</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@19-12-2010
852236110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="NN">activity</tok></cons></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="NN">gel</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">confirms</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">requirements</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">synergy</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-fos</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="NN">induction</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">paralleled</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">requirements</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">synergy</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
852236111@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Signaling</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">due</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">anti-Ig</tok></cons> <tok cat="NN">stimulation</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">involves</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons> <tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">release</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">calcium</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-fos</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="NN">induction</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
852236112@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">synergy</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons> <tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">calcium</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">needed</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">efficient</tok> <cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="NN">induction</tok></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">since</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">neither</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="CD">two</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><tok cat="NN">c-fos</tok></cons> <cons cat="ADVP"><tok cat="RB">well</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
852236113@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" id="i10" func="NOM" role="SBJ"><tok cat="IN">That</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">synergy</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">relevant</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">anti-Ig</tok></cons> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-fos</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">supported</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="NN">fact</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="JJ">cAMP-inducing</tok> <tok cat="NNS">agents</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">okadaic</tok> <tok cat="NN">acid</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">further</tok></cons> <cons cat="VP"><tok cat="VBP">enhance</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">anti-Ig</tok></cons> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-fos</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
852236114@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">cell-specific</tok> <tok cat="NNS">patterns</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">synergy</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">essential</tok> <tok cat="NN">feature</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="NN">induction</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">relevant</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">c-fos</tok></cons> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">B</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NN">cell</tok> <tok cat="NN">antigen</tok> <tok cat="NNS">receptors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
852910801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Hemoglobin</tok></cons> <tok cat="NN">switching</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">humans</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">accompanied</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ratio</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">GATA-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">SP1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@19-12-2010
852910802@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">BACKGROUND</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="S" func="NOM" role="SBJ"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Understanding</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">developmental</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">hemoglobin</tok></cons> <tok cat="NN">switching</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">scientific</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">clinical</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">relevance</tok></cons></cons> <cons cat="PP"><tok cat="IN">because</tok> <tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">influence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">fetal</tok> <cons cat="NP"><tok cat="NN">hemoglobin</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HbF</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">adulthood</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">clinical</tok> <tok cat="NN">manifestation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">thalassemia</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">sickle</tok> <tok cat="NN">cell</tok> <tok cat="NN">anemia</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@42@@oe@19-12-2010
852910803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">normal</tok> <tok cat="JJ">developmental</tok> <tok cat="NNS">patterns</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">globin</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">recapitulated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">experimental</tok> <tok cat="NN">system</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NNS">cultures</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">support</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NNS">progenitors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
852910804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">further</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">activities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NNS">activators</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">GATA-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">SP1</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="JJ">adult</tok> <tok cat="JJ">erythroid</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
852910805@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">MATERIALS</tok></cons> <tok cat="CC">AND</tok> <cons cat="NP"><tok cat="NNS">METHODS</tok></cons><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">work</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">studied</tok><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">activities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">GATA-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">SP1</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">cultured</tok> <cons cat="NP"><tok cat="JJ">erythroid</tok> <tok cat="NNS">progenitors</tok></cons></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">cord</tok> <tok cat="NN">blood</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">fetal</tok> <tok cat="NNS">livers</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">beta</tok> <tok cat="JJ">zero-thalassemia</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@19-12-2010
852910806@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">RESULTS</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">high</tok> <cons cat="NP"><tok cat="NN">GATA-1</tok></cons> <tok cat="NN">binding</tok> <tok cat="NN">activity</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">very</tok> <tok cat="JJ">low</tok></cons> <cons cat="NP"><tok cat="NN">SP1</tok></cons> <tok cat="NN">activity</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">fetal</tok> <tok cat="NN">liver</tok> <tok cat="NNS">cultures</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
852910807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">pattern</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="PP" role="PRD"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">contrast</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">cultures</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">normal</tok> <tok cat="JJ">adult</tok></cons> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GATA-1</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">SP1</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NNS">activities</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">high</tok><cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
852910808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Cord</tok> <tok cat="NN">blood</tok> <tok cat="NNS">cultures</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">additive</tok> <tok cat="NN">combination</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="LQT">"</tok><tok cat="JJ">adult</tok><tok cat="RQT">"</tok></cons> <cons cat="NP" ref="i27" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="LQT">"</tok><tok cat="JJ">fetal</tok><tok cat="RQT">"</tok></cons> <cons cat="NP" ref="i27" null="RNR"></cons></cons><cons cat="NP" id="i27"><tok cat="NNS">patterns</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
852910809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">progenitors</tok></cons> <cons cat="VP"><tok cat="VBN">derived</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">beta</tok> <tok cat="JJ">zero-thalassemia</tok> <tok cat="NN">patient</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">high</tok> <cons cat="NP"><tok cat="NN">HbF</tok></cons> <tok cat="NN">production</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><tok cat="LQT">"</tok><tok cat="JJ">fetal</tok><tok cat="RQT">"</tok> <tok cat="NN">pattern</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
852910810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">On</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">other</tok> <tok cat="NN">hand</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cultures</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">2</tok> <tok cat="NN">beta</tok> <tok cat="JJ">zero-thalassemia</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP"><tok cat="JJ">high</tok> <cons cat="NP"><tok cat="NN">HbF</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><tok cat="LQT">"</tok><tok cat="JJ">adult</tok><tok cat="RQT">"</tok> <tok cat="NN">pattern</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok><cons cat="NP" ref="i9" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
852910811@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">CONCLUSIONS</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">work</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="JJ">human</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">fetal</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">adult</tok></cons> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="JJ">erythroid</tok> <tok cat="NNS">progenitors</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">distinct</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">commitment</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">fetal</tok></cons> <cons cat="NP" ref="i24" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">adult</tok></cons> <cons cat="NP" ref="i24" null="RNR"></cons></cons><cons cat="NP" id="i24"><tok cat="NN">program</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">very</tok> <tok cat="JJ">early</tok></cons> <tok cat="NN">differentiation</tok> <tok cat="NN">stage</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@19-12-2010
852910812@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">studies</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><tok cat="JJ">anemic</tok> <tok cat="NN">stress</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">recruitment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">fetal</tok> <tok cat="NNS">progenitors</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">occur</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">adulthood</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
852965701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">Transcriptional</tok></cons> <cons cat="NP"><tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">repression</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">properties</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">lymphoid-specific</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="NP"><tok cat="NN">Oct-2a</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@19-12-2010
852965702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">lymphoid-specific</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="NP"><tok cat="NN">Oct-2a</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok> <tok cat="NNS">domains</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="ADJP"><cons cat="NP"><tok cat="JJ">N-terminal</tok></cons></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">C-terminal</tok> <tok cat="NNS">regions</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@19-12-2010
852965703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">study</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">differential</tok> <tok cat="NN">activation</tok> <tok cat="NNS">properties</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">linked</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBN">isolated</tok> <cons cat="NP"><tok cat="NN">effector</tok> <tok cat="NNS">domains</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GAL4</tok> <tok cat="JJ">DNA-binding</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
852965704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">both</tok> <tok cat="VBG">activating</tok> <tok cat="NNS">regions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Oct-2a</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">natural</tok> <tok cat="NN">context</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
852965705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">contrast</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">C-terminus</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">N-terminal</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">yet</tok> <tok cat="JJ">unidentified</tok></cons> <tok cat="NN">factor</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons> <cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">simian</tok> <tok cat="NN">virus</tok> <tok cat="CD">40</tok> <tok cat="NN">enhancer</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
852965706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="NN">duplication</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">activation</tok> <tok cat="NNS">domains</tok></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">mixed</tok> <tok cat="NN">combination</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NNS">domains</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">functionally</tok> <tok cat="JJ">independent</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
852965707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">remote</tok> <tok cat="NN">position</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="ADVP"><tok cat="RB">only</tok></cons> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">achieved</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">C-terminus</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Oct-2a</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
852965708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">lymphoid</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><tok cat="JJR">higher</tok> <tok cat="NN">activation</tok> <tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">observed</tok><cons cat="NP" ref="i6" null="NONE"></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="JJ">distinct</tok> <cons cat="NP"><tok cat="JJ">B-cell-specific</tok> <tok cat="NNS">cofactors</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">concert</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">effector</tok> <tok cat="NNS">domains</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Oct-2a</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">mediating</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">proximal</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">remote</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">positions</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@19-12-2010
852965709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">repression</tok> <tok cat="NN">domain</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">N-terminus</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Oct-2a</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@19-12-2010
852965710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i9"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">transferred</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potent</tok> <tok cat="NN">activator</tok></cons></cons><cons cat="ADVP" ref="i9" null="T"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="JJ">transcriptional</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">efficiently</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@19-12-2010
852965711@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">underscore</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">modular</tok> <tok cat="NN">structure</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Oct-2a</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">separable</tok> <tok cat="NNS">domains</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">repression</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Oct-2a</tok></cons> <cons cat="VP"><tok cat="MD">might</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><tok cat="JJ">complex</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">functions</tok></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
853015601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Circumvention</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tolerance</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">protein</tok></cons> <cons cat="NP"><tok cat="NN">TCF-1</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">immunization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">TCF-1</tok></cons> <tok cat="JJ">knock-out</tok> <tok cat="NNS">mice</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@19-12-2010
853015602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="JJ">Molecular</tok> <tok cat="NNS">events</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">underlie</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD" role="PRD"><cons cat="ADJP"><tok cat="JJ">well-defined</tok></cons> <cons cat="ADJP"><tok cat="JJ">phenotypic</tok></cons></cons> <tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="VBG">differentiating</tok> <tok cat="NN">thymocyte</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">poorly</tok></cons> <cons cat="VP"><tok cat="VBN">understood</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@19-12-2010
853015603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">candidate</tok> <tok cat="NN">gene</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i3" null="ZERO"></cons><cons cat="S"><cons cat="NP" ref="i3" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">control</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">thymocyte</tok></cons> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">factor-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TCF-1</tok></cons><tok cat="RRB">)</tok><tok cat="SYM">*</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">encodes</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NN">protein</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
853015604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Its</tok> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok> <tok cat="NN">pattern</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" syn="COOD" role="PRD"><cons cat="ADJP"><tok cat="JJ">complex</tok> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">embryogenesis</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="RB">yet</tok> <cons cat="ADJP"><tok cat="JJ">restricted</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="ADVP"><tok cat="RB">postnatally</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
853015605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok> <tok cat="NNS">studies</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">TCF-1</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">hampered</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">difficulty</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">raise</tok> <cons cat="NP"><tok cat="NNS">antibodies</tok></cons></cons></cons></cons> <cons cat="ADJP"><tok cat="JJ">due</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">extreme</tok> <tok cat="JJ">evolutionary</tok></cons> <tok cat="NN">conservation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
853015606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">TCF-1</tok></cons> <tok cat="JJ">knock-out</tok> <tok cat="NNS">mice</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">generated</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">our</tok> <tok cat="NN">laboratory</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBN">decreased</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">numbers</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">thymocytes</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">otherwise</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">normal</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
853015607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">mice</tok></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">generate</tok> <cons cat="NP"><tok cat="NN">anti-TCF-1</tok> <tok cat="NNS">antibodies</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@19-12-2010
853015608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">immunization</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">recombinant</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">TCF-1</tok></cons> <tok cat="JJ">knock-out</tok> <tok cat="NNS">mice</tok></cons> <cons cat="ADVP"><tok cat="RB">readily</tok></cons> <cons cat="VP"><tok cat="VBP">yield</tok> <cons cat="NP"><tok cat="NN">antiserum</tok> <tok cat="NNS">titers</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">human</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">mouse</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">TCF-1</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
853015609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Wild-type</tok> <tok cat="NNS">littermates</tok></cons> <cons cat="VP"><tok cat="VBP">remain</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unresponsive</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">TCF-1</tok></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBP">mount</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">high-titer</tok> <tok cat="NN">antibody</tok> <tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">fusion</tok> <tok cat="NN">protein</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Maltose</tok> <tok cat="NN">Binding</tok> <tok cat="NN">Protein</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">MBP</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
853015610@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Subsequently</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><tok cat="JJ">TCF-1-specific</tok> <tok cat="NNS">hybridomas</tok></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">prepared</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">spleens</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">immunized</tok> <tok cat="JJ">knock-out</tok> <tok cat="NNS">mice</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
853015611@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">study</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">illustrates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">almost</tok> <tok cat="JJ">complete</tok></cons> <tok cat="NN">tolerance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">mice</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">TCF-1</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">demonstrates</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">tolerance</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">readily</tok></cons> <cons cat="VP"><tok cat="VBN">broken</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">gene</tok> <tok cat="NN">knock-out</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
853015612@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">usefulness</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">knock-out</tok> <tok cat="NNS">mice</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">generation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">monoclonal</tok> <tok cat="NNS">antibodies</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">gene</tok> <tok cat="NN">product</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">interest</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">underscored</tok><cons cat="NP" ref="i10" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
853048301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Solution</tok> <tok cat="NN">structure</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">sequence-specific</tok> <tok cat="NN">HMG</tok> <tok cat="NN">box</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lymphocyte</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activator</tok></cons> <cons cat="NP"><tok cat="NN">Sox-4</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@19-12-2010
853048302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="CD">Two</tok> <tok cat="NNS">groups</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HMG</tok> <tok cat="NN">box</tok> <tok cat="NNS">proteins</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">distinguished</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@9@@oe@19-12-2010
853048303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Proteins</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NN">group</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">contain</tok> <cons cat="NP"><tok cat="JJ">multiple</tok> <cons cat="NP"><tok cat="NN">HMG</tok> <tok cat="NNS">boxes</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">non-sequence-specific</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">recognize</tok> <cons cat="NP"><tok cat="JJ">structural</tok> <tok cat="NNS">features</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">cruciform</tok> <tok cat="NN">DNA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">cross-over</tok> <tok cat="NN">DNA</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
853048304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">abundant</tok> <cons cat="NP"><tok cat="JJ">chromosomal</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="NP"><tok cat="NN">HMG-1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">belongs</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">subgroup</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@19-12-2010
853048305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Proteins</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">second</tok> <tok cat="NN">group</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">carry</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">single</tok> <tok cat="NN">HMG</tok> <tok cat="NN">box</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">affinity</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">minor</tok> <tok cat="NN">groove</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">heptamer</tok> <tok cat="NN">motif</tok></cons> <cons cat="NP"><tok cat="NN">AACAAAG</tok></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">variations</tok></cons> <cons cat="ADVP"><tok cat="RB">thereof</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
853048306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">solution</tok> <tok cat="NN">structure</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">non-sequence-specific</tok> <cons cat="NP"><tok cat="JJ">C-terminal</tok> <tok cat="NN">HMG</tok> <tok cat="NN">box</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HMG-1</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">proposed</tok><cons cat="NP" ref="i6" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
853048307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Now</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">solution</tok> <tok cat="NN">structure</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">sequence-specific</tok> <tok cat="NN">HMG-box</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">SRY-related</tok> <tok cat="NN">protein</tok></cons> <cons cat="NP"><tok cat="NN">Sox-4</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
853048308@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NMR</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok> <cons cat="NP"><tok cat="NNS">alpha-helices</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Val10-Gln22</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Glu30-Leu41</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Phe50-Tyr65</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBN">connected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">loop</tok> <tok cat="NNS">regions</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Ser23-Ala49</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Leu42-Pro49</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
853048309@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="NNS">Helices</tok> <cons cat="NP"><tok cat="CD">I</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">II</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">positioned</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">antiparallel</tok> <tok cat="NN">mode</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">form</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok> <tok cat="NN">arm</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">HMG</tok> <tok cat="NN">box</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
853048310@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Helix</tok> <tok cat="CD">III</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="RBR">less</tok> <tok cat="JJ">rigid</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBZ">makes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">average</tok> <tok cat="NN">angle</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="QP"><tok cat="RB">about</tok> <tok cat="CD">90</tok></cons> <tok cat="NNS">degrees</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><tok cat="NNS">helices</tok> <cons cat="NP"><tok cat="CD">I</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">II</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">constitutes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">other</tok> <tok cat="NN">arm</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">molecule</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
853048311@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">As</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">HMG1B</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">overall</tok> <tok cat="NN">structure</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Sox-4</tok></cons> <cons cat="NP"><tok cat="NN">HMG</tok> <tok cat="NN">box</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">L-shaped</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">maintained</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cluster</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="VBN">conserved</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="RB">mainly</tok> <tok cat="JJ">aromatic</tok></cons></cons> <tok cat="NNS">residues</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
853735401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">A</tok> <cons cat="NP"><tok cat="NN">PEBP2</tok> <tok cat="JJ">alpha/AML-1-related</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">increases</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">osteocalcin</tok> <tok cat="NN">promoter</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">osteoblast-specific</tok> <tok cat="JJ">cis-acting</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@19-12-2010
853735402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">identify</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">osteoblast-specific</tok> <tok cat="JJ">cis-acting</tok> <tok cat="NNS">elements</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">trans-acting</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">initiated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">promoter</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">mouse</tok> <tok cat="NN">osteocalcin</tok> <tok cat="NN">gene</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">osteoblast-specific</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@26@@oe@19-12-2010
853735403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">promoter</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><tok cat="CD">two</tok> <cons cat="NP"><tok cat="JJ">osteoblast-specific</tok> <tok cat="JJ">cis-acting</tok> <tok cat="NNS">elements</tok></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNP">Ducy</tok><tok cat="COMMA">,</tok> <tok cat="NNP">P.</tok></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNP">Karsenty</tok><tok cat="COMMA">,</tok> <tok cat="NNP">G.</tok></cons></cons><cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="CD">1995</tok></cons><tok cat="RRB">)</tok></cons> <cons cat="NP"><tok cat="NNP">Mol.Cell.Biol.15</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">1858-1869</tok></cons></cons><tok cat="RRB">)</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
853735404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">sequence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NNS">elements</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">OSE2</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">identical</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">PEBP2</tok> <tok cat="NN">alpha/AML-1</tok> <tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">mammalian</tok> <tok cat="NNS">homologues</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="FW">Drosophila</tok> <tok cat="NN">Runt</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
853735405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" id="i5" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">recombinant</tok> <tok cat="NN">protein</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">specific</tok> <tok cat="NN">antiserum</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="NN">AML-1</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NNS">assays</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok> <tok cat="NN">member</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">family</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">AML-1B</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">OSE2</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">immunologically</tok> <tok cat="JJ">related</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">OSF2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">factor</tok></cons> <cons cat="ADJP"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">osteoblast</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">OSE2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@54@@oe@19-12-2010
853735406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">cotransfection</tok> <tok cat="NNS">experiments</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">AML-1B</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">increase</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">short</tok> <cons cat="NP"><tok cat="NN">osteocalcin</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">OSE2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
853735407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Lastly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">different</tok> <tok cat="NNS">mobilities</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">osteoblast</tok> <tok cat="JJ">nuclear</tok> <tok cat="JJ">extract-DNA</tok> <tok cat="NNS">complexes</tok></cons> <cons cat="PP"><tok cat="VBN">compared</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="JJ">nuclear</tok> <tok cat="JJ">extract-DNA</tok> <tok cat="NNS">complexes</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="PP"><tok cat="IN">along</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">inability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">OSF2</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">upregulated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">unlike</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">other</tok> <cons cat="NP"><tok cat="NN">PEBP2</tok> <tok cat="NN">alpha</tok> <tok cat="NNS">factors</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">OSF2</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">new</tok> <tok cat="NN">member</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">family</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@51@@oe@19-12-2010
853735408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">demonstrates</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">AML-1B</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">increase</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">osteoblast-specific</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="NP"><tok cat="JJ">osteoblast-specific</tok> <tok cat="JJ">cis-acting</tok> <tok cat="NN">element</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">presents</tok> <cons cat="NP"><tok cat="NN">evidence</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">OSF2</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">member</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">PEBP2</tok> <tok cat="NN">alpha/AML-1</tok> <tok cat="NN">family</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@19-12-2010
854820001@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="JJ">human</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">elevated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">zinc</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="NN">trace</tok> <tok cat="NN">element</tok> <tok cat="NN">preparation</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@19-12-2010
854820002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">trace</tok> <tok cat="NN">element</tok> <tok cat="NN">preparation</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">Beres</tok> <tok cat="NN">Drops</tok> <tok cat="NN">Plus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">BDP</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">elevates</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">gcR</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="CC">both</tok> <tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">healthy</tok> <tok cat="NN">blood</tok> <tok cat="NNS">donors</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">rheumatoid</tok> <tok cat="NN">arthritis</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@34@@oe@19-12-2010
854820003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">This</tok> <tok cat="NN">enhancement</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">BDP</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP" syn="COOD"><tok cat="CC">either</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">constitutive</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">gcRs</tok></cons></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">experiments</tok></cons></cons></cons></cons> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i7"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">interleukin</tok> <tok cat="LRB">(</tok><tok cat="NN">IL</tok><tok cat="RRB">)</tok><tok cat="CD">-6</tok></cons></cons><cons cat="ADVP" ref="i7" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
854820004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">There</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="JJ">significant</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">BDP</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">tumour</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor</tok> <tok cat="NN">alpha</tok></cons> <tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TNF</tok> <tok cat="NN">alpha</tok></cons><tok cat="RRB">)</tok><tok cat="JJ">-induced</tok> <tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">gcRs</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
854820005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">BDP</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">greatly</tok> <tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Zn++</tok> <tok cat="NNS">ions</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">preparation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">since</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="DT">the</tok> <tok cat="VBG">augmenting</tok> <tok cat="NN">effect</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">abolished</tok> <cons cat="NP" ref="i10" null="NONE"></cons></cons><cons cat="SBAR" func="ADV"><tok cat="IN">if</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">BDP</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">contain</tok> <cons cat="NP"><tok cat="NN">zinc</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
855079701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">lipopolysaccharide-induced</tok> <cons cat="NP"><tok cat="NN">monocyte</tok> <tok cat="NN">interleukin-1</tok> <tok cat="NN">receptor</tok></cons> <tok cat="NN">antagonist</tok> <tok cat="NN">synthesis</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">cortisol</tok></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">mineralocorticoid</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@19-12-2010
855079702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">Glucocorticoids</tok><tok cat="COMMA">,</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">part</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">physiological</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">inflammatory</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">immune</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">processes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBP">suppress</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">other</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@30@@oe@19-12-2010
855079703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i5" role="SBJ"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Human</tok> <tok cat="NN">monocyte</tok> <tok cat="NN">IL-1</tok> <tok cat="NN">receptor</tok> <tok cat="NN">antagonist</tok> <tok cat="LRB">(</tok><tok cat="NN">IL-1ra</tok><tok cat="RRB">)</tok> <tok cat="NN">messenger</tok> <tok cat="NN">ribonucleic</tok> <tok cat="NN">acid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">mRNA</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">expression</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">secretion</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">dexamethasone</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
855079704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">now</tok></cons> <cons cat="ADVP"><tok cat="RB">further</tok></cons> <cons cat="VP"><tok cat="VBN">studied</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-1ra</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">major</tok></cons> <cons cat="ADJP"><tok cat="JJ">physiological</tok></cons></cons> <tok cat="JJ">human</tok> <tok cat="NN">glucocorticoid</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">cortisol</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
855079705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">cortisol</tok> <tok cat="NN">incubation</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-1ra</tok> <tok cat="NN">mRNA</tok></cons> <tok cat="NN">expression</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">significant</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-1ra</tok> <tok cat="NN">protein</tok></cons> <tok cat="NN">secretion</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NNS">cultures</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NNS">monocytes</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">bacterial</tok> <tok cat="NN">endotoxin</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lipopolysaccharide</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LPS</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@19-12-2010
855079706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Oral</tok> <tok cat="NN">administration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="CD">276</tok> <tok cat="NN">mumol</tok></cons> <tok cat="NN">cortisol</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NNS">subjects</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">decreased</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">LPS-induced</tok> <cons cat="NP"><tok cat="NN">IL-1ra</tok></cons> <tok cat="NN">synthesis</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">cultured</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
855079707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">coincubating</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mineralocorticoid</tok> <tok cat="NN">antagonist</tok> <tok cat="NN">spironolactone</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons> <tok cat="NN">antagonist</tok> <tok cat="NN">RU</tok> <tok cat="CD">38486</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <cons cat="ADJP"><tok cat="JJ">cortisol-induced</tok></cons></cons> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">LPS-stimulated</tok> <cons cat="NP"><tok cat="NN">IL-1ra</tok></cons> <tok cat="NN">secretion</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">partially</tok></cons> <cons cat="VP"><tok cat="VBN">reversed</tok><cons cat="NP" ref="i6" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
855079708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">mineralocorticoid</tok> <tok cat="NN">aldosterone</tok></cons> <cons cat="VP"><tok cat="VBD">exerted</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">significant</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">LPS-induced</tok> <tok cat="NN">monocyte</tok> <cons cat="NP"><tok cat="NN">IL-1ra</tok></cons> <tok cat="NN">secretion</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" id="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">coincubation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">spironolactone</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
855079709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">mineralocorticoid</tok> <tok cat="NN">receptor</tok> <tok cat="NN">mRNA</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">PCR</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">reversed</tok> <tok cat="VBN">transcribed</tok> <tok cat="NN">RNA</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
855079710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="ADVP"><tok cat="RB">further</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">corticosteroids</tok></cons> <cons cat="ADVP"><tok cat="RB">physiologically</tok></cons> <cons cat="VP"><tok cat="VBP">control</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">IL-1/IL-1ra</tok> <tok cat="NN">system</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">inflammatory</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">immune</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">processes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
855079711@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><tok cat="NN">evidence</tok> <cons cat="SBAR"><tok cat="IN">that</tok><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">glucocorticoid</tok> <tok cat="JJ">receptor-mediated</tok> <tok cat="NN">effect</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">mineralocorticoid</tok> <tok cat="NN">receptor</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">monocyte</tok> <cons cat="NP"><tok cat="NN">IL-1ra</tok></cons> <tok cat="NN">secretion</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">cortisol</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
855209701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">E2F-1</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">blocks</tok> <cons cat="NP"><tok cat="JJ">terminal</tok> <tok cat="NN">differentiation</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">causes</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">proliferation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">transgenic</tok> <tok cat="NNS">megakaryocytes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@11@@oe@19-12-2010
855209702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="NP"><tok cat="NN">E2F-1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">central</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">cycle</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">genes</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NN">division</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@19-12-2010
855209703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">E2F-1</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">proteins</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">retinoblastoma</tok> <cons cat="NP"><tok cat="NN">susceptibility</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">product</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">cyclin-dependent</tok> <tok cat="NNS">kinases</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="PRPP">their</tok> <cons cat="NP"><tok cat="NNS">inhibitors</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNS">proteins</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" id="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">certain</tok> <tok cat="JJ">developmental</tok> <tok cat="NNS">processes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@19-12-2010
855209704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i12" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">investigate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potential</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">E2F-1</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">assayed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">megakaryocytes</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">form</tok> <cons cat="NP"><tok cat="NNS">platelets</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons> <tok cat="JJ">transgenic</tok> <tok cat="NN">model</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
855209705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">E2F-1</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">megakaryocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">blocked</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><tok cat="NN">maturation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">resulting</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">severe</tok> <tok cat="NN">thrombocytopenia</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
855209706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Ultrastructural</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">megakaryocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">abnormal</tok> <tok cat="NN">development</tok></cons> <cons cat="VP"><tok cat="VBN">characterized</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="NN">hyperdemarcation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">cytoplasmic</tok> <tok cat="NNS">membranes</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">reduced</tok> <tok cat="NNS">numbers</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">alpha</tok> <tok cat="NNS">granules</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
855209707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Administration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">megakaryocyte</tok> <tok cat="NN">growth</tok> <tok cat="CC">and</tok> <tok cat="NN">development</tok> <tok cat="NN">factor</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="CD">6</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">overcome</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">differentiation</tok> <tok cat="NN">block</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
855209708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Additionally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">E2F-1</tok></cons> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">massive</tok> <cons cat="NP"><tok cat="NN">megakaryocyte</tok></cons> <tok cat="NN">accumulation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">normal</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">ectopic</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">sites</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><cons cat="ADVP"><tok cat="RB">first</tok></cons> <tok cat="JJ">evident</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="CD">E15</tok> <tok cat="JJ">embryonic</tok> <tok cat="NN">liver</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
855209709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i13" role="SBJ"><tok cat="JJ">significant</tok> <tok cat="NN">apoptosis</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">transgenic</tok> <tok cat="NNS">megakaryocytes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@19-12-2010
855209710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">E2F-1</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">prevent</tok> <cons cat="NP"><tok cat="JJ">terminal</tok> <tok cat="NN">differentiation</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">probably</tok></cons> <tok cat="IN">through</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">cell</tok> <tok cat="JJ">cycle-stimulatory</tok> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
855445101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Immunophenotype</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">intraductal</tok> <tok cat="NN">carcinoma</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@5@@oe@19-12-2010
855445102@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">OBJECTIVE</tok><tok cat="COLON">--</tok></cons><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NN">Mammography</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">breast-conserving</tok> <tok cat="NN">therapy</tok></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">focused</tok> <cons cat="NP"><tok cat="NN">attention</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">classification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">intraductal</tok> <tok cat="NN">carcinoma</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IDC</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">emphasized</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">prognostic</tok> <tok cat="NN">importance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">comedo</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">versus</tok> <cons cat="NP"><tok cat="JJ">noncomedo</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">variants</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@29@@oe@19-12-2010
855445103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">used</tok> <cons cat="NP"><tok cat="JJ">histochemical</tok> <tok cat="NNS">markers</tok></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">define</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">immunophenotype</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">43</tok> <tok cat="NNS">IDCs</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">respect</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">comedo</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">versus</tok> <cons cat="NP"><tok cat="JJ">noncomedo</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NN">status</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patterns</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">angiogenesis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
855445104@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">RESULTS</tok><tok cat="COLON">--</tok></cons><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Reactions</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">comedo</tok> <tok cat="NNS">carcinomas</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" syn="COOD" role="PRD"><cons cat="ADJP"><tok cat="RB">significantly</tok> <tok cat="JJ">negative</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">estrogen</tok> <tok cat="NN">receptor</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">progesterone</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">positive</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p53</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">HER-2/neu</tok></cons></cons></cons></cons> <cons cat="ADVP"><cons cat="ADVP"><tok cat="RBR">more</tok> <tok cat="RB">often</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">than</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">noncomedo</tok> <tok cat="NN">variant</tok></cons><cons cat="VP" null="QSTN"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
855445105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">All</tok> <tok cat="CD">seven</tok> <tok cat="NNS">IDCs</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">Paget</tok><tok cat="POS">'s</tok> <tok cat="NN">disease</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">positive</tok> <tok cat="NNS">reactions</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">HER-2/neu</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
855445106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Basement</tok> <tok cat="NN">membrane</tok> <tok cat="NN">immunoreactivity</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">type</tok> <tok cat="CD">IV</tok> <tok cat="NN">collagen</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">laminin</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">discontinuous</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJS">most</tok> <tok cat="NNS">examples</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IDC</tok></cons> <cons cat="ADVP"><tok cat="RB">regardless</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">type</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">trend</tok></cons> <cons cat="PP"><tok cat="IN">toward</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RBR">more</tok> <tok cat="JJ">intense</tok></cons> <tok cat="NN">staining</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">comedo</tok></cons></cons> <cons cat="PP"><tok cat="IN">than</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">noncomedo</tok> <tok cat="NNS">carcinomas</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@19-12-2010
855445107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Periductal</tok> <tok cat="NN">angiogenesis</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="RB">significantly</tok> <tok cat="JJ">related</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">type</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IDC</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="RBR">more</tok> <tok cat="JJ">pronounced</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">comedo</tok> <tok cat="NNS">carcinomas</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
855445108@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">CONCLUSIONS</tok><tok cat="COLON">--</tok></cons><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">observations</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">immunophenotypic</tok> <tok cat="NNS">correlates</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">current</tok></cons> <cons cat="ADJP"><tok cat="JJ">structural</tok></cons></cons> <tok cat="NN">classification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IDC</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
855445109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">immunophenotype</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IDC</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">helpful</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">subclassifying</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">IDC</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">prove</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">useful</tok> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">prognostic</tok> <tok cat="NN">indicator</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">local</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">patients</tok></cons> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">breast-conserving</tok> <tok cat="NN">therapy</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
855546401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Thrombopoietin</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Stat3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Stat5</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">blood</tok> <tok cat="NNS">platelets</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@19-12-2010
855546402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">Thrombopoietin</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">megakaryocytopoiesis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">thrombopoiesis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@11@@oe@19-12-2010
855546403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP" adv="TMP"><tok cat="RB">Recently</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="PRP">we</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">others</tok></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">thrombopoietin</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">rapid</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Jak2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">platelets</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">BaF3</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">genetically</tok></cons> <tok cat="VBN">engineered</tok> <cons cat="NP" null="NONE"></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">express</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-Mpl</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">thrombopoietin</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@19-12-2010
855546404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">Jak</tok> <tok cat="NN">family</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NNS">kinases</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">mediate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">some</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">cytokines</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NN">growth</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">recruitment</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">variety</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Stat</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">signal</tok> <tok cat="NNS">transducers</tok> <tok cat="CC">and</tok> <tok cat="NNS">activators</tok> <tok cat="IN">of</tok> <tok cat="NN">transcription</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@19-12-2010
855546405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Hence</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Stat</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">platelets</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">if</tok> <cons cat="FRAG"><cons cat="ADVP"><tok cat="RB">so</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" id="i33" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBP">become</tok> <cons cat="VP"><tok cat="NN">tyrosine</tok> <tok cat="JJ">phosphorylated</tok> <cons cat="NP" ref="i33" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">thrombopoietin</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
855546406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">immunologically</tok></cons> <cons cat="VP"><tok cat="VBD">identified</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Stat1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Stat2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Stat3</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Stat5</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">platelet</tok> <tok cat="NNS">lysates</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
855546407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Thrombopoietin</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Stat3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Stat5</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@19-12-2010
855546408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Thrombopoietin</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Stat3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Stat5</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">FDCP-2</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">genetically</tok></cons> <tok cat="VBN">engineered</tok> <cons cat="NP" null="NONE"></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="ADVP"><tok cat="RB">constitutively</tok></cons> <cons cat="VP"><tok cat="VB">express</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">c-Mpl</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
855546409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">Stat3</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Stat5</tok></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">ligand</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">c-Mpl</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">event</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">megakaryopoiesis/thrombopoiesis</tok></cons> <tok cat="CC">or</tok> <cons cat="ADVP"><tok cat="RB">possibly</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">mature</tok> <tok cat="NN">platelet</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">aggregation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@19-12-2010
855546701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Distinctive</tok> <tok cat="NN">expression</tok> <tok cat="NN">pattern</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">BCL-6</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">nodular</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NN">predominance</tok> <tok cat="NN">Hodgkin</tok><tok cat="POS">'s</tok> <tok cat="NN">disease</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@19-12-2010
855546702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">BCL-6</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">nuclear-located</tok> <tok cat="JJ">Kruppel-type</tok> <tok cat="NN">zinc</tok> <tok cat="NN">finger</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">rearranged</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><cons cat="QP"><tok cat="RB">about</tok> <tok cat="CD">30</tok></cons><tok cat="NN">%</tok></cons> <tok cat="JJ">diffuse</tok> <tok cat="JJ">large</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NNS">lymphomas</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">predominantly</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">normal</tok> <cons cat="NP"><tok cat="JJ">germinal</tok> <tok cat="NN">center</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">related</tok> <tok cat="NNS">lymphomas</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@34@@oe@19-12-2010
855546703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">BCL-6</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">normal</tok> <cons cat="NP"><tok cat="JJ">germinal</tok> <tok cat="NN">center</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="VBN">deregulated</tok> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBN">caused</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NNS">rearrangements</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">lymphomagenesis</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
855546704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBD">prompted</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">us</tok></cons> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">investigate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">BCL-6</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Hodgkin</tok><tok cat="POS">'s</tok> <tok cat="NN">disease</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HD</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">focusing</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nodular</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NN">predominance</tok> <tok cat="NN">subtype</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NLPHD</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">differs</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">classical</tok> <tok cat="NN">HD</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">virtue</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">B-cell</tok> <tok cat="NN">nature</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">malignant</tok> <tok cat="NN">cell</tok> <tok cat="NN">population</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">so-called</tok> <cons cat="NP"><tok cat="NN">L</tok><tok cat="CC">&amp;</tok><tok cat="NN">H</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">relationship</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="JJ">germinal</tok> <tok cat="NNS">centers</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@60@@oe@19-12-2010
855546705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="CD">Forty-one</tok> <tok cat="NN">HD</tok> <tok cat="NNS">samples</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">19</tok> <tok cat="NN">NLPHD</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">12</tok> <tok cat="JJ">nodular</tok> <tok cat="NN">sclerosis</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">10</tok> <tok cat="JJ">mixed</tok> <tok cat="NN">cellularity</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">immunostained</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">monoclonal</tok> <tok cat="NNS">antibodies</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PG-B6</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">PG-B6p</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">react</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">fixative-sensitive</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">formalin-resistant</tok> <tok cat="NN">epitope</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">aminoterminal</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">BCL-6</tok> <tok cat="NN">gene</tok> <tok cat="NN">product</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@19-12-2010
855546706@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="JJ">Strong</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">positivity</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">BCL-6</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="LRB">(</tok><tok cat="NN">L</tok><tok cat="CC">&amp;</tok><tok cat="NN">H</tok><tok cat="RRB">)</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">all</tok> <tok cat="NNS">cases</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NLPHD</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
855546707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><tok cat="NN">BCL-6</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">small</tok> <tok cat="NN">percentage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Hodgkin</tok> <tok cat="CC">and</tok> <tok cat="NN">Reed-Sternberg</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="RB">about</tok> <tok cat="CD">30</tok></cons><tok cat="NN">%</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">classical</tok> <tok cat="NN">HD</tok> <tok cat="NNS">cases</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
855546708@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Notably</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">nuclei</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">reactive</tok> <tok cat="JJ">CD3+/CD4+</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="UCP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">nearby</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBG">rosetting</tok> <cons cat="PP"><tok cat="IN">around</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons></cons><cons cat="NP" id="i20"><cons cat="NP"><tok cat="NN">L</tok><tok cat="CC">&amp;</tok><tok cat="NN">H</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">NLPHD</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="RB">strongly</tok> <tok cat="JJ">BCL-6+</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">lacked</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">CD40</tok> <tok cat="NN">ligand</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CD40L</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">expression</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@19-12-2010
855546709@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">staining</tok> <tok cat="NN">pattern</tok></cons> <cons cat="ADVP"><tok cat="RB">clearly</tok></cons> <cons cat="VP"><tok cat="VBD">differed</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">classical</tok> <tok cat="NN">HD</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i11"><tok cat="WPP">whose</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">background</tok></cons> <cons cat="S"><cons cat="NP" ref="i11" id="i12" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">made</tok> <cons cat="PRT"><tok cat="IN">up</tok></cons> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">CD3+/CD4+</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBG">showing</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">BCL-6-/CD40L+</tok> <tok cat="NN">phenotype</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
855546710@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="ADVP"><tok cat="RBR">further</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">support</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">concept</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NLPHD</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="RB">histogenetically</tok> <tok cat="JJ">distinct</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">B-cell-derived</tok></cons></cons> <tok cat="NN">subtype</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HD</tok></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">BCL-6</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">development</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
855639201@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="JJ">Selective</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">damaging</tok> <tok cat="NNS">agents</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok> <tok cat="NN">activation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">virus</tok> <tok cat="NN">replication</tok></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
855639202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Much</tok> <tok cat="NN">attention</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP"><tok cat="VBN">focused</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">observation</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">UV</tok> <tok cat="NN">light</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">long</tok> <tok cat="JJ">terminal</tok> <tok cat="NN">repeat</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LTR</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">immunodeficiency</tok> <tok cat="NN">virus</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HIV</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
855639203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">LTR</tok> <tok cat="NN">activation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">remains</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">obscure</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NNS">lines</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">investigation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">suggested</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">result</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="VBG">following</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">signaling</tok> <tok cat="NNS">events</tok></cons> <cons cat="ADJP"><tok cat="JJ">related</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="VBN">generalized</tok> <tok cat="NN">DNA</tok> <tok cat="NN">damage</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@19-12-2010
855639204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">report</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">present</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="NN">demonstrating</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HIV</tok> <tok cat="NN">LTR</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" syn="COOD" role="PRD"><tok cat="RB">not</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">general</tok> <tok cat="NN">consequence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NN">DNA</tok> <tok cat="NN">damage</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">rather</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">process</tok></cons> <cons cat="ADJP"><tok cat="JJ">unique</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <tok cat="JJ">genotoxic</tok> <tok cat="NNS">stimuli</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <cons cat="ADVP"><tok cat="RB">not</tok> <tok cat="RB">necessarily</tok></cons> <cons cat="VP"><tok cat="VB">depend</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@45@@oe@19-12-2010
855639205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">several</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">agents</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">increase</tok> <cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">replication</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">accelerate</tok> <cons cat="NP"><tok cat="JJ">CD4-positive</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NN">cytotoxicity</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
855639206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">have</tok> <cons cat="NP"><tok cat="JJ">clinical</tok> <tok cat="NN">significance</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">AIDS</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NNS">malignancies</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i81"><tok cat="WP">who</tok></cons> <cons cat="S"><cons cat="NP" ref="i81" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBG">undergoing</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">radiotherapy</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">chemotherapy</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
855703201@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">NF-M</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">chicken</tok> <tok cat="NN">C/EBP</tok> <tok cat="NN">beta</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">eosinophilic</tok> <tok cat="NN">differentiation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NN">progenitor</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@19-12-2010
855703202@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">CAAT/enhancer</tok> <tok cat="NN">binding</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">C/EBPs</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NNS">activators</tok></cons> <cons cat="VP"><tok cat="VBN">implicated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">differentiation</tok> <tok cat="NNS">processes</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">various</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lineages</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@19-12-2010
855703203@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="ADVP" adv="TMP"><tok cat="RBR">earlier</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">NF-M</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">chicken</tok> <tok cat="NN">homolog</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">C/EBP</tok> <tok cat="NN">beta</tok></cons><tok cat="COMMA">,</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">myelomonocytic</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">eosinophilic</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">hematopoietic</tok> <tok cat="NN">system</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
855703204@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">investigate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NF-M</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">hematopoietic</tok> <tok cat="NN">cell</tok> <tok cat="NN">lineage</tok></cons> <tok cat="NN">commitment</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">constructed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">conditional</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">fusing</tok> <cons cat="NP"><tok cat="PRP">it</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">hormone</tok> <tok cat="NN">binding</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">estrogen</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@19-12-2010
855703205@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">construct</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">stably</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">multipotent</tok> <tok cat="NN">progenitor</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons> <cons cat="VP"><tok cat="VBN">transformed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Myb-Ets</tok> <tok cat="NN">oncoprotein</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
855703206@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i8" role="SBJ"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="JJ">NF-M-dependent</tok> <tok cat="NN">promoter</tok> <tok cat="NNS">constructs</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">resident</tok> <tok cat="NNS">genes</tok></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">beta-estradiol</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">NF-M-estrogen</tok> <tok cat="NN">receptor</tok> <tok cat="VBG">expressing</tok> <tok cat="NNS">progenitors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
855703207@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">At</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NN">time</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">observed</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">down-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">progenitor-specific</tok> <tok cat="NN">surface</tok> <tok cat="NNS">markers</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">up-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">differentiation</tok> <tok cat="NNS">markers</tok></cons> <cons cat="ADJP"><tok cat="JJ">restricted</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">eosinophil</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">myeloid</tok> <tok cat="NNS">lineages</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
855703208@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">onset</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><tok cat="NN">cell</tok> <tok cat="NN">death</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">typical</tok></cons> <cons cat="ADJP"><tok cat="JJ">apoptotic</tok></cons></cons> <tok cat="NNS">features</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
855703209@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">NF-M</tok></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">commitment</tok></cons> <cons cat="PP"><tok cat="IN">along</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">eosinophil</tok> <tok cat="NN">lineage</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
855801101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HIV</tok> <tok cat="NN">glycoprotein</tok> <tok cat="CD">120</tok></cons> <cons cat="VP"><tok cat="VBZ">enhances</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">intercellular</tok> <tok cat="NN">adhesion</tok> <tok cat="NN">molecule-1</tok></cons> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">glial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@13@@oe@19-12-2010
855801102@GENIA Treebank@formal@@1@S@<cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">Involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Janus</tok> <tok cat="JJ">kinase/signal</tok> <tok cat="NN">transducer</tok> <tok cat="CC">and</tok> <tok cat="NN">activator</tok> <tok cat="IN">of</tok> <tok cat="NN">transcription</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
855801103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">well</tok></cons> <cons cat="VP"><tok cat="VBN">established</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">two</tok> <tok cat="JJ">major</tok> <cons cat="NP"><tok cat="JJ">glial</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">central</tok> <tok cat="JJ">nervous</tok> <tok cat="NN">system</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CNS</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">astrocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">microglia</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">key</tok> <tok cat="NNS">participants</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">mediating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">neurologic</tok> <tok cat="NN">dysfunction</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">HIV</tok> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CNS</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@39@@oe@19-12-2010
855801104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">major</tok> <tok cat="NN">envelope</tok> <tok cat="NN">glycoprotein</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HIV</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glycoprotein</tok> <tok cat="CD">120</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">gp120</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">intercellular</tok> <tok cat="NN">adhesion</tok> <tok cat="NN">molecule-1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ICAM-1</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">glial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">because</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">ICAM-1</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBG">mediating</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">immune</tok> <tok cat="NN">responsiveness</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CNS</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBG">facilitating</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">entry</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">HIV-infected</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">CNS</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBG">promoting</tok> <cons cat="NP"><tok cat="NN">syncytia</tok> <tok cat="NN">formation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@61@@oe@19-12-2010
855801105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">gp120</tok></cons> <cons cat="VP"><tok cat="VBZ">enhances</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">ICAM-1</tok></cons> <tok cat="NN">gene</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">rat</tok> <tok cat="NNS">astrocytes</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="JJ">human</tok> <tok cat="NNS">astrocytes</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">astroglioma</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">CRT</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">rat</tok> <tok cat="NNS">microglia</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
855801106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok> <tok cat="NNS">events</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">gp120-mediated</tok> <tok cat="NN">enhancement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ICAM-1</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">appear</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">involve</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">because</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">inhibitors</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">abrogate</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">gp120-mediated</tok> <cons cat="NP"><tok cat="NN">ICAM-1</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NNS">astrocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">microglia</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@19-12-2010
855801107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">gp120</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">transducer</tok> <tok cat="CC">and</tok> <tok cat="NN">activator</tok> <tok cat="IN">of</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">STAT-1</tok> <tok cat="NN">alpha</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Janus</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">JAK2</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">glial</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
855801108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">gp120-mediated</tok> <cons cat="NP"><tok cat="NN">ICAM-1</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="NP"><tok cat="JJ">functional</tok> <tok cat="NN">significance</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">enhances</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">monocytic</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">gp120-stimulated</tok> <tok cat="JJ">human</tok> <tok cat="NNS">astrocytes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">ICAM-1/beta</tok> <tok cat="CD">2</tok> <tok cat="JJ">integrin-dependent</tok> <tok cat="NN">fashion</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
855801109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">new</tok> <tok cat="NNS">insights</tok></cons> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="SBAR"><cons cat="WHADVP" id="i18"><tok cat="WRB">how</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">gp120</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">influence</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">glial</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">pathogenesis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AIDS</tok> <tok cat="JJ">dementia</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i18" null="T"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
856295501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Fas</tok></cons> <tok cat="NN">ligation</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Jun</tok> <tok cat="NN">kinase</tok></cons> <tok cat="NN">activation</tok></cons></cons> <cons cat="ADVP"><cons cat="ADVP"><tok cat="RB">independently</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CD45</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Lck</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@19-12-2010
856295502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Stimulation</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">Fas/APO-1</tok> <tok cat="NN">receptor</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="NNS">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP"><tok cat="RB">incompletely</tok> <tok cat="VBN">characterized</tok></cons> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@19-12-2010
856295503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="JJR">More</tok> <cons cat="VP" ref="i30" null="ICH"></cons></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="NP" ref="i5" null="NONE"></cons></cons></cons><cons cat="VP" id="i30"><tok cat="VBG">regarding</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">transduction</tok> <tok cat="NNS">events</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">occur</tok> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">ligation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T-cell</tok> <tok cat="NN">antigen</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TCR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
856295504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">TCR</tok></cons> <tok cat="NN">stimulation</tok></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">membrane</tok> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphatase</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CD45</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">Src-family</tok> <tok cat="NN">kinase</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Lck</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">result</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
856295505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">prior</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">tyrosine</tok> <tok cat="NNS">kinases</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">phosphatases</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Fas</tok></cons> <tok cat="NN">signaling</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Fas</tok> <tok cat="NN">ligation</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">apoptosis</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="ADJP"><tok cat="JJ">deficient</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="NN">CD45</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">Lck</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@19-12-2010
856295506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Further</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">normal</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CD45-</tok> <cons cat="ADJP" null="QSTN"></cons><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">Lck-deficient</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">Fas</tok> <tok cat="NN">stimulation</tok></cons> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Jun</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">JNK</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">proposed</tok> <tok cat="NN">mediator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">stress</tok> <tok cat="NN">activation</tok> <tok cat="NNS">pathways</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
856295507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Previous</tok> <tok cat="NNS">studies</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">endogenous</tok> <tok cat="NN">ceramide</tok> <tok cat="NN">release</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">Fas-mediated</tok> <tok cat="NN">apoptosis</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
856295508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">synthetic</tok> <tok cat="NN">ceramide</tok> <tok cat="NN">analog</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">JNK</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="COMP" null="ZERO"></cons><cons cat="NP" role="SBJ"><tok cat="NN">ceramide</tok> <tok cat="NN">release</tok></cons> <cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="ADJP"><tok cat="JJ">proximal</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">JNK</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Fas</tok></cons> <tok cat="NN">signaling</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
856295509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">although</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">CD45</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Lck</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Fas</tok></cons> <tok cat="NN">signaling</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">JNK</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="VP"><tok cat="VBG">transducing</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">distal</tok> <tok cat="NNS">signals</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">lead</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">apoptosis</tok></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Fas</tok></cons> <tok cat="NN">ligation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
856531701@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">Glucocorticoids</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">down-regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons> <tok cat="NN">mRNA</tok> <tok cat="NN">expression</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">asthma</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@11@@oe@19-12-2010
856531702@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="JJ">precise</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">action</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">remains</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">elucidated</tok><cons cat="NP" ref="i5" null="NONE"></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">glucocorticoids</tok></cons> <cons cat="VP"><tok cat="VBP">represent</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RBS">most</tok> <tok cat="JJ">effective</tok></cons> <tok cat="NN">therapy</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">asthma</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@19-12-2010
856531703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NNS">Interactions</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">AP-1</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">some</tok> <tok cat="NNS">genes</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons> <cons cat="NP"><tok cat="PRP">itself</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
856531704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">aim</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="S" role="PRD"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">compare</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">mRNA</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">blood</tok> <tok cat="NNS">monocytes</tok></cons> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">seven</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">unstable</tok></cons> <cons cat="ADJP"><tok cat="JJ">untreated</tok></cons></cons> <tok cat="JJ">asthmatic</tok> <tok cat="NNS">patients</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WP">who</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" id="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">subsequently</tok></cons> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">doses</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">parenteral</tok> <tok cat="NN">corticosteroid</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">methyl</tok> <tok cat="NN">prednisolone</tok> <cons cat="ADJP"><tok cat="CD">120</tok> <tok cat="NN">mg/day</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="CD">10</tok> <tok cat="NNS">days</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@19-12-2010
856531705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">mRNA</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">RNA</tok> <tok cat="NN">extraction</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="NN">RNAzol</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">analysed</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="JJ">reverse</tok> <tok cat="NN">transcriptase</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">polymerase</tok> <tok cat="NN">chain</tok> <tok cat="NN">reaction</tok></cons> <cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">semiquantitative</tok> <tok cat="JJ">competitive</tok> <tok cat="NN">hybridization</tok> <tok cat="NN">assay</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
856531706@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">All</tok> <tok cat="JJ">asthmatic</tok> <tok cat="NNS">patients</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">improvement</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">FEV1</tok> <tok cat="NNS">values</tok></cons></cons></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">corticosteroid</tok> <tok cat="NN">treatment</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="IN">per</tok> <tok cat="NN">cent</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">predicted</tok> <tok cat="NN">value</tok></cons></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">68.28</tok> <tok cat="CC">+/-</tok> <tok cat="CD">4.93</tok></cons> <tok cat="CC">versus</tok> <cons cat="NP"><tok cat="CD">95.57</tok> <tok cat="CC">+/-</tok> <tok cat="CD">6.41</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">P</tok></cons> <cons cat="ADJP"><tok cat="JJR">&lt;</tok> <cons cat="NP"><tok cat="CD">0.02</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">significant</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons> <cons cat="NP"><tok cat="NN">mRNA</tok></cons> <tok cat="NN">expression</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">P</tok></cons> <cons cat="ADJP"><tok cat="JJR">&lt;</tok> <cons cat="NP"><tok cat="CD">0.02</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@52@@oe@19-12-2010
856531707@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">first</tok> <tok cat="NN">report</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP"><tok cat="FW">ex</tok> <tok cat="FW">vivo</tok></cons> <tok cat="NN">down-regulation</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">glucocorticoid</tok> <tok cat="NN">receptor</tok></cons> <cons cat="NP"><tok cat="NN">mRNA</tok></cons> <tok cat="NN">expression</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">following</tok> <cons cat="NP"><tok cat="NN">corticosteroid</tok> <tok cat="NN">treatment</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
856695101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Translocation</tok> <tok cat="NNS">breakpoints</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">campomelic</tok> <tok cat="NN">dysplasia</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">autosomal</tok> <tok cat="NN">sex</tok> <tok cat="JJ">reversal</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="NN">map</tok> <cons cat="PP"><cons cat="NP"><cons cat="QP"><tok cat="JJR">more</tok> <tok cat="IN">than</tok> <tok cat="CD">130</tok></cons> <tok cat="NN">kb</tok></cons> <tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">SOX9</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@19-12-2010
856695102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" id="i5" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Campomelic</tok> <tok cat="NN">dysplasia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CMPD1</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">autosomal</tok> <tok cat="NN">XY</tok> <tok cat="NN">sex</tok> <tok cat="JJ">reversal</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SRA1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">caused</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">mutations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">SRY-related</tok> <tok cat="NN">gene</tok></cons> <cons cat="NP"><tok cat="NN">SOX9</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">17q</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@25@@oe@19-12-2010
856695103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Unexpectedly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">17q</tok> <tok cat="NNS">breakpoints</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">four</tok> <tok cat="NN">CMPD1</tok> <tok cat="NN">translocation</tok> <tok cat="NNS">cases</tok></cons> <cons cat="VP"><cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <tok cat="VBN">analyzed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="PRP">us</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">others</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">map</tok> <cons cat="PP"><cons cat="NP"><cons cat="NP"><tok cat="CD">50</tok> <tok cat="NN">kb</tok></cons> <cons cat="QP"><tok cat="CC">or</tok> <tok cat="JJR">more</tok></cons></cons> <tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">SOX9</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
856695104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">present</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">clinical</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">cytogenetic</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">molecular</tok></cons></cons> <tok cat="NNS">data</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">new</tok> <tok cat="NN">CMPD1/SRA1</tok> <tok cat="NN">patient</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">t(6;17)(q14;q24)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
856695105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Fluorescence</tok> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">situ</tok></cons> <tok cat="NN">hybridization</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">17q</tok> <tok cat="NN">breakpoint</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">case</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">maps</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NN">region</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">breakpoints</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">other</tok> <tok cat="NN">translocation</tok> <tok cat="NNS">cases</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="NP"><cons cat="QP"><tok cat="IN">at</tok> <tok cat="JJS">least</tok> <tok cat="CD">130</tok></cons> <tok cat="NN">kb</tok></cons> <tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">SOX9</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@19-12-2010
856695106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Likewise</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">breakpoints</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">previously</tok> <tok cat="VBN">described</tok></cons> <tok cat="NNS">cases</tok></cons></cons></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">map</tok> <cons cat="UCP" syn="COOD"><cons cat="NP" func="ADV"><cons cat="QP"><tok cat="JJR">more</tok> <tok cat="IN">than</tok> <tok cat="CD">130</tok></cons> <tok cat="NN">kb</tok></cons> <tok cat="CC">and</tok><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">pulsed</tok> <tok cat="NN">field</tok> <tok cat="NN">gel</tok> <tok cat="NN">electrophoresis</tok> <tok cat="NN">analysis</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="NP" syn="COOD"><tok cat="IN">at</tok> <tok cat="JJS">most</tok> <cons cat="NP"><tok cat="CD">400</tok> <tok cat="NN">kb</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="CD">690</tok> <tok cat="NN">kb</tok></cons></cons> <tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">SOX9</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@19-12-2010
856695107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">SOX9</tok> <tok cat="NN">coding</tok> <tok cat="NN">sequence</tok> <tok cat="NN">polymorphism</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">both</tok> <cons cat="NP"><tok cat="NN">SOX9</tok></cons> <tok cat="NNS">alleles</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">reverse</tok> <tok cat="NN">transcriptase</tok></cons> <tok cat="NN">polymerase</tok> <tok cat="NN">chain</tok> <tok cat="NN">reaction</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">lymphoblastoid</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">translocation</tok> <tok cat="NNS">cases</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
857021501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">TCL1</tok> <tok cat="NN">oncogene</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">transcripts</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">homologue</tok> <cons cat="NP"><tok cat="NN">MTCP1/c6.1B</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">leukaemic</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">non-leukaemic</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">ataxia</tok> <tok cat="NN">telangiectasia</tok> <tok cat="NNS">patients</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@25@@oe@19-12-2010
857021502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RB">recessively</tok> <tok cat="VBN">inherited</tok></cons> <tok cat="NN">disorder</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">ataxia</tok> <tok cat="NN">telangiectasia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">A-T</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">high</tok> <tok cat="NN">level</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">specific</tok> <cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="NNS">translocations</tok></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" id="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADVP"><tok cat="RB">easily</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VB">show</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">greatly</tok> <tok cat="VBN">increased</tok></cons> <tok cat="NN">predisposition</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">leukaemia/lymphoma</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">mainly</tok></cons> <tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok></cons> <tok cat="NN">origin</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@43@@oe@19-12-2010
857021503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">Some</tok> <cons cat="NP"><tok cat="NN">translocation</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">proliferate</tok> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">large</tok> <tok cat="NN">clone</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">develop</tok> <cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">prolymphocytic</tok> <tok cat="NN">leukaemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">T-PLL</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
857021504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">By</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">time</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">diagnosis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T-PLL</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="NN">clone</tok></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">many</tok> <tok cat="JJR">more</tok> <tok cat="JJ">genetic</tok></cons> <tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">additional</tok> <tok cat="NNS">translocations</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
857021505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">T-PLL</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">seen</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">non-A-T</tok> <tok cat="NNS">individuals</tok></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i7"><tok cat="WRB">where</tok></cons> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">TCL1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="NN">14q32</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">c6.1B/MTCP1</tok> <tok cat="NN">A1</tok> <tok cat="NN">transcript</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">at-Xq28</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="QP"><tok cat="RB">just</tok> <tok cat="DT">a</tok> <tok cat="JJ">few</tok></cons> <tok cat="NNS">instances</tok></cons></cons><cons cat="ADVP" ref="i7" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
857021506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TCL1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">leukaemic</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">A-T</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="CD">14</tok></cons> <tok cat="NNS">rearrangements</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
857021507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TCL1</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">occurs</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">preleukaemic</tok> <tok cat="NN">clone</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">A-T</tok> <tok cat="NNS">patients</tok></cons></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">primary</tok> <tok cat="NN">translocation</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
857021508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">Some</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TCL1</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">randomly</tok> <tok cat="VBN">selected</tok></cons> <tok cat="JJ">A-T</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP"><tok cat="JJ">large</tok> <tok cat="JJ">cytogenetic</tok> <tok cat="NNS">clones</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">any</tok> <tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">leukaemic</tok> <tok cat="NN">change</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
857021509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">B1</tok> <tok cat="NN">transcript</tok></cons></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">second</tok> <tok cat="NN">gene</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">MTCP1</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">occurred</tok> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">relatively</tok> <tok cat="JJ">high</tok></cons> <tok cat="NN">level</tok></cons></cons> <cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NN">T-PLL</tok> <tok cat="NNS">tumours</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">A-T</tok> <tok cat="NNS">patients</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">t(X;14)</tok> <tok cat="NNS">translocations</tok></cons></cons></cons></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">MTCP1/A1</tok> <tok cat="NN">transcript</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">much</tok> <tok cat="RBR">more</tok> <tok cat="RB">widely</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">tumour</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">non</tok> <tok cat="NN">tumour</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">A-T</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">non-A-T</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NNS">individuals</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@55@@oe@19-12-2010
857292501@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Aspirin-like</tok> <tok cat="NNS">drugs</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">protect</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="NN">benzoquinone</tok> <tok cat="NN">cytotoxicity</tok></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">NAD(P)H:quinone</tok> <tok cat="JJ">reductase-dependent</tok> <tok cat="NN">mechanism</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@19-12-2010
857292502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">Benzene</tok> <tok cat="NN">toxicity</tok></cons> <cons cat="PP"><tok cat="IN">towards</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">thought</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="NNS">metabolites</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">benzene</tok></cons></cons></cons> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">benzoquinone</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">BQ</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@19-12-2010
857292503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">NAD(P)H:quinone</tok> <tok cat="NN">reductase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">QR</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">protect</tok> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="NN">BQ</tok> <tok cat="NN">toxicity</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@19-12-2010
857292504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">QR</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
857292505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">aspirin-like</tok> <tok cat="NNS">drugs</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ALD</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBP">induce</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
857292506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">It</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons> <cons cat="VP"><tok cat="VBN">hypothesized</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="NN">ALD</tok></cons> <cons cat="VP"><tok cat="MD">would</tok> <cons cat="VP"><tok cat="VB">protect</tok> <cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="NN">BQ</tok> <tok cat="NN">toxicity</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inducing</tok> <cons cat="NP"><tok cat="NN">QR</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
857292507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Molt-4</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">M4</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">incubated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NNS">concentrations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NN">ALD</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">flurbiprofen</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">sodium</tok> <tok cat="NN">diclofenac</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">then</tok></cons> <cons cat="VP"><tok cat="VBN">exposed</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">BQ</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
857292508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="NN">Toxicity</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">measured</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">viability</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">trypan</tok> <tok cat="JJ">blue</tok> <tok cat="NN">exclusion</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@19-12-2010
857292509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">Both</tok> <tok cat="NNS">drugs</tok></cons> <cons cat="VP"><tok cat="VBD">protected</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="NN">BQ</tok> <tok cat="NN">cytotoxicity</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">dose-dependent</tok> <tok cat="NN">manner</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="FW">e.g.</tok></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">sodium</tok> <tok cat="NN">diclofenac</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="CD">15</tok> <tok cat="NN">microM</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">reduced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">fraction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">BQ-treated</tok> <tok cat="JJ">dead</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="CD">70</tok><tok cat="NN">%</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
857292510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NNS">ALDs</tok></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">QR</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">M4</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NN">range</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">concentrations</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i12"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i12" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">protected</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="NN">BQ</tok> <tok cat="NN">toxicity</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
857292511@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i13" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">protective</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ALD</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP"><tok cat="VBN">reduced</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">dicoumarol</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">QR-specific</tok> <tok cat="NN">inhibitor</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
857292512@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">Since</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons> <cons cat="VP"><tok cat="VBP">do</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">metabolize</tok> <cons cat="NP"><tok cat="JJ">arachidonic</tok> <tok cat="NN">acid</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">our</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">ALD</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">protect</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">metabolite</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">benzene</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">QR</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@19-12-2010
857338101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="JJ">Human</tok> <tok cat="NN">herpesvirus</tok> <tok cat="CD">6</tok> <cons cat="NP"><tok cat="NN">variant</tok> <tok cat="NN">A</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">variant</tok> <tok cat="NN">B</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">infects</tok> <cons cat="NP"><tok cat="JJ">EBV-positive</tok> <tok cat="NN">B</tok> <tok cat="JJ">lymphoid</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">activating</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">latent</tok> <tok cat="NN">EBV</tok> <tok cat="NN">genome</tok></cons></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">BZLF-1-dependent</tok> <tok cat="NN">mechanism</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@19-12-2010
857338102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="JJ">Human</tok> <tok cat="NN">herpesvirus</tok> <tok cat="CD">6</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">predominantly</tok> <tok cat="NN">T</tok> <tok cat="JJ">lymphotropic</tok></cons> <tok cat="NN">virus</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">infect</tok> <cons cat="NP"><tok cat="DT">some</tok> <cons cat="NP"><tok cat="JJ">EBV</tok><tok cat="JJ">-positive</tok> <tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">induce</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="PRP">them</tok></cons></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">EBV</tok> <tok cat="JJ">lytic</tok> <tok cat="NN">cycle</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@36@@oe@19-12-2010
857338103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">confirmed</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">extended</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="JJ">such</tok> <tok cat="NNS">observations</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">showing</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">1</tok><tok cat="RRB">)</tok></cons> <cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">phenomenon</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">restricted</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">variant</tok> <tok cat="NN">A</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HHV-6</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">fact</tok></cons></cons> <cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">isolates</tok></cons> <cons cat="VP"><tok cat="VBG">belonging</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">HHV-6</tok> <tok cat="NN">variant</tok> <tok cat="NN">B</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">BA92</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Z29</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="CC">neither</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="TO">to</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VB">infect</tok> <cons cat="NP"><tok cat="DT">any</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><cons cat="ADVP"><tok cat="RB">independently</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">EBV</tok> <tok cat="NN">status</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">nor</tok> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VB">induce</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">EBV</tok> <tok cat="NN">genome</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@64@@oe@19-12-2010
857338104@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">only</tok> <tok cat="NN">exception</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">represented</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">P3HR1</tok> <tok cat="NNS">cells</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="NN">variant</tok> <tok cat="NN">B</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">EBV</tok> <tok cat="NNS">antigens</tok></cons></cons></cons><cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">2</tok><tok cat="RRB">)</tok></cons> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">EBV</tok> <tok cat="NN">genome</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">contributes</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">susceptibility</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">HHV-6</tok> <tok cat="NN">infection</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">increasing</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBG">binding</tok> <tok cat="NNS">sites</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">percentage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">infectable</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">immunoelectron</tok> <tok cat="NN">microscopy</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="LST"><tok cat="LRB">(</tok><tok cat="LS">3</tok><tok cat="RRB">)</tok></cons> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">HHV-6</tok> <tok cat="JJ">infected</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBN">transfected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">plasmids</tok></cons> <cons cat="VP"><tok cat="VBG">bearing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NNS">regions</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">EBV</tok> <tok cat="RB">early</tok> <tok cat="NNS">genes</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">BZLF1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">BMRF1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">strong</tok> <tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NNS">promoters</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@101@@oe@19-12-2010
857611101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Identification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">physical</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">calcineurin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="IN">of</tok> <tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NFATp</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@19-12-2010
857611102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">calcium/calmodulin-dependent</tok> <tok cat="JJ">serine/threonine</tok> <tok cat="NN">phosphatase</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">calcineurin</tok></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">pivotal</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">transducing</tok> <cons cat="NP"><tok cat="JJ">membrane-associated</tok> <tok cat="NNS">signals</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@19-12-2010
857611103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="CD">One</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">putative</tok> <tok cat="NNS">targets</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">calcineurin</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">pre-existing</tok><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">cytosolic</tok> <tok cat="NN">component</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="IN">of</tok> <tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP" id="i5"><tok cat="NN">NFATp</tok></cons><tok cat="COLON">;</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">referred</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" ref="i5" null="NONE"></cons></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="NN">NFAT1</tok></cons></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="CD">2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@19-12-2010
857611104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">calcineurin</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immunosuppressive</tok> <tok cat="NNS">drugs</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">cyclosporin</tok> <tok cat="NN">A</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">FK506</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">prevents</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">dephosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFATp</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
857611105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">physical</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">calcineurin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NFATp</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok><cons cat="NP" ref="i8" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
857611106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NFATp</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">lysates</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="VBN">immobilized</tok> <cons cat="NP"><tok cat="NN">calcineurin</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
857611107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">calcium</tok> <tok cat="NN">ionophore</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">shift</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">weight</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFATp</tok></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">due</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">dephosphorylation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
857611108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">dephosphorylation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">cyclosporin</tok> <tok cat="NN">A</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">FK506</tok></cons></cons></cons> <cons cat="ADJP" adv="TMP"><tok cat="JJ">prior</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
857611109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Of</tok> <cons cat="NP"><tok cat="NN">note</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">phosphorylated</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">dephosphorylated</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">form</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFATp</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">bound</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">calcineurin</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
857611110@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">both</tok> <tok cat="NNS">forms</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NFATp</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">calcineurin</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">pretreatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">calcineurin</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">complex</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">FK506</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">ligand</tok> <tok cat="NN">FKBP12</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
857611111@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">Taken</tok> <cons cat="NP" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">together</tok></cons></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">data</tok></cons> <cons cat="ADVP"><tok cat="RB">strongly</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="UCP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">direct</tok> <tok cat="NN">interaction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">calcineurin</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">NFATp</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">interaction</tok></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">depend</tok> <cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">phosphorylation</tok> <tok cat="NNS">sites</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NFATp</tok></cons> <cons cat="VP"><tok cat="VBN">affected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">activation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
857974801@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">BSAP</tok></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">key</tok> <tok cat="NN">regulator</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">B-cell</tok></cons> <cons cat="NP"><tok cat="NN">development</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@11@@oe@19-12-2010
857974802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">B-cell-specific</tok> <tok cat="NN">activator</tok> <tok cat="NN">protein</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">BSAP</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">recently</tok> <tok cat="VBN">identified</tok></cons> <tok cat="NN">member</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Pax-gene</tok> <tok cat="NN">family</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">lymphoid</tok> <tok cat="NN">system</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><tok cat="NN">BSAP</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@32@@oe@19-12-2010
857974803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="NNP">Markus</tok> <tok cat="NNP">Neurath</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Eckhard</tok> <tok cat="NNP">Stuber</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNP">Warren</tok> <tok cat="NNP">Strober</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">describe</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">structure</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">BSAP</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">focus</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">regulate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">B-cell-specific</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
857974804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">They</tok></cons> <cons cat="VP"><tok cat="VBP">propose</tok> <cons cat="SBAR" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">BSAP</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">key</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP" syn="COOD"><cons cat="CONJP"><tok cat="RB">not</tok> <tok cat="RB">only</tok></cons> <cons cat="VP"><tok cat="VBP">influence</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">development</tok></cons></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">influences</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">balance</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">proliferation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">immunoglobulin</tok></cons> <tok cat="NN">secretion</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJR">later</tok> <tok cat="NNS">stages</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">B-cell</tok> <tok cat="NN">differentiation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@19-12-2010
858037801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">JAK3</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">JAK1</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">critical</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">IL-2-induced</tok> <tok cat="NN">proliferation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">STAT5</tok></cons> <tok cat="NN">recruitment</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">COOH-terminal</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">receptor</tok> <tok cat="NN">beta-chain</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@26@@oe@19-12-2010
858037802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i4" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">cytokines</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">growth</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">use</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">JAK-STAT</tok> <tok cat="NN">pathway</tok></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">signal</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cell</tok> <tok cat="NN">membrane</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nucleus</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@19-12-2010
858037803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">While</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="VBG">homodimerizing</tok> <tok cat="NN">cytokine</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">transmit</tok> <cons cat="NP"><tok cat="NN">signal</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">single</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">JAK</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="PP"><tok cat="FW">i.e.</tok></cons> <cons cat="NP"><tok cat="NN">growth</tok> <tok cat="NN">hormone</tok> <tok cat="NNS">receptors</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="JJ">several</tok> <cons cat="NP"><tok cat="JJ">multicomponent</tok> <tok cat="NN">cytokine</tok> <tok cat="NNS">receptors</tok></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">require</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">simultaneous</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">pairs</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <cons cat="NP"><tok cat="NN">JAK</tok> <tok cat="NNS">kinases</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="PP"><tok cat="FW">i.e.</tok></cons> <cons cat="NP"><tok cat="NN">interferon</tok> <tok cat="NNS">receptors</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@19-12-2010
858037804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Recent</tok> <tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">preferential</tok> <tok cat="NN">coupling</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" id="i40"><tok cat="NN">JAK3</tok></cons></cons> <cons cat="PP" id="i41"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin-2</tok> <tok cat="NN">receptor-gamma</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">gamma</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><cons map="i40" cat="NP"><tok cat="NN">JAK1</tok></cons> <cons map="i41" cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">beta</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">supports</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">concept</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">heterotrans-activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">JAK1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">JAK3</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">caused</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">IL-2-induced</tok> <tok cat="NN">heterodimerization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">receptor</tok> <tok cat="NNS">partners</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@19-12-2010
858037805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <tok cat="JJ">present</tok> <tok cat="NN">study</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">verified</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">cause</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">JAK1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">JAK3</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i7" role="SBJ"><tok cat="NN">IL-2</tok></cons> <cons cat="VP"><tok cat="VBD">stimulated</tok> <cons cat="NP"><tok cat="NN">JAK3</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">significantly</tok> <tok cat="JJR">larger</tok></cons> <tok cat="NN">extent</tok></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">than</tok> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><cons cat="VP" null="QSTN"></cons><cons cat="NP"><cons cat="NP"><tok cat="NN">JAK1</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">YT</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@19-12-2010
858037806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">conclusion</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">based</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="JJ">independent</tok> <tok cat="NNS">criteria</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RBR">more</tok> <tok cat="JJ">vigorous</tok></cons> <tok cat="NN">tyrosine</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">JAK3</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RBR">more</tok> <tok cat="JJ">marked</tok> <tok cat="JJ">enzymatic</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">JAK3</tok></cons></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJR">higher</tok> <tok cat="NN">abundance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">JAK3</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">receptor</tok> <tok cat="NNS">complexes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@19-12-2010
858037807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i9"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="JJ">human</tok> <tok cat="NN">IL-2R</tok> <tok cat="NN">beta</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">stably</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">BA/F3</tok> <tok cat="NNS">cells</tok></cons></cons><cons cat="ADVP" ref="i9" null="T"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="ADJP"><tok cat="JJ">robust</tok></cons> <cons cat="NP"><tok cat="JJ">IL-2-induced</tok> <tok cat="NN">proliferation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">JAK3</tok></cons> <tok cat="NN">activation</tok></cons></cons> <cons cat="VP"><tok cat="VBD">occurred</tok> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">detectable</tok> <tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="NN">JAK1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">JAK2</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">TYK2</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
858037808@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons> <cons cat="VP"><tok cat="VBP">propose</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">receptor</tok></cons> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">depend</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">equimolar</tok> <tok cat="NN">heterodimerization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">JAK1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">JAK3</tok></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="VBG">following</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">IL-2-induced</tok> <tok cat="NN">heterodimerization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">beta</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">gamma</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
858037809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Nonetheless</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">membrane-proximal</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">beta</tok></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Asn240-Leu335</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">critical</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">JAK3</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">amount</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">JAK3</tok></cons> <cons cat="ADJP"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">receptor</tok> <tok cat="NNS">complexes</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">increased</tok> <cons cat="PP" adv="TMP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">time</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">stabilization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">JAK3</tok></cons> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">receptor</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">relies</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">beta</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">gamma</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@52@@oe@19-12-2010
858037810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Moreover</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i11" role="SBJ"><tok cat="NN">STAT5</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">predominant</tok> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="VP"><tok cat="VBD">required</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">COOH-terminal</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">beta</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Ser386-Val525</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="NN">STAT5</tok></cons> <tok cat="NN">recruitment</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">correlated</tok> <cons cat="NP" ref="i12" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-2R</tok> <tok cat="NN">gamma</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">JAK3</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@19-12-2010
859383101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">vitamin</tok> <tok cat="NN">D</tok> <tok cat="JJ">receptor-retinoid</tok> <tok cat="NN">X</tok> <tok cat="JJ">receptor-vitamin</tok> <tok cat="NN">D</tok> <tok cat="NN">response</tok> <tok cat="NN">element</tok> <tok cat="JJ">complex</tok></cons> <tok cat="NN">formation</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">vitamin</tok> <tok cat="JJ">D-resistant</tok> <tok cat="NN">New</tok> <tok cat="NN">World</tok> <tok cat="JJ">primate</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@19-12-2010
859383102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJS">Most</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">New</tok> <tok cat="NN">World</tok> <tok cat="NN">primate</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NWP</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">genera</tok></cons> <cons cat="VP"><tok cat="VBD">evolved</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">require</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="JJ">circulating</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">steroid</tok> <tok cat="NNS">hormones</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">vitamin</tok> <tok cat="NN">D</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@19-12-2010
859383103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">hypothesized</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">an</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">intracellular</tok> <tok cat="NN">vitamin</tok> <tok cat="NN">D</tok> <tok cat="NN">binding</tok> <tok cat="NN">protein</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IDBP</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">nuclear</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">cytoplasmic</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">fractions</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NWP</tok> <tok cat="NNS">cells</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="DT">another</tok> <tok cat="NN">protein</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">cause</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="VP"><tok cat="VB">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons></cons><cons cat="NP" id="i21"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">steroid</tok> <tok cat="JJ">hormone-resistant</tok> <tok cat="NN">state</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">NWP</tok></cons></cons></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">disruption</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">receptor</tok> <tok cat="NN">dimerization</tok> <tok cat="NN">process</tok></cons></cons></cons></cons> <tok cat="CC">and/or</tok> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">interference</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">receptor</tok> <tok cat="NN">complex</tok></cons> <cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">consensus</tok> <tok cat="NN">response</tok> <tok cat="NNS">elements</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">enhancer</tok> <tok cat="NNS">regions</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">steroid-responsive</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@69@@oe@19-12-2010
859383104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">employed</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">electromobility</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">EMSA</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">screen</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">proteins</tok></cons> <cons cat="ADJP"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">vitamin</tok> <tok cat="NN">D</tok> <tok cat="NN">response</tok> <tok cat="NN">element</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">VDRE</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
859383105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i8" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">Nuclear</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">post-nuclear</tok></cons> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NNS">extracts</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">prepared</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP" id="i9"><tok cat="CD">two</tok> <cons cat="NP"><tok cat="JJ">B-lymphoblastoid</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons> <cons cat="VP"><tok cat="VBN">known</tok> <cons cat="NP" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">representative</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">vitamin</tok> <tok cat="JJ">D-resistant</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">wild</tok> <tok cat="NN">type</tok> <tok cat="NNS">phenotypes</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
859383106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="DT">The</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">compared</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="PRPP">their</tok> <tok cat="NN">ability</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">retard</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">migration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="VBN">radiolabeled</tok></cons> <cons cat="ADJP"><tok cat="JJ">double</tok> <tok cat="JJ">stranded</tok></cons></cons> <tok cat="NNS">oligomers</tok> <tok cat="NN">representative</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NNS">VDREs</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">osteocalcin</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mouse</tok> <tok cat="NN">osteopontin</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NN">gene</tok> <tok cat="NNS">promoters</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
859383107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i12" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">specific</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">retarded</tok></cons></cons> <tok cat="NN">band</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="NN">VDR-RXR</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" ref="i12" null="NONE"></cons></cons><cons cat="SBAR" syn="COOD" func="ADV" adv="TMP"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i13"><tok cat="WRB">when</tok></cons> <cons cat="FRAG"><cons cat="NP" role="SBJ"><tok cat="JJ">wild</tok> <tok cat="NN">type</tok> <tok cat="NN">cell</tok></cons></cons></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i14"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i15" role="SBJ"><tok cat="NN">vitamin</tok> <tok cat="JJ">D-resistant</tok> <tok cat="NN">cell</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">extract</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok> <tok cat="NN">reaction</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">either</tok> <tok cat="NN">probe</tok></cons></cons></cons></cons><cons cat="ADVP" ref="i14" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
859383108@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">vitamin</tok> <tok cat="JJ">D-resistant</tok> <tok cat="NN">cell</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">extract</tok></cons> <cons cat="VP"><tok cat="VBD">contained</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">protein</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i16"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i16" id="i18" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">bound</tok> <cons cat="NP" ref="i18" null="NONE"></cons><cons cat="PP"><cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">VDRE</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i18" role="SBJ" null="NONE"></cons><cons cat="VP"><cons cat="ADVP"><tok cat="RB">completely</tok></cons> <tok cat="VBG">inhibiting</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">VDR-RXR-VDRE</tok> <tok cat="NN">complex</tok></cons> <tok cat="NN">formation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok> <cons cat="S"><cons cat="NP" id="i19" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">effects</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">demonstrated</tok> <cons cat="NP" ref="i19" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">extract</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">wild</tok> <tok cat="NN">type</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">post-nuclear</tok> <tok cat="NN">extract</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">vitamin</tok> <tok cat="JJ">D-resistant</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@57@@oe@19-12-2010
859383109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">conclude</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i2" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">VDRE-binding</tok> <tok cat="NN">protein</tok></cons><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><cons cat="ADJP"><tok cat="JJ">distinct</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">IDBP</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">extract</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">prototypical</tok> <tok cat="NN">vitamin</tok> <tok cat="JJ">D-resistant</tok> <tok cat="NN">NWP</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i2" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">inhibiting</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">normal</tok> <cons cat="NP"><tok cat="NN">VDR-RXR</tok></cons> <tok cat="NN">heterodimer</tok></cons> <cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">VDRE</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@19-12-2010
859601701@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Characterization</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">purification</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok> <tok cat="NN">substrate</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
859601702@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Identification</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">lymphocyte-specific</tok> <tok cat="NN">protein</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LSP1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@9@@oe@19-12-2010
859601703@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Incubation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">B-chronic</tok> <tok cat="JJ">lymphocytic</tok> <tok cat="NN">leukemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">B-CLL</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NNS">esters</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="JJ">major</tok> <tok cat="NN">PKC</tok> <tok cat="NNS">substrates</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">MARCKS</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="VBN">myristoylated</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADJP"><tok cat="JJ">alanine-rich</tok></cons></cons> <tok cat="NN">C</tok> <tok cat="NN">kinase</tok> <tok cat="NN">substrate</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">MRP</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">MARCKS-related</tok> <tok cat="NN">protein</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">third</tok> <tok cat="NN">protein</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">apparent</tok> <tok cat="NN">m.w.</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">60,000</tok></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i69"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i69" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RBS">most</tok> <tok cat="JJ">prominent</tok></cons> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok> <tok cat="NN">substrate</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@63@@oe@19-12-2010
859601704@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i70" role="SBJ"><tok cat="NN">p60</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">time</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">PMA</tok> <tok cat="NN">dose</tok></cons></cons> <tok cat="JJ">dependent</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i70" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">cell-permeable</tok> <tok cat="NN">diacylglycerol</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">inactive</tok> <tok cat="NN">phorbol</tok> <tok cat="NNS">esters</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
859601705@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Two-dimensional</tok> <tok cat="JJ">electrophoretic</tok> <tok cat="NN">analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">protein</tok> <tok cat="NN">phosphorylation</tok> <tok cat="NN">pattern</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">CESS</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">identity</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">p60</tok> <tok cat="NN">protein</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">that</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B-CLL</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
859601706@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i71" role="SBJ"><tok cat="NN">p60</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">purified</tok> <cons cat="NP" ref="i71" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="NN">CESS</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">peptide</tok> <tok cat="NN">microsequencing</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">revealed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S" misc="CLF"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="JJ">lymphocyte-specific</tok> <tok cat="NN">protein</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LSP1</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i72"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i72" id="i73" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="VP"><tok cat="VBN">characterized</tok> <cons cat="NP" ref="i73" null="NONE"></cons><cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="RBS">most</tok> <tok cat="JJ">prominent</tok></cons> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok> <tok cat="JJ">substrate</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@19-12-2010
859821001@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Cross-linking</tok> <cons cat="NP"><tok cat="NN">CD40</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">preferentially</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">stress-activated</tok> <tok cat="NN">protein</tok> <tok cat="NNS">kinases</tok></cons> <cons cat="CONJP"><tok cat="RB">rather</tok> <tok cat="IN">than</tok></cons> <cons cat="NP"><tok cat="JJ">mitogen-activated</tok> <tok cat="NN">protein</tok> <tok cat="NNS">kinases</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@19-12-2010
859821002@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="VBD">cell-associated</tok> <tok cat="NN">surface</tok> <tok cat="NN">molecule</tok></cons> <cons cat="NP"><tok cat="NN">CD40</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">key</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="JJ">cell-dependent</tok> <tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">maturation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">individuals</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">defects</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="NN">CD40</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">ligand</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">impaired</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">immunoglobulin</tok></cons> <tok cat="NN">isotype</tok> <tok cat="NN">class</tok> <tok cat="NN">switching</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">germinal</tok> <tok cat="NN">center</tok> <tok cat="NN">formation</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@39@@oe@19-12-2010
859821003@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">CD40</tok></cons> <tok cat="NN">signaling</tok></cons> <cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">downstream</tok> <tok cat="NNS">effectors</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Lyn</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phosphatidylinositol-3-kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PI-3</tok> <tok cat="NN">kinase</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">NF-kappa</tok> <tok cat="NN">B</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
859821004@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="JJ">stress-activated</tok> <tok cat="NN">protein</tok> <tok cat="NNS">kinases</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">SAPK</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">CD40</tok></cons> <tok cat="NN">cross-linking</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">various</tok></cons> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">tonsillar</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
859821005@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">The</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" syn="COOD" role="PRD"><cons cat="ADJP"><tok cat="JJ">rapid</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">transient</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cyclosporin</tok> <tok cat="JJ">A-insensitive</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
859821006@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i7" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="NONE"></cons><tok cat="RB">not</tok> <cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">rely</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">C</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
859821007@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">While</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">CD40</tok></cons> <tok cat="NN">ligation</tok></cons> <cons cat="ADVP"><tok cat="RB">strongly</tok> <cons cat="PRN" ref="i33" null="ICH"></cons></cons><cons cat="VP"><tok cat="VBZ">activates</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NNS">SAPKs</tok></cons></cons> <cons cat="PRN" id="i33"><tok cat="LRB">(</tok><cons cat="NP" func="ADV"><tok cat="RB">up</tok> <tok cat="TO">to</tok> <tok cat="JJ">25-fold</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">does</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">affect</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">mitogen-activated</tok> <tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">family</tok></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">MAPK</tok></cons><tok cat="COLON">;</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ERK1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">ERK2</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
859821008@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="JJ">Consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">data</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">CD40</tok></cons> <tok cat="NNS">signals</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">up-regulate</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-jun</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">c-fos</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">mRNA</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">alter</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ATF2</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Raf-1</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
859821009@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">summary</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">CD40</tok></cons> <tok cat="NN">signaling</tok></cons> <cons cat="ADVP"><tok cat="RB">preferentially</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">SAPK</tok></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">MAPK</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@12@@oe@19-12-2010
859848101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Dehydroepiandrosterone</tok> <tok cat="NN">modulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">lipopolysaccharide-stimulated</tok> <tok cat="NN">monocyte</tok> <tok cat="NN">cytotoxicity</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@7@@oe@19-12-2010
859848102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">Dehydroepiandrosterone</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">DHEA</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">predominant</tok> <tok cat="NN">androgen</tok></cons> <cons cat="VP"><tok cat="VBN">secreted</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="JJ">adrenal</tok> <tok cat="NN">cortex</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">converted</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADJP"><tok cat="JJ">potent</tok></cons> <cons cat="NP"><tok cat="NNS">androgens</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">estrogens</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@19-12-2010
859848103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">precursor</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NN">steroid</tok> <tok cat="NNS">hormones</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><tok cat="NN">DHEA</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">proposed</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">immunity</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
859848104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">study</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">investigated</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">DHEA</tok> <tok cat="NN">modulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">LPS-induced</tok> <tok cat="NN">monocyte</tok> <tok cat="NN">cytotoxicity</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@11@@oe@19-12-2010
859848105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Cytotoxicity</tok> <tok cat="NNS">markers</tok></cons> <cons cat="VP"><tok cat="VBN">assessed</tok> <cons cat="NP" null="NONE"></cons></cons></cons><cons cat="VP"><tok cat="VBP">include</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">cell</tok> <tok cat="NN">killing</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-1</tok></cons> <tok cat="NN">secretion</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">reactive</tok> <tok cat="NN">oxygen</tok> <tok cat="JJ">intermediate</tok> <tok cat="NN">release</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nitric</tok> <tok cat="NN">oxide</tok> <tok cat="NN">synthetase</tok></cons> <tok cat="NN">activity</tok></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">measured</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">release</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">reactive</tok> <tok cat="NN">nitrogen</tok> <tok cat="NNS">intermediates</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">complement</tok> <tok cat="NN">receptor-1</tok> <tok cat="NN">cell</tok> <tok cat="NN">surface</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">protein</tok></cons> <tok cat="NN">presence</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@19-12-2010
859848106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Monocytes</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">LPS</tok> <tok cat="NNS">concentrations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">1.0</tok> <tok cat="NNS">micrograms/ml</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">displayed</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">above</tok> <tok cat="JJ">cytotoxic</tok> <tok cat="NNS">markers</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">monocytes</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">DHEA</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">LPS</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJR">lower</tok> <tok cat="NN">concentration</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">0.2</tok> <tok cat="NN">ng/ml</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok><cons cat="VP" null="QSTN"></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
859848107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i7"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">simultaneously</tok></cons><cons cat="ADVP" ref="i7" null="T"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" syn="COOD" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">DHEA</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">LPS</tok></cons> <cons cat="ADJP"><tok cat="CD">0.2</tok> <tok cat="NN">ng/ml</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">displayed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">synergistic</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">monocyte</tok> <tok cat="NN">cytotoxicity</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">cancerous</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-1</tok></cons> <tok cat="NN">secretion</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">reactive</tok> <tok cat="NN">nitrogen</tok> <tok cat="JJ">intermediate</tok> <tok cat="NN">release</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">complement</tok> <tok cat="NN">receptor-1</tok> <tok cat="NN">cell-surface</tok> <tok cat="NN">protein</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">TNF-alpha</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">levels</tok></cons> <cons cat="ADJP"><tok cat="JJ">comparable</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">levels</tok></cons> <cons cat="VP"><tok cat="VBN">obtained</tok> <cons cat="NP" null="NONE"></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">LPS</tok></cons> <cons cat="ADJP"><tok cat="CD">1.0</tok> <tok cat="NN">microgram/ml</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@50@@oe@19-12-2010
859848108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">Scatchard</tok> <tok cat="NN">plot</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">DHEA</tok> <tok cat="NN">receptor</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="NN">DHEA</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">LPS-stimulated</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">receptor-dependent</tok> <tok cat="NN">process</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
860304501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">involvement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p23</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">hsp90</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">immunophilins</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">assembly</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">progesterone</tok> <tok cat="NN">receptor</tok> <tok cat="NNS">complexes</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@17@@oe@19-12-2010
860304502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="ADVP"><tok cat="RBR">better</tok></cons> <cons cat="VP"><tok cat="VB">understand</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">assembly</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">progesterone</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PR</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">developed</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cell-free</tok> <tok cat="NNS">systems</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">studying</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">interactions</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PR</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">hsp90</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">other</tok> <cons cat="NP"><tok cat="VBN">associated</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@32@@oe@19-12-2010
860304503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i5"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">PR</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">incubated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">rabbit</tok> <tok cat="NN">reticulocyte</tok> <tok cat="NN">lysate</tok></cons></cons><cons cat="ADVP" ref="i5" null="T"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">association</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">hsp90</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">hsp70</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">three</tok> <cons cat="NP"><tok cat="JJ">immunophilins</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">FKBP54</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">FKBP52</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CyP-40</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">p23</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">observed</tok><cons cat="NP" ref="i8" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
860304504@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">interactions</tok></cons> <cons cat="VP"><tok cat="VBP">require</tok> <cons cat="NP"><tok cat="NN">ATP/Mg2+</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i9"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">ATP</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBG">limiting</tok> <cons cat="ADVP" ref="i9" null="T"></cons></cons></cons></cons></cons><cons cat="S"><cons cat="NP" id="i10" role="SBJ"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">PR</tok></cons> <tok cat="NN">complex</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">altered</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p60</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p48</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBG">lacking</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">immunophilins</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p23</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@29@@oe@19-12-2010
860304505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">studied</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <cons cat="NP"><tok cat="JJ">pre-formed</tok> <tok cat="NN">hsp90</tok> <tok cat="NNS">complexes</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i11"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">participate</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">assembly</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PR</tok> <tok cat="NNS">complexes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
860304506@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="CD">One</tok></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">hsp90</tok></cons> <cons cat="VP"><tok cat="VBN">bound</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">hsp70</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p60</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">complex</tok></cons> <cons cat="VP"><tok cat="VBZ">forms</tok> <cons cat="ADVP"><tok cat="RB">spontaneously</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">absence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ATP</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
860304507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">A</tok> <tok cat="JJ">second</tok> <tok cat="NN">complex</tok></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">hsp90</tok></cons> <cons cat="VP"><tok cat="VBN">bound</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">p23</tok></cons> <tok cat="CC">plus</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="CD">three</tok> <cons cat="NP"><tok cat="NNS">immunophilins</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">some</tok> <cons cat="NP"><tok cat="NN">hsp70</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
860304508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">formation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">complex</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP"><tok cat="NN">ATP</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@8@@oe@19-12-2010
860304509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">further</tok> <tok cat="NNS">studies</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="UCP" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="VBN">purified</tok> <cons cat="NP"><tok cat="NN">hsp90</tok></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="VBN">purified</tok> <cons cat="NP"><tok cat="NN">p23</tok></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="COMP" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">interaction</tok></cons> <cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">ATP</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">molybdate</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
860304510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBZ">explains</tok><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">part</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">known</tok> <tok cat="NNS">effects</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ATP</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">molybdate</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">assembly</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PR</tok> <tok cat="NNS">complexes</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
860594101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Autocrine</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">interferon-gamma</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="PP"><tok cat="VBG">following</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">activation</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@12@@oe@19-12-2010
860594102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">engagement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">receptor/CD3</tok> <tok cat="NN">complex</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">co-stimulatory</tok> <tok cat="NNS">molecules</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">triggering</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">lead</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="LRB">(</tok><tok cat="NN">NF</tok><tok cat="RRB">)</tok><tok cat="NN">-kappa</tok> <tok cat="NN">B</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@50@@oe@19-12-2010
860594103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="NN">result</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">activation</tok> <tok cat="NN">process</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">genes</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="VP"><tok cat="VBG">encoding</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cytokines</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">interleukin-2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">tumor</tok> <tok cat="NN">necrosis</tok> <tok cat="NN">factor-alpha</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">interferon</tok> <tok cat="LRB">(</tok><tok cat="NN">IFN</tok><tok cat="RRB">)</tok><tok cat="NN">-gamma</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">have</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">important</tok></cons> <cons cat="ADJP"><cons cat="NP"><tok cat="JJ">immunoregulatory</tok></cons></cons></cons> <tok cat="NNS">effects</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@19-12-2010
860594104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i33" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">DNA-binding</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="NN">STAT1</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBZ">becomes</tok> <cons cat="VP"><tok cat="JJ">activated</tok> <cons cat="NP" ref="i33" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">although</tok> <cons cat="FRAG"><tok cat="RB">not</tok> <cons cat="PP" adv="TMP"><tok cat="IN">until</tok> <cons cat="NP"><cons cat="NP"><cons cat="QP"><tok cat="CD">1-2</tok></cons> <tok cat="NN">h</tok></cons> <cons cat="PP" adv="TMP"><tok cat="IN">after</tok> <cons cat="NP"><tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
860594105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="NN">Activation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">delayed</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="NP" func="ADV"><tok cat="DT">a</tok> <tok cat="JJ">further</tok> <cons cat="QP"><tok cat="CD">1-2</tok></cons> <tok cat="NN">hr</tok></cons></cons> <cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i7"><tok cat="WRB">when</tok></cons> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="JJ">mononuclear</tok> <tok cat="NN">cell</tok> <tok cat="NNS">cultures</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">antigen</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">requires</tok> <cons cat="NP"><tok cat="NN">processing</tok></cons></cons></cons></cons></cons></cons><cons cat="ADVP" ref="i7" null="T"></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
860594106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="NN">Appearance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">STAT1</tok></cons> <tok cat="NN">factor</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">reduced</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cyclosporin</tok> <tok cat="NN">A</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">blocked</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">cycloheximide</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">indicating</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">its</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">protein</tok><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons> <cons cat="VP"><tok cat="VBN">synthesized</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">initial</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">events</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@40@@oe@19-12-2010
860594107@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="S" func="NOM" role="SBJ"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Neutralizing</tok> <cons cat="NP"><tok cat="NN">antiserum</tok></cons> <cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">other</tok> <cons cat="NP"><tok cat="NNS">cytokines</tok></cons></cons> <cons cat="VP"><tok cat="VBN">tested</tok><cons cat="NP" null="NONE"></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">blocked</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">factor</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">almost</tok> <tok cat="RB">completely</tok></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="NN">IFN-gamma</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">culture</tok> <tok cat="NNS">supernatants</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">stimulated</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">levels</tok></cons> <cons cat="SBAR"><cons cat="WHPP" id="i12"><tok cat="IN">at</tok> <cons cat="WHNP"><tok cat="WDT">which</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">recombinant</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">naive</tok> <tok cat="NNS">cells</tok></cons></cons></cons><cons cat="PP" ref="i12" null="T"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@44@@oe@19-12-2010
860594108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i13" role="SBJ"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">STAT1</tok></cons> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i13" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">synthesized</tok></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">released</tok></cons></cons> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NN">lymphocyte</tok> <tok cat="NNS">populations</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
860594109@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">While</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Jurkat</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP" syn="COOD"><tok cat="CC">both</tok> <cons cat="VP"><tok cat="VBP">secrete</tok> <cons cat="NP" ref="i27" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBP">respond</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" ref="i27" null="RNR"></cons></cons></cons><cons cat="NP" id="i27"><cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">autocrine</tok> <tok cat="NN">loop</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">it</tok></cons> <cons cat="VP"><tok cat="VBZ">seems</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">likely</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="VBG">responding</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">differ</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="VP"><tok cat="VBG">synthesizing</tok> <cons cat="NP"><tok cat="DT">this</tok> <cons cat="NP"><tok cat="NN">cytokine</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">mononuclear</tok> <tok cat="NN">cell</tok> <tok cat="NNS">cultures</tok></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">light</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">recent</tok> <tok cat="NN">report</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Th1</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBP">lack</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IFN-gamma</tok></cons> <tok cat="NN">receptor</tok> <tok cat="NN">chain</tok></cons> <cons cat="ADJP"><tok cat="JJ">necessary</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">STAT1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNP">Pernis</tok><tok cat="COMMA">,</tok> <tok cat="NNP">A.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Gupta</tok><tok cat="COMMA">,</tok> <tok cat="NNP">S.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Gollob</tok><tok cat="COMMA">,</tok> <tok cat="NNP">K.J.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Garfein</tok><tok cat="COMMA">,</tok> <tok cat="NNP">E.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Coffman</tok><tok cat="COMMA">,</tok> <tok cat="NNP">R.L.</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Schindler</tok><tok cat="COMMA">,</tok> <tok cat="NNP">C.</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNP">Rothman</tok><tok cat="COMMA">,</tok> <tok cat="NNP">P.</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">Science</tok> <tok cat="CD">1995</tok></cons><tok cat="PERIOD">.</tok> <cons cat="NP"><tok cat="CD">269</tok><tok cat="COLON">:</tok><tok cat="CD">245</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@91@@oe@19-12-2010
860794101@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">Menopause</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">significant</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">blood</tok> <tok cat="NN">monocyte</tok> <tok cat="NN">number</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">relative</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">estrogen</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">peripheral</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@26@@oe@19-12-2010
860794102@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">PROBLEM</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">clinical</tok> <tok cat="NN">significance</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">differential</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">estrogen</tok> <tok cat="NN">receptor</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ER</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">evaluated</tok><cons cat="NP" ref="i6" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@19-12-2010
860794103@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">METHOD</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="CD">Two</tok> <tok cat="NN">color</tok> <tok cat="NN">flow</tok> <tok cat="NN">cytometry</tok> <tok cat="NN">analysis</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NNS">samples</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">young</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">postmenopausal</tok></cons> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">females</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">postmenopausal</tok> <tok cat="NNS">females</tok></cons> <cons cat="VP"><tok cat="VBN">treated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">estrogen</tok> <tok cat="NN">replacement</tok> <tok cat="NN">therapy</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
860794104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">monocyte</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">lymphocyte</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">counts</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">blood</tok> <tok cat="NN">estrogen</tok> <tok cat="NNS">levels</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">each</tok> <tok cat="NN">patient</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">determine</tok><cons cat="NP" ref="i9" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
860794105@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">RESULTS</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">During</tok> <cons cat="NP"><tok cat="NN">menopause</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" syn="COOD" role="PRD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">significant</tok> <tok cat="NN">decrease</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">percentage</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ER</tok> <tok cat="JJ">positive</tok> <tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="NN">increase</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">blood</tok> <tok cat="NN">monocyte</tok></cons> <tok cat="NN">number</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">declines</tok> <cons cat="PP"><tok cat="VBG">following</tok> <cons cat="NP"><tok cat="NN">estrogen</tok> <tok cat="NN">replacement</tok> <tok cat="NN">therapy</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">values</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">young</tok><cons cat="NP" null="QSTN"></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@19-12-2010
860794106@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NNS">CONCLUSIONS</tok><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">findings</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="UCP" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">estrogen</tok></cons> <cons cat="VP"><tok cat="VBZ">modulates</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">monocyte</tok></cons> <tok cat="NNS">numbers</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="COMP" null="ZERO"></cons><cons cat="S"><cons cat="NP" id="i11" role="SBJ"><tok cat="PRPP">its</tok> <tok cat="NNS">effects</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">ER</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NNS">monocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
860939301@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Herpesvirus</tok> <tok cat="NN">saimiri</tok> <tok cat="VBD">immortalized</tok> <tok cat="NN">gamma</tok> <tok cat="NN">delta</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">IL-12</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@12@@oe@19-12-2010
860939302@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-12</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <cons cat="NP"><tok cat="JJ">heterodimeric</tok> <tok cat="NN">cytokine</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">regulation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">differentiation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">lymphocytes</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NK</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@18@@oe@19-12-2010
860939303@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Like</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NNS">cytokines</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">IL-12</tok></cons> <cons cat="VP"><tok cat="VBZ">mediates</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <cons cat="NP"><tok cat="JJ">biologic</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high-affinity</tok> <tok cat="NNS">receptors</tok></cons> <cons cat="VP"><tok cat="VBN">expressed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="JJ">responsive</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
860939304@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><tok cat="TO">To</tok> <cons cat="NP"><tok cat="NN">date</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">large</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">receptors</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="NN">IL-12</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><cons cat="ADVP"><tok cat="RB">only</tok></cons> <tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">PBMC</tok></cons></cons> <cons cat="PP"><tok cat="VBG">following</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PHA</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
860939305@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">gain</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">further</tok> <tok cat="NN">knowledge</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-12R</tok> <tok cat="NN">complex</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">IL-12</tok></cons> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok> <tok cat="NN">pathway</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">cytotoxic</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">studied</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">number</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" id="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">had</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">transformed</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">permanent</tok> <tok cat="NN">growth</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Herpesvirus</tok> <tok cat="NN">saimiri</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">oncogenic</tok> <tok cat="NN">virus</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nonhuman</tok> <tok cat="NNS">primates</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@46@@oe@19-12-2010
860939306@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">paper</tok></cons> <cons cat="VP"><tok cat="VBZ">reports</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-12R</tok></cons></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">gamma</tok> <tok cat="NN">delta</tok> <tok cat="NN">T</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">responds</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IL-12</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">enhanced</tok> <tok cat="JJ">cytolytic</tok> <tok cat="NN">activity</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">increased</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cytolytic</tok> <tok cat="NN">effector</tok> <tok cat="NNS">molecules</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">granzyme</tok> <tok cat="NN">B</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">perforin</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@19-12-2010
860939307@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">model</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-12</tok></cons> <tok cat="NN">signal</tok> <tok cat="NN">transduction</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i9" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">confirmed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">events</tok></cons> <cons cat="VP"><tok cat="VBP">involve</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Janus</tok> <tok cat="NN">kinase</tok> <tok cat="NN">family</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">nonreceptor</tok> <tok cat="NN">tyrosine</tok> <tok cat="NNS">kinases</tok></cons></cons></cons></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">JAK2</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">TYK2</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">signal</tok> <tok cat="NN">transducer</tok> <tok cat="CC">and</tok> <tok cat="NN">activator</tok> <tok cat="IN">of</tok> <tok cat="NN">transcription</tok> <tok cat="CD">4</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@41@@oe@19-12-2010
860941801@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-13</tok></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">activation</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">JAK2</tok> <tok cat="NN">Janus</tok> <tok cat="NN">kinase</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">colon</tok> <tok cat="NN">carcinoma</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="NNS">similarities</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-4</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-13</tok></cons></cons> <tok cat="NN">signaling</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@19-12-2010
860941802@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP"><tok cat="VBN">reported</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-13R</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">share</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">component</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IL-4R</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@19-12-2010
860941803@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">report</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-13</tok></cons></cons> <cons cat="VP"><tok cat="VBP">share</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">signaling</tok> <tok cat="NNS">events</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">colon</tok> <tok cat="NN">carcinoma</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">HT-29</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">WiDr</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
860941804@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-13</tok></cons> <cons cat="VP"><tok cat="VBD">caused</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">rapid</tok> <tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="QP"><tok cat="CD">three</tok> <tok cat="IN">out</tok> <tok cat="IN">of</tok> <tok cat="CD">four</tok></cons> <tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">known</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Janus</tok> <tok cat="NN">family</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">kinases</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NNS">JAKs</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
860941805@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i40" role="SBJ"><tok cat="NN">JAK2</tok> <tok cat="NN">kinase</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">rapidly</tok></cons> <cons cat="VP"><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBN">phosphorylated</tok> <cons cat="NP" ref="i40" null="NONE"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i40" null="NONE"></cons></cons></cons><cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">IL-13</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
860941806@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" tpc="TPC" id="i42" adv="TMP"><tok cat="IN">Within</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">1</tok> <tok cat="NN">min</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">activation</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i41" role="SBJ"><tok cat="NN">JAK2</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">phosphorylated</tok><cons cat="NP" ref="i41" null="NONE"></cons><cons cat="PP" ref="i42" null="T"></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBD">peaked</tok> <cons cat="PP" adv="TMP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="CD">10</tok> <tok cat="NN">min</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
860941807@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">IL-13</tok></cons> <cons cat="VP"><tok cat="VBD">phosphorylated</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">insulin</tok> <tok cat="NN">response</tok> <tok cat="NN">substrate-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">IL-4R</tok> <tok cat="NN">p140</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">JAK1</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Tyk2</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="NN">JAK3</tok> <tok cat="NN">kinase</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
860941808@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-4</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">stimulated</tok> <cons cat="NP"><cons cat="QP"><tok cat="DT">all</tok> <tok cat="CD">three</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNS">kinases</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNS">substrates</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">unlike</tok> <cons cat="FRAG"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">immune</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">IL-4</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">involve</tok> <cons cat="NP"><tok cat="NN">JAK3</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">signaling</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">colon</tok> <tok cat="NN">cancer</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
860941809@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Furthermore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">JAK2</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">IL-4R</tok> <tok cat="NN">p140</tok></cons></cons> <cons cat="PP" adv="TMP"><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">before</tok> <cons cat="NP" ref="i44" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="PP"><tok cat="IN">after</tok> <cons cat="NP" ref="i44" null="RNR"></cons></cons></cons><cons cat="NP" id="i44"><cons cat="NP"><tok cat="NN">stimulation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IL-13</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
860941810@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="CC">Both</tok> <cons cat="NP"><tok cat="NN">IL-13</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-4</tok></cons></cons> <cons cat="VP"><tok cat="VBD">induced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">phosphorylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">IL-4</tok> <tok cat="NN">STAT</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">STAT6</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">STAT1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">STAT3</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">STAT5</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
860941811@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">125I-IL-13</tok></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">bind</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">colon</tok> <tok cat="NN">cancer</tok> <tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">unlabeled</tok> <tok cat="NN">IL-13</tok></cons> <cons cat="VP"><tok cat="VBD">competed</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">125I-IL-4</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
860941812@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRPP">Our</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">IL-13</tok></cons> <cons cat="VP"><tok cat="VBZ">utilizes</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-4R</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">JAK2</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">IL-4R</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-13R</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">colon</tok> <tok cat="NN">cancer</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
861011601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok> <tok cat="JJ">N-terminal</tok> <tok cat="NN">kinase</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">bacterial</tok> <tok cat="JJ">lipopolysaccharide-stimulated</tok> <tok cat="NNS">macrophages</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@10@@oe@19-12-2010
861011602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">bacterial</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">lipopolysaccharide</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">LPS</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">induces</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">genes</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">encode</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">proinflammatory</tok> <tok cat="NNS">regulators</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immune</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@23@@oe@19-12-2010
861011603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Previous</tok> <tok cat="NN">work</tok></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">suggested</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="NN">activator</tok> <tok cat="NN">protein</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">AP-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="CD">one</tok> <tok cat="JJ">LPS-induced</tok> <tok cat="NN">event</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">mediates</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
861011604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="JJ">Consistent</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">notion</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">found</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">LPS</tok></cons> <cons cat="VP"><tok cat="VBD">stimulated</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">AP-1-mediated</tok> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="VBN">transfected</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">macrophage</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">RAW</tok> <tok cat="CD">264.7</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
861011605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">As</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="NN">activity</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">part</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">c-Jun</tok> <tok cat="JJ">N-terminal</tok> <tok cat="NN">kinase</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">JNK</tok></cons><tok cat="RRB">)</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">phosphorylates</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">subsequently</tok></cons> <cons cat="VP"><tok cat="VBZ">increases</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">activity</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">examined</tok> <cons cat="SBAR"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">LPS</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">macrophages</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">this</tok> <cons cat="NP"><tok cat="NN">kinase</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@19-12-2010
861011606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">LPS</tok> <tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">RAW</tok> <tok cat="CD">264.7</tok> <tok cat="NNS">cells</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="JJ">murine</tok> <tok cat="NN">bone</tok> <tok cat="JJ">marrow-derived</tok> <tok cat="NNS">macrophages</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">monocyte</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">THP-1</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">rapid</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">p46</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">p54</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">isoforms</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">JNK</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
861011607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADJP"><tok cat="JJ">wild-type</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="JJ">rough</tok></cons> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="JJ">mutant</tok> <tok cat="NNS">forms</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">LPS</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">synthetic</tok> <tok cat="NN">lipid</tok> <tok cat="NN">A</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">JNK</tok></cons> <tok cat="NN">activation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">pretreatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">tyrosine</tok> <tok cat="NN">kinase</tok></cons> <tok cat="NN">inhibitor</tok> <tok cat="NN">herbimycin</tok> <tok cat="NN">A</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">response</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
861011608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NN">Binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">LPS-LPS</tok> <tok cat="NN">binding</tok> <tok cat="NN">protein</tok> <tok cat="LRB">(</tok><tok cat="NN">LBP</tok><tok cat="RRB">)</tok> <tok cat="NNS">complexes</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CD14</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">surface</tok> <tok cat="NN">receptor</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">mediates</tok> <cons cat="NP"><tok cat="JJ">many</tok> <tok cat="NN">LPS</tok> <tok cat="NNS">responses</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">crucial</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">pretreatment</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">THP-1</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">monoclonal</tok> <tok cat="NN">antibody</tok> <tok cat="NN">60b</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">blocks</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">binding</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">JNK</tok></cons> <tok cat="NN">activation</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@19-12-2010
861011609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">LPS</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">JNK</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">monocyte/macrophage</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" role="PRD"><cons cat="ADJP"><tok cat="NN">CD14-</tok> <cons cat="ADJP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="NN">protein</tok> <tok cat="NN">tyrosine</tok> <tok cat="JJ">phosphorylation-dependent</tok></cons></cons> <tok cat="NN">event</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i11"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">mediate</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">early</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">regulating</tok> <cons cat="NP"><tok cat="JJ">LPS-triggered</tok> <tok cat="NN">gene</tok> <tok cat="NN">induction</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
861724401@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="JJ">C-terminal</tok> <cons cat="NP"><tok cat="VBG">activating</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">inhibitory</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">domains</tok></cons></cons> <cons cat="VP"><tok cat="VBP">determine</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">transactivation</tok> <tok cat="NN">potential</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">BSAP</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Pax-5</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">Pax-2</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">Pax-8</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@19-12-2010
861724402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Pax-5</tok></cons> <cons cat="VP"><tok cat="VBZ">encodes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="NP"><tok cat="NN">BSAP</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">plays</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">essential</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">early</tok> <tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">development</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">midbrain</tok> <tok cat="NN">patterning</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@19-12-2010
861724403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">study</tok></cons></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">analysed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">structural</tok> <tok cat="NNS">requirements</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">BSAP</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
861724404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><cons cat="ADJP"><tok cat="FW">In</tok> <tok cat="FW">vitro</tok></cons> <tok cat="NN">mutagenesis</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">transfection</tok> <cons cat="NP" ref="i21" null="RNR"></cons></cons><cons cat="NP" id="i21"><tok cat="NNS">experiments</tok></cons></cons> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">C-terminal</tok> <tok cat="JJ">serine/threonine/proline-rich</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">BSAP</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">potent</tok> <cons cat="NP"><tok cat="NN">transactivation</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="CD">55</tok> <tok cat="NN">amino</tok> <tok cat="NNS">acids</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i5"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i5" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">active</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">promoter</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">enhancer</tok> <cons cat="NP" ref="i22" null="RNR"></cons></cons><cons cat="NP" id="i22"><tok cat="NNS">positions</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
861724405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><tok cat="DT">This</tok> <cons cat="NP"><tok cat="NN">transactivation</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">inactivated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="RB">naturally</tok> <tok cat="VBG">occurring</tok></cons> <tok cat="NN">frameshift</tok> <tok cat="NN">mutation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">one</tok> <cons cat="NP"><tok cat="NN">PAX-5</tok> <tok cat="NN">allele</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">lymphoblastic</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">cell</tok> <tok cat="NN">line</tok> <tok cat="NN">REH</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
861724406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transactivation</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">negatively</tok></cons> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">adjacent</tok> <tok cat="NNS">sequences</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">extreme</tok> <tok cat="NN">C-terminus</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
861724407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="VBG">activating</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">inhibitory</tok> <cons cat="NP" ref="i23" null="RNR"></cons></cons><cons cat="NP" id="i23"><tok cat="NNS">domains</tok></cons></cons> <cons cat="VP"><tok cat="VBP">function</tok> <cons cat="ADVP"><tok cat="RB">together</tok></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">independent</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">module</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">different</tok> <tok cat="NN">cell</tok> <tok cat="NNS">types</tok></cons></cons></cons></cons> <cons cat="SBAR" func="ADV"><tok cat="IN">as</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">fusion</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GAL4</tok> <tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <tok cat="NN">domain</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
861724408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">same</tok> <tok cat="NN">arrangement</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="ADVP"><tok cat="RB">positively</tok></cons> <cons cat="NP" ref="i25" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><cons cat="ADVP"><tok cat="RB">negatively</tok></cons> <cons cat="NP" ref="i25" null="RNR"></cons></cons><cons cat="NP" id="i25"><tok cat="VBG">acting</tok> <tok cat="NNS">sequences</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">has</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">conserved</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">mammalian</tok> <tok cat="NN">Pax-2</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP" null="QSTN"></cons><cons cat="NP"><tok cat="NN">Pax-8</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">zebrafish</tok> <tok cat="NN">Pax-b</tok></cons> <cons cat="NP" null="QSTN"></cons></cons><cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">sea</tok> <tok cat="NN">urchin</tok> <tok cat="NN">Pax-258</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
861724409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">competence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">subfamily</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Pax</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">determined</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">C-terminal</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">module</tok></cons></cons> <cons cat="VP"><tok cat="VBN">composed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBG">activating</tok> <cons cat="NP" ref="i26" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">inhibitory</tok> <cons cat="NP" ref="i26" null="RNR"></cons></cons><cons cat="NP" id="i26"><tok cat="NNS">sequences</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
861776601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Ubiquitinylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Jun</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Fos</tok></cons></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">using</tok> <cons cat="NP"><tok cat="VBN">reconstituted</tok> <tok cat="VBG">ubiquitinylating</tok> <tok cat="NNS">enzymes</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@12@@oe@19-12-2010
861776602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" syn="COOD" id="i5" role="SBJ"><tok cat="JJ">Recombinant</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Fos</tok></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">ubiquitinylated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">ubiquitin</tok> <tok cat="NN">carrier</tok> <tok cat="NNS">enzymes</tok></cons></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">E214K</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">E220K</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">E232K</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">ubiquitin-activating</tok> <tok cat="NN">enzyme</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">E1</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@27@@oe@19-12-2010
861776603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ubiquitin</tok> <tok cat="NN">protein</tok> <tok cat="NN">ligase</tok> <tok cat="NN">E3</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">substantially</tok></cons> <cons cat="VP"><tok cat="VBD">enhanced</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">E214K-mediated</tok> <tok cat="NN">ubiquitinylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Jun</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Fos</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
861776604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="VBN">Truncated</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Fos</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="JJ">mutant</tok> <tok cat="NNS">proteins</tok></cons></cons> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">wbJun</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">wbFos</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">ubiquitinylated</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NNS">conditions</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="COMP" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">sites</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ubiquitinylation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">dimerization</tok> <cons cat="NP" ref="i27" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">DNA</tok> <tok cat="NN">binding</tok> <cons cat="NP" ref="i27" null="RNR"></cons></cons><cons cat="NP" id="i27"><tok cat="NNS">domains</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Jun</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Fos</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@37@@oe@19-12-2010
861776605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">E3-dependent</tok> <tok cat="NN">ubiquitinylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">heterodimerization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">c-Fos</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
861776606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="IN">Further</tok> <tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">E220K</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="RB">significantly</tok></cons> <cons cat="VP"><tok cat="VBD">enhanced</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">ubiquitinylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">heterodimer</tok></cons></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">regulatory</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">E220K</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
861776607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" id="i9" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="VBN">Polyubiquitinylated</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">wbFos</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">wbJun</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="CC">but</tok> <tok cat="RB">not</tok></cons> <cons cat="NP"><tok cat="JJ">E220K-ubiquitinylated</tok> <tok cat="NN">c-Jun</tok></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADVP"><tok cat="RB">readily</tok></cons> <cons cat="VP"><tok cat="VBN">degraded</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">ATP-dependent</tok> <tok cat="CD">26</tok> <tok cat="NN">S</tok> <tok cat="JJ">multicatalytic</tok> <tok cat="NNS">proteases</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
861776608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="UCP" syn="COOD"><cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i10" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">temporal</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Jun</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Fos</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i10" null="NONE"></cons><cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ubiquitinylation</tok> <tok cat="NNS">pathways</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="COMP" null="ZERO"></cons><cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">ubiquitinylation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Jun</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">c-Fos</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">turn</tok></cons></cons> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">regulated</tok> <cons cat="NP" ref="i11" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">heterodimerization</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="PRP">them</tok></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">cooperation</tok></cons> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">E220K</tok> <cons cat="NP" ref="i28" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">E3</tok> <cons cat="NP" ref="i28" null="RNR"></cons></cons><cons cat="NP" id="i28"><tok cat="JJ">mediated</tok> <tok cat="NN">polyubiquitinylation</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@48@@oe@19-12-2010
861845601@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">A</tok> <tok cat="JJ">new</tok> <tok cat="JJ">variant</tok> <tok cat="NN">translocation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">promyelocytic</tok> <tok cat="NN">leukaemia</tok></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">molecular</tok> <tok cat="NN">characterization</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">clinical</tok> <tok cat="NN">correlation</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@15@@oe@19-12-2010
861845602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">Translocation</tok> <tok cat="NN">t(15;17)(q22;q21)</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">an</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="VBN">acquired</tok></cons> <cons cat="ADJP"><tok cat="JJ">clonal</tok></cons> <cons cat="ADJP"><tok cat="JJ">cytogenetic</tok></cons></cons> <tok cat="NN">change</tok></cons> <cons cat="ADJP"><tok cat="JJ">present</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">almost</tok> <tok cat="DT">all</tok></cons> <tok cat="NNS">cases</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">acute</tok> <tok cat="JJ">promelocytic</tok> <tok cat="NN">leukemia</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">APL</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@21@@oe@19-12-2010
861845603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">molecular</tok> <tok cat="JJ">genetic</tok> <tok cat="NN">basis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">translocation</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">supports</tok> <cons cat="NP"><cons cat="NP"><tok cat="PRPP">its</tok> <tok cat="JJ">integral</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">pathogenesis</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
861845604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">describe</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">patient</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">APL</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHPP" id="i1"><tok cat="IN">in</tok> <cons cat="WHNP"><tok cat="WP">whom</tok></cons></cons> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">leukaemic</tok> <tok cat="NN">clone</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">characterized</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">true</tok> <tok cat="NN">variant</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">classical</tok> <tok cat="NN">t(15;17)</tok></cons></cons></cons></cons></cons><cons cat="PP" ref="i1" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
861845605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">patient</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i6"><tok cat="WPP">whose</tok> <tok cat="NN">disease</tok></cons> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">numerous</tok></cons> <cons cat="ADJP"><tok cat="JJ">atypical</tok></cons> <cons cat="ADJP"><tok cat="JJ">clinical</tok></cons></cons> <tok cat="NNS">features</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBD">had</tok> <cons cat="NP"><tok cat="NN">t(11;17)(q13;121)</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@13@@oe@19-12-2010
861845606@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="NN">chromosome</tok> <tok cat="CD">17</tok> <tok cat="NN">breakpoint</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">localized</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">intron</tok> <tok cat="CD">2</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">RARA</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">Southern</tok> <tok cat="NN">blotting</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="EX">there</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">no</tok> <tok cat="NN">evidence</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">molecular</tok> <tok cat="NN">level</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">rearrangement</tok></cons> <cons cat="PP"><tok cat="IN">at</tok> <cons cat="NP"><tok cat="NN">PML</tok> <tok cat="NN">locus</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
861845607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="NNS">data</tok><tok cat="COMMA">,</tok></cons> <cons cat="PP"><tok cat="IN">along</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">previous</tok> <tok cat="NNS">reports</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">rare</tok> <tok cat="JJ">variant</tok> <tok cat="NNS">translocations</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">APL</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="VP"><tok cat="VBP">indicate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="SBAR" func="ADV"><tok cat="IN">while</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">dysregulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">RARA</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">gene</tok> <tok cat="NN">fusion</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">essential</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">APL</tok> <tok cat="NN">phenotype</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">the</tok> <tok cat="JJ">particular</tok> <tok cat="NN">fusion</tok> <tok cat="NN">partner</tok></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">determine</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">clinicopathological</tok> <tok cat="NNS">aspects</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="VBG">including</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">presentation</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">response</tok></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">treatment</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">all-trans</tok> <tok cat="JJ">retinoic</tok> <tok cat="NN">acid</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">ATRA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">prognosis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@57@@oe@19-12-2010
861845608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">heterogeneity</tok></cons> <cons cat="VP"><tok cat="VBZ">suggests</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">variant</tok> <cons cat="NP"><tok cat="NN">fusion</tok> <tok cat="NNS">partners</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">RARA</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">APL</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">encode</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">factors</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">properties</tok></cons> <cons cat="ADJP" syn="COOD"><tok cat="CC">both</tok> <cons cat="ADJP"><tok cat="JJ">common</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons></cons><tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">distinct</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons></cons><cons cat="NP" id="i20"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">PML</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
861845609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">Investigation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">factors</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">promises</tok> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">shed</tok> <cons cat="NP"><tok cat="NN">light</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">complex</tok> <tok cat="NN">development</tok> <tok cat="NNS">pathways</tok></cons> <cons cat="VP"><tok cat="VBN">involved</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">haematopoiesis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
861983401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Regulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="IN">of</tok> <tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">stably</tok> <tok cat="VBN">transfected</tok></cons> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">cell</tok> <tok cat="NNS">clones</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@16@@oe@19-12-2010
861983402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="CD">Two</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">cell</tok> <tok cat="NNS">clones</tok></cons></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="ADVP"><tok cat="RB">stably</tok></cons> <cons cat="VP"><tok cat="VBN">transfected</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">reporter</tok> <tok cat="NN">vector</tok></cons></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok> <tok cat="IN">of</tok> <tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NFAT</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@24@@oe@19-12-2010
861983403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">Upon</tok> <cons cat="NP"><tok cat="NN">stimulation</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">they</tok></cons> <cons cat="VP"><tok cat="VBP">express</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="VBN">secreted</tok></cons> <cons cat="ADJP"><tok cat="NN">heat</tok> <tok cat="JJ">stable</tok></cons></cons> <cons cat="NP"><tok cat="JJ">placental</tok> <tok cat="NN">alkaline</tok> <tok cat="NN">phosphatase</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
861983404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">With</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">clones</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">demonstrated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="NN">NFAT</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><cons cat="NP"><tok cat="NN">phorbol</tok> <tok cat="NN">12-myristate</tok> <tok cat="NN">13-acetate</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">ionomycin</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" syn="COOD" role="LGS"><tok cat="CC">both</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">cyclosporin</tok> <tok cat="NN">A</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CsA</tok></cons><tok cat="RRB">)</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">IC50</tok></cons> <cons cat="ADJP"><tok cat="JJ">=</tok> <cons cat="NP"><tok cat="CD">8</tok> <tok cat="NN">nM</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">FK506</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP"><tok cat="NN">IC50</tok></cons> <cons cat="ADJP"><tok cat="JJ">=</tok> <cons cat="NP"><tok cat="CD">160</tok> <tok cat="NN">pM</tok></cons></cons></cons><tok cat="RRB">)</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">presumably</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">calcineurin</tok></cons> <tok cat="NN">activity</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@47@@oe@19-12-2010
861983405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Selective</tok> <tok cat="NN">phosphatase</tok> <tok cat="NNS">inhibitors</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">phosphatase</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PP1</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP" null="QSTN"></cons><cons cat="NP"><tok cat="NN">2A</tok></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">PP2A</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">do</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">inhibit</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">calcineurin</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">okadaic</tok> <tok cat="NN">acid</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">calyculin</tok> <tok cat="NN">A</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBD">inhibited</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">NFAT</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">IC50s</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="CD">87</tok> <tok cat="NN">nM</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="CD">4</tok> <tok cat="NN">nM</tok></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">respectively</tok></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="JJ">okadaic</tok> <tok cat="NN">acid</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">related</tok> <tok cat="NNS">inhibitors</tok></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">block</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NFAT</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PP1</tok></cons><tok cat="COMMA">,</tok> <cons cat="CONJP"><tok cat="RB">instead</tok> <tok cat="IN">of</tok></cons> <cons cat="NP"><tok cat="NN">PP2A</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@65@@oe@19-12-2010
861983406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">NFAT</tok></cons> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">inhibited</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">agents</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i9"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">increase</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cAMP</tok> <tok cat="NNS">concentrations</tok></cons> <cons cat="PP"><tok cat="JJ">such</tok> <tok cat="IN">as</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="JJ">dibutyryl</tok> <tok cat="NN">cAMP</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">forskolin</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">prostaglandin</tok> <tok cat="NN">E2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
861983407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">These</tok> <tok cat="JJ">stable</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NN">cell</tok> <tok cat="NNS">clones</tok></cons></cons> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">convenient</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">sensitive</tok></cons></cons> <tok cat="NN">tool</tok></cons> <cons cat="SBAR"><cons cat="WHADVP" id="i19" null="ZERO"></cons><cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">study</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">NFAT</tok></cons> <tok cat="NN">regulation</tok></cons><cons cat="ADVP" ref="i19" null="T"></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
862054601@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i28" role="SBJ"><cons cat="NP"><tok cat="JJ">Age-related</tok> <tok cat="NNS">decreases</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i28" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">impaired</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NNS">factors</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
862054602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="SBAR"><tok cat="IN">Although</tok> <cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NNS">factors</tok> <tok cat="NN">AP-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NF-AT</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">important</tok></cons> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">normal</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRP">it</tok></cons> <cons cat="SBAR" ref="i30" null="EXP"></cons></cons><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unknown</tok></cons> <cons cat="SBAR" id="i30"><tok cat="IN">if</tok> <cons cat="S"><cons cat="NP" id="i29" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">age-related</tok> <tok cat="NN">decline</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i29" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">aberrancies</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="JJ">regulatory</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@48@@oe@19-12-2010
862054603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">current</tok> <tok cat="NNS">studies</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i31" role="SBJ"><cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">elderly</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">mean</tok></cons> <cons cat="NP" role="PRD"><tok cat="CD">78</tok> <tok cat="NNS">years</tok></cons></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">young</tok> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">mean</tok></cons> <cons cat="NP" role="PRD"><tok cat="CD">37</tok> <tok cat="NNS">years</tok></cons></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="NNS">humans</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">measured</tok> <cons cat="NP" ref="i31" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">cultures</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PHA</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PHA</tok></cons> <tok cat="CC">plus</tok> <cons cat="NP"><tok cat="NN">PMA</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBN">crosslinked</tok> <tok cat="JJ">anti-CD3</tok> <tok cat="NN">mAB</tok> <tok cat="NN">OKT3</tok></cons> <tok cat="CC">plus</tok> <cons cat="NP"><tok cat="NN">PMA</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">or</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PMA</tok></cons> <tok cat="CC">plus</tok> <cons cat="NP"><tok cat="NN">ionomycin</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@49@@oe@19-12-2010
862054604@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i33" role="SBJ"><cons cat="NP"><tok cat="JJ">Substantial</tok> <tok cat="NNS">decreases</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">production</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" ref="i33" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NNS">cultures</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="QP"><tok cat="CD">7</tok> <tok cat="IN">of</tok> <tok cat="CD">12</tok></cons> <tok cat="JJ">elderly</tok> <tok cat="NNS">individuals</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="NN">response</tok> <tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">different</tok> <tok cat="NNS">stimuli</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">IL-2</tok></cons> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="VBN">stimulated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="JJ">elderly</tok> <tok cat="NNS">individuals</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">equivalent</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="VP"><tok cat="VBN">observed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">stimulated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">young</tok> <tok cat="NNS">subjects</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@50@@oe@19-12-2010
862054605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">Analyses</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">electrophoretic</tok> <tok cat="NN">DNA</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NNS">assays</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i35" role="SBJ"><cons cat="NP"><tok cat="VBN">decreased</tok> <tok cat="NN">IL-2</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">stimulated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">elderly</tok> <tok cat="NNS">individuals</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">closely</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i35" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">impairments</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@19-12-2010
862054606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="NP"><tok cat="NN">contrast</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">elderly</tok> <tok cat="NNS">subjects</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">production</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">exhibited</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">normal</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@22@@oe@19-12-2010
862054607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="NN">addition</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NNS">results</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">competition</tok> <tok cat="NNS">experiments</tok></cons> <cons cat="VP"><tok cat="VBG">analyzing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">normal</tok> <tok cat="NNS">components</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">age-related</tok> <tok cat="NNS">reductions</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">stimulus-dependent</tok> <tok cat="NN">NF-AT</tok> <tok cat="NNS">complexes</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">corresponded</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="ADJP"><tok cat="JJ">slow</tok> <tok cat="VBG">migrating</tok></cons> <tok cat="NNS">complexes</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i36"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i36" id="i37" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">composed</tok> <cons cat="NP" ref="i37" null="NONE"></cons><cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">c-Fos/c-Jun</tok> <tok cat="NN">AP-1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@19-12-2010
862054608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i38" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">resting</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">stimulated</tok></cons></cons> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">reduced</tok> <cons cat="NP" ref="i38" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><tok cat="JJ">certain</tok> <tok cat="JJ">elderly</tok> <tok cat="NNS">individuals</tok></cons></cons></cons></cons></cons></cons><tok cat="COLON">;</tok></cons> <cons cat="S"><cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">alterations</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF</tok> <tok cat="NN">kappa</tok> <tok cat="NN">B</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">correlate</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNS">changes</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">expression</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@35@@oe@19-12-2010
862054609@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">results</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" id="i39" role="SBJ"><cons cat="NP"><tok cat="JJ">age-related</tok> <tok cat="NNS">impairments</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">AP-1</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADVP"><tok cat="RB">closely</tok></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" ref="i39" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="VBN">decreased</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">further</tok></cons> <tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NNS">aberrancies</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">signaling</tok> <tok cat="NNS">pathways</tok></cons></cons> <cons cat="ADJP"><tok cat="JJ">important</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="ADJP"><tok cat="RB">transcriptionally</tok> <tok cat="JJ">active</tok></cons> <tok cat="NN">c-Fos/c-Jun</tok> <tok cat="NN">AP-1</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">contribute</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">impaired</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">NF-AT</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@50@@oe@19-12-2010
862297101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">cAMP</tok> <tok cat="NN">inducibility</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NN">repressor</tok></cons> <cons cat="NP"><tok cat="NN">ICER</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="VBG">developing</tok> <cons cat="NP" null="QSTN"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="JJ">mature</tok> <tok cat="JJ">human</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons> <cons cat="S"><tok cat="LRB">[</tok><cons cat="NP" role="SBJ"><tok cat="VBN">published</tok> <tok cat="NN">erratum</tok></cons> <cons cat="VP"><tok cat="VBZ">appears</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NNP">Proc</tok> <tok cat="NNP">Natl</tok> <tok cat="NNP">Acad</tok> <tok cat="NNP">Sci</tok> <tok cat="NNP">U</tok> <tok cat="NNP">S</tok> <tok cat="NNP">A</tok></cons> <cons cat="NP"><tok cat="CD">1996</tok> <tok cat="NNP">Jul</tok> <tok cat="CD">23</tok></cons><tok cat="COLON">;</tok><cons cat="NP"><tok cat="CD">93</tok><tok cat="LRB">(</tok><tok cat="CD">15</tok><tok cat="RRB">)</tok></cons><tok cat="COLON">:</tok><cons cat="NP"><tok cat="CD">8154</tok></cons></cons></cons></cons><tok cat="RRB">]</tok></cons></cons></cons>@@@@1@36@@oe@19-12-2010
862297102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Stimulation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cAMP-dependent</tok> <tok cat="NN">signaling</tok> <tok cat="NN">pathway</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">exerts</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">inhibitory</tok> <tok cat="NN">effect</tok></cons> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">proliferation</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">effector</tok> <tok cat="NNS">functions</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@19-12-2010
862297103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">ability</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">form</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">high</tok> <tok cat="JJ">intracellular</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">cAMP</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">acquired</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP" adv="TMP"><tok cat="IN">during</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">development</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">human</tok> <tok cat="NN">thymus</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">retained</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">majority</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">mature</tok> <cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">T</tok> <tok cat="NNS">lymphocytes</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
862297104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">elevated</tok> <tok cat="NN">cAMP</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBP">correlate</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">potent</tok> <tok cat="JJ">transcriptional</tok> <tok cat="NN">repressor</tok></cons></cons> <cons cat="NP"><cons cat="NP"><tok cat="NN">ICER</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="JJ">inducible</tok> <tok cat="NN">cAMP</tok> <tok cat="JJ">early</tok> <tok cat="NN">repressor</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="ADVP" adv="TMP"><tok cat="RB">previously</tok></cons> <cons cat="VP"><tok cat="VBN">described</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">hypothalamic-pituitary-gonadal</tok> <tok cat="NN">axis</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@33@@oe@19-12-2010
862297105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Further</tok></cons><tok cat="COMMA">,</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">transcriptional</tok> <tok cat="NNS">assays</tok></cons> <cons cat="ADJP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">ICER</tok></cons> <cons cat="VP"><tok cat="VBZ">inhibits</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">calcineurin-mediated</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="CD">2</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Tax-mediated</tok> <tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="JJ">T-lymphotropic</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">I</tok> <tok cat="LRB">(</tok><tok cat="NN">HTLV-I</tok><tok cat="RRB">)</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@19-12-2010
862297106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Thus</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ICER</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">may</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">important</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">cAMP-induced</tok> <tok cat="NN">quiescence</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">persistent</tok> <tok cat="NN">latency</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HTLV-I</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
862722601@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">antigen</tok> <tok cat="CD">2</tok> <tok cat="LRB">(</tok><tok cat="NN">EBNA2</tok><tok cat="RRB">)</tok><tok cat="NN">-oestrogen</tok> <tok cat="NN">receptor</tok> <tok cat="NN">fusion</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBP">complement</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">EBNA2-deficient</tok> <tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok> <tok cat="NN">strain</tok> <tok cat="NN">P3HR1</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transformation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="VP"><tok cat="VBP">suppress</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">growth</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">lymphoma</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@35@@oe@19-12-2010
862722602@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">develop</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">transformation</tok> <tok cat="NN">system</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">conditional</tok> <cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">antigen</tok> <tok cat="CD">2</tok> <tok cat="LRB">(</tok><tok cat="NN">EBNA2</tok><tok cat="RRB">)</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">fused</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">hormone</tok> <tok cat="NN">binding</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">oestrogen</tok> <tok cat="NN">receptor</tok></cons></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">N</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">C</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">terminus</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">EBNA2</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@37@@oe@19-12-2010
862722603@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">promoter</tok> <tok cat="NN">transactivation</tok> <cons cat="NP" ref="i27" null="RNR"></cons></cons><cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><tok cat="JJ">primary</tok> <tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">transformation</tok> <cons cat="NP" ref="i27" null="RNR"></cons></cons><cons cat="NP" id="i27"><tok cat="NNS">assays</tok></cons></cons></cons> <cons cat="NP" id="i6" role="SBJ"><tok cat="DT">these</tok> <tok cat="JJ">chimeric</tok> <cons cat="NP"><tok cat="NN">EBNA2</tok></cons> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i6" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">substitute</tok> <cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><tok cat="JJ">wild-type</tok> <tok cat="NN">EBNA2</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">oestrogen</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
862722604@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">provide</tok> <cons cat="NP"><tok cat="NN">evidence</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">transformation</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">result</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">double</tok> <tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">cell</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">virions</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">P3HR1</tok> <tok cat="NN">virus</tok> <tok cat="NN">genome</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">mini-EBV</tok> <tok cat="NN">plasmid</tok></cons></cons> <cons cat="VP"><tok cat="VBG">carrying</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">chimeric</tok> <cons cat="NP"><tok cat="NN">EBNA2</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@19-12-2010
862722605@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Unexpectedly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">same</tok> <tok cat="NN">EBNA2-oestrogen</tok> <tok cat="NN">receptor</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="JJ">established</tok> <tok cat="JJ">human</tok> <tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">lymphoma</tok> <tok cat="NNS">lines</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">resulted</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">growth</tok> <tok cat="NN">retardation</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">growth</tok> <tok cat="NN">arrest</tok></cons></cons> <cons cat="PP"><tok cat="IN">upon</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">addition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">oestrogen</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
862722606@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP"><tok cat="IN">By</tok> <cons cat="S" func="NOM"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">titrating</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">oestrogen</tok> <tok cat="NN">concentration</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="RB">stably</tok> <tok cat="VBN">transfected</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" syn="COOD" id="i8" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">growth</tok> <tok cat="NN">retarding</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="VBG">transactivating</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">chimeric</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">dissociated</tok><cons cat="NP" ref="i8" null="NONE"></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
862722607@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">propose</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">growth</tok> <tok cat="NN">inhibition</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">established</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">lymphoma</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">novel</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">EBNA2</tok></cons></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" id="i9" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">has</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VBN">been</tok> <cons cat="VP"><tok cat="VBN">detected</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">absence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">an</tok> <tok cat="JJ">inducible</tok> <tok cat="NN">system</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@30@@oe@19-12-2010
862722608@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="PRP">It</tok></cons> <cons cat="SBAR" ref="i30" null="EXP"></cons></cons><cons cat="VP"><tok cat="VBZ">remains</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">open</tok></cons> <cons cat="SBAR" id="i30"><tok cat="IN">whether</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">growth</tok> <tok cat="NN">retarding</tok> <tok cat="NN">property</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">EBNA2-oestrogen</tok> <tok cat="NN">receptor</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">B</tok> <tok cat="NN">cell</tok> <tok cat="NN">lymphoma</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">due</tok> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><cons cat="ADJP"><tok cat="RB">unphysiologically</tok> <tok cat="JJ">high</tok></cons> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">chimeric</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interference</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">cellular</tok> <tok cat="NN">programme</tok></cons> <cons cat="VP"><tok cat="VBG">driving</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">proliferation</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">these</tok> <cons cat="NP"><tok cat="NN">cell</tok> <tok cat="NNS">lines</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@43@@oe@19-12-2010
862769501@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="NN">Identification</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">herpesvirus</tok> <tok cat="NN">Saimiri</tok> <tok cat="JJ">cis-acting</tok> <tok cat="NN">DNA</tok> <tok cat="NN">fragment</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i4"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i4" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">permits</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">stable</tok> <tok cat="NN">replication</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NNS">episomes</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="VBN">transformed</tok> <cons cat="NP"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@19@@oe@19-12-2010
862769502@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="NP" id="i5" role="SBJ"><tok cat="NN">Herpesvirus</tok> <tok cat="NN">saimiri</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">lymphotropic</tok> <tok cat="NN">herpesvirus</tok></cons> <cons cat="ADJP"><tok cat="JJ">capable</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="S" func="NOM"><cons cat="NP" ref="i5" role="SBJ" null="NONE"></cons><cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBG">immortalizing</tok> <cons cat="NP" ref="i30" null="RNR"></cons><cons cat="ADVP" ref="i31" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBG">transforming</tok> <cons cat="NP" ref="i30" null="RNR"></cons><cons cat="ADVP" ref="i31" null="RNR"></cons></cons><cons cat="NP" id="i30"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="ADVP" syn="COOD" id="i31"><tok cat="CC">both</tok> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vitro</tok></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="FW">in</tok> <tok cat="FW">vivo</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@20@@oe@19-12-2010
862769503@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" syn="COOD" role="SBJ"><cons cat="NP"><tok cat="VBN">Immortalized</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="VBN">transformed</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="VP"><tok cat="VBP">harbor</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NNS">copies</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">genome</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="VBG">persisting</tok> <tok cat="NN">genome</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@17@@oe@19-12-2010
862769504@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i15" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">mapping</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cis-acting</tok> <tok cat="JJ">genetic</tok> <tok cat="JJ">cis-acting</tok> <tok cat="NN">segment</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">oriP</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">viral</tok></cons> <cons cat="ADJP"><tok cat="JJ">episomal</tok></cons></cons> <tok cat="NN">maintenance</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">reported</tok> <cons cat="NP" ref="i15" null="NONE"></cons><cons cat="ADVP"><tok cat="RB">here</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@20@@oe@19-12-2010
862769505@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="JJ">Viral</tok> <tok cat="NN">DNA</tok> <tok cat="NNS">fragments</tok></cons> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="ADVP"><tok cat="RB">potentially</tok></cons> <cons cat="VP"><tok cat="VBP">contain</tok> <cons cat="NP"><tok cat="NN">oriP</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">cloned</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">into</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="NN">plasmid</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i8"><tok cat="WDT">that</tok></cons> <cons cat="S"><cons cat="NP" ref="i8" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">hygromycin</tok> <tok cat="NN">resistance</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
862769506@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="PP" adv="TMP"><tok cat="IN">After</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">several</tok> <tok cat="NN">round</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">subcloning</tok></cons> <cons cat="VP"><tok cat="VBN">followed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">transfection</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><tok cat="NN">oriP</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">mapped</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">1.955-kb</tok> <tok cat="JJ">viral</tok> <tok cat="NN">segment</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
862769507@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="JJ">viral</tok> <tok cat="NN">fragment</tok></cons> <cons cat="VP"><tok cat="VBZ">permits</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><cons cat="NP"><tok cat="JJ">stable</tok> <cons cat="NP"><tok cat="NN">plasmid</tok></cons> <tok cat="NN">replication</tok></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">deletion</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">rearrangement</tok></cons></cons></cons></cons> <cons cat="CONJP"><tok cat="RB">as</tok> <tok cat="RB">well</tok> <tok cat="IN">as</tok></cons> <cons cat="NP"><cons cat="NP"><tok cat="JJ">episomal</tok> <tok cat="NN">maintenance</tok></cons> <cons cat="PP"><tok cat="IN">without</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">integration</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">recombination</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010
862769508@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">function</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">oriP</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">depends</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><tok cat="JJ">trans-acting</tok> <tok cat="NN">factor</tok></cons><tok cat="LRB">(</tok><tok cat="NNS">s</tok><tok cat="RRB">)</tok></cons> <cons cat="VP"><tok cat="VBN">encoded</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">viral</tok> <tok cat="NN">genome</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@18@@oe@19-12-2010
862769509@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">The</tok> <cons cat="NP"><tok cat="JJ">1.955-kb</tok> <tok cat="JJ">viral</tok> <tok cat="NN">segment</tok></cons></cons> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VBZ">includes</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">dyad</tok> <tok cat="NN">symmetry</tok> <tok cat="NN">region</tok></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">between</tok> <cons cat="NP"><tok cat="CD">two</tok> <cons cat="NP"><tok cat="JJ">small</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">RNA</tok> <tok cat="NNS">genes</tok></cons></cons></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="ADVP"><tok cat="RB">upstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">dihydrofolate</tok> <tok cat="NN">reductase</tok> <tok cat="NN">gene</tok> <tok cat="NN">homolog</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
862769510@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Therefore</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <cons cat="NP"><tok cat="NN">oriP</tok></cons></cons> <cons cat="VP"><tok cat="VBZ">contains</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">novel</tok> <tok cat="NNS">elements</tok></cons> <cons cat="ADJP"><tok cat="JJ">distinct</tok> <cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">those</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">other</tok> <tok cat="NN">DNA</tok> <tok cat="NNS">viruses</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
862770101@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Cyclic</tok> <tok cat="JJ">AMP-responsive</tok> <tok cat="JJ">element-dependent</tok> <tok cat="NN">activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok> <cons cat="NP"><tok cat="NN">zebra</tok> <tok cat="NN">promoter</tok></cons></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="JJ">human</tok> <tok cat="NN">herpesvirus</tok> <tok cat="CD">6</tok></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@14@@oe@19-12-2010
862770102@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">have</tok> <cons cat="ADVP" adv="TMP"><tok cat="RB">recently</tok></cons> <cons cat="VP"><tok cat="VBN">shown</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">infection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">Epstein-Barr</tok> <tok cat="NN">virus</tok> <tok cat="LRB">(</tok><tok cat="NN">EBV</tok><tok cat="RRB">)</tok> <tok cat="JJ">genome-positive</tok> <tok cat="NN">B</tok> <tok cat="NNS">cells</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">herpesvirus</tok> <tok cat="CD">6</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">HHV-6</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">results</tok> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">immediate-early</tok> <tok cat="NN">EBV</tok> <tok cat="NN">Zebra</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">followed</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">virus</tok> <tok cat="NN">replication</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NNP">L.Flamand</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">I.Stefanescu</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">D.V.Ablashi</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NNP">J.Menezes</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NNP">J.Virol.67</tok></cons><tok cat="COLON">:</tok><cons cat="NP"><tok cat="CD">6768-6777</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="CD">1993</tok></cons></cons><tok cat="RRB">)</tok></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@54@@oe@19-12-2010
862770103@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Here</tok></cons> <cons cat="NP" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">show</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="NN">HHV-6</tok></cons> <cons cat="VP"><tok cat="VBZ">upregulates</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Zebra</tok> <tok cat="NN">gene</tok></cons> <tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">through</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cyclic</tok> <tok cat="JJ">AMP-responsive</tok> <tok cat="NN">element</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CRE</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <cons cat="ADJP"><tok cat="JJ">located</tok> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">Zebra</tok> <tok cat="NN">promoter</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">Zp</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
862770104@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">Using</tok> <cons cat="NP"><cons cat="NP" syn="COOD"><tok cat="JJ">human</tok> <cons cat="NP"><tok cat="NN">B-</tok> <cons cat="NP" null="QSTN"></cons><cons cat="NP" ref="i20" null="RNR"></cons></cons><tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">T-cell</tok> <cons cat="NP" ref="i20" null="RNR"></cons></cons><cons cat="NP" id="i20"><tok cat="NNS">lines</tok></cons></cons> <cons cat="VP"><tok cat="VBN">transfected</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">ZpCat</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok> <tok cat="NNS">constructs</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">region</tok></cons> <cons cat="VP"><tok cat="VBN">designated</tok> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">ZII</tok> <tok cat="NN">domain</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Zp</tok></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">target</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">HHV-6</tok> <tok cat="NN">transactivation</tok></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@31@@oe@19-12-2010
862770105@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Mutation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">consensus</tok> <tok cat="NN">AP-1/CRE</tok> <tok cat="NN">site</tok></cons></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="NN">ZII</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">abolished</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">inducibility</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Zp</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">HHV-6</tok></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR" func="ADV"><tok cat="IN">whereas</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">positioning</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">ZII</tok> <tok cat="JJ">domain</tok></cons></cons></cons> <cons cat="ADVP"><tok cat="JJ">upstream</tok> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">beta-globin</tok> <tok cat="JJ">minimal</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">conferred</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">responsiveness</tok></cons> <cons cat="PP"><tok cat="VBG">following</tok> <cons cat="NP"><tok cat="NN">HHV-6</tok> <tok cat="NN">infection</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@34@@oe@19-12-2010
862770106@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i8" role="SBJ"><cons cat="NP"><tok cat="NN">Binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">these</tok> <tok cat="NNS">factors</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">ZII</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">prevented</tok> <cons cat="NP" ref="i8" null="NONE"></cons><cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NNS">oligonucleotides</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><tok cat="NN">CRE</tok></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">AP-1</tok> <tok cat="NN">consensus</tok> <tok cat="NNS">sequences</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@19@@oe@19-12-2010
862770107@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i9" role="SBJ"><cons cat="NP"><tok cat="NNS">Antibodies</tok></cons> <cons cat="PP" syn="COOD"><cons cat="PP"><tok cat="IN">against</tok> <cons cat="NP"><tok cat="JJ">CRE-binding</tok> <tok cat="LRB">(</tok><tok cat="NN">CREB</tok><tok cat="RRB">)</tok> <tok cat="NN">protein</tok></cons></cons> <tok cat="CC">but</tok> <cons cat="PP"><tok cat="RB">not</tok> <tok cat="IN">against</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">c-Fos</tok></cons> <tok cat="CC">or</tok> <cons cat="NP"><tok cat="NN">c-Jun</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i9" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">supershift</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">DNA-protein</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">identifying</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">nature</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons></cons> <cons cat="SBAR"><cons cat="WHNP" id="i10"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i10" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBZ">binds</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">ZII</tok></cons></cons> <cons cat="PP"><tok cat="IN">as</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">member</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CREB</tok> <tok cat="NN">family</tok> <tok cat="IN">of</tok> <tok cat="NNS">proteins</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@19-12-2010
862770108@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Finally</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i11" role="SBJ"><cons cat="NP"><tok cat="NN">transfection</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">CREB</tok> <tok cat="NN">protein</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">A</tok> <tok cat="NN">expression</tok> <tok cat="NNS">vectors</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">were</tok> <cons cat="VP"><tok cat="VBN">found</tok> <cons cat="S"><cons cat="NP" ref="i11" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="NN">Zp</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="NN">Jurkat</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBG">suggesting</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">phosphorylated</tok> <tok cat="NN">form</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CREB</tok> <tok cat="NN">protein</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">play</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">determining</tok> <tok cat="NN">role</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">EBV</tok> <tok cat="NN">reactivation</tok> <tok cat="NN">process</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@39@@oe@19-12-2010
862828401@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="JJ">Genetic</tok> <tok cat="NN">characterization</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok> <tok cat="NN">promoter</tok></cons></cons></cons></cons></cons><tok cat="COLON">:</tok></cons> <cons cat="S"><cons cat="NP" id="i5" role="SBJ"><cons cat="NP"><tok cat="NN">Binding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Tax</tok></cons></cons> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="JJ">Tax-responsive</tok> <tok cat="NN">element</tok> <tok cat="CD">1</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="VP"><tok cat="VBN">mediated</tok> <cons cat="NP" ref="i5" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">cyclic</tok> <tok cat="JJ">AMP-responsive</tok> <tok cat="NNS">members</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CREB/ATF</tok> <tok cat="NN">family</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">transcription</tok> <tok cat="NNS">factors</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@36@@oe@19-12-2010
862828402@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" ref="i4" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">achieve</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJR">better</tok> <tok cat="NN">understanding</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">Tax</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NN">T-cell</tok> <tok cat="NN">leukemia</tok> <tok cat="NN">virus</tok> <tok cat="NN">type</tok> <tok cat="CD">1</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">Tax-responsive</tok> <tok cat="NN">element</tok> <tok cat="CD">1</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">TRE-1</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i4" role="SBJ"><tok cat="PRP">we</tok></cons> <cons cat="VP"><tok cat="VBD">developed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">genetic</tok> <tok cat="NN">approach</tok></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">Saccharomyces</tok> <tok cat="NN">cerevisiae</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons></cons>@@@@1@35@@oe@19-12-2010
862828403@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBD">constructed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">yeast</tok> <tok cat="NN">reporter</tok> <tok cat="NN">strain</tok></cons> <cons cat="VP"><tok cat="VBG">containing</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">lacZ</tok> <tok cat="NN">gene</tok></cons></cons> <cons cat="PP"><tok cat="IN">under</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">control</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CYC1</tok> <tok cat="NN">promoter</tok></cons></cons> <cons cat="VP"><tok cat="VBN">associated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">three</tok> <tok cat="NNS">copies</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">TRE-1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@24@@oe@19-12-2010
862828404@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><tok cat="CC">either</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cyclic</tok> <tok cat="NN">AMP</tok> <tok cat="NN">response</tok> <tok cat="NN">element-binding</tok> <tok cat="NN">protein</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CREB</tok></cons><tok cat="RRB">)</tok></cons></cons></cons> <tok cat="CC">or</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">CREB</tok></cons> <cons cat="VP"><tok cat="VBN">fused</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">GAL4</tok> <tok cat="NN">activation</tok> <tok cat="NN">domain</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">GAD</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">strain</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">modify</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@36@@oe@19-12-2010
862828405@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Tax</tok></cons> <cons cat="ADVP"><tok cat="RB">alone</tok></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="ADJP" role="PRD"><tok cat="JJ">inactive</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@6@@oe@19-12-2010
862828406@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">However</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i6" role="SBJ"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">reporter</tok> <tok cat="NN">gene</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="VP"><tok cat="VBN">induced</tok> <cons cat="NP" ref="i6" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><cons cat="NP"><tok cat="NN">coexpression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">Tax</tok></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CREB</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
862828407@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok> <tok cat="NN">effect</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJR">stronger</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">GAD-CREB</tok> <tok cat="NN">fusion</tok> <tok cat="NN">protein</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@19-12-2010
862828408@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Analysis</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">different</tok> <cons cat="NP"><tok cat="NN">CREB</tok> <tok cat="NNS">mutants</tok></cons></cons> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="JJ">genetic</tok> <tok cat="NN">system</tok></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">indicated</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">C-terminal</tok> <tok cat="CD">92</tok> <tok cat="NN">amino</tok> <tok cat="NN">acid</tok> <tok cat="NNS">residues</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><cons cat="WHNP" id="i7"><tok cat="WDT">which</tok></cons> <cons cat="S"><cons cat="NP" ref="i7" role="SBJ" null="T"></cons><cons cat="VP"><tok cat="VBP">include</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">basic</tok> <tok cat="NN">domain</tok></cons></cons> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">leucine</tok> <tok cat="NN">zipper</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok></cons> <cons cat="VP"><tok cat="VBP">are</tok> <cons cat="ADJP" syn="COOD"><cons cat="ADJP"><tok cat="JJ">necessary</tok></cons> <tok cat="CC">and</tok> <cons cat="ADJP"><tok cat="JJ">sufficient</tok></cons></cons> <cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">mediate</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">transactivation</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">Tax</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@38@@oe@19-12-2010
862828409@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">To</tok> <cons cat="VP"><tok cat="VB">identify</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">cellular</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="VBG">binding</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">TRE-1</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">Tax-dependent</tok> <tok cat="NN">manner</tok></cons></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i8" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">strain</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADVP"><tok cat="RB">also</tok></cons> <cons cat="VP"><tok cat="VBN">used</tok> <cons cat="NP" ref="i8" null="NONE"></cons></cons><cons cat="S" func="ADV"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">screen</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">library</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">human</tok> <tok cat="NNS">cDNAs</tok></cons> <cons cat="VP"><tok cat="VBN">fused</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">GAD</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@28@@oe@19-12-2010
862828410@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="S"><cons cat="PP"><tok cat="IN">Of</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">five</tok> <tok cat="JJ">positive</tok> <tok cat="NNS">clones</tok></cons> <cons cat="VP"><tok cat="VBN">isolated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">from</tok> <cons cat="NP"><cons cat="QP"><tok cat="CD">0.75</tok> <tok cat="CC">x</tok> <tok cat="CD">10(6)</tok></cons> <tok cat="NN">yeast</tok> <tok cat="NNS">colonies</tok></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="CD">four</tok> <cons cat="NP" ref="i30" null="ICH"></cons></cons><cons cat="VP"><tok cat="VBD">were</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="NNS">members</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">CREB/activating</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok> <tok cat="LRB">(</tok><tok cat="NN">ATF</tok><tok cat="RRB">)</tok> <tok cat="NN">family</tok></cons></cons></cons></cons></cons></cons><tok cat="COLON">:</tok> <cons cat="NP" syn="COOD" id="i30"><cons cat="NP"><tok cat="NN">CREB</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="CD">two</tok> <tok cat="NNS">isoforms</tok> <tok cat="IN">of</tok> <tok cat="DT">the</tok> <tok cat="JJ">cyclic</tok> <tok cat="JJ">AMP-responsive</tok> <tok cat="NN">element</tok> <tok cat="NN">modulator</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CREM</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">ATF-1</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@42@@oe@19-12-2010
862828411@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Interestingly</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i9" role="SBJ"><tok cat="DT">these</tok> <tok cat="CD">three</tok> <tok cat="NNS">proteins</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP" syn="COOD"><cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">phosphorylated</tok> <cons cat="NP" ref="i9" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">protein</tok> <tok cat="NN">kinase</tok> <tok cat="NN">A</tok></cons></cons></cons></cons> <tok cat="CC">and</tok> <cons cat="ADVP"><tok cat="RB">thus</tok></cons> <cons cat="VP"><tok cat="VB">form</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">particular</tok> <tok cat="NN">subgroup</tok></cons> <cons cat="PP"><tok cat="IN">within</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">CREB/ATF</tok> <tok cat="NN">family</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@23@@oe@19-12-2010
862828412@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="NN">Expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">ATF-2</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="FW">S.</tok> <tok cat="FW">cerevisiae</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">did</tok> <tok cat="RB">not</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="NN">TRE-1</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">presence</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Tax</tok></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@16@@oe@19-12-2010
862828413@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">This</tok></cons> <cons cat="VP"><tok cat="VBZ">shows</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">eukaryotic</tok> <tok cat="NN">nucleus</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="NN">Tax</tok></cons> <cons cat="ADVP"><tok cat="RB">specifically</tok></cons> <cons cat="VP"><tok cat="VBZ">interacts</tok> <cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">basic</tok> <tok cat="JJ">domain-leucine</tok> <tok cat="NN">zipper</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">ATF-1</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP"><tok cat="NN">CREB</tok></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">CREM</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@25@@oe@19-12-2010
862828414@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="JJ">fifth</tok> <tok cat="NN">clone</tok></cons> <cons cat="VP"><tok cat="VBN">identified</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">this</tok> <tok cat="NN">screening</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">corresponded</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="NN">Ku</tok> <tok cat="NN">autoantigen</tok> <tok cat="NN">p70</tok> <tok cat="NN">subunit</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
862828415@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="SBAR" func="ADV" adv="TMP"><cons cat="WHADVP" id="i10"><tok cat="WRB">When</tok></cons> <cons cat="S"><cons cat="NP" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="VBN">fused</tok> <cons cat="NP" ref="i16" null="NONE"></cons><cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">GAD</tok></cons></cons><cons cat="ADVP" ref="i10" null="T"></cons></cons></cons></cons><tok cat="COMMA">,</tok> <cons cat="S"><cons cat="NP" id="i16" role="SBJ"><cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><tok cat="JJ">C-terminal</tok> <tok cat="NN">region</tok></cons></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">Ku</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">able</tok> <cons cat="S"><cons cat="NP" ref="i16" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><tok cat="NN">transcription</tok></cons> <cons cat="PP"><tok cat="IN">via</tok> <cons cat="NP"><tok cat="NN">TRE-1</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons> <tok cat="CC">but</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <tok cat="RB">not</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">dependent</tok> <cons cat="PP"><tok cat="IN">on</tok> <cons cat="NP"><tok cat="NN">Tax</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
863180901@GENIA Treebank@formal@@1@S@<cons cat="NP"><cons cat="NP"><tok cat="NN">Activation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="NN">cyclosporin</tok> <tok cat="JJ">A-resistant</tok></cons> <tok cat="NN">pathway</tok></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@14@@oe@19-12-2010
863180902@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="DT">The</tok> <tok cat="NN">mechanism</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">action</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">immunosuppressive</tok> <tok cat="NN">drug</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">cyclosporin</tok> <tok cat="NN">A</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">CsA</tok></cons><tok cat="RRB">)</tok></cons></cons></cons></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="NP" role="PRD"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">inactivation</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">Ca2+/calmodulin-dependent</tok> <tok cat="NN">serine-threonine</tok> <tok cat="NN">phosphatase</tok> <tok cat="NN">calcineurin</tok></cons></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">drug-immunophilin</tok> <tok cat="NN">complex</tok></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
863180903@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i49" role="SBJ"><tok cat="JJ">Inactive</tok> <tok cat="NN">calcineurin</tok></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">unable</tok> <cons cat="S"><cons cat="NP" ref="i49" role="SBJ" null="NONE"></cons><cons cat="VP"><tok cat="TO">to</tok> <cons cat="VP"><tok cat="VB">activate</tok> <cons cat="NP"><cons cat="NP"><cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NN">factor</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="VBN">activated</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">NFAT</tok></cons><tok cat="RRB">)</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">a</tok> <tok cat="NN">transcription</tok> <tok cat="NN">factor</tok></cons> <cons cat="VP"><tok cat="VBN">required</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">for</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">expression</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">the</tok> <cons cat="NP"><cons cat="NP"><tok cat="NN">interleukin</tok> <tok cat="CD">2</tok></cons> <cons cat="PRN"><tok cat="LRB">(</tok><cons cat="NP"><tok cat="NN">IL-2</tok></cons><tok cat="RRB">)</tok></cons></cons> <tok cat="NN">gene</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@32@@oe@19-12-2010
863180904@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" id="i50" role="SBJ"><cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">production</tok></cons> <cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP"><tok cat="JJ">CsA-treated</tok> <tok cat="NNS">cells</tok></cons></cons></cons> <cons cat="VP"><tok cat="VBZ">is</tok> <cons cat="ADVP"><tok cat="RB">therefore</tok></cons> <cons cat="VP"><cons cat="ADVP"><tok cat="RB">dramatically</tok></cons> <tok cat="VBN">reduced</tok><cons cat="NP" ref="i50" null="NONE"></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@10@@oe@19-12-2010
863180905@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="PRP">We</tok></cons> <cons cat="VP"><tok cat="VBP">demonstrate</tok> <cons cat="ADVP"><tok cat="RB">here</tok></cons><tok cat="COMMA">,</tok> <cons cat="ADVP"><tok cat="RB">however</tok></cons><tok cat="COMMA">,</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i51" role="SBJ"><tok cat="NN">NFAT</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">activated</tok><cons cat="NP" ref="i51" null="NONE"></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i52" role="SBJ"><cons cat="NP"><tok cat="JJ">significant</tok> <tok cat="NNS">levels</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons></cons></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" ref="i52" null="NONE"></cons></cons></cons></cons></cons></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="DT">the</tok> <tok cat="JJ">CsA-resistant</tok> <tok cat="JJ">CD28-signaling</tok> <tok cat="NN">pathway</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@26@@oe@19-12-2010
863180906@GENIA Treebank@formal@@1@S@<cons cat="S" syn="COOD"><cons cat="S"><cons cat="PP"><tok cat="IN">In</tok> <cons cat="NP"><tok cat="JJ">transient</tok> <tok cat="NN">transfection</tok> <tok cat="NNS">assays</tok></cons></cons><tok cat="COMMA">,</tok> <cons cat="NP" id="i53" role="SBJ"><cons cat="NP" syn="COOD"><tok cat="CC">both</tok> <cons cat="NP"><tok cat="JJ">multicopy</tok> <tok cat="NN">NFAT-</tok> <cons cat="NP" ref="i54" null="RNR"></cons></cons><tok cat="CC">and</tok> <cons cat="NP"><tok cat="NN">IL-2</tok> <tok cat="NN">promoter-beta-galactosidase</tok> <tok cat="NN">reporter</tok> <tok cat="NN">gene</tok> <cons cat="NP" ref="i54" null="RNR"></cons></cons></cons><cons cat="NP" id="i54"><tok cat="NNS">constructs</tok></cons></cons> <cons cat="VP"><tok cat="MD">could</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i53" null="NONE"></cons><cons cat="PP"><tok cat="IN">by</tok> <cons cat="NP" role="LGS"><tok cat="NN">phorbol</tok> <tok cat="NN">12-myristate</tok> <tok cat="NN">13-acetate</tok> <tok cat="LRB">(</tok><tok cat="NN">PMA</tok><tok cat="RRB">)</tok><tok cat="NN">/alpha-CD28</tok> <tok cat="NN">stimulation</tok></cons></cons></cons></cons></cons></cons><tok cat="COMMA">,</tok> <tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" role="SBJ"><tok cat="DT">this</tok> <tok cat="NN">activation</tok></cons> <cons cat="VP"><tok cat="VBD">was</tok> <cons cat="ADJP" role="PRD"><tok cat="JJ">resistant</tok> <cons cat="PP"><tok cat="TO">to</tok> <cons cat="NP"><tok cat="NN">CsA</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@35@@oe@19-12-2010
863180907@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><tok cat="JJ">Electrophoretic</tok> <tok cat="NN">mobility</tok> <tok cat="NN">shift</tok> <tok cat="NN">assay</tok></cons> <cons cat="VP"><tok cat="VBD">showed</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="NN">induction</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><tok cat="DT">a</tok> <tok cat="JJ">CsA-resistant</tok> <tok cat="NN">NFAT</tok> <tok cat="NN">complex</tok></cons></cons> <cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><cons cat="NP"><tok cat="DT">the</tok> <tok cat="JJ">nuclear</tok> <tok cat="NNS">extracts</tok></cons> <cons cat="PP"><tok cat="IN">of</tok> <cons cat="NP"><cons cat="NP"><tok cat="JJ">peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP" syn="COOD"><cons cat="NP"><tok cat="NN">PMA</tok></cons> <tok cat="CC">plus</tok> <cons cat="NP"><tok cat="NN">alphaCD28</tok></cons></cons></cons></cons></cons></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@27@@oe@19-12-2010
863180908@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="NP" role="SBJ"><cons cat="NP"><tok cat="JJ">Peripheral</tok> <tok cat="NN">blood</tok> <tok cat="NN">T</tok> <tok cat="NNS">cells</tok></cons> <cons cat="VP"><tok cat="VBN">stimulated</tok> <cons cat="NP" null="NONE"></cons><cons cat="PP"><tok cat="IN">with</tok> <cons cat="NP"><tok cat="NN">PMA/alphaCD28</tok></cons></cons></cons></cons> <cons cat="VP"><tok cat="VBD">produced</tok> <cons cat="NP"><tok cat="NN">IL-2</tok></cons> <cons cat="PP"><tok cat="IN">in</tok> <tok cat="DT">the</tok> <tok cat="NN">presence</tok> <tok cat="IN">of</tok> <cons cat="NP"><tok cat="NN">CsA</tok></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@15@@oe@19-12-2010
863180909@GENIA Treebank@formal@@1@S@<cons cat="S"><cons cat="ADVP"><tok cat="RB">Collectively</tok></cons><tok cat="COMMA">,</tok> <cons cat="NP" role="SBJ"><tok cat="DT">these</tok> <tok cat="NNS">data</tok></cons> <cons cat="VP"><tok cat="VBP">suggest</tok> <cons cat="SBAR"><tok cat="IN">that</tok> <cons cat="S"><cons cat="S" syn="COOD"><cons cat="S"><cons cat="NP" id="i55" role="SBJ"><tok cat="NN">NFAT</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">activated</tok> <cons cat="NP" ref="i55" null="NONE"></cons></cons></cons></cons></cons><tok cat="CC">and</tok> <cons cat="S"><cons cat="NP" id="i56" role="SBJ"><tok cat="NN">IL-2</tok></cons> <cons cat="VP"><tok cat="MD">can</tok> <cons cat="VP"><tok cat="VB">be</tok> <cons cat="VP"><tok cat="VBN">produced</tok> <cons cat="NP" ref="i56" null="NONE"></cons></cons></cons></cons></cons></cons><cons cat="PP"><tok cat="IN">in</tok> <cons cat="NP"><tok cat="DT">a</tok> <cons cat="ADJP"><tok cat="NN">calcineurin</tok> <tok cat="JJ">independent</tok></cons> <tok cat="NN">manner</tok></cons></cons></cons></cons></cons><tok cat="PERIOD">.</tok></cons>@@@@1@21@@oe@19-12-2010